pycom05g25860

Phospholipase A1-Igamma1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0009590
Physical Location & Seq
Reverse (-)
27184521 .. 27186080
1560 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g25860.1

Sequence Viewer

Length: 1560 bp
ATGGCGCTATCAACTATGATGTACACCCATCTTCCGGCCGCTCAAGCCGCCCCCGTTGCAACCCGGTTTTCCTTAATCCGAGCTAGTCATCATCAAGAAGCTGTTCTTGACCATCCCCATCAAACCAAGACATCAAACAAAGCCAACCGTCTAGCTGAATCCCTAGCCAACCTCCTTCACCTCCACATAGATACTCCTCAAAGAACCAACATTAACCCTAATAATTGGCATCTGTTCACAGAAGAGATGCATTCAACTCCCACAACTTCTCCGAAAGACATCATATCCGAAAAATGGCGCAAAATCCACGGGTCCAATGATTGGGAGGGTCTTTTGGACCCTCTCCACCCTTGGCTAAGAAGGGAGATTGTCAAATATGGTGAGTTTGCACAAGCAACGTATGACGCTTTCGATTTCGATGCCTTCTCCGAGTACTGTGGGAGTTGCAGGTACGACAAAAACAAGCTTTTTGATGTGTTGGGTCTAGACAAAAATGGCTACAATGTTAGCAAATACATTTATGCAATGTCACACATCGACATGCCACAGTGGTTGGAGAGGTCACACTTGGCTGACACATGGAGCAAGCATTCCAACTGGATGGGGTATGTTGCCGTGAGCGATGAGGAGGAGACTAGGAGGATTGGGAGGAGGGACATTGTGGTGGCGTGGCGGGGGACCGTGGCGCCATCAGAGTGGTATGAGGATATACAAAGGAAATTGGAGCCAATTGGGAGTGGAGATGCCAAAGTGGAACATGGTTTCCATGGGATTTACACTGCCAAGAGTGACACCACAAGGTACAACAGGTCTAGTGCATCAGAGCAAGTCATGAAAGAAGTGGCTAGGCTCGTGGAGTTGTACCAAGCAAGAGGTGAAGAAGTGAGCCTTACAATCACAGGGCATAGCTTGGGAGGTGCATTGGCTTTGCTCAATGCCTATGAAGCTGCCGAAAAACTCCCTGGCCTTCCGATAAGCGTAATTTCCTTTGGCGCCCCTAGGGTGGGAAACATTGCCTTCAAGGAAGAGCTACACCAAATGGGAGTCAAGACATTGAGGGTTGTGGTTAAACAAGACATGGTTCCGAAAATGCCAGGCCTTGTTTTGAATGAGAGTCTTCAGAAATTTGATGATATTACGGGACCATTGGATTGGGTTTACACACATGTTGGAGCTGAATTGAAGCTCGAAGTTGGTTCTTCGCCTTATCTCAAGCGTGGCGGGTTCAATCTGCCCGGGTTTCATAGCCTCGAGACATACCTCCATCTTGTGGATGGGTTTTTTAGTACAGAAACTACTTTTAGATCAAATGCTAGGAGGGATATTGCCTTGGTGAACAAGGGATGCGACATGTTGGTGGATGATCTTAAAATCCCGCAGTGTTGGTATCAATTGCCACACAAGGGGCTAGTAAGAAACGCTCATGGGAGATGGGTTAAACCAAAACGAGACCCCGAAGACATACCTTCACCTACAAGAGAAGCACAAGCTCATGCTCTTCAAGTAGAGATGATGCAACCATCATCAGATGATACACTTAAATCTTTACGTAGTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

520

Amino Acids

58.76

Weight (kDa)

6.47

Isoelectric Point (pI)

44.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 221 - 367 2.1e-33 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 438
AccB1I GGYRCC 2 cut(s) 685, 992
AccB7I CCANNNNNTGG 2 cut(s) 321, 1270
AccBSI CCGCTC 1 cut(s) 41
AciI CCGC 5 cut(s) 39, 48, 673, 1221, 1376
