FvH4_3g04960

Phospholipase A1-Igamma1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0009590
Physical Location & Seq
Reverse (-)
2852867 .. 2854535
1669 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g04960.t1

Sequence Viewer

Length: 1569 bp
ATGGCAATGTTAGCAATGATGCCTGCTCATATTCCGGCGACTCAAGCCGCCTCCGGAGCAACTCGGTTTTCCACGATCCGATCACAGCAGCAGCAAGAAGCTGTTCTAGATCATCACCCGAATACAAAGACATCAAACAGGGCCAATAGTCTGGCCGAGTCACTATCCCACCTCCTTCATCTTCACATAGACACCCCTCCTAAAACCAAGAGTCCTCATCACTTGTCCAATTGGAATATGTTTACTGAAGAGGAGCATTCAACTCCCACAACTTCTCCCAAGGAAGTCATGTCCGAAAAGTGGCGCGAAATCCACGGCTCGAAAGATTGGGAGGGTCTAATGGATCCACTCCACCCTTGGCTAAGAAGAGAGATAGTCAAGTATGGTGAATTTGCACAGGCAACATATGATGCTTTCGACTTTGACTCATTCTCTGAGTTCTGTGGGAGCTGCAGGTACAACAAGAACAAGCTCTTTGATGTGTTGGGATTGAGCAAAAATGGATATGCAGTTAGCAAATACATTTATGCTATGTCGCATATTGACATGCCTAATTGGCTGAAGAGGTCACATTTGGTTGGGACTTGGAGCAAAGATTCAAACTGGATGGGATTTGTTGCAGTGAGTGATGACGAGGAGACTAGAAGGATCGGAAGGAGAGACATTGCGGTGGCTTGGCGTGGGACGGTGGCGCCATCCGAGTGGTATGAGGATATGCAGAGGAAGTTGGAGCCTATTGGGGATTTGGATGCCAAAGTTGAACATGGGTTTCTTGGGATTTACAAGTCCAAGAGTGATGAAACCAGGTACAACAAATCCAGTGCCTCAGAGCAAGTCATGAAGGAAGTGACTAGGCTTGTGGAGTTTTATAAATCCAAGGGTGAAGAAGTGAGTCTTACAATTACAGGGCATAGCTTGGGTGGTGCTTTGGCTTTGCTCAATGCTTATGAAGCTGCAAAAGCAATTCCTGGGCTTCACATTAGTGTCATTTCATTTGGGGCACCCAGAGTGGGAAACAATGCTTTCAGAGATGAGCTAAACCAAATGGGGGTCAAGACACTGAGGGTTGTAGTCAAGCAAGACGTGGTTCCGAAAATGCCCGGACTTGTTTTGAATGAAGGTCTGCAAAAGTTTGAAGAGATTACAGGCACATTGGAATGGGTTTATACACATGTTGGAGCTGAACTGAGAGTTGATGTCAGCTCGTCGCCTTATCTCAAGAAACATGGATTCAATTTTCTAGGGTTTCATAGCCTTGAGACTTATCTTCACCTTGTGGATGGGTATTTGAATAAAGAAACTACCTTTAGGTCAAATGCCAGGAGGGACATTGCCTTGGTGAACAAGGCATGTGACATGTTAGTGGAGGATCTTAGAATCCCACACTGTTGGTACCAAATGGAACACAAGGGACTTGTTTGTAATCATCATGGGAGATGGGTTAAACCCAGAAGAGAGCCTGAAGATATTCCCTCACCTACTAGAGAAGTAGAGGCACAAGCTCATGCTCTTCAAGTAGAGATGCAATCAGATCATGTACTTAAATCTTTATGTACTGCTAATATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

523

Amino Acids

59.28

Weight (kDa)

6.61

Isoelectric Point (pI)

49.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 224 - 369 4.2e-33 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 870
Acc36I ACCTGC 1 cut(s) 444
Acc65I GGTACC 1 cut(s) 1392
