Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb3

Orthogroup ID

OG0008964

Physical Location & Seq

Genomic Coordinates Reverse (-)

3293951 .. 3298619

Gene Structure 4669 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_3g05650.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2355 bp

ATGTCAGTTTCCGGCACAAAAAACTTCTTTAGGAGGTTTTGTATCGATGAATACCAAATAGATAGTGTTGCTCAGAGCAGCTTCTTCTCTAGTGATCTATTGCCATCTCTTGGAGCCAGAATCAACCAATCAACCAAGCTCCGAAAATACATTATATCGCCTTACAATCCTCGTTATAGGGCTTGGGAGATGTTACTCATTGTTCTCGTCATTTACTCTGCCTGGATTTGCCCATTCGAGTTTGCTTTCTTGACGTACAAGCAGGATGCTCTTTTTATCATCGACAACATTGTCAACGGTTTCTTTGCCATTGACATCTTCCTCACTTTCTTTGTTGCATATCTCGACAACCAGTCTTATCTTCTTGTCGACAATCCCAAACAAATTGCAATGAGATACATATCAACCTGGTTTATTTTCGATGTGTGTTCCACTGCTCCATTCCAGCCTATTAGCCTCATGTTCACAAATCATGGCAGCGAACTTGGCTTTAAAGTACTGAACATGCTCCGGCTCTGGCGCCTCAGACGAGTGAGCTCCCTTTTTGCAAGACTTGAGAAGGACATCCGATTCAATTACTTCTGGACTCGGTGCACAAAGCTCATTTCTGTGACCCTTTTTGCAGTGCATTGTGCCGGATGCTTTTACTATCTGATAGCAGACAGATACCCTGATTCAAAACAAACATGGATCGGTGCAGTGTACCCAAATTTCAAAGAAGATAGTCTTTGGAACAGATATGTCACTGCAATGTACTGGTCTATCACAACACTCACAACCACTGGCTATGGAGACTTGCATGCTGAGAACCCAAGGGAGATGCTGTTTGATATTTTCTACATGCTGTTCAACTTGGGATTGACAGCTTACCTCATCGGAAACATGACAAACCTTGTGGTTCATTCGACCAGCAGAACCAGAAACTTTAGGGACACGGTGCGAGCTGCTTCAGAATTTGCATCAAGAAATGACTTGCCACCAAGAATACATGACCAAATGTTGTCACACATATGCCTCAAATTCAGGACAGAAGGGTTGAAGCAGCAAGAGACCTTGAATGATCTCCCGAAAGCGCTTCGTTCAAGCATTGCTCAACATCTCTTCTTCCCCATTATTCAAAAAGTCGATATCTTCCGAGGAGTTTCATATGACTTCCTTTTCCAATTGGTATCAGAAATAGATGCCGAGTACTTTCCACCTAAAGAAGAAGTCATTCTGCAAAATGAGGCCCCGACGGATCTTTACATACTGGTCTCAGGTGCAGTAGTTTTGATATCCAATATTGATGGGCAAGAGCATATTGTAGGAAAGGCAAATGCTGGAGATACTTTGGGAGAAATTGGAGTATTATGTCACAGGCCACAGCCTTACACAGTTAGGACAACTGAACTTTCCCAAATATTACGACTCCGCAGAGATTCATTGATGACCACTATACAAACAAATAAGCAAGACGAACAGATTATCATGAACAACATTTTTATGAAACTGAAAGGCGAAGATAGCTCAGGCTTTGAATATCCGCACACAAATTCAGGCTTAATGCTCGACAATGATCGTGGAGGAGGACACAGAGGAGGATGCAAAGATAGCTCACATGAAGATGCAGCAATGCAGGAATCAAGGAACAATTGTTTTACAGCCTCGCAGGCTAGGAAGAAGAGTGAGATAGGCAAAGCTAAAATGACAGGTGAGAATGGCCAAACTGAACTTCATGCCGCCGTTGGCCAGGACCATCTGGAAAAGGTAAAAATTGTAGTTGAAGGAGGAGCAAACGTCAACAAACCGGAACCTAGAGGATGGGTGCCAAAAGGTCCAGCACAACAGCGAGGAGACAATAGCATGCATGACCTCTCATTAAGTTATGAAAATAGGACAGAAATAGATGACCATAGAATAGAGTTTATTGAGCCAGAAACATCTGGAAGCACCAGCAATTGTGAAGGAAATTGCAGACGACAGGAAGACCACCAACATATTCATTCTCACTTAAGAGAGGTATCCATGAAGTCCTATCCATGCCCGTCTAGCCCTGCAACTGACAAAGAAGATGGGATCAGATCATACAGCAAGAGAGTAACTATCCACATGCATTCTAAAAGTGAAAATGGACCGGAAAGGCAACTTGCAAAGTTAATAATTCTACCTGATTCCATAGACCAGCTGCTCAGAGTTGCCGACTGTGACATTGCAGGTGAGAAGTTTGGAGGCTACAGACCTACGAAAGTCGTGAATGCAGAGAATGCAGAAATAGAAGACATCAATGTTGTTAGAGATGGTGATCATCTGTTTCTTCTTCATGATGATTGTCAAAGTGTGAACTCTGATGTGACTTCATATCGTTCAAAGATGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005216 GO:0005242 GO:0005244 GO:0005249 GO:0005261 GO:0005267 GO:0005488 GO:0005515 GO:0005575 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0007623 GO:0008150 GO:0008324 GO:0009314 GO:0009416 GO:0009628 GO:0009642 GO:0009644 GO:0009987 GO:0010118 GO:0015075 GO:0015077 GO:0015079 GO:0015267 GO:0015276 GO:0015318 GO:0015672 GO:0016020 GO:0016021 GO:0022803 GO:0022832 GO:0022834 GO:0022836 GO:0022838 GO:0022839 GO:0022843 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042391 GO:0042802 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0048511 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0065007 GO:0065008 GO:0071804 GO:0071805 GO:0071944 GO:0098655 GO:0098660 GO:0098662 GO:0099094

