Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0008964

Physical Location & Seq

Genomic Coordinates Reverse (-)

41824644 .. 41829339

Gene Structure 4696 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1284400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2286 bp

ATGACATTTTCGTGCGCAAAAAACTTCTTCCGAAGGTTCTGTATTGATGAGTACCAACTAGACACTGTTGCTCAGAGCAGCTTCTTCTCTACTGATCTTCTGCCTTCCCTTGGTGCCAGAATCAACCAGTCTACTAAGCTCCGGAAATACATTATCTCGCCGTATAATCCTCGTTATAGGACGTGGGAGATGGTACTTGTTCTTCTAGTCATCTACTCTGCGTGGATTTGCCCGTTCGAGTTTGCATTTCTCCCTTACAAGCAGGATGCTCTTTTCATCGTTGACAACATTGTCAACGGCTTCTTTGGCATTGACATCATTCTCACTTTCTTTGTTGCCTATCTCGACAGCCGCTCTTACCTTCTTGTTGACAATCCAAAGCAAATCGCAATGAGGTACTTATCAACCTGGTTTCTTTTTGACGTGTGTTCCACTGCACCATTTCAGTCTATCAGCCTCCTCTTCACAAATCGCGGCAGCGAACTTGGGTTTAAAGTACTCAACATGCTCCGCCTCTGGCGCCTCAGACGAGTCAGCTACCTCTTTGCAAGACTTGAGAAGGACATCCGATTCAACTACTTCTGGATTCGCTGCACGAAGCTCATTTTTGTAACCCTTTTTGCAGTGCACTGCGCCGGATGCATCAACTATCTGATAGCAGACCGGTATCCTGATCCGAAAAGAACCTGGATCGGTGCAGTGTACCCGGATTTCAAACAAGACGGTCTCTGGAACAGATACGTTACTTCAATATACTGGTCCATCACAACACTAACAACCACTGGCTATGGAGATTTGCATGCTGAGAACCCTAGAGAGATGCTGTTTGATATTTTCTACATGCTCTTCAACTTGGGACTGAATTCTTACCTCATAGGAAACATGACAAATCTTGTAGTTCATTGGACCAGCAGAACCAGAATCTTTAGGGACACAGTGAGAGCTGCAACAGAATTTGCAGCAAGAAATGACTTGCCCCCAAGGATTCAGGACCAAATGCTCTCACATATATGCCTCAAGTTCAAGACAGAAGGACTGAAGCAGCAAGAGACCTTAAATGGTCTCCCAAAAGCCCTTCGTTCTAGCATTGCCCAACATCTCTTCTTCCCCATCATTCAAAACATCTACCTCTTTCAAGGAGTTTCTCATGAATTCCTCTTTCAATTGGTTTCAGAAATAGATGCAGAGTATTTTCCGCCCAAGGAAGACGTAATTCTGCAAAACGAGGCTCCGACCGATCTATACATACTGGTTTCCGGTGCGGCGGATCTGGTGTCCAATATTGATGGGCAAGATCAAGTTATTGGAAAGGCTACTGCGGGGGATACTTTGGGGGAAATCGGAGTATTATGTCATAGGCCGCAGCCTTTCACCATTCGGACAACCGAACTTTCCCAAATATTACGACTCCGCAGAAGTTCACTCATGACCACTATACAAGCAAACAGGGATGACGAGCAGATTATCATGAACAACATTTTTCTGAAACTGAAGGAACAAGAAGGGTTGGGCTGTGAATATCCACATACCGAAGGATGCTGTTCTTGCGCGGGGTGCAAAGATAACTCGCATCAAGAACCATCAATGCAGGAAGCAAGGAATGATTTGTTTACAGGTTCAGAGACTATAGAAAAGAGCGAAATAGGCAAAGCTGATATTTCAACTAGATGTGCAATGGATGTCAGCACGATGGCTGAGGATGGCCAGACGGCTCTTCACACAGCTGTTTGCCAGGGAGATTTGGAAATGGTCAAAATTTTGGTCGAAGGAGGAGCAAATGTAAACAAACCAGATGCCAGAGGATGGACGCCGAAAGATCTGGCACAACAGCAAGGAAACAAGAGCATAACTGACCTCTTACGACGATATGAGAATAGGAGAGCAGCCGAACATAGAATACAGTTTATTGAACTGGAAACATCTGAAATCACCAGGAATTGTAAAAGAAATTCCAAAAGACACGAGGGTGCCCAATTTTCCCACTCTCACCAGAAAGAAGTACCCATAAAGTGCTACCCGAGCAATTCTGGCCCAGATAGAGAAGGGATGAGATCAATCAACAATAGAGTAACTATCCACATGCATTTTCAGAATGGAACTGCGTCGGAAAGGCAGCTTGCAAAGTTAATAATCCTACCTGATTCGATCGAAGAGCTGCTCAGAGTTGCCAGTGATAAGTTCGGAGGACACAAACCTACAAAAGTTGTGAACGCAGAGAATGCAGAAATAGATGACATAAGTGTTGTTCAAGATGGTGATCACCTGTTTCTTCTCCATAACGACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005216 GO:0005242 GO:0005244 GO:0005249 GO:0005261 GO:0005267 GO:0005488 GO:0005515 GO:0005575 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0007623 GO:0008150 GO:0008324 GO:0009314 GO:0009416 GO:0009628 GO:0009642 GO:0009644 GO:0009987 GO:0010118 GO:0015075 GO:0015077 GO:0015079 GO:0015267 GO:0015276 GO:0015318 GO:0015672 GO:0016020 GO:0016021 GO:0022803 GO:0022832 GO:0022834 GO:0022836 GO:0022838 GO:0022839 GO:0022843 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042391 GO:0042802 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0048511 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0065007 GO:0065008 GO:0071804 GO:0071805 GO:0071944 GO:0098655 GO:0098660 GO:0098662 GO:0099094

