Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0008964

Physical Location & Seq

Genomic Coordinates Forward (+)

10528656 .. 10532191

Gene Structure 3536 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000032060

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2451 bp

ATGTCAGTTTCCGGCACAAAGAATTTCTTCAGGAGGTTTTGTATTGATGAATACCAAATAGATAGTGTTGCTCAGAGCAGTTTCTTCTCTAGTGATCTATTGCCATCTCTTGGAGCCAGAATCAACCAATCAACCAAGCTCCGAAAATACATTATATCGCCTTACAATCCTCGTTATAGGGCTTGGGAGATGTTACTCATTGTTCTCGTCATTTACTCCGCCTGGATTTGCCCATTCGAGTTTGCATTCTTGACTTACAAGCAAGATGCTCTTTTTATCATCGACAACATTGTCAACGGTTTCTTTGCCATTGACATCTTCCTCACTTTCTTTGTTGCATATCTCGACAGCCAGTCTTATCTTCTTGTTGACAATCCCAAGCAAATTGCAACGAGGTACATATCAACCTGGTTTATTTTCGATGCCTGTTCCACTGCCCCATTCCAGCCTATTATTAGCCTCTTGTTTACAAATCATGGCAGCGAACTTGGCTTTAAAGTACTGAACATGCTCCGGCTCTGGCGCCTCAGACGAGTGAGCTCCCTGTTTGCAAGACTTGAGAAGGACATTCGATTCAATTACTTCTGGACTCGGTGCACAAAGCTCATTTCTGTGACCCTTTTCGCAGTACATTGTGCTGGATGCTTTTACTATCTGATAGCAGACAGATACCCCGATTCAAAACAAACATGGATCGGTGCAGTGTACCCAAATTTCAAAGAGGATAGTCTTTGGAACAGATACATCACTGCGATGTACTGGTCTATCACAACACTCACAACCACTGGCTATGGAGACTTGCATGCTGAGAACCCAAGGGAGATGCTGTTCGATATTTTCTACATGCTGTTCAACTTGGGATTGACAGCTTACCTCATCGGAAACATGACAAACCTTGTGGTTCATTCGACCAGCAGAACCAGAAACTTCAGGGACACAGTGCGAGCTGCTTCAGAATTTGCAGCAAGAAATGACTTGCCGCCAAGAATACAGGACCAAATGTTGTCACACATATGCCTCAAGTTCAGGACAGAAGGGTTGAAGCAGCAAGAGACCTTGAATGATCTCCCCAAAGCGCTTCGTTCAAGCATTGCTCAGCATCTCTTCTTCCCCATTATTCAAAAAGTCGATATCTTCCAAGGAGTTTCATATGACTTCCTTTTCCAATTGGTAAAGCATCACATTGTCTTCTTTTTTAAAGTCAGTATCGTACCTTCAGTTCACATTATCATTCAAATTTCTCACAAGCAGCTAAAACCATTTATTGTTTTCACAAAGGTATCAGAAATAGAGGCCGAGTACTTTCCACCCAAAGAAGAAGTCATTCTGCAAAATGAGGCCCCAACGGATCTTTACATACTGGTCTCAGGTGCAGTAGTTTTAATATCCAATATTGATGGGCAAGAGCAAGTTGTAGGAAAGGCAAATTCTGGGGATACTTTGGGCGAAATTGGAGTATTATGTCAGAGGCCACAGCCTTACACAGTTCGGACAACTGAACTTTCCCAAATATTACGACTCCGCAGAAGTTCACTGATGACCACTATACAAACAAATAAGGAAGACGAGCAGATTATCATGAACAACATTTTTATGAAACTGAAAGGGGAAGATGGCTCAGGCTTTGAATATCCACATACAAATCCAGGCTTAATGCTTGACAATTGTCCTGGAGGAGGACAGAGAGGAGGATGCAAAGATAGCTCACATGAAGATGCAGCAATGCATGAAGCAAGGAACAATTGCGTTACAGCTTCGCAGGCTAGGAAGAAGAGTGAAATAGGCAAAGCTCAAATGACAGGTGAGAATGGCCAAACTGAAATTCAAGCTGCTGTTTGCCAGGGCCATCTGGAAAAGGTAAAAATTGTGGTTGAAGGAGGAGCAGATGTCAACAAACAGGAAGCTAGAGGATGGATGCCAAAAGGTCTAGCACAACAGCAAGGAGACAAGGACATACATGACCTCTTATTAAGTTACGAAAATAGAAGAGAAATGGATGAACATAGAATAGAGTTCATTGAGCCAGAAACATCTGGAAGCACCAGCAATTGTGAAGGAAATTGTACAACACAGGAAGACCACCAACATATTCATTCTCACTTGAGAAAGGTAGCCATGAAGTCATATCAGTGCCCGTCTAGCCCTGCAACTGACAGAGAACAAAGGATCAGATCATACAGCAAGAGAGTAACTATCCACATGCATTCTAAAAGTGAAAGTGTGCCGGAAAGGCAGCTTGCAAAATTAATAATTCTACCTGATTCCATAGACGAGCTGCTCAGAGTTGCTAGTGAGAAGTTCGGATGCTACAGACCTACAAAAGTCGTGAATGCAGAGAATGCAGAAATAGAAGACATGAGCGTCGTTAGGGATGGTGATCATCTGTTTCTTCTTCATAATGATTGTCAAAATTTGTACTCTGATGTGACCCAACGTTGTTCGAAAATGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005216 GO:0005242 GO:0005244 GO:0005249 GO:0005261 GO:0005267 GO:0005488 GO:0005515 GO:0005575 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0007623 GO:0008150 GO:0008324 GO:0009314 GO:0009416 GO:0009628 GO:0009642 GO:0009644 GO:0009987 GO:0010118 GO:0015075 GO:0015077 GO:0015079 GO:0015267 GO:0015276 GO:0015318 GO:0015672 GO:0016020 GO:0016021 GO:0022803 GO:0022832 GO:0022834 GO:0022836 GO:0022838 GO:0022839 GO:0022843 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042391 GO:0042802 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0048511 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0065007 GO:0065008 GO:0071804 GO:0071805 GO:0071944 GO:0098655 GO:0098660 GO:0098662 GO:0099094

