MD05G1277700.v1.1

Phospholipase A1-Igamma1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0009590
Physical Location & Seq
Reverse (-)
41235359 .. 41236903
1545 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1277700.v1.1.491

Sequence Viewer

Length: 1545 bp
ATGATATACACATATCTTCCGGCCACTCAAGCCGCCCCCGGTGCAACCCGGTTTTCCCTAATCCGAGCTTGTCATCATCAAGAAGCTGTTCTTGACCATCCCCATCAAACCAAGACATCAAACAAAGCCAACCGTCTAGCTGAATCCCTAGCCAACCTCCTTCACCTCCACATAGATACTCCTCAAAGAACCAACATTAACCCTAATAATTGGAATCTGTTCACGGAAGAGATGCATTCAACTCCCACAACTTCTCCGAAAGACATCATATCCGAAAAATGGCGCGAAATCCACGGGTCCAATGATTGGGAAGGTCTTTTGGACCCTCTCCACCCTTGGCTAAGAAGGGAGATTGTCAAATATGGTGAGTTTGCACAAGCAACGTATGACGCTTTCGATTTTGATTCCTTCTCCGAGTACTGCGGGAGTTGCAGGTACAACAAAAACAAGCTTTTTGATGTGTTGGGTCTAGACAAAAATGGCTACAATGTTAGCAAGTACATTTATGCAATGTCACACATCGACATGCCACAGTGGTTGGAGAGGTCACACTTGGCTGACACTTGGAGCAAGCATTCCAACTGGATGGGGTATGTTGCCGTAAGCGATGAGGAGGAGACTAGGAGGATTGGGAGGAGGGACATTGTGGTGGCGTGGCGGGGGACCGTGGCGCCATCAGAGTGGTATGAGGATATGCAAAGGAAATTGGAGCCGATTGGGAGTGGAGATGCCAAAGTGGAACATGGGTTCCATGGGATTTACACTGCCAAGAGTGACACCACAAGGTACAACAGATCTAGTGCATCAGAGCAAGTCATGAAAGAAGTGGCTAGGCTCGTGGAGTTGTATCAAGCAAGAGGTGAAGAAGTGAGCCTTACAATCACAGGGCATAGCTTGGGAGGTGCATTGGCTTTGCTCAATGCCTATGAAGCTGCCGAAAAACTCCCTGGCCTTCCGATAAGCGTAATTTCCTTTGGTGCCCCTAGGGTGGGAAACATTGCCTTCAAGGAAGAGCTACACCAAATGGGAGTCAAGACATTGAGGGTTGTGGTTAAACAAGACATGGTTCCGAAAATGCCAGGCCTTGTTTTGAATGAGAGTCTTCAGAAGTTTGATGATATTACGGGAACATTGGATTGGGTTTACACACATGTTGGAGCTGAATTGAAGCTCGAAGTTGGTTCTTCGCCTTATCTCAAGCATGGCAGGTTCAATCTGCCCGGGTTTCATAGCCTCGAGACATACCTCCATCTTGTGGATGGGTTTTTTAGTACAGAAACTACTTTTAGATCAAATGCTAGGAGGGATATTGCCTTGGTGAACAAGGGATGCGACATGTTGGTGGATGATCTTAAAATCCCACAGTGTTGGTATCAATTGCCACACAAGGGGCTAGTAAGAAACGCTCATGGGAGATGGGTTAAACCAAAACGAGACCCCGAAGACATACCTTCACCTACAAGAGAAGCACAAGCTCATGCTCTTCAAGGAGAGATGATGCAACCATCATCAGATTATACACTTAAATCTTTATGTGGTACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

515

Amino Acids

58.31

Weight (kDa)

6.37

Isoelectric Point (pI)

43.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 216 - 362 1.8e-33 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 423, 1197
AccB1I GGYRCC 2 cut(s) 670, 977
AccB7I CCANNNNNTGG 2 cut(s) 306, 1255
AccII CGCG 1 cut(s) 285
AciI CCGC 3 cut(s) 33, 423, 658