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ank_2 Ion_trans KHA cNMP_binding

Protein Analysis

762

Amino Acids

87.15

Weight (kDa)

6.37

Isoelectric Point (pI)

42.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ion_trans	PF00520	59 - 306	2.2e-42	Ion transport protein
Ion_trans_2	PF07885	234 - 299	8.8e-12	Ion channel
cNMP_binding	PF00027	397 - 481	1.2e-14	Cyclic nucleotide-binding domain
Ank_2	PF12796	561 - 621	3.5e-09	Ankyrin repeats (3 copies)
Ank_5	PF13857	562 - 608	1.4e-06	Ankyrin repeats (many copies)
Ank	PF00023	567 - 598	4.3e-08	Ankyrin repeat
KHA	PF11834	689 - 757	1.7e-24	KHA, dimerisation domain of potassium ion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0008964)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G18290 AT4G18290 AT5G46240
fragaria_vesca	FvH4_3g05650 FvH4_3g05650
malus_domestica	MD05G1284400.v1.1 MD10G1262800.v1.1
prunus_persica	Prupe.4G080000_v2.0.a1 Prupe.4G080000_v2.0.a1
pyrus_communis	pycom05g26360
rosa_chinensis	RchiOBHm_Chr5g0013991
rosa_laevigata	RLG00000032060
rosa_multiflora	Rmu_co8232297.1_g000001 Rmu_co8474133.1_g000001 Rmu_sc0005803.1_g000012
rosa_roxburghii	Rroxscaffold_1G00062720
rosa_rugosa	Rorug05G0013900
rosa_samantha	Rh5AG108400 Rh5BG105200 Rh5CG117000 Rh5DG103900
rosa_wichuraiana	Rw5G009460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	290
Acc16I	TGCGCA	1 cut(s)	16
AccB1I	GGYRCC	3 cut(s)	113, 519, 1967
AccB7I	CCANNNNNTGG	1 cut(s)	1803
AccBSI	CCGCTC	1 cut(s)	354
AccI	GTMKAC	1 cut(s)	131
AccII	CGCG	2 cut(s)	474, 1550
AccIII	TCCGGA	1 cut(s)	141
AclWI	GGATC	3 cut(s)	668, 698, 1275
AcoI	YGGCCR	1 cut(s)	1702
AcsI	RAATTY	5 cut(s)	862, 953, 1151, 1755, 1948
AcuI	CTGAAG	2 cut(s)	1058, 1511
AcyI	GRCGYC	2 cut(s)	520, 1808
AfaI	GTAC	6 cut(s)	53, 195, 398, 498, 704, 2001
AfiI	CCNNNNNNNGG	2 cut(s)	110, 1803
AflIII	ACRYGT	1 cut(s)	423
AgeI	ACCGGT	1 cut(s)	663
AjiI	CACGTC	2 cut(s)	183, 424
AjnI	CCWGG	4 cut(s)	407, 686, 1731, 1931
AleI	CACNNNNGTG	1 cut(s)	1965
