FvH4_3g11400

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. The coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0012024
Physical Location & Seq
Reverse (-)
6720895 .. 6723391
2497 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g11400.t2

Sequence Viewer

Length: 882 bp
ATGGCAGCACTAGCAGCAGGACCGGACCACCTCTTCAACCTCCGCAACAACTTCTACCTTGGCGCCTACCAGGCCGCCATCAACAGCAGCGACCTCCCCAACCTCTCGCCGGACGAAGCCGTCGAGCGCGACTGCCTCGTTTTCCGATCCTACATAGCCCTCGGCAGCTACCAGCTCGTCATCAACGAGATCGACTCCTCCGCCGCCACTCCTCTCCAGGCCGTCAAGCTCCTCGCTCTCTACCTCTCCGGCCCCGATTACAAGGAATCGGTGATCTCGAATCTTAAGGAGTTGCTGGCGGATCCGGCGATCGGGAACAACCCGATTCTGAGGATGGTCGCCGGGACTGTGTTTATGCACGAGCAGGACTACAATGAAGCTCTCAAGTACACGCATGCCGGCGGCAATTTGGAACTGGATGCGCTGAATGTTCAAATATTCATCAAGATGCATAGGGCTGAATTTGCTGAAAGAAAGCTGAAGGAAATGCAAAAGATTGATGAGGACCACACCCTAACTCAACTTGCAACAGCATGGCAAAATCTGGCAGTGGGTGGTTCTAAGATACAGGAGGCATATCTCATCTTCCAAGATTTCTCTGAGAAGTCTCAGGTTACAACCTTGATTTTGAATGGAAAGGCTGTTTGCTGCATACACATGGGAAACTTTGATGAAGCTGAAACACTGCTTCTTGAAGCACTGAACAAGGATGCAAAGGATCCAGAAACTCTAGCCAACCTGGTAGTATGCTGTCTTCACCTTGGAAAATCACCTTCTCGTTTTCTCAGCCAATTAAGACTTGCACATCCAGAGCATGTCCTCGTCAAACGTGCCGCAGCTGCAGAAGAGAGCTTTGACAGAGCTGTTCAATCAGTTGCTTGA

Protein Analysis

294

Amino Acids

32.3

Weight (kDa)

5.2

Isoelectric Point (pI)