AcoI YGGCCR 1 cut(s) 21
AcuI CTGAAG 1 cut(s) 1088
AcyI GRCGYC 1 cut(s) 671
AfaI GTAC 6 cut(s) 419, 437, 500, 788, 1273, 1540
AfiI CCNNNNNNNGG 6 cut(s) 279, 306, 988, 989, 1255, 1388
AflIII ACRYGT 2 cut(s) 1150, 1335
AgsI TTSAA 6 cut(s) 240, 1006, 1093, 1168, 1213, 1487
AjnI CCWGG 2 cut(s) 946, 1078
AjuI GAANNNNNNNTTGG 2 cut(s) 1120, 1152
AloI GAACNNNNNNTCC 4 cut(s) 731, 732, 763, 764
Alw26I GTCTC 3 cut(s) 611, 1232, 1428
Ama87I CYCGRG 2 cut(s) 1220, 1235
AoxI GGCC 3 cut(s) 21, 949, 1081
ApeKI GCWGC 1 cut(s) 932
Asp700I GAANNNNTTC 2 cut(s) 87, 218
AspA2I CCTAGG 1 cut(s) 983
AspLEI GCGC 2 cut(s) 285, 673
AspS9I GGNCC 3 cut(s) 297, 322, 663
AsuC2I CCSGG 4 cut(s) 39, 49, 1221, 1222
AsuHPI GGTGA 5 cut(s) 155, 377, 872, 1330, 1446
AvaI CYCGRG 2 cut(s) 1220, 1235
AvaII GGWCC 3 cut(s) 297, 322, 663
AvrII CCTAGG 1 cut(s) 983
BaeGI GKGCMC 1 cut(s) 982
BanI GGYRCC 2 cut(s) 670, 977
BauI CACGAG 1 cut(s) 836
BbsI GAAGAC 2 cut(s) 1094, 1449
BbvI GCAGC 1 cut(s) 919
BccI CCATC 8 cut(s) 105, 111, 580, 682, 1253, 1257, 1410, 1513
BceAI ACGGC 1 cut(s) 584
BciT130I CCWGG 2 cut(s) 948, 1080
BcnI CCSGG 4 cut(s) 39, 49, 1221, 1222
BcoDI GTCTC 3 cut(s) 611, 1232, 1428
BfaI CTAG 9 cut(s) 137, 149, 470, 621, 798, 831, 984, 1299, 1394
BfoI RGCGCY 1 cut(s) 674
BfuAI ACCTGC 2 cut(s) 423, 1197
BglII AGATCT 1 cut(s) 794
BisI GCNGC 2 cut(s) 33, 933
BlnI CCTAGG 1 cut(s) 983
BlsI GCNGC 2 cut(s) 34, 934
BmcAI AGTACT 1 cut(s) 419
Bme1390I CCNGG 6 cut(s) 39, 49, 948, 1080, 1221, 1222
Bme18I GGWCC 3 cut(s) 297, 322, 663
BmeT110I CYCGRG 2 cut(s) 1220, 1235
BmgT120I GGNCC 3 cut(s) 297, 322, 663
BmiI GGNNCC 8 cut(s) 298, 324, 664, 672, 711, 749, 979, 1068
BmrFI CCNGG 6 cut(s) 39, 49, 948, 1080, 1221, 1222
BmsI GCATC 5 cut(s) 222, 718, 812, 1319, 1488
BpiI GAAGAC 2 cut(s) 1094, 1449
BpuEI CTTGAG 2 cut(s) 12, 1181
BpuMI CCSGG 4 cut(s) 39, 49, 1221, 1222
BsaHI GRCGYC 1 cut(s) 671
BsaI GGTCTC 1 cut(s) 1428
BsaJI CCNNGG 9 cut(s) 37, 292, 335, 666, 751, 946, 983, 1220, 1314
BsaXI ACNNNNNCTCC 2 cut(s) 238, 268
Bsc4I CCNNNNNNNGG 6 cut(s) 279, 306, 988, 989, 1255, 1388
Bse1I ACTGG 1 cut(s) 587
Bse3DI GCAATG 2 cut(s) 516, 996
BseBI CCWGG 2 cut(s) 948, 1080
