MD05G1278100.v1.1

Phospholipase A1-Igamma1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0009590
Physical Location & Seq
Reverse (-)
41261927 .. 41263474
1548 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1278100.v1.1.491

Sequence Viewer

Length: 1548 bp
ATGTACACCCATCTTCCGGCCGCTCAAGCCACTCCCAGCGCAACCCGGTTTTCCCAAATCCGAGCTAGTCATCATCAAGAAGCTGATTTTGATCATCCCCATCAAACCAAGACATCAAACAAAGTCAACCGTCTAGCTAAATCCGTTGCCAACCTCCTTCACCTCCACATAGATATTCCTCAAAGAACCAACATTAACCCTAATAATTGGAATCTGCTCATAGAAGAGATGCATTCAACTCCCACGACTTCTCCGAAAGACATCATGTCCGAAAAATGGCGCGAAATCCACGGGTCAAATGATTGGGAGGGTCTTTTGGACCCTCTCCACCCTTGGCTAAGAAGAGAGATTGTCAAATATGGTGAGTTTGCACAAGCAACGTATGACGCTTTCGATTTCGATTCCTTCTCCGAGTACTGCGGGAGCTGCAGGTACAACAAAAACAAGCTCTTTGATGTGTTGGGTCTAGACAAAAATGGCTACAATGTTAGCAAGTACATTTATGCAATGTCACACATCGACATGCCACAGTGGCTGGAGAGGTCACACTTGGCTGACACATGGAGCAAGCATTCTAACTGGATGGGGTATGTTGCTGTGAGCGATGATGAGGAGACTAGGAGGATTGGGAGGAGGGACATTGTGGTGGCGTGGCGGGGGACCGTGGTGCCATCAGAGTGGTATGAGGATATGCAAAGGAAATTGGAGCCAATTGGGAGTGGAGATGCCAAAGTGGAACATGGTTTCCATGGGATTTACACTGCCAAGAGTGACACCACAAGGTACAACAAGTCTAGTGCATCAGAGCAAGTCATGAAAGAAGTGACTAGGCTTGTGGAGTTGTACCAAGCAAAAGGTGAAGAAGTGAGCCTAACAATCACAGGGCATAGCTTGGGAGGTGCATTGGCTTTGCTCAATGCCTATGAAGCTGCCGAAAAACTCCCTGGCATTATGATAAGCGTAATTTCCTTTGGCGCCCCTAGGGTTGGAAACATTGCCTTCAAGGAAGAGCTACACCAAATGGGAGTCAAGACATTGAGGGTTGTGGTTAAACAAGACATGGTTCCGAAAATGCCAGGGCTTGTTTTGAATGAGAGTCTTCAAAAGTTTGATGACATTACGGGCACTTTGGATTGGGTTTACGCACATGTTGGAGCTGAATTGAAGCTCGAAATTGGTTCTTCGCCTTATCTCAAGCATGGTGGGTTCAATCTGCCTGGCTTTCATAGCCTCGAGACCTACCTTCATCTTGTGGATGGGTTTTTGAGTACAGAAACTACTTTTAGGTCAAATGCTAGAAGGGATATTACCTTGGTGAACAAGGGATGTGACATGTTGGTGGATGATCTCAGAATCCCACAATGTTGGTACCAATTGCCACACAAGGGGTTAGTAAGAAACGTTCATGGGAGATGGGTTAAACCGAAACGAGACCCCGAAGACATCCCTTCACCTGGAAGAGAAGCACAAGCACAAGCTCATGCTCTTCAAGTAGAGATGGTGCAATTGTCATCAGATTATACACACAAATCTTTATGTAGTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

516

Amino Acids

58.45

Weight (kDa)

6.41

Isoelectric Point (pI)

45.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 215 - 361 2e-33 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 420
Acc65I GGTACC 1 cut(s) 1368
AccB1I GGYRCC 3 cut(s) 667, 974, 1368
AccBSI CCGCTC 1 cut(s) 23
AccII CGCG 1 cut(s) 282
AciI CCGC 3 cut(s) 21, 420, 655
AclI AACGTT 1 cut(s) 1401
AcoI YGGCCR 1 cut(s) 18
