RLG00000011231

Transmembrane protein 136

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0007728
Physical Location & Seq
Reverse (-)
7820590 .. 7821828
1239 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000011231

Sequence Viewer

Length: 636 bp
ATGTATCCAAAGAGATCCTTTGGATTCTGCAACCGCCTTGTTTCGACAATCCATGCAACCATTGCTGTAACCCTAGCTTCACTCTCTGTCGAAGACTGGAGATGCCCAGTTTGTCCTCTGGCTGCTCAATCTTCTCCTCAGCAGATGAAAGCACTCGCAGTGAGCCTTTCTTATCTCATCTATGATCTCATCTGTTGCCTTTTTGACAAGCAATTCAGTCTTGACAATTTCTTTCACCATTTGGTAAGCATTATTGGAATTGTAGCAGGCCTTGCATACCAAAGGTGTGGGTCAGAGATGGTAGCTGCATTGTGGATAACAGAGGTCTCTAGCCCTTTCCTCCACTTGAGGGAGATTCTCAAAGAACTTGGTTACAAGGACACCGACCTAAACTTAGCAGCAGATATTTCATTTGCAGCTATATTCTCGGTTGCAAGGATGGGAGGCGGACCTTATCTCGCTTATGTAACACTATCTGCTCACAATCCTTTCATCATTAAGGGAATGGCAGTTGGACTGCAGCTGGTCAGTACTTTCTGGTTCTACAAGATAGCGAGAATGGTGATGTACAAATTGACCAAGAGGACTACTAGTGCAACAAAGGAGGCTGACATCAACCATAAAAGAGAAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TRAM_LAG1_CLN8

Protein Analysis

212

Amino Acids

23.56

Weight (kDa)

8.88

Isoelectric Point (pI)

