Rw6G036400

Transmembrane protein 136

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0007728
Physical Location & Seq
Forward (+)
60893757 .. 60895108
1352 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G036400.1

Sequence Viewer

Length: 717 bp
ATGCATGAAGGGCATGGACTTACAAGTCTTGTGGTATTGGGAGTTATTTCTTGGACAACAGCATTTCTATTCATCAGAAAAATGTGTCCAAAGAGATCCTTTGGATTCTGCAACCGCCTTGTTTCGACAATCCATGCAACCATTGCTATAACCATTGCTTCACTCTCTGTCGAAGACTGGAGATGCCCAGTTTGTCCTCTGGCTGCTCAATCTTCTCCTCAGCAGATGAAAGCACTAGCAGTGAGCCTTTCTTATCTCATCTATGATCTCATCTGTTGCCTTTTTGACAAGCAATTCAGTCTTGACAATTTCTTCCACCATTTGGTTAGCATTATTGGAATTGTAGCAGGCCTTGCATACCAAAGGTGTGGGTCAGAGATGGTAGCAGCATTGTGGATAACAGAGGTCTCTAGCCCTTTCCTCCACTTGAGGGAGATTCTCAAAGAGCTTGGTTACAAGGACACCGACCTAAACTTAGCAGCAGATATTTCATTTGCAGCTATATTCTCGGTTGCAAGGATGGGAGGCGGACCTTATCTCACTTATGTAACACTATCTGCTCACAATCCTTTCATCATTAAGGGAATGGCAGTGGGACTGCAGCTGGTCAGTACTTTCTGGTTCTACAAGATAGCGAGAATGGTGATGTACAAATTGACCAAGAGGACTACTAGTGCAATGAAGGAGGCTGACATGAACCATAAAAGAGAAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TRAM_LAG1_CLN8

Protein Analysis

238

Amino Acids

26.59

Weight (kDa)

8.96

Isoelectric Point (pI)

