FvH4_2g39100

Abhydrolase domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0004797
Physical Location & Seq
Reverse (-)
28151786 .. 28164464
12679 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g39100.t10

Sequence Viewer

Length: 1299 bp
ATGAACCTGCGTAGACTCTCTTTCAGAATGGCCGAAGAAATCTCCAGGTCCGCCTCCGACCTCCGACCCTCTGCCTCCACCGCCGCCGTCGCCGCCGCCGCCACAGAGTCAAAGTCAAAATATTTCTGGCCCTCCGTCCTCCGCTGGATCCCCACCTCCACCGATCACATCATCGCCGCCGAGAATCGCCTCCTCTCTATCGTCAAGACTCCGTATGTGGTGGAGCGGGTCAATATCGGGTCGGGCCCGCCCGGGTCGAACGTGAGGTGGTTCAGGTCTGCAAGCAATGAGGAGAGGTTTATCAACACGGTCACTTTTGACAGCAAAGACGAGTCTCCTACTATTGTGATGGTTCATGGATACGGTTGCTCGCAAGGTTTCTTCTTCAGGAACTTTGATGCTCTGGCGAGTCGGTTCAGGGTTATTGCTATTGATCAACTTGGCTGGGGTGGATCAAGCAGACCCGATTTTACATGCAAAAGCACTGAAGAAACCGAGGCATGGTTTATTGATTCTTTGGAAGAATGGCGGAAAGCCAAAAATCTCAGCAACTTTATATTACTTGGACATTCATTTGGTGGCTATGTTGCATCCAAGTATGCTCTAAAGCATCCGGAGCACATTAAGCATTTGGTTTTAGTGGGTCCTGTTGGTTTCTCCTCGGCAGAAGATGCAAGGAATGAACGGATCATGCAGTTCAAAACAACATGGAAAGGAGCAATTATTAACCATTTGTGGGAGTCTAATTTTACTCCTCAGAAATTAATCAGAGGGTTAGGGCCTTGGGGTCCAAAGATTGTTCGGGGTTACACAAATAGTAGATTTGGCGCTCGTGATGCAGGGACCACCCTGAATGAGCATGAAGCCCAACTGCTAACAGATTACATGTACCATACTTTAGCTGCCAAAGCAAGTGGAGAGTTGTGCTTGAAATATATTTTTTCTTTCGGAGCATTTGCTCGGAAGCCTCTTCTTCAGAGTGCACCAGAGTGGAAAGTTCCAACTACCTTTATTTATGGCTATCAAGATTGGAGTAATTATCAAGGGGCTCAAGAAGCTCGCAAGGATATGAAGGTCCCGTGTGAGATTATTAGAGTTCCTGAGGCGGGACACTATGTATTCATAGACAATCCGGAGGGTTTCCATTCAGCTGTTTTCCACGCTTGCCGGAGATTTCTGTCACCTGACCCAGACAATGAATCCTTGCCTGAAGTTTCATTTCCTAGGACTGTTGTGTTGCGAAAATTATTCAGCGCACCCGTATACATATGCACCCGGAATCTCCACCGTTCAAATTGA

Protein Analysis

433

Amino Acids

48.85

Weight (kDa)

9.16

Isoelectric Point (pI)