AcoI YGGCCR 1 cut(s) 36
AcsI RAATTY 1 cut(s) 1124
AcuI CTGAAG 1 cut(s) 1103
AcyI GRCGYC 2 cut(s) 686, 993
AfaI GTAC 6 cut(s) 23, 434, 452, 803, 863, 1288
AfiI CCNNNNNNNGG 7 cut(s) 34, 294, 321, 1003, 1004, 1270, 1403
AflIII ACRYGT 2 cut(s) 1165, 1350
AgsI TTSAA 6 cut(s) 255, 1021, 1108, 1183, 1228, 1502
AjnI CCWGG 2 cut(s) 961, 1093
AloI GAACNNNNNNTCC 2 cut(s) 747, 779
Alw26I GTCTC 3 cut(s) 626, 1247, 1443
Ama87I CYCGRG 2 cut(s) 1235, 1250
AoxI GGCC 3 cut(s) 36, 964, 1096
ApeKI GCWGC 1 cut(s) 947
ApoI RAATTY 1 cut(s) 1124
Asp700I GAANNNNTTC 1 cut(s) 102
AspA2I CCTAGG 1 cut(s) 998
AspLEI GCGC 4 cut(s) 7, 300, 688, 995
AspS9I GGNCC 4 cut(s) 312, 337, 678, 1142
AsuC2I CCSGG 3 cut(s) 64, 1236, 1237
AsuHPI GGTGA 5 cut(s) 170, 392, 887, 1345, 1461
AvaI CYCGRG 2 cut(s) 1235, 1250
AvaII GGWCC 4 cut(s) 312, 337, 678, 1142
AvrII CCTAGG 1 cut(s) 998
BanI GGYRCC 2 cut(s) 685, 992
BauI CACGAG 1 cut(s) 851
BbsI GAAGAC 2 cut(s) 1109, 1464
BbvI GCAGC 1 cut(s) 934
BccI CCATC 9 cut(s) 36, 120, 126, 595, 697, 1268, 1272, 1425, 1528
BceAI ACGGC 1 cut(s) 599
BcgI CGANNNNNNTGC 2 cut(s) 401, 435
BciT130I CCWGG 2 cut(s) 963, 1095
BcnI CCSGG 3 cut(s) 64, 1236, 1237
BcoDI GTCTC 3 cut(s) 626, 1247, 1443
BfoI RGCGCY 3 cut(s) 8, 689, 996
BfuAI ACCTGC 1 cut(s) 438
BisI GCNGC 3 cut(s) 39, 48, 948
BlnI CCTAGG 1 cut(s) 998
BlsI GCNGC 3 cut(s) 40, 49, 949
BmcAI AGTACT 1 cut(s) 434
Bme1390I CCNGG 5 cut(s) 64, 963, 1095, 1236, 1237
Bme18I GGWCC 4 cut(s) 312, 337, 678, 1142
BmeT110I CYCGRG 2 cut(s) 1235, 1250
BmgT120I GGNCC 4 cut(s) 312, 337, 678, 1142
BmiI GGNNCC 8 cut(s) 313, 339, 679, 687, 726, 994, 1083, 1143
BmrFI CCNGG 5 cut(s) 64, 963, 1095, 1236, 1237
BmsI GCATC 7 cut(s) 237, 238, 409, 733, 827, 1334, 1503
BpiI GAAGAC 2 cut(s) 1109, 1464
BpuEI CTTGAG 2 cut(s) 27, 1196
BpuMI CCSGG 3 cut(s) 64, 1236, 1237
BsaAI YACGTR 1 cut(s) 1550
BsaHI GRCGYC 2 cut(s) 686, 993
BsaI GGTCTC 1 cut(s) 1443
BsaJI CCNNGG 8 cut(s) 307, 350, 681, 766, 961, 998, 1235, 1329
BsaXI ACNNNNNCTCC 2 cut(s) 253, 283
Bsc4I CCNNNNNNNGG 7 cut(s) 34, 294, 321, 1003, 1004, 1270, 1403
Bse1I ACTGG 1 cut(s) 602
Bse3DI GCAATG 2 cut(s) 531, 1011
BseBI CCWGG 2 cut(s) 963, 1095
BseDI CCNNGG 8 cut(s) 307, 350, 681, 766, 961, 998, 1235, 1329
