MD08G1024400.v1.1

Transmembrane protein 136

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0007728
Physical Location & Seq
Reverse (-)
1760134 .. 1762274
2141 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1024400.v1.1.491

Sequence Viewer

Length: 726 bp
ATGGAGGAGCAAATTGCAAGTATGGTGGTGTTGGGGGTGATTTCATGGACTACGGCGTTTCTGTTCATACGAAAGATGCTTCCGAAGCGATCGTTCGACTTCTGCAACCGCCTTGTATCCACAGTTCATGCAACTGTGGCTGTTACCTTAGCTTCCCTTTCTGTTCAAGATTGGAACTGCCCTGTGTGCCCTCTGGCTTCAACTTCTTCTCCTCGTCAGATGAAAGCACTGTCTTTGAGCCTTTCTTATCTCATATACGATCTTGTGTGTTGCCTCTTTGACAAGAAATTCAATCCTGATAATTTCTTCCACCATTTGGTCAGCATTGTTGGCATTGGAGCAGGCCTTTCCTACCAAAAGTGTGGGTCGGAGATGGTGGCAGCATTGTGGATAACAGAGTTGTCCAGCCCTTTCCTACATATAAGGGAGCTTCTCAAAGAGCTTGGTTACAGAGACTCCGATCTAAACCTAGCAGCCGATATTTTATTTGCAGTTATATTCACGGTTGCAAGGATGGGAGGCGGGCCTTATCTCACCTATGTGACTCTGTCTGCTAACAACCCATTCATCATCAAGGCGATGGCGGTAGGGTTGCAGCTGGTGAGCGCTTTCTGGTTCTACAAGATTGCGAGGATGGTCAAGTACAAATTGACAAAGAGGACGACTACTGCTGCTGCATCTAGTAAACAAGTCGATCCCCCAACCCATAAAACACGTAGCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TRAM_LAG1_CLN8

Protein Analysis

242

Amino Acids

26.79

Weight (kDa)

9.33

Isoelectric Point (pI)