45.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Chlorophyllase PF07224 105 - 230 9.8e-06 Chlorophyllase
Abhydrolase_1 PF00561 113 - 229 7.5e-23 alpha/beta hydrolase fold
Hydrolase_4 PF12146 114 - 376 4.2e-18 Serine aminopeptidase, S33
Abhydrolase_6 PF12697 115 - 381 1.2e-20 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 15
AccBSI CCGCTC 1 cut(s) 226
AccI GTMKAC 2 cut(s) 13, 1263
AccIII TCCGGA 2 cut(s) 613, 1132
AclWI GGATC 4 cut(s) 142, 155, 460, 695
AcoI YGGCCR 1 cut(s) 30
AcuI CTGAAG 4 cut(s) 370, 507, 959, 1230
AfaI GTAC 1 cut(s) 890
AfiI CCNNNNNNNGG 3 cut(s) 501, 736, 1106
AflIII ACRYGT 1 cut(s) 885
AgsI TTSAA 3 cut(s) 700, 931, 1293
AjnI CCWGG 1 cut(s) 44
AleI CACNNNNGTG 1 cut(s) 988
AluBI AGCT 3 cut(s) 902, 1058, 1151
AluI AGCT 3 cut(s) 902, 1058, 1151
Alw21I GWGCWC 2 cut(s) 621, 985
Alw26I GTCTC 1 cut(s) 339
Alw44I GTGCAC 1 cut(s) 981
AlwI GGATC 4 cut(s) 142, 155, 460, 695
Ama87I CYCGRG 1 cut(s) 251
Aor13HI TCCGGA 2 cut(s) 613, 1132
AoxI GGCC 4 cut(s) 30, 128, 244, 779
ApaI GGGCCC 1 cut(s) 248
ApaLI GTGCAC 1 cut(s) 981
ApeKI GCWGC 1 cut(s) 902
AseI ATTAAT 1 cut(s) 764
AspA2I CCTAGG 1 cut(s) 1223
AspLEI GCGC 2 cut(s) 830, 1256
AspS9I GGNCC 9 cut(s) 48, 129, 244, 245, 644, 779, 788, 843, 1075
AsuC2I CCSGG 3 cut(s) 252, 253, 1276
AsuHPI GGTGA 1 cut(s) 1173
AvaI CYCGRG 1 cut(s) 251
AvaII GGWCC 5 cut(s) 48, 644, 788, 843, 1075
AvrII CCTAGG 1 cut(s) 1223
AxyI CCTNAGG 1 cut(s) 1101
BaeGI GKGCMC 2 cut(s) 248, 985
BamHI GGATCC 1 cut(s) 147
BanII GRGCYC 2 cut(s) 248, 1051
BauI CACGAG 1 cut(s) 831
Bbv12I GWGCWC 2 cut(s) 621, 985
BbvI GCAGC 1 cut(s) 889
BccI CCATC 1 cut(s) 343
BceAI ACGGC 1 cut(s) 71
BciT130I CCWGG 1 cut(s) 46
BciVI GTATCC 1 cut(s) 353
BclI TGATCA 1 cut(s) 433
BcnI CCSGG 3 cut(s) 252, 253, 1276
BcoDI GTCTC 1 cut(s) 339
BfaI CTAG 1 cut(s) 1224
BfoI RGCGCY 1 cut(s) 831
BfuAI ACCTGC 1 cut(s) 15
BfuI GTATCC 1 cut(s) 353
BisI GCNGC 6 cut(s) 84, 93, 96, 99, 177, 903
BlnI CCTAGG 1 cut(s) 1223
BlsI GCNGC 6 cut(s) 85, 94, 97, 100, 178, 904
Bme1390I CCNGG 4 cut(s) 46, 252, 253, 1276
Bme18I GGWCC 5 cut(s) 48, 644, 788, 843, 1075
BmeT110I CYCGRG 1 cut(s) 251