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ank_2 Ion_trans KHA cNMP_binding

Protein Analysis

817

Amino Acids

93.39

Weight (kDa)

6.79

Isoelectric Point (pI)

47.38

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ion_trans	PF00520	59 - 307	7.9e-44	Ion transport protein
Ion_trans_2	PF07885	220 - 300	1.2e-12	Ion channel
cNMP_binding	PF00027	434 - 519	2.7e-16	Cyclic nucleotide-binding domain
KHA	PF11834	728 - 797	7.7e-25	KHA, dimerisation domain of potassium ion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008964)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G18290 AT4G18290 AT5G46240
fragaria_vesca	FvH4_3g05650 FvH4_3g05650
malus_domestica	MD05G1284400.v1.1 MD10G1262800.v1.1
prunus_persica	Prupe.4G080000_v2.0.a1 Prupe.4G080000_v2.0.a1
pyrus_communis	pycom05g26360
rosa_chinensis	RchiOBHm_Chr5g0013991
rosa_laevigata	RLG00000032060
rosa_multiflora	Rmu_co8232297.1_g000001 Rmu_co8474133.1_g000001 Rmu_sc0005803.1_g000012
rosa_roxburghii	Rroxscaffold_1G00062720
rosa_rugosa	Rorug05G0013900
rosa_samantha	Rh5AG108400 Rh5BG105200 Rh5CG117000 Rh5DG103900
rosa_wichuraiana	Rw5G009460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	290, 2360
AccB1I	GGYRCC	1 cut(s)	522
AccB7I	CCANNNNNTGG	1 cut(s)	110
AciI	CCGC	3 cut(s)	219, 980, 1522
AclI	AACGTT	1 cut(s)	2434
AclWI	GGATC	3 cut(s)	701, 1356, 2174
AcoI	YGGCCR	1 cut(s)	1810
AcsI	RAATTY	7 cut(s)	22, 712, 956, 1236, 1426, 1821, 2410
AcuI	CTGAAG	4 cut(s)	13, 913, 936, 1200
AcyI	GRCGYC	1 cut(s)	523
AfaI	GTAC	9 cut(s)	398, 501, 630, 707, 758, 1212, 1301, 2065, 2417
AfeI	AGCGCT	1 cut(s)	1077
AfiI	CCNNNNNNNGG	2 cut(s)	110, 1676
AhdI	GACNNNNNGTC	1 cut(s)	352
AjnI	CCWGG	5 cut(s)	221, 407, 1645, 1669, 1839
AjuI	GAANNNNNNNTTGG	2 cut(s)	1806, 1838
AloI	GAACNNNNNNTCC	2 cut(s)	812, 844
Alw21I	GWGCWC	2 cut(s)	542, 599
Alw26I	GTCTC	4 cut(s)	789, 1046, 1369, 1938
Alw44I	GTGCAC	1 cut(s)	595
AlwI	GGATC	3 cut(s)	701, 1356, 2174
Aor51HI	AGCGCT	1 cut(s)	1077
AoxI	GGCC	5 cut(s)	1293, 1338, 1469, 1810, 1843
ApaLI	GTGCAC	1 cut(s)	595
