pycom05g25890

Phospholipase A1-Igamma1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0009590
Physical Location & Seq
Reverse (-)
27194477 .. 27195805
1329 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g25890.1

Sequence Viewer

Length: 1329 bp
ATGATCACAGAAGAGAGCCATTCAACTCCCACTACTTCTCCGAAAGACAACATATCCGAAAAATGGCGCGAAATCCACGGGTCAAATGATTGGGAGGGTCTTTTGGACCCTCTCCACCCTTGGATAAGAAGAGAGATTGTCAAATATGGTGAGTTTGCACAAGCAACGTATGACGCTTTTGATTTCGATTTCTTCTCCGAGTACTGCGGGAGCTGCAGGTACAACAAAAACAAGCTTTTTGATGTGTTGGGTCTAGACAAAAATGGCTACAATGTTAGCAAGTACATTTATGCAATGTCACACATCGACATGCCGCAGTGGCTGGAGAGGTCACACTTGGCTGACACATGGAGCAAGCATTCCAACTGGATGGGGTATGTTGCCGTGAGCGACGAGGAGGAGACTAGGAGGATTGGGAGGAGGGACATTGTGGTGGCGTGGCGGGGGACCGTGGTGCCATCGGAGTGGTATGAGGATATGCAAAGGAAATTGGAGCCAATTGGGAGTGGAGATGCCAAAGTGGAACATGGGTTCCATGGGATTTACACTGCCAAGAGTGACACCACAAGGTACAACAAGTCTAGTGCATCAGAGCAAGTCATGAAAGAAGTGACTAGGCTTGTGGAGTTGTACCAAGCAAAAGGTGAAGAAGTGAGCCTTACAATCACAGGGCATAGCTTGGGAGGTGCATTGGCTTTGGTCAATGCCTATGAAGCTGCCGAAAAACTCCCTGGCCTTCCGATAAGCGTAATTTCCTTTGGCGCCCCTAGGGTTGGAAACATTGCCTTCAAGGAAGAGCTACACCAAATGGGAGTCAAGACATTGAGAGTTGTGGTTAAACAAGACATGGTTCCGAAAATGCCGGGGCTTGTTTTGAATGAAAGTCTTCAGAAGTTTGATGACATTACGGGCACATTGGATTGGGTTTACACACATGTTGGGGCTGAATTGAAGCTCGAAGTTGGTTTTTCGCCTTATCTCAAGCGTGGTGGGTTCAATCTGCCAGGGTTTCATAGCCTCGAGACCTACCTCCATCTTGTGGATGGGTTTTTGAGTACAGAAACTACTTTTAGGTCAAATGCTAGGAGGGATATTGCCTTGGTGAACAAGGGATGTGACATGTTGGTGGATGATCTTAGAATTCCACAATGTTGGTACCAATTGCCACACAAGGGGTTAGTAAGAAACGCTCATGGGAGATGGGTTAAACCGAAACGAGACCCTGAAGACATACCTTCACCTGCAAGAGAAGCACAAGCGCAAGCTCATGCTCTTCAATTAGAGATGATGCAACCATCATCAGATTATATACTTGAATCTTTTATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

443

Amino Acids

50.3

Weight (kDa)

5.84

Isoelectric Point (pI)

45.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 144 - 290 5.9e-33 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1249
Acc36I ACCTGC 2 cut(s) 207, 1249
Acc65I GGTACC 1 cut(s) 1155
AccB1I GGYRCC 3 cut(s) 454, 761, 1155
AccB7I CCANNNNNTGG 1 cut(s) 1039
AccII CGCG 1 cut(s) 69
AciI CCGC 3 cut(s) 207, 314, 442
AcsI RAATTY 1 cut(s) 1140