BseGI GGATG 5 cut(s) 112, 606, 1279, 1349, 1366
BseLI CCNNNNNNNGG 7 cut(s) 34, 294, 321, 1003, 1004, 1270, 1403
BseMI GCAATG 2 cut(s) 531, 1011
BseNI ACTGG 1 cut(s) 602
BseRI GAGGAG 4 cut(s) 186, 641, 644, 664
BseX3I CGGCCG 1 cut(s) 36
BseXI GCAGC 1 cut(s) 934
Bsh1285I CGRYCG 1 cut(s) 39
BshFI GGCC 3 cut(s) 38, 966, 1098
BshNI GGYRCC 2 cut(s) 685, 992
BsiEI CGRYCG 1 cut(s) 39
BsiHKCI CYCGRG 2 cut(s) 1235, 1250
BsiSI CCGG 3 cut(s) 35, 64, 1236
BslFI GGGAC 3 cut(s) 668, 691, 1155
BslI CCNNNNNNNGG 7 cut(s) 34, 294, 321, 1003, 1004, 1270, 1403
BsmAI GTCTC 3 cut(s) 626, 1247, 1443
BsmFI GGGAC 3 cut(s) 668, 691, 1155
BsmI GAATGC 2 cut(s) 250, 589
BsnI GGCC 3 cut(s) 38, 966, 1098
Bso31I GGTCTC 1 cut(s) 1443
BsoBI CYCGRG 2 cut(s) 1235, 1250
Bsp1407I TGTACA 1 cut(s) 21
Bsp143I GATC 2 cut(s) 1304, 1363
Bsp19I CCATGG 1 cut(s) 766
BspACI CCGC 5 cut(s) 39, 48, 673, 1221, 1376
BspANI GGCC 3 cut(s) 38, 966, 1098
BspHI TCATGA 1 cut(s) 831
BspLI GGNNCC 8 cut(s) 313, 339, 679, 687, 726, 994, 1083, 1143
BspMI ACCTGC 1 cut(s) 438
BspQI GCTCTTC 2 cut(s) 1020, 1503
BspT107I GGYRCC 2 cut(s) 685, 992
BspTNI GGTCTC 1 cut(s) 1443
BsrBI CCGCTC 1 cut(s) 41
BsrDI GCAATG 2 cut(s) 531, 1011
BsrGI TGTACA 1 cut(s) 21
BsrI ACTGG 1 cut(s) 602
BssECI CCNNGG 8 cut(s) 307, 350, 681, 766, 961, 998, 1235, 1329
BssMI GATC 2 cut(s) 1304, 1363
BssNI GRCGYC 2 cut(s) 686, 993
BssSI CACGAG 1 cut(s) 851
BssT1I CCWWGG 4 cut(s) 350, 766, 998, 1329
Bst2BI CACGAG 1 cut(s) 851
Bst2UI CCWGG 2 cut(s) 963, 1095
Bst4CI ACNGT 4 cut(s) 149, 437, 549, 682
Bst6I CTCTTC 3 cut(s) 237, 1020, 1503
BstACI GRCGYC 2 cut(s) 686, 993
BstAUI TGTACA 1 cut(s) 21
BstBAI YACGTR 1 cut(s) 1550
BstC8I GCNNGC 1 cut(s) 587
BstDEI CTNAG 1 cut(s) 356
BstDSI CCRYGG 3 cut(s) 307, 681, 766
BstF5I GGATG 5 cut(s) 112, 606, 1279, 1349, 1366
BstH2I RGCGCY 3 cut(s) 8, 689, 996
BstHHI GCGC 4 cut(s) 7, 300, 688, 995
BstKTI GATC 2 cut(s) 1307, 1366
BstMAI GTCTC 3 cut(s) 626, 1247, 1443
BstMBI GATC 2 cut(s) 1304, 1363
BstMCI CGRYCG 1 cut(s) 39
BstMWI GCNNNNNNNGC 4 cut(s) 44, 47, 56, 944
BstNI CCWGG 2 cut(s) 963, 1095
BstNSI RCATGY 3 cut(s) 544, 1169, 1354
BstSCI CCNGG 5 cut(s) 62, 961, 1093, 1234, 1235
BstSNI TACGTA 1 cut(s) 1550
