Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0008964

Physical Location & Seq

Genomic Coordinates Reverse (-)

35717326 .. 35722701

Gene Structure 5376 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1262800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2316 bp

ATGACGTTTTCATGCACGAAAAACTTCTTCCGGAGGTTCTGTATTGATGAATACCAAATGGACACTGTTGCTCAGAGCAGCTTCTTCTCTACTGATCTTCTGCCTTCCCTTGGAGCCAGAATCAACCAGTCTACTAAGCTCAGGAAATACATTATATCGCCATATAATCCTCGTTACAGGGCTTGGGGGATGCTACTTGTTCTTCTAGTCATCTACTCAGCGTGGATTTGCCCATTTGAGTTTGCATTTCTGCCTTACAAGCGGGATGCTCTTTTCGTCATTGACAACATTGTCAACGGCTTCTTTGGCATTGACATCATTCTCACTTTCTTTGTTGCCTATCTCGACAGCCGCTCTTACCTTCTTGTTGACAATCCAAAGCAAATCGCAATGAGGTACTTATCAACCTGGTTTCTTTTCGACGTGTGTTCCACTGCACCATTTCAGTCTATTAGCCTCCTCTTGACAAATCACGGCAGCGAACTTGAGTTTAAAGTACTCAATATGCTCCGCCTCTGGCGCCTCCGACGAGTCAGCTCCCTCTTTGCAAGACTGGAGAAGGACATCCGATTCAACTACTTCTGGATTCGCTGCACGAAGCTCATTTCTGTTACCCTTTTCGCAGTGCACTGCGCAGGATGCTTCAACTATCTGATCGCAGATCGGTATCCTGACCCGAAAAGAACATGGATCGGCGCTGTGTACCCGGATTTCAAACAAGATAGTCTCTGGAACAGATATGTTACTTCAATGTACTGGTCAATCACAACGCTAACCACCACTGGCTATGGAGATCTGCATGCTGAGAACCCTAGAGAGATGCTGTTTGATATTTTCTACATGCTCTTCAACTTGGGATTGACATCTTACCTCATTGGAAACATGACAAATCTTGTAGTTCACTGGACCAGCAGAACCAGAATCTTTAGGGACACAGTGAGAGCTGCATCAGAATTTGCAGCAAGAAACGACTTGCCCCCAACGATTCAAGACCAGATGTTGTCACACATATGCCTCAAGTTTAAGACAGAAGGACTGAAACAGCAAGAGACCTTAAATGGTCTCCCGAAAGCCCTTCGTTCCAGCATTGCCCAACATCTCTTCTTCCCCGTCGTTCAAAGCATCTACCTCTTTCAAGGAGTTTCTTATGATTTCCTCTTTCAATTGGTTTCAGAAATAGATGCAGAGTATTTTCCACCCAAGGAAGATGTAATTCTGCAAAACGAGGCTCCGACCGATCTTTACATACTGGTTTCCGGTGCAGCGGATCTGGTGTCCAATGTTGATGGGCAAGCTCAATTTATACGAAAGGCAACTGCGGGGGATACTTTGGGAGAAATCGGAGTATTATGTCATAGGCCACAGCCCTTCACCGTTCGGACAACCGAACTTTCCCAGATATTACGAATCCGCAGAAGTTCACTCATGACCACCATAGAAGCAAATAAGGACGACGAGCAAATTATCATGAACAACATTTTTCTGAAACTGAAGGAACAAGAAGGGTTGGGCTGTGAATATCCACATACCGAAGGATGCTGTTCTTGCGCTGGATGCAAAGACAACTCACCTCAAGAAACATCAATGGAGGAAGCAAGGAACGACTTGTTTACAGGTTCAGAGGCTACAAAAAAGAGCGAAATAGGCAGATCTGATATTTCAACGAGATGTGCAATGGATGTCAGCATGATGGCTGAGGATGGCCAAACGGCTCTTCACACTGCTGTTCGTCAGGGACATATGGAAATGGTCAAAATTTTGGTTGAAGGAGGAGCAAATGTAAACAAACCAGATGCTAGAGGATGGAAGCCGAAAGATCTAGCACAACAGCAAGGAAACAAGAGCATAACTGACCTTTTACGAATATATGAGAATAGGAGGAGAACAGATGAACATAGAATAGAGTTTATTGAACCGGAAACATCTGAAATAACCAGGAATTGTAAAGGAAATTCCAAAAGACAAGAGGGTGCCCAATTTTCCCACTCTCACCAGAGAAAAGTACCCATTAAGTCCTACCCGAGCAATTCTATCCCTGATGGAGAACGGATGAGATCAATCAACAGGAGAGTAACTATCCACATGCATTTTCAAAATGGAAGTGCATCGGAGAGGCAGCCTGCGAAGTTAATTATCCTACCTGATTCGATGGAAGAGCTACTCAGAGTTGCCGGTGAGAAGTTCGGAGGATACAAACCTACAAAAGTCGTTAATGAAGAAAATGCAGAAATAGATGACATAAGTGTTGTTCGAGATGGTGATCACCTGTATCTTCTTCACAACGACAATAATAATATGAATTCTGATGCGGCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005216 GO:0005242 GO:0005244 GO:0005249 GO:0005261 GO:0005267 GO:0005488 GO:0005515 GO:0005575 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0007623 GO:0008150 GO:0008324 GO:0009314 GO:0009416 GO:0009628 GO:0009642 GO:0009644 GO:0009987 GO:0010118 GO:0015075 GO:0015077 GO:0015079 GO:0015267 GO:0015276 GO:0015318 GO:0015672 GO:0016020 GO:0016021 GO:0022803 GO:0022832 GO:0022834 GO:0022836 GO:0022838 GO:0022839 GO:0022843 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042391 GO:0042802 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0048511 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0065007 GO:0065008 GO:0071804 GO:0071805 GO:0071944 GO:0098655 GO:0098660 GO:0098662 GO:0099094