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ank_2 Ion_trans KHA cNMP_binding

Protein Analysis

785

Amino Acids

89.55

Weight (kDa)

6.2

Isoelectric Point (pI)

46.29

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ion_trans	PF00520	59 - 306	2.9e-44	Ion transport protein
Ion_trans_2	PF07885	219 - 299	1.2e-12	Ion channel
cNMP_binding	PF00027	397 - 482	1.4e-16	Cyclic nucleotide-binding domain
KHA	PF11834	691 - 760	5.4e-25	KHA, dimerisation domain of potassium ion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008964)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G18290 AT4G18290 AT5G46240
fragaria_vesca	FvH4_3g05650 FvH4_3g05650
malus_domestica	MD05G1284400.v1.1 MD10G1262800.v1.1
prunus_persica	Prupe.4G080000_v2.0.a1 Prupe.4G080000_v2.0.a1
pyrus_communis	pycom05g26360
rosa_chinensis	RchiOBHm_Chr5g0013991
rosa_laevigata	RLG00000032060
rosa_multiflora	Rmu_co8232297.1_g000001 Rmu_co8474133.1_g000001 Rmu_sc0005803.1_g000012
rosa_roxburghii	Rroxscaffold_1G00062720
rosa_rugosa	Rorug05G0013900
rosa_samantha	Rh5AG108400 Rh5BG105200 Rh5CG117000 Rh5DG103900
rosa_wichuraiana	Rw5G009460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2183
AasI	GACNNNNNNGTC	1 cut(s)	290
Acc36I	ACCTGC	1 cut(s)	2183
AccB1I	GGYRCC	2 cut(s)	519, 1804
AccB7I	CCANNNNNTGG	1 cut(s)	110
AccI	GTMKAC	1 cut(s)	369
AciI	CCGC	3 cut(s)	1411, 1523, 1719
AclWI	GGATC	3 cut(s)	698, 1245, 2063
AcoI	YGGCCR	2 cut(s)	1699, 1726
AcsI	RAATTY	4 cut(s)	709, 953, 1019, 1531
AcuI	CTGAAG	1 cut(s)	933
AcyI	GRCGYC	1 cut(s)	520
AfaI	GTAC	5 cut(s)	257, 498, 704, 755, 1190
AfeI	AGCGCT	1 cut(s)	1074
AfiI	CCNNNNNNNGG	1 cut(s)	110
AflII	CTTAAG	1 cut(s)	1990
AhdI	GACNNNNNGTC	1 cut(s)	352
AjnI	CCWGG	3 cut(s)	221, 407, 1728
AjuI	GAANNNNNNNTTGG	2 cut(s)	1695, 1727
Alw21I	GWGCWC	2 cut(s)	539, 596
Alw26I	GTCTC	4 cut(s)	786, 1043, 1258, 1827
Alw44I	GTGCAC	1 cut(s)	592
AlwI	GGATC	3 cut(s)	698, 1245, 2063
Aor51HI	AGCGCT	1 cut(s)	1074
AoxI	GGCC	4 cut(s)	1227, 1358, 1699, 1726
ApaLI	GTGCAC	1 cut(s)	592