ApeKI	GCWGC	9 cut(s)	480, 947, 962, 1045, 1249, 1718, 1829, 2233, 2275
ApoI	RAATTY	7 cut(s)	22, 712, 956, 1236, 1426, 1821, 2410
ArsI	GACNNNNNNTTYG	2 cut(s)	1305, 1337
AseI	ATTAAT	1 cut(s)	2246
Asp700I	GAANNNNTTC	2 cut(s)	26, 1323
AspLEI	GCGC	2 cut(s)	525, 1078
AspS9I	GGNCC	3 cut(s)	994, 1339, 1843
AsuHPI	GGTGA	2 cut(s)	1814, 2387
AsuII	TTCGAA	1 cut(s)	2441
AvaII	GGWCC	1 cut(s)	994
BaeGI	GKGCMC	2 cut(s)	599, 2135
BaeI	ACNNNNGTAYC	2 cut(s)	1263, 1296
BalI	TGGCCA	1 cut(s)	1812
BanI	GGYRCC	1 cut(s)	522
BanII	GRGCYC	1 cut(s)	542
BarI	GAAGNNNNNNTAC	2 cut(s)	1198, 1230
BbsI	GAAGAC	4 cut(s)	1180, 1569, 2082, 2358
Bbv12I	GWGCWC	2 cut(s)	542, 599
BbvI	GCAGC	9 cut(s)	492, 934, 974, 1057, 1261, 1730, 1816, 2245, 2262
BccI	CCATC	6 cut(s)	112, 1391, 1607, 1854, 1905, 2366
BciT130I	CCWGG	5 cut(s)	223, 409, 1647, 1671, 1841
BciVI	GTATCC	1 cut(s)	1429
BclI	TGATCA	1 cut(s)	2377
BcoDI	GTCTC	4 cut(s)	789, 1046, 1369, 1938
BfaI	CTAG	6 cut(s)	90, 1764, 1905, 1928, 2139, 2289
BfmI	CTRYAG	1 cut(s)	2308
BfoI	RGCGCY	2 cut(s)	526, 1079
BfuI	GTATCC	1 cut(s)	1429
BglI	GCCNNNNNGGC	1 cut(s)	2230
BlpI	GCTNAGC	1 cut(s)	1095
BmcAI	AGTACT	2 cut(s)	501, 1301
Bme1390I	CCNGG	5 cut(s)	223, 409, 1647, 1671, 1841
Bme18I	GGWCC	1 cut(s)	994
BmeRI	GACNNNNNGTC	1 cut(s)	352
BmgT120I	GGNCC	3 cut(s)	994, 1339, 1843
BmiI	GGNNCC	3 cut(s)	115, 524, 1341
BmrFI	CCNGG	5 cut(s)	223, 409, 1647, 1671, 1841
BoxI	GACNNNNGTC	1 cut(s)	1665
BpiI	GAAGAC	4 cut(s)	1180, 1569, 2082, 2358
BpmI	CTGGAG	1 cut(s)	1692
Bpu10I	CCTNAGC	1 cut(s)	1618
Bpu1102I	GCTNAGC	1 cut(s)	1095
Bpu14I	TTCGAA	1 cut(s)	2441
BpuEI	CTTGAG	3 cut(s)	578, 1004, 2122
BsaBI	GATNNNNATC	1 cut(s)	2376
BsaHI	GRCGYC	1 cut(s)	523
BsaI	GGTCTC	2 cut(s)	1046, 1369
BsaJI	CCNNGG	3 cut(s)	815, 1138, 1840
BsaXI	ACNNNNNCTCC	6 cut(s)	25, 55, 812, 842, 1872, 1902
Bsc4I	CCNNNNNNNGG	2 cut(s)	110, 1676
Bse1I	ACTGG	4 cut(s)	352, 764, 790, 1365
Bse3DI	GCAATG	2 cut(s)	1089, 1728
Bse8I	GATNNNNATC	1 cut(s)	2376
BseBI	CCWGG	5 cut(s)	223, 409, 1647, 1671, 1841
BseDI	CCNNGG	3 cut(s)	815, 1138, 1840
BseGI	GGATG	7 cut(s)	647, 1697, 1916, 1920, 2002, 2309, 2377
