FvH4_2g39310

Transmembrane protein 136

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0007728
Physical Location & Seq
Reverse (-)
28265135 .. 28266870
1736 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g39310.t1

Sequence Viewer

Length: 711 bp
ATGGAAGAGCATGGAGTTACAAGCCTTGTAGTTCTGGGAGTTATTTCATGGACAACAGCATTTTTATTCATCAGAAAAATGCTTCCAAAGAGATCATTTGTATTCTGCAATCGCCTTGTTTCGACAATTCATGCAACCATTGCTGTAACCCTAGCTTCGCTCTCCGTCGAAGACTGGAGGTGCCCAGTTTGTCCTCTGGCTGCTCAATCTTCTCCTCAGCAGATGAAAGCACTAGCAGTGAGTCTTTCTTATCTCATCTATGATCTCATATGTTGCCTCTTTGACAAGCAATTCAGTCTTGACAATGTCTTCCACCATCTGGTGAGCATTGTTGGAATTGGAGCAGGCCTTTCATATCAAAAGTGTGGGACAGAGATGGTAGCAGCACTGTGGATAACAGAGGTCTCTAGCCCTTTCCTCCACTTGAGGGAGATTCTCAAAGAGCTTGGTTACAGGGACACCGACCTAAACTTAGCAGCAGATATTTCATTTGCAGCTATATTCTCGGTTGCAAGGATGGGAGGCGGACCTTATCTTACATACGTAACACTATCTGCTCACAATCCTTTCCTCATTAAGGGAATGGCAGTTGGTCTGCAGCTGGTCAGTACTTTCTGGTTCTACAAGATTGCGAGAATGGTGATATACAAATTGACCAAGAGGACTAGTACTGCAAAGGAGGCTGATATCAACCATAAAAGTGAAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TRAM_LAG1_CLN8

Protein Analysis

237

Amino Acids

26.24

Weight (kDa)

8.71

Isoelectric Point (pI)