ApeKI	GCWGC	6 cut(s)	78, 477, 944, 1042, 1607, 2164
ApoI	RAATTY	4 cut(s)	709, 953, 1019, 1531
Asp700I	GAANNNNTTC	1 cut(s)	1212
AspLEI	GCGC	2 cut(s)	522, 1075
AspS9I	GGNCC	4 cut(s)	1228, 1732, 1814, 2111
AsuHPI	GGTGA	3 cut(s)	1703, 2207, 2291
AvaII	GGWCC	3 cut(s)	1732, 1814, 2111
BaeGI	GKGCMC	1 cut(s)	596
BalI	TGGCCA	2 cut(s)	1701, 1728
BanI	GGYRCC	2 cut(s)	519, 1804
BanII	GRGCYC	1 cut(s)	539
BbsI	GAAGAC	2 cut(s)	1971, 2262
Bbv12I	GWGCWC	2 cut(s)	539, 596
BbvI	GCAGC	6 cut(s)	90, 489, 931, 1054, 1619, 2151
BccI	CCATC	6 cut(s)	112, 1280, 1743, 1794, 2045, 2270
BceAI	ACGGC	1 cut(s)	1706
BciT130I	CCWGG	3 cut(s)	223, 409, 1730
BciVI	GTATCC	1 cut(s)	2011
BclI	TGATCA	1 cut(s)	2281
BcoDI	GTCTC	4 cut(s)	786, 1043, 1258, 1827
BfaI	CTAG	4 cut(s)	90, 1653, 1794, 2028
BfmI	CTRYAG	1 cut(s)	2212
BfoI	RGCGCY	2 cut(s)	523, 1076
BfrI	CTTAAG	1 cut(s)	1990
BfuAI	ACCTGC	1 cut(s)	2183
BfuI	GTATCC	1 cut(s)	2011
BglI	GCCNNNNNGGC	1 cut(s)	1649
BisI	GCNGC	7 cut(s)	79, 478, 945, 1043, 1608, 1719, 2165
BlsI	GCNGC	7 cut(s)	80, 479, 946, 1044, 1609, 1720, 2166
BmcAI	AGTACT	2 cut(s)	498, 1190
Bme1390I	CCNGG	3 cut(s)	223, 409, 1730
Bme18I	GGWCC	3 cut(s)	1732, 1814, 2111
BmeRI	GACNNNNNGTC	1 cut(s)	352
BmgT120I	GGNCC	4 cut(s)	1228, 1732, 1814, 2111
BmiI	GGNNCC	5 cut(s)	115, 521, 1230, 1791, 1806
BmrFI	CCNGG	3 cut(s)	223, 409, 1730
BmsI	GCATC	7 cut(s)	256, 629, 810, 968, 1171, 1571, 1594
BpiI	GAAGAC	2 cut(s)	1971, 2262
BpmI	CTGGAG	1 cut(s)	1341
Bpu10I	CCTNAGC	1 cut(s)	1507
BpuEI	CTTGAG	1 cut(s)	575
Bsa29I	ATCGAT	1 cut(s)	45
BsaBI	GATNNNNATC	2 cut(s)	400, 2280
BsaHI	GRCGYC	1 cut(s)	520
BsaI	GGTCTC	2 cut(s)	1043, 1258
BsaJI	CCNNGG	2 cut(s)	812, 1135
BsaWI	WCCGGW	2 cut(s)	1786, 2113
BsaXI	ACNNNNNCTCC	6 cut(s)	25, 55, 809, 839, 1761, 1791
Bsc4I	CCNNNNNNNGG	1 cut(s)	110
Bse1I	ACTGG	4 cut(s)	352, 761, 787, 1254
Bse3DI	GCAATG	5 cut(s)	396, 756, 1086, 1617, 2187
Bse8I	GATNNNNATC	2 cut(s)	400, 2280
BseBI	CCWGG	3 cut(s)	223, 409, 1730
BseCI	ATCGAT	1 cut(s)	45
BseDI	CCNNGG	2 cut(s)	812, 1135
BseGI	GGATG	5 cut(s)	271, 564, 644, 1586, 1805