BseJI	GATNNNNATC	1 cut(s)	2376
BseLI	CCNNNNNNNGG	2 cut(s)	110, 1676
BseMI	GCAATG	2 cut(s)	1089, 1728
BseMII	CTCAG	7 cut(s)	86, 541, 798, 1109, 1380, 1632, 2293
BseNI	ACTGG	4 cut(s)	352, 764, 790, 1365
BseRI	GAGGAG	3 cut(s)	1689, 1701, 1893
BseSI	GKGCMC	2 cut(s)	599, 2135
BseXI	GCAGC	9 cut(s)	492, 934, 974, 1057, 1261, 1730, 1816, 2245, 2262
BsgI	GTGCAG	2 cut(s)	720, 1392
BshFI	GGCC	5 cut(s)	1295, 1340, 1471, 1812, 1845
BshNI	GGYRCC	1 cut(s)	522
BsiHKAI	GWGCWC	2 cut(s)	542, 599
BsiSI	CCGG	3 cut(s)	12, 514, 2225
BslFI	GGGAC	1 cut(s)	947
BslI	CCNNNNNNNGG	2 cut(s)	110, 1676
BsmAI	GTCTC	4 cut(s)	789, 1046, 1369, 1938
BsmFI	GGGAC	1 cut(s)	947
BsmI	GAATGC	4 cut(s)	245, 2203, 2335, 2344
BsnI	GGCC	5 cut(s)	1295, 1340, 1471, 1812, 1845
Bso31I	GGTCTC	2 cut(s)	1046, 1369
Bsp119I	TTCGAA	1 cut(s)	2441
Bsp1286I	GDGCHC	3 cut(s)	542, 599, 2135
Bsp1407I	TGTACA	1 cut(s)	2063
Bsp143I	GATC	7 cut(s)	94, 693, 1063, 1348, 2166, 2171, 2377
Bsp1720I	GCTNAGC	1 cut(s)	1095
BspACI	CCGC	3 cut(s)	219, 980, 1522
BspANI	GGCC	5 cut(s)	1295, 1340, 1471, 1812, 1845
BspCNI	CTCAG	7 cut(s)	85, 540, 799, 1108, 1379, 1631, 2292
BspHI	TCATGA	1 cut(s)	1578
BspLI	GGNNCC	3 cut(s)	115, 524, 1341
BspPI	GGATC	3 cut(s)	701, 1356, 2174
BspT104I	TTCGAA	1 cut(s)	2441
BspT107I	GGYRCC	1 cut(s)	522
BspTNI	GGTCTC	2 cut(s)	1046, 1369
BsrDI	GCAATG	2 cut(s)	1089, 1728
BsrGI	TGTACA	1 cut(s)	2063
BsrI	ACTGG	4 cut(s)	352, 764, 790, 1365
BssECI	CCNNGG	3 cut(s)	815, 1138, 1840
BssMI	GATC	7 cut(s)	94, 693, 1063, 1348, 2166, 2171, 2377
BssNI	GRCGYC	1 cut(s)	523
BssT1I	CCWWGG	2 cut(s)	815, 1138
Bst2UI	CCWGG	5 cut(s)	223, 409, 1647, 1671, 1841
Bst4CI	ACNGT	3 cut(s)	299, 940, 1486
Bst6I	CTCTTC	3 cut(s)	1109, 1766, 1981
BstACI	GRCGYC	1 cut(s)	523
BstAPI	GCANNNNNTGC	1 cut(s)	2339
BstAUI	TGTACA	1 cut(s)	2063
BstBI	TTCGAA	1 cut(s)	2441
BstC8I	GCNNGC	4 cut(s)	804, 945, 1761, 2238
BstDEI	CTNAG	7 cut(s)	72, 527, 807, 1095, 1366, 1618, 2279
BstENI	CCTNNNNNAGG	1 cut(s)	1674
BstF5I	GGATG	7 cut(s)	647, 1697, 1916, 1920, 2002, 2309, 2377