44.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TRAM_LAG1_CLN8 PF03798 9 - 186 1.1e-24 TLC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 34, 447
AclWI GGATC 1 cut(s) 9
AfaI GTAC 2 cut(s) 532, 569
AfiI CCNNNNNNNGG 1 cut(s) 349
AhlI ACTAGT 1 cut(s) 590
AluBI AGCT 4 cut(s) 77, 305, 419, 523
AluI AGCT 4 cut(s) 77, 305, 419, 523
Alw26I GTCTC 1 cut(s) 331
AlwI GGATC 1 cut(s) 9
AoxI GGCC 1 cut(s) 268
ApeKI GCWGC 5 cut(s) 122, 305, 398, 416, 520
AspS9I GGNCC 1 cut(s) 449
AsuHPI GGTGA 2 cut(s) 227, 574
AvaII GGWCC 1 cut(s) 449
BbsI GAAGAC 1 cut(s) 99
BbvCI CCTCAGC 1 cut(s) 138
BbvI GCAGC 5 cut(s) 109, 292, 410, 428, 532
BccI CCATC 2 cut(s) 292, 433
BciVI GTATCC 1 cut(s) 15
BcoDI GTCTC 1 cut(s) 331
BcuI ACTAGT 1 cut(s) 590
BfaI CTAG 3 cut(s) 74, 330, 591
BfmI CTRYAG 1 cut(s) 518
BfuI GTATCC 1 cut(s) 15
BisI GCNGC 5 cut(s) 123, 306, 399, 417, 521
BlsI GCNGC 5 cut(s) 124, 307, 400, 418, 522
BmcAI AGTACT 1 cut(s) 532
Bme18I GGWCC 1 cut(s) 449
BmgT120I GGNCC 1 cut(s) 449
BmrI ACTGGG 1 cut(s) 101
BmsI GCATC 1 cut(s) 92
BmuI ACTGGG 1 cut(s) 101
BpiI GAAGAC 1 cut(s) 99
BpmI CTGGAG 1 cut(s) 118
Bpu10I CCTNAGC 1 cut(s) 138
BpuEI CTTGAG 1 cut(s) 367
BsaI GGTCTC 1 cut(s) 331
Bsc4I CCNNNNNNNGG 1 cut(s) 349
Bse1I ACTGG 2 cut(s) 101, 107
Bse3DI GCAATG 1 cut(s) 60
BseGI GGATG 1 cut(s) 444
BseLI CCNNNNNNNGG 1 cut(s) 349
BseMI GCAATG 1 cut(s) 60
BseMII CTCAG 1 cut(s) 152
BseNI ACTGG 2 cut(s) 101, 107
BseRI GAGGAG 1 cut(s) 126
BseXI GCAGC 5 cut(s) 109, 292, 410, 428, 532
BshFI GGCC 1 cut(s) 270
BslI CCNNNNNNNGG 1 cut(s) 349
BsmAI GTCTC 1 cut(s) 331
BsnI GGCC 1 cut(s) 270
Bso31I GGTCTC 1 cut(s) 331
Bsp1407I TGTACA 1 cut(s) 567
Bsp143I GATC 2 cut(s) 14, 184
BspACI CCGC 2 cut(s) 34, 447
BspANI GGCC 1 cut(s) 270
BspCNI CTCAG 1 cut(s) 151
BspMAI CTGCAG 1 cut(s) 522
BspPI GGATC 1 cut(s) 9
BspTNI GGTCTC 1 cut(s) 331
BsrDI GCAATG 1 cut(s) 60
BsrGI TGTACA 1 cut(s) 567
BsrI ACTGG 2 cut(s) 101, 107
BssMI GATC 2 cut(s) 14, 184
BstAPI GCANNNNNTGC 2 cut(s) 62, 272
BstAUI TGTACA 1 cut(s) 567
BstC8I GCNNGC 1 cut(s) 268
BstDEI CTNAG 2 cut(s) 138, 394
BstF5I GGATG 1 cut(s) 444
BstKTI GATC 2 cut(s) 17, 187
BstMAI GTCTC 1 cut(s) 331
BstMBI GATC 2 cut(s) 14, 184
BstMWI GCNNNNNNNGC 2 cut(s) 62, 272
BstSFI CTRYAG 1 cut(s) 518
BstV1I GCAGC 5 cut(s) 109, 292, 410, 428, 532
BstV2I GAAGAC 1 cut(s) 99
BstX2I RGATCY 1 cut(s) 14
BstXI CCANNNNNNTGG 1 cut(s) 287
BstYI RGATCY 1 cut(s) 14
BsuI GTATCC 1 cut(s) 15
BsuRI GGCC 1 cut(s) 270
BtsCI GGATG 1 cut(s) 444
BtsI GCAGTG 1 cut(s) 165
BtsIMutI CAGTG 1 cut(s) 165
Cac8I GCNNGC 1 cut(s) 268
Cfr13I GGNCC 1 cut(s) 449
Csp6I GTAC 2 cut(s) 531, 568
CviAII CATG 1 cut(s) 53
CviJI RGCY 9 cut(s) 77, 122, 165, 270, 305, 333, 419, 523, 608