BseJI	GATNNNNATC	2 cut(s)	400, 2280
BseLI	CCNNNNNNNGG	1 cut(s)	110
BseMI	GCAATG	5 cut(s)	396, 756, 1086, 1617, 2187
BseMII	CTCAG	6 cut(s)	86, 538, 795, 1269, 1521, 2182
BseNI	ACTGG	4 cut(s)	352, 761, 787, 1254
BseRI	GAGGAG	5 cut(s)	1152, 1578, 1590, 1782, 1845
BseSI	GKGCMC	1 cut(s)	596
BseXI	GCAGC	6 cut(s)	90, 489, 931, 1054, 1619, 2151
BsgI	GTGCAG	2 cut(s)	717, 1281
BshFI	GGCC	4 cut(s)	1229, 1360, 1701, 1728
BshNI	GGYRCC	2 cut(s)	519, 1804
BshVI	ATCGAT	1 cut(s)	45
BsiHKAI	GWGCWC	2 cut(s)	539, 596
BsiSI	CCGG	5 cut(s)	12, 511, 636, 1787, 2114
BslFI	GGGAC	1 cut(s)	944
BslI	CCNNNNNNNGG	1 cut(s)	110
BsmAI	GTCTC	4 cut(s)	786, 1043, 1258, 1827
BsmFI	GGGAC	1 cut(s)	944
BsmI	GAATGC	3 cut(s)	2092, 2239, 2248
BsnI	GGCC	4 cut(s)	1229, 1360, 1701, 1728
Bso31I	GGTCTC	2 cut(s)	1043, 1258
Bsp1286I	GDGCHC	2 cut(s)	539, 596
Bsp143I	GATC	8 cut(s)	94, 690, 1060, 1237, 1555, 2055, 2060, 2281
BspACI	CCGC	3 cut(s)	1411, 1523, 1719
BspANI	GGCC	4 cut(s)	1229, 1360, 1701, 1728
BspCNI	CTCAG	6 cut(s)	85, 537, 796, 1268, 1520, 2181
BspDI	ATCGAT	1 cut(s)	45
BspHI	TCATGA	2 cut(s)	1467, 2299
BspLI	GGNNCC	5 cut(s)	115, 521, 1230, 1791, 1806
BspMI	ACCTGC	1 cut(s)	2183
BspPI	GGATC	3 cut(s)	698, 1245, 2063
BspT107I	GGYRCC	2 cut(s)	519, 1804
BspTI	CTTAAG	1 cut(s)	1990
BspTNI	GGTCTC	2 cut(s)	1043, 1258
BsrDI	GCAATG	5 cut(s)	396, 756, 1086, 1617, 2187
BsrI	ACTGG	4 cut(s)	352, 761, 787, 1254
BssECI	CCNNGG	2 cut(s)	812, 1135
BssMI	GATC	8 cut(s)	94, 690, 1060, 1237, 1555, 2055, 2060, 2281
BssNI	GRCGYC	1 cut(s)	520
BssT1I	CCWWGG	1 cut(s)	812
Bst2UI	CCWGG	3 cut(s)	223, 409, 1730
Bst4CI	ACNGT	4 cut(s)	299, 937, 1375, 2183
Bst6I	CTCTTC	2 cut(s)	1106, 1655
BstACI	GRCGYC	1 cut(s)	520
BstAFI	CTTAAG	1 cut(s)	1990
BstAPI	GCANNNNNTGC	1 cut(s)	2243
BstC8I	GCNNGC	4 cut(s)	801, 942, 1650, 1844
BstDEI	CTNAG	6 cut(s)	72, 524, 804, 1255, 1507, 2168
BstF5I	GGATG	5 cut(s)	271, 564, 644, 1586, 1805
BstH2I	RGCGCY	2 cut(s)	523, 1076
BstHHI	GCGC	2 cut(s)	522, 1075
BstKTI	GATC	8 cut(s)	97, 693, 1063, 1240, 1558, 2058, 2063, 2284