BstH2I	RGCGCY	2 cut(s)	526, 1079
BstHHI	GCGC	2 cut(s)	525, 1078
BstKTI	GATC	7 cut(s)	97, 696, 1066, 1351, 2169, 2174, 2380
BstMAI	GTCTC	4 cut(s)	789, 1046, 1369, 1938
BstMBI	GATC	7 cut(s)	94, 693, 1063, 1348, 2166, 2171, 2377
BstMWI	GCNNNNNNNGC	6 cut(s)	489, 1701, 1760, 2139, 2230, 2339
BstNI	CCWGG	5 cut(s)	223, 409, 1647, 1671, 1841
BstNSI	RCATGY	4 cut(s)	511, 806, 847, 2203
BstPAI	GACNNNNGTC	1 cut(s)	1665
BstSCI	CCNGG	5 cut(s)	221, 407, 1645, 1669, 1839
BstSFI	CTRYAG	1 cut(s)	2308
BstSLI	GKGCMC	2 cut(s)	599, 2135
BstV1I	GCAGC	9 cut(s)	492, 934, 974, 1057, 1261, 1730, 1816, 2245, 2262
BstV2I	GAAGAC	4 cut(s)	1180, 1569, 2082, 2358
BstX2I	RGATCY	1 cut(s)	1348
BstYI	RGATCY	1 cut(s)	1348
BsuI	GTATCC	1 cut(s)	1429
BsuRI	GGCC	5 cut(s)	1295, 1340, 1471, 1812, 1845
BtgZI	GCGATG	1 cut(s)	767
BtsCI	GGATG	7 cut(s)	647, 1697, 1916, 1920, 2002, 2309, 2377
BtsI	GCAGTG	3 cut(s)	432, 708, 747
BtsIMutI	CAGTG	7 cut(s)	432, 708, 747, 783, 945, 1532, 2135
Cac8I	GCNNGC	4 cut(s)	804, 945, 1761, 2238
CciI	TCATGA	1 cut(s)	1578
CfoI	GCGC	2 cut(s)	525, 1078
Cfr13I	GGNCC	3 cut(s)	994, 1339, 1843
CseI	GACGC	1 cut(s)	2350
CsiI	ACCWGGT	1 cut(s)	407
Csp6I	GTAC	9 cut(s)	397, 500, 629, 706, 757, 1211, 1300, 2064, 2416
CspCI	CAANNNNNGTGG	2 cut(s)	879, 914
CviQI	GTAC	9 cut(s)	397, 500, 629, 706, 757, 1211, 1300, 2064, 2416
DdeI	CTNAG	7 cut(s)	72, 527, 807, 1095, 1366, 1618, 2279
DinI	GGCGCC	1 cut(s)	524
DpnI	GATC	7 cut(s)	96, 695, 1065, 1350, 2168, 2173, 2379
DpnII	GATC	7 cut(s)	94, 693, 1063, 1348, 2166, 2171, 2377
DraI	TTTAAA	2 cut(s)	496, 1198
DrdI	GACNNNNNNGTC	2 cut(s)	290, 2360
DriI	GACNNNNNGTC	1 cut(s)	352
DseDI	GACNNNNNNGTC	2 cut(s)	290, 2360
EaeI	YGGCCR	1 cut(s)	1810
Eam1104I	CTCTTC	3 cut(s)	1109, 1766, 1981
Eam1105I	GACNNNNNGTC	1 cut(s)	352
EarI	CTCTTC	3 cut(s)	1109, 1766, 1981
EciI	GGCGGA	1 cut(s)	208
Ecl136II	GAGCTC	1 cut(s)	540
Eco130I	CCWWGG	2 cut(s)	815, 1138
Eco24I	GRGCYC	1 cut(s)	542
Eco31I	GGTCTC	2 cut(s)	1046, 1369
Eco32I	GATATC	1 cut(s)	1132
Eco47I	GGWCC	1 cut(s)	994
Eco47III	AGCGCT	1 cut(s)	1077
Eco53kI	GAGCTC	1 cut(s)	540