BmgT120I GGNCC 9 cut(s) 48, 129, 244, 245, 644, 779, 788, 843, 1075
BmiI GGNNCC 6 cut(s) 149, 246, 645, 789, 844, 1077
BmrFI CCNGG 4 cut(s) 46, 252, 253, 1276
BmsI GCATC 5 cut(s) 388, 599, 619, 661, 826
BpmI CTGGAG 1 cut(s) 28
BpuEI CTTGAG 1 cut(s) 1035
BpuMI CCSGG 3 cut(s) 252, 253, 1276
BsaJI CCNNGG 5 cut(s) 251, 495, 660, 782, 1223
BsaWI WCCGGW 2 cut(s) 613, 1132
Bsc4I CCNNNNNNNGG 3 cut(s) 501, 736, 1106
Bse21I CCTNAGG 1 cut(s) 1101
Bse3DI GCAATG 1 cut(s) 292
BseAI TCCGGA 2 cut(s) 613, 1132
BseBI CCWGG 1 cut(s) 46
BseDI CCNNGG 5 cut(s) 251, 495, 660, 782, 1223
BseGI GGATG 2 cut(s) 590, 610
BseLI CCNNNNNNNGG 3 cut(s) 501, 736, 1106
BseMI GCAATG 1 cut(s) 292
BseMII CTCAG 3 cut(s) 559, 770, 1092
BseRI GAGGAG 4 cut(s) 182, 305, 649, 744
BseSI GKGCMC 2 cut(s) 248, 985
BseXI GCAGC 1 cut(s) 889
BseYI CCCAGC 1 cut(s) 444
BshFI GGCC 4 cut(s) 32, 130, 246, 781
BsiHKAI GWGCWC 2 cut(s) 621, 985
BsiHKCI CYCGRG 1 cut(s) 251
BsiSI CCGG 5 cut(s) 252, 614, 1133, 1168, 1276
BslFI GGGAC 3 cut(s) 856, 1061, 1122
BslI CCNNNNNNNGG 3 cut(s) 501, 736, 1106
BsmAI GTCTC 1 cut(s) 339
BsmFI GGGAC 3 cut(s) 856, 1061, 1122
BsnI GGCC 4 cut(s) 32, 130, 246, 781
BsoBI CYCGRG 1 cut(s) 251
Bsp120I GGGCCC 1 cut(s) 244
Bsp1286I GDGCHC 4 cut(s) 248, 621, 985, 1051
Bsp13I TCCGGA 2 cut(s) 613, 1132
Bsp143I GATC 5 cut(s) 147, 163, 433, 452, 687
BspANI GGCC 4 cut(s) 32, 130, 246, 781
BspCNI CTCAG 3 cut(s) 558, 769, 1093
BspEI TCCGGA 2 cut(s) 613, 1132
BspLI GGNNCC 6 cut(s) 149, 246, 645, 789, 844, 1077
BspMI ACCTGC 1 cut(s) 15
BspPI GGATC 4 cut(s) 142, 155, 460, 695
BsrBI CCGCTC 1 cut(s) 226
BsrDI GCAATG 1 cut(s) 292
BssECI CCNNGG 5 cut(s) 251, 495, 660, 782, 1223
BssMI GATC 5 cut(s) 147, 163, 433, 452, 687
BssNAI GTATAC 1 cut(s) 1264
BssSI CACGAG 1 cut(s) 831
BssT1I CCWWGG 2 cut(s) 782, 1223
Bst1107I GTATAC 1 cut(s) 1264
Bst2BI CACGAG 1 cut(s) 831
Bst2UI CCWGG 1 cut(s) 46
Bst4CI ACNGT 4 cut(s) 310, 365, 1231, 1289
Bst6I CTCTTC 1 cut(s) 975
BstAPI GCANNNNNTGC 1 cut(s) 671
BstC8I GCNNGC 5 cut(s) 248, 283, 371, 1060, 1165
BstDEI CTNAG 3 cut(s) 545, 756, 1101
BstF5I GGATG 2 cut(s) 590, 610
BstH2I RGCGCY 1 cut(s) 831
BstHHI GCGC 2 cut(s) 830, 1256