AcuI CTGAAG 2 cut(s) 872, 1245
AcyI GRCGYC 1 cut(s) 762
AfaI GTAC 7 cut(s) 203, 221, 284, 572, 632, 1057, 1157
AfiI CCNNNNNNNGG 4 cut(s) 63, 773, 1039, 1172
AflIII ACRYGT 2 cut(s) 934, 1119
AgsI TTSAA 7 cut(s) 24, 790, 877, 952, 997, 1277, 1316
AjnI CCWGG 2 cut(s) 730, 1003
AloI GAACNNNNNNTCC 4 cut(s) 515, 516, 547, 548
AluBI AGCT 7 cut(s) 213, 235, 678, 716, 799, 955, 1265
AluI AGCT 7 cut(s) 213, 235, 678, 716, 799, 955, 1265
Alw26I GTCTC 3 cut(s) 395, 1016, 1212
AlwNI CAGNNNCTG 1 cut(s) 322
Ama87I CYCGRG 1 cut(s) 1019
AoxI GGCC 1 cut(s) 733
ApeKI GCWGC 2 cut(s) 213, 716
ApoI RAATTY 1 cut(s) 1140
Asp700I GAANNNNTTC 1 cut(s) 885
Asp718I GGTACC 1 cut(s) 1155
AspA2I CCTAGG 1 cut(s) 767
AspLEI GCGC 3 cut(s) 69, 764, 1261
AspS9I GGNCC 2 cut(s) 106, 447
AsuC2I CCSGG 1 cut(s) 864
AsuHPI GGTGA 4 cut(s) 161, 656, 1114, 1230
AvaI CYCGRG 1 cut(s) 1019
AvaII GGWCC 2 cut(s) 106, 447
AvrII CCTAGG 1 cut(s) 767
BaeGI GKGCMC 1 cut(s) 914
BanI GGYRCC 3 cut(s) 454, 761, 1155
BbsI GAAGAC 2 cut(s) 878, 1233
BbvI GCAGC 2 cut(s) 200, 703
BccI CCATC 6 cut(s) 364, 466, 1037, 1041, 1194, 1303
BceAI ACGGC 1 cut(s) 368
BciT130I CCWGG 2 cut(s) 732, 1005
BclI TGATCA 1 cut(s) 3
BcnI CCSGG 1 cut(s) 864
BcoDI GTCTC 3 cut(s) 395, 1016, 1212
BfaI CTAG 6 cut(s) 254, 405, 582, 615, 768, 1083
BfmI CTRYAG 1 cut(s) 214
BfoI RGCGCY 1 cut(s) 765
BfuAI ACCTGC 2 cut(s) 207, 1249
BglI GCCNNNNNGGC 1 cut(s) 319
BisI GCNGC 3 cut(s) 214, 314, 717
BlnI CCTAGG 1 cut(s) 767
BlsI GCNGC 3 cut(s) 215, 315, 718
BmcAI AGTACT 1 cut(s) 203
Bme1390I CCNGG 3 cut(s) 732, 864, 1005
Bme18I GGWCC 2 cut(s) 106, 447
BmeT110I CYCGRG 1 cut(s) 1019
BmgT120I GGNCC 2 cut(s) 106, 447
BmiI GGNNCC 8 cut(s) 108, 448, 456, 495, 533, 763, 852, 1157
BmrFI CCNGG 3 cut(s) 732, 864, 1005
BmsI GCATC 3 cut(s) 502, 596, 1278
BpiI GAAGAC 2 cut(s) 878, 1233
BpmI CTGGAG 1 cut(s) 344
BpuEI CTTGAG 1 cut(s) 965
BpuMI CCSGG 1 cut(s) 864
BsaHI GRCGYC 1 cut(s) 762
BsaI GGTCTC 2 cut(s) 1016, 1212
BsaJI CCNNGG 9 cut(s) 76, 119, 450, 535, 730, 767, 863, 1004, 1098
BsaXI ACNNNNNCTCC 2 cut(s) 22, 52
Bsc4I CCNNNNNNNGG 4 cut(s) 63, 773, 1039, 1172
