Rmu_sc0005517.1_g000001

Transmembrane protein 136

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005517.1
OG0007728
Physical Location & Seq
Forward (+)
378 .. 1807
1430 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005517.1_g000001.1.cds

Sequence Viewer

Length: 714 bp
atggaagggcatggaattacaagtgttgtagtattgggagttatttcatggacaacagcatttctattcatcagaaaaacgtgtccaaagagatcctttggattctgcaaccgccttgtttcgacaatccatgcaaccattgctataaccattgcttcactctctgtcgaagactggagatgcccagtttgtcctctggctgctcaatcttctcctcagcagatgaaagcactagcagtgagcctttcttatctcatctatgatctcatctgttgcctttttgacaagcaattcagtcttgacaatttcttccaccatttggttagcattattggaattgtagcaggccttgcataccaaaggtgtgggtcagagatggtagcagcattgtggataacagaggtctctagccccttcctccacttgagggagattctcaaagagcttggttacaaggacaccgatctaaacttagcagcagatatttcatttgcagctatattctcggttgcaaggatgggaggcggaccttatctcacctatgtaacactatctgctcacaatcctttcatcattaagggaatggcagtgggactgcagctggtcagtactttctggttctacaagatagcgagaatggtgatgtacaaattgaccaagaggactactagtgcaacgaaggaggctgacatcaaccataaaagagaaaattga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TRAM_LAG1_CLN8

Protein Analysis

237

Amino Acids

26.36

Weight (kDa)

8.96

Isoelectric Point (pI)

