Rroxscaffold_1G00061520

Phospholipase A1-Igamma1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0009590
Physical Location & Seq
Reverse (-)
83629606 .. 83631183
1578 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00061520.1

Sequence Viewer

Length: 1578 bp
ATGGCAATGTTAACTATGATGTATGCTCATATTCCGGCCACTCAAGTGGCCTCCGCAGCAACCCGGTTTCCCTTGATCCGATCTCAACAGCACCAAGAAGCTGTTCTTGATCATCACCCACAAACAAAGACATCAAACAGGACCAACCGTCTGGCTGAATCACTATCCCACCTCCTCCATCTTCACATCGAAACCCCTCCTAAAACCAACAGTCCTTACTTTTCCAATTGGAATATGTTTACTGAAGAGAAGCATTCAACTCCCACAACTTCTCCCAAGGAAATCATATCCGAAAGGTGGCGTGAAATCCACGGCTCAAAAGATTGGGAGGGTCTAATTGATCCACTCCACCCTTGGCTAAGAAGAGAGATAGTCAAGTATGGTGAATTTGCACAGGCTACATATGATGCTTTCGACTTTGACTCATTCTCTGAGTTCTGTGGGAGCTGCAGGTACAACAAGAACAAGCTCTTTGATGTTTTGGGACTGGGCAAAAATGGATATGCAGTTAGCAAATACATTTACGCCATGTCCCATATAGACATGCCTAATTGGCTGGAGAGGTCACATTTGGTTGAGACTTGGAGCAAAGATTCAAACTGGATGGGATTTGTTGCAGTGAGTGATGACGAGGAGACGACAAGGATCGGAAGGAGAGATATTGCGGTGGCTTGGCGTGGGACGGTGGCGCCATCCGAATGGTATGAGGATCTGCAGAGGAAGTTGGAACCTATTGGAGATTTGGATGCCAAAGTTGAACATGGGTTTCTTGGGATTTACAAGTCCAAGAGTGAGAAAACCAGATACAACAAATCTAGTGCCTCAGAGCAAGTCATGAAGGAAGTGATTAGGCTTGTTGAGTTTTATAAATCCAAGGGTGAAGAAGTGAGTCTTACAATTACAGGGCATAGCTTGGGTGGTGCTTTGGCTTTGCTCAATGCTTATGAAGCTGCAAAATCAATTCCTGGGCTACCCATTAGTGTCATTTCATTTGGGGCACCTAGAGTGGGAAACAATGCTTTCAGAGATGAGCTAAACCAAATGGGGGTCAAGACACTGAGGGTTGTGGTCAAGCAAGACGTGGTTCCGAAAATGCCGGGACTTGTTTTGAATGAAGGTTTGCAGAAGTTTGAAGAAATTACAGGCACATTGGAATGGGTTTATACACATGTTGGAGCTGAACTGAAAGTTGATGTCGGCTCGTCGCCTTATCTCAAGAAACATGGGTTCAATTTTCTAGGGTTTCATAGCCTTGAGACTTATCTTCACCTTGTGGATGGGTATTTGAATAAAGAAACTAGCTTTAGGTCAAATGCCAGGAGGGATTTTGCCTTGGTGAACAAGGCATGTGACATGTTGGTGGAGGATCTTAGAATCCCACATTGTTGGTACCAATTGGAACACAAGGGACTTGTTTGTAATCATCATGGGAGATGGGTTAAACCCATAAGAGAGCCTGAAGACATTCCTTCACCTACTAGAGAAGCAGAAGCACAAGCTCATGCTTTTCAAGTAGAGATGCAATCAGATTATGTACTTCAATCTTTATGTACTGCTAATATGAAAGTTGAATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

525

Amino Acids

59.91

Weight (kDa)

6.38

Isoelectric Point (pI)

47.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 223 - 368 9.1e-33 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 867