AluBI	AGCT	9 cut(s)	81, 139, 537, 601, 944, 1652, 1724, 2116, 2155
AluI	AGCT	9 cut(s)	81, 139, 537, 601, 944, 1652, 1724, 2116, 2155
Alw21I	GWGCWC	1 cut(s)	630
Alw26I	GTCTC	4 cut(s)	731, 1043, 1067, 1616
Alw44I	GTGCAC	1 cut(s)	626
AlwI	GGATC	3 cut(s)	668, 698, 1275
Ama87I	CYCGRG	1 cut(s)	2017
Aor13HI	TCCGGA	1 cut(s)	141
AoxI	GGCC	3 cut(s)	1358, 1702, 2029
ApaLI	GTGCAC	1 cut(s)	626
ApoI	RAATTY	5 cut(s)	862, 953, 1151, 1755, 1948
ArsI	GACNNNNNNTTYG	2 cut(s)	1746, 1778
AsiGI	ACCGGT	1 cut(s)	663
AspLEI	GCGC	4 cut(s)	17, 522, 635, 1550
AspS9I	GGNCC	4 cut(s)	759, 906, 991, 2030
AsuC2I	CCSGG	1 cut(s)	707
AsuHPI	GGTGA	5 cut(s)	1363, 1921, 1979, 2252, 2267
AvaI	CYCGRG	1 cut(s)	2017
AvaII	GGWCC	3 cut(s)	759, 906, 991
BaeGI	GKGCMC	2 cut(s)	630, 1972
BalI	TGGCCA	1 cut(s)	1704
BanI	GGYRCC	3 cut(s)	113, 519, 1967
BarI	GAAGNNNNNNTAC	2 cut(s)	186, 218
BauI	CACGAG	1 cut(s)	1961
BbsI	GAAGAC	1 cut(s)	1212
Bbv12I	GWGCWC	1 cut(s)	630
BbvCI	CCTCAGC	1 cut(s)	1695
BccI	CCATC	9 cut(s)	184, 770, 1118, 1280, 1588, 1684, 1694, 1797, 2246
BceAI	ACGGC	3 cut(s)	145, 313, 1725
BciT130I	CCWGG	4 cut(s)	409, 688, 1733, 1933
BciVI	GTATCC	2 cut(s)	678, 1318
BclI	TGATCA	1 cut(s)	2257
BcnI	CCSGG	1 cut(s)	707
BcoDI	GTCTC	4 cut(s)	731, 1043, 1067, 1616
BfaI	CTAG	5 cut(s)	59, 206, 813, 1083, 1665
BfmI	CTRYAG	1 cut(s)	1626
BfoI	RGCGCY	1 cut(s)	523
BfuI	GTATCC	2 cut(s)	678, 1318
BglII	AGATCT	1 cut(s)	1816
BmcAI	AGTACT	1 cut(s)	498
Bme1390I	CCNGG	5 cut(s)	409, 688, 707, 1733, 1933
Bme18I	GGWCC	3 cut(s)	759, 906, 991
BmeT110I	CYCGRG	1 cut(s)	2017
BmgBI	CACGTC	2 cut(s)	183, 424
BmgT120I	GGNCC	4 cut(s)	759, 906, 991, 2030
BmiI	GGNNCC	4 cut(s)	115, 521, 1230, 1969
BmrFI	CCNGG	5 cut(s)	409, 688, 707, 1733, 1933
BmsI	GCATC	8 cut(s)	256, 629, 651, 810, 1171, 1526, 1579, 1783
BpiI	GAAGAC	1 cut(s)	1212
Bpu10I	CCTNAGC	1 cut(s)	1695
BpuEI	CTTGAG	2 cut(s)	575, 1001
BpuMI	CCSGG	1 cut(s)	707
BsaBI	GATNNNNATC	1 cut(s)	2256
BsaHI	GRCGYC	2 cut(s)	520, 1808
BsaI	GGTCTC	3 cut(s)	731, 1043, 1067