Eco57I	CTGAAG	4 cut(s)	13, 913, 936, 1200
EcoICRI	GAGCTC	1 cut(s)	540
EcoNI	CCTNNNNNAGG	1 cut(s)	1674
EcoO109I	RGGNCCY	1 cut(s)	1339
EcoRII	CCWGG	5 cut(s)	221, 407, 1645, 1669, 1839
EcoRV	GATATC	1 cut(s)	1132
EcoT14I	CCWWGG	2 cut(s)	815, 1138
EcoT22I	ATGCAT	2 cut(s)	1728, 2205
EcoT38I	GRGCYC	1 cut(s)	542
EgeI	GGCGCC	1 cut(s)	524
EheI	GGCGCC	1 cut(s)	524
ErhI	CCWWGG	2 cut(s)	815, 1138
FalI	AAGNNNNNCTT	4 cut(s)	11, 43, 255, 287
FaqI	GGGAC	1 cut(s)	947
FauNDI	CATATG	2 cut(s)	1013, 1150
FbaI	TGATCA	1 cut(s)	2377
FokI	GGATG	7 cut(s)	654, 1704, 1923, 1927, 2009, 2316, 2384
FriOI	GRGCYC	1 cut(s)	542
FspBI	CTAG	6 cut(s)	90, 1764, 1905, 1928, 2139, 2289
GlaI	GCGC	2 cut(s)	524, 1077
GsuI	CTGGAG	1 cut(s)	1692
HaeII	RGCGCY	2 cut(s)	526, 1079
HaeIII	GGCC	5 cut(s)	1295, 1340, 1471, 1812, 1845
HapII	CCGG	3 cut(s)	12, 514, 2225
HgaI	GACGC	1 cut(s)	2350
HhaI	GCGC	2 cut(s)	525, 1078
Hin1I	GRCGYC	1 cut(s)	523
Hin6I	GCGC	2 cut(s)	523, 1076
HinP1I	GCGC	2 cut(s)	523, 1076
HincII	GTYRAC	3 cut(s)	295, 370, 1891
HindII	GTYRAC	3 cut(s)	295, 370, 1891
HinfI	GANTC	6 cut(s)	120, 573, 589, 677, 1518, 2261
HpaII	CCGG	3 cut(s)	12, 514, 2225
HphI	GGTGA	2 cut(s)	1814, 2387
Hpy166II	GTNNAC	8 cut(s)	295, 370, 468, 597, 706, 1222, 1532, 1891
Hpy188III	TCNNGA	9 cut(s)	31, 250, 344, 586, 1027, 1579, 1850, 2034, 2326
Hpy8I	GTNNAC	8 cut(s)	295, 370, 468, 597, 706, 1222, 1532, 1891
Hpy99I	CGWCG	1 cut(s)	2366
HpyAV	CCTTC	5 cut(s)	556, 1028, 1224, 1868, 2048
HpyCH4III	ACNGT	3 cut(s)	299, 940, 1486
HpyCH4IV	ACGT	1 cut(s)	2434
HpyF10VI	GCNNNNNNNGC	6 cut(s)	489, 1701, 1760, 2139, 2230, 2339
HpyF3I	CTNAG	7 cut(s)	72, 527, 807, 1095, 1366, 1618, 2279
HpySE526I	ACGT	1 cut(s)	2434
Hsp92I	GRCGYC	1 cut(s)	523
HspAI	GCGC	2 cut(s)	523, 1076
KasI	GGCGCC	1 cut(s)	522
Ksp22I	TGATCA	1 cut(s)	2377
Kzo9I	GATC	7 cut(s)	94, 693, 1063, 1348, 2166, 2171, 2377
LmnI	GCTCC	5 cut(s)	113, 144, 516, 545, 1880
Lsp1109I	GCAGC	9 cut(s)	492, 934, 974, 1057, 1261, 1730, 1816, 2245, 2262
MabI	ACCWGGT	1 cut(s)	407
MaeI	CTAG	6 cut(s)	90, 1764, 1905, 1928, 2139, 2289
