Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0004797

Physical Location & Seq

Genomic Coordinates Forward (+)

964263 .. 967814

Gene Structure 3552 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1016400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1245 bp

ATGAACCTGCGTAGAATTTCGTCGAGAATGGCCGGCGAAATTAGCAGGTCCGCCGAGCAACGAACCTCCCCTGCCGCCGTTGCTGCAGCCTACGAATCCGATGCTGTTGCTGCTGCGGTTAATCCCAAGTCCAAATCGTTTTGGCCCTCTGTCCTCCGTTGGATCCCTACTTCCACCGATAAGATCATCGCCGCCGAGAAGCGCCTGCTCTCTCTTGTCAAGACTCCTTATGTTCAAGAACTGGTGAATATAGGATCTGGACCACCGGGCTCTAAAGTCAGGTGGTTTCGATCCGAAAGCAATGAAGAAAGGCAAATCAACACGGTTTCATTTGACAGCAAAGAGAATTCTCCTACTATTGTTATGATTCACGGATACGGTGCTTCTCAAGGTTTCTTCTTTAGAAATTTCGATGCCCTTGCCAGTCGGTTCAGGGTCCTTGCCATTGATCAACTAGGTTGGGGCGGATCAAGCAGGCCTGATTTTACATGTAAAAGCACTGAAGAAACTGAGGCATGGTTCATCGATTCCTTTGAGGAATGGCGAAAAGCCAAAAACCTCAGCAACTTTATATTACTTGGGCATTCAGTTGGTGGGTATGTTGCATCAAAGTACGCTCTGAAGCATCCAGAGCACGTTAAGCATTTGATTTTAGTGGGGCCTGTCGGGTTTTCATTGGAGTCGGATGCATCGTATGAGAGGATTAACCAATTCAAAGCAACATGGAAAGGAGCAATTTTCAACTATTTGTGGGAGTCTAATTTTACTCCACAGAAGTTAGTCAGAGGACTGGGGCCTTTGGGTCCAAATCTCGTTCGGAGATACACTACTGCCAGATTTGGTTCTTATTCAACAGGGACCGCACTAACTGAAGAGGAATCCCGACTTCTAACAGATTATGTGTACCATACTTTAGCAGCCAAAGCTAGTGGGGAGCTGTGCTTAAAGTACATATTATCTTTCGGAGCATTTGCTAGGAGTCCCCTTATACAGAGTGCATCGGAGTGGAAAGTGCCGACTACTTTTATTTACGGATATCAAGACTGGATGAACTACCAAGGGGCTCAAGAAGCTGGCAAAAAAATGAAGGTCCCATGTGAGATCATTAGGATTCCGGATGCTGGGCACTTTGTATTCATAGACCAACCCGAACGATTCCATTCAGCTGTTTTTTACGCTTGCCGGAGATTTCTTTCGCAGGACCCTGAGGTTGAATCCTTTCCCCAAGGTTTGACATCTGCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004620 GO:0004623 GO:0008150 GO:0016298 GO:0016740 GO:0016746 GO:0016747 GO:0016787 GO:0016788 GO:0042171 GO:0042592 GO:0048878 GO:0050801 GO:0052689 GO:0055081 GO:0055088 GO:0055089 GO:0055090 GO:0055091 GO:0065007 GO:0065008 GO:0070328 GO:0071617

KEGG Pathways

Metabolic & Signaling

ko04923 map04923

Pfam Domains

Protein Families

Abhydrolase_1

Protein Analysis

415

Amino Acids

46.45

Weight (kDa)

9.05

Isoelectric Point (pI)