Bse1I ACTGG 1 cut(s) 371
Bse3DI GCAATG 2 cut(s) 300, 780
BseBI CCWGG 2 cut(s) 732, 1005
BseDI CCNNGG 9 cut(s) 76, 119, 450, 535, 730, 767, 863, 1004, 1098
BseGI GGATG 4 cut(s) 375, 1048, 1118, 1135
BseLI CCNNNNNNNGG 4 cut(s) 63, 773, 1039, 1172
BseMI GCAATG 2 cut(s) 300, 780
BseNI ACTGG 1 cut(s) 371
BseRI GAGGAG 3 cut(s) 410, 413, 433
BseSI GKGCMC 1 cut(s) 914
BseXI GCAGC 2 cut(s) 200, 703
Bsh1236I CGCG 1 cut(s) 69
BshFI GGCC 1 cut(s) 735
BshNI GGYRCC 3 cut(s) 454, 761, 1155
BsiHKCI CYCGRG 1 cut(s) 1019
BsiSI CCGG 1 cut(s) 863
BslFI GGGAC 2 cut(s) 437, 460
BslI CCNNNNNNNGG 4 cut(s) 63, 773, 1039, 1172
BsmAI GTCTC 3 cut(s) 395, 1016, 1212
BsmFI GGGAC 2 cut(s) 437, 460
BsmI GAATGC 1 cut(s) 358
BsnI GGCC 1 cut(s) 735
Bso31I GGTCTC 2 cut(s) 1016, 1212
BsoBI CYCGRG 1 cut(s) 1019
Bsp1286I GDGCHC 1 cut(s) 914
Bsp143I GATC 2 cut(s) 3, 1132
Bsp19I CCATGG 1 cut(s) 535
BspACI CCGC 3 cut(s) 207, 314, 442
BspANI GGCC 1 cut(s) 735
BspFNI CGCG 1 cut(s) 69
BspHI TCATGA 1 cut(s) 600
BspLI GGNNCC 8 cut(s) 108, 448, 456, 495, 533, 763, 852, 1157
BspMAI CTGCAG 1 cut(s) 218
BspMI ACCTGC 2 cut(s) 207, 1249
BspQI GCTCTTC 2 cut(s) 789, 1278
BspT107I GGYRCC 3 cut(s) 454, 761, 1155
BspTNI GGTCTC 2 cut(s) 1016, 1212
BsrDI GCAATG 2 cut(s) 300, 780
BsrI ACTGG 1 cut(s) 371
BssECI CCNNGG 9 cut(s) 76, 119, 450, 535, 730, 767, 863, 1004, 1098
BssMI GATC 2 cut(s) 3, 1132
BssNI GRCGYC 1 cut(s) 762
BssT1I CCWWGG 4 cut(s) 119, 535, 767, 1098
Bst2UI CCWGG 2 cut(s) 732, 1005
Bst4CI ACNGT 1 cut(s) 451
Bst6I CTCTTC 4 cut(s) 6, 124, 789, 1278
BstACI GRCGYC 1 cut(s) 762
BstC8I GCNNGC 2 cut(s) 356, 1263
BstDEI CTNAG 1 cut(s) 1136
BstDSI CCRYGG 3 cut(s) 76, 450, 535
BstF5I GGATG 4 cut(s) 375, 1048, 1118, 1135
BstFNI CGCG 1 cut(s) 69
BstH2I RGCGCY 1 cut(s) 765
BstHHI GCGC 3 cut(s) 69, 764, 1261
BstKTI GATC 2 cut(s) 6, 1135
BstMAI GTCTC 3 cut(s) 395, 1016, 1212
BstMBI GATC 2 cut(s) 3, 1132
BstMWI GCNNNNNNNGC 4 cut(s) 213, 319, 713, 1250
BstNI CCWGG 2 cut(s) 732, 1005
BstNSI RCATGY 3 cut(s) 313, 938, 1123
BstSCI CCNGG 3 cut(s) 730, 862, 1003
BstSFI CTRYAG 1 cut(s) 214