BstMAI	GTCTC	4 cut(s)	786, 1043, 1258, 1827
BstMBI	GATC	8 cut(s)	94, 690, 1060, 1237, 1555, 2055, 2060, 2281
BstMWI	GCNNNNNNNGC	6 cut(s)	486, 1503, 1590, 1649, 2028, 2243
BstNI	CCWGG	3 cut(s)	223, 409, 1730
BstNSI	RCATGY	5 cut(s)	508, 803, 844, 1846, 2092
BstSCI	CCNGG	3 cut(s)	221, 407, 1728
BstSFI	CTRYAG	1 cut(s)	2212
BstSLI	GKGCMC	1 cut(s)	596
BstV1I	GCAGC	6 cut(s)	90, 489, 931, 1054, 1619, 2151
BstV2I	GAAGAC	2 cut(s)	1971, 2262
BstX2I	RGATCY	1 cut(s)	1237
BstYI	RGATCY	1 cut(s)	1237
Bsu15I	ATCGAT	1 cut(s)	45
BsuI	GTATCC	1 cut(s)	2011
BsuRI	GGCC	4 cut(s)	1229, 1360, 1701, 1728
BsuTUI	ATCGAT	1 cut(s)	45
BtsCI	GGATG	5 cut(s)	271, 564, 644, 1586, 1805
BtsI	GCAGTG	4 cut(s)	432, 630, 705, 744
BtsIMutI	CAGTG	5 cut(s)	432, 630, 705, 744, 780
BveI	ACCTGC	1 cut(s)	2183
Cac8I	GCNNGC	4 cut(s)	801, 942, 1650, 1844
CciI	TCATGA	2 cut(s)	1467, 2299
CfoI	GCGC	2 cut(s)	522, 1075
Cfr13I	GGNCC	4 cut(s)	1228, 1732, 1814, 2111
ClaI	ATCGAT	1 cut(s)	45
CsiI	ACCWGGT	1 cut(s)	407
Csp6I	GTAC	5 cut(s)	256, 497, 703, 754, 1189
CspCI	CAANNNNNGTGG	4 cut(s)	876, 911, 1540, 1575
CviQI	GTAC	5 cut(s)	256, 497, 703, 754, 1189
DdeI	CTNAG	6 cut(s)	72, 524, 804, 1255, 1507, 2168
DinI	GGCGCC	1 cut(s)	521
DpnI	GATC	8 cut(s)	96, 692, 1062, 1239, 1557, 2057, 2062, 2283
DpnII	GATC	8 cut(s)	94, 690, 1060, 1237, 1555, 2055, 2060, 2281
DraI	TTTAAA	1 cut(s)	493
DrdI	GACNNNNNNGTC	1 cut(s)	290
DriI	GACNNNNNGTC	1 cut(s)	352
DseDI	GACNNNNNNGTC	1 cut(s)	290
EaeI	YGGCCR	2 cut(s)	1699, 1726
Eam1104I	CTCTTC	2 cut(s)	1106, 1655
Eam1105I	GACNNNNNGTC	1 cut(s)	352
EarI	CTCTTC	2 cut(s)	1106, 1655
Ecl136II	GAGCTC	1 cut(s)	537
Eco130I	CCWWGG	1 cut(s)	812
Eco24I	GRGCYC	1 cut(s)	539
Eco31I	GGTCTC	2 cut(s)	1043, 1258
Eco32I	GATATC	2 cut(s)	1129, 1275
Eco47I	GGWCC	3 cut(s)	1732, 1814, 2111
Eco47III	AGCGCT	1 cut(s)	1074
Eco53kI	GAGCTC	1 cut(s)	537
Eco57I	CTGAAG	1 cut(s)	933
EcoICRI	GAGCTC	1 cut(s)	537
EcoO109I	RGGNCCY	1 cut(s)	1228
EcoRII	CCWGG	3 cut(s)	221, 407, 1728
EcoRV	GATATC	2 cut(s)	1129, 1275
EcoT14I	CCWWGG	1 cut(s)	812