49.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Hydrolase_4	PF12146	117 - 381	1.4e-18	Serine aminopeptidase, S33
Abhydrolase_1	PF00561	118 - 383	9.2e-29	alpha/beta hydrolase fold
Abhydrolase_6	PF12697	120 - 387	8.2e-21	Alpha/beta hydrolase family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004797)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G24160 AT4G24160
fragaria_vesca	FvH4_2g39100 FvH4_2g39100 FvH4_2g39100 FvH4_2g39100 FvH4_2g39100 FvH4_2g39100 FvH4_2g39100 FvH4_2g39100 FvH4_2g39100 FvH4_2g39100 FvH4_2g39100 FvH4_2g39100 FvH4_2g39100 FvH4_2g39100
malus_domestica	MD00G1162000.v1.1 MD08G1017500.v1.1 MD15G1016400.v1.1
prunus_persica	Prupe.1G370200_v2.0.a1 Prupe.1G370200_v2.0.a1
pyrus_communis	pycom08g01530 pycom15g01440
rosa_chinensis	RchiOBHm_Chr6g0302201
rosa_laevigata	RLG00000011187
rosa_multiflora	Rmu_ssc0000160.1_g000020
rosa_roxburghii	Rroxscaffold_7G00166000
rosa_rugosa	Rorug06G0313100
rosa_samantha	Rh6BG476900 Rh6DG425400
rosa_wichuraiana	Rw6G036860