BseDI CCNNGG 9 cut(s) 37, 292, 335, 666, 751, 946, 983, 1220, 1314
BseGI GGATG 5 cut(s) 97, 591, 1264, 1334, 1351
BseLI CCNNNNNNNGG 6 cut(s) 279, 306, 988, 989, 1255, 1388
BseMI GCAATG 2 cut(s) 516, 996
BseNI ACTGG 1 cut(s) 587
BseRI GAGGAG 4 cut(s) 171, 626, 629, 649
BseSI GKGCMC 1 cut(s) 982
BseXI GCAGC 1 cut(s) 919
Bsh1236I CGCG 1 cut(s) 285
BshFI GGCC 3 cut(s) 23, 951, 1083
BshNI GGYRCC 2 cut(s) 670, 977
BsiHKCI CYCGRG 2 cut(s) 1220, 1235
BsiSI CCGG 4 cut(s) 20, 39, 49, 1221
BslFI GGGAC 2 cut(s) 653, 676
BslI CCNNNNNNNGG 6 cut(s) 279, 306, 988, 989, 1255, 1388
BsmAI GTCTC 3 cut(s) 611, 1232, 1428
BsmFI GGGAC 2 cut(s) 653, 676
BsmI GAATGC 2 cut(s) 235, 574
BsnI GGCC 3 cut(s) 23, 951, 1083
Bso31I GGTCTC 1 cut(s) 1428
BsoBI CYCGRG 2 cut(s) 1220, 1235
Bsp1286I GDGCHC 1 cut(s) 982
Bsp143I GATC 3 cut(s) 794, 1289, 1348
Bsp19I CCATGG 1 cut(s) 751
BspACI CCGC 3 cut(s) 33, 423, 658
BspANI GGCC 3 cut(s) 23, 951, 1083
BspFNI CGCG 1 cut(s) 285
BspHI TCATGA 1 cut(s) 816
BspLI GGNNCC 8 cut(s) 298, 324, 664, 672, 711, 749, 979, 1068
BspMI ACCTGC 2 cut(s) 423, 1197
BspQI GCTCTTC 2 cut(s) 1005, 1488
BspT107I GGYRCC 2 cut(s) 670, 977
BspTNI GGTCTC 1 cut(s) 1428
BsrDI GCAATG 2 cut(s) 516, 996
BsrI ACTGG 1 cut(s) 587
BssECI CCNNGG 9 cut(s) 37, 292, 335, 666, 751, 946, 983, 1220, 1314
BssMI GATC 3 cut(s) 794, 1289, 1348
BssNI GRCGYC 1 cut(s) 671
BssSI CACGAG 1 cut(s) 836
BssT1I CCWWGG 4 cut(s) 335, 751, 983, 1314
Bst2BI CACGAG 1 cut(s) 836
Bst2UI CCWGG 2 cut(s) 948, 1080
Bst4CI ACNGT 4 cut(s) 134, 534, 667, 1365
Bst6I CTCTTC 3 cut(s) 222, 1005, 1488
BstACI GRCGYC 1 cut(s) 671
BstC8I GCNNGC 1 cut(s) 572
BstDEI CTNAG 1 cut(s) 341
BstDSI CCRYGG 3 cut(s) 292, 666, 751
BstF5I GGATG 5 cut(s) 97, 591, 1264, 1334, 1351
BstFNI CGCG 1 cut(s) 285
BstH2I RGCGCY 1 cut(s) 674
BstHHI GCGC 2 cut(s) 285, 673
BstKTI GATC 3 cut(s) 797, 1292, 1351
BstMAI GTCTC 3 cut(s) 611, 1232, 1428
BstMBI GATC 3 cut(s) 794, 1289, 1348
BstMWI GCNNNNNNNGC 4 cut(s) 29, 41, 429, 929
BstNI CCWGG 2 cut(s) 948, 1080
BstNSI RCATGY 3 cut(s) 529, 1154, 1339
BstSCI CCNGG 6 cut(s) 37, 47, 946, 1078, 1219, 1220
BstSLI GKGCMC 1 cut(s) 982
BstUI CGCG 1 cut(s) 285
BstV1I GCAGC 1 cut(s) 919
BstV2I GAAGAC 2 cut(s) 1094, 1449
BstX2I RGATCY 1 cut(s) 794