Acc36I ACCTGC 1 cut(s) 441
Acc65I GGTACC 1 cut(s) 1389
AccB1I GGYRCC 3 cut(s) 688, 997, 1389
AciI CCGC 2 cut(s) 54, 665
AclWI GGATC 5 cut(s) 70, 335, 653, 717, 1374
AcoI YGGCCR 1 cut(s) 36
AcsI RAATTY 2 cut(s) 386, 1571
AcuI CTGAAG 2 cut(s) 264, 1479
AcyI GRCGYC 1 cut(s) 689
AdeI CACNNNGTG 1 cut(s) 1273
AfaI GTAC 4 cut(s) 455, 1391, 1536, 1552
AfiI CCNNNNNNNGG 3 cut(s) 297, 1007, 1045
AflIII ACRYGT 2 cut(s) 1168, 1353
AhdI GACNNNNNGTC 1 cut(s) 147
AjiI CACGTC 1 cut(s) 1081
AjnI CCWGG 2 cut(s) 964, 1316
AjuI GAANNNNNNNTTGG 2 cut(s) 87, 119
AleI CACNNNNGTG 1 cut(s) 44
AluBI AGCT 9 cut(s) 101, 447, 469, 912, 950, 1033, 1178, 1302, 1499
AluI AGCT 9 cut(s) 101, 447, 469, 912, 950, 1033, 1178, 1302, 1499
Alw26I GTCTC 3 cut(s) 572, 629, 1250
AlwI GGATC 5 cut(s) 70, 335, 653, 717, 1374
AoxI GGCC 2 cut(s) 36, 48
ApeKI GCWGC 3 cut(s) 56, 447, 950
ApoI RAATTY 2 cut(s) 386, 1571
Asp700I GAANNNNTTC 2 cut(s) 102, 1464
Asp718I GGTACC 1 cut(s) 1389
AspLEI GCGC 1 cut(s) 691
AspS9I GGNCC 1 cut(s) 141
AsuC2I CCSGG 2 cut(s) 64, 1098
AsuHPI GGTGA 6 cut(s) 107, 395, 890, 1259, 1348, 1464
AvaII GGWCC 1 cut(s) 141
BaeGI GKGCMC 1 cut(s) 1000
BanI GGYRCC 3 cut(s) 688, 997, 1389
BbsI GAAGAC 1 cut(s) 1467
BbvI GCAGC 3 cut(s) 68, 434, 937
BccI CCATC 5 cut(s) 186, 598, 700, 1271, 1428
BceAI ACGGC 1 cut(s) 328
BciT130I CCWGG 2 cut(s) 966, 1318
BclI TGATCA 1 cut(s) 109
BcnI CCSGG 2 cut(s) 64, 1098
BcoDI GTCTC 3 cut(s) 572, 629, 1250
BfaI CTAG 5 cut(s) 816, 1002, 1238, 1299, 1479
BfmI CTRYAG 2 cut(s) 448, 713
BfoI RGCGCY 1 cut(s) 692
BfuAI ACCTGC 1 cut(s) 441
BglI GCCNNNNNGGC 1 cut(s) 553
BisI GCNGC 3 cut(s) 57, 448, 951
BlsI GCNGC 3 cut(s) 58, 449, 952
Bme1390I CCNGG 4 cut(s) 64, 966, 1098, 1318
Bme18I GGWCC 1 cut(s) 141
BmeRI GACNNNNNGTC 1 cut(s) 147
BmgBI CACGTC 1 cut(s) 1081
BmgT120I GGNCC 1 cut(s) 141
BmiI GGNNCC 5 cut(s) 690, 729, 999, 1086, 1391
BmrFI CCNGG 4 cut(s) 64, 966, 1098, 1318
BmrI ACTGGG 1 cut(s) 497
BmsI GCATC 3 cut(s) 397, 736, 1509
BmuI ACTGGG 1 cut(s) 497