CviKI_1 RGCY 9 cut(s) 77, 122, 165, 270, 305, 333, 419, 523, 608
CviQI GTAC 2 cut(s) 531, 568
DdeI CTNAG 2 cut(s) 138, 394
DpnI GATC 2 cut(s) 16, 186
DpnII GATC 2 cut(s) 14, 184
EciI GGCGGA 1 cut(s) 462
Eco147I AGGCCT 1 cut(s) 270
Eco31I GGTCTC 1 cut(s) 331
Eco47I GGWCC 1 cut(s) 449
FaeI CATG 1 cut(s) 56
FaiI YATR 6 cut(s) 54, 183, 277, 422, 465, 621
FalI AAGNNNNNCTT 1 cut(s) 34
FatI CATG 1 cut(s) 52
Fnu4HI GCNGC 5 cut(s) 123, 306, 399, 417, 521
FokI GGATG 1 cut(s) 451
Fsp4HI GCNGC 5 cut(s) 123, 306, 399, 417, 521
FspBI CTAG 3 cut(s) 74, 330, 591
GluI GCNGC 5 cut(s) 123, 306, 399, 417, 521
GsuI CTGGAG 1 cut(s) 118
HaeIII GGCC 1 cut(s) 270
Hin1II CATG 1 cut(s) 56
HinfI GANTC 2 cut(s) 24, 355
HphI GGTGA 2 cut(s) 227, 574
Hpy188I TCNGA 1 cut(s) 295
Hpy188III TCNNGA 1 cut(s) 221
HpyCH4V TGCA 8 cut(s) 30, 56, 275, 308, 416, 434, 520, 596
HpyF10VI GCNNNNNNNGC 2 cut(s) 62, 272
HpyF3I CTNAG 2 cut(s) 138, 394
Hsp92II CATG 1 cut(s) 56
Kzo9I GATC 2 cut(s) 14, 184
LpnPI CCDG 6 cut(s) 82, 104, 120, 252, 509, 523
Lsp1109I GCAGC 5 cut(s) 109, 292, 410, 428, 532
LweI GCATC 1 cut(s) 92
MaeI CTAG 3 cut(s) 74, 330, 591
MaeIII GTNAC 3 cut(s) 67, 371, 466
MalI GATC 2 cut(s) 16, 186
MboI GATC 2 cut(s) 14, 184
MboII GAAGA 2 cut(s) 104, 123
MflI RGATCY 1 cut(s) 14
MluCI AATT 5 cut(s) 212, 226, 258, 572, 631
MmeI TCCRAC 1 cut(s) 493
MnlI CCTC 8 cut(s) 126, 147, 316, 342, 350, 437, 576, 598
MseI TTAA 1 cut(s) 498
MspA1I CMGCKG 1 cut(s) 523
MwoI GCNNNNNNNGC 2 cut(s) 62, 272
NdeII GATC 2 cut(s) 14, 184
NlaIII CATG 1 cut(s) 56
PceI AGGCCT 1 cut(s) 270
PfeI GAWTC 2 cut(s) 24, 355
PkrI GCNGC 5 cut(s) 124, 307, 400, 418, 522
PspPI GGNCC 1 cut(s) 449
PstI CTGCAG 1 cut(s) 522
PsuI RGATCY 1 cut(s) 14
PvuII CAGCTG 1 cut(s) 523
RsaI GTAC 2 cut(s) 532, 569
RsaNI GTAC 2 cut(s) 531, 568
SaqAI TTAA 1 cut(s) 498
SatI GCNGC 5 cut(s) 123, 306, 399, 417, 521
Sau3AI GATC 2 cut(s) 14, 184
Sau96I GGNCC 1 cut(s) 449
ScaI AGTACT 1 cut(s) 532
SetI ASST 8 cut(s) 79, 287, 307, 327, 390, 421, 454, 525
SfaNI GCATC 1 cut(s) 92
SfcI CTRYAG 1 cut(s) 518
SinI GGWCC 1 cut(s) 449
SmlI CTYRAG 1 cut(s) 346
SmoI CTYRAG 1 cut(s) 346
SpeI ACTAGT 1 cut(s) 590
Sse9I AATT 5 cut(s) 212, 226, 258, 572, 631
SseBI AGGCCT 1 cut(s) 270
SsiI CCGC 2 cut(s) 34, 447
SspMI CTAG 3 cut(s) 74, 330, 591
StuI AGGCCT 1 cut(s) 270
TaqI TCGA 2 cut(s) 44, 90
TasI AATT 5 cut(s) 212, 226, 258, 572, 631
TatI WGTACW 2 cut(s) 530, 567
TfiI GAWTC 2 cut(s) 24, 355
Tru1I TTAA 1 cut(s) 498
Tru9I TTAA 1 cut(s) 498
TscAI CASTG 1 cut(s) 165
TseI GCWGC 5 cut(s) 122, 305, 398, 416, 520
TspDTI ATGAA 3 cut(s) 161, 399, 481
TspRI CASTG 1 cut(s) 165
VpaK11BI GGWCC 1 cut(s) 449
XspI CTAG 3 cut(s) 74, 330, 591
ZrmI AGTACT 1 cut(s) 532
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.