Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr5

Orthogroup ID

OG0008964

Physical Location & Seq

Genomic Coordinates Forward (+)

10166726 .. 10170240

Gene Structure 3515 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw5G009460.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2307 bp

ATGTCAGTTTCCGGCACAAAGAATTTCTTCAGGAGGTTTTGTATTGATGAATACCAAATAGATAGTGTTGCTCAGAGCAGCTTCTTCTCTAGTGATCTATTGCCATCTCTTGGAGCCAGAATCAACCAATCAACCAAGCTCCGAAAATACATTATATCGCCTTACAATCCTCGTTATAGGGCTTGGGAGATGTTACTCATTGTTCTCGTCATTTACTCCGCCTGGATTTGCCCATTCGAGTTTGCATTCTTGACTTACAAGCAAGATGCTCTTTTTGTCATCGACAACATTGTCAACGGTTTCTTTGCCATTGACATCTTCCTCACTTTCTTTGTTGCATATCTCGACAGCCAGTCTTATCTTCTTGTTGACAATCCCAAGCAAATTGCAATGAGGTACATATCAACCTGGTTTATTTTCGATGTGTGTTCCACTGCCCCATTCCAGCCTATTATTAGCCTCTTGTTTACAAATCATGGCAGCAAACTTGGCTTTAAGGTACTGAACATGCTCCGGCTCTGGCGCCTCAGACGAGTGAGCTCCCTGTTTGCAAGACTTGAGAAGGACATTCGATTCAATTACTTCTGGACTCGGTGCACAAAGCTCATTTCTGTGACCCTTTTCGCAGTACATTGTGCTGGTTGCTTTTACTATCTCATAGCAGACAGATACCCCGATTCAAAACAAACATGGATCGGTGCAGTGTACCCAAATTTCAAAGAGGATAGTCTTTGGAACAGATATGTCACTGCAATGTACTGGTCTATCACAACACTCACAACCACTGGCTATGGAGACTTGCATGCTGAGAACCCAAGGGAGATGCTGTTCGATATTTTCTACATGCTGTTCAACTTGGGATTGACAGCTTACCTCATCGGAAACATGACAAACCTTGTGGTTCATTCGACCAGCAGAACCAGAATCTTTAGGGACACGGTGCGAGCTGCTTCAGAATTTGCAGCAAGAAATGACTTGCCGCCAAGAATACAGGACCAAATGTTGTCACACATATGCCTCAAGTTCAGGACAGAAGGGTTGAAGCAGCAAGAGACCTTGAATGATCTCCCCAAAGCACTTCGTTCAAGCATTGCTCAGCATCTCTTCTTCCCCATTATTCAAAAGGTCGATATCTTCCAAGGAGTTTCATATGACTTCCTTTTCCAATTGGTATCAGAAATAGATGCCGAGTACTTTCCACCCAAAGAAGAAGTCATTCTGCAAAATGAGGCCCCAACGGATCTTTACATACTGGTCTCAGGTGCAGTAGTTTTAATATCCAATATTGATGGGCAAGAGCAAGTTGTAGGAAAGGCAAATTCTGGGGATACTTTGGGCGAAATTGGAGTATTATGTCAGAGGCCACAGCCTTACACAGTTCGGACAACTGAACTTTCCCAAATATTACGACTCCGCAGAAATTCACTGATGACCACTATACAAACAAATAAGGAAGACGAGCAGATTATCATGAACAACATTTTTATGAAACTGAAAGGGGAAGATGGCTCAGGCTTTGAATATCCACATACAAATCCAGGCTTAATGCTTGACAATTGTCCTGGAGGAGGACAGAGAGGAGGATGCAAAGATAGCTCACATGAAGATGCAGCAATGCAGGAAGCAAGGAACAATTGCTTTACAGCTTCGCAGGCTAGGATGAAGAGTGAAATAGGCAAAGCTCAAATGACAGGTGAGAATGGCCAAACTGAAATTCAAGCTGCTGTTTGCCAGGGCCATCTGGAAAAGGTAAAAATTGTGGTTGAAGGAGGAGCAGATGTCAACAAACCGGAAGCTAGAGGATGGATGCCAAAAGGAGACAAGGACATACATGACCTCTTATTAAGTTATGAAAATAGAAGAGAAATGGATGAACATAGAATAGAGTTCATTGAGCCAGAAACATCTGGAAGCACCAGCAATTGTGAAGGAAATTGTAAAACACAGGAAGACCACCAACATATTCATTCTCACTTGAGAAAGGTAGCCATGAAGTCATATCAGTGCCCGTCTAGCCCTGCAAGTGACAGAGAACAAAGGATCAGATCATACAGAAAGAGAGTAACTATCCACATGCATTCTAAAAGTAAAAGTGTGTCGGAAAGGCAGCTTGCAAAATTAATAATTCTACCTGATTCCATAGACGAGCTGCTCAGAGTTGCTAGTGAGAAGTTTGGAGGCTATAGACCTACAAAAGTCGTGAATGCAGAAAATGCAGAAATAGAAGACATGAGTGTCGTTAGAGATGGTGATCATCTGTTTCTTCTTCATAATGATTGTCAAAATTTGTACTCTGATGTGACCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005216 GO:0005242 GO:0005244 GO:0005249 GO:0005261 GO:0005267 GO:0005488 GO:0005515 GO:0005575 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0007623 GO:0008150 GO:0008324 GO:0009314 GO:0009416 GO:0009628 GO:0009642 GO:0009644 GO:0009987 GO:0010118 GO:0015075 GO:0015077 GO:0015079 GO:0015267 GO:0015276 GO:0015318 GO:0015672 GO:0016020 GO:0016021 GO:0022803 GO:0022832 GO:0022834 GO:0022836 GO:0022838 GO:0022839 GO:0022843 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042391 GO:0042802 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0048511 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0065007 GO:0065008 GO:0071804 GO:0071805 GO:0071944 GO:0098655 GO:0098660 GO:0098662 GO:0099094

