pycom05g25880

Phospholipase A1-Igamma1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0009590
Physical Location & Seq
Reverse (-)
27190227 .. 27191792
1566 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g25880.1

Sequence Viewer

Length: 1566 bp
ATGGCGCTATCAACTATGATGCACACCCATCTTCCGCCCGCTCAAGCCACTCCCAGCGCAACCCGGTTTTCCCAAATCCGAGCTAGTCATCATCAGGAAGCTGATTTTGATCATCCCCATCAAACCAAGACATCAAACAAAGCCAACCGTCTAGCTAAGTCGTTTGCCAACCTCCTTCACCTCCACATAGATATTCCTCAAAGAACCAACATTAACCCTAATAATTGGAATCTCCTCATTGAAGAGATGCATTCAACTCCCACAACTTCTCCGAAAGACGTCATGTCCGAAAAATGGCGTGAAATACACGGGTCAAATGATTGGGAGGGTCTTTTGGACCCTCTCCATCCTTGGCTAAGAAGAGAGATTGTCAAATATGGTGAGTTTGCACAAGCAACGTATGACGCTTTCGATTTCGATTCCTTCTCCGAGTACTGCGGGAGCTGCAGGTACAACAAAAACAAGCTCTTTGATGTGTTGCGTCTAGACAAAAATGGCTACAATGTTAGCAAGTACATTTATGCAATGTCGCACATCGACATGCCACAGTGGCTGGAGAGGTCACACTTGGTTGACACATGGAGCAAGCATTCAAACTGGATGGGTTATGTTGCCGTGAGCGATGAGGAGGAGACTAGGAGGATTGGGAGGAGGGACATAGTGGTGGCGTGGCGGGGGACCGTGGTGCCATCGGAGTGGTATGAGGATATGCAAAGGAAATTGGAGCCAATTGGGAGTGGAGATGCCAAAGTGGAACATGGGTTCCATAGGATTTACACTGCCAAGAGTAACACCACAAGGTACAACAGGTCCAGTGCATCAGAGCAAGTCATGAAAGAAGTGACTAGGCTTGTTGAGTTGTGCCAAGCAAAAGGTGAAGAAGTGAGCCTAACAATCACAGGGCATAGCTTGGGAGGTGCATTGGCTTTGCTCAATGCCTATGAAGCTGCCGAAAAATTCCCTGGCCTTACGATAAGCGTAATTTCCTTCGGAGCCCCTAGGGTTGGAAACATGGCCTTCAAGGAAGAGCTACACCAAATGGGAGTCAAGACATTGAGGGTTGTGGTTAAACAAGACATGGTTCCGAAAATGCCGGGGCTTGTTTTGAATGAGAGTCTTCAAAAGTTTGATGACATTACGGGCACATTGGATTGGGTTTACGCACATGTTGGAGCTGAATTGAAGCTCGAAATTGGTTCTTCGCCTTATCTCAAGCATGGCGGGTTCAATCTGCCTGGTTTTCATAGCCTCGAGACCTACCTTCATCTTGTGGATGGGTTTTTGAGTACAGAAACTACTTTTAGGTCAAATGTCAGGAGGGATATTACCTTGGTGAACAAGGGATGTGACATGTTGGTGGATGATCTCAAAATCCCACAATGTTGGTACCAATTGCCACACAAGGGGTTAGTAAGAAACGCTCATGGGAGATGGGTTAAACCGAAACGAGACCCCGAAGACATCCCTTCACCTGGAAGAGAAGCACAAGCACAAGCTCATGCTCTTCAAGTAGAGATGGTGCAATCAACATCAGATTATACACACAAATCTTTATGTAGTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

522

Amino Acids

59.28

Weight (kDa)

6.7

Isoelectric Point (pI)

47.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 221 - 367 8.6e-33 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 282
Acc36I ACCTGC 1 cut(s) 438
Acc65I GGTACC 1 cut(s) 1386