BstV1I GCAGC 1 cut(s) 934
BstV2I GAAGAC 2 cut(s) 1109, 1464
BstXI CCANNNNNNTGG 3 cut(s) 601, 696, 1152
BstZI CGGCCG 1 cut(s) 36
BsuRI GGCC 3 cut(s) 38, 966, 1098
BtgI CCRYGG 3 cut(s) 307, 681, 766
BtgZI GCGATG 1 cut(s) 636
BtsCI GGATG 5 cut(s) 112, 606, 1279, 1349, 1366
BtsI GCAGTG 2 cut(s) 777, 1385
BtsIMutI CAGTG 3 cut(s) 554, 777, 1385
BveI ACCTGC 1 cut(s) 438
Cac8I GCNNGC 1 cut(s) 587
CciI TCATGA 1 cut(s) 831
CfoI GCGC 4 cut(s) 7, 300, 688, 995
Cfr13I GGNCC 4 cut(s) 312, 337, 678, 1142
Cfr9I CCCGGG 1 cut(s) 1235
CseI GACGC 1 cut(s) 413
Csp6I GTAC 6 cut(s) 22, 433, 451, 802, 862, 1287
CspCI CAANNNNNGTGG 2 cut(s) 534, 569
CviQI GTAC 6 cut(s) 22, 433, 451, 802, 862, 1287
DdeI CTNAG 1 cut(s) 356
DinI GGCGCC 2 cut(s) 687, 994
DpnI GATC 2 cut(s) 1306, 1365
DpnII GATC 2 cut(s) 1304, 1363
EaeI YGGCCR 1 cut(s) 36
EagI CGGCCG 1 cut(s) 36
Eam1104I CTCTTC 3 cut(s) 237, 1020, 1503
EarI CTCTTC 3 cut(s) 237, 1020, 1503
EclXI CGGCCG 1 cut(s) 36
Eco105I TACGTA 1 cut(s) 1550
Eco130I CCWWGG 4 cut(s) 350, 766, 998, 1329
Eco147I AGGCCT 1 cut(s) 1098
Eco31I GGTCTC 1 cut(s) 1443
Eco47I GGWCC 4 cut(s) 312, 337, 678, 1142
Eco52I CGGCCG 1 cut(s) 36
Eco57I CTGAAG 1 cut(s) 1103
Eco88I CYCGRG 2 cut(s) 1235, 1250
EcoRII CCWGG 2 cut(s) 961, 1093
EcoT14I CCWWGG 4 cut(s) 350, 766, 998, 1329
EcoT22I ATGCAT 1 cut(s) 252
EgeI GGCGCC 2 cut(s) 687, 994
EheI GGCGCC 2 cut(s) 687, 994
ErhI CCWWGG 4 cut(s) 350, 766, 998, 1329
FalI AAGNNNNNCTT 4 cut(s) 90, 122, 873, 905
FaqI GGGAC 3 cut(s) 668, 691, 1155
FauI CCCGC 3 cut(s) 666, 1214, 1383
Fnu4HI GCNGC 3 cut(s) 39, 48, 948
FokI GGATG 5 cut(s) 99, 613, 1286, 1356, 1373
Fsp4HI GCNGC 3 cut(s) 39, 48, 948
GlaI GCGC 4 cut(s) 6, 299, 687, 994
GluI GCNGC 3 cut(s) 39, 48, 948
HaeII RGCGCY 3 cut(s) 8, 689, 996
HaeIII GGCC 3 cut(s) 38, 966, 1098
HapII CCGG 3 cut(s) 35, 64, 1236
HgaI GACGC 1 cut(s) 413
HhaI GCGC 4 cut(s) 7, 300, 688, 995
Hin1I GRCGYC 2 cut(s) 686, 993
Hin6I GCGC 4 cut(s) 5, 298, 686, 993
HinP1I GCGC 4 cut(s) 5, 298, 686, 993
HindIII AAGCTT 1 cut(s) 464
HinfI GANTC 3 cut(s) 158, 1044, 1114
HpaII CCGG 3 cut(s) 35, 64, 1236
HphI GGTGA 5 cut(s) 170, 392, 887, 1345, 1461
Hpy166II GTNNAC 4 cut(s) 24, 237, 1159, 1336
Hpy188III TCNNGA 6 cut(s) 95, 107, 485, 832, 1048, 1252