AcyI GRCGYC 1 cut(s) 975
AfaI GTAC 8 cut(s) 5, 416, 434, 497, 785, 845, 1270, 1370
AfiI CCNNNNNNNGG 5 cut(s) 16, 276, 986, 1385, 1454
AflIII ACRYGT 2 cut(s) 1147, 1332
AgsI TTSAA 7 cut(s) 237, 1003, 1090, 1103, 1165, 1210, 1490
AhdI GACNNNNNGTC 1 cut(s) 265
AjnI CCWGG 4 cut(s) 943, 1075, 1216, 1453
AloI GAACNNNNNNTCC 2 cut(s) 729, 761
Alw26I GTCTC 3 cut(s) 608, 1229, 1425
AlwNI CAGNNNCTG 1 cut(s) 535
Ama87I CYCGRG 1 cut(s) 1232
AoxI GGCC 1 cut(s) 18
ApeKI GCWGC 2 cut(s) 426, 929
Asp718I GGTACC 1 cut(s) 1368
AspA2I CCTAGG 1 cut(s) 980
AspLEI GCGC 3 cut(s) 41, 282, 977
AspS9I GGNCC 2 cut(s) 319, 660
AsuC2I CCSGG 1 cut(s) 46
AsuHPI GGTGA 5 cut(s) 152, 374, 869, 1327, 1443
AvaI CYCGRG 1 cut(s) 1232
AvaII GGWCC 2 cut(s) 319, 660
AvrII CCTAGG 1 cut(s) 980
BaeGI GKGCMC 1 cut(s) 1127
BanI GGYRCC 3 cut(s) 667, 974, 1368
BarI GAAGNNNNNNTAC 2 cut(s) 1291, 1323
BbsI GAAGAC 2 cut(s) 1091, 1446
BbvI GCAGC 2 cut(s) 413, 916
BccI CCATC 7 cut(s) 18, 108, 577, 679, 1250, 1407, 1492
BciT130I CCWGG 4 cut(s) 945, 1077, 1218, 1455
BclI TGATCA 1 cut(s) 91
BcnI CCSGG 1 cut(s) 46
BcoDI GTCTC 3 cut(s) 608, 1229, 1425
BfaI CTAG 8 cut(s) 66, 134, 467, 618, 795, 828, 981, 1296
BfmI CTRYAG 1 cut(s) 427
BfoI RGCGCY 1 cut(s) 978
BfuAI ACCTGC 1 cut(s) 420
BglI GCCNNNNNGGC 1 cut(s) 532
BisI GCNGC 3 cut(s) 21, 427, 930
BlnI CCTAGG 1 cut(s) 980
BlsI GCNGC 3 cut(s) 22, 428, 931
BmcAI AGTACT 1 cut(s) 416
Bme1390I CCNGG 5 cut(s) 46, 945, 1077, 1218, 1455
Bme18I GGWCC 2 cut(s) 319, 660
BmeRI GACNNNNNGTC 1 cut(s) 265
BmeT110I CYCGRG 1 cut(s) 1232
BmgT120I GGNCC 2 cut(s) 319, 660
BmiI GGNNCC 7 cut(s) 321, 661, 669, 708, 976, 1065, 1370
BmrFI CCNGG 5 cut(s) 46, 945, 1077, 1218, 1455
BmsI GCATC 3 cut(s) 219, 715, 809
BpiI GAAGAC 2 cut(s) 1091, 1446
BpmI CTGGAG 1 cut(s) 557
BpuEI CTTGAG 2 cut(s) 9, 1178
BpuMI CCSGG 1 cut(s) 46
BsaBI GATNNNNATC 1 cut(s) 90
BsaHI GRCGYC 1 cut(s) 975
BsaI GGTCTC 2 cut(s) 1229, 1425
BsaJI CCNNGG 8 cut(s) 289, 332, 663, 748, 943, 980, 1076, 1311
BsaXI ACNNNNNCTCC 2 cut(s) 235, 265
Bsc4I CCNNNNNNNGG 5 cut(s) 16, 276, 986, 1385, 1454
Bse1I ACTGG 1 cut(s) 584
Bse3DI GCAATG 2 cut(s) 513, 993
Bse8I GATNNNNATC 1 cut(s) 90
BseBI CCWGG 4 cut(s) 945, 1077, 1218, 1455
BseDI CCNNGG 8 cut(s) 289, 332, 663, 748, 943, 980, 1076, 1311