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	15, 36
AccIII	TCCGGA	1 cut(s)	1114
AciI	CCGC	6 cut(s)	51, 75, 116, 192, 465, 861
AclWI	GGATC	5 cut(s)	157, 170, 262, 285, 475
AcoI	YGGCCR	1 cut(s)	30
AcsI	RAATTY	3 cut(s)	15, 346, 406
AcuI	CTGAAG	3 cut(s)	522, 641, 891
AfaI	GTAC	3 cut(s)	614, 905, 950
AfiI	CCNNNNNNNGG	1 cut(s)	1121
AflIII	ACRYGT	1 cut(s)	488
AgsI	TTSAA	5 cut(s)	236, 715, 742, 852, 1214
AluBI	AGCT	4 cut(s)	926, 937, 1073, 1166
AluI	AGCT	4 cut(s)	926, 937, 1073, 1166
Alw21I	GWGCWC	1 cut(s)	636
AlwI	GGATC	5 cut(s)	157, 170, 262, 285, 475
Aor13HI	TCCGGA	1 cut(s)	1114
AoxI	GGCC	5 cut(s)	30, 143, 476, 659, 794
ApeKI	GCWGC	5 cut(s)	83, 86, 110, 113, 917
ApoI	RAATTY	3 cut(s)	15, 346, 406
Asp700I	GAANNNNTTC	1 cut(s)	1218
AspLEI	GCGC	1 cut(s)	204
AsuC2I	CCSGG	1 cut(s)	267
AsuHPI	GGTGA	1 cut(s)	256
AvaII	GGWCC	7 cut(s)	48, 260, 436, 803, 858, 1090, 1201
AxyI	CCTNAGG	1 cut(s)	1206
BaeGI	GKGCMC	1 cut(s)	1128
BamHI	GGATCC	1 cut(s)	162
BanII	GRGCYC	2 cut(s)	272, 1066
Bbv12I	GWGCWC	1 cut(s)	636
BbvCI	CCTCAGC	1 cut(s)	560
BbvI	GCAGC	5 cut(s)	70, 97, 98, 100, 929
BceAI	ACGGC	1 cut(s)	62
BcgI	CGANNNNNNTGC	6 cut(s)	83, 89, 117, 123, 401, 435
BciVI	GTATCC	1 cut(s)	368
BclI	TGATCA	1 cut(s)	448
BcnI	CCSGG	1 cut(s)	267
BfaI	CTAG	3 cut(s)	455, 927, 975
BfmI	CTRYAG	1 cut(s)	84
BfoI	RGCGCY	1 cut(s)	205
BfuAI	ACCTGC	2 cut(s)	15, 36
BfuI	GTATCC	1 cut(s)	368
BisI	GCNGC	7 cut(s)	75, 84, 87, 111, 114, 192, 918
BlsI	GCNGC	7 cut(s)	76, 85, 88, 112, 115, 193, 919
Bme1390I	CCNGG	1 cut(s)	267
Bme18I	GGWCC	7 cut(s)	48, 260, 436, 803, 858, 1090, 1201
BmiI	GGNNCC	8 cut(s)	164, 437, 660, 795, 804, 859, 1092, 1203
BmrFI	CCNGG	1 cut(s)	267
BmrI	ACTGGG	1 cut(s)	800
BmsI	GCATC	8 cut(s)	91, 403, 614, 634, 676, 698, 1007, 1108
BmuI	ACTGGG	1 cut(s)	800
Bpu10I	CCTNAGC	1 cut(s)	560
BpuEI	CTTGAG	2 cut(s)	372, 1050
BpuMI	CCSGG	1 cut(s)	267
Bsa29I	ATCGAT	1 cut(s)	525
BsaJI	CCNNGG	2 cut(s)	1057, 1225
BsaWI	WCCGGW	1 cut(s)	1114
Bsc4I	CCNNNNNNNGG	1 cut(s)	1121
Bse118I	RCCGGY	1 cut(s)	32
Bse1I	ACTGG	4 cut(s)	246, 423, 795, 1049
Bse21I	CCTNAGG	1 cut(s)	1206
Bse3DI	GCAATG	1 cut(s)	307
BseAI	TCCGGA	1 cut(s)	1114
BseCI	ATCGAT	1 cut(s)	525
BseDI	CCNNGG	2 cut(s)	1057, 1225
BseGI	GGATG	4 cut(s)	625, 691, 1053, 1123
BseLI	CCNNNNNNNGG	1 cut(s)	1121
BseMI	GCAATG	1 cut(s)	307
BseMII	CTCAG	3 cut(s)	501, 574, 1197
BseNI	ACTGG	4 cut(s)	246, 423, 795, 1049
BseSI	GKGCMC	1 cut(s)	1128
BseXI	GCAGC	5 cut(s)	70, 97, 98, 100, 929
BseYI	CCCAGC	1 cut(s)	1121
BshFI	GGCC	5 cut(s)	32, 145, 478, 661, 796
BshVI	ATCGAT	1 cut(s)	525
BsiHKAI	GWGCWC	1 cut(s)	636
BsiSI	CCGG	4 cut(s)	33, 266, 1115, 1183