EcoT22I	ATGCAT	2 cut(s)	1848, 2094
EcoT38I	GRGCYC	1 cut(s)	539
EgeI	GGCGCC	1 cut(s)	521
EheI	GGCGCC	1 cut(s)	521
ErhI	CCWWGG	1 cut(s)	812
FalI	AAGNNNNNCTT	2 cut(s)	711, 743
FaqI	GGGAC	1 cut(s)	944
FauNDI	CATATG	2 cut(s)	1010, 1147
FbaI	TGATCA	1 cut(s)	2281
FblI	GTMKAC	1 cut(s)	369
Fnu4HI	GCNGC	7 cut(s)	79, 478, 945, 1043, 1608, 1719, 2165
FokI	GGATG	5 cut(s)	278, 551, 651, 1593, 1812
FriOI	GRGCYC	1 cut(s)	539
Fsp4HI	GCNGC	7 cut(s)	79, 478, 945, 1043, 1608, 1719, 2165
FspBI	CTAG	4 cut(s)	90, 1653, 1794, 2028
GlaI	GCGC	2 cut(s)	521, 1074
GluI	GCNGC	7 cut(s)	79, 478, 945, 1043, 1608, 1719, 2165
GsuI	CTGGAG	1 cut(s)	1341
HaeII	RGCGCY	2 cut(s)	523, 1076
HaeIII	GGCC	4 cut(s)	1229, 1360, 1701, 1728
HapII	CCGG	5 cut(s)	12, 511, 636, 1787, 2114
HhaI	GCGC	2 cut(s)	522, 1075
Hin1I	GRCGYC	1 cut(s)	520
Hin6I	GCGC	2 cut(s)	520, 1073
HinP1I	GCGC	2 cut(s)	520, 1073
HincII	GTYRAC	3 cut(s)	295, 370, 1780
HindII	GTYRAC	3 cut(s)	295, 370, 1780
HinfI	GANTC	8 cut(s)	120, 570, 586, 674, 1407, 1418, 1619, 2150
HpaII	CCGG	5 cut(s)	12, 511, 636, 1787, 2114
HphI	GGTGA	3 cut(s)	1703, 2207, 2291
Hpy166II	GTNNAC	7 cut(s)	295, 370, 465, 594, 703, 1780, 2320
Hpy8I	GTNNAC	7 cut(s)	295, 370, 465, 594, 703, 1780, 2320
Hpy99I	CGWCG	1 cut(s)	1237
HpyAV	CCTTC	4 cut(s)	553, 1025, 1757, 1937
HpyCH4III	ACNGT	4 cut(s)	299, 937, 1375, 2183
HpyCH4IV	ACGT	2 cut(s)	254, 1776
HpyF10VI	GCNNNNNNNGC	6 cut(s)	486, 1503, 1590, 1649, 2028, 2243
HpyF3I	CTNAG	6 cut(s)	72, 524, 804, 1255, 1507, 2168
HpySE526I	ACGT	2 cut(s)	254, 1776
Hsp92I	GRCGYC	1 cut(s)	520
HspAI	GCGC	2 cut(s)	520, 1073
KasI	GGCGCC	1 cut(s)	519
Ksp22I	TGATCA	1 cut(s)	2281
Kzo9I	GATC	8 cut(s)	94, 690, 1060, 1237, 1555, 2055, 2060, 2281
LmnI	GCTCC	6 cut(s)	113, 144, 442, 513, 542, 1769
Lsp1109I	GCAGC	6 cut(s)	90, 489, 931, 1054, 1619, 2151
LweI	GCATC	7 cut(s)	256, 629, 810, 968, 1171, 1571, 1594
MabI	ACCWGGT	1 cut(s)	407
MaeI	CTAG	4 cut(s)	90, 1653, 1794, 2028
MaeII	ACGT	2 cut(s)	254, 1776
MaeIII	GTNAC	8 cut(s)	192, 610, 742, 1002, 1352, 2077, 2183, 2329