39.03

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 319
AciI CCGC 2 cut(s) 112, 525
AclWI GGATC 1 cut(s) 87
AfaI GTAC 2 cut(s) 610, 647
AfiI CCNNNNNNNGG 2 cut(s) 319, 427
AflIII ACRYGT 1 cut(s) 80
AhlI ACTAGT 1 cut(s) 668
AluBI AGCT 3 cut(s) 445, 497, 601
AluI AGCT 3 cut(s) 445, 497, 601
Alw26I GTCTC 1 cut(s) 409
AlwI GGATC 1 cut(s) 87
AoxI GGCC 1 cut(s) 346
ApeKI GCWGC 5 cut(s) 200, 383, 476, 494, 598
AspS9I GGNCC 1 cut(s) 527
AsuHPI GGTGA 2 cut(s) 529, 652
AvaII GGWCC 1 cut(s) 527
BbsI GAAGAC 1 cut(s) 177
BbvCI CCTCAGC 1 cut(s) 216
BbvI GCAGC 5 cut(s) 187, 395, 488, 506, 610
BccI CCATC 2 cut(s) 370, 511
BcoDI GTCTC 1 cut(s) 409
BcuI ACTAGT 1 cut(s) 668
BfaI CTAG 3 cut(s) 233, 408, 669
BfmI CTRYAG 1 cut(s) 596
BisI GCNGC 5 cut(s) 201, 384, 477, 495, 599
BlsI GCNGC 5 cut(s) 202, 385, 478, 496, 600
BmcAI AGTACT 1 cut(s) 610
Bme18I GGWCC 1 cut(s) 527
BmgT120I GGNCC 1 cut(s) 527
BmrI ACTGGG 1 cut(s) 179
BmsI GCATC 1 cut(s) 170
BmuI ACTGGG 1 cut(s) 179
BpiI GAAGAC 1 cut(s) 177
BpmI CTGGAG 1 cut(s) 196
Bpu10I CCTNAGC 1 cut(s) 216
BpuEI CTTGAG 1 cut(s) 445
BsaI GGTCTC 1 cut(s) 409
Bsc4I CCNNNNNNNGG 2 cut(s) 319, 427
Bse1I ACTGG 2 cut(s) 179, 185
Bse3DI GCAATG 2 cut(s) 138, 150
BseGI GGATG 1 cut(s) 522
BseLI CCNNNNNNNGG 2 cut(s) 319, 427
BseMI GCAATG 2 cut(s) 138, 150
BseMII CTCAG 1 cut(s) 230
BseNI ACTGG 2 cut(s) 179, 185
BseRI GAGGAG 1 cut(s) 204
BseXI GCAGC 5 cut(s) 187, 395, 488, 506, 610
BshFI GGCC 1 cut(s) 348
BslFI GGGAC 1 cut(s) 606
BslI CCNNNNNNNGG 2 cut(s) 319, 427
BsmAI GTCTC 1 cut(s) 409
BsmFI GGGAC 1 cut(s) 606
BsnI GGCC 1 cut(s) 348
Bso31I GGTCTC 1 cut(s) 409
Bsp1407I TGTACA 1 cut(s) 645
Bsp143I GATC 3 cut(s) 92, 262, 463
BspACI CCGC 2 cut(s) 112, 525
BspANI GGCC 1 cut(s) 348
BspCNI CTCAG 1 cut(s) 229
BspMAI CTGCAG 1 cut(s) 600
BspPI GGATC 1 cut(s) 87
BspTNI GGTCTC 1 cut(s) 409
BsrDI GCAATG 2 cut(s) 138, 150
BsrGI TGTACA 1 cut(s) 645
BsrI ACTGG 2 cut(s) 179, 185
BssMI GATC 3 cut(s) 92, 262, 463
BstAPI GCANNNNNTGC 2 cut(s) 140, 350
BstAUI TGTACA 1 cut(s) 645
BstC8I GCNNGC 1 cut(s) 346
BstDEI CTNAG 2 cut(s) 216, 472
BstF5I GGATG 1 cut(s) 522
BstKTI GATC 3 cut(s) 95, 265, 466
BstMAI GTCTC 1 cut(s) 409
BstMBI GATC 3 cut(s) 92, 262, 463
BstMWI GCNNNNNNNGC 2 cut(s) 140, 350
BstSFI CTRYAG 1 cut(s) 596
BstV1I GCAGC 5 cut(s) 187, 395, 488, 506, 610
BstV2I GAAGAC 1 cut(s) 177
BstX2I RGATCY 1 cut(s) 92
BstXI CCANNNNNNTGG 1 cut(s) 365
BstYI RGATCY 1 cut(s) 92
BsuRI GGCC 1 cut(s) 348
BtsCI GGATG 1 cut(s) 522
BtsI GCAGTG 2 cut(s) 243, 594
BtsIMutI CAGTG 2 cut(s) 243, 594
Cac8I GCNNGC 1 cut(s) 346
Cfr13I GGNCC 1 cut(s) 527
Csp6I GTAC 2 cut(s) 609, 646
CviAII CATG 3 cut(s) 11, 48, 131
CviJI RGCY 8 cut(s) 200, 243, 348, 411, 445, 497, 601, 686
CviKI_1 RGCY 8 cut(s) 200, 243, 348, 411, 445, 497, 601, 686
CviQI GTAC 2 cut(s) 609, 646
DdeI CTNAG 2 cut(s) 216, 472
DpnI GATC 3 cut(s) 94, 264, 465
DpnII GATC 3 cut(s) 92, 262, 463