BsaJI	CCNNGG	4 cut(s)	109, 980, 1200, 1732
BsaWI	WCCGGW	3 cut(s)	141, 663, 1256
BsaXI	ACNNNNNCTCC	2 cut(s)	1764, 1794
Bsc4I	CCNNNNNNNGG	2 cut(s)	110, 1803
Bse118I	RCCGGY	1 cut(s)	663
Bse1I	ACTGG	6 cut(s)	127, 761, 787, 1254, 1917, 2169
Bse3DI	GCAATG	3 cut(s)	396, 1086, 1680
Bse8I	GATNNNNATC	1 cut(s)	2256
BseAI	TCCGGA	1 cut(s)	141
BseBI	CCWGG	4 cut(s)	409, 688, 1733, 1933
BseDI	CCNNGG	4 cut(s)	109, 980, 1200, 1732
BseGI	GGATG	9 cut(s)	271, 564, 644, 1456, 1541, 1684, 1705, 1808, 2052
BseJI	GATNNNNATC	1 cut(s)	2256
BseLI	CCNNNNNNNGG	2 cut(s)	110, 1803
BseMI	GCAATG	3 cut(s)	396, 1086, 1680
BseMII	CTCAG	5 cut(s)	86, 538, 795, 1686, 2173
BseNI	ACTGG	6 cut(s)	127, 761, 787, 1254, 1917, 2169
BseRI	GAGGAG	2 cut(s)	449, 1785
BseSI	GKGCMC	2 cut(s)	630, 1972
BsgI	GTGCAG	3 cut(s)	420, 577, 717
Bsh1236I	CGCG	2 cut(s)	474, 1550
Bsh1285I	CGRYCG	2 cut(s)	1236, 2148
BshFI	GGCC	3 cut(s)	1360, 1704, 2031
BshNI	GGYRCC	3 cut(s)	113, 519, 1967
BshTI	ACCGGT	1 cut(s)	663
BsiEI	CGRYCG	2 cut(s)	1236, 2148
BsiHKAI	GWGCWC	1 cut(s)	630
BsiHKCI	CYCGRG	1 cut(s)	2017
BsiSI	CCGG	5 cut(s)	142, 636, 664, 707, 1257
BslFI	GGGAC	2 cut(s)	870, 944
BslI	CCNNNNNNNGG	2 cut(s)	110, 1803
BsmAI	GTCTC	4 cut(s)	731, 1043, 1067, 1616
BsmFI	GGGAC	2 cut(s)	870, 944
BsmI	GAATGC	1 cut(s)	2224
BsnI	GGCC	3 cut(s)	1360, 1704, 2031
Bso31I	GGTCTC	3 cut(s)	731, 1043, 1067
BsoBI	CYCGRG	1 cut(s)	2017
Bsp1286I	GDGCHC	2 cut(s)	630, 1972
Bsp13I	TCCGGA	1 cut(s)	141
BspANI	GGCC	3 cut(s)	1360, 1704, 2031
BspCNI	CTCAG	5 cut(s)	85, 537, 796, 1687, 2172
BspEI	TCCGGA	1 cut(s)	141
BspFNI	CGCG	2 cut(s)	474, 1550
BspHI	TCATGA	3 cut(s)	1147, 1425, 1467
BspLI	GGNNCC	4 cut(s)	115, 521, 1230, 1969
BspPI	GGATC	3 cut(s)	668, 698, 1275
BspQI	GCTCTTC	3 cut(s)	851, 1719, 2145
BspT107I	GGYRCC	3 cut(s)	113, 519, 1967
BspTNI	GGTCTC	3 cut(s)	731, 1043, 1067
BsrBI	CCGCTC	1 cut(s)	354
BsrDI	GCAATG	3 cut(s)	396, 1086, 1680
BsrFI	RCCGGY	1 cut(s)	663
BsrI	ACTGG	6 cut(s)	127, 761, 787, 1254, 1917, 2169