38.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TRAM_LAG1_CLN8 PF03798 35 - 212 8.3e-24 TLC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 180
AccB7I CCANNNNNTGG 1 cut(s) 319
AciI CCGC 1 cut(s) 525
AfaI GTAC 2 cut(s) 610, 670
AfiI CCNNNNNNNGG 3 cut(s) 319, 427, 577
AhlI ACTAGT 1 cut(s) 665
AluBI AGCT 4 cut(s) 155, 445, 497, 601
AluI AGCT 4 cut(s) 155, 445, 497, 601
Alw26I GTCTC 1 cut(s) 409
AoxI GGCC 1 cut(s) 346
ApeKI GCWGC 5 cut(s) 200, 383, 476, 494, 598
AspS9I GGNCC 1 cut(s) 527
AsuHPI GGTGA 2 cut(s) 334, 652
AvaII GGWCC 1 cut(s) 527
BaeGI GKGCMC 1 cut(s) 185
BanI GGYRCC 1 cut(s) 180
BbsI GAAGAC 2 cut(s) 177, 301
BbvCI CCTCAGC 1 cut(s) 216
BbvI GCAGC 5 cut(s) 187, 395, 488, 506, 610
BccI CCATC 3 cut(s) 324, 370, 511
BcoDI GTCTC 1 cut(s) 409
BcuI ACTAGT 1 cut(s) 665
BfaI CTAG 4 cut(s) 152, 233, 408, 666
BfmI CTRYAG 1 cut(s) 596
BisI GCNGC 5 cut(s) 201, 384, 477, 495, 599
BlsI GCNGC 5 cut(s) 202, 385, 478, 496, 600
BmcAI AGTACT 2 cut(s) 610, 670
Bme18I GGWCC 1 cut(s) 527
BmgT120I GGNCC 1 cut(s) 527
BmiI GGNNCC 1 cut(s) 182
BmrI ACTGGG 1 cut(s) 179
BmuI ACTGGG 1 cut(s) 179
BpiI GAAGAC 2 cut(s) 177, 301
BpmI CTGGAG 1 cut(s) 196
Bpu10I CCTNAGC 1 cut(s) 216
BpuEI CTTGAG 1 cut(s) 445
BsaAI YACGTR 1 cut(s) 544
BsaI GGTCTC 1 cut(s) 409
Bsc4I CCNNNNNNNGG 3 cut(s) 319, 427, 577
Bse1I ACTGG 2 cut(s) 179, 185
Bse3DI GCAATG 1 cut(s) 138
BseGI GGATG 1 cut(s) 522
BseLI CCNNNNNNNGG 3 cut(s) 319, 427, 577
BseMI GCAATG 1 cut(s) 138
BseMII CTCAG 1 cut(s) 230
BseNI ACTGG 2 cut(s) 179, 185
BseRI GAGGAG 1 cut(s) 204
BseSI GKGCMC 1 cut(s) 185
BseXI GCAGC 5 cut(s) 187, 395, 488, 506, 610
BshFI GGCC 1 cut(s) 348
BshNI GGYRCC 1 cut(s) 180
BslFI GGGAC 2 cut(s) 382, 470
BslI CCNNNNNNNGG 3 cut(s) 319, 427, 577
BsmAI GTCTC 1 cut(s) 409
BsmFI GGGAC 2 cut(s) 382, 470
BsnI GGCC 1 cut(s) 348
Bso31I GGTCTC 1 cut(s) 409
Bsp1286I GDGCHC 1 cut(s) 185
Bsp143I GATC 2 cut(s) 92, 262
BspACI CCGC 1 cut(s) 525
BspANI GGCC 1 cut(s) 348
BspCNI CTCAG 1 cut(s) 229
BspLI GGNNCC 1 cut(s) 182
BspMAI CTGCAG 1 cut(s) 600
BspT107I GGYRCC 1 cut(s) 180
BspTNI GGTCTC 1 cut(s) 409
BsrDI GCAATG 1 cut(s) 138
BsrI ACTGG 2 cut(s) 179, 185
BssMI GATC 2 cut(s) 92, 262
Bst4CI ACNGT 1 cut(s) 390
BstAPI GCANNNNNTGC 1 cut(s) 140
BstBAI YACGTR 1 cut(s) 544
BstC8I GCNNGC 1 cut(s) 346
BstDEI CTNAG 2 cut(s) 216, 472
BstENI CCTNNNNNAGG 1 cut(s) 575
BstF5I GGATG 1 cut(s) 522
BstKTI GATC 2 cut(s) 95, 265
BstMAI GTCTC 1 cut(s) 409
BstMBI GATC 2 cut(s) 92, 262
BstMWI GCNNNNNNNGC 2 cut(s) 140, 680
BstSFI CTRYAG 1 cut(s) 596
BstSLI GKGCMC 1 cut(s) 185
BstSNI TACGTA 1 cut(s) 544
BstV1I GCAGC 5 cut(s) 187, 395, 488, 506, 610
BstV2I GAAGAC 2 cut(s) 177, 301
BsuRI GGCC 1 cut(s) 348
BtsCI GGATG 1 cut(s) 522
BtsI GCAGTG 1 cut(s) 243
BtsIMutI CAGTG 2 cut(s) 243, 386
Cac8I GCNNGC 1 cut(s) 346
Cfr13I GGNCC 1 cut(s) 527
Csp6I GTAC 2 cut(s) 609, 669
CviAII CATG 3 cut(s) 11, 48, 131
CviJI RGCY 9 cut(s) 24, 155, 200, 348, 411, 445, 497, 601, 683
CviKI_1 RGCY 9 cut(s) 24, 155, 200, 348, 411, 445, 497, 601, 683
CviQI GTAC 2 cut(s) 609, 669
DdeI CTNAG 2 cut(s) 216, 472
DpnI GATC 2 cut(s) 94, 264
DpnII GATC 2 cut(s) 92, 262
EciI GGCGGA 1 cut(s) 540
Eco105I TACGTA 1 cut(s) 544
Eco147I AGGCCT 1 cut(s) 348
Eco31I GGTCTC 1 cut(s) 409
Eco32I GATATC 1 cut(s) 688