MalI	GATC	8 cut(s)	96, 692, 1062, 1239, 1557, 2057, 2062, 2283
MboI	GATC	8 cut(s)	94, 690, 1060, 1237, 1555, 2055, 2060, 2281
MfeI	CAATTG	3 cut(s)	1163, 1630, 1936
MflI	RGATCY	1 cut(s)	1237
MhlI	GDGCHC	2 cut(s)	539, 596
MlsI	TGGCCA	2 cut(s)	1701, 1728
MluNI	TGGCCA	2 cut(s)	1701, 1728
Mly113I	GGCGCC	1 cut(s)	520
MlyI	GAGTC	2 cut(s)	580, 1401
Mox20I	TGGCCA	2 cut(s)	1701, 1728
Mph1103I	ATGCAT	2 cut(s)	1848, 2094
MroXI	GAANNNNTTC	1 cut(s)	1212
MscI	TGGCCA	2 cut(s)	1701, 1728
MseI	TTAA	5 cut(s)	492, 1541, 1859, 1991, 2135
MslI	CAYNNNNRTG	5 cut(s)	608, 749, 1009, 1481, 1602
Msp20I	TGGCCA	2 cut(s)	1701, 1728
MspA1I	CMGCKG	1 cut(s)	2164
MspCI	CTTAAG	1 cut(s)	1990
MspI	CCGG	5 cut(s)	12, 511, 636, 1787, 2114
MspR9I	CCNGG	3 cut(s)	223, 409, 1730
MunI	CAATTG	3 cut(s)	1163, 1630, 1936
Mva1269I	GAATGC	3 cut(s)	2092, 2239, 2248
MvaI	CCWGG	3 cut(s)	223, 409, 1730
MwoI	GCNNNNNNNGC	6 cut(s)	486, 1503, 1590, 1649, 2028, 2243
NarI	GGCGCC	1 cut(s)	520
NdeI	CATATG	2 cut(s)	1010, 1147
NdeII	GATC	8 cut(s)	94, 690, 1060, 1237, 1555, 2055, 2060, 2281
NlaIV	GGNNCC	5 cut(s)	115, 521, 1230, 1791, 1806
NmeAIII	GCCGAG	1 cut(s)	1210
NmuCI	GTSAC	6 cut(s)	610, 742, 1002, 1352, 2183, 2329
NsiI	ATGCAT	2 cut(s)	1848, 2094
NspI	RCATGY	5 cut(s)	508, 803, 844, 1846, 2092
PaeI	GCATGC	2 cut(s)	803, 1846
PagI	TCATGA	2 cut(s)	1467, 2299
PaqCI	CACCTGC	1 cut(s)	2183
PctI	GAATGC	3 cut(s)	2092, 2239, 2248
PdmI	GAANNNNTTC	1 cut(s)	1212
PfeI	GAWTC	6 cut(s)	120, 570, 674, 1418, 1619, 2150
PflMI	CCANNNNNTGG	1 cut(s)	110
PkrI	GCNGC	7 cut(s)	80, 479, 946, 1044, 1609, 1720, 2166
PleI	GAGTC	2 cut(s)	580, 1401
PluTI	GGCGCC	1 cut(s)	523
PpsI	GAGTC	2 cut(s)	580, 1401
Psp124BI	GAGCTC	1 cut(s)	539
Psp6I	CCWGG	3 cut(s)	221, 407, 1728
PspGI	CCWGG	3 cut(s)	221, 407, 1728
PspN4I	GGNNCC	5 cut(s)	115, 521, 1230, 1791, 1806
PspPI	GGNCC	4 cut(s)	1228, 1732, 1814, 2111
PsuI	RGATCY	1 cut(s)	1237
PvuII	CAGCTG	1 cut(s)	2164
RsaI	GTAC	5 cut(s)	257, 498, 704, 755, 1190
RsaNI	GTAC	5 cut(s)	256, 497, 703, 754, 1189
RseI	CAYNNNNRTG	5 cut(s)	608, 749, 1009, 1481, 1602