BstKTI GATC 5 cut(s) 150, 166, 436, 455, 690
BstMAI GTCTC 1 cut(s) 339
BstMBI GATC 5 cut(s) 147, 163, 433, 452, 687
BstNI CCWGG 1 cut(s) 46
BstNSI RCATGY 2 cut(s) 477, 889
BstSCI CCNGG 4 cut(s) 44, 250, 251, 1274
BstSLI GKGCMC 2 cut(s) 248, 985
BstV1I GCAGC 1 cut(s) 889
BstX2I RGATCY 1 cut(s) 147
BstYI RGATCY 1 cut(s) 147
BstZ17I GTATAC 1 cut(s) 1264
Bsu36I CCTNAGG 1 cut(s) 1101
BsuI GTATCC 1 cut(s) 353
BsuRI GGCC 4 cut(s) 32, 130, 246, 781
BtgZI GCGATG 1 cut(s) 157
BtsCI GGATG 2 cut(s) 590, 610
BtsIMutI CAGTG 1 cut(s) 483
BveI ACCTGC 1 cut(s) 15
Cac8I GCNNGC 5 cut(s) 248, 283, 371, 1060, 1165
CfoI GCGC 2 cut(s) 830, 1256
Cfr13I GGNCC 9 cut(s) 48, 129, 244, 245, 644, 779, 788, 843, 1075
Cfr9I CCCGGG 1 cut(s) 251
Csp6I GTAC 1 cut(s) 889
CspCI CAANNNNNGTGG 2 cut(s) 895, 930
CviAII CATG 7 cut(s) 356, 474, 501, 691, 708, 860, 886
CviQI GTAC 1 cut(s) 889
DdeI CTNAG 3 cut(s) 545, 756, 1101
DpnI GATC 5 cut(s) 149, 165, 435, 454, 689
DpnII GATC 5 cut(s) 147, 163, 433, 452, 687
EaeI YGGCCR 1 cut(s) 30
Eam1104I CTCTTC 1 cut(s) 975
EarI CTCTTC 1 cut(s) 975
EciI GGCGGA 2 cut(s) 40, 544
Eco130I CCWWGG 2 cut(s) 782, 1223
Eco24I GRGCYC 2 cut(s) 248, 1051
Eco47I GGWCC 5 cut(s) 48, 644, 788, 843, 1075
Eco57I CTGAAG 4 cut(s) 370, 507, 959, 1230
Eco81I CCTNAGG 1 cut(s) 1101
Eco88I CYCGRG 1 cut(s) 251
EcoO109I RGGNCCY 3 cut(s) 644, 779, 1075
EcoRII CCWGG 1 cut(s) 44
EcoT14I CCWWGG 2 cut(s) 782, 1223
EcoT38I GRGCYC 2 cut(s) 248, 1051
ErhI CCWWGG 2 cut(s) 782, 1223
FaeI CATG 7 cut(s) 359, 477, 504, 694, 711, 863, 889
FaqI GGGAC 3 cut(s) 856, 1061, 1122
FatI CATG 7 cut(s) 355, 473, 500, 690, 707, 859, 885
FauI CCCGC 3 cut(s) 219, 255, 1099
FauNDI CATATG 1 cut(s) 1268
FbaI TGATCA 1 cut(s) 433
FblI GTMKAC 2 cut(s) 13, 1263
Fnu4HI GCNGC 6 cut(s) 84, 93, 96, 99, 177, 903
FokI GGATG 2 cut(s) 577, 597
FriOI GRGCYC 2 cut(s) 248, 1051
Fsp4HI GCNGC 6 cut(s) 84, 93, 96, 99, 177, 903
FspBI CTAG 1 cut(s) 1224
GlaI GCGC 2 cut(s) 829, 1255
GluI GCNGC 6 cut(s) 84, 93, 96, 99, 177, 903
GsaI CCCAGC 1 cut(s) 448
GsuI CTGGAG 1 cut(s) 28
HaeII RGCGCY 1 cut(s) 831
HaeIII GGCC 4 cut(s) 32, 130, 246, 781
HapII CCGG 5 cut(s) 252, 614, 1133, 1168, 1276