AccB1I GGYRCC 3 cut(s) 691, 1000, 1392
AccII CGCG 1 cut(s) 306
AccIII TCCGGA 1 cut(s) 53
AciI CCGC 2 cut(s) 48, 668
AclWI GGATC 5 cut(s) 70, 338, 351, 656, 1377
AcoI YGGCCR 1 cut(s) 153
AcsI RAATTY 1 cut(s) 389
AcuI CTGAAG 3 cut(s) 267, 581, 1482
AcyI GRCGYC 1 cut(s) 692
AdeI CACNNNGTG 1 cut(s) 1276
AfaI GTAC 5 cut(s) 458, 809, 1394, 1539, 1555
AfiI CCNNNNNNNGG 3 cut(s) 300, 1010, 1048
AflIII ACRYGT 2 cut(s) 1171, 1356
AgsI TTSAA 8 cut(s) 261, 600, 761, 1114, 1136, 1234, 1291, 1514
AjiI CACGTC 1 cut(s) 1084
AjnI CCWGG 3 cut(s) 803, 967, 1319
AleI CACNNNNGTG 1 cut(s) 981
AluBI AGCT 9 cut(s) 101, 450, 472, 915, 953, 1036, 1181, 1203, 1502
AluI AGCT 9 cut(s) 101, 450, 472, 915, 953, 1036, 1181, 1203, 1502
Alw26I GTCTC 3 cut(s) 632, 654, 1253
AlwI GGATC 5 cut(s) 70, 338, 351, 656, 1377
Aor13HI TCCGGA 1 cut(s) 53
AoxI GGCC 2 cut(s) 141, 153
ApeKI GCWGC 4 cut(s) 88, 91, 450, 953
ApoI RAATTY 1 cut(s) 389
Asp700I GAANNNNTTC 2 cut(s) 102, 1467
Asp718I GGTACC 1 cut(s) 1392
AspLEI GCGC 2 cut(s) 306, 694
AspS9I GGNCC 1 cut(s) 141
AsuC2I CCSGG 1 cut(s) 1101
AsuHPI GGTGA 6 cut(s) 107, 398, 893, 1262, 1351, 1467
BaeGI GKGCMC 1 cut(s) 1003
BamHI GGATCC 1 cut(s) 343
BanI GGYRCC 3 cut(s) 691, 1000, 1392
BbvI GCAGC 4 cut(s) 100, 103, 437, 940
BccI CCATC 4 cut(s) 601, 703, 1274, 1431
BceAI ACGGC 1 cut(s) 331
BciT130I CCWGG 3 cut(s) 805, 969, 1321
BcnI CCSGG 1 cut(s) 1101
BcoDI GTCTC 3 cut(s) 632, 654, 1253
BfaI CTAG 5 cut(s) 107, 642, 852, 1241, 1482
BfmI CTRYAG 1 cut(s) 451
BfoI RGCGCY 1 cut(s) 695
BfuAI ACCTGC 1 cut(s) 444
BglI GCCNNNNNGGC 1 cut(s) 556
BisI GCNGC 5 cut(s) 48, 89, 92, 451, 954
BlsI GCNGC 5 cut(s) 49, 90, 93, 452, 955
Bme1390I CCNGG 4 cut(s) 805, 969, 1101, 1321
BmgBI CACGTC 1 cut(s) 1084
BmgT120I GGNCC 1 cut(s) 141
BmiI GGNNCC 6 cut(s) 345, 693, 732, 1002, 1089, 1394
BmrFI CCNGG 4 cut(s) 805, 969, 1101, 1321
BmsI GCATC 4 cut(s) 9, 400, 739, 1512
BpuEI CTTGAG 3 cut(s) 27, 1202, 1277
BpuMI CCSGG 1 cut(s) 1101
BsaHI GRCGYC 1 cut(s) 692
BsaJI CCNNGG 6 cut(s) 279, 313, 356, 876, 968, 1335
BsaWI WCCGGW 1 cut(s) 53
BsaXI ACNNNNNCTCC 2 cut(s) 259, 289
Bsc4I CCNNNNNNNGG 3 cut(s) 300, 1010, 1048
Bse1I ACTGG 2 cut(s) 608, 819