36.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Coatomer_E PF04733 8 - 237 7.9e-107 Coatomer epsilon subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012024)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G30630 AT2G34840 AT2G34840
fragaria_vesca FvH4_3g11400 FvH4_3g11400
malus_domestica MD05G1263700.v1.1 MD10G1241400.v1.1
prunus_persica Prupe.4G101800_v2.0.a1
pyrus_communis pycom05g24770 pycom10g20240
rosa_chinensis RchiOBHm_Chr5g0018341
rosa_laevigata RLG00000032353
rosa_multiflora Rmu_sc0004464.1_g000001
rosa_roxburghii Rroxscaffold_1G00059400
rosa_rugosa Rorug05G0041600
rosa_samantha Rh5BG133500 Rh5CG145300 Rh5DG134900
rosa_wichuraiana Rw5G011780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 119
AccB1I GGYRCC 1 cut(s) 62
AccII CGCG 1 cut(s) 129
AciI CCGC 7 cut(s) 43, 75, 201, 204, 299, 402, 834
AclWI GGATC 5 cut(s) 141, 296, 309, 713, 726
AcsI RAATTY 1 cut(s) 461
AcuI CTGAAG 1 cut(s) 500
AcyI GRCGYC 1 cut(s) 63
AfaI GTAC 1 cut(s) 389
AfiI CCNNNNNNNGG 2 cut(s) 109, 311
AflII CTTAAG 1 cut(s) 284
AgsI TTSAA 5 cut(s) 37, 434, 631, 695, 869
AjnI CCWGG 3 cut(s) 69, 216, 738
AluBI AGCT 9 cut(s) 168, 175, 229, 380, 478, 677, 839, 852, 863
AluI AGCT 9 cut(s) 168, 175, 229, 380, 478, 677, 839, 852, 863
Alw26I GTCTC 1 cut(s) 612
AlwI GGATC 5 cut(s) 141, 296, 309, 713, 726
AoxI GGCC 3 cut(s) 72, 219, 250
ApeKI GCWGC 7 cut(s) 5, 14, 87, 165, 648, 836, 839
ApoI RAATTY 1 cut(s) 461
AspLEI GCGC 3 cut(s) 65, 129, 424
AspS9I GGNCC 4 cut(s) 20, 25, 251, 505
AsuC2I CCSGG 1 cut(s) 343
AsuHPI GGTGA 3 cut(s) 283, 749, 762
AvaII GGWCC 3 cut(s) 20, 25, 505
BamHI GGATCC 2 cut(s) 301, 718
BanI GGYRCC 1 cut(s) 62
BarI GAAGNNNNNNTAC 2 cut(s) 738, 770
BauI CACGAG 1 cut(s) 359
BbsI GAAGAC 1 cut(s) 746
BbvI GCAGC 7 cut(s) 17, 26, 99, 177, 635, 826, 848
BccI CCATC 2 cut(s) 86, 328
BceAI ACGGC 2 cut(s) 104, 206
BciT130I CCWGG 3 cut(s) 71, 218, 740
BcnI CCSGG 1 cut(s) 343
BcoDI GTCTC 1 cut(s) 612
BfaI CTAG 2 cut(s) 11, 731
BfmI CTRYAG 1 cut(s) 840
BfoI RGCGCY 1 cut(s) 66
BfrI CTTAAG 1 cut(s) 284
BglI GCCNNNNNGGC 1 cut(s) 71
Bme1390I CCNGG 4 cut(s) 71, 218, 343, 740
Bme18I GGWCC 3 cut(s) 20, 25, 505
BmgT120I GGNCC 4 cut(s) 20, 25, 251, 505
BmiI GGNNCC 4 cut(s) 64, 253, 303, 720
BmrFI CCNGG 4 cut(s) 71, 218, 343, 740
BmsI GCATC 3 cut(s) 409, 438, 700
BpiI GAAGAC 1 cut(s) 746
BplI GAGNNNNNCTC 2 cut(s) 179, 211
BpmI CTGGAG 1 cut(s) 200
BpuEI CTTGAG 1 cut(s) 368
BpuMI CCSGG 1 cut(s) 343
BsaHI GRCGYC 1 cut(s) 63
BsaJI CCNNGG 3 cut(s) 58, 160, 760
BsaWI WCCGGW 1 cut(s) 22