AccB1I GGYRCC 2 cut(s) 685, 1386
AccBSI CCGCTC 1 cut(s) 41
AciI CCGC 5 cut(s) 35, 39, 438, 673, 1221
AcsI RAATTY 1 cut(s) 956
AcyI GRCGYC 1 cut(s) 279
AfaI GTAC 6 cut(s) 434, 452, 515, 803, 1288, 1388
AfiI CCNNNNNNNGG 4 cut(s) 294, 1004, 1403, 1472
AflIII ACRYGT 2 cut(s) 1165, 1350
AgsI TTSAA 9 cut(s) 242, 255, 594, 1021, 1108, 1121, 1183, 1228, 1508
AhdI GACNNNNNGTC 1 cut(s) 283
AjnI CCWGG 3 cut(s) 961, 1234, 1471
AloI GAACNNNNNNTCC 4 cut(s) 746, 747, 778, 779
Alw26I GTCTC 3 cut(s) 626, 1247, 1443
AlwNI CAGNNNCTG 1 cut(s) 553
Ama87I CYCGRG 1 cut(s) 1250
AoxI GGCC 2 cut(s) 964, 1014
ApeKI GCWGC 2 cut(s) 444, 947
ApoI RAATTY 1 cut(s) 956
Asp718I GGTACC 1 cut(s) 1386
AspA2I CCTAGG 1 cut(s) 998
AspLEI GCGC 2 cut(s) 7, 59
AspS9I GGNCC 3 cut(s) 337, 678, 810
AsuC2I CCSGG 2 cut(s) 64, 1095
AsuHPI GGTGA 5 cut(s) 170, 392, 887, 1345, 1461
AvaI CYCGRG 1 cut(s) 1250
AvaII GGWCC 3 cut(s) 337, 678, 810
AvrII CCTAGG 1 cut(s) 998
BaeGI GKGCMC 1 cut(s) 1145
BanI GGYRCC 2 cut(s) 685, 1386
BanII GRGCYC 1 cut(s) 997
BbsI GAAGAC 2 cut(s) 1109, 1464
BbvI GCAGC 2 cut(s) 431, 934
BccI CCATC 8 cut(s) 36, 126, 354, 595, 697, 1268, 1425, 1510
BceAI ACGGC 1 cut(s) 599
BciT130I CCWGG 3 cut(s) 963, 1236, 1473
BclI TGATCA 1 cut(s) 109
BcnI CCSGG 2 cut(s) 64, 1095
BcoDI GTCTC 3 cut(s) 626, 1247, 1443
BfaI CTAG 6 cut(s) 84, 152, 485, 636, 846, 999
BfmI CTRYAG 1 cut(s) 445
BfoI RGCGCY 1 cut(s) 8
BfuAI ACCTGC 1 cut(s) 438
BglI GCCNNNNNGGC 1 cut(s) 550
BisI GCNGC 2 cut(s) 445, 948
BlnI CCTAGG 1 cut(s) 998
BlsI GCNGC 2 cut(s) 446, 949
BmcAI AGTACT 1 cut(s) 434
Bme1390I CCNGG 5 cut(s) 64, 963, 1095, 1236, 1473
Bme18I GGWCC 3 cut(s) 337, 678, 810
BmeRI GACNNNNNGTC 1 cut(s) 283
BmeT110I CYCGRG 1 cut(s) 1250
BmgT120I GGNCC 3 cut(s) 337, 678, 810
BmiI GGNNCC 8 cut(s) 339, 679, 687, 726, 764, 994, 1083, 1388
BmrFI CCNGG 5 cut(s) 64, 963, 1095, 1236, 1473
BmsI GCATC 4 cut(s) 9, 237, 733, 827
BpiI GAAGAC 2 cut(s) 1109, 1464
BpmI CTGGAG 1 cut(s) 575
BpuEI CTTGAG 2 cut(s) 27, 1196
BpuMI CCSGG 2 cut(s) 64, 1095
BsaBI GATNNNNATC 1 cut(s) 108
BsaHI GRCGYC 1 cut(s) 279
BsaI GGTCTC 2 cut(s) 1247, 1443
