Rh6DG420800

Transmembrane protein 136

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0007728
Physical Location & Seq
Forward (+)
60284772 .. 60287197
2426 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG420800.1

Sequence Viewer

Length: 642 bp
ATGGAAGGGCATGGACTTACAAGTCTTGTAGTATTGGGAGTTATTTCATGGACAACAGCATTTCTATTCATCAGAAAAATGTGTCCAAAGAGATCCTTTGGATTCTGCAACCGCCTTGTTTCGACAATCCATGCAACCATTGCTGTAACCCTAGCTTCACTCTCTGTCGAAGACTGGAGATGCCCAGTTTGTCCTCTGGCTGCTCAATCTTCTCCTCAGCAGATGAAAGCACTAGCAGTGAGCCTTTCTTATCTCATCTATGATCTCATCTGTTGCCTTTTTGACAAGCAATTCAGTCTTGACAATTTCTTCCACCATTTGGTTAGCATTATTGGAATTGTAGCAGGCCTTGCATACCAAAGGTGTGGGTCAGAGATGGTAGCAGCATTGTGGATAACAGAGGTCTCTAGCCCTTTCCTCCACTTGAGGGAGATTCTCAAAGAACTTGGTTACAAGGACACCGACCTAAACTTAGCAGCAGATGTATGCTGTTTGAGAATTCCACAATGTTATAATCTTTCGTATTGTAGCAGAAACAGAATCTACTCTAATATCGTTTTCCATGTTTGTGTGTATAGATTTCATTTGCAGCTATATTCTCGGTTGCAAGGATGGGAGGCGGACCTTATCTCGCTTATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TRAM_LAG1_CLN8

Protein Analysis

213

Amino Acids

24.12

Weight (kDa)

7.97

Isoelectric Point (pI)