BpiI GAAGAC 1 cut(s) 1467
BpmI CTGGAG 1 cut(s) 578
BpuEI CTTGAG 3 cut(s) 27, 1199, 1274
BpuMI CCSGG 2 cut(s) 64, 1098
BsaHI GRCGYC 1 cut(s) 689
BsaJI CCNNGG 6 cut(s) 276, 310, 353, 873, 965, 1332
BsaXI ACNNNNNCTCC 2 cut(s) 256, 286
Bsc4I CCNNNNNNNGG 3 cut(s) 297, 1007, 1045
Bse1I ACTGG 2 cut(s) 492, 605
Bse3DI GCAATG 1 cut(s) 12
BseBI CCWGG 2 cut(s) 966, 1318
BseDI CCNNGG 6 cut(s) 276, 310, 353, 873, 965, 1332
BseGI GGATG 4 cut(s) 609, 692, 751, 1282
BseLI CCNNNNNNNGG 3 cut(s) 297, 1007, 1045
BseMI GCAATG 1 cut(s) 12
BseMII CTCAG 3 cut(s) 423, 837, 1049
BseNI ACTGG 2 cut(s) 492, 605
BseRI GAGGAG 2 cut(s) 164, 647
BseSI GKGCMC 1 cut(s) 1000
BseXI GCAGC 3 cut(s) 68, 434, 937
BshFI GGCC 2 cut(s) 38, 50
BshNI GGYRCC 3 cut(s) 688, 997, 1389
BsiSI CCGG 3 cut(s) 35, 64, 1097
BslFI GGGAC 5 cut(s) 498, 517, 694, 1113, 1422
BslI CCNNNNNNNGG 3 cut(s) 297, 1007, 1045
BsmAI GTCTC 3 cut(s) 572, 629, 1250
BsmBI CGTCTC 1 cut(s) 629
BsmFI GGGAC 5 cut(s) 498, 517, 694, 1113, 1422
BsmI GAATGC 1 cut(s) 253
BsnI GGCC 2 cut(s) 38, 50
Bsp1286I GDGCHC 1 cut(s) 1000
Bsp143I GATC 7 cut(s) 75, 80, 109, 340, 645, 709, 1366
BspACI CCGC 2 cut(s) 54, 665
BspANI GGCC 2 cut(s) 38, 50
BspCNI CTCAG 3 cut(s) 424, 836, 1050
BspHI TCATGA 1 cut(s) 834
BspLI GGNNCC 5 cut(s) 690, 729, 999, 1086, 1391
BspMAI CTGCAG 2 cut(s) 452, 717
BspMI ACCTGC 1 cut(s) 441
BspPI GGATC 5 cut(s) 70, 335, 653, 717, 1374
BspT107I GGYRCC 3 cut(s) 688, 997, 1389
BsrDI GCAATG 1 cut(s) 12
BsrI ACTGG 2 cut(s) 492, 605
BssECI CCNNGG 6 cut(s) 276, 310, 353, 873, 965, 1332
BssMI GATC 7 cut(s) 75, 80, 109, 340, 645, 709, 1366
BssNI GRCGYC 1 cut(s) 689
BssT1I CCWWGG 4 cut(s) 276, 353, 873, 1332
Bst2UI CCWGG 2 cut(s) 966, 1318
Bst4CI ACNGT 3 cut(s) 149, 212, 685
Bst6I CTCTTC 2 cut(s) 240, 358
BstACI GRCGYC 1 cut(s) 689
BstDEI CTNAG 5 cut(s) 359, 432, 823, 1058, 1370
BstDSI CCRYGG 1 cut(s) 310
BstF5I GGATG 4 cut(s) 609, 692, 751, 1282
BstH2I RGCGCY 1 cut(s) 692
BstHHI GCGC 1 cut(s) 691
BstKTI GATC 7 cut(s) 78, 83, 112, 343, 648, 712, 1369
BstMAI GTCTC 3 cut(s) 572, 629, 1250