BstXI CCANNNNNNTGG 3 cut(s) 586, 681, 1368
BstYI RGATCY 1 cut(s) 794
BsuRI GGCC 3 cut(s) 23, 951, 1083
BtgI CCRYGG 3 cut(s) 292, 666, 751
BtgZI GCGATG 1 cut(s) 621
BtsCI GGATG 5 cut(s) 97, 591, 1264, 1334, 1351
BtsI GCAGTG 1 cut(s) 762
BtsIMutI CAGTG 3 cut(s) 539, 762, 1370
BveI ACCTGC 2 cut(s) 423, 1197
Cac8I GCNNGC 1 cut(s) 572
CciI TCATGA 1 cut(s) 816
CfoI GCGC 2 cut(s) 285, 673
Cfr13I GGNCC 3 cut(s) 297, 322, 663
Cfr9I CCCGGG 1 cut(s) 1220
CseI GACGC 1 cut(s) 398
Csp6I GTAC 6 cut(s) 418, 436, 499, 787, 1272, 1539
CspCI CAANNNNNGTGG 2 cut(s) 519, 554
CviQI GTAC 6 cut(s) 418, 436, 499, 787, 1272, 1539
DdeI CTNAG 1 cut(s) 341
DinI GGCGCC 1 cut(s) 672
DpnI GATC 3 cut(s) 796, 1291, 1350
DpnII GATC 3 cut(s) 794, 1289, 1348
EaeI YGGCCR 1 cut(s) 21
Eam1104I CTCTTC 3 cut(s) 222, 1005, 1488
EarI CTCTTC 3 cut(s) 222, 1005, 1488
Eco130I CCWWGG 4 cut(s) 335, 751, 983, 1314
Eco147I AGGCCT 1 cut(s) 1083
Eco31I GGTCTC 1 cut(s) 1428
Eco47I GGWCC 3 cut(s) 297, 322, 663
Eco57I CTGAAG 1 cut(s) 1088
Eco88I CYCGRG 2 cut(s) 1220, 1235
EcoRII CCWGG 2 cut(s) 946, 1078
EcoT14I CCWWGG 4 cut(s) 335, 751, 983, 1314
EcoT22I ATGCAT 1 cut(s) 237
EgeI GGCGCC 1 cut(s) 672
EheI GGCGCC 1 cut(s) 672
ErhI CCWWGG 4 cut(s) 335, 751, 983, 1314
FalI AAGNNNNNCTT 4 cut(s) 75, 107, 858, 890
FaqI GGGAC 2 cut(s) 653, 676
FauI CCCGC 2 cut(s) 416, 651
Fnu4HI GCNGC 2 cut(s) 33, 933
FokI GGATG 5 cut(s) 84, 598, 1271, 1341, 1358
Fsp4HI GCNGC 2 cut(s) 33, 933
FspBI CTAG 9 cut(s) 137, 149, 470, 621, 798, 831, 984, 1299, 1394
GlaI GCGC 2 cut(s) 284, 672
GluI GCNGC 2 cut(s) 33, 933
HaeII RGCGCY 1 cut(s) 674
HaeIII GGCC 3 cut(s) 23, 951, 1083
HapII CCGG 4 cut(s) 20, 39, 49, 1221
HgaI GACGC 1 cut(s) 398
HhaI GCGC 2 cut(s) 285, 673
Hin1I GRCGYC 1 cut(s) 671
Hin6I GCGC 2 cut(s) 283, 671
HinP1I GCGC 2 cut(s) 283, 671
HindIII AAGCTT 1 cut(s) 449
HinfI GANTC 5 cut(s) 143, 214, 404, 1029, 1099
HpaII CCGG 4 cut(s) 20, 39, 49, 1221
HphI GGTGA 5 cut(s) 155, 377, 872, 1330, 1446
Hpy166II GTNNAC 3 cut(s) 222, 1144, 1321
Hpy188III TCNNGA 6 cut(s) 80, 92, 470, 817, 1033, 1237
Hpy8I GTNNAC 3 cut(s) 222, 1144, 1321
HpyAV CCTTC 7 cut(s) 170, 305, 339, 418, 962, 1012, 1461
HpyCH4III ACNGT 4 cut(s) 134, 534, 667, 1365