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ion_trans KHA cNMP_binding

Protein Analysis

772

Amino Acids

88.35

Weight (kDa)

6.65

Isoelectric Point (pI)

46.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ion_trans	PF00520	59 - 306	3.5e-43	Ion transport protein
Ion_trans_2	PF07885	234 - 299	2.2e-12	Ion channel
cNMP_binding	PF00027	397 - 481	1.1e-14	Cyclic nucleotide-binding domain
Ank_2	PF12796	560 - 621	7e-09	Ankyrin repeats (3 copies)
Ank_5	PF13857	566 - 608	3.8e-06	Ankyrin repeats (many copies)
Ank	PF00023	567 - 598	7.3e-09	Ankyrin repeat
Ank_3	PF13606	567 - 595	4.1e-06	Ankyrin repeat
KHA	PF11834	689 - 758	7.3e-27	KHA, dimerisation domain of potassium ion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0008964)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G18290 AT4G18290 AT5G46240
fragaria_vesca	FvH4_3g05650 FvH4_3g05650
malus_domestica	MD05G1284400.v1.1 MD10G1262800.v1.1
prunus_persica	Prupe.4G080000_v2.0.a1 Prupe.4G080000_v2.0.a1
pyrus_communis	pycom05g26360
rosa_chinensis	RchiOBHm_Chr5g0013991
rosa_laevigata	RLG00000032060
rosa_multiflora	Rmu_co8232297.1_g000001 Rmu_co8474133.1_g000001 Rmu_sc0005803.1_g000012
rosa_roxburghii	Rroxscaffold_1G00062720
rosa_rugosa	Rorug05G0013900
rosa_samantha	Rh5AG108400 Rh5BG105200 Rh5CG117000 Rh5DG103900
rosa_wichuraiana	Rw5G009460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	290
Acc16I	TGCGCA	1 cut(s)	634
AccB1I	GGYRCC	2 cut(s)	519, 1970
AccBSI	CCGCTC	1 cut(s)	354
AccI	GTMKAC	1 cut(s)	131
AccIII	TCCGGA	1 cut(s)	30
AciI	CCGC	7 cut(s)	262, 352, 511, 1265, 1319, 1411, 2309
AclWI	GGATC	2 cut(s)	698, 1275
AcoI	YGGCCR	1 cut(s)	1702
AcsI	RAATTY	4 cut(s)	953, 1755, 1951, 2299
AcuI	CTGAAG	1 cut(s)	1511
AcyI	GRCGYC	1 cut(s)	520
AfaI	GTAC	5 cut(s)	398, 498, 704, 755, 2004
AfiI	CCNNNNNNNGG	1 cut(s)	110
AflIII	ACRYGT	1 cut(s)	423
AjiI	CACGTC	1 cut(s)	424
AjnI	CCWGG	2 cut(s)	407, 1934
AjuI	GAANNNNNNNTTGG	2 cut(s)	1698, 1730
AluBI	AGCT	7 cut(s)	81, 139, 537, 601, 944, 1295, 2158
AluI	AGCT	7 cut(s)	81, 139, 537, 601, 944, 1295, 2158
Alw21I	GWGCWC	1 cut(s)	630
Alw26I	GTCTC	3 cut(s)	731, 1043, 1067
Alw44I	GTGCAC	1 cut(s)	626
AlwI	GGATC	2 cut(s)	698, 1275
Ama87I	CYCGRG	1 cut(s)	2020