Bsc4I CCNNNNNNNGG 2 cut(s) 109, 311
Bse118I RCCGGY 1 cut(s) 398
Bse1I ACTGG 1 cut(s) 420
BseBI CCWGG 3 cut(s) 71, 218, 740
BseDI CCNNGG 3 cut(s) 58, 160, 760
BseGI GGATG 4 cut(s) 339, 424, 715, 805
BseLI CCNNNNNNNGG 2 cut(s) 109, 311
BseMII CTCAG 4 cut(s) 320, 591, 623, 799
BseNI ACTGG 1 cut(s) 420
BseRI GAGGAG 3 cut(s) 187, 201, 221
BseXI GCAGC 7 cut(s) 17, 26, 99, 177, 635, 826, 848
Bsh1236I CGCG 1 cut(s) 129
Bsh1285I CGRYCG 1 cut(s) 312
BshFI GGCC 3 cut(s) 74, 221, 252
BshNI GGYRCC 1 cut(s) 62
BsiEI CGRYCG 1 cut(s) 312
BsiSI CCGG 6 cut(s) 23, 110, 249, 305, 342, 399
BslFI GGGAC 1 cut(s) 358
BslI CCNNNNNNNGG 2 cut(s) 109, 311
BsmAI GTCTC 1 cut(s) 612
BsmFI GGGAC 1 cut(s) 358
BsnI GGCC 3 cut(s) 74, 221, 252
Bsp143I GATC 6 cut(s) 146, 189, 273, 301, 309, 718
BspACI CCGC 7 cut(s) 43, 75, 201, 204, 299, 402, 834
BspANI GGCC 3 cut(s) 74, 221, 252
BspCNI CTCAG 4 cut(s) 321, 592, 622, 798
BspFNI CGCG 1 cut(s) 129
BspLI GGNNCC 4 cut(s) 64, 253, 303, 720
BspMAI CTGCAG 1 cut(s) 844
BspPI GGATC 5 cut(s) 141, 296, 309, 713, 726
BspT107I GGYRCC 1 cut(s) 62
BspTI CTTAAG 1 cut(s) 284
BsrFI RCCGGY 1 cut(s) 398
BsrI ACTGG 1 cut(s) 420
BssAI RCCGGY 1 cut(s) 398
BssECI CCNNGG 3 cut(s) 58, 160, 760
BssMI GATC 6 cut(s) 146, 189, 273, 301, 309, 718
BssNI GRCGYC 1 cut(s) 63
BssSI CACGAG 1 cut(s) 359
BssT1I CCWWGG 2 cut(s) 58, 760
Bst2BI CACGAG 1 cut(s) 359
Bst2UI CCWGG 3 cut(s) 71, 218, 740
Bst4CI ACNGT 1 cut(s) 349
Bst6I CTCTTC 2 cut(s) 38, 840
BstACI GRCGYC 1 cut(s) 63
BstAFI CTTAAG 1 cut(s) 284
BstC8I GCNNGC 3 cut(s) 297, 396, 400
BstDEI CTNAG 5 cut(s) 329, 561, 600, 609, 785
BstF5I GGATG 4 cut(s) 339, 424, 715, 805
BstFNI CGCG 1 cut(s) 129
BstH2I RGCGCY 1 cut(s) 66
BstHHI GCGC 3 cut(s) 65, 129, 424
BstKTI GATC 6 cut(s) 149, 192, 276, 304, 312, 721
BstMAI GTCTC 1 cut(s) 612
BstMBI GATC 6 cut(s) 146, 189, 273, 301, 309, 718
BstMCI CGRYCG 1 cut(s) 312
BstMWI GCNNNNNNNGC 6 cut(s) 11, 14, 71, 305, 464, 839
BstNI CCWGG 3 cut(s) 71, 218, 740
BstNSI RCATGY 2 cut(s) 398, 818
BstSCI CCNGG 4 cut(s) 69, 216, 341, 738
BstSFI CTRYAG 1 cut(s) 840
BstUI CGCG 1 cut(s) 129
BstV1I GCAGC 7 cut(s) 17, 26, 99, 177, 635, 826, 848
BstV2I GAAGAC 1 cut(s) 746
BstX2I RGATCY 2 cut(s) 301, 718
BstYI RGATCY 2 cut(s) 301, 718
BsuRI GGCC 3 cut(s) 74, 221, 252
BtsCI GGATG 4 cut(s) 339, 424, 715, 805
BtsI GCAGTG 2 cut(s) 555, 683
BtsIMutI CAGTG 3 cut(s) 555, 683, 698
Cac8I GCNNGC 3 cut(s) 297, 396, 400
CfoI GCGC 3 cut(s) 65, 129, 424
Cfr10I RCCGGY 1 cut(s) 398
Cfr13I GGNCC 4 cut(s) 20, 25, 251, 505
CsiI ACCWGGT 1 cut(s) 738