Eco47I GGWCC 1 cut(s) 527
EcoNI CCTNNNNNAGG 1 cut(s) 575
EcoRV GATATC 1 cut(s) 688
FaeI CATG 3 cut(s) 14, 51, 134
FaqI GGGAC 2 cut(s) 382, 470
FatI CATG 3 cut(s) 10, 47, 130
FauNDI CATATG 1 cut(s) 269
Fnu4HI GCNGC 5 cut(s) 201, 384, 477, 495, 599
FokI GGATG 1 cut(s) 529
Fsp4HI GCNGC 5 cut(s) 201, 384, 477, 495, 599
FspBI CTAG 4 cut(s) 152, 233, 408, 666
GluI GCNGC 5 cut(s) 201, 384, 477, 495, 599
GsuI CTGGAG 1 cut(s) 196
HaeIII GGCC 1 cut(s) 348
Hin1II CATG 3 cut(s) 14, 51, 134
HinfI GANTC 2 cut(s) 241, 433
HphI GGTGA 2 cut(s) 334, 652
Hpy188I TCNGA 1 cut(s) 74
Hpy188III TCNNGA 1 cut(s) 299
Hpy99I CGWCG 1 cut(s) 170
HpyCH4III ACNGT 1 cut(s) 390
HpyCH4IV ACGT 1 cut(s) 543
HpyCH4V TGCA 6 cut(s) 108, 134, 494, 512, 598, 674
HpyF10VI GCNNNNNNNGC 2 cut(s) 140, 680
HpyF3I CTNAG 2 cut(s) 216, 472
HpySE526I ACGT 1 cut(s) 543
Hsp92II CATG 3 cut(s) 14, 51, 134
Kzo9I GATC 2 cut(s) 92, 262
LmnI GCTCC 1 cut(s) 341
LpnPI CCDG 9 cut(s) 20, 160, 182, 198, 305, 330, 439, 587, 601
Lsp1109I GCAGC 5 cut(s) 187, 395, 488, 506, 610
MaeI CTAG 4 cut(s) 152, 233, 408, 666
MaeII ACGT 1 cut(s) 543
MaeIII GTNAC 4 cut(s) 16, 145, 449, 544
MalI GATC 2 cut(s) 94, 264
MboI GATC 2 cut(s) 92, 262
MboII GAAGA 4 cut(s) 17, 182, 201, 301
MhlI GDGCHC 1 cut(s) 185
MluCI AATT 5 cut(s) 126, 290, 336, 650, 706
MlyI GAGTC 1 cut(s) 250
MmeI TCCRAC 1 cut(s) 313
MseI TTAA 1 cut(s) 576
MslI CAYNNNNRTG 1 cut(s) 699
MspA1I CMGCKG 1 cut(s) 601
MwoI GCNNNNNNNGC 2 cut(s) 140, 680
NdeI CATATG 1 cut(s) 269
NdeII GATC 2 cut(s) 92, 262
NlaIII CATG 3 cut(s) 14, 51, 134
NlaIV GGNNCC 1 cut(s) 182
PceI AGGCCT 1 cut(s) 348
PfeI GAWTC 1 cut(s) 433
PflFI GACNNNGTC 1 cut(s) 305
PflMI CCANNNNNTGG 1 cut(s) 319
PkrI GCNGC 5 cut(s) 202, 385, 478, 496, 600
PleI GAGTC 1 cut(s) 249
PpsI GAGTC 1 cut(s) 249
Ppu21I YACGTR 1 cut(s) 544
PspN4I GGNNCC 1 cut(s) 182
PspPI GGNCC 1 cut(s) 527
PstI CTGCAG 1 cut(s) 600
PsyI GACNNNGTC 1 cut(s) 305
PvuII CAGCTG 1 cut(s) 601
RsaI GTAC 2 cut(s) 610, 670
RsaNI GTAC 2 cut(s) 609, 669
RseI CAYNNNNRTG 1 cut(s) 699
SaqAI TTAA 1 cut(s) 576
SatI GCNGC 5 cut(s) 201, 384, 477, 495, 599
Sau3AI GATC 2 cut(s) 92, 262
Sau96I GGNCC 1 cut(s) 527
ScaI AGTACT 2 cut(s) 610, 670
SchI GAGTC 1 cut(s) 250
SduI GDGCHC 1 cut(s) 185
SetI ASST 9 cut(s) 157, 182, 405, 447, 468, 499, 532, 546, 603
SfcI CTRYAG 1 cut(s) 596
SinI GGWCC 1 cut(s) 527
SmiMI CAYNNNNRTG 1 cut(s) 699
SmlI CTYRAG 1 cut(s) 424
SmoI CTYRAG 1 cut(s) 424
SnaBI TACGTA 1 cut(s) 544
SpeI ACTAGT 1 cut(s) 665
Sse9I AATT 5 cut(s) 126, 290, 336, 650, 706
SseBI AGGCCT 1 cut(s) 348
SsiI CCGC 1 cut(s) 525
SspMI CTAG 4 cut(s) 152, 233, 408, 666
StuI AGGCCT 1 cut(s) 348
TaaI ACNGT 1 cut(s) 390
TaiI ACGT 1 cut(s) 546
TaqI TCGA 2 cut(s) 122, 168
TasI AATT 5 cut(s) 126, 290, 336, 650, 706
TatI WGTACW 2 cut(s) 608, 668
TfiI GAWTC 1 cut(s) 433
Tru1I TTAA 1 cut(s) 576
Tru9I TTAA 1 cut(s) 576
TscAI CASTG 2 cut(s) 243, 393
TseI GCWGC 5 cut(s) 200, 383, 476, 494, 598
TspDTI ATGAA 6 cut(s) 36, 58, 119, 239, 342, 477
TspGWI ACGGA 1 cut(s) 154
TspRI CASTG 2 cut(s) 243, 393
Tth111I GACNNNGTC 1 cut(s) 305
Van91I CCANNNNNTGG 1 cut(s) 319
VpaK11BI GGWCC 1 cut(s) 527
XagI CCTNNNNNAGG 1 cut(s) 575
XspI CTAG 4 cut(s) 152, 233, 408, 666
ZrmI AGTACT 2 cut(s) 610, 670
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.