BssAI	RCCGGY	1 cut(s)	663
BssECI	CCNNGG	4 cut(s)	109, 980, 1200, 1732
BssNI	GRCGYC	2 cut(s)	520, 1808
BssSI	CACGAG	1 cut(s)	1961
BssT1I	CCWWGG	3 cut(s)	109, 980, 1200
Bst2BI	CACGAG	1 cut(s)	1961
Bst2UI	CCWGG	4 cut(s)	409, 688, 1733, 1933
Bst4CI	ACNGT	4 cut(s)	67, 725, 937, 1902
Bst6I	CTCTTC	5 cut(s)	467, 851, 1106, 1719, 2145
BstACI	GRCGYC	2 cut(s)	520, 1808
BstAPI	GCANNNNNTGC	1 cut(s)	2219
BstC8I	GCNNGC	2 cut(s)	801, 2118
BstDEI	CTNAG	6 cut(s)	72, 135, 524, 804, 1695, 2159
BstF5I	GGATG	9 cut(s)	271, 564, 644, 1456, 1541, 1684, 1705, 1808, 2052
BstFNI	CGCG	2 cut(s)	474, 1550
BstH2I	RGCGCY	1 cut(s)	523
BstHHI	GCGC	4 cut(s)	17, 522, 635, 1550
BstMAI	GTCTC	4 cut(s)	731, 1043, 1067, 1616
BstMCI	CGRYCG	2 cut(s)	1236, 2148
BstMWI	GCNNNNNNNGC	9 cut(s)	306, 519, 639, 1545, 1554, 1644, 2019, 2028, 2219
BstNI	CCWGG	4 cut(s)	409, 688, 1733, 1933
BstNSI	RCATGY	4 cut(s)	508, 803, 844, 2083
BstSCI	CCNGG	5 cut(s)	407, 686, 705, 1731, 1931
BstSFI	CTRYAG	1 cut(s)	1626
BstSLI	GKGCMC	2 cut(s)	630, 1972
BstUI	CGCG	2 cut(s)	474, 1550
BstV2I	GAAGAC	1 cut(s)	1212
BstX2I	RGATCY	2 cut(s)	1267, 1816
BstYI	RGATCY	2 cut(s)	1267, 1816
BsuI	GTATCC	2 cut(s)	678, 1318
BsuRI	GGCC	3 cut(s)	1360, 1704, 2031
BtrI	CACGTC	2 cut(s)	183, 424
BtsCI	GGATG	9 cut(s)	271, 564, 644, 1456, 1541, 1684, 1705, 1808, 2052
BtsI	GCAGTG	4 cut(s)	432, 628, 630, 705
BtsIMutI	CAGTG	8 cut(s)	63, 432, 628, 630, 705, 780, 942, 2176
Cac8I	GCNNGC	2 cut(s)	801, 2118
CciI	TCATGA	3 cut(s)	1147, 1425, 1467
CfoI	GCGC	4 cut(s)	17, 522, 635, 1550
Cfr10I	RCCGGY	1 cut(s)	663
Cfr13I	GGNCC	4 cut(s)	759, 906, 991, 2030
CseI	GACGC	2 cut(s)	1816, 2091
CsiI	ACCWGGT	1 cut(s)	407
Csp6I	GTAC	6 cut(s)	52, 194, 397, 497, 703, 2000
CspAI	ACCGGT	1 cut(s)	663
CviAII	CATG	8 cut(s)	505, 800, 841, 883, 1148, 1426, 1468, 2080
CviQI	GTAC	6 cut(s)	52, 194, 397, 497, 703, 2000
DdeI	CTNAG	6 cut(s)	72, 135, 524, 804, 1695, 2159
DinI	GGCGCC	1 cut(s)	521
DraI	TTTAAA	1 cut(s)	493
DrdI	GACNNNNNNGTC	1 cut(s)	290