BsaJI CCNNGG 6 cut(s) 350, 681, 961, 998, 1094, 1329
BsaXI ACNNNNNCTCC 2 cut(s) 253, 283
Bsc4I CCNNNNNNNGG 4 cut(s) 294, 1004, 1403, 1472
Bse1I ACTGG 2 cut(s) 602, 813
Bse3DI GCAATG 1 cut(s) 531
Bse8I GATNNNNATC 1 cut(s) 108
BseBI CCWGG 3 cut(s) 963, 1236, 1473
BseDI CCNNGG 6 cut(s) 350, 681, 961, 998, 1094, 1329
BseGI GGATG 7 cut(s) 112, 346, 606, 1279, 1349, 1366, 1461
BseJI GATNNNNATC 1 cut(s) 108
BseLI CCNNNNNNNGG 4 cut(s) 294, 1004, 1403, 1472
BseMI GCAATG 1 cut(s) 531
BseNI ACTGG 2 cut(s) 602, 813
BseRI GAGGAG 4 cut(s) 224, 641, 644, 664
BseSI GKGCMC 1 cut(s) 1145
BseXI GCAGC 2 cut(s) 431, 934
BseYI CCCAGC 1 cut(s) 53
BshFI GGCC 2 cut(s) 966, 1016
BshNI GGYRCC 2 cut(s) 685, 1386
BsiHKCI CYCGRG 1 cut(s) 1250
BsiSI CCGG 2 cut(s) 64, 1094
BslFI GGGAC 2 cut(s) 668, 691
BslI CCNNNNNNNGG 4 cut(s) 294, 1004, 1403, 1472
BsmAI GTCTC 3 cut(s) 626, 1247, 1443
BsmFI GGGAC 2 cut(s) 668, 691
BsmI GAATGC 2 cut(s) 250, 589
BsnI GGCC 2 cut(s) 966, 1016
Bso31I GGTCTC 2 cut(s) 1247, 1443
BsoBI CYCGRG 1 cut(s) 1250
Bsp1286I GDGCHC 2 cut(s) 997, 1145
Bsp143I GATC 2 cut(s) 109, 1363
BspACI CCGC 5 cut(s) 35, 39, 438, 673, 1221
BspANI GGCC 2 cut(s) 966, 1016
BspHI TCATGA 1 cut(s) 831
BspLI GGNNCC 8 cut(s) 339, 679, 687, 726, 764, 994, 1083, 1388
BspMAI CTGCAG 1 cut(s) 449
BspMI ACCTGC 1 cut(s) 438
BspQI GCTCTTC 2 cut(s) 1020, 1509
BspT107I GGYRCC 2 cut(s) 685, 1386
BspTNI GGTCTC 2 cut(s) 1247, 1443
BsrBI CCGCTC 1 cut(s) 41
BsrDI GCAATG 1 cut(s) 531
BsrI ACTGG 2 cut(s) 602, 813
BssECI CCNNGG 6 cut(s) 350, 681, 961, 998, 1094, 1329
BssMI GATC 2 cut(s) 109, 1363
BssNI GRCGYC 1 cut(s) 279
BssT1I CCWWGG 3 cut(s) 350, 998, 1329
Bst2UI CCWGG 3 cut(s) 963, 1236, 1473
Bst4CI ACNGT 3 cut(s) 149, 549, 682
Bst6I CTCTTC 5 cut(s) 237, 355, 1020, 1471, 1509
BstACI GRCGYC 1 cut(s) 279
BstC8I GCNNGC 2 cut(s) 39, 587
BstDEI CTNAG 2 cut(s) 156, 356
BstDSI CCRYGG 1 cut(s) 681
BstF5I GGATG 7 cut(s) 112, 346, 606, 1279, 1349, 1366, 1461
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 2 cut(s) 7, 59
BstKTI GATC 2 cut(s) 112, 1366
BstMAI GTCTC 3 cut(s) 626, 1247, 1443
BstMBI GATC 2 cut(s) 109, 1363
BstMWI GCNNNNNNNGC 3 cut(s) 444, 550, 944