Aor13HI	TCCGGA	1 cut(s)	30
AoxI	GGCC	2 cut(s)	1358, 1702
ApaLI	GTGCAC	1 cut(s)	626
ApeKI	GCWGC	7 cut(s)	78, 477, 591, 944, 959, 1262, 2116
ApoI	RAATTY	4 cut(s)	953, 1755, 1951, 2299
Asp700I	GAANNNNTTC	1 cut(s)	23
AspLEI	GCGC	4 cut(s)	522, 635, 698, 1550
AspS9I	GGNCC	1 cut(s)	906
AsuC2I	CCSGG	1 cut(s)	707
AsuHPI	GGTGA	6 cut(s)	1363, 1560, 1982, 2186, 2255, 2270
AvaI	CYCGRG	1 cut(s)	2020
AvaII	GGWCC	1 cut(s)	906
BaeGI	GKGCMC	2 cut(s)	630, 1975
BalI	TGGCCA	1 cut(s)	1704
BanI	GGYRCC	2 cut(s)	519, 1970
Bbv12I	GWGCWC	1 cut(s)	630
BbvCI	CCTCAGC	1 cut(s)	1695
BbvI	GCAGC	7 cut(s)	90, 489, 578, 931, 971, 1274, 2128
BccI	CCATC	7 cut(s)	1280, 1684, 1694, 1797, 2033, 2143, 2249
BceAI	ACGGC	3 cut(s)	313, 490, 1725
BciT130I	CCWGG	2 cut(s)	409, 1936
BciVI	GTATCC	3 cut(s)	678, 1318, 2183
BclI	TGATCA	1 cut(s)	2260
BcnI	CCSGG	1 cut(s)	707
BcoDI	GTCTC	3 cut(s)	731, 1043, 1067
BfaI	CTAG	4 cut(s)	206, 813, 1797, 1820
BfoI	RGCGCY	2 cut(s)	523, 699
BfuI	GTATCC	3 cut(s)	678, 1318, 2183
BglII	AGATCT	3 cut(s)	793, 1649, 1816
BisI	GCNGC	9 cut(s)	79, 352, 478, 592, 945, 960, 1263, 2117, 2310
BlsI	GCNGC	9 cut(s)	80, 353, 479, 593, 946, 961, 1264, 2118, 2311
BmcAI	AGTACT	1 cut(s)	498
Bme1390I	CCNGG	3 cut(s)	409, 707, 1936
Bme18I	GGWCC	1 cut(s)	906
BmeT110I	CYCGRG	1 cut(s)	2020
BmgBI	CACGTC	1 cut(s)	424
BmgT120I	GGNCC	1 cut(s)	906
BmiI	GGNNCC	4 cut(s)	115, 521, 1230, 1972
BmrFI	CCNGG	3 cut(s)	409, 707, 1936
BpmI	CTGGAG	1 cut(s)	575
Bpu10I	CCTNAGC	2 cut(s)	140, 1695
BpuEI	CTTGAG	3 cut(s)	506, 1001, 1557
BpuMI	CCSGG	1 cut(s)	707
BsaBI	GATNNNNATC	3 cut(s)	666, 862, 2259
BsaHI	GRCGYC	1 cut(s)	520
BsaI	GGTCTC	2 cut(s)	1043, 1067
BsaJI	CCNNGG	2 cut(s)	109, 1200
BsaWI	WCCGGW	3 cut(s)	30, 1256, 1915
BsaXI	ACNNNNNCTCC	4 cut(s)	25, 55, 1764, 1794
Bsc4I	CCNNNNNNNGG	1 cut(s)	110
Bse118I	RCCGGY	1 cut(s)	2171
Bse1I	ACTGG	6 cut(s)	127, 558, 761, 787, 908, 1254
Bse3DI	GCAATG	3 cut(s)	396, 1086, 1680
Bse8I	GATNNNNATC	3 cut(s)	666, 862, 2259
BseAI	TCCGGA	1 cut(s)	30
BseBI	CCWGG	2 cut(s)	409, 1936
BseDI	CCNNGG	2 cut(s)	109, 1200
BseJI	GATNNNNATC	3 cut(s)	666, 862, 2259
BseLI	CCNNNNNNNGG	1 cut(s)	110
BseMI	GCAATG	3 cut(s)	396, 1086, 1680
BseMII	CTCAG	6 cut(s)	86, 154, 231, 795, 1686, 2176