BseGI GGATG 6 cut(s) 94, 588, 1261, 1331, 1348, 1443
BseJI GATNNNNATC 1 cut(s) 90
BseLI CCNNNNNNNGG 5 cut(s) 16, 276, 986, 1385, 1454
BseMI GCAATG 2 cut(s) 513, 993
BseMII CTCAG 1 cut(s) 1363
BseNI ACTGG 1 cut(s) 584
BseRI GAGGAG 2 cut(s) 626, 646
BseSI GKGCMC 1 cut(s) 1127
BseX3I CGGCCG 1 cut(s) 18
BseXI GCAGC 2 cut(s) 413, 916
BseYI CCCAGC 1 cut(s) 35
Bsh1236I CGCG 1 cut(s) 282
Bsh1285I CGRYCG 1 cut(s) 21
BshFI GGCC 1 cut(s) 20
BshNI GGYRCC 3 cut(s) 667, 974, 1368
BsiEI CGRYCG 1 cut(s) 21
BsiHKCI CYCGRG 1 cut(s) 1232
BsiSI CCGG 2 cut(s) 17, 46
BslFI GGGAC 2 cut(s) 650, 673
BslI CCNNNNNNNGG 5 cut(s) 16, 276, 986, 1385, 1454
BsmAI GTCTC 3 cut(s) 608, 1229, 1425
BsmFI GGGAC 2 cut(s) 650, 673
BsmI GAATGC 2 cut(s) 232, 571
BsnI GGCC 1 cut(s) 20
Bso31I GGTCTC 2 cut(s) 1229, 1425
BsoBI CYCGRG 1 cut(s) 1232
Bsp1286I GDGCHC 1 cut(s) 1127
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 2 cut(s) 91, 1345
Bsp19I CCATGG 1 cut(s) 748
BspACI CCGC 3 cut(s) 21, 420, 655
BspANI GGCC 1 cut(s) 20
BspCNI CTCAG 1 cut(s) 1362
BspFNI CGCG 1 cut(s) 282
BspHI TCATGA 1 cut(s) 813
BspLI GGNNCC 7 cut(s) 321, 661, 669, 708, 976, 1065, 1370
BspMAI CTGCAG 1 cut(s) 431
BspMI ACCTGC 1 cut(s) 420
BspQI GCTCTTC 2 cut(s) 1002, 1491
BspT107I GGYRCC 3 cut(s) 667, 974, 1368
BspTNI GGTCTC 2 cut(s) 1229, 1425
BsrBI CCGCTC 1 cut(s) 23
BsrDI GCAATG 2 cut(s) 513, 993
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 1 cut(s) 584
BssECI CCNNGG 8 cut(s) 289, 332, 663, 748, 943, 980, 1076, 1311
BssMI GATC 2 cut(s) 91, 1345
BssNI GRCGYC 1 cut(s) 975
BssT1I CCWWGG 4 cut(s) 332, 748, 980, 1311
Bst2UI CCWGG 4 cut(s) 945, 1077, 1218, 1455
Bst4CI ACNGT 3 cut(s) 131, 531, 664
Bst6I CTCTTC 5 cut(s) 219, 337, 1002, 1453, 1491
BstACI GRCGYC 1 cut(s) 975
BstAUI TGTACA 1 cut(s) 3
BstC8I GCNNGC 1 cut(s) 569
BstDEI CTNAG 2 cut(s) 338, 1349
BstDSI CCRYGG 3 cut(s) 289, 663, 748
BstF5I GGATG 6 cut(s) 94, 588, 1261, 1331, 1348, 1443
BstFNI CGCG 1 cut(s) 282
BstH2I RGCGCY 1 cut(s) 978
BstHHI GCGC 3 cut(s) 41, 282, 977
BstKTI GATC 2 cut(s) 94, 1348
BstMAI GTCTC 3 cut(s) 608, 1229, 1425
BstMBI GATC 2 cut(s) 91, 1345
BstMCI CGRYCG 1 cut(s) 21
BstMWI GCNNNNNNNGC 5 cut(s) 26, 426, 532, 926, 1227
BstNI CCWGG 4 cut(s) 945, 1077, 1218, 1455
BstNSI RCATGY 3 cut(s) 526, 1151, 1336