BstNI CCWGG 3 cut(s) 963, 1236, 1473
BstNSI RCATGY 3 cut(s) 544, 1169, 1354
BstSCI CCNGG 5 cut(s) 62, 961, 1093, 1234, 1471
BstSFI CTRYAG 1 cut(s) 445
BstSLI GKGCMC 1 cut(s) 1145
BstV1I GCAGC 2 cut(s) 431, 934
BstV2I GAAGAC 2 cut(s) 1109, 1464
BstXI CCANNNNNNTGG 2 cut(s) 696, 1383
BsuRI GGCC 2 cut(s) 966, 1016
BtgI CCRYGG 1 cut(s) 681
BtgZI GCGATG 1 cut(s) 636
BtsCI GGATG 7 cut(s) 112, 346, 606, 1279, 1349, 1366, 1461
BtsI GCAGTG 1 cut(s) 777
BtsIMutI CAGTG 3 cut(s) 554, 777, 820
BveI ACCTGC 1 cut(s) 438
Cac8I GCNNGC 2 cut(s) 39, 587
CaiI CAGNNNCTG 1 cut(s) 553
CciI TCATGA 1 cut(s) 831
CfoI GCGC 2 cut(s) 7, 59
Cfr13I GGNCC 3 cut(s) 337, 678, 810
CseI GACGC 2 cut(s) 413, 470
Csp6I GTAC 6 cut(s) 433, 451, 514, 802, 1287, 1387
CviQI GTAC 6 cut(s) 433, 451, 514, 802, 1287, 1387
DdeI CTNAG 2 cut(s) 156, 356
DpnI GATC 2 cut(s) 111, 1365
DpnII GATC 2 cut(s) 109, 1363
DriI GACNNNNNGTC 1 cut(s) 283
Eam1104I CTCTTC 5 cut(s) 237, 355, 1020, 1471, 1509
Eam1105I GACNNNNNGTC 1 cut(s) 283
EarI CTCTTC 5 cut(s) 237, 355, 1020, 1471, 1509
EciI GGCGGA 1 cut(s) 24
Eco130I CCWWGG 3 cut(s) 350, 998, 1329
Eco24I GRGCYC 1 cut(s) 997
Eco31I GGTCTC 2 cut(s) 1247, 1443
Eco47I GGWCC 3 cut(s) 337, 678, 810
Eco88I CYCGRG 1 cut(s) 1250
EcoRII CCWGG 3 cut(s) 961, 1234, 1471
EcoT14I CCWWGG 3 cut(s) 350, 998, 1329
EcoT22I ATGCAT 1 cut(s) 252
EcoT38I GRGCYC 1 cut(s) 997
ErhI CCWWGG 3 cut(s) 350, 998, 1329
FaqI GGGAC 2 cut(s) 668, 691
FauI CCCGC 4 cut(s) 46, 431, 666, 1214
FbaI TGATCA 1 cut(s) 109
Fnu4HI GCNGC 2 cut(s) 445, 948
FokI GGATG 7 cut(s) 99, 333, 613, 1286, 1356, 1373, 1448
FriOI GRGCYC 1 cut(s) 997
Fsp4HI GCNGC 2 cut(s) 445, 948
FspBI CTAG 6 cut(s) 84, 152, 485, 636, 846, 999
GlaI GCGC 2 cut(s) 6, 58
GluI GCNGC 2 cut(s) 445, 948
GsaI CCCAGC 1 cut(s) 57
GsuI CTGGAG 1 cut(s) 575
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 2 cut(s) 966, 1016
HapII CCGG 2 cut(s) 64, 1094
HgaI GACGC 2 cut(s) 413, 470
HhaI GCGC 2 cut(s) 7, 59
Hin1I GRCGYC 1 cut(s) 279
Hin6I GCGC 2 cut(s) 5, 57
HinP1I GCGC 2 cut(s) 5, 57
HincII GTYRAC 1 cut(s) 574
HindII GTYRAC 1 cut(s) 574
HinfI GANTC 4 cut(s) 229, 419, 1044, 1114