43.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TRAM_LAG1_CLN8 PF03798 36 - 213 2.8e-24 TLC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 24
AccB7I CCANNNNNTGG 1 cut(s) 322
AciI CCGC 2 cut(s) 115, 528
AclWI GGATC 1 cut(s) 90
AfaI GTAC 2 cut(s) 613, 650
AfiI CCNNNNNNNGG 2 cut(s) 322, 430
AhlI ACTAGT 1 cut(s) 671
AluBI AGCT 3 cut(s) 448, 500, 604
AluI AGCT 3 cut(s) 448, 500, 604
Alw26I GTCTC 1 cut(s) 412
AlwI GGATC 1 cut(s) 90
AoxI GGCC 1 cut(s) 349
ApeKI GCWGC 5 cut(s) 203, 386, 479, 497, 601
AspS9I GGNCC 1 cut(s) 530
AsuHPI GGTGA 1 cut(s) 655
AvaII GGWCC 1 cut(s) 530
BbsI GAAGAC 1 cut(s) 180
BbvCI CCTCAGC 1 cut(s) 219
BbvI GCAGC 5 cut(s) 190, 398, 491, 509, 613
BccI CCATC 2 cut(s) 373, 514
BcoDI GTCTC 1 cut(s) 412
BcuI ACTAGT 1 cut(s) 671
BfaI CTAG 3 cut(s) 236, 411, 672
BfmI CTRYAG 1 cut(s) 599
BisI GCNGC 5 cut(s) 204, 387, 480, 498, 602
BlsI GCNGC 5 cut(s) 205, 388, 481, 499, 603
BmcAI AGTACT 1 cut(s) 613
Bme18I GGWCC 1 cut(s) 530
BmgT120I GGNCC 1 cut(s) 530
BmrI ACTGGG 1 cut(s) 182
BmsI GCATC 1 cut(s) 173
BmuI ACTGGG 1 cut(s) 182
BpiI GAAGAC 1 cut(s) 180
BpmI CTGGAG 1 cut(s) 199
Bpu10I CCTNAGC 1 cut(s) 219
BpuEI CTTGAG 1 cut(s) 448
BsaI GGTCTC 1 cut(s) 412
Bsc4I CCNNNNNNNGG 2 cut(s) 322, 430
Bse1I ACTGG 2 cut(s) 182, 188
Bse3DI GCAATG 3 cut(s) 141, 153, 684
BseGI GGATG 1 cut(s) 525
BseLI CCNNNNNNNGG 2 cut(s) 322, 430
BseMI GCAATG 3 cut(s) 141, 153, 684
BseMII CTCAG 1 cut(s) 233
BseNI ACTGG 2 cut(s) 182, 188
BseRI GAGGAG 1 cut(s) 207
BseXI GCAGC 5 cut(s) 190, 398, 491, 509, 613
BshFI GGCC 1 cut(s) 351
BslFI GGGAC 1 cut(s) 609
BslI CCNNNNNNNGG 2 cut(s) 322, 430
BsmAI GTCTC 1 cut(s) 412
BsmFI GGGAC 1 cut(s) 609
BsnI GGCC 1 cut(s) 351
Bso31I GGTCTC 1 cut(s) 412
Bsp1407I TGTACA 1 cut(s) 648
Bsp143I GATC 2 cut(s) 95, 265
BspACI CCGC 2 cut(s) 115, 528
BspANI GGCC 1 cut(s) 351
BspCNI CTCAG 1 cut(s) 232
BspMAI CTGCAG 1 cut(s) 603
BspPI GGATC 1 cut(s) 90
BspTNI GGTCTC 1 cut(s) 412
BsrDI GCAATG 3 cut(s) 141, 153, 684
BsrGI TGTACA 1 cut(s) 648
BsrI ACTGG 2 cut(s) 182, 188
BssMI GATC 2 cut(s) 95, 265
BstAPI GCANNNNNTGC 2 cut(s) 143, 353
BstAUI TGTACA 1 cut(s) 648
BstC8I GCNNGC 1 cut(s) 349
BstDEI CTNAG 2 cut(s) 219, 475
BstF5I GGATG 1 cut(s) 525
BstKTI GATC 2 cut(s) 98, 268
BstMAI GTCTC 1 cut(s) 412
BstMBI GATC 2 cut(s) 95, 265
BstMWI GCNNNNNNNGC 3 cut(s) 10, 143, 353
BstSFI CTRYAG 1 cut(s) 599
BstV1I GCAGC 5 cut(s) 190, 398, 491, 509, 613
BstV2I GAAGAC 1 cut(s) 180
BstX2I RGATCY 1 cut(s) 95
BstXI CCANNNNNNTGG 1 cut(s) 368
BstYI RGATCY 1 cut(s) 95
BsuRI GGCC 1 cut(s) 351
BtsCI GGATG 1 cut(s) 525
BtsI GCAGTG 2 cut(s) 246, 597
BtsIMutI CAGTG 2 cut(s) 246, 597
Cac8I GCNNGC 1 cut(s) 349
Cfr13I GGNCC 1 cut(s) 530
Csp6I GTAC 2 cut(s) 612, 649
CspCI CAANNNNNGTGG 2 cut(s) 12, 47
CviAII CATG 4 cut(s) 5, 14, 134, 694
CviJI RGCY 8 cut(s) 203, 246, 351, 414, 448, 500, 604, 689
CviKI_1 RGCY 8 cut(s) 203, 246, 351, 414, 448, 500, 604, 689
CviQI GTAC 2 cut(s) 612, 649
DdeI CTNAG 2 cut(s) 219, 475