BstSLI GKGCMC 1 cut(s) 914
BstUI CGCG 1 cut(s) 69
BstV1I GCAGC 2 cut(s) 200, 703
BstV2I GAAGAC 2 cut(s) 878, 1233
BstXI CCANNNNNNTGG 3 cut(s) 370, 465, 1152
BsuRI GGCC 1 cut(s) 735
BtgI CCRYGG 3 cut(s) 76, 450, 535
BtsCI GGATG 4 cut(s) 375, 1048, 1118, 1135
BtsI GCAGTG 2 cut(s) 323, 546
BtsIMutI CAGTG 2 cut(s) 323, 546
BveI ACCTGC 2 cut(s) 207, 1249
Cac8I GCNNGC 2 cut(s) 356, 1263
CaiI CAGNNNCTG 1 cut(s) 322
CciI TCATGA 1 cut(s) 600
CfoI GCGC 3 cut(s) 69, 764, 1261
Cfr13I GGNCC 2 cut(s) 106, 447
CseI GACGC 1 cut(s) 182
Csp6I GTAC 7 cut(s) 202, 220, 283, 571, 631, 1056, 1156
CspCI CAANNNNNGTGG 2 cut(s) 970, 1005
CviQI GTAC 7 cut(s) 202, 220, 283, 571, 631, 1056, 1156
DdeI CTNAG 1 cut(s) 1136
DinI GGCGCC 1 cut(s) 763
DpnI GATC 2 cut(s) 5, 1134
DpnII GATC 2 cut(s) 3, 1132
Eam1104I CTCTTC 4 cut(s) 6, 124, 789, 1278
EarI CTCTTC 4 cut(s) 6, 124, 789, 1278
Eco130I CCWWGG 4 cut(s) 119, 535, 767, 1098
Eco31I GGTCTC 2 cut(s) 1016, 1212
Eco47I GGWCC 2 cut(s) 106, 447
Eco57I CTGAAG 2 cut(s) 872, 1245
Eco88I CYCGRG 1 cut(s) 1019
EcoRI GAATTC 1 cut(s) 1140
EcoRII CCWGG 2 cut(s) 730, 1003
EcoT14I CCWWGG 4 cut(s) 119, 535, 767, 1098
EgeI GGCGCC 1 cut(s) 763
EheI GGCGCC 1 cut(s) 763
ErhI CCWWGG 4 cut(s) 119, 535, 767, 1098
FalI AAGNNNNNCTT 2 cut(s) 642, 674
FaqI GGGAC 2 cut(s) 437, 460
FauI CCCGC 2 cut(s) 200, 435
FbaI TGATCA 1 cut(s) 3
Fnu4HI GCNGC 3 cut(s) 214, 314, 717
FokI GGATG 4 cut(s) 382, 1055, 1125, 1142
Fsp4HI GCNGC 3 cut(s) 214, 314, 717
FspBI CTAG 6 cut(s) 254, 405, 582, 615, 768, 1083
GlaI GCGC 3 cut(s) 68, 763, 1260
GluI GCNGC 3 cut(s) 214, 314, 717
GsuI CTGGAG 1 cut(s) 344
HaeII RGCGCY 1 cut(s) 765
HaeIII GGCC 1 cut(s) 735
HapII CCGG 1 cut(s) 863
HgaI GACGC 1 cut(s) 182
HhaI GCGC 3 cut(s) 69, 764, 1261
Hin1I GRCGYC 1 cut(s) 762
Hin6I GCGC 3 cut(s) 67, 762, 1259
HinP1I GCGC 3 cut(s) 67, 762, 1259
HindIII AAGCTT 1 cut(s) 233
HinfI GANTC 2 cut(s) 813, 1316
HpaII CCGG 1 cut(s) 863
HphI GGTGA 4 cut(s) 161, 656, 1114, 1230
Hpy166II GTNNAC 2 cut(s) 928, 1105
Hpy188I TCNGA 9 cut(s) 42, 58, 199, 463, 592, 741, 855, 891, 1303