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ank_2 Ion_trans KHA cNMP_binding

Protein Analysis

768

Amino Acids

87.87

Weight (kDa)

6.46

Isoelectric Point (pI)

42.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ion_trans	PF00520	59 - 307	3.5e-44	Ion transport protein
Ion_trans_2	PF07885	220 - 300	1.1e-12	Ion channel
cNMP_binding	PF00027	398 - 483	2.5e-16	Cyclic nucleotide-binding domain
KHA	PF11834	686 - 755	2.3e-24	KHA, dimerisation domain of potassium ion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008964)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G18290 AT4G18290 AT5G46240
fragaria_vesca	FvH4_3g05650 FvH4_3g05650
malus_domestica	MD05G1284400.v1.1 MD10G1262800.v1.1
prunus_persica	Prupe.4G080000_v2.0.a1 Prupe.4G080000_v2.0.a1
pyrus_communis	pycom05g26360
rosa_chinensis	RchiOBHm_Chr5g0013991
rosa_laevigata	RLG00000032060
rosa_multiflora	Rmu_co8232297.1_g000001 Rmu_co8474133.1_g000001 Rmu_sc0005803.1_g000012
rosa_roxburghii	Rroxscaffold_1G00062720
rosa_rugosa	Rorug05G0013900
rosa_samantha	Rh5AG108400 Rh5BG105200 Rh5CG117000 Rh5DG103900
rosa_wichuraiana	Rw5G009460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	290, 2234
AccB1I	GGYRCC	1 cut(s)	522
AccB7I	CCANNNNNTGG	1 cut(s)	110
AciI	CCGC	3 cut(s)	219, 980, 1414
AclWI	GGATC	3 cut(s)	701, 1248, 2048
AcoI	YGGCCR	1 cut(s)	1702
AcsI	RAATTY	7 cut(s)	22, 712, 956, 1318, 1420, 1713, 2284
AcuI	CTGAAG	2 cut(s)	13, 936
AcyI	GRCGYC	1 cut(s)	523
AfaI	GTAC	7 cut(s)	398, 501, 630, 707, 758, 1193, 2291
AfiI	CCNNNNNNNGG	2 cut(s)	110, 1568
AhdI	GACNNNNNGTC	1 cut(s)	352
AjnI	CCWGG	5 cut(s)	221, 407, 1537, 1561, 1731
AjuI	GAANNNNNNNTTGG	2 cut(s)	1698, 1730
AloI	GAACNNNNNNTCC	2 cut(s)	812, 844
Alw21I	GWGCWC	2 cut(s)	542, 599
Alw26I	GTCTC	4 cut(s)	789, 1046, 1261, 1812
Alw44I	GTGCAC	1 cut(s)	595
AlwI	GGATC	3 cut(s)	701, 1248, 2048
AoxI	GGCC	4 cut(s)	1230, 1361, 1702, 1735
ApaLI	GTGCAC	1 cut(s)	595
ApeKI	GCWGC	9 cut(s)	78, 480, 947, 962, 1045, 1610, 1721, 2107, 2149
ApoI	RAATTY	7 cut(s)	22, 712, 956, 1318, 1420, 1713, 2284
ArsI	GACNNNNNNTTYG	2 cut(s)	1197, 1229
AseI	ATTAAT	1 cut(s)	2120
Asp700I	GAANNNNTTC	2 cut(s)	26, 1215
AspLEI	GCGC	1 cut(s)	525
AspS9I	GGNCC	3 cut(s)	994, 1231, 1735
AsuHPI	GGTGA	2 cut(s)	1706, 2261