DpnI GATC 2 cut(s) 97, 267
DpnII GATC 2 cut(s) 95, 265
DrdI GACNNNNNNGTC 1 cut(s) 24
DseDI GACNNNNNNGTC 1 cut(s) 24
EciI GGCGGA 1 cut(s) 543
Eco147I AGGCCT 1 cut(s) 351
Eco31I GGTCTC 1 cut(s) 412
Eco47I GGWCC 1 cut(s) 530
EcoT22I ATGCAT 1 cut(s) 6
FaeI CATG 4 cut(s) 8, 17, 137, 697
FalI AAGNNNNNCTT 2 cut(s) 83, 115
FaqI GGGAC 1 cut(s) 609
FatI CATG 4 cut(s) 4, 13, 133, 693
Fnu4HI GCNGC 5 cut(s) 204, 387, 480, 498, 602
FokI GGATG 1 cut(s) 532
Fsp4HI GCNGC 5 cut(s) 204, 387, 480, 498, 602
FspBI CTAG 3 cut(s) 236, 411, 672
GluI GCNGC 5 cut(s) 204, 387, 480, 498, 602
GsuI CTGGAG 1 cut(s) 199
HaeIII GGCC 1 cut(s) 351
Hin1II CATG 4 cut(s) 8, 17, 137, 697
HinfI GANTC 2 cut(s) 105, 436
HphI GGTGA 1 cut(s) 655
Hpy188I TCNGA 2 cut(s) 77, 376
Hpy188III TCNNGA 1 cut(s) 302
HpyAV CCTTC 1 cut(s) 676
HpyCH4V TGCA 8 cut(s) 4, 111, 137, 356, 497, 515, 601, 677
HpyF10VI GCNNNNNNNGC 3 cut(s) 10, 143, 353
HpyF3I CTNAG 2 cut(s) 219, 475
Hsp92II CATG 4 cut(s) 8, 17, 137, 697
Kzo9I GATC 2 cut(s) 95, 265
LpnPI CCDG 6 cut(s) 163, 185, 201, 333, 590, 604
Lsp1109I GCAGC 5 cut(s) 190, 398, 491, 509, 613
LweI GCATC 1 cut(s) 173
MaeI CTAG 3 cut(s) 236, 411, 672
MaeIII GTNAC 2 cut(s) 452, 547
MalI GATC 2 cut(s) 97, 267
MboI GATC 2 cut(s) 95, 265
MboII GAAGA 3 cut(s) 185, 204, 304
MflI RGATCY 1 cut(s) 95
MluCI AATT 5 cut(s) 293, 307, 339, 653, 712
MnlI CCTC 8 cut(s) 207, 228, 397, 423, 431, 518, 657, 679
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 1 cut(s) 579
MspA1I CMGCKG 1 cut(s) 604
MwoI GCNNNNNNNGC 3 cut(s) 10, 143, 353
NdeII GATC 2 cut(s) 95, 265
NlaIII CATG 4 cut(s) 8, 17, 137, 697
NsiI ATGCAT 1 cut(s) 6
PceI AGGCCT 1 cut(s) 351
PfeI GAWTC 2 cut(s) 105, 436
PflMI CCANNNNNTGG 1 cut(s) 322
PkrI GCNGC 5 cut(s) 205, 388, 481, 499, 603
PspPI GGNCC 1 cut(s) 530
PstI CTGCAG 1 cut(s) 603
PsuI RGATCY 1 cut(s) 95
PvuII CAGCTG 1 cut(s) 604
RsaI GTAC 2 cut(s) 613, 650
RsaNI GTAC 2 cut(s) 612, 649
SaqAI TTAA 1 cut(s) 579
SatI GCNGC 5 cut(s) 204, 387, 480, 498, 602
Sau3AI GATC 2 cut(s) 95, 265
Sau96I GGNCC 1 cut(s) 530
ScaI AGTACT 1 cut(s) 613
SetI ASST 7 cut(s) 368, 408, 450, 471, 502, 535, 606
SfaNI GCATC 1 cut(s) 173
SfcI CTRYAG 1 cut(s) 599
SinI GGWCC 1 cut(s) 530
SmlI CTYRAG 1 cut(s) 427
SmoI CTYRAG 1 cut(s) 427
SpeI ACTAGT 1 cut(s) 671
Sse9I AATT 5 cut(s) 293, 307, 339, 653, 712
SseBI AGGCCT 1 cut(s) 351
SsiI CCGC 2 cut(s) 115, 528
SspMI CTAG 3 cut(s) 236, 411, 672
StuI AGGCCT 1 cut(s) 351
TaqI TCGA 2 cut(s) 125, 171
TasI AATT 5 cut(s) 293, 307, 339, 653, 712
TatI WGTACW 2 cut(s) 611, 648
TfiI GAWTC 2 cut(s) 105, 436
Tru1I TTAA 1 cut(s) 579
Tru9I TTAA 1 cut(s) 579
TscAI CASTG 2 cut(s) 246, 597
TseI GCWGC 5 cut(s) 203, 386, 479, 497, 601
TspDTI ATGAA 7 cut(s) 21, 61, 242, 480, 562, 695, 710
TspRI CASTG 2 cut(s) 246, 597
Van91I CCANNNNNTGG 1 cut(s) 322
VpaK11BI GGWCC 1 cut(s) 530
XspI CTAG 3 cut(s) 236, 411, 672
ZrmI AGTACT 1 cut(s) 613
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.