Hpy188III TCNNGA 4 cut(s) 254, 601, 817, 1021
Hpy8I GTNNAC 2 cut(s) 928, 1105
Hpy99I CGWCG 1 cut(s) 395
HpyAV CCTTC 3 cut(s) 746, 796, 1245
HpyCH4III ACNGT 1 cut(s) 451
HpyCH4IV ACGT 1 cut(s) 167
HpyCH4V TGCA 8 cut(s) 158, 216, 293, 481, 587, 689, 1244, 1291
HpyF10VI GCNNNNNNNGC 4 cut(s) 213, 319, 713, 1250
HpyF3I CTNAG 1 cut(s) 1136
HpySE526I ACGT 1 cut(s) 167
Hsp92I GRCGYC 1 cut(s) 762
HspAI GCGC 3 cut(s) 67, 762, 1259
KasI GGCGCC 1 cut(s) 761
KpnI GGTACC 1 cut(s) 1159
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 2 cut(s) 3, 1132
LguI GCTCTTC 2 cut(s) 789, 1278
LmnI GCTCC 3 cut(s) 210, 351, 493
Lsp1109I GCAGC 2 cut(s) 200, 703
LweI GCATC 3 cut(s) 502, 596, 1278
MaeI CTAG 6 cut(s) 254, 405, 582, 615, 768, 1083
MaeII ACGT 1 cut(s) 167
MaeIII GTNAC 5 cut(s) 297, 330, 557, 610, 1115
MalI GATC 2 cut(s) 5, 1134
MboI GATC 2 cut(s) 3, 1132
MboII GAAGA 8 cut(s) 23, 141, 184, 659, 806, 878, 1238, 1265
MfeI CAATTG 2 cut(s) 498, 1160
MhlI GDGCHC 1 cut(s) 914
MluCI AATT 7 cut(s) 488, 498, 750, 947, 1140, 1160, 1277
Mly113I GGCGCC 1 cut(s) 762
MlyI GAGTC 1 cut(s) 822
MmeI TCCRAC 2 cut(s) 387, 754
MroXI GAANNNNTTC 1 cut(s) 885
MseI TTAA 2 cut(s) 837, 1206
MslI CAYNNNNRTG 4 cut(s) 308, 431, 463, 1124
MspI CCGG 1 cut(s) 863
MspR9I CCNGG 3 cut(s) 732, 864, 1005
MunI CAATTG 2 cut(s) 498, 1160
Mva1269I GAATGC 1 cut(s) 358
MvaI CCWGG 2 cut(s) 732, 1005
MvnI CGCG 1 cut(s) 69
MwoI GCNNNNNNNGC 4 cut(s) 213, 319, 713, 1250
NarI GGCGCC 1 cut(s) 762
NciI CCSGG 1 cut(s) 864
NcoI CCATGG 1 cut(s) 535
NdeII GATC 2 cut(s) 3, 1132
NlaIV GGNNCC 8 cut(s) 108, 448, 456, 495, 533, 763, 852, 1157
NmuCI GTSAC 5 cut(s) 297, 330, 557, 610, 1115
NspI RCATGY 3 cut(s) 313, 938, 1123
PaeR7I CTCGAG 1 cut(s) 1019
PagI TCATGA 1 cut(s) 600
PaqCI CACCTGC 1 cut(s) 1249
PciI ACATGT 2 cut(s) 934, 1119
PciSI GCTCTTC 2 cut(s) 789, 1278
PctI GAATGC 1 cut(s) 358
PdmI GAANNNNTTC 1 cut(s) 885
PfeI GAWTC 1 cut(s) 1316
PflMI CCANNNNNTGG 1 cut(s) 1039
PkrI GCNGC 3 cut(s) 215, 315, 718
PleI GAGTC 1 cut(s) 821
PluTI GGCGCC 1 cut(s) 765
PpsI GAGTC 1 cut(s) 821
PscI ACATGT 2 cut(s) 934, 1119