EciI GGCGGA 1 cut(s) 540
Eco147I AGGCCT 1 cut(s) 348
Eco31I GGTCTC 1 cut(s) 409
Eco47I GGWCC 1 cut(s) 527
FaeI CATG 3 cut(s) 14, 51, 134
FaiI YATR 9 cut(s) 12, 49, 132, 146, 261, 355, 500, 543, 699
FalI AAGNNNNNCTT 2 cut(s) 80, 112
FaqI GGGAC 1 cut(s) 606
FatI CATG 3 cut(s) 10, 47, 130
Fnu4HI GCNGC 5 cut(s) 201, 384, 477, 495, 599
FokI GGATG 1 cut(s) 529
Fsp4HI GCNGC 5 cut(s) 201, 384, 477, 495, 599
FspBI CTAG 3 cut(s) 233, 408, 669
GluI GCNGC 5 cut(s) 201, 384, 477, 495, 599
GsuI CTGGAG 1 cut(s) 196
HaeIII GGCC 1 cut(s) 348
Hin1II CATG 3 cut(s) 14, 51, 134
HinfI GANTC 2 cut(s) 102, 433
HphI GGTGA 2 cut(s) 529, 652
Hpy188I TCNGA 2 cut(s) 74, 373
Hpy188III TCNNGA 1 cut(s) 299
HpyAV CCTTC 2 cut(s) 424, 673
HpyCH4IV ACGT 1 cut(s) 80
HpyCH4V TGCA 7 cut(s) 108, 134, 353, 494, 512, 598, 674
HpyF10VI GCNNNNNNNGC 2 cut(s) 140, 350
HpyF3I CTNAG 2 cut(s) 216, 472
HpySE526I ACGT 1 cut(s) 80
Hsp92II CATG 3 cut(s) 14, 51, 134
Kzo9I GATC 3 cut(s) 92, 262, 463
LpnPI CCDG 6 cut(s) 160, 182, 198, 330, 587, 601
Lsp1109I GCAGC 5 cut(s) 187, 395, 488, 506, 610
LweI GCATC 1 cut(s) 170
MaeI CTAG 3 cut(s) 233, 408, 669
MaeII ACGT 1 cut(s) 80
MaeIII GTNAC 2 cut(s) 449, 544
MalI GATC 3 cut(s) 94, 264, 465
MboI GATC 3 cut(s) 92, 262, 463
MboII GAAGA 3 cut(s) 182, 201, 301
MflI RGATCY 1 cut(s) 92
MluCI AATT 6 cut(s) 15, 290, 304, 336, 650, 709
MnlI CCTC 8 cut(s) 204, 225, 394, 420, 428, 515, 654, 676
MseI TTAA 1 cut(s) 576
MspA1I CMGCKG 1 cut(s) 601
MwoI GCNNNNNNNGC 2 cut(s) 140, 350
NdeII GATC 3 cut(s) 92, 262, 463
NlaIII CATG 3 cut(s) 14, 51, 134
PceI AGGCCT 1 cut(s) 348
PfeI GAWTC 2 cut(s) 102, 433
PflMI CCANNNNNTGG 1 cut(s) 319
PkrI GCNGC 5 cut(s) 202, 385, 478, 496, 600
PspPI GGNCC 1 cut(s) 527
PstI CTGCAG 1 cut(s) 600
PsuI RGATCY 1 cut(s) 92
PvuII CAGCTG 1 cut(s) 601
RsaI GTAC 2 cut(s) 610, 647
RsaNI GTAC 2 cut(s) 609, 646
SaqAI TTAA 1 cut(s) 576
SatI GCNGC 5 cut(s) 201, 384, 477, 495, 599
Sau3AI GATC 3 cut(s) 92, 262, 463
Sau96I GGNCC 1 cut(s) 527
ScaI AGTACT 1 cut(s) 610
SetI ASST 8 cut(s) 83, 365, 405, 447, 499, 532, 542, 603
SfaNI GCATC 1 cut(s) 170
SfcI CTRYAG 1 cut(s) 596
SinI GGWCC 1 cut(s) 527
SmlI CTYRAG 1 cut(s) 424
SmoI CTYRAG 1 cut(s) 424
SpeI ACTAGT 1 cut(s) 668
Sse9I AATT 6 cut(s) 15, 290, 304, 336, 650, 709
SseBI AGGCCT 1 cut(s) 348
SsiI CCGC 2 cut(s) 112, 525
SspMI CTAG 3 cut(s) 233, 408, 669
StuI AGGCCT 1 cut(s) 348
TaiI ACGT 1 cut(s) 83
TaqI TCGA 2 cut(s) 122, 168
TasI AATT 6 cut(s) 15, 290, 304, 336, 650, 709
TatI WGTACW 2 cut(s) 608, 645
TfiI GAWTC 2 cut(s) 102, 433
Tru1I TTAA 1 cut(s) 576
Tru9I TTAA 1 cut(s) 576
TscAI CASTG 2 cut(s) 243, 594
TseI GCWGC 5 cut(s) 200, 383, 476, 494, 598
TspDTI ATGAA 5 cut(s) 36, 58, 239, 477, 559
TspRI CASTG 2 cut(s) 243, 594
Van91I CCANNNNNTGG 1 cut(s) 319
VpaK11BI GGWCC 1 cut(s) 527
XspI CTAG 3 cut(s) 233, 408, 669
ZrmI AGTACT 1 cut(s) 610
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.