BseNI	ACTGG	6 cut(s)	127, 558, 761, 787, 908, 1254
BseRI	GAGGAG	3 cut(s)	449, 1785, 1894
BseSI	GKGCMC	2 cut(s)	630, 1975
BseXI	GCAGC	7 cut(s)	90, 489, 578, 931, 971, 1274, 2128
BsgI	GTGCAG	3 cut(s)	420, 577, 1281
Bsh1285I	CGRYCG	1 cut(s)	1236
BshFI	GGCC	2 cut(s)	1360, 1704
BshNI	GGYRCC	2 cut(s)	519, 1970
BsiEI	CGRYCG	1 cut(s)	1236
BsiHKAI	GWGCWC	1 cut(s)	630
BsiHKCI	CYCGRG	1 cut(s)	2020
BsiSI	CCGG	5 cut(s)	31, 707, 1257, 1916, 2172
BslFI	GGGAC	2 cut(s)	944, 1749
BslI	CCNNNNNNNGG	1 cut(s)	110
BsmAI	GTCTC	3 cut(s)	731, 1043, 1067
BsmFI	GGGAC	2 cut(s)	944, 1749
BsnI	GGCC	2 cut(s)	1360, 1704
Bso31I	GGTCTC	2 cut(s)	1043, 1067
BsoBI	CYCGRG	1 cut(s)	2020
Bsp1286I	GDGCHC	2 cut(s)	630, 1975
Bsp13I	TCCGGA	1 cut(s)	30
BspACI	CCGC	7 cut(s)	262, 352, 511, 1265, 1319, 1411, 2309
BspANI	GGCC	2 cut(s)	1360, 1704
BspCNI	CTCAG	6 cut(s)	85, 153, 230, 796, 1687, 2175
BspEI	TCCGGA	1 cut(s)	30
BspHI	TCATGA	2 cut(s)	1425, 1467
BspLI	GGNNCC	4 cut(s)	115, 521, 1230, 1972
BspPI	GGATC	2 cut(s)	698, 1275
BspQI	GCTCTTC	3 cut(s)	851, 1719, 2148
BspT107I	GGYRCC	2 cut(s)	519, 1970
BspTNI	GGTCTC	2 cut(s)	1043, 1067
BsrBI	CCGCTC	1 cut(s)	354
BsrDI	GCAATG	3 cut(s)	396, 1086, 1680
BsrFI	RCCGGY	1 cut(s)	2171
BsrI	ACTGG	6 cut(s)	127, 558, 761, 787, 908, 1254
BssAI	RCCGGY	1 cut(s)	2171
BssECI	CCNNGG	2 cut(s)	109, 1200
BssNI	GRCGYC	1 cut(s)	520
BssT1I	CCWWGG	2 cut(s)	109, 1200
Bst2UI	CCWGG	2 cut(s)	409, 1936
Bst4CI	ACNGT	3 cut(s)	67, 937, 1375
Bst6I	CTCTTC	4 cut(s)	851, 1106, 1719, 2148
BstACI	GRCGYC	1 cut(s)	520
BstC8I	GCNNGC	3 cut(s)	801, 1293, 2121
BstDEI	CTNAG	7 cut(s)	72, 135, 140, 217, 804, 1695, 2162
BstH2I	RGCGCY	2 cut(s)	523, 699
BstHHI	GCGC	4 cut(s)	522, 635, 698, 1550
BstMAI	GTCTC	3 cut(s)	731, 1043, 1067
BstMCI	CGRYCG	1 cut(s)	1236
BstMWI	GCNNNNNNNGC	7 cut(s)	259, 306, 519, 639, 1545, 1554, 1644
BstNI	CCWGG	2 cut(s)	409, 1936
BstNSI	RCATGY	3 cut(s)	803, 844, 2086
BstSCI	CCNGG	3 cut(s)	407, 705, 1934
BstSLI	GKGCMC	2 cut(s)	630, 1975
BstV1I	GCAGC	7 cut(s)	90, 489, 578, 931, 971, 1274, 2128
BstX2I	RGATCY	4 cut(s)	793, 1267, 1649, 1816
BstYI	RGATCY	4 cut(s)	793, 1267, 1649, 1816
BsuI	GTATCC	3 cut(s)	678, 1318, 2183
BsuRI	GGCC	2 cut(s)	1360, 1704
BtrI	CACGTC	1 cut(s)	424