SacI	GAGCTC	1 cut(s)	539
SalI	GTCGAC	1 cut(s)	368
SaqAI	TTAA	5 cut(s)	492, 1541, 1859, 1991, 2135
SatI	GCNGC	7 cut(s)	79, 478, 945, 1043, 1608, 1719, 2165
Sau3AI	GATC	8 cut(s)	94, 690, 1060, 1237, 1555, 2055, 2060, 2281
Sau96I	GGNCC	4 cut(s)	1228, 1732, 1814, 2111
ScaI	AGTACT	2 cut(s)	498, 1190
SchI	GAGTC	2 cut(s)	580, 1401
ScrFI	CCNGG	3 cut(s)	223, 409, 1730
SduI	GDGCHC	2 cut(s)	539, 596
SexAI	ACCWGGT	1 cut(s)	407
SfaNI	GCATC	7 cut(s)	256, 629, 810, 968, 1171, 1571, 1594
SfcI	CTRYAG	1 cut(s)	2212
SfoI	GGCGCC	1 cut(s)	521
SinI	GGWCC	3 cut(s)	1732, 1814, 2111
SmiMI	CAYNNNNRTG	5 cut(s)	608, 749, 1009, 1481, 1602
SmlI	CTYRAG	2 cut(s)	554, 1990
SmoI	CTYRAG	2 cut(s)	554, 1990
SphI	GCATGC	2 cut(s)	803, 1846
SsiI	CCGC	3 cut(s)	1411, 1523, 1719
SspDI	GGCGCC	1 cut(s)	519
SspI	AATATT	2 cut(s)	1282, 1401
SspMI	CTAG	4 cut(s)	90, 1653, 1794, 2028
SstI	GAGCTC	1 cut(s)	539
StyD4I	CCNGG	3 cut(s)	221, 407, 1728
StyI	CCWWGG	1 cut(s)	812
TaaI	ACNGT	4 cut(s)	299, 937, 1375, 2183
TaiI	ACGT	2 cut(s)	257, 1779
TaqI	TCGA	9 cut(s)	45, 237, 282, 345, 369, 420, 905, 1125, 1548
TatI	WGTACW	3 cut(s)	496, 753, 1188
TauI	GCSGC	1 cut(s)	1721
TfiI	GAWTC	6 cut(s)	120, 570, 674, 1418, 1619, 2150
Tru1I	TTAA	5 cut(s)	492, 1541, 1859, 1991, 2135
Tru9I	TTAA	5 cut(s)	492, 1541, 1859, 1991, 2135
TscAI	CASTG	5 cut(s)	439, 630, 705, 751, 787
TseFI	GTSAC	6 cut(s)	610, 742, 1002, 1352, 2183, 2329
TseI	GCWGC	6 cut(s)	78, 477, 944, 1042, 1607, 2164
Tsp45I	GTSAC	6 cut(s)	610, 742, 1002, 1352, 2183, 2329
TspGWI	ACGGA	1 cut(s)	1250
TspRI	CASTG	5 cut(s)	439, 630, 705, 751, 787
Van91I	CCANNNNNTGG	1 cut(s)	110
Vha464I	CTTAAG	1 cut(s)	1990
VneI	GTGCAC	1 cut(s)	592
VpaK11BI	GGWCC	3 cut(s)	1732, 1814, 2111
XapI	RAATTY	4 cut(s)	709, 953, 1019, 1531
XceI	RCATGY	5 cut(s)	508, 803, 844, 1846, 2092
XmiI	GTMKAC	1 cut(s)	369
XmnI	GAANNNNTTC	1 cut(s)	1212
XspI	CTAG	4 cut(s)	90, 1653, 1794, 2028
ZrmI	AGTACT	2 cut(s)	498, 1190
Zsp2I	ATGCAT	2 cut(s)	1848, 2094

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.