BslFI	GGGAC	3 cut(s)	871, 966, 1076
BslI	CCNNNNNNNGG	1 cut(s)	1121
BsmFI	GGGAC	3 cut(s)	871, 966, 1076
BsmI	GAATGC	1 cut(s)	583
BsnI	GGCC	5 cut(s)	32, 145, 478, 661, 796
Bsp1286I	GDGCHC	4 cut(s)	272, 636, 1066, 1128
Bsp13I	TCCGGA	1 cut(s)	1114
Bsp143I	GATC	7 cut(s)	162, 183, 254, 290, 448, 467, 1101
BspACI	CCGC	6 cut(s)	51, 75, 116, 192, 465, 861
BspANI	GGCC	5 cut(s)	32, 145, 478, 661, 796
BspCNI	CTCAG	3 cut(s)	502, 573, 1198
BspDI	ATCGAT	1 cut(s)	525
BspEI	TCCGGA	1 cut(s)	1114
BspLI	GGNNCC	8 cut(s)	164, 437, 660, 795, 804, 859, 1092, 1203
BspMAI	CTGCAG	1 cut(s)	88
BspMI	ACCTGC	2 cut(s)	15, 36
BspPI	GGATC	5 cut(s)	157, 170, 262, 285, 475
BsrDI	GCAATG	1 cut(s)	307
BsrFI	RCCGGY	1 cut(s)	32
BsrI	ACTGG	4 cut(s)	246, 423, 795, 1049
BssAI	RCCGGY	1 cut(s)	32
BssECI	CCNNGG	2 cut(s)	1057, 1225
BssMI	GATC	7 cut(s)	162, 183, 254, 290, 448, 467, 1101
BssT1I	CCWWGG	2 cut(s)	1057, 1225
Bst4CI	ACNGT	2 cut(s)	325, 380
Bst6I	CTCTTC	1 cut(s)	867
BstC8I	GCNNGC	5 cut(s)	34, 206, 476, 1075, 1180
BstDEI	CTNAG	3 cut(s)	510, 560, 1206
BstF5I	GGATG	4 cut(s)	625, 691, 1053, 1123
BstH2I	RGCGCY	1 cut(s)	205
BstHHI	GCGC	1 cut(s)	204
BstKTI	GATC	7 cut(s)	165, 186, 257, 293, 451, 470, 1104
BstMBI	GATC	7 cut(s)	162, 183, 254, 290, 448, 467, 1101
BstMWI	GCNNNNNNNGC	9 cut(s)	42, 80, 83, 110, 471, 631, 640, 923, 1070
BstNSI	RCATGY	1 cut(s)	492
BstSCI	CCNGG	1 cut(s)	265
BstSFI	CTRYAG	1 cut(s)	84
BstSLI	GKGCMC	1 cut(s)	1128
BstV1I	GCAGC	5 cut(s)	70, 97, 98, 100, 929
BstX2I	RGATCY	2 cut(s)	162, 254
BstYI	RGATCY	2 cut(s)	162, 254
Bsu15I	ATCGAT	1 cut(s)	525
Bsu36I	CCTNAGG	1 cut(s)	1206
BsuI	GTATCC	1 cut(s)	368
BsuRI	GGCC	5 cut(s)	32, 145, 478, 661, 796
BsuTUI	ATCGAT	1 cut(s)	525
BtgZI	GCGATG	1 cut(s)	172
BtsCI	GGATG	4 cut(s)	625, 691, 1053, 1123
BtsIMutI	CAGTG	1 cut(s)	498
BveI	ACCTGC	2 cut(s)	15, 36
Cac8I	GCNNGC	5 cut(s)	34, 206, 476, 1075, 1180
CfoI	GCGC	1 cut(s)	204
Cfr10I	RCCGGY	1 cut(s)	32
ClaI	ATCGAT	1 cut(s)	525
Csp6I	GTAC	3 cut(s)	613, 904, 949
CspCI	CAANNNNNGTGG	2 cut(s)	910, 945
CviAII	CATG	4 cut(s)	489, 516, 723, 1095
CviQI	GTAC	3 cut(s)	613, 904, 949
DdeI	CTNAG	3 cut(s)	510, 560, 1206
DpnI	GATC	7 cut(s)	164, 185, 256, 292, 450, 469, 1103
DpnII	GATC	7 cut(s)	162, 183, 254, 290, 448, 467, 1101
EaeI	YGGCCR	1 cut(s)	30
Eam1104I	CTCTTC	1 cut(s)	867
EarI	CTCTTC	1 cut(s)	867
EciI	GGCGGA	2 cut(s)	40, 480
Eco130I	CCWWGG	2 cut(s)	1057, 1225
Eco147I	AGGCCT	1 cut(s)	478
Eco24I	GRGCYC	2 cut(s)	272, 1066
Eco32I	GATATC	1 cut(s)	1037
Eco47I	GGWCC	7 cut(s)	48, 260, 436, 803, 858, 1090, 1201
Eco57I	CTGAAG	3 cut(s)	522, 641, 891
Eco81I	CCTNAGG	1 cut(s)	1206