HpyCH4IV ACGT 1 cut(s) 383
HpyCH4V TGCA 9 cut(s) 44, 235, 374, 432, 509, 697, 803, 905, 1501
HpyF10VI GCNNNNNNNGC 4 cut(s) 29, 41, 429, 929
HpyF3I CTNAG 1 cut(s) 341
HpySE526I ACGT 1 cut(s) 383
Hsp92I GRCGYC 1 cut(s) 671
HspAI GCGC 2 cut(s) 283, 671
KasI GGCGCC 1 cut(s) 670
Kzo9I GATC 3 cut(s) 794, 1289, 1348
LguI GCTCTTC 2 cut(s) 1005, 1488
LmnI GCTCC 3 cut(s) 567, 709, 1157
Lsp1109I GCAGC 1 cut(s) 919
LweI GCATC 5 cut(s) 222, 718, 812, 1319, 1488
MaeI CTAG 9 cut(s) 137, 149, 470, 621, 798, 831, 984, 1299, 1394
MaeII ACGT 1 cut(s) 383
MaeIII GTNAC 3 cut(s) 513, 546, 773
MalI GATC 3 cut(s) 796, 1291, 1350
MboI GATC 3 cut(s) 794, 1289, 1348
MboII GAAGA 8 cut(s) 8, 239, 875, 1022, 1094, 1176, 1454, 1475
MfeI CAATTG 1 cut(s) 1376
MflI RGATCY 1 cut(s) 794
MhlI GDGCHC 1 cut(s) 982
MluCI AATT 5 cut(s) 208, 704, 966, 1163, 1376
Mly113I GGCGCC 1 cut(s) 671
MlyI GAGTC 2 cut(s) 1038, 1108
MmeI TCCRAC 3 cut(s) 519, 603, 1135
Mph1103I ATGCAT 1 cut(s) 237
MroXI GAANNNNTTC 2 cut(s) 87, 218
MseI TTAA 6 cut(s) 198, 1053, 1353, 1422, 1524, 1543
MslI CAYNNNNRTG 4 cut(s) 524, 647, 679, 1340
MspI CCGG 4 cut(s) 20, 39, 49, 1221
MspR9I CCNGG 6 cut(s) 39, 49, 948, 1080, 1221, 1222
MunI CAATTG 1 cut(s) 1376
Mva1269I GAATGC 2 cut(s) 235, 574
MvaI CCWGG 2 cut(s) 948, 1080
MvnI CGCG 1 cut(s) 285
MwoI GCNNNNNNNGC 4 cut(s) 29, 41, 429, 929
NarI GGCGCC 1 cut(s) 671
NciI CCSGG 4 cut(s) 39, 49, 1221, 1222
NcoI CCATGG 1 cut(s) 751
NdeII GATC 3 cut(s) 794, 1289, 1348
NlaIV GGNNCC 8 cut(s) 298, 324, 664, 672, 711, 749, 979, 1068
NmuCI GTSAC 3 cut(s) 513, 546, 773
NsiI ATGCAT 1 cut(s) 237
NspI RCATGY 3 cut(s) 529, 1154, 1339
PaeR7I CTCGAG 1 cut(s) 1235
PagI TCATGA 1 cut(s) 816
PceI AGGCCT 1 cut(s) 1083
PciI ACATGT 2 cut(s) 1150, 1335
PciSI GCTCTTC 2 cut(s) 1005, 1488
PctI GAATGC 2 cut(s) 235, 574
PdmI GAANNNNTTC 2 cut(s) 87, 218
PfeI GAWTC 3 cut(s) 143, 214, 404
PflMI CCANNNNNTGG 2 cut(s) 306, 1255
PkrI GCNGC 2 cut(s) 34, 934
PleI GAGTC 2 cut(s) 1037, 1107
PluTI GGCGCC 1 cut(s) 674
PpsI GAGTC 2 cut(s) 1037, 1107
PscI ACATGT 2 cut(s) 1150, 1335
Psp6I CCWGG 2 cut(s) 946, 1078
PspGI CCWGG 2 cut(s) 946, 1078
PspN4I GGNNCC 8 cut(s) 298, 324, 664, 672, 711, 749, 979, 1068
PspPI GGNCC 3 cut(s) 297, 322, 663