42.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TRAM_LAG1_CLN8 PF03798 35 - 158 5.2e-15 TLC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 513
AasI GACNNNNNNGTC 1 cut(s) 21
AccB7I CCANNNNNTGG 1 cut(s) 319
AciI CCGC 2 cut(s) 112, 620
AclWI GGATC 1 cut(s) 87
AcsI RAATTY 1 cut(s) 498
AfiI CCNNNNNNNGG 2 cut(s) 319, 427
AluBI AGCT 2 cut(s) 155, 592
AluI AGCT 2 cut(s) 155, 592
Alw26I GTCTC 1 cut(s) 409
AlwI GGATC 1 cut(s) 87
AoxI GGCC 1 cut(s) 346
ApeKI GCWGC 4 cut(s) 200, 383, 476, 589
ApoI RAATTY 1 cut(s) 498
AspS9I GGNCC 1 cut(s) 622
AvaII GGWCC 1 cut(s) 622
BbsI GAAGAC 1 cut(s) 177
BbvCI CCTCAGC 1 cut(s) 216
BbvI GCAGC 4 cut(s) 187, 395, 488, 601
BccI CCATC 2 cut(s) 370, 606
BcoDI GTCTC 1 cut(s) 409
BfaI CTAG 3 cut(s) 152, 233, 408
BisI GCNGC 4 cut(s) 201, 384, 477, 590
BlsI GCNGC 4 cut(s) 202, 385, 478, 591
Bme18I GGWCC 1 cut(s) 622
BmgT120I GGNCC 1 cut(s) 622
BmrI ACTGGG 1 cut(s) 179
BmsI GCATC 1 cut(s) 170
BmuI ACTGGG 1 cut(s) 179
BpiI GAAGAC 1 cut(s) 177
BpmI CTGGAG 1 cut(s) 196
Bpu10I CCTNAGC 1 cut(s) 216
BpuEI CTTGAG 1 cut(s) 445
BsaI GGTCTC 1 cut(s) 409
Bsc4I CCNNNNNNNGG 2 cut(s) 319, 427
Bse1I ACTGG 2 cut(s) 179, 185
Bse3DI GCAATG 1 cut(s) 138
BseGI GGATG 1 cut(s) 617
BseLI CCNNNNNNNGG 2 cut(s) 319, 427
BseMI GCAATG 1 cut(s) 138
BseMII CTCAG 1 cut(s) 230
BseNI ACTGG 2 cut(s) 179, 185
BseRI GAGGAG 1 cut(s) 204
BseXI GCAGC 4 cut(s) 187, 395, 488, 601
BshFI GGCC 1 cut(s) 348
BslI CCNNNNNNNGG 2 cut(s) 319, 427
BsmAI GTCTC 1 cut(s) 409
BsnI GGCC 1 cut(s) 348
Bso31I GGTCTC 1 cut(s) 409
Bsp143I GATC 2 cut(s) 92, 262
BspACI CCGC 2 cut(s) 112, 620
BspANI GGCC 1 cut(s) 348
BspCNI CTCAG 1 cut(s) 229
BspPI GGATC 1 cut(s) 87
BspTNI GGTCTC 1 cut(s) 409
BsrDI GCAATG 1 cut(s) 138
BsrI ACTGG 2 cut(s) 179, 185
BssMI GATC 2 cut(s) 92, 262
BstAPI GCANNNNNTGC 2 cut(s) 140, 350
BstC8I GCNNGC 1 cut(s) 346
BstDEI CTNAG 2 cut(s) 216, 472
BstF5I GGATG 1 cut(s) 617
BstKTI GATC 2 cut(s) 95, 265
BstMAI GTCTC 1 cut(s) 409
BstMBI GATC 2 cut(s) 92, 262
BstMWI GCNNNNNNNGC 2 cut(s) 140, 350
BstV1I GCAGC 4 cut(s) 187, 395, 488, 601
BstV2I GAAGAC 1 cut(s) 177
BstX2I RGATCY 1 cut(s) 92
BstXI CCANNNNNNTGG 1 cut(s) 365
BstYI RGATCY 1 cut(s) 92
BsuRI GGCC 1 cut(s) 348
BtsCI GGATG 1 cut(s) 617
BtsI GCAGTG 1 cut(s) 243
BtsIMutI CAGTG 1 cut(s) 243
Cac8I GCNNGC 1 cut(s) 346
Cfr13I GGNCC 1 cut(s) 622
CviAII CATG 4 cut(s) 11, 48, 131, 563
CviJI RGCY 6 cut(s) 155, 200, 243, 348, 411, 592
CviKI_1 RGCY 6 cut(s) 155, 200, 243, 348, 411, 592
DdeI CTNAG 2 cut(s) 216, 472
DpnI GATC 2 cut(s) 94, 264
DpnII GATC 2 cut(s) 92, 262
DrdI GACNNNNNNGTC 1 cut(s) 21
DseDI GACNNNNNNGTC 1 cut(s) 21
EciI GGCGGA 1 cut(s) 635
Eco147I AGGCCT 1 cut(s) 348
Eco31I GGTCTC 1 cut(s) 409
Eco47I GGWCC 1 cut(s) 622
EcoRI GAATTC 1 cut(s) 498
FaeI CATG 4 cut(s) 14, 51, 134, 566
FalI AAGNNNNNCTT 2 cut(s) 80, 112
FatI CATG 4 cut(s) 10, 47, 130, 562
Fnu4HI GCNGC 4 cut(s) 201, 384, 477, 590
FokI GGATG 1 cut(s) 624
Fsp4HI GCNGC 4 cut(s) 201, 384, 477, 590
FspBI CTAG 3 cut(s) 152, 233, 408
GluI GCNGC 4 cut(s) 201, 384, 477, 590
GsuI CTGGAG 1 cut(s) 196
HaeIII GGCC 1 cut(s) 348
Hin1II CATG 4 cut(s) 14, 51, 134, 566
HinfI GANTC 3 cut(s) 102, 433, 540
Hpy188I TCNGA 2 cut(s) 74, 373
Hpy188III TCNNGA 1 cut(s) 299
HpyCH4V TGCA 5 cut(s) 108, 134, 353, 589, 607
HpyF10VI GCNNNNNNNGC 2 cut(s) 140, 350
HpyF3I CTNAG 2 cut(s) 216, 472
Hsp92II CATG 4 cut(s) 14, 51, 134, 566
Kzo9I GATC 2 cut(s) 92, 262
LpnPI CCDG 4 cut(s) 160, 182, 198, 330
Lsp1109I GCAGC 4 cut(s) 187, 395, 488, 601
LweI GCATC 1 cut(s) 170
MaeI CTAG 3 cut(s) 152, 233, 408
MaeIII GTNAC 2 cut(s) 145, 449
MalI GATC 2 cut(s) 94, 264
MboI GATC 2 cut(s) 92, 262
MboII GAAGA 3 cut(s) 182, 201, 301
MflI RGATCY 1 cut(s) 92
MluCI AATT 4 cut(s) 290, 304, 336, 498
MnlI CCTC 6 cut(s) 204, 225, 394, 420, 428, 610
MslI CAYNNNNRTG 1 cut(s) 567
MwoI GCNNNNNNNGC 2 cut(s) 140, 350
NdeII GATC 2 cut(s) 92, 262
NlaIII CATG 4 cut(s) 14, 51, 134, 566
PceI AGGCCT 1 cut(s) 348
PfeI GAWTC 3 cut(s) 102, 433, 540
PflMI CCANNNNNTGG 1 cut(s) 319
PkrI GCNGC 4 cut(s) 202, 385, 478, 591
PsiI TTATAA 1 cut(s) 513
PspPI GGNCC 1 cut(s) 622
PsuI RGATCY 1 cut(s) 92
RseI CAYNNNNRTG 1 cut(s) 567
SatI GCNGC 4 cut(s) 201, 384, 477, 590
Sau3AI GATC 2 cut(s) 92, 262
Sau96I GGNCC 1 cut(s) 622
SetI ASST 6 cut(s) 157, 365, 405, 468, 594, 627
SfaNI GCATC 1 cut(s) 170
SinI GGWCC 1 cut(s) 622
SmiMI CAYNNNNRTG 1 cut(s) 567
SmlI CTYRAG 1 cut(s) 424
SmoI CTYRAG 1 cut(s) 424
Sse9I AATT 4 cut(s) 290, 304, 336, 498
SseBI AGGCCT 1 cut(s) 348
SsiI CCGC 2 cut(s) 112, 620
SspMI CTAG 3 cut(s) 152, 233, 408
StuI AGGCCT 1 cut(s) 348
TaqI TCGA 2 cut(s) 122, 168
TasI AATT 4 cut(s) 290, 304, 336, 498
TfiI GAWTC 3 cut(s) 102, 433, 540
TscAI CASTG 1 cut(s) 243
TseI GCWGC 4 cut(s) 200, 383, 476, 589
TspDTI ATGAA 4 cut(s) 36, 58, 239, 572
TspRI CASTG 1 cut(s) 243
Van91I CCANNNNNTGG 1 cut(s) 319
VpaK11BI GGWCC 1 cut(s) 622
XapI RAATTY 1 cut(s) 498
XspI CTAG 3 cut(s) 152, 233, 408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.