EcoO109I	RGGNCCY	5 cut(s)	436, 659, 794, 1090, 1201
EcoRI	GAATTC	1 cut(s)	346
EcoRV	GATATC	1 cut(s)	1037
EcoT14I	CCWWGG	2 cut(s)	1057, 1225
EcoT22I	ATGCAT	1 cut(s)	691
EcoT38I	GRGCYC	2 cut(s)	272, 1066
ErhI	CCWWGG	2 cut(s)	1057, 1225
FaeI	CATG	4 cut(s)	492, 519, 726, 1098
FaqI	GGGAC	3 cut(s)	871, 966, 1076
FatI	CATG	4 cut(s)	488, 515, 722, 1094
FbaI	TGATCA	1 cut(s)	448
Fnu4HI	GCNGC	7 cut(s)	75, 84, 87, 111, 114, 192, 918
FokI	GGATG	4 cut(s)	612, 698, 1060, 1130
FriOI	GRGCYC	2 cut(s)	272, 1066
Fsp4HI	GCNGC	7 cut(s)	75, 84, 87, 111, 114, 192, 918
FspBI	CTAG	3 cut(s)	455, 927, 975
GlaI	GCGC	1 cut(s)	203
GluI	GCNGC	7 cut(s)	75, 84, 87, 111, 114, 192, 918
GsaI	CCCAGC	1 cut(s)	1125
HaeII	RGCGCY	1 cut(s)	205
HaeIII	GGCC	5 cut(s)	32, 145, 478, 661, 796
HapII	CCGG	4 cut(s)	33, 266, 1115, 1183
HhaI	GCGC	1 cut(s)	204
Hin1II	CATG	4 cut(s)	492, 519, 726, 1098
Hin6I	GCGC	1 cut(s)	202
HinP1I	GCGC	1 cut(s)	202
HpaII	CCGG	4 cut(s)	33, 266, 1115, 1183
HphI	GGTGA	1 cut(s)	256
Hpy166II	GTNNAC	1 cut(s)	904
Hpy188I	TCNGA	8 cut(s)	100, 295, 621, 685, 785, 819, 965, 1003
Hpy188III	TCNNGA	9 cut(s)	24, 220, 236, 258, 629, 882, 1040, 1067, 1115
Hpy8I	GTNNAC	1 cut(s)	904
Hpy99I	CGWCG	1 cut(s)	25
HpyAV	CCTTC	1 cut(s)	1081
HpyCH4III	ACNGT	2 cut(s)	325, 380
HpyCH4IV	ACGT	1 cut(s)	636
HpyCH4V	TGCA	4 cut(s)	86, 605, 689, 998
HpyF10VI	GCNNNNNNNGC	9 cut(s)	42, 80, 83, 110, 471, 631, 640, 923, 1070
HpyF3I	CTNAG	3 cut(s)	510, 560, 1206
HpySE526I	ACGT	1 cut(s)	636
Hsp92II	CATG	4 cut(s)	492, 519, 726, 1098
HspAI	GCGC	1 cut(s)	202
Kpn2I	TCCGGA	1 cut(s)	1114
KroI	GCCGGC	1 cut(s)	32
KroNI	GCCGGC	1 cut(s)	34
Ksp22I	TGATCA	1 cut(s)	448
Kzo9I	GATC	7 cut(s)	162, 183, 254, 290, 448, 467, 1101
LmnI	GCTCC	3 cut(s)	731, 934, 965
Lsp1109I	GCAGC	5 cut(s)	70, 97, 98, 100, 929
LweI	GCATC	8 cut(s)	91, 403, 614, 634, 676, 698, 1007, 1108
MaeI	CTAG	3 cut(s)	455, 927, 975
MaeII	ACGT	1 cut(s)	636
MalI	GATC	7 cut(s)	164, 185, 256, 292, 450, 469, 1103
MboI	GATC	7 cut(s)	162, 183, 254, 290, 448, 467, 1101
MboII	GAAGA	4 cut(s)	317, 388, 515, 884
MflI	RGATCY	2 cut(s)	162, 254
MhlI	GDGCHC	4 cut(s)	272, 636, 1066, 1128
MluCI	AATT	7 cut(s)	15, 39, 346, 406, 710, 735, 760
MlyI	GAGTC	4 cut(s)	217, 689, 764, 988
MmeI	TCCRAC	2 cut(s)	140, 663
Mph1103I	ATGCAT	1 cut(s)	691
MroI	TCCGGA	1 cut(s)	1114
MroNI	GCCGGC	1 cut(s)	32
MroXI	GAANNNNTTC	1 cut(s)	1218
MseI	TTAA	4 cut(s)	120, 639, 705, 944
MslI	CAYNNNNRTG	1 cut(s)	1003
MspA1I	CMGCKG	1 cut(s)	1166
MspI	CCGG	4 cut(s)	33, 266, 1115, 1183
MspR9I	CCNGG	1 cut(s)	267
Mva1269I	GAATGC	1 cut(s)	583
MwoI	GCNNNNNNNGC	9 cut(s)	42, 80, 83, 110, 471, 631, 640, 923, 1070