BtsI	GCAGTG	4 cut(s)	432, 628, 630, 1719
BtsIMutI	CAGTG	8 cut(s)	63, 432, 628, 630, 780, 901, 942, 1719
Cac8I	GCNNGC	3 cut(s)	801, 1293, 2121
CciI	TCATGA	2 cut(s)	1425, 1467
CfoI	GCGC	4 cut(s)	522, 635, 698, 1550
Cfr10I	RCCGGY	1 cut(s)	2171
Cfr13I	GGNCC	1 cut(s)	906
CsiI	ACCWGGT	1 cut(s)	407
Csp6I	GTAC	5 cut(s)	397, 497, 703, 754, 2003
CviAII	CATG	9 cut(s)	12, 687, 800, 841, 883, 1426, 1468, 1687, 2083
CviQI	GTAC	5 cut(s)	397, 497, 703, 754, 2003
DdeI	CTNAG	7 cut(s)	72, 135, 140, 217, 804, 1695, 2162
DinI	GGCGCC	1 cut(s)	521
DraI	TTTAAA	1 cut(s)	493
DrdI	GACNNNNNNGTC	1 cut(s)	290
DseDI	GACNNNNNNGTC	1 cut(s)	290
EaeI	YGGCCR	1 cut(s)	1702
Eam1104I	CTCTTC	4 cut(s)	851, 1106, 1719, 2148
EarI	CTCTTC	4 cut(s)	851, 1106, 1719, 2148
EciI	GGCGGA	1 cut(s)	500
Eco130I	CCWWGG	2 cut(s)	109, 1200
Eco31I	GGTCTC	2 cut(s)	1043, 1067
Eco47I	GGWCC	1 cut(s)	906
Eco57I	CTGAAG	1 cut(s)	1511
Eco88I	CYCGRG	1 cut(s)	2020
EcoRI	GAATTC	1 cut(s)	2299
EcoRII	CCWGG	2 cut(s)	407, 1934
EcoT14I	CCWWGG	2 cut(s)	109, 1200
EcoT22I	ATGCAT	1 cut(s)	2088
EgeI	GGCGCC	1 cut(s)	521
EheI	GGCGCC	1 cut(s)	521
ErhI	CCWWGG	2 cut(s)	109, 1200
FaeI	CATG	9 cut(s)	15, 690, 803, 844, 886, 1429, 1471, 1690, 2086
FaqI	GGGAC	2 cut(s)	944, 1749
FatI	CATG	9 cut(s)	11, 686, 799, 840, 882, 1425, 1467, 1686, 2082
FauI	CCCGC	2 cut(s)	255, 1312
FauNDI	CATATG	2 cut(s)	1010, 1740
FbaI	TGATCA	1 cut(s)	2260
FblI	GTMKAC	1 cut(s)	131
Fnu4HI	GCNGC	9 cut(s)	79, 352, 478, 592, 945, 960, 1263, 2117, 2310
Fsp4HI	GCNGC	9 cut(s)	79, 352, 478, 592, 945, 960, 1263, 2117, 2310
FspBI	CTAG	4 cut(s)	206, 813, 1797, 1820
FspI	TGCGCA	1 cut(s)	634
GlaI	GCGC	4 cut(s)	521, 634, 697, 1549
GluI	GCNGC	9 cut(s)	79, 352, 478, 592, 945, 960, 1263, 2117, 2310
GsuI	CTGGAG	1 cut(s)	575
HaeII	RGCGCY	2 cut(s)	523, 699
HaeIII	GGCC	2 cut(s)	1360, 1704
HapII	CCGG	5 cut(s)	31, 707, 1257, 1916, 2172
HhaI	GCGC	4 cut(s)	522, 635, 698, 1550
Hin1I	GRCGYC	1 cut(s)	520
Hin1II	CATG	9 cut(s)	15, 690, 803, 844, 886, 1429, 1471, 1690, 2086
Hin6I	GCGC	4 cut(s)	520, 633, 696, 1548
HinP1I	GCGC	4 cut(s)	520, 633, 696, 1548
HincII	GTYRAC	2 cut(s)	295, 370
HindII	GTYRAC	2 cut(s)	295, 370
HinfI	GANTC	8 cut(s)	120, 531, 570, 586, 921, 985, 1407, 2144