DseDI	GACNNNNNNGTC	1 cut(s)	290
EaeI	YGGCCR	1 cut(s)	1702
Eam1104I	CTCTTC	5 cut(s)	467, 851, 1106, 1719, 2145
EarI	CTCTTC	5 cut(s)	467, 851, 1106, 1719, 2145
EciI	GGCGGA	3 cut(s)	500, 1185, 1280
Eco130I	CCWWGG	3 cut(s)	109, 980, 1200
Eco31I	GGTCTC	3 cut(s)	731, 1043, 1067
Eco47I	GGWCC	3 cut(s)	759, 906, 991
Eco57I	CTGAAG	2 cut(s)	1058, 1511
Eco88I	CYCGRG	1 cut(s)	2017
EcoRI	GAATTC	2 cut(s)	862, 1151
EcoRII	CCWGG	4 cut(s)	407, 686, 1731, 1931
EcoT14I	CCWWGG	3 cut(s)	109, 980, 1200
EcoT22I	ATGCAT	2 cut(s)	644, 2085
EgeI	GGCGCC	1 cut(s)	521
EheI	GGCGCC	1 cut(s)	521
ErhI	CCWWGG	3 cut(s)	109, 980, 1200
FaeI	CATG	8 cut(s)	508, 803, 844, 886, 1151, 1429, 1471, 2083
FaqI	GGGAC	2 cut(s)	870, 944
FatI	CATG	8 cut(s)	504, 799, 840, 882, 1147, 1425, 1467, 2079
FauI	CCCGC	2 cut(s)	1312, 1543
FbaI	TGATCA	1 cut(s)	2257
FblI	GTMKAC	1 cut(s)	131
FokI	GGATG	9 cut(s)	278, 551, 651, 1463, 1548, 1691, 1712, 1815, 2059
FspBI	CTAG	5 cut(s)	59, 206, 813, 1083, 1665
FspI	TGCGCA	1 cut(s)	16
GlaI	GCGC	4 cut(s)	16, 521, 634, 1549
HaeII	RGCGCY	1 cut(s)	523
HaeIII	GGCC	3 cut(s)	1360, 1704, 2031
HapII	CCGG	5 cut(s)	142, 636, 664, 707, 1257
HgaI	GACGC	2 cut(s)	1816, 2091
HhaI	GCGC	4 cut(s)	17, 522, 635, 1550
Hin1I	GRCGYC	2 cut(s)	520, 1808
Hin1II	CATG	8 cut(s)	508, 803, 844, 886, 1151, 1429, 1471, 2083
Hin6I	GCGC	4 cut(s)	15, 520, 633, 1548
HinP1I	GCGC	4 cut(s)	15, 520, 633, 1548
HincII	GTYRAC	3 cut(s)	283, 295, 370
HindII	GTYRAC	3 cut(s)	283, 295, 370
HinfI	GANTC	8 cut(s)	120, 531, 570, 586, 921, 985, 1407, 2141
HpaII	CCGG	5 cut(s)	142, 636, 664, 707, 1257
HphI	GGTGA	5 cut(s)	1363, 1921, 1979, 2252, 2267
Hpy99I	CGWCG	2 cut(s)	1866, 2107
HpyCH4III	ACNGT	4 cut(s)	67, 725, 937, 1902
HpyCH4IV	ACGT	4 cut(s)	182, 423, 741, 1209
HpyF10VI	GCNNNNNNNGC	9 cut(s)	306, 519, 639, 1545, 1554, 1644, 2019, 2028, 2219
HpyF3I	CTNAG	6 cut(s)	72, 135, 524, 804, 1695, 2159
HpySE526I	ACGT	4 cut(s)	182, 423, 741, 1209
Hsp92I	GRCGYC	2 cut(s)	520, 1808