NaeI	GCCGGC	1 cut(s)	34
NciI	CCSGG	1 cut(s)	267
NdeII	GATC	7 cut(s)	162, 183, 254, 290, 448, 467, 1101
NgoMIV	GCCGGC	1 cut(s)	32
NlaIII	CATG	4 cut(s)	492, 519, 726, 1098
NlaIV	GGNNCC	8 cut(s)	164, 437, 660, 795, 804, 859, 1092, 1203
NmeAIII	GCCGAG	2 cut(s)	79, 220
NsiI	ATGCAT	1 cut(s)	691
NspI	RCATGY	1 cut(s)	492
PceI	AGGCCT	1 cut(s)	478
PciI	ACATGT	1 cut(s)	488
PcsI	WCGNNNNNNNCGW	1 cut(s)	689
PctI	GAATGC	1 cut(s)	583
PdiI	GCCGGC	1 cut(s)	34
PdmI	GAANNNNTTC	1 cut(s)	1218
PfeI	GAWTC	7 cut(s)	95, 367, 527, 878, 1111, 1155, 1214
PkrI	GCNGC	7 cut(s)	76, 85, 88, 112, 115, 193, 919
PleI	GAGTC	4 cut(s)	217, 688, 763, 987
PpsI	GAGTC	4 cut(s)	217, 688, 763, 987
PpuMI	RGGWCCY	3 cut(s)	436, 1090, 1201
PscI	ACATGT	1 cut(s)	488
Psp5II	RGGWCCY	3 cut(s)	436, 1090, 1201
PspFI	CCCAGC	1 cut(s)	1121
PspN4I	GGNNCC	8 cut(s)	164, 437, 660, 795, 804, 859, 1092, 1203
PspPPI	RGGWCCY	3 cut(s)	436, 1090, 1201
PstI	CTGCAG	1 cut(s)	88
PsuI	RGATCY	2 cut(s)	162, 254
PvuII	CAGCTG	1 cut(s)	1166
RsaI	GTAC	3 cut(s)	614, 905, 950
RsaNI	GTAC	3 cut(s)	613, 904, 949
RseI	CAYNNNNRTG	1 cut(s)	1003
SaqAI	TTAA	4 cut(s)	120, 639, 705, 944
SatI	GCNGC	7 cut(s)	75, 84, 87, 111, 114, 192, 918
Sau3AI	GATC	7 cut(s)	162, 183, 254, 290, 448, 467, 1101
SchI	GAGTC	4 cut(s)	217, 689, 764, 988
ScrFI	CCNGG	1 cut(s)	267
SduI	GDGCHC	4 cut(s)	272, 636, 1066, 1128
SfaNI	GCATC	8 cut(s)	91, 403, 614, 634, 676, 698, 1007, 1108
SfcI	CTRYAG	1 cut(s)	84
SinI	GGWCC	7 cut(s)	48, 260, 436, 803, 858, 1090, 1201
SmiMI	CAYNNNNRTG	1 cut(s)	1003
SmlI	CTYRAG	2 cut(s)	387, 1065
SmoI	CTYRAG	2 cut(s)	387, 1065
Sse9I	AATT	7 cut(s)	15, 39, 346, 406, 710, 735, 760
SseBI	AGGCCT	1 cut(s)	478
SsiI	CCGC	6 cut(s)	51, 75, 116, 192, 465, 861
SspMI	CTAG	3 cut(s)	455, 927, 975
StuI	AGGCCT	1 cut(s)	478
StyD4I	CCNGG	1 cut(s)	265
StyI	CCWWGG	2 cut(s)	1057, 1225
TaaI	ACNGT	2 cut(s)	325, 380
TaiI	ACGT	1 cut(s)	639
TaqI	TCGA	4 cut(s)	23, 289, 411, 525
TasI	AATT	7 cut(s)	15, 39, 346, 406, 710, 735, 760
TatI	WGTACW	1 cut(s)	948
TauI	GCSGC	2 cut(s)	77, 194
TfiI	GAWTC	7 cut(s)	95, 367, 527, 878, 1111, 1155, 1214
Tru1I	TTAA	4 cut(s)	120, 639, 705, 944
Tru9I	TTAA	4 cut(s)	120, 639, 705, 944
TscAI	CASTG	1 cut(s)	505
TseI	GCWGC	5 cut(s)	83, 86, 110, 113, 917
TspDTI	ATGAA	8 cut(s)	17, 318, 318, 511, 663, 1064, 1100, 1126
TspGWI	ACGGA	3 cut(s)	146, 387, 1047
TspRI	CASTG	1 cut(s)	505
VpaK11BI	GGWCC	7 cut(s)	48, 260, 436, 803, 858, 1090, 1201
XapI	RAATTY	3 cut(s)	15, 346, 406
XceI	RCATGY	1 cut(s)	492
XmnI	GAANNNNTTC	1 cut(s)	1218
XspI	CTAG	3 cut(s)	455, 927, 975
Zsp2I	ATGCAT	1 cut(s)	691

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.