Bse3DI GCAATG 4 cut(s) 12, 21, 663, 1329
BseAI TCCGGA 1 cut(s) 53
BseBI CCWGG 3 cut(s) 805, 969, 1321
BseDI CCNNGG 6 cut(s) 279, 313, 356, 876, 968, 1335
BseGI GGATG 4 cut(s) 612, 695, 754, 1285
BseLI CCNNNNNNNGG 3 cut(s) 300, 1010, 1048
BseMI GCAATG 4 cut(s) 12, 21, 663, 1329
BseMII CTCAG 4 cut(s) 426, 840, 1052, 1178
BseNI ACTGG 2 cut(s) 608, 819
BseRI GAGGAG 2 cut(s) 266, 650
BseSI GKGCMC 1 cut(s) 1003
BseXI GCAGC 4 cut(s) 100, 103, 437, 940
Bsh1236I CGCG 1 cut(s) 306
BshFI GGCC 2 cut(s) 143, 155
BshNI GGYRCC 3 cut(s) 691, 1000, 1392
BsiSI CCGG 3 cut(s) 35, 54, 1101
BslFI GGGAC 4 cut(s) 595, 697, 1340, 1425
BslI CCNNNNNNNGG 3 cut(s) 300, 1010, 1048
BsmAI GTCTC 3 cut(s) 632, 654, 1253
BsmFI GGGAC 4 cut(s) 595, 697, 1340, 1425
BsmI GAATGC 1 cut(s) 256
BsnI GGCC 2 cut(s) 143, 155
Bsp1286I GDGCHC 1 cut(s) 1003
Bsp13I TCCGGA 1 cut(s) 53
Bsp143I GATC 7 cut(s) 75, 80, 109, 343, 648, 1369, 1531
BspACI CCGC 2 cut(s) 48, 668
BspANI GGCC 2 cut(s) 143, 155
BspCNI CTCAG 4 cut(s) 427, 839, 1053, 1179
BspEI TCCGGA 1 cut(s) 53
BspFNI CGCG 1 cut(s) 306
BspHI TCATGA 1 cut(s) 837
BspLI GGNNCC 6 cut(s) 345, 693, 732, 1002, 1089, 1394
BspMAI CTGCAG 1 cut(s) 455
BspMI ACCTGC 1 cut(s) 444
BspPI GGATC 5 cut(s) 70, 338, 351, 656, 1377
BspQI GCTCTTC 1 cut(s) 1515
BspT107I GGYRCC 3 cut(s) 691, 1000, 1392
BsrDI GCAATG 4 cut(s) 12, 21, 663, 1329
BsrI ACTGG 2 cut(s) 608, 819
BssECI CCNNGG 6 cut(s) 279, 313, 356, 876, 968, 1335
BssMI GATC 7 cut(s) 75, 80, 109, 343, 648, 1369, 1531
BssNI GRCGYC 1 cut(s) 692
BssT1I CCWWGG 4 cut(s) 279, 356, 876, 1335
Bst2UI CCWGG 3 cut(s) 805, 969, 1321
Bst4CI ACNGT 2 cut(s) 688, 1388
Bst6I CTCTTC 6 cut(s) 243, 361, 557, 1131, 1447, 1515
BstACI GRCGYC 1 cut(s) 692
BstC8I GCNNGC 1 cut(s) 24
BstDEI CTNAG 6 cut(s) 362, 435, 826, 1061, 1187, 1373
BstDSI CCRYGG 1 cut(s) 313
BstF5I GGATG 4 cut(s) 612, 695, 754, 1285
BstFNI CGCG 1 cut(s) 306
BstH2I RGCGCY 1 cut(s) 695
BstHHI GCGC 2 cut(s) 306, 694
BstKTI GATC 7 cut(s) 78, 83, 112, 346, 651, 1372, 1534
BstMAI GTCTC 3 cut(s) 632, 654, 1253
BstMBI GATC 7 cut(s) 75, 80, 109, 343, 648, 1369, 1531
BstMWI GCNNNNNNNGC 6 cut(s) 11, 44, 56, 556, 950, 959
BstNI CCWGG 3 cut(s) 805, 969, 1321