HpaII CCGG 2 cut(s) 64, 1094
HphI GGTGA 5 cut(s) 170, 392, 887, 1345, 1461
Hpy166II GTNNAC 3 cut(s) 574, 1159, 1336
Hpy188I TCNGA 9 cut(s) 80, 273, 289, 430, 694, 823, 992, 1086, 1534
Hpy188III TCNNGA 6 cut(s) 95, 485, 832, 1048, 1252, 1315
Hpy8I GTNNAC 3 cut(s) 574, 1159, 1336
HpyAV CCTTC 6 cut(s) 185, 433, 997, 1027, 1271, 1476
HpyCH4III ACNGT 3 cut(s) 149, 549, 682
HpyCH4IV ACGT 2 cut(s) 279, 398
HpyCH4V TGCA 9 cut(s) 22, 250, 389, 447, 524, 712, 818, 920, 1522
HpyF10VI GCNNNNNNNGC 3 cut(s) 444, 550, 944
HpyF3I CTNAG 2 cut(s) 156, 356
HpySE526I ACGT 2 cut(s) 279, 398
Hsp92I GRCGYC 1 cut(s) 279
HspAI GCGC 2 cut(s) 5, 57
KpnI GGTACC 1 cut(s) 1390
Ksp22I TGATCA 1 cut(s) 109
Kzo9I GATC 2 cut(s) 109, 1363
LguI GCTCTTC 2 cut(s) 1020, 1509
LmnI GCTCC 5 cut(s) 441, 582, 724, 992, 1172
Lsp1109I GCAGC 2 cut(s) 431, 934
LweI GCATC 4 cut(s) 9, 237, 733, 827
MaeI CTAG 6 cut(s) 84, 152, 485, 636, 846, 999
MaeII ACGT 2 cut(s) 279, 398
MaeIII GTNAC 4 cut(s) 561, 788, 841, 1346
MalI GATC 2 cut(s) 111, 1365
MbiI CCGCTC 1 cut(s) 41
MboI GATC 2 cut(s) 109, 1363
MfeI CAATTG 2 cut(s) 729, 1391
MhlI GDGCHC 2 cut(s) 997, 1145
MluCI AATT 8 cut(s) 223, 719, 729, 956, 981, 1178, 1191, 1391
MlyI GAGTC 2 cut(s) 1053, 1123
MmeI TCCRAC 2 cut(s) 985, 1150
Mph1103I ATGCAT 1 cut(s) 252
MseI TTAA 4 cut(s) 213, 1068, 1437, 1564
MslI CAYNNNNRTG 4 cut(s) 539, 662, 694, 1355
MspI CCGG 2 cut(s) 64, 1094
MspR9I CCNGG 5 cut(s) 64, 963, 1095, 1236, 1473
MunI CAATTG 2 cut(s) 729, 1391
Mva1269I GAATGC 2 cut(s) 250, 589
MvaI CCWGG 3 cut(s) 963, 1236, 1473
MwoI GCNNNNNNNGC 3 cut(s) 444, 550, 944
NciI CCSGG 2 cut(s) 64, 1095
NdeII GATC 2 cut(s) 109, 1363
NlaIV GGNNCC 8 cut(s) 339, 679, 687, 726, 764, 994, 1083, 1388
NmuCI GTSAC 3 cut(s) 561, 841, 1346
NsiI ATGCAT 1 cut(s) 252
NspI RCATGY 3 cut(s) 544, 1169, 1354
PaeR7I CTCGAG 1 cut(s) 1250
PagI TCATGA 1 cut(s) 831
PciI ACATGT 2 cut(s) 1165, 1350
PciSI GCTCTTC 2 cut(s) 1020, 1509
PcsI WCGNNNNNNNCGW 1 cut(s) 285
PctI GAATGC 2 cut(s) 250, 589
PfeI GAWTC 2 cut(s) 229, 419
PkrI GCNGC 2 cut(s) 446, 949
PleI GAGTC 2 cut(s) 1052, 1122
PpsI GAGTC 2 cut(s) 1052, 1122