HhaI GCGC 2 cut(s) 830, 1256
Hin1II CATG 7 cut(s) 359, 477, 504, 694, 711, 863, 889
Hin6I GCGC 2 cut(s) 828, 1254
HinP1I GCGC 2 cut(s) 828, 1254
HpaII CCGG 5 cut(s) 252, 614, 1133, 1168, 1276
HphI GGTGA 1 cut(s) 1173
Hpy166II GTNNAC 3 cut(s) 14, 983, 1264
Hpy188I TCNGA 8 cut(s) 26, 58, 65, 759, 770, 950, 963, 978
Hpy188III TCNNGA 8 cut(s) 205, 388, 614, 833, 1025, 1052, 1100, 1133
Hpy8I GTNNAC 3 cut(s) 14, 983, 1264
Hpy99I CGWCG 1 cut(s) 92
HpyAV CCTTC 1 cut(s) 1066
HpyCH4III ACNGT 4 cut(s) 310, 365, 1231, 1289
HpyCH4IV ACGT 1 cut(s) 261
HpyCH4V TGCA 8 cut(s) 281, 477, 590, 674, 694, 839, 983, 1272
HpyF3I CTNAG 3 cut(s) 545, 756, 1101
HpySE526I ACGT 1 cut(s) 261
Hsp92II CATG 7 cut(s) 359, 477, 504, 694, 711, 863, 889
HspAI GCGC 2 cut(s) 828, 1254
Kpn2I TCCGGA 2 cut(s) 613, 1132
Ksp22I TGATCA 1 cut(s) 433
Kzo9I GATC 5 cut(s) 147, 163, 433, 452, 687
LmnI GCTCC 4 cut(s) 223, 616, 716, 950
Lsp1109I GCAGC 1 cut(s) 889
LweI GCATC 5 cut(s) 388, 599, 619, 661, 826
MaeI CTAG 1 cut(s) 1224
MaeII ACGT 1 cut(s) 261
MaeIII GTNAC 3 cut(s) 310, 806, 1179
MalI GATC 5 cut(s) 149, 165, 435, 454, 689
MbiI CCGCTC 1 cut(s) 226
MboI GATC 5 cut(s) 147, 163, 433, 452, 687
MboII GAAGA 8 cut(s) 47, 373, 376, 500, 533, 680, 962, 965
MflI RGATCY 1 cut(s) 147
MhlI GDGCHC 4 cut(s) 248, 621, 985, 1051
MluCI AATT 6 cut(s) 720, 745, 761, 1036, 1244, 1294
MlyI GAGTC 6 cut(s) 9, 116, 202, 341, 418, 749
MmeI TCCRAC 3 cut(s) 81, 88, 1025
MroI TCCGGA 2 cut(s) 613, 1132
MseI TTAA 3 cut(s) 624, 726, 764
MslI CAYNNNNRTG 1 cut(s) 988
MspA1I CMGCKG 2 cut(s) 144, 1151
MspI CCGG 5 cut(s) 252, 614, 1133, 1168, 1276
MspR9I CCNGG 4 cut(s) 46, 252, 253, 1276
MvaI CCWGG 1 cut(s) 46
NciI CCSGG 3 cut(s) 252, 253, 1276
NdeI CATATG 1 cut(s) 1268
NdeII GATC 5 cut(s) 147, 163, 433, 452, 687
NlaIII CATG 7 cut(s) 359, 477, 504, 694, 711, 863, 889
NlaIV GGNNCC 6 cut(s) 149, 246, 645, 789, 844, 1077
NmeAIII GCCGAG 2 cut(s) 205, 641
NmuCI GTSAC 2 cut(s) 310, 1179
NspI RCATGY 2 cut(s) 477, 889
OliI CACNNNNGTG 1 cut(s) 988
PciI ACATGT 1 cut(s) 885
PfeI GAWTC 4 cut(s) 184, 512, 1199, 1279
PkrI GCNGC 6 cut(s) 85, 94, 97, 100, 178, 904