BstMBI GATC 7 cut(s) 75, 80, 109, 340, 645, 709, 1366
BstMWI GCNNNNNNNGC 3 cut(s) 56, 553, 947
BstNI CCWGG 2 cut(s) 966, 1318
BstNSI RCATGY 4 cut(s) 547, 1172, 1350, 1357
BstSCI CCNGG 4 cut(s) 62, 964, 1096, 1316
BstSFI CTRYAG 2 cut(s) 448, 713
BstSLI GKGCMC 1 cut(s) 1000
BstV1I GCAGC 3 cut(s) 68, 434, 937
BstV2I GAAGAC 1 cut(s) 1467
BstX2I RGATCY 2 cut(s) 709, 1366
BstXI CCANNNNNNTGG 4 cut(s) 46, 151, 699, 1386
BstYI RGATCY 2 cut(s) 709, 1366
BsuRI GGCC 2 cut(s) 38, 50
BtgI CCRYGG 1 cut(s) 310
BtrI CACGTC 1 cut(s) 1081
BtsCI GGATG 4 cut(s) 609, 692, 751, 1282
BtsI GCAGTG 1 cut(s) 624
BtsIMutI CAGTG 2 cut(s) 624, 1055
BveI ACCTGC 1 cut(s) 441
CciI TCATGA 1 cut(s) 834
CfoI GCGC 1 cut(s) 691
Cfr13I GGNCC 1 cut(s) 141
Csp6I GTAC 4 cut(s) 454, 1390, 1535, 1551
CviQI GTAC 4 cut(s) 454, 1390, 1535, 1551
DdeI CTNAG 5 cut(s) 359, 432, 823, 1058, 1370
DinI GGCGCC 1 cut(s) 690
DpnI GATC 7 cut(s) 77, 82, 111, 342, 647, 711, 1368
DpnII GATC 7 cut(s) 75, 80, 109, 340, 645, 709, 1366
DraIII CACNNNGTG 1 cut(s) 1273
DriI GACNNNNNGTC 1 cut(s) 147
EaeI YGGCCR 1 cut(s) 36
Eam1104I CTCTTC 2 cut(s) 240, 358
Eam1105I GACNNNNNGTC 1 cut(s) 147
EarI CTCTTC 2 cut(s) 240, 358
Eco130I CCWWGG 4 cut(s) 276, 353, 873, 1332
Eco47I GGWCC 1 cut(s) 141
Eco57I CTGAAG 2 cut(s) 264, 1479
EcoRII CCWGG 2 cut(s) 964, 1316
EcoT14I CCWWGG 4 cut(s) 276, 353, 873, 1332
EgeI GGCGCC 1 cut(s) 690
EheI GGCGCC 1 cut(s) 690
ErhI CCWWGG 4 cut(s) 276, 353, 873, 1332
Esp3I CGTCTC 1 cut(s) 629
FalI AAGNNNNNCTT 4 cut(s) 90, 122, 876, 908
FaqI GGGAC 5 cut(s) 498, 517, 694, 1113, 1422
FauNDI CATATG 1 cut(s) 403
FbaI TGATCA 1 cut(s) 109
Fnu4HI GCNGC 3 cut(s) 57, 448, 951
FokI GGATG 4 cut(s) 616, 679, 758, 1289
Fsp4HI GCNGC 3 cut(s) 57, 448, 951
FspBI CTAG 5 cut(s) 816, 1002, 1238, 1299, 1479
GlaI GCGC 1 cut(s) 690
GluI GCNGC 3 cut(s) 57, 448, 951
GsuI CTGGAG 1 cut(s) 578
HaeII RGCGCY 1 cut(s) 692
HaeIII GGCC 2 cut(s) 38, 50
HapII CCGG 3 cut(s) 35, 64, 1097
HhaI GCGC 1 cut(s) 691
Hin1I GRCGYC 1 cut(s) 689
Hin6I GCGC 1 cut(s) 689
HinP1I GCGC 1 cut(s) 689