PsuI RGATCY 1 cut(s) 794
RsaI GTAC 6 cut(s) 419, 437, 500, 788, 1273, 1540
RsaNI GTAC 6 cut(s) 418, 436, 499, 787, 1272, 1539
RseI CAYNNNNRTG 4 cut(s) 524, 647, 679, 1340
SapI GCTCTTC 2 cut(s) 1005, 1488
SaqAI TTAA 6 cut(s) 198, 1053, 1353, 1422, 1524, 1543
SatI GCNGC 2 cut(s) 33, 933
Sau3AI GATC 3 cut(s) 794, 1289, 1348
Sau96I GGNCC 3 cut(s) 297, 322, 663
ScaI AGTACT 1 cut(s) 419
SchI GAGTC 2 cut(s) 1038, 1108
ScrFI CCNGG 6 cut(s) 39, 49, 948, 1080, 1221, 1222
SduI GDGCHC 1 cut(s) 982
SfaNI GCATC 5 cut(s) 222, 718, 812, 1319, 1488
SfoI GGCGCC 1 cut(s) 672
Sfr274I CTCGAG 1 cut(s) 1235
SinI GGWCC 3 cut(s) 297, 322, 663
SlaI CTCGAG 1 cut(s) 1235
SmaI CCCGGG 1 cut(s) 1222
SmiMI CAYNNNNRTG 4 cut(s) 524, 647, 679, 1340
SmlI CTYRAG 3 cut(s) 27, 1196, 1235
SmoI CTYRAG 3 cut(s) 27, 1196, 1235
Sse9I AATT 5 cut(s) 208, 704, 966, 1163, 1376
SseBI AGGCCT 1 cut(s) 1083
SsiI CCGC 3 cut(s) 33, 423, 658
SspDI GGCGCC 1 cut(s) 670
SspMI CTAG 9 cut(s) 137, 149, 470, 621, 798, 831, 984, 1299, 1394
StuI AGGCCT 1 cut(s) 1083
StyD4I CCNGG 6 cut(s) 37, 47, 946, 1078, 1219, 1220
StyI CCWWGG 4 cut(s) 335, 751, 983, 1314
TaaI ACNGT 4 cut(s) 134, 534, 667, 1365
TaiI ACGT 1 cut(s) 386
TaqI TCGA 4 cut(s) 396, 522, 1173, 1236
TasI AATT 5 cut(s) 208, 704, 966, 1163, 1376
TatI WGTACW 3 cut(s) 417, 498, 1271
TauI GCSGC 1 cut(s) 35
TfiI GAWTC 3 cut(s) 143, 214, 404
Tru1I TTAA 6 cut(s) 198, 1053, 1353, 1422, 1524, 1543
Tru9I TTAA 6 cut(s) 198, 1053, 1353, 1422, 1524, 1543
TscAI CASTG 3 cut(s) 539, 769, 1370
TseFI GTSAC 3 cut(s) 513, 546, 773
TseI GCWGC 1 cut(s) 932
Tsp45I GTSAC 3 cut(s) 513, 546, 773
TspDTI ATGAA 3 cut(s) 833, 942, 1217
TspGWI ACGGA 1 cut(s) 239
TspMI CCCGGG 1 cut(s) 1220
TspRI CASTG 3 cut(s) 539, 769, 1370
Van91I CCANNNNNTGG 2 cut(s) 306, 1255
VpaK11BI GGWCC 3 cut(s) 297, 322, 663
XbaI TCTAGA 1 cut(s) 469
XceI RCATGY 3 cut(s) 529, 1154, 1339
XcmI CCANNNNNNNNNTGG 1 cut(s) 1256
XhoI CTCGAG 1 cut(s) 1235
XmaI CCCGGG 1 cut(s) 1220
XmaJI CCTAGG 1 cut(s) 983
XmnI GAANNNNTTC 2 cut(s) 87, 218
XspI CTAG 9 cut(s) 137, 149, 470, 621, 798, 831, 984, 1299, 1394
ZrmI AGTACT 1 cut(s) 419
Zsp2I ATGCAT 1 cut(s) 237
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.