39.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TRAM_LAG1_CLN8 PF03798 33 - 211 4.1e-26 TLC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 316
AciI CCGC 3 cut(s) 109, 522, 584
AclWI GGATC 1 cut(s) 689
AcsI RAATTY 1 cut(s) 287
AfaI GTAC 1 cut(s) 644
AfeI AGCGCT 1 cut(s) 607
AfiI CCNNNNNNNGG 1 cut(s) 316
AflIII ACRYGT 1 cut(s) 713
AgsI TTSAA 3 cut(s) 167, 201, 292
AleI CACNNNNGTG 1 cut(s) 539
AluBI AGCT 4 cut(s) 152, 430, 442, 598
AluI AGCT 4 cut(s) 152, 430, 442, 598
Alw26I GTCTC 1 cut(s) 447
AlwI GGATC 1 cut(s) 689
Aor51HI AGCGCT 1 cut(s) 607
AoxI GGCC 2 cut(s) 343, 524
ApeKI GCWGC 5 cut(s) 380, 473, 595, 671, 674
ApoI RAATTY 1 cut(s) 287
AspLEI GCGC 1 cut(s) 608
AspS9I GGNCC 1 cut(s) 524
AsuHPI GGTGA 3 cut(s) 49, 526, 613
BaeGI GKGCMC 1 cut(s) 191
BbvI GCAGC 5 cut(s) 392, 485, 607, 658, 661
BccI CCATC 4 cut(s) 367, 508, 574, 628
BceAI ACGGC 1 cut(s) 69
BciVI GTATCC 1 cut(s) 127
BcoDI GTCTC 1 cut(s) 447
BfaI CTAG 2 cut(s) 470, 681
BfoI RGCGCY 1 cut(s) 609
BfuI GTATCC 1 cut(s) 127
BisI GCNGC 5 cut(s) 381, 474, 596, 672, 675
BlsI GCNGC 5 cut(s) 382, 475, 597, 673, 676
BmgT120I GGNCC 1 cut(s) 524
BmsI GCATC 2 cut(s) 66, 686
Bpu10I CCTNAGC 1 cut(s) 148
BsaAI YACGTR 1 cut(s) 716
BsaXI ACNNNNNCTCC 4 cut(s) 193, 223, 440, 470
Bsc4I CCNNNNNNNGG 1 cut(s) 316
BseGI GGATG 2 cut(s) 519, 639
BseLI CCNNNNNNNGG 1 cut(s) 316
BseRI GAGGAG 2 cut(s) 20, 201
BseSI GKGCMC 1 cut(s) 191
BseXI GCAGC 5 cut(s) 392, 485, 607, 658, 661
Bsh1285I CGRYCG 1 cut(s) 92
BshFI GGCC 2 cut(s) 345, 526
BsiEI CGRYCG 1 cut(s) 92
BslI CCNNNNNNNGG 1 cut(s) 316
BsmAI GTCTC 1 cut(s) 447
BsnI GGCC 2 cut(s) 345, 526
Bsp1286I GDGCHC 1 cut(s) 191
Bsp143I GATC 4 cut(s) 89, 259, 460, 694
BspACI CCGC 3 cut(s) 109, 522, 584
BspANI GGCC 2 cut(s) 345, 526
BspPI GGATC 1 cut(s) 689
BssMI GATC 4 cut(s) 89, 259, 460, 694
Bst4CI ACNGT 4 cut(s) 124, 136, 231, 505
BstBAI YACGTR 1 cut(s) 716
BstC8I GCNNGC 2 cut(s) 343, 524
BstDEI CTNAG 1 cut(s) 148
BstF5I GGATG 2 cut(s) 519, 639
BstH2I RGCGCY 1 cut(s) 609
BstHHI GCGC 1 cut(s) 608
BstKTI GATC 4 cut(s) 92, 262, 463, 697
BstMAI GTCTC 1 cut(s) 447
BstMBI GATC 4 cut(s) 89, 259, 460, 694
BstMCI CGRYCG 1 cut(s) 92
BstMWI GCNNNNNNNGC 4 cut(s) 85, 137, 186, 330
BstSLI GKGCMC 1 cut(s) 191
BstV1I GCAGC 5 cut(s) 392, 485, 607, 658, 661
BstXI CCANNNNNNTGG 1 cut(s) 362
BsuI GTATCC 1 cut(s) 127
BsuRI GGCC 2 cut(s) 345, 526
BtgZI GCGATG 1 cut(s) 593
BtsCI GGATG 2 cut(s) 519, 639
BtsIMutI CAGTG 1 cut(s) 227
Cac8I GCNNGC 2 cut(s) 343, 524
CfoI GCGC 1 cut(s) 608
Cfr13I GGNCC 1 cut(s) 524
Csp6I GTAC 1 cut(s) 643
CspCI CAANNNNNGTGG 2 cut(s) 6, 41
CviAII CATG 2 cut(s) 45, 128
CviQI GTAC 1 cut(s) 643
DdeI CTNAG 1 cut(s) 148
DpnI GATC 4 cut(s) 91, 261, 462, 696
DpnII GATC 4 cut(s) 89, 259, 460, 694
Eco147I AGGCCT 1 cut(s) 345
Eco47III AGCGCT 1 cut(s) 607
FaeI CATG 2 cut(s) 48, 131
FatI CATG 2 cut(s) 44, 127
FauI CCCGC 1 cut(s) 515
Fnu4HI GCNGC 5 cut(s) 381, 474, 596, 672, 675
FokI GGATG 2 cut(s) 526, 646
Fsp4HI GCNGC 5 cut(s) 381, 474, 596, 672, 675