HpaII	CCGG	5 cut(s)	31, 707, 1257, 1916, 2172
HphI	GGTGA	6 cut(s)	1363, 1560, 1982, 2186, 2255, 2270
Hpy166II	GTNNAC	9 cut(s)	132, 295, 370, 628, 703, 901, 1421, 1611, 1783
Hpy8I	GTNNAC	9 cut(s)	132, 295, 370, 628, 703, 901, 1421, 1611, 1783
Hpy99I	CGWCG	4 cut(s)	425, 531, 1115, 1457
HpyCH4III	ACNGT	3 cut(s)	67, 937, 1375
HpyCH4IV	ACGT	2 cut(s)	5, 423
HpyF10VI	GCNNNNNNNGC	7 cut(s)	259, 306, 519, 639, 1545, 1554, 1644
HpyF3I	CTNAG	7 cut(s)	72, 135, 140, 217, 804, 1695, 2162
HpySE526I	ACGT	2 cut(s)	5, 423
Hsp92I	GRCGYC	1 cut(s)	520
Hsp92II	CATG	9 cut(s)	15, 690, 803, 844, 886, 1429, 1471, 1690, 2086
HspAI	GCGC	4 cut(s)	520, 633, 696, 1548
KasI	GGCGCC	1 cut(s)	519
Kpn2I	TCCGGA	1 cut(s)	30
Ksp22I	TGATCA	1 cut(s)	2260
LguI	GCTCTTC	3 cut(s)	851, 1719, 2148
LmnI	GCTCC	5 cut(s)	113, 513, 542, 1234, 1772
Lsp1109I	GCAGC	7 cut(s)	90, 489, 578, 931, 971, 1274, 2128
MabI	ACCWGGT	1 cut(s)	407
MaeI	CTAG	4 cut(s)	206, 813, 1797, 1820
MaeII	ACGT	2 cut(s)	5, 423
MaeIII	GTNAC	5 cut(s)	173, 610, 742, 1002, 2071
MbiI	CCGCTC	1 cut(s)	354
MfeI	CAATTG	1 cut(s)	1163
MflI	RGATCY	4 cut(s)	793, 1267, 1649, 1816
MhlI	GDGCHC	2 cut(s)	630, 1975
MlsI	TGGCCA	1 cut(s)	1704
MluNI	TGGCCA	1 cut(s)	1704
Mly113I	GGCGCC	1 cut(s)	520
MlyI	GAGTC	1 cut(s)	540
MmeI	TCCRAC	2 cut(s)	550, 1256
Mox20I	TGGCCA	1 cut(s)	1704
Mph1103I	ATGCAT	1 cut(s)	2088
MroI	TCCGGA	1 cut(s)	30
MroXI	GAANNNNTTC	1 cut(s)	23
MscI	TGGCCA	1 cut(s)	1704
MseI	TTAA	7 cut(s)	492, 1023, 1055, 2010, 2129, 2211, 2314
MslI	CAYNNNNRTG	1 cut(s)	1009
Msp20I	TGGCCA	1 cut(s)	1704
MspA1I	CMGCKG	1 cut(s)	1265
MspI	CCGG	5 cut(s)	31, 707, 1257, 1916, 2172
MspR9I	CCNGG	3 cut(s)	409, 707, 1936
MunI	CAATTG	1 cut(s)	1163
MvaI	CCWGG	2 cut(s)	409, 1936
MwoI	GCNNNNNNNGC	7 cut(s)	259, 306, 519, 639, 1545, 1554, 1644
NarI	GGCGCC	1 cut(s)	520
NciI	CCSGG	1 cut(s)	707
NdeI	CATATG	2 cut(s)	1010, 1740
NlaIII	CATG	9 cut(s)	15, 690, 803, 844, 886, 1429, 1471, 1690, 2086
NlaIV	GGNNCC	4 cut(s)	115, 521, 1230, 1972
NmuCI	GTSAC	1 cut(s)	1002
NsbI	TGCGCA	1 cut(s)	634
NsiI	ATGCAT	1 cut(s)	2088
NspI	RCATGY	3 cut(s)	803, 844, 2086
PaeI	GCATGC	1 cut(s)	803
PagI	TCATGA	2 cut(s)	1425, 1467
PciSI	GCTCTTC	3 cut(s)	851, 1719, 2148
PdmI	GAANNNNTTC	1 cut(s)	23