Csp6I GTAC 1 cut(s) 388
CviAII CATG 4 cut(s) 395, 534, 658, 815
CviQI GTAC 1 cut(s) 388
DdeI CTNAG 5 cut(s) 329, 561, 600, 609, 785
DinI GGCGCC 1 cut(s) 64
DpnI GATC 6 cut(s) 148, 191, 275, 303, 311, 720
DpnII GATC 6 cut(s) 146, 189, 273, 301, 309, 718
DrdI GACNNNNNNGTC 1 cut(s) 119
DseDI GACNNNNNNGTC 1 cut(s) 119
Eam1104I CTCTTC 2 cut(s) 38, 840
EarI CTCTTC 2 cut(s) 38, 840
EciI GGCGGA 2 cut(s) 190, 314
Eco130I CCWWGG 2 cut(s) 58, 760
Eco47I GGWCC 3 cut(s) 20, 25, 505
Eco57I CTGAAG 1 cut(s) 500
EcoRII CCWGG 3 cut(s) 69, 216, 738
EcoT14I CCWWGG 2 cut(s) 58, 760
EcoT22I ATGCAT 1 cut(s) 453
EgeI GGCGCC 1 cut(s) 64
EheI GGCGCC 1 cut(s) 64
ErhI CCWWGG 2 cut(s) 58, 760
FaeI CATG 4 cut(s) 398, 537, 661, 818
FaqI GGGAC 1 cut(s) 358
FatI CATG 4 cut(s) 394, 533, 657, 814
FokI GGATG 4 cut(s) 346, 431, 722, 792
FspBI CTAG 2 cut(s) 11, 731
GlaI GCGC 3 cut(s) 64, 128, 423
GsuI CTGGAG 1 cut(s) 200
HaeII RGCGCY 1 cut(s) 66
HaeIII GGCC 3 cut(s) 74, 221, 252
HapII CCGG 6 cut(s) 23, 110, 249, 305, 342, 399
HhaI GCGC 3 cut(s) 65, 129, 424
Hin1I GRCGYC 1 cut(s) 63
Hin1II CATG 4 cut(s) 398, 537, 661, 818
Hin6I GCGC 3 cut(s) 63, 127, 422
HinP1I GCGC 3 cut(s) 63, 127, 422
HinfI GANTC 4 cut(s) 194, 266, 280, 325
HpaII CCGG 6 cut(s) 23, 110, 249, 305, 342, 399
HphI GGTGA 3 cut(s) 283, 749, 762
Hpy166II GTNNAC 1 cut(s) 390
Hpy188I TCNGA 3 cut(s) 146, 330, 601
Hpy188III TCNNGA 6 cut(s) 277, 313, 445, 692, 722, 809
Hpy8I GTNNAC 1 cut(s) 390
Hpy99I CGWCG 1 cut(s) 125
HpyAV CCTTC 2 cut(s) 475, 783
HpyCH4III ACNGT 1 cut(s) 349
HpyCH4IV ACGT 1 cut(s) 829
HpyCH4V TGCA 8 cut(s) 358, 451, 490, 527, 651, 713, 803, 842
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 14, 71, 305, 464, 839
HpyF3I CTNAG 5 cut(s) 329, 561, 600, 609, 785
HpySE526I ACGT 1 cut(s) 829
Hsp92I GRCGYC 1 cut(s) 63
Hsp92II CATG 4 cut(s) 398, 537, 661, 818
HspAI GCGC 3 cut(s) 63, 127, 422
KasI GGCGCC 1 cut(s) 62
KroI GCCGGC 1 cut(s) 398
KroNI GCCGGC 1 cut(s) 400
Kzo9I GATC 6 cut(s) 146, 189, 273, 301, 309, 718
LmnI GCTCC 1 cut(s) 234
Lsp1109I GCAGC 7 cut(s) 17, 26, 99, 177, 635, 826, 848
LweI GCATC 3 cut(s) 409, 438, 700
MabI ACCWGGT 1 cut(s) 738
MaeI CTAG 2 cut(s) 11, 731
MaeII ACGT 1 cut(s) 829
MaeIII GTNAC 1 cut(s) 613
MalI GATC 6 cut(s) 148, 191, 275, 303, 311, 720
MboI GATC 6 cut(s) 146, 189, 273, 301, 309, 718
MboII GAAGA 4 cut(s) 25, 577, 746, 857
MflI RGATCY 2 cut(s) 301, 718
MluCI AATT 3 cut(s) 406, 461, 791
Mly113I GGCGCC 1 cut(s) 63
MlyI GAGTC 1 cut(s) 188
Mph1103I ATGCAT 1 cut(s) 453
MroNI GCCGGC 1 cut(s) 398