Hsp92II	CATG	8 cut(s)	508, 803, 844, 886, 1151, 1429, 1471, 2083
HspAI	GCGC	4 cut(s)	15, 520, 633, 1548
KasI	GGCGCC	1 cut(s)	519
Kpn2I	TCCGGA	1 cut(s)	141
Ksp22I	TGATCA	1 cut(s)	2257
LguI	GCTCTTC	3 cut(s)	851, 1719, 2145
LmnI	GCTCC	4 cut(s)	144, 513, 1234, 1772
LweI	GCATC	8 cut(s)	256, 629, 651, 810, 1171, 1526, 1579, 1783
MabI	ACCWGGT	1 cut(s)	407
MaeI	CTAG	5 cut(s)	59, 206, 813, 1083, 1665
MaeII	ACGT	4 cut(s)	182, 423, 741, 1209
MaeIII	GTNAC	3 cut(s)	610, 742, 2068
MbiI	CCGCTC	1 cut(s)	354
MfeI	CAATTG	1 cut(s)	1163
MflI	RGATCY	2 cut(s)	1267, 1816
MhlI	GDGCHC	2 cut(s)	630, 1972
MlsI	TGGCCA	1 cut(s)	1704
MluNI	TGGCCA	1 cut(s)	1704
Mly113I	GGCGCC	1 cut(s)	520
MlyI	GAGTC	2 cut(s)	540, 1401
MmeI	TCCRAC	2 cut(s)	1256, 2085
Mox20I	TGGCCA	1 cut(s)	1704
Mph1103I	ATGCAT	2 cut(s)	644, 2085
MroI	TCCGGA	1 cut(s)	141
MscI	TGGCCA	1 cut(s)	1704
MseI	TTAA	3 cut(s)	492, 1055, 2126
MslI	CAYNNNNRTG	3 cut(s)	10, 1009, 1965
Msp20I	TGGCCA	1 cut(s)	1704
MspA1I	CMGCKG	1 cut(s)	1724
MspI	CCGG	5 cut(s)	142, 636, 664, 707, 1257
MspR9I	CCNGG	5 cut(s)	409, 688, 707, 1733, 1933
MunI	CAATTG	1 cut(s)	1163
Mva1269I	GAATGC	1 cut(s)	2224
MvaI	CCWGG	4 cut(s)	409, 688, 1733, 1933
MvnI	CGCG	2 cut(s)	474, 1550
MwoI	GCNNNNNNNGC	9 cut(s)	306, 519, 639, 1545, 1554, 1644, 2019, 2028, 2219
NarI	GGCGCC	1 cut(s)	520
NciI	CCSGG	1 cut(s)	707
NlaIII	CATG	8 cut(s)	508, 803, 844, 886, 1151, 1429, 1471, 2083
NlaIV	GGNNCC	4 cut(s)	115, 521, 1230, 1969
NsbI	TGCGCA	1 cut(s)	16
NsiI	ATGCAT	2 cut(s)	644, 2085
NspI	RCATGY	4 cut(s)	508, 803, 844, 2083
OliI	CACNNNNGTG	1 cut(s)	1965
PaeI	GCATGC	1 cut(s)	803
PagI	TCATGA	3 cut(s)	1147, 1425, 1467
PciSI	GCTCTTC	3 cut(s)	851, 1719, 2145
PctI	GAATGC	1 cut(s)	2224
PfeI	GAWTC	6 cut(s)	120, 570, 586, 921, 985, 2141
PflMI	CCANNNNNTGG	1 cut(s)	1803
PinAI	ACCGGT	1 cut(s)	663
Ple19I	CGATCG	1 cut(s)	2148
PleI	GAGTC	2 cut(s)	539, 1401
PluTI	GGCGCC	1 cut(s)	523
PpsI	GAGTC	2 cut(s)	539, 1401
Psp6I	CCWGG	4 cut(s)	407, 686, 1731, 1931
PspGI	CCWGG	4 cut(s)	407, 686, 1731, 1931