PleI GAGTC 6 cut(s) 9, 115, 202, 340, 417, 748
PpsI GAGTC 6 cut(s) 9, 115, 202, 340, 417, 748
PpuMI RGGWCCY 2 cut(s) 644, 1075
PscI ACATGT 1 cut(s) 885
PshBI ATTAAT 1 cut(s) 764
Psp5II RGGWCCY 2 cut(s) 644, 1075
Psp6I CCWGG 1 cut(s) 44
PspFI CCCAGC 1 cut(s) 444
PspGI CCWGG 1 cut(s) 44
PspN4I GGNNCC 6 cut(s) 149, 246, 645, 789, 844, 1077
PspOMI GGGCCC 1 cut(s) 244
PspPI GGNCC 9 cut(s) 48, 129, 244, 245, 644, 779, 788, 843, 1075
PspPPI RGGWCCY 2 cut(s) 644, 1075
PsuI RGATCY 1 cut(s) 147
PvuII CAGCTG 1 cut(s) 1151
RsaI GTAC 1 cut(s) 890
RsaNI GTAC 1 cut(s) 889
RseI CAYNNNNRTG 1 cut(s) 988
SaqAI TTAA 3 cut(s) 624, 726, 764
SatI GCNGC 6 cut(s) 84, 93, 96, 99, 177, 903
Sau3AI GATC 5 cut(s) 147, 163, 433, 452, 687
Sau96I GGNCC 9 cut(s) 48, 129, 244, 245, 644, 779, 788, 843, 1075
SchI GAGTC 6 cut(s) 9, 116, 202, 341, 418, 749
ScrFI CCNGG 4 cut(s) 46, 252, 253, 1276
SduI GDGCHC 4 cut(s) 248, 621, 985, 1051
SfaNI GCATC 5 cut(s) 388, 599, 619, 661, 826
SinI GGWCC 5 cut(s) 48, 644, 788, 843, 1075
SmaI CCCGGG 1 cut(s) 253
SmiMI CAYNNNNRTG 1 cut(s) 988
SmlI CTYRAG 1 cut(s) 1050
SmoI CTYRAG 1 cut(s) 1050
Sse9I AATT 6 cut(s) 720, 745, 761, 1036, 1244, 1294
SspI AATATT 1 cut(s) 122
SspMI CTAG 1 cut(s) 1224
StyD4I CCNGG 4 cut(s) 44, 250, 251, 1274
StyI CCWWGG 2 cut(s) 782, 1223
TaaI ACNGT 4 cut(s) 310, 365, 1231, 1289
TaiI ACGT 1 cut(s) 264
TaqI TCGA 1 cut(s) 257
TasI AATT 6 cut(s) 720, 745, 761, 1036, 1244, 1294
TauI GCSGC 5 cut(s) 86, 95, 98, 101, 179
TfiI GAWTC 4 cut(s) 184, 512, 1199, 1279
Tru1I TTAA 3 cut(s) 624, 726, 764
Tru9I TTAA 3 cut(s) 624, 726, 764
TscAI CASTG 1 cut(s) 490
TseFI GTSAC 2 cut(s) 310, 1179
TseI GCWGC 1 cut(s) 902
Tsp45I GTSAC 2 cut(s) 310, 1179
TspDTI ATGAA 9 cut(s) 17, 344, 561, 696, 876, 1085, 1111, 1206, 1212
TspGWI ACGGA 3 cut(s) 124, 201, 700
TspMI CCCGGG 1 cut(s) 251
TspRI CASTG 1 cut(s) 490
VneI GTGCAC 1 cut(s) 981
VpaK11BI GGWCC 5 cut(s) 48, 644, 788, 843, 1075
VspI ATTAAT 1 cut(s) 764
XceI RCATGY 2 cut(s) 477, 889
XmaI CCCGGG 1 cut(s) 251
XmaJI CCTAGG 1 cut(s) 1223
XmiI GTMKAC 2 cut(s) 13, 1263
XspI CTAG 1 cut(s) 1224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.