BstSCI CCNGG 5 cut(s) 44, 943, 1075, 1216, 1453
BstSFI CTRYAG 1 cut(s) 427
BstSLI GKGCMC 1 cut(s) 1127
BstUI CGCG 1 cut(s) 282
BstV1I GCAGC 2 cut(s) 413, 916
BstV2I GAAGAC 2 cut(s) 1091, 1446
BstXI CCANNNNNNTGG 2 cut(s) 678, 1365
BstZI CGGCCG 1 cut(s) 18
BsuRI GGCC 1 cut(s) 20
BtgI CCRYGG 3 cut(s) 289, 663, 748
BtgZI GCGATG 1 cut(s) 618
BtsCI GGATG 6 cut(s) 94, 588, 1261, 1331, 1348, 1443
BtsI GCAGTG 1 cut(s) 759
BtsIMutI CAGTG 2 cut(s) 536, 759
BveI ACCTGC 1 cut(s) 420
Cac8I GCNNGC 1 cut(s) 569
CaiI CAGNNNCTG 1 cut(s) 535
CciI TCATGA 1 cut(s) 813
CfoI GCGC 3 cut(s) 41, 282, 977
Cfr13I GGNCC 2 cut(s) 319, 660
CseI GACGC 1 cut(s) 395
Csp6I GTAC 8 cut(s) 4, 415, 433, 496, 784, 844, 1269, 1369
CspCI CAANNNNNGTGG 2 cut(s) 1183, 1218
CviQI GTAC 8 cut(s) 4, 415, 433, 496, 784, 844, 1269, 1369
DdeI CTNAG 2 cut(s) 338, 1349
DinI GGCGCC 1 cut(s) 976
DpnI GATC 2 cut(s) 93, 1347
DpnII GATC 2 cut(s) 91, 1345
DriI GACNNNNNGTC 1 cut(s) 265
EaeI YGGCCR 1 cut(s) 18
EagI CGGCCG 1 cut(s) 18
Eam1104I CTCTTC 5 cut(s) 219, 337, 1002, 1453, 1491
Eam1105I GACNNNNNGTC 1 cut(s) 265
EarI CTCTTC 5 cut(s) 219, 337, 1002, 1453, 1491
EclXI CGGCCG 1 cut(s) 18
Eco130I CCWWGG 4 cut(s) 332, 748, 980, 1311
Eco31I GGTCTC 2 cut(s) 1229, 1425
Eco47I GGWCC 2 cut(s) 319, 660
Eco52I CGGCCG 1 cut(s) 18
Eco88I CYCGRG 1 cut(s) 1232
EcoRII CCWGG 4 cut(s) 943, 1075, 1216, 1453
EcoT14I CCWWGG 4 cut(s) 332, 748, 980, 1311
EcoT22I ATGCAT 1 cut(s) 234
EgeI GGCGCC 1 cut(s) 976
EheI GGCGCC 1 cut(s) 976
ErhI CCWWGG 4 cut(s) 332, 748, 980, 1311
FaqI GGGAC 2 cut(s) 650, 673
FauI CCCGC 2 cut(s) 413, 648
FbaI TGATCA 1 cut(s) 91
Fnu4HI GCNGC 3 cut(s) 21, 427, 930
FokI GGATG 6 cut(s) 81, 595, 1268, 1338, 1355, 1430
Fsp4HI GCNGC 3 cut(s) 21, 427, 930
FspBI CTAG 8 cut(s) 66, 134, 467, 618, 795, 828, 981, 1296
GlaI GCGC 3 cut(s) 40, 281, 976
GluI GCNGC 3 cut(s) 21, 427, 930
GsaI CCCAGC 1 cut(s) 39
GsuI CTGGAG 1 cut(s) 557
HaeII RGCGCY 1 cut(s) 978
HaeIII GGCC 1 cut(s) 20
HapII CCGG 2 cut(s) 17, 46
HgaI GACGC 1 cut(s) 395
HhaI GCGC 3 cut(s) 41, 282, 977
Hin1I GRCGYC 1 cut(s) 975
Hin6I GCGC 3 cut(s) 39, 280, 975
HinP1I GCGC 3 cut(s) 39, 280, 975
HincII GTYRAC 1 cut(s) 127
HindII GTYRAC 1 cut(s) 127
HinfI GANTC 5 cut(s) 211, 401, 1026, 1096, 1353
HpaII CCGG 2 cut(s) 17, 46
HphI GGTGA 5 cut(s) 152, 374, 869, 1327, 1443