AvaII	GGWCC	1 cut(s)	994
BaeGI	GKGCMC	2 cut(s)	599, 2009
BalI	TGGCCA	1 cut(s)	1704
BanI	GGYRCC	1 cut(s)	522
BanII	GRGCYC	1 cut(s)	542
BbsI	GAAGAC	3 cut(s)	1461, 1956, 2232
Bbv12I	GWGCWC	2 cut(s)	542, 599
BbvI	GCAGC	9 cut(s)	90, 492, 934, 974, 1057, 1622, 1708, 2119, 2136
BccI	CCATC	6 cut(s)	112, 1283, 1499, 1746, 1797, 2240
BciT130I	CCWGG	5 cut(s)	223, 409, 1539, 1563, 1733
BciVI	GTATCC	1 cut(s)	1321
BclI	TGATCA	1 cut(s)	2251
BcoDI	GTCTC	4 cut(s)	789, 1046, 1261, 1812
BfaI	CTAG	5 cut(s)	90, 1656, 1797, 2013, 2163
BfmI	CTRYAG	1 cut(s)	2182
BfoI	RGCGCY	1 cut(s)	526
BfuI	GTATCC	1 cut(s)	1321
BlpI	GCTNAGC	1 cut(s)	1095
BmcAI	AGTACT	1 cut(s)	1193
Bme1390I	CCNGG	5 cut(s)	223, 409, 1539, 1563, 1733
Bme18I	GGWCC	1 cut(s)	994
BmeRI	GACNNNNNGTC	1 cut(s)	352
BmgT120I	GGNCC	3 cut(s)	994, 1231, 1735
BmiI	GGNNCC	3 cut(s)	115, 524, 1233
BmrFI	CCNGG	5 cut(s)	223, 409, 1539, 1563, 1733
BmsI	GCATC	7 cut(s)	256, 813, 1108, 1174, 1574, 1597, 1797
BoxI	GACNNNNGTC	1 cut(s)	1557
BpiI	GAAGAC	3 cut(s)	1461, 1956, 2232
BpmI	CTGGAG	1 cut(s)	1584
Bpu10I	CCTNAGC	1 cut(s)	1510
Bpu1102I	GCTNAGC	1 cut(s)	1095
BpuEI	CTTGAG	3 cut(s)	578, 1004, 1996
BsaBI	GATNNNNATC	1 cut(s)	2250
BsaHI	GRCGYC	1 cut(s)	523
BsaI	GGTCTC	2 cut(s)	1046, 1261
BsaJI	CCNNGG	3 cut(s)	815, 1138, 1732
BsaWI	WCCGGW	1 cut(s)	1789
BsaXI	ACNNNNNCTCC	6 cut(s)	25, 55, 812, 842, 1764, 1794
Bsc4I	CCNNNNNNNGG	2 cut(s)	110, 1568
Bse1I	ACTGG	4 cut(s)	352, 764, 790, 1257
Bse3DI	GCAATG	4 cut(s)	396, 759, 1089, 1620
Bse8I	GATNNNNATC	1 cut(s)	2250
BseBI	CCWGG	5 cut(s)	223, 409, 1539, 1563, 1733
BseDI	CCNNGG	3 cut(s)	815, 1138, 1732
BseGI	GGATG	5 cut(s)	1589, 1665, 1808, 1812, 1876
BseJI	GATNNNNATC	1 cut(s)	2250
BseLI	CCNNNNNNNGG	2 cut(s)	110, 1568
BseMI	GCAATG	4 cut(s)	396, 759, 1089, 1620
BseMII	CTCAG	7 cut(s)	86, 541, 798, 1109, 1272, 1524, 2167
BseNI	ACTGG	4 cut(s)	352, 764, 790, 1257
BseRI	GAGGAG	3 cut(s)	1581, 1593, 1785
BseSI	GKGCMC	2 cut(s)	599, 2009
BseXI	GCAGC	9 cut(s)	90, 492, 934, 974, 1057, 1622, 1708, 2119, 2136
BsgI	GTGCAG	2 cut(s)	720, 1284
BshFI	GGCC	4 cut(s)	1232, 1363, 1704, 1737
BshNI	GGYRCC	1 cut(s)	522
BsiHKAI	GWGCWC	2 cut(s)	542, 599
BsiSI	CCGG	3 cut(s)	12, 514, 1790
BslFI	GGGAC	1 cut(s)	947
BslI	CCNNNNNNNGG	2 cut(s)	110, 1568