FspBI CTAG 2 cut(s) 470, 681
GlaI GCGC 1 cut(s) 607
GluI GCNGC 5 cut(s) 381, 474, 596, 672, 675
HaeII RGCGCY 1 cut(s) 609
HaeIII GGCC 2 cut(s) 345, 526
HhaI GCGC 1 cut(s) 608
Hin1II CATG 2 cut(s) 48, 131
Hin6I GCGC 1 cut(s) 606
HinP1I GCGC 1 cut(s) 606
HinfI GANTC 2 cut(s) 455, 544
HphI GGTGA 3 cut(s) 49, 526, 613
Hpy166II GTNNAC 1 cut(s) 686
Hpy188I TCNGA 4 cut(s) 84, 219, 370, 460
Hpy188III TCNNGA 2 cut(s) 167, 296
Hpy8I GTNNAC 1 cut(s) 686
HpyCH4III ACNGT 4 cut(s) 124, 136, 231, 505
HpyCH4IV ACGT 1 cut(s) 715
HpyCH4V TGCA 7 cut(s) 17, 105, 131, 491, 509, 595, 677
HpyF10VI GCNNNNNNNGC 4 cut(s) 85, 137, 186, 330
HpyF3I CTNAG 1 cut(s) 148
HpySE526I ACGT 1 cut(s) 715
Hsp92II CATG 2 cut(s) 48, 131
HspAI GCGC 1 cut(s) 606
Kzo9I GATC 4 cut(s) 89, 259, 460, 694
LmnI GCTCC 3 cut(s) 7, 338, 427
LpnPI CCDG 7 cut(s) 179, 195, 309, 327, 418, 584, 598
Lsp1109I GCAGC 5 cut(s) 392, 485, 607, 658, 661
LweI GCATC 2 cut(s) 66, 686
MaeI CTAG 2 cut(s) 470, 681
MaeII ACGT 1 cut(s) 715
MaeIII GTNAC 3 cut(s) 142, 446, 541
MalI GATC 4 cut(s) 91, 261, 462, 696
MboI GATC 4 cut(s) 89, 259, 460, 694
MboII GAAGA 2 cut(s) 198, 298
MfeI CAATTG 1 cut(s) 721
MhlI GDGCHC 1 cut(s) 191
MluCI AATT 5 cut(s) 12, 287, 301, 647, 721
MlyI GAGTC 2 cut(s) 449, 538
MmeI TCCRAC 1 cut(s) 348
MnlI CCTC 6 cut(s) 201, 222, 284, 512, 624, 651
MslI CAYNNNNRTG 1 cut(s) 539
MspA1I CMGCKG 1 cut(s) 598
MunI CAATTG 1 cut(s) 721
MwoI GCNNNNNNNGC 4 cut(s) 85, 137, 186, 330
NdeII GATC 4 cut(s) 89, 259, 460, 694
NlaIII CATG 2 cut(s) 48, 131
NmuCI GTSAC 1 cut(s) 541
OliI CACNNNNGTG 1 cut(s) 539
PceI AGGCCT 1 cut(s) 345
PflFI GACNNNGTC 1 cut(s) 547
PflMI CCANNNNNTGG 1 cut(s) 316
PkrI GCNGC 5 cut(s) 382, 475, 597, 673, 676
Ple19I CGATCG 1 cut(s) 92
PleI GAGTC 2 cut(s) 449, 538
PpsI GAGTC 2 cut(s) 449, 538
Ppu21I YACGTR 1 cut(s) 716
PspPI GGNCC 1 cut(s) 524
PsrI GAACNNNNNNTAC 2 cut(s) 108, 140
PsyI GACNNNGTC 1 cut(s) 547
PvuI CGATCG 1 cut(s) 92
PvuII CAGCTG 1 cut(s) 598
RsaI GTAC 1 cut(s) 644
RsaNI GTAC 1 cut(s) 643
RseI CAYNNNNRTG 1 cut(s) 539
SatI GCNGC 5 cut(s) 381, 474, 596, 672, 675
Sau3AI GATC 4 cut(s) 89, 259, 460, 694
Sau96I GGNCC 1 cut(s) 524
SchI GAGTC 2 cut(s) 449, 538
SduI GDGCHC 1 cut(s) 191
SetI ASST 8 cut(s) 149, 154, 432, 444, 471, 539, 600, 718
SfaNI GCATC 2 cut(s) 66, 686
SmiMI CAYNNNNRTG 1 cut(s) 539
Sse9I AATT 5 cut(s) 12, 287, 301, 647, 721
SseBI AGGCCT 1 cut(s) 345
SsiI CCGC 3 cut(s) 109, 522, 584
SspMI CTAG 2 cut(s) 470, 681
StuI AGGCCT 1 cut(s) 345
TaaI ACNGT 4 cut(s) 124, 136, 231, 505
TaiI ACGT 1 cut(s) 718
TaqI TCGA 2 cut(s) 96, 693
TasI AATT 5 cut(s) 12, 287, 301, 647, 721
TatI WGTACW 1 cut(s) 642
TscAI CASTG 1 cut(s) 234
TseFI GTSAC 1 cut(s) 541
TseI GCWGC 5 cut(s) 380, 473, 595, 671, 674
Tsp45I GTSAC 1 cut(s) 541
TspDTI ATGAA 5 cut(s) 33, 55, 116, 236, 556
TspRI CASTG 1 cut(s) 234
Tth111I GACNNNGTC 1 cut(s) 547
Van91I CCANNNNNTGG 1 cut(s) 316
XapI RAATTY 1 cut(s) 287
XspI CTAG 2 cut(s) 470, 681
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.