HincII GTYRAC 1 cut(s) 12
HindII GTYRAC 1 cut(s) 12
HinfI GANTC 5 cut(s) 158, 422, 593, 889, 1374
HpaI GTTAAC 1 cut(s) 12
HpaII CCGG 3 cut(s) 35, 64, 1097
HphI GGTGA 6 cut(s) 107, 395, 890, 1259, 1348, 1464
Hpy166II GTNNAC 3 cut(s) 12, 240, 1339
Hpy188I TCNGA 9 cut(s) 80, 292, 433, 650, 697, 826, 1025, 1089, 1528
Hpy188III TCNNGA 4 cut(s) 107, 835, 1051, 1216
Hpy8I GTNNAC 3 cut(s) 12, 240, 1339
Hpy99I CGWCG 1 cut(s) 1207
HpyAV CCTTC 4 cut(s) 645, 832, 1109, 1479
HpyCH4III ACNGT 3 cut(s) 149, 212, 685
HpyCH4IV ACGT 1 cut(s) 1080
HpyCH4V TGCA 8 cut(s) 392, 450, 506, 617, 715, 953, 1123, 1522
HpyF10VI GCNNNNNNNGC 3 cut(s) 56, 553, 947
HpyF3I CTNAG 5 cut(s) 359, 432, 823, 1058, 1370
HpySE526I ACGT 1 cut(s) 1080
Hsp92I GRCGYC 1 cut(s) 689
HspAI GCGC 1 cut(s) 689
KasI GGCGCC 1 cut(s) 688
KpnI GGTACC 1 cut(s) 1393
Ksp22I TGATCA 1 cut(s) 109
KspAI GTTAAC 1 cut(s) 12
Kzo9I GATC 7 cut(s) 75, 80, 109, 340, 645, 709, 1366
LmnI GCTCC 3 cut(s) 444, 585, 1175
Lsp1109I GCAGC 3 cut(s) 68, 434, 937
LweI GCATC 3 cut(s) 397, 736, 1509
MaeI CTAG 5 cut(s) 816, 1002, 1238, 1299, 1479
MaeII ACGT 1 cut(s) 1080
MaeIII GTNAC 2 cut(s) 564, 1349
MalI GATC 7 cut(s) 77, 82, 111, 342, 647, 711, 1368
MboI GATC 7 cut(s) 75, 80, 109, 340, 645, 709, 1366
MboII GAAGA 7 cut(s) 173, 257, 375, 893, 1145, 1256, 1472
MfeI CAATTG 2 cut(s) 226, 1394
MflI RGATCY 2 cut(s) 709, 1366
MhlI GDGCHC 1 cut(s) 1000
Mly113I GGCGCC 1 cut(s) 689
MlyI GAGTC 2 cut(s) 416, 898
MmeI TCCRAC 2 cut(s) 705, 1153
MroXI GAANNNNTTC 2 cut(s) 102, 1464
MseI TTAA 2 cut(s) 11, 1440
MslI CAYNNNNRTG 4 cut(s) 44, 697, 1153, 1358
MspI CCGG 3 cut(s) 35, 64, 1097
MspR9I CCNGG 4 cut(s) 64, 966, 1098, 1318
MunI CAATTG 2 cut(s) 226, 1394
Mva1269I GAATGC 1 cut(s) 253
MvaI CCWGG 2 cut(s) 966, 1318
MwoI GCNNNNNNNGC 3 cut(s) 56, 553, 947
NarI GGCGCC 1 cut(s) 689
NciI CCSGG 2 cut(s) 64, 1098
NdeI CATATG 1 cut(s) 403
NdeII GATC 7 cut(s) 75, 80, 109, 340, 645, 709, 1366
NlaIV GGNNCC 5 cut(s) 690, 729, 999, 1086, 1391
NmuCI GTSAC 2 cut(s) 564, 1349
NspI RCATGY 4 cut(s) 547, 1172, 1350, 1357