BsmAI	GTCTC	4 cut(s)	789, 1046, 1261, 1812
BsmFI	GGGAC	1 cut(s)	947
BsmI	GAATGC	3 cut(s)	245, 2077, 2209
BsnI	GGCC	4 cut(s)	1232, 1363, 1704, 1737
Bso31I	GGTCTC	2 cut(s)	1046, 1261
Bsp1286I	GDGCHC	3 cut(s)	542, 599, 2009
Bsp143I	GATC	7 cut(s)	94, 693, 1063, 1240, 2040, 2045, 2251
Bsp1720I	GCTNAGC	1 cut(s)	1095
BspACI	CCGC	3 cut(s)	219, 980, 1414
BspANI	GGCC	4 cut(s)	1232, 1363, 1704, 1737
BspCNI	CTCAG	7 cut(s)	85, 540, 799, 1108, 1271, 1523, 2166
BspHI	TCATGA	1 cut(s)	1470
BspLI	GGNNCC	3 cut(s)	115, 524, 1233
BspPI	GGATC	3 cut(s)	701, 1248, 2048
BspT107I	GGYRCC	1 cut(s)	522
BspTNI	GGTCTC	2 cut(s)	1046, 1261
BsrDI	GCAATG	4 cut(s)	396, 759, 1089, 1620
BsrI	ACTGG	4 cut(s)	352, 764, 790, 1257
BssECI	CCNNGG	3 cut(s)	815, 1138, 1732
BssMI	GATC	7 cut(s)	94, 693, 1063, 1240, 2040, 2045, 2251
BssNI	GRCGYC	1 cut(s)	523
BssT1I	CCWWGG	2 cut(s)	815, 1138
Bst2UI	CCWGG	5 cut(s)	223, 409, 1539, 1563, 1733
Bst4CI	ACNGT	3 cut(s)	299, 940, 1378
Bst6I	CTCTTC	3 cut(s)	1109, 1658, 1855
BstACI	GRCGYC	1 cut(s)	523
BstAPI	GCANNNNNTGC	1 cut(s)	2213
BstC8I	GCNNGC	4 cut(s)	804, 945, 1653, 2112
BstDEI	CTNAG	7 cut(s)	72, 527, 807, 1095, 1258, 1510, 2153
BstENI	CCTNNNNNAGG	1 cut(s)	1566
BstF5I	GGATG	5 cut(s)	1589, 1665, 1808, 1812, 1876
BstH2I	RGCGCY	1 cut(s)	526
BstHHI	GCGC	1 cut(s)	525
BstKTI	GATC	7 cut(s)	97, 696, 1066, 1243, 2043, 2048, 2254
BstMAI	GTCTC	4 cut(s)	789, 1046, 1261, 1812
BstMBI	GATC	7 cut(s)	94, 693, 1063, 1240, 2040, 2045, 2251
BstMWI	GCNNNNNNNGC	5 cut(s)	489, 1593, 1652, 2013, 2213
BstNI	CCWGG	5 cut(s)	223, 409, 1539, 1563, 1733
BstNSI	RCATGY	4 cut(s)	511, 806, 847, 2077
BstPAI	GACNNNNGTC	1 cut(s)	1557
BstSCI	CCNGG	5 cut(s)	221, 407, 1537, 1561, 1731
BstSFI	CTRYAG	1 cut(s)	2182
BstSLI	GKGCMC	2 cut(s)	599, 2009
BstV1I	GCAGC	9 cut(s)	90, 492, 934, 974, 1057, 1622, 1708, 2119, 2136
BstV2I	GAAGAC	3 cut(s)	1461, 1956, 2232
BstX2I	RGATCY	1 cut(s)	1240
BstYI	RGATCY	1 cut(s)	1240
BsuI	GTATCC	1 cut(s)	1321
BsuRI	GGCC	4 cut(s)	1232, 1363, 1704, 1737
BtsCI	GGATG	5 cut(s)	1589, 1665, 1808, 1812, 1876
BtsI	GCAGTG	3 cut(s)	432, 708, 747
BtsIMutI	CAGTG	6 cut(s)	432, 708, 747, 783, 1424, 2009
Cac8I	GCNNGC	4 cut(s)	804, 945, 1653, 2112
CciI	TCATGA	1 cut(s)	1470
CfoI	GCGC	1 cut(s)	525