Hpy8I GTNNAC 4 cut(s) 24, 237, 1159, 1336
HpyAV CCTTC 6 cut(s) 185, 354, 433, 977, 1027, 1476
HpyCH4III ACNGT 4 cut(s) 149, 437, 549, 682
HpyCH4IV ACGT 2 cut(s) 398, 1549
HpyCH4V TGCA 8 cut(s) 59, 250, 389, 447, 524, 818, 920, 1516
HpyF10VI GCNNNNNNNGC 4 cut(s) 44, 47, 56, 944
HpyF3I CTNAG 1 cut(s) 356
HpySE526I ACGT 2 cut(s) 398, 1549
Hsp92I GRCGYC 2 cut(s) 686, 993
HspAI GCGC 4 cut(s) 5, 298, 686, 993
KasI GGCGCC 2 cut(s) 685, 992
Kzo9I GATC 2 cut(s) 1304, 1363
LguI GCTCTTC 2 cut(s) 1020, 1503
LmnI GCTCC 3 cut(s) 582, 724, 1172
Lsp1109I GCAGC 1 cut(s) 934
LweI GCATC 7 cut(s) 237, 238, 409, 733, 827, 1334, 1503
MaeII ACGT 2 cut(s) 398, 1549
MaeIII GTNAC 3 cut(s) 528, 561, 788
MalI GATC 2 cut(s) 1306, 1365
MbiI CCGCTC 1 cut(s) 41
MboI GATC 2 cut(s) 1304, 1363
MboII GAAGA 8 cut(s) 23, 254, 890, 1037, 1109, 1191, 1469, 1490
MfeI CAATTG 2 cut(s) 729, 1391
MluCI AATT 7 cut(s) 223, 719, 729, 981, 1124, 1178, 1391
Mly113I GGCGCC 2 cut(s) 686, 993
MlyI GAGTC 2 cut(s) 1053, 1123
MmeI TCCRAC 3 cut(s) 534, 618, 1150
Mph1103I ATGCAT 1 cut(s) 252
MroXI GAANNNNTTC 1 cut(s) 102
MseI TTAA 7 cut(s) 74, 213, 1068, 1368, 1437, 1539, 1558
MslI CAYNNNNRTG 4 cut(s) 539, 662, 694, 1355
MspI CCGG 3 cut(s) 35, 64, 1236
MspR9I CCNGG 5 cut(s) 64, 963, 1095, 1236, 1237
MunI CAATTG 2 cut(s) 729, 1391
Mva1269I GAATGC 2 cut(s) 250, 589
MvaI CCWGG 2 cut(s) 963, 1095
MwoI GCNNNNNNNGC 4 cut(s) 44, 47, 56, 944
NarI GGCGCC 2 cut(s) 686, 993
NciI CCSGG 3 cut(s) 64, 1236, 1237
NcoI CCATGG 1 cut(s) 766
NdeII GATC 2 cut(s) 1304, 1363
NlaIV GGNNCC 8 cut(s) 313, 339, 679, 687, 726, 994, 1083, 1143
NmuCI GTSAC 3 cut(s) 528, 561, 788
NsiI ATGCAT 1 cut(s) 252
NspI RCATGY 3 cut(s) 544, 1169, 1354
PaeR7I CTCGAG 1 cut(s) 1250
PagI TCATGA 1 cut(s) 831
PceI AGGCCT 1 cut(s) 1098
PciI ACATGT 2 cut(s) 1165, 1350
PciSI GCTCTTC 2 cut(s) 1020, 1503
PctI GAATGC 2 cut(s) 250, 589
PdmI GAANNNNTTC 1 cut(s) 102
PfeI GAWTC 1 cut(s) 158
PflMI CCANNNNNTGG 2 cut(s) 321, 1270
PkrI GCNGC 3 cut(s) 40, 49, 949
PleI GAGTC 2 cut(s) 1052, 1122
PluTI GGCGCC 2 cut(s) 689, 996
PpsI GAGTC 2 cut(s) 1052, 1122
Ppu21I YACGTR 1 cut(s) 1550
PscI ACATGT 2 cut(s) 1165, 1350
Psp6I CCWGG 2 cut(s) 961, 1093
PspGI CCWGG 2 cut(s) 961, 1093