Hpy166II GTNNAC 4 cut(s) 6, 127, 1141, 1318
Hpy188I TCNGA 9 cut(s) 62, 255, 271, 412, 676, 805, 1068, 1352, 1516
Hpy188III TCNNGA 5 cut(s) 77, 467, 814, 1030, 1234
Hpy8I GTNNAC 4 cut(s) 6, 127, 1141, 1318
HpyAV CCTTC 6 cut(s) 167, 415, 1009, 1253, 1293, 1458
HpyCH4III ACNGT 3 cut(s) 131, 531, 664
HpyCH4IV ACGT 2 cut(s) 380, 1401
HpyCH4V TGCA 8 cut(s) 232, 371, 429, 506, 694, 800, 902, 1504
HpyF10VI GCNNNNNNNGC 5 cut(s) 26, 426, 532, 926, 1227
HpyF3I CTNAG 2 cut(s) 338, 1349
HpySE526I ACGT 2 cut(s) 380, 1401
Hsp92I GRCGYC 1 cut(s) 975
HspAI GCGC 3 cut(s) 39, 280, 975
KasI GGCGCC 1 cut(s) 974
KpnI GGTACC 1 cut(s) 1372
Ksp22I TGATCA 1 cut(s) 91
Kzo9I GATC 2 cut(s) 91, 1345
LguI GCTCTTC 2 cut(s) 1002, 1491
LmnI GCTCC 4 cut(s) 423, 564, 706, 1154
Lsp1109I GCAGC 2 cut(s) 413, 916
LweI GCATC 3 cut(s) 219, 715, 809
MaeI CTAG 8 cut(s) 66, 134, 467, 618, 795, 828, 981, 1296
MaeII ACGT 2 cut(s) 380, 1401
MaeIII GTNAC 5 cut(s) 510, 543, 770, 823, 1328
MalI GATC 2 cut(s) 93, 1347
MbiI CCGCTC 1 cut(s) 23
MboI GATC 2 cut(s) 91, 1345
MfeI CAATTG 3 cut(s) 711, 1373, 1505
MhlI GDGCHC 1 cut(s) 1127
MluCI AATT 8 cut(s) 205, 701, 711, 963, 1160, 1173, 1373, 1505
Mly113I GGCGCC 1 cut(s) 975
MlyI GAGTC 2 cut(s) 1035, 1105
MmeI TCCRAC 2 cut(s) 967, 1132
Mph1103I ATGCAT 1 cut(s) 234
MseI TTAA 4 cut(s) 195, 1050, 1419, 1546
MslI CAYNNNNRTG 4 cut(s) 521, 644, 676, 1337
MspI CCGG 2 cut(s) 17, 46
MspR9I CCNGG 5 cut(s) 46, 945, 1077, 1218, 1455
MunI CAATTG 3 cut(s) 711, 1373, 1505
Mva1269I GAATGC 2 cut(s) 232, 571
MvaI CCWGG 4 cut(s) 945, 1077, 1218, 1455
MvnI CGCG 1 cut(s) 282
MwoI GCNNNNNNNGC 5 cut(s) 26, 426, 532, 926, 1227
NarI GGCGCC 1 cut(s) 975
NciI CCSGG 1 cut(s) 46
NcoI CCATGG 1 cut(s) 748
NdeII GATC 2 cut(s) 91, 1345
NlaIV GGNNCC 7 cut(s) 321, 661, 669, 708, 976, 1065, 1370
NmuCI GTSAC 5 cut(s) 510, 543, 770, 823, 1328
NsiI ATGCAT 1 cut(s) 234
NspI RCATGY 3 cut(s) 526, 1151, 1336
PaeR7I CTCGAG 1 cut(s) 1232
PagI TCATGA 1 cut(s) 813
PciI ACATGT 2 cut(s) 1147, 1332
PciSI GCTCTTC 2 cut(s) 1002, 1491
PcsI WCGNNNNNNNCGW 1 cut(s) 251
PctI GAATGC 2 cut(s) 232, 571
PfeI GAWTC 3 cut(s) 211, 401, 1353
PkrI GCNGC 3 cut(s) 22, 428, 931
PleI GAGTC 2 cut(s) 1034, 1104
PluTI GGCGCC 1 cut(s) 978
PpsI GAGTC 2 cut(s) 1034, 1104
PscI ACATGT 2 cut(s) 1147, 1332