Cfr13I	GGNCC	3 cut(s)	994, 1231, 1735
CsiI	ACCWGGT	1 cut(s)	407
Csp6I	GTAC	7 cut(s)	397, 500, 629, 706, 757, 1192, 2290
CspCI	CAANNNNNGTGG	2 cut(s)	879, 914
CviQI	GTAC	7 cut(s)	397, 500, 629, 706, 757, 1192, 2290
DdeI	CTNAG	7 cut(s)	72, 527, 807, 1095, 1258, 1510, 2153
DinI	GGCGCC	1 cut(s)	524
DpnI	GATC	7 cut(s)	96, 695, 1065, 1242, 2042, 2047, 2253
DpnII	GATC	7 cut(s)	94, 693, 1063, 1240, 2040, 2045, 2251
DrdI	GACNNNNNNGTC	2 cut(s)	290, 2234
DriI	GACNNNNNGTC	1 cut(s)	352
DseDI	GACNNNNNNGTC	2 cut(s)	290, 2234
EaeI	YGGCCR	1 cut(s)	1702
Eam1104I	CTCTTC	3 cut(s)	1109, 1658, 1855
Eam1105I	GACNNNNNGTC	1 cut(s)	352
EarI	CTCTTC	3 cut(s)	1109, 1658, 1855
EciI	GGCGGA	1 cut(s)	208
Ecl136II	GAGCTC	1 cut(s)	540
Eco130I	CCWWGG	2 cut(s)	815, 1138
Eco24I	GRGCYC	1 cut(s)	542
Eco31I	GGTCTC	2 cut(s)	1046, 1261
Eco32I	GATATC	1 cut(s)	1132
Eco47I	GGWCC	1 cut(s)	994
Eco53kI	GAGCTC	1 cut(s)	540
Eco57I	CTGAAG	2 cut(s)	13, 936
EcoICRI	GAGCTC	1 cut(s)	540
EcoNI	CCTNNNNNAGG	1 cut(s)	1566
EcoO109I	RGGNCCY	1 cut(s)	1231
EcoRII	CCWGG	5 cut(s)	221, 407, 1537, 1561, 1731
EcoRV	GATATC	1 cut(s)	1132
EcoT14I	CCWWGG	2 cut(s)	815, 1138
EcoT22I	ATGCAT	1 cut(s)	2079
EcoT38I	GRGCYC	1 cut(s)	542
EgeI	GGCGCC	1 cut(s)	524
EheI	GGCGCC	1 cut(s)	524
ErhI	CCWWGG	2 cut(s)	815, 1138
FalI	AAGNNNNNCTT	4 cut(s)	11, 43, 255, 287
FaqI	GGGAC	1 cut(s)	947
FauNDI	CATATG	2 cut(s)	1013, 1150
FbaI	TGATCA	1 cut(s)	2251
FokI	GGATG	5 cut(s)	1596, 1672, 1815, 1819, 1883
FriOI	GRGCYC	1 cut(s)	542
FspBI	CTAG	5 cut(s)	90, 1656, 1797, 2013, 2163
GlaI	GCGC	1 cut(s)	524
GsuI	CTGGAG	1 cut(s)	1584
HaeII	RGCGCY	1 cut(s)	526
HaeIII	GGCC	4 cut(s)	1232, 1363, 1704, 1737
HapII	CCGG	3 cut(s)	12, 514, 1790
HhaI	GCGC	1 cut(s)	525
Hin1I	GRCGYC	1 cut(s)	523
Hin6I	GCGC	1 cut(s)	523
HinP1I	GCGC	1 cut(s)	523
HincII	GTYRAC	3 cut(s)	295, 370, 1783
HindII	GTYRAC	3 cut(s)	295, 370, 1783
HinfI	GANTC	7 cut(s)	120, 573, 589, 677, 924, 1410, 2135
HpaII	CCGG	3 cut(s)	12, 514, 1790
HphI	GGTGA	2 cut(s)	1706, 2261
Hpy166II	GTNNAC	6 cut(s)	295, 370, 468, 597, 706, 1783
Hpy188III	TCNNGA	9 cut(s)	31, 250, 344, 586, 1027, 1471, 1742, 1908, 2200
Hpy8I	GTNNAC	6 cut(s)	295, 370, 468, 597, 706, 1783
HpyAV	CCTTC	4 cut(s)	556, 1028, 1760, 1922
HpyCH4III	ACNGT	3 cut(s)	299, 940, 1378