BstNSI RCATGY 4 cut(s) 550, 1175, 1353, 1360
BstSCI CCNGG 4 cut(s) 803, 967, 1099, 1319
BstSFI CTRYAG 1 cut(s) 451
BstSLI GKGCMC 1 cut(s) 1003
BstUI CGCG 1 cut(s) 306
BstV1I GCAGC 4 cut(s) 100, 103, 437, 940
BstX2I RGATCY 2 cut(s) 343, 1369
BstXI CCANNNNNNTGG 3 cut(s) 151, 702, 1389
BstYI RGATCY 2 cut(s) 343, 1369
BsuRI GGCC 2 cut(s) 143, 155
BtgI CCRYGG 1 cut(s) 313
BtrI CACGTC 1 cut(s) 1084
BtsCI GGATG 4 cut(s) 612, 695, 754, 1285
BtsI GCAGTG 1 cut(s) 627
BtsIMutI CAGTG 4 cut(s) 627, 826, 1058, 1384
BveI ACCTGC 1 cut(s) 444
Cac8I GCNNGC 1 cut(s) 24
CciI TCATGA 1 cut(s) 837
CfoI GCGC 2 cut(s) 306, 694
Cfr13I GGNCC 1 cut(s) 141
CsiI ACCWGGT 1 cut(s) 803
Csp6I GTAC 5 cut(s) 457, 808, 1393, 1538, 1554
CviQI GTAC 5 cut(s) 457, 808, 1393, 1538, 1554
DdeI CTNAG 6 cut(s) 362, 435, 826, 1061, 1187, 1373
DinI GGCGCC 1 cut(s) 693
DpnI GATC 7 cut(s) 77, 82, 111, 345, 650, 1371, 1533
DpnII GATC 7 cut(s) 75, 80, 109, 343, 648, 1369, 1531
DraIII CACNNNGTG 1 cut(s) 1276
EaeI YGGCCR 1 cut(s) 153
Eam1104I CTCTTC 6 cut(s) 243, 361, 557, 1131, 1447, 1515
EarI CTCTTC 6 cut(s) 243, 361, 557, 1131, 1447, 1515
Eco130I CCWWGG 4 cut(s) 279, 356, 876, 1335
Eco57I CTGAAG 3 cut(s) 267, 581, 1482
EcoRII CCWGG 3 cut(s) 803, 967, 1319
EcoT14I CCWWGG 4 cut(s) 279, 356, 876, 1335
EgeI GGCGCC 1 cut(s) 693
EheI GGCGCC 1 cut(s) 693
ErhI CCWWGG 4 cut(s) 279, 356, 876, 1335
FalI AAGNNNNNCTT 2 cut(s) 879, 911
FaqI GGGAC 4 cut(s) 595, 697, 1340, 1425
FauNDI CATATG 1 cut(s) 406
Fnu4HI GCNGC 5 cut(s) 48, 89, 92, 451, 954
FokI GGATG 4 cut(s) 619, 682, 761, 1292
Fsp4HI GCNGC 5 cut(s) 48, 89, 92, 451, 954
FspBI CTAG 5 cut(s) 107, 642, 852, 1241, 1482
GlaI GCGC 2 cut(s) 305, 693
GluI GCNGC 5 cut(s) 48, 89, 92, 451, 954
HaeII RGCGCY 1 cut(s) 695
HaeIII GGCC 2 cut(s) 143, 155
HapII CCGG 3 cut(s) 35, 54, 1101
HhaI GCGC 2 cut(s) 306, 694
Hin1I GRCGYC 1 cut(s) 692
Hin6I GCGC 2 cut(s) 304, 692
HinP1I GCGC 2 cut(s) 304, 692
HinfI GANTC 8 cut(s) 40, 158, 211, 425, 596, 892, 1230, 1377
HpaII CCGG 3 cut(s) 35, 54, 1101
HphI GGTGA 6 cut(s) 107, 398, 893, 1262, 1351, 1467
Hpy166II GTNNAC 2 cut(s) 243, 1342
Hpy188I TCNGA 9 cut(s) 80, 295, 436, 653, 700, 829, 1028, 1092, 1531