MseI TTAA 2 cut(s) 285, 794
MslI CAYNNNNRTG 2 cut(s) 446, 656
MspA1I CMGCKG 1 cut(s) 839
MspCI CTTAAG 1 cut(s) 284
MspI CCGG 6 cut(s) 23, 110, 249, 305, 342, 399
MspR9I CCNGG 4 cut(s) 71, 218, 343, 740
MvaI CCWGG 3 cut(s) 71, 218, 740
MvnI CGCG 1 cut(s) 129
MwoI GCNNNNNNNGC 6 cut(s) 11, 14, 71, 305, 464, 839
NaeI GCCGGC 1 cut(s) 400
NarI GGCGCC 1 cut(s) 63
NciI CCSGG 1 cut(s) 343
NdeII GATC 6 cut(s) 146, 189, 273, 301, 309, 718
NgoMIV GCCGGC 1 cut(s) 398
NlaIII CATG 4 cut(s) 398, 537, 661, 818
NlaIV GGNNCC 4 cut(s) 64, 253, 303, 720
NmeAIII GCCGAG 1 cut(s) 141
NsiI ATGCAT 1 cut(s) 453
NspI RCATGY 2 cut(s) 398, 818
PaeI GCATGC 1 cut(s) 398
PcsI WCGNNNNNNNCGW 3 cut(s) 120, 183, 275
PdiI GCCGGC 1 cut(s) 400
PfeI GAWTC 3 cut(s) 266, 280, 325
Ple19I CGATCG 1 cut(s) 312
PleI GAGTC 1 cut(s) 188
PluTI GGCGCC 1 cut(s) 66
PpsI GAGTC 1 cut(s) 188
Psp6I CCWGG 3 cut(s) 69, 216, 738
PspGI CCWGG 3 cut(s) 69, 216, 738
PspN4I GGNNCC 4 cut(s) 64, 253, 303, 720
PspPI GGNCC 4 cut(s) 20, 25, 251, 505
PstI CTGCAG 1 cut(s) 844
PsuI RGATCY 2 cut(s) 301, 718
PvuI CGATCG 1 cut(s) 312
PvuII CAGCTG 1 cut(s) 839
RsaI GTAC 1 cut(s) 389
RsaNI GTAC 1 cut(s) 388
RseI CAYNNNNRTG 2 cut(s) 446, 656
SaqAI TTAA 2 cut(s) 285, 794
Sau3AI GATC 6 cut(s) 146, 189, 273, 301, 309, 718
Sau96I GGNCC 4 cut(s) 20, 25, 251, 505
SchI GAGTC 1 cut(s) 188
ScrFI CCNGG 4 cut(s) 71, 218, 343, 740
SexAI ACCWGGT 1 cut(s) 738
SfaNI GCATC 3 cut(s) 409, 438, 700
SfcI CTRYAG 1 cut(s) 840
SfoI GGCGCC 1 cut(s) 64
SinI GGWCC 3 cut(s) 20, 25, 505
SmiMI CAYNNNNRTG 2 cut(s) 446, 656
SmlI CTYRAG 2 cut(s) 284, 383
SmoI CTYRAG 2 cut(s) 284, 383
SphI GCATGC 1 cut(s) 398
Sse9I AATT 3 cut(s) 406, 461, 791
SsiI CCGC 7 cut(s) 43, 75, 201, 204, 299, 402, 834
SspDI GGCGCC 1 cut(s) 62
SspI AATATT 1 cut(s) 438
SspMI CTAG 2 cut(s) 11, 731
StyD4I CCNGG 4 cut(s) 69, 216, 341, 738
StyI CCWWGG 2 cut(s) 58, 760
TaaI ACNGT 1 cut(s) 349
TaiI ACGT 1 cut(s) 832
TaqI TCGA 3 cut(s) 123, 192, 278
TasI AATT 3 cut(s) 406, 461, 791
TatI WGTACW 1 cut(s) 387
TauI GCSGC 4 cut(s) 77, 206, 405, 836
TfiI GAWTC 3 cut(s) 266, 280, 325
Tru1I TTAA 2 cut(s) 285, 794
Tru9I TTAA 2 cut(s) 285, 794
TscAI CASTG 3 cut(s) 555, 690, 705
TseI GCWGC 7 cut(s) 5, 14, 87, 165, 648, 836, 839
TspDTI ATGAA 3 cut(s) 390, 430, 687
TspRI CASTG 3 cut(s) 555, 690, 705
Vha464I CTTAAG 1 cut(s) 284
VpaK11BI GGWCC 3 cut(s) 20, 25, 505
XapI RAATTY 1 cut(s) 461
XceI RCATGY 2 cut(s) 398, 818
XspI CTAG 2 cut(s) 11, 731
Zsp2I ATGCAT 1 cut(s) 453
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.