HpyF10VI	GCNNNNNNNGC	5 cut(s)	489, 1593, 1652, 2013, 2213
HpyF3I	CTNAG	7 cut(s)	72, 527, 807, 1095, 1258, 1510, 2153
Hsp92I	GRCGYC	1 cut(s)	523
HspAI	GCGC	1 cut(s)	523
KasI	GGCGCC	1 cut(s)	522
Ksp22I	TGATCA	1 cut(s)	2251
Kzo9I	GATC	7 cut(s)	94, 693, 1063, 1240, 2040, 2045, 2251
LmnI	GCTCC	5 cut(s)	113, 144, 516, 545, 1772
Lsp1109I	GCAGC	9 cut(s)	90, 492, 934, 974, 1057, 1622, 1708, 2119, 2136
LweI	GCATC	7 cut(s)	256, 813, 1108, 1174, 1574, 1597, 1797
MabI	ACCWGGT	1 cut(s)	407
MaeI	CTAG	5 cut(s)	90, 1656, 1797, 2013, 2163
MaeIII	GTNAC	7 cut(s)	192, 613, 745, 1005, 2024, 2062, 2299
MalI	GATC	7 cut(s)	96, 695, 1065, 1242, 2042, 2047, 2253
MboI	GATC	7 cut(s)	94, 693, 1063, 1240, 2040, 2045, 2251
MfeI	CAATTG	4 cut(s)	1166, 1555, 1633, 1921
MflI	RGATCY	1 cut(s)	1240
MhlI	GDGCHC	3 cut(s)	542, 599, 2009
MlsI	TGGCCA	1 cut(s)	1704
MluNI	TGGCCA	1 cut(s)	1704
Mly113I	GGCGCC	1 cut(s)	523
MlyI	GAGTC	2 cut(s)	583, 1404
MmeI	TCCRAC	1 cut(s)	2079
Mox20I	TGGCCA	1 cut(s)	1704
Mph1103I	ATGCAT	1 cut(s)	2079
MroXI	GAANNNNTTC	2 cut(s)	26, 1215
MscI	TGGCCA	1 cut(s)	1704
MseI	TTAA	5 cut(s)	495, 1274, 1544, 1844, 2120
MslI	CAYNNNNRTG	6 cut(s)	611, 752, 1012, 1484, 1605, 2002
Msp20I	TGGCCA	1 cut(s)	1704
MspI	CCGG	3 cut(s)	12, 514, 1790
MspR9I	CCNGG	5 cut(s)	223, 409, 1539, 1563, 1733
MunI	CAATTG	4 cut(s)	1166, 1555, 1633, 1921
Mva1269I	GAATGC	3 cut(s)	245, 2077, 2209
MvaI	CCWGG	5 cut(s)	223, 409, 1539, 1563, 1733
MwoI	GCNNNNNNNGC	5 cut(s)	489, 1593, 1652, 2013, 2213
NarI	GGCGCC	1 cut(s)	523
NdeI	CATATG	2 cut(s)	1013, 1150
NdeII	GATC	7 cut(s)	94, 693, 1063, 1240, 2040, 2045, 2251
NlaIV	GGNNCC	3 cut(s)	115, 524, 1233
NmeAIII	GCCGAG	1 cut(s)	1213
NmuCI	GTSAC	5 cut(s)	613, 745, 1005, 2024, 2299
NsiI	ATGCAT	1 cut(s)	2079
NspI	RCATGY	4 cut(s)	511, 806, 847, 2077
PaeI	GCATGC	1 cut(s)	806
PagI	TCATGA	1 cut(s)	1470
PctI	GAATGC	3 cut(s)	245, 2077, 2209
PdmI	GAANNNNTTC	2 cut(s)	26, 1215
PfeI	GAWTC	5 cut(s)	120, 573, 677, 924, 2135
PflMI	CCANNNNNTGG	1 cut(s)	110
PfoI	TCCNGGA	1 cut(s)	1561
PleI	GAGTC	2 cut(s)	583, 1404
PluTI	GGCGCC	1 cut(s)	526
PpsI	GAGTC	2 cut(s)	583, 1404
PshAI	GACNNNNGTC	1 cut(s)	1557
PshBI	ATTAAT	1 cut(s)	2120
Psp124BI	GAGCTC	1 cut(s)	542
Psp6I	CCWGG	5 cut(s)	221, 407, 1537, 1561, 1731