Hpy188III TCNNGA 5 cut(s) 54, 107, 838, 1054, 1219
Hpy8I GTNNAC 2 cut(s) 243, 1342
Hpy99I CGWCG 1 cut(s) 1210
HpyAV CCTTC 5 cut(s) 185, 639, 648, 835, 1112
HpyCH4III ACNGT 2 cut(s) 688, 1388
HpyCH4IV ACGT 1 cut(s) 1083
HpyCH4V TGCA 8 cut(s) 395, 453, 509, 620, 718, 956, 1126, 1525
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 44, 56, 556, 950, 959
HpyF3I CTNAG 6 cut(s) 362, 435, 826, 1061, 1187, 1373
HpySE526I ACGT 1 cut(s) 1083
Hsp92I GRCGYC 1 cut(s) 692
HspAI GCGC 2 cut(s) 304, 692
KasI GGCGCC 1 cut(s) 691
Kpn2I TCCGGA 1 cut(s) 53
KpnI GGTACC 1 cut(s) 1396
Kzo9I GATC 7 cut(s) 75, 80, 109, 343, 648, 1369, 1531
LguI GCTCTTC 1 cut(s) 1515
LmnI GCTCC 6 cut(s) 56, 253, 447, 588, 730, 1178
Lsp1109I GCAGC 4 cut(s) 100, 103, 437, 940
LweI GCATC 4 cut(s) 9, 400, 739, 1512
MabI ACCWGGT 1 cut(s) 803
MaeI CTAG 5 cut(s) 107, 642, 852, 1241, 1482
MaeII ACGT 1 cut(s) 1083
MaeIII GTNAC 4 cut(s) 159, 567, 847, 1352
MalI GATC 7 cut(s) 77, 82, 111, 345, 650, 1371, 1533
MboI GATC 7 cut(s) 75, 80, 109, 343, 648, 1369, 1531
MfeI CAATTG 1 cut(s) 229
MflI RGATCY 2 cut(s) 343, 1369
MhlI GDGCHC 1 cut(s) 1003
MluCI AATT 6 cut(s) 229, 389, 553, 900, 963, 1234
Mly113I GGCGCC 1 cut(s) 692
MlyI GAGTC 5 cut(s) 34, 167, 220, 419, 901
MmeI TCCRAC 2 cut(s) 708, 1156
MroI TCCGGA 1 cut(s) 53
MroXI GAANNNNTTC 2 cut(s) 102, 1467
MseI TTAA 2 cut(s) 1443, 1542
MslI CAYNNNNRTG 5 cut(s) 668, 700, 981, 1156, 1361
MspI CCGG 3 cut(s) 35, 54, 1101
MspR9I CCNGG 4 cut(s) 805, 969, 1101, 1321
MunI CAATTG 1 cut(s) 229
Mva1269I GAATGC 1 cut(s) 256
MvaI CCWGG 3 cut(s) 805, 969, 1321
MvnI CGCG 1 cut(s) 306
MwoI GCNNNNNNNGC 6 cut(s) 11, 44, 56, 556, 950, 959
NarI GGCGCC 1 cut(s) 692
NciI CCSGG 1 cut(s) 1101
NdeI CATATG 1 cut(s) 406
NdeII GATC 7 cut(s) 75, 80, 109, 343, 648, 1369, 1531
NlaIV GGNNCC 6 cut(s) 345, 693, 732, 1002, 1089, 1394
NmeAIII GCCGAG 1 cut(s) 181
NmuCI GTSAC 4 cut(s) 159, 567, 847, 1352
NspI RCATGY 4 cut(s) 550, 1175, 1353, 1360
OliI CACNNNNGTG 1 cut(s) 981
PagI TCATGA 1 cut(s) 837
PciI ACATGT 2 cut(s) 1171, 1356
PciSI GCTCTTC 1 cut(s) 1515
PctI GAATGC 1 cut(s) 256
PdmI GAANNNNTTC 2 cut(s) 102, 1467
PfeI GAWTC 3 cut(s) 596, 1230, 1377