MaeII	ACGT	1 cut(s)	2434
MaeIII	GTNAC	7 cut(s)	192, 613, 1005, 1747, 1973, 2188, 2425
MalI	GATC	7 cut(s)	96, 695, 1065, 1350, 2168, 2173, 2379
MboI	GATC	7 cut(s)	94, 693, 1063, 1348, 2166, 2171, 2377
MfeI	CAATTG	4 cut(s)	1166, 1663, 1741, 2047
MflI	RGATCY	1 cut(s)	1348
MhlI	GDGCHC	3 cut(s)	542, 599, 2135
MlsI	TGGCCA	1 cut(s)	1812
MluNI	TGGCCA	1 cut(s)	1812
Mly113I	GGCGCC	1 cut(s)	523
MlyI	GAGTC	2 cut(s)	583, 1512
Mox20I	TGGCCA	1 cut(s)	1812
Mph1103I	ATGCAT	2 cut(s)	1728, 2205
MroXI	GAANNNNTTC	2 cut(s)	26, 1323
MscI	TGGCCA	1 cut(s)	1812
MseI	TTAA	6 cut(s)	495, 1197, 1382, 1652, 1970, 2246
MslI	CAYNNNNRTG	6 cut(s)	611, 752, 1012, 1592, 1713, 2128
Msp20I	TGGCCA	1 cut(s)	1812
MspI	CCGG	3 cut(s)	12, 514, 2225
MspR9I	CCNGG	5 cut(s)	223, 409, 1647, 1671, 1841
MunI	CAATTG	4 cut(s)	1166, 1663, 1741, 2047
Mva1269I	GAATGC	4 cut(s)	245, 2203, 2335, 2344
MvaI	CCWGG	5 cut(s)	223, 409, 1647, 1671, 1841
MwoI	GCNNNNNNNGC	6 cut(s)	489, 1701, 1760, 2139, 2230, 2339
NarI	GGCGCC	1 cut(s)	523
NdeI	CATATG	2 cut(s)	1013, 1150
NdeII	GATC	7 cut(s)	94, 693, 1063, 1348, 2166, 2171, 2377
NlaIV	GGNNCC	3 cut(s)	115, 524, 1341
NmeAIII	GCCGAG	1 cut(s)	1321
NmuCI	GTSAC	3 cut(s)	613, 1005, 2425
NsiI	ATGCAT	2 cut(s)	1728, 2205
NspI	RCATGY	4 cut(s)	511, 806, 847, 2203
NspV	TTCGAA	1 cut(s)	2441
PaeI	GCATGC	1 cut(s)	806
PagI	TCATGA	1 cut(s)	1578
PctI	GAATGC	4 cut(s)	245, 2203, 2335, 2344
PdmI	GAANNNNTTC	2 cut(s)	26, 1323
PfeI	GAWTC	4 cut(s)	120, 573, 677, 2261
PflMI	CCANNNNNTGG	1 cut(s)	110
PfoI	TCCNGGA	1 cut(s)	1669
PleI	GAGTC	2 cut(s)	583, 1512
PluTI	GGCGCC	1 cut(s)	526
PpsI	GAGTC	2 cut(s)	583, 1512
PshAI	GACNNNNGTC	1 cut(s)	1665
PshBI	ATTAAT	1 cut(s)	2246
Psp124BI	GAGCTC	1 cut(s)	542
Psp1406I	AACGTT	1 cut(s)	2434
Psp6I	CCWGG	5 cut(s)	221, 407, 1645, 1669, 1839
PspGI	CCWGG	5 cut(s)	221, 407, 1645, 1669, 1839
PspN4I	GGNNCC	3 cut(s)	115, 524, 1341
PspPI	GGNCC	3 cut(s)	994, 1339, 1843
PsrI	GAACNNNNNNTAC	2 cut(s)	1203, 1235
PsuI	RGATCY	1 cut(s)	1348
RsaI	GTAC	9 cut(s)	398, 501, 630, 707, 758, 1212, 1301, 2065, 2417