PspGI	CCWGG	5 cut(s)	221, 407, 1537, 1561, 1731
PspN4I	GGNNCC	3 cut(s)	115, 524, 1233
PspPI	GGNCC	3 cut(s)	994, 1231, 1735
PsuI	RGATCY	1 cut(s)	1240
RsaI	GTAC	7 cut(s)	398, 501, 630, 707, 758, 1193, 2291
RsaNI	GTAC	7 cut(s)	397, 500, 629, 706, 757, 1192, 2290
RseI	CAYNNNNRTG	6 cut(s)	611, 752, 1012, 1484, 1605, 2002
SacI	GAGCTC	1 cut(s)	542
SaqAI	TTAA	5 cut(s)	495, 1274, 1544, 1844, 2120
Sau3AI	GATC	7 cut(s)	94, 693, 1063, 1240, 2040, 2045, 2251
Sau96I	GGNCC	3 cut(s)	994, 1231, 1735
ScaI	AGTACT	1 cut(s)	1193
SchI	GAGTC	2 cut(s)	583, 1404
ScrFI	CCNGG	5 cut(s)	223, 409, 1539, 1563, 1733
SduI	GDGCHC	3 cut(s)	542, 599, 2009
SexAI	ACCWGGT	1 cut(s)	407
SfaNI	GCATC	7 cut(s)	256, 813, 1108, 1174, 1574, 1597, 1797
SfcI	CTRYAG	1 cut(s)	2182
SfoI	GGCGCC	1 cut(s)	524
SinI	GGWCC	1 cut(s)	994
SmiMI	CAYNNNNRTG	6 cut(s)	611, 752, 1012, 1484, 1605, 2002
SmlI	CTYRAG	3 cut(s)	557, 1019, 1975
SmoI	CTYRAG	3 cut(s)	557, 1019, 1975
SphI	GCATGC	1 cut(s)	806
SsiI	CCGC	3 cut(s)	219, 980, 1414
SspDI	GGCGCC	1 cut(s)	522
SspI	AATATT	2 cut(s)	1285, 1404
SspMI	CTAG	5 cut(s)	90, 1656, 1797, 2013, 2163
SstI	GAGCTC	1 cut(s)	542
StyD4I	CCNGG	5 cut(s)	221, 407, 1537, 1561, 1731
StyI	CCWWGG	2 cut(s)	815, 1138
TaaI	ACNGT	3 cut(s)	299, 940, 1378
TaqI	TCGA	8 cut(s)	237, 282, 345, 420, 571, 831, 908, 1128
TatI	WGTACW	4 cut(s)	628, 756, 1191, 2289
TauI	GCSGC	1 cut(s)	982
TfiI	GAWTC	5 cut(s)	120, 573, 677, 924, 2135
Tru1I	TTAA	5 cut(s)	495, 1274, 1544, 1844, 2120
Tru9I	TTAA	5 cut(s)	495, 1274, 1544, 1844, 2120
TscAI	CASTG	6 cut(s)	439, 708, 754, 790, 1431, 2009
TseFI	GTSAC	5 cut(s)	613, 745, 1005, 2024, 2299
TseI	GCWGC	9 cut(s)	78, 480, 947, 962, 1045, 1610, 1721, 2107, 2149
Tsp45I	GTSAC	5 cut(s)	613, 745, 1005, 2024, 2299
TspGWI	ACGGA	1 cut(s)	1253
TspRI	CASTG	6 cut(s)	439, 708, 754, 790, 1431, 2009
Van91I	CCANNNNNTGG	1 cut(s)	110
VneI	GTGCAC	1 cut(s)	595
VpaK11BI	GGWCC	1 cut(s)	994
VspI	ATTAAT	1 cut(s)	2120
XagI	CCTNNNNNAGG	1 cut(s)	1566
XapI	RAATTY	7 cut(s)	22, 712, 956, 1318, 1420, 1713, 2284
XceI	RCATGY	4 cut(s)	511, 806, 847, 2077
XmnI	GAANNNNTTC	2 cut(s)	26, 1215
XspI	CTAG	5 cut(s)	90, 1656, 1797, 2013, 2163
ZrmI	AGTACT	1 cut(s)	1193
Zsp2I	ATGCAT	1 cut(s)	2079

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.