PspN4I GGNNCC 8 cut(s) 313, 339, 679, 687, 726, 994, 1083, 1143
PspPI GGNCC 4 cut(s) 312, 337, 678, 1142
RsaI GTAC 6 cut(s) 23, 434, 452, 803, 863, 1288
RsaNI GTAC 6 cut(s) 22, 433, 451, 802, 862, 1287
RseI CAYNNNNRTG 4 cut(s) 539, 662, 694, 1355
SapI GCTCTTC 2 cut(s) 1020, 1503
SaqAI TTAA 7 cut(s) 74, 213, 1068, 1368, 1437, 1539, 1558
SatI GCNGC 3 cut(s) 39, 48, 948
Sau3AI GATC 2 cut(s) 1304, 1363
Sau96I GGNCC 4 cut(s) 312, 337, 678, 1142
ScaI AGTACT 1 cut(s) 434
SchI GAGTC 2 cut(s) 1053, 1123
ScrFI CCNGG 5 cut(s) 64, 963, 1095, 1236, 1237
SfaNI GCATC 7 cut(s) 237, 238, 409, 733, 827, 1334, 1503
SfoI GGCGCC 2 cut(s) 687, 994
Sfr274I CTCGAG 1 cut(s) 1250
SinI GGWCC 4 cut(s) 312, 337, 678, 1142
SlaI CTCGAG 1 cut(s) 1250
SmaI CCCGGG 1 cut(s) 1237
SmiMI CAYNNNNRTG 4 cut(s) 539, 662, 694, 1355
SmlI CTYRAG 3 cut(s) 42, 1211, 1250
SmoI CTYRAG 3 cut(s) 42, 1211, 1250
SnaBI TACGTA 1 cut(s) 1550
Sse9I AATT 7 cut(s) 223, 719, 729, 981, 1124, 1178, 1391
SseBI AGGCCT 1 cut(s) 1098
SsiI CCGC 5 cut(s) 39, 48, 673, 1221, 1376
SspDI GGCGCC 2 cut(s) 685, 992
StuI AGGCCT 1 cut(s) 1098
StyD4I CCNGG 5 cut(s) 62, 961, 1093, 1234, 1235
StyI CCWWGG 4 cut(s) 350, 766, 998, 1329
TaaI ACNGT 4 cut(s) 149, 437, 549, 682
TaiI ACGT 2 cut(s) 401, 1552
TaqI TCGA 5 cut(s) 411, 417, 537, 1188, 1251
TasI AATT 7 cut(s) 223, 719, 729, 981, 1124, 1178, 1391
TatI WGTACW 3 cut(s) 21, 432, 1286
TauI GCSGC 2 cut(s) 41, 50
TfiI GAWTC 1 cut(s) 158
Tru1I TTAA 7 cut(s) 74, 213, 1068, 1368, 1437, 1539, 1558
Tru9I TTAA 7 cut(s) 74, 213, 1068, 1368, 1437, 1539, 1558
TscAI CASTG 3 cut(s) 554, 784, 1385
TseFI GTSAC 3 cut(s) 528, 561, 788
TseI GCWGC 1 cut(s) 947
Tsp45I GTSAC 3 cut(s) 528, 561, 788
TspDTI ATGAA 3 cut(s) 848, 957, 1232
TspMI CCCGGG 1 cut(s) 1235
TspRI CASTG 3 cut(s) 554, 784, 1385
Van91I CCANNNNNTGG 2 cut(s) 321, 1270
VpaK11BI GGWCC 4 cut(s) 312, 337, 678, 1142
XapI RAATTY 1 cut(s) 1124
XbaI TCTAGA 1 cut(s) 484
XceI RCATGY 3 cut(s) 544, 1169, 1354
XcmI CCANNNNNNNNNTGG 1 cut(s) 1271
XhoI CTCGAG 1 cut(s) 1250
XmaI CCCGGG 1 cut(s) 1235
XmaJI CCTAGG 1 cut(s) 998
XmnI GAANNNNTTC 1 cut(s) 102
ZrmI AGTACT 1 cut(s) 434
Zsp2I ATGCAT 1 cut(s) 252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.