Psp1406I AACGTT 1 cut(s) 1401
Psp6I CCWGG 4 cut(s) 943, 1075, 1216, 1453
PspFI CCCAGC 1 cut(s) 35
PspGI CCWGG 4 cut(s) 943, 1075, 1216, 1453
PspN4I GGNNCC 7 cut(s) 321, 661, 669, 708, 976, 1065, 1370
PspPI GGNCC 2 cut(s) 319, 660
PsrI GAACNNNNNNTAC 2 cut(s) 1386, 1418
PstI CTGCAG 1 cut(s) 431
PstNI CAGNNNCTG 1 cut(s) 535
RsaI GTAC 8 cut(s) 5, 416, 434, 497, 785, 845, 1270, 1370
RsaNI GTAC 8 cut(s) 4, 415, 433, 496, 784, 844, 1269, 1369
RseI CAYNNNNRTG 4 cut(s) 521, 644, 676, 1337
SapI GCTCTTC 2 cut(s) 1002, 1491
SaqAI TTAA 4 cut(s) 195, 1050, 1419, 1546
SatI GCNGC 3 cut(s) 21, 427, 930
Sau3AI GATC 2 cut(s) 91, 1345
Sau96I GGNCC 2 cut(s) 319, 660
ScaI AGTACT 1 cut(s) 416
SchI GAGTC 2 cut(s) 1035, 1105
ScrFI CCNGG 5 cut(s) 46, 945, 1077, 1218, 1455
SduI GDGCHC 1 cut(s) 1127
SfaNI GCATC 3 cut(s) 219, 715, 809
SfcI CTRYAG 1 cut(s) 427
SfoI GGCGCC 1 cut(s) 976
Sfr274I CTCGAG 1 cut(s) 1232
SinI GGWCC 2 cut(s) 319, 660
SlaI CTCGAG 1 cut(s) 1232
SmiMI CAYNNNNRTG 4 cut(s) 521, 644, 676, 1337
SmlI CTYRAG 3 cut(s) 24, 1193, 1232
SmoI CTYRAG 3 cut(s) 24, 1193, 1232
Sse9I AATT 8 cut(s) 205, 701, 711, 963, 1160, 1173, 1373, 1505
SsiI CCGC 3 cut(s) 21, 420, 655
SspDI GGCGCC 1 cut(s) 974
SspMI CTAG 8 cut(s) 66, 134, 467, 618, 795, 828, 981, 1296
StyD4I CCNGG 5 cut(s) 44, 943, 1075, 1216, 1453
StyI CCWWGG 4 cut(s) 332, 748, 980, 1311
TaaI ACNGT 3 cut(s) 131, 531, 664
TaiI ACGT 2 cut(s) 383, 1404
TaqI TCGA 5 cut(s) 393, 399, 519, 1170, 1233
TasI AATT 8 cut(s) 205, 701, 711, 963, 1160, 1173, 1373, 1505
TatI WGTACW 4 cut(s) 3, 414, 495, 1268
TauI GCSGC 1 cut(s) 23
TfiI GAWTC 3 cut(s) 211, 401, 1353
Tru1I TTAA 4 cut(s) 195, 1050, 1419, 1546
Tru9I TTAA 4 cut(s) 195, 1050, 1419, 1546
TscAI CASTG 2 cut(s) 536, 766
TseFI GTSAC 5 cut(s) 510, 543, 770, 823, 1328
TseI GCWGC 2 cut(s) 426, 929
Tsp45I GTSAC 5 cut(s) 510, 543, 770, 823, 1328
TspDTI ATGAA 5 cut(s) 830, 939, 1214, 1235, 1394
TspGWI ACGGA 1 cut(s) 133
TspRI CASTG 2 cut(s) 536, 766
VpaK11BI GGWCC 2 cut(s) 319, 660
XbaI TCTAGA 1 cut(s) 466
XceI RCATGY 3 cut(s) 526, 1151, 1336
XhoI CTCGAG 1 cut(s) 1232
XmaJI CCTAGG 1 cut(s) 980
XspI CTAG 8 cut(s) 66, 134, 467, 618, 795, 828, 981, 1296
ZrmI AGTACT 1 cut(s) 416
Zsp2I ATGCAT 1 cut(s) 234
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.