PkrI GCNGC 5 cut(s) 49, 90, 93, 452, 955
PleI GAGTC 5 cut(s) 34, 166, 219, 419, 900
PluTI GGCGCC 1 cut(s) 695
PpsI GAGTC 5 cut(s) 34, 166, 219, 419, 900
PscI ACATGT 2 cut(s) 1171, 1356
PsiI TTATAA 1 cut(s) 870
Psp6I CCWGG 3 cut(s) 803, 967, 1319
PspGI CCWGG 3 cut(s) 803, 967, 1319
PspN4I GGNNCC 6 cut(s) 345, 693, 732, 1002, 1089, 1394
PspPI GGNCC 1 cut(s) 141
PstI CTGCAG 1 cut(s) 455
PsuI RGATCY 2 cut(s) 343, 1369
RsaI GTAC 5 cut(s) 458, 809, 1394, 1539, 1555
RsaNI GTAC 5 cut(s) 457, 808, 1393, 1538, 1554
RseI CAYNNNNRTG 5 cut(s) 668, 700, 981, 1156, 1361
SapI GCTCTTC 1 cut(s) 1515
SaqAI TTAA 2 cut(s) 1443, 1542
SatI GCNGC 5 cut(s) 48, 89, 92, 451, 954
Sau3AI GATC 7 cut(s) 75, 80, 109, 343, 648, 1369, 1531
Sau96I GGNCC 1 cut(s) 141
SchI GAGTC 5 cut(s) 34, 167, 220, 419, 901
ScrFI CCNGG 4 cut(s) 805, 969, 1101, 1321
SduI GDGCHC 1 cut(s) 1003
SexAI ACCWGGT 1 cut(s) 803
SfaNI GCATC 4 cut(s) 9, 400, 739, 1512
SfcI CTRYAG 1 cut(s) 451
SfoI GGCGCC 1 cut(s) 693
SmiMI CAYNNNNRTG 5 cut(s) 668, 700, 981, 1156, 1361
SmlI CTYRAG 3 cut(s) 42, 1217, 1256
SmoI CTYRAG 3 cut(s) 42, 1217, 1256
Sse9I AATT 6 cut(s) 229, 389, 553, 900, 963, 1234
SsiI CCGC 2 cut(s) 48, 668
SspDI GGCGCC 1 cut(s) 691
SspMI CTAG 5 cut(s) 107, 642, 852, 1241, 1482
StyD4I CCNGG 4 cut(s) 803, 967, 1099, 1319
StyI CCWWGG 4 cut(s) 279, 356, 876, 1335
TaaI ACNGT 2 cut(s) 688, 1388
TaiI ACGT 1 cut(s) 1086
TaqI TCGA 2 cut(s) 320, 417
TasI AATT 6 cut(s) 229, 389, 553, 900, 963, 1234
TatI WGTACW 2 cut(s) 1537, 1553
TauI GCSGC 1 cut(s) 50
TfiI GAWTC 3 cut(s) 596, 1230, 1377
Tru1I TTAA 2 cut(s) 1443, 1542
Tru9I TTAA 2 cut(s) 1443, 1542
TscAI CASTG 4 cut(s) 627, 826, 1065, 1391
TseFI GTSAC 4 cut(s) 159, 567, 847, 1352
TseI GCWGC 4 cut(s) 88, 91, 450, 953
Tsp45I GTSAC 4 cut(s) 159, 567, 847, 1352
TspDTI ATGAA 7 cut(s) 167, 813, 854, 963, 981, 1131, 1238
TspRI CASTG 4 cut(s) 627, 826, 1065, 1391
XapI RAATTY 1 cut(s) 389
XbaI TCTAGA 1 cut(s) 106
XceI RCATGY 4 cut(s) 550, 1175, 1353, 1360
XcmI CCANNNNNNNNNTGG 1 cut(s) 354
XmnI GAANNNNTTC 2 cut(s) 102, 1467
XspI CTAG 5 cut(s) 107, 642, 852, 1241, 1482
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.