Psp6I CCWGG 2 cut(s) 730, 1003
PspGI CCWGG 2 cut(s) 730, 1003
PspN4I GGNNCC 8 cut(s) 108, 448, 456, 495, 533, 763, 852, 1157
PspPI GGNCC 2 cut(s) 106, 447
PstI CTGCAG 1 cut(s) 218
PstNI CAGNNNCTG 1 cut(s) 322
RsaI GTAC 7 cut(s) 203, 221, 284, 572, 632, 1057, 1157
RsaNI GTAC 7 cut(s) 202, 220, 283, 571, 631, 1056, 1156
RseI CAYNNNNRTG 4 cut(s) 308, 431, 463, 1124
SapI GCTCTTC 2 cut(s) 789, 1278
SaqAI TTAA 2 cut(s) 837, 1206
SatI GCNGC 3 cut(s) 214, 314, 717
Sau3AI GATC 2 cut(s) 3, 1132
Sau96I GGNCC 2 cut(s) 106, 447
ScaI AGTACT 1 cut(s) 203
SchI GAGTC 1 cut(s) 822
ScrFI CCNGG 3 cut(s) 732, 864, 1005
SduI GDGCHC 1 cut(s) 914
SfaNI GCATC 3 cut(s) 502, 596, 1278
SfcI CTRYAG 1 cut(s) 214
SfoI GGCGCC 1 cut(s) 763
Sfr274I CTCGAG 1 cut(s) 1019
SinI GGWCC 2 cut(s) 106, 447
SlaI CTCGAG 1 cut(s) 1019
SmiMI CAYNNNNRTG 4 cut(s) 308, 431, 463, 1124
SmlI CTYRAG 2 cut(s) 980, 1019
SmoI CTYRAG 2 cut(s) 980, 1019
Sse9I AATT 7 cut(s) 488, 498, 750, 947, 1140, 1160, 1277
SsiI CCGC 3 cut(s) 207, 314, 442
SspDI GGCGCC 1 cut(s) 761
SspMI CTAG 6 cut(s) 254, 405, 582, 615, 768, 1083
StyD4I CCNGG 3 cut(s) 730, 862, 1003
StyI CCWWGG 4 cut(s) 119, 535, 767, 1098
TaaI ACNGT 1 cut(s) 451
TaiI ACGT 1 cut(s) 170
TaqI TCGA 4 cut(s) 186, 306, 957, 1020
TasI AATT 7 cut(s) 488, 498, 750, 947, 1140, 1160, 1277
TatI WGTACW 3 cut(s) 201, 282, 1055
TauI GCSGC 1 cut(s) 316
TfiI GAWTC 1 cut(s) 1316
Tru1I TTAA 2 cut(s) 837, 1206
Tru9I TTAA 2 cut(s) 837, 1206
TscAI CASTG 2 cut(s) 323, 553
TseFI GTSAC 5 cut(s) 297, 330, 557, 610, 1115
TseI GCWGC 2 cut(s) 213, 716
Tsp45I GTSAC 5 cut(s) 297, 330, 557, 610, 1115
TspDTI ATGAA 4 cut(s) 617, 726, 894, 1001
TspRI CASTG 2 cut(s) 323, 553
Van91I CCANNNNNTGG 1 cut(s) 1039
VpaK11BI GGWCC 2 cut(s) 106, 447
XapI RAATTY 1 cut(s) 1140
XbaI TCTAGA 1 cut(s) 253
XceI RCATGY 3 cut(s) 313, 938, 1123
XcmI CCANNNNNNNNNTGG 1 cut(s) 1040
XhoI CTCGAG 1 cut(s) 1019
XmaJI CCTAGG 1 cut(s) 767
XmnI GAANNNNTTC 1 cut(s) 885
XspI CTAG 6 cut(s) 254, 405, 582, 615, 768, 1083
ZrmI AGTACT 1 cut(s) 203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.