PscI ACATGT 2 cut(s) 1165, 1350
Psp6I CCWGG 3 cut(s) 961, 1234, 1471
PspFI CCCAGC 1 cut(s) 53
PspGI CCWGG 3 cut(s) 961, 1234, 1471
PspN4I GGNNCC 8 cut(s) 339, 679, 687, 726, 764, 994, 1083, 1388
PspPI GGNCC 3 cut(s) 337, 678, 810
PstI CTGCAG 1 cut(s) 449
PstNI CAGNNNCTG 1 cut(s) 553
RsaI GTAC 6 cut(s) 434, 452, 515, 803, 1288, 1388
RsaNI GTAC 6 cut(s) 433, 451, 514, 802, 1287, 1387
RseI CAYNNNNRTG 4 cut(s) 539, 662, 694, 1355
SapI GCTCTTC 2 cut(s) 1020, 1509
SaqAI TTAA 4 cut(s) 213, 1068, 1437, 1564
SatI GCNGC 2 cut(s) 445, 948
Sau3AI GATC 2 cut(s) 109, 1363
Sau96I GGNCC 3 cut(s) 337, 678, 810
ScaI AGTACT 1 cut(s) 434
SchI GAGTC 2 cut(s) 1053, 1123
ScrFI CCNGG 5 cut(s) 64, 963, 1095, 1236, 1473
SduI GDGCHC 2 cut(s) 997, 1145
SfaNI GCATC 4 cut(s) 9, 237, 733, 827
SfcI CTRYAG 1 cut(s) 445
Sfr274I CTCGAG 1 cut(s) 1250
SinI GGWCC 3 cut(s) 337, 678, 810
SlaI CTCGAG 1 cut(s) 1250
SmiMI CAYNNNNRTG 4 cut(s) 539, 662, 694, 1355
SmlI CTYRAG 3 cut(s) 42, 1211, 1250
SmoI CTYRAG 3 cut(s) 42, 1211, 1250
Sse9I AATT 8 cut(s) 223, 719, 729, 956, 981, 1178, 1191, 1391
SsiI CCGC 5 cut(s) 35, 39, 438, 673, 1221
SspMI CTAG 6 cut(s) 84, 152, 485, 636, 846, 999
StyD4I CCNGG 5 cut(s) 62, 961, 1093, 1234, 1471
StyI CCWWGG 3 cut(s) 350, 998, 1329
TaaI ACNGT 3 cut(s) 149, 549, 682
TaiI ACGT 2 cut(s) 282, 401
TaqI TCGA 5 cut(s) 411, 417, 537, 1188, 1251
TasI AATT 8 cut(s) 223, 719, 729, 956, 981, 1178, 1191, 1391
TatI WGTACW 3 cut(s) 432, 513, 1286
TfiI GAWTC 2 cut(s) 229, 419
Tru1I TTAA 4 cut(s) 213, 1068, 1437, 1564
Tru9I TTAA 4 cut(s) 213, 1068, 1437, 1564
TscAI CASTG 3 cut(s) 554, 784, 820
TseFI GTSAC 3 cut(s) 561, 841, 1346
TseI GCWGC 2 cut(s) 444, 947
Tsp45I GTSAC 3 cut(s) 561, 841, 1346
TspDTI ATGAA 4 cut(s) 848, 957, 1232, 1253
TspRI CASTG 3 cut(s) 554, 784, 820
VpaK11BI GGWCC 3 cut(s) 337, 678, 810
XapI RAATTY 1 cut(s) 956
XbaI TCTAGA 1 cut(s) 484
XceI RCATGY 3 cut(s) 544, 1169, 1354
XhoI CTCGAG 1 cut(s) 1250
XmaJI CCTAGG 1 cut(s) 998
XspI CTAG 6 cut(s) 84, 152, 485, 636, 846, 999
ZraI GACGTC 1 cut(s) 280
ZrmI AGTACT 1 cut(s) 434
Zsp2I ATGCAT 1 cut(s) 252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.