OliI CACNNNNGTG 1 cut(s) 44
PagI TCATGA 1 cut(s) 834
PciI ACATGT 2 cut(s) 1168, 1353
PctI GAATGC 1 cut(s) 253
PdmI GAANNNNTTC 2 cut(s) 102, 1464
PfeI GAWTC 3 cut(s) 158, 593, 1374
PkrI GCNGC 3 cut(s) 58, 449, 952
PleI GAGTC 2 cut(s) 416, 897
PluTI GGCGCC 1 cut(s) 692
PpsI GAGTC 2 cut(s) 416, 897
PscI ACATGT 2 cut(s) 1168, 1353
PsiI TTATAA 1 cut(s) 867
Psp6I CCWGG 2 cut(s) 964, 1316
PspGI CCWGG 2 cut(s) 964, 1316
PspN4I GGNNCC 5 cut(s) 690, 729, 999, 1086, 1391
PspPI GGNCC 1 cut(s) 141
PstI CTGCAG 2 cut(s) 452, 717
PsuI RGATCY 2 cut(s) 709, 1366
RsaI GTAC 4 cut(s) 455, 1391, 1536, 1552
RsaNI GTAC 4 cut(s) 454, 1390, 1535, 1551
RseI CAYNNNNRTG 4 cut(s) 44, 697, 1153, 1358
SaqAI TTAA 2 cut(s) 11, 1440
SatI GCNGC 3 cut(s) 57, 448, 951
Sau3AI GATC 7 cut(s) 75, 80, 109, 340, 645, 709, 1366
Sau96I GGNCC 1 cut(s) 141
SchI GAGTC 2 cut(s) 416, 898
ScrFI CCNGG 4 cut(s) 64, 966, 1098, 1318
SduI GDGCHC 1 cut(s) 1000
SfaNI GCATC 3 cut(s) 397, 736, 1509
SfcI CTRYAG 2 cut(s) 448, 713
SfoI GGCGCC 1 cut(s) 690
SinI GGWCC 1 cut(s) 141
SmiMI CAYNNNNRTG 4 cut(s) 44, 697, 1153, 1358
SmlI CTYRAG 3 cut(s) 42, 1214, 1253
SmoI CTYRAG 3 cut(s) 42, 1214, 1253
SsiI CCGC 2 cut(s) 54, 665
SspDI GGCGCC 1 cut(s) 688
SspMI CTAG 5 cut(s) 816, 1002, 1238, 1299, 1479
StyD4I CCNGG 4 cut(s) 62, 964, 1096, 1316
StyI CCWWGG 4 cut(s) 276, 353, 873, 1332
TaaI ACNGT 3 cut(s) 149, 212, 685
TaiI ACGT 1 cut(s) 1083
TaqI TCGA 2 cut(s) 189, 414
TatI WGTACW 2 cut(s) 1534, 1550
TfiI GAWTC 3 cut(s) 158, 593, 1374
Tru1I TTAA 2 cut(s) 11, 1440
Tru9I TTAA 2 cut(s) 11, 1440
TscAI CASTG 2 cut(s) 624, 1062
TseFI GTSAC 2 cut(s) 564, 1349
TseI GCWGC 3 cut(s) 56, 447, 950
Tsp45I GTSAC 2 cut(s) 564, 1349
TspDTI ATGAA 6 cut(s) 851, 960, 978, 1128, 1235, 1577
TspRI CASTG 2 cut(s) 624, 1062
VpaK11BI GGWCC 1 cut(s) 141
XapI RAATTY 2 cut(s) 386, 1571
XceI RCATGY 4 cut(s) 547, 1172, 1350, 1357
XcmI CCANNNNNNNNNTGG 1 cut(s) 351
XmnI GAANNNNTTC 2 cut(s) 102, 1464
XspI CTAG 5 cut(s) 816, 1002, 1238, 1299, 1479
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.