RsaNI	GTAC	9 cut(s)	397, 500, 629, 706, 757, 1211, 1300, 2064, 2416
RseI	CAYNNNNRTG	6 cut(s)	611, 752, 1012, 1592, 1713, 2128
SacI	GAGCTC	1 cut(s)	542
SaqAI	TTAA	6 cut(s)	495, 1197, 1382, 1652, 1970, 2246
Sau3AI	GATC	7 cut(s)	94, 693, 1063, 1348, 2166, 2171, 2377
Sau96I	GGNCC	3 cut(s)	994, 1339, 1843
ScaI	AGTACT	2 cut(s)	501, 1301
SchI	GAGTC	2 cut(s)	583, 1512
ScrFI	CCNGG	5 cut(s)	223, 409, 1647, 1671, 1841
SduI	GDGCHC	3 cut(s)	542, 599, 2135
SexAI	ACCWGGT	1 cut(s)	407
SfcI	CTRYAG	1 cut(s)	2308
SfoI	GGCGCC	1 cut(s)	524
SfuI	TTCGAA	1 cut(s)	2441
SinI	GGWCC	1 cut(s)	994
SmiMI	CAYNNNNRTG	6 cut(s)	611, 752, 1012, 1592, 1713, 2128
SmlI	CTYRAG	3 cut(s)	557, 1019, 2101
SmoI	CTYRAG	3 cut(s)	557, 1019, 2101
SphI	GCATGC	1 cut(s)	806
SsiI	CCGC	3 cut(s)	219, 980, 1522
SspDI	GGCGCC	1 cut(s)	522
SspI	AATATT	2 cut(s)	1393, 1512
SspMI	CTAG	6 cut(s)	90, 1764, 1905, 1928, 2139, 2289
SstI	GAGCTC	1 cut(s)	542
StyD4I	CCNGG	5 cut(s)	221, 407, 1645, 1669, 1839
StyI	CCWWGG	2 cut(s)	815, 1138
TaaI	ACNGT	3 cut(s)	299, 940, 1486
TaiI	ACGT	1 cut(s)	2437
TaqI	TCGA	9 cut(s)	237, 282, 345, 420, 571, 831, 908, 1128, 2441
TatI	WGTACW	6 cut(s)	499, 628, 756, 1299, 2063, 2415
TauI	GCSGC	1 cut(s)	982
TfiI	GAWTC	4 cut(s)	120, 573, 677, 2261
Tru1I	TTAA	6 cut(s)	495, 1197, 1382, 1652, 1970, 2246
Tru9I	TTAA	6 cut(s)	495, 1197, 1382, 1652, 1970, 2246
TscAI	CASTG	7 cut(s)	439, 708, 754, 790, 945, 1539, 2135
TseFI	GTSAC	3 cut(s)	613, 1005, 2425
TseI	GCWGC	9 cut(s)	480, 947, 962, 1045, 1249, 1718, 1829, 2233, 2275
Tsp45I	GTSAC	3 cut(s)	613, 1005, 2425
TspGWI	ACGGA	1 cut(s)	1361
TspRI	CASTG	7 cut(s)	439, 708, 754, 790, 945, 1539, 2135
Van91I	CCANNNNNTGG	1 cut(s)	110
VneI	GTGCAC	1 cut(s)	595
VpaK11BI	GGWCC	1 cut(s)	994
VspI	ATTAAT	1 cut(s)	2246
XagI	CCTNNNNNAGG	1 cut(s)	1674
XapI	RAATTY	7 cut(s)	22, 712, 956, 1236, 1426, 1821, 2410
XceI	RCATGY	4 cut(s)	511, 806, 847, 2203
XmnI	GAANNNNTTC	2 cut(s)	26, 1323
XspI	CTAG	6 cut(s)	90, 1764, 1905, 1928, 2139, 2289
ZrmI	AGTACT	2 cut(s)	501, 1301
Zsp2I	ATGCAT	2 cut(s)	1728, 2205

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.