PspN4I	GGNNCC	4 cut(s)	115, 521, 1230, 1969
PspPI	GGNCC	4 cut(s)	759, 906, 991, 2030
PsrI	GAACNNNNNNTAC	2 cut(s)	1881, 1913
PsuI	RGATCY	2 cut(s)	1267, 1816
PvuI	CGATCG	1 cut(s)	2148
PvuII	CAGCTG	1 cut(s)	1724
RsaI	GTAC	6 cut(s)	53, 195, 398, 498, 704, 2001
RsaNI	GTAC	6 cut(s)	52, 194, 397, 497, 703, 2000
RseI	CAYNNNNRTG	3 cut(s)	10, 1009, 1965
SapI	GCTCTTC	3 cut(s)	851, 1719, 2145
SaqAI	TTAA	3 cut(s)	492, 1055, 2126
Sau96I	GGNCC	4 cut(s)	759, 906, 991, 2030
ScaI	AGTACT	1 cut(s)	498
SchI	GAGTC	2 cut(s)	540, 1401
ScrFI	CCNGG	5 cut(s)	409, 688, 707, 1733, 1933
SduI	GDGCHC	2 cut(s)	630, 1972
SexAI	ACCWGGT	1 cut(s)	407
SfaNI	GCATC	8 cut(s)	256, 629, 651, 810, 1171, 1526, 1579, 1783
SfcI	CTRYAG	1 cut(s)	1626
SfoI	GGCGCC	1 cut(s)	521
SinI	GGWCC	3 cut(s)	759, 906, 991
SmiMI	CAYNNNNRTG	3 cut(s)	10, 1009, 1965
SmlI	CTYRAG	2 cut(s)	554, 1016
SmoI	CTYRAG	2 cut(s)	554, 1016
SphI	GCATGC	1 cut(s)	803
SspDI	GGCGCC	1 cut(s)	519
SspI	AATATT	2 cut(s)	1282, 1401
SspMI	CTAG	5 cut(s)	59, 206, 813, 1083, 1665
StyD4I	CCNGG	5 cut(s)	407, 686, 705, 1731, 1931
StyI	CCWWGG	3 cut(s)	109, 980, 1200
TaaI	ACNGT	4 cut(s)	67, 725, 937, 1902
TaiI	ACGT	4 cut(s)	185, 426, 744, 1212
TaqI	TCGA	5 cut(s)	237, 345, 1764, 2144, 2148
TaqII	GACCGA	1 cut(s)	1250
TatI	WGTACW	1 cut(s)	496
TauI	GCSGC	4 cut(s)	354, 477, 1265, 1363
TfiI	GAWTC	6 cut(s)	120, 570, 586, 921, 985, 2141
Tru1I	TTAA	3 cut(s)	492, 1055, 2126
Tru9I	TTAA	3 cut(s)	492, 1055, 2126
TscAI	CASTG	8 cut(s)	70, 439, 630, 635, 705, 787, 942, 2176
TspDTI	ATGAA	4 cut(s)	265, 890, 1164, 1484
TspRI	CASTG	8 cut(s)	70, 439, 630, 635, 705, 787, 942, 2176
Van91I	CCANNNNNTGG	1 cut(s)	1803
VneI	GTGCAC	1 cut(s)	626
VpaK11BI	GGWCC	3 cut(s)	759, 906, 991
XapI	RAATTY	5 cut(s)	862, 953, 1151, 1755, 1948
XceI	RCATGY	4 cut(s)	508, 803, 844, 2083
XmiI	GTMKAC	1 cut(s)	131
XspI	CTAG	5 cut(s)	59, 206, 813, 1083, 1665
ZrmI	AGTACT	1 cut(s)	498
Zsp2I	ATGCAT	2 cut(s)	644, 2085

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.