PfeI	GAWTC	7 cut(s)	120, 570, 586, 921, 985, 1407, 2144
PkrI	GCNGC	9 cut(s)	80, 353, 479, 593, 946, 961, 1264, 2118, 2311
PleI	GAGTC	1 cut(s)	539
PluTI	GGCGCC	1 cut(s)	523
PpsI	GAGTC	1 cut(s)	539
Psp6I	CCWGG	2 cut(s)	407, 1934
PspGI	CCWGG	2 cut(s)	407, 1934
PspN4I	GGNNCC	4 cut(s)	115, 521, 1230, 1972
PspPI	GGNCC	1 cut(s)	906
PsuI	RGATCY	4 cut(s)	793, 1267, 1649, 1816
RsaI	GTAC	5 cut(s)	398, 498, 704, 755, 2004
RsaNI	GTAC	5 cut(s)	397, 497, 703, 754, 2003
RseI	CAYNNNNRTG	1 cut(s)	1009
SapI	GCTCTTC	3 cut(s)	851, 1719, 2148
SaqAI	TTAA	7 cut(s)	492, 1023, 1055, 2010, 2129, 2211, 2314
SatI	GCNGC	9 cut(s)	79, 352, 478, 592, 945, 960, 1263, 2117, 2310
Sau96I	GGNCC	1 cut(s)	906
ScaI	AGTACT	1 cut(s)	498
SchI	GAGTC	1 cut(s)	540
ScrFI	CCNGG	3 cut(s)	409, 707, 1936
SduI	GDGCHC	2 cut(s)	630, 1975
SexAI	ACCWGGT	1 cut(s)	407
SfoI	GGCGCC	1 cut(s)	521
SinI	GGWCC	1 cut(s)	906
SmiMI	CAYNNNNRTG	1 cut(s)	1009
SmlI	CTYRAG	3 cut(s)	485, 1016, 1572
SmoI	CTYRAG	3 cut(s)	485, 1016, 1572
SphI	GCATGC	1 cut(s)	803
SsiI	CCGC	7 cut(s)	262, 352, 511, 1265, 1319, 1411, 2309
SspDI	GGCGCC	1 cut(s)	519
SspMI	CTAG	4 cut(s)	206, 813, 1797, 1820
StyD4I	CCNGG	3 cut(s)	407, 705, 1934
StyI	CCWWGG	2 cut(s)	109, 1200
TaaI	ACNGT	3 cut(s)	67, 937, 1375
TaiI	ACGT	2 cut(s)	8, 426
TaqI	TCGA	4 cut(s)	345, 420, 2147, 2251
TaqII	GACCGA	1 cut(s)	1250
TatI	WGTACW	2 cut(s)	496, 753
TauI	GCSGC	2 cut(s)	354, 2312
TfiI	GAWTC	7 cut(s)	120, 570, 586, 921, 985, 1407, 2144
Tru1I	TTAA	7 cut(s)	492, 1023, 1055, 2010, 2129, 2211, 2314
Tru9I	TTAA	7 cut(s)	492, 1023, 1055, 2010, 2129, 2211, 2314
TscAI	CASTG	8 cut(s)	70, 439, 630, 635, 787, 908, 942, 1726
TseFI	GTSAC	1 cut(s)	1002
TseI	GCWGC	7 cut(s)	78, 477, 591, 944, 959, 1262, 2116
Tsp45I	GTSAC	1 cut(s)	1002
TspDTI	ATGAA	5 cut(s)	63, 1484, 1905, 2229, 2312
TspGWI	ACGGA	1 cut(s)	2062
TspRI	CASTG	8 cut(s)	70, 439, 630, 635, 787, 908, 942, 1726
VneI	GTGCAC	1 cut(s)	626
VpaK11BI	GGWCC	1 cut(s)	906
XapI	RAATTY	4 cut(s)	953, 1755, 1951, 2299
XceI	RCATGY	3 cut(s)	803, 844, 2086
XmiI	GTMKAC	1 cut(s)	131
XmnI	GAANNNNTTC	1 cut(s)	23
XspI	CTAG	4 cut(s)	206, 813, 1797, 1820
ZrmI	AGTACT	1 cut(s)	498
Zsp2I	ATGCAT	1 cut(s)	2088

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.