MD05G1263700.v1.1

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. The coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0012024
Physical Location & Seq
Forward (+)
39901772 .. 39904472
2701 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1263700.v1.1.491

Sequence Viewer

Length: 882 bp
ATGGCAGCAGTGGCAGCAGGACCGGACCACCTCTTTAACCTCCGCAACAACTTCTACTTGGGCGCCTACCAGGCCGCCATTAACAACAGCGACCTCCCCAACCTCTCCCCCGACGACGCCGTCGAGCGCGACTGCCTCGTCTTCCGCTCTTACATCGCCCTCGGAAGCTACCAGCTCGTCATCAACGAGATCGACGCCTCCGCCGCCACTCCTCTCCAGGCCGTCAAGCTCCTCGCCCTCTACCTGTCTGGCCCCGATTTCAAGGAATCGGCGATCTCGAATTTGAAGGAGTTGTTGGCGGATCCGGCCGTCGGAAACAACCCTACCCTGAGGCTGATTGCTGGGACGGTTTTCATGCATGAGCAGGACTACAATGAAGCTCTCAAATACACCAATTCCGGAGGCACTATGGAACTGAATGCATTGAATATCCAGATATTCCTTAAAATGCATAGGTCAGATTACGCGGAGAGACAGCTGCGGCTCATGCAACAGGTTGACGAGGACCACACGCTAACTCAACTTGCAATGGCATGGTTAAATCTGGCAGTGGGTGGTTCCAAGATACAGGAGGCATATCTCATCTTCCAAGATTTCTCTGAGAAGTCTCAGGCTACGAGTTTGATCCTCAATGGAAAGGCTGTTTGCTGTATGCATATGGGAAACTTTGATGAAGCCGAAACACTACTTCTTGAAGCACTGCAAAAGGATGCAAAGGATCCAGAAACTCTAGCCAACCTTGTTGTATGCTGTCTTCACCTGGGAAAGCCATCTTCTCGTTTCCTCAGCCAGTTGAAAATTGGGCACCCAAATCACTTGCTCGTCAAGCGGGCCGCGGAGGCAGAACAGAACATTAACCAAGCTCTTCAAACAGCTGGTTGA

Protein Analysis

294

Amino Acids

32.21

Weight (kDa)

5.15

Isoelectric Point (pI)

39.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Coatomer_E PF04733 8 - 290 1.2e-128 Coatomer epsilon subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012024)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G30630 AT2G34840 AT2G34840
fragaria_vesca FvH4_3g11400 FvH4_3g11400
malus_domestica MD05G1263700.v1.1 MD10G1241400.v1.1
prunus_persica Prupe.4G101800_v2.0.a1
pyrus_communis pycom05g24770 pycom10g20240
rosa_chinensis RchiOBHm_Chr5g0018341
rosa_laevigata RLG00000032353
rosa_multiflora Rmu_sc0004464.1_g000001
rosa_roxburghii Rroxscaffold_1G00059400
rosa_rugosa Rorug05G0041600
rosa_samantha Rh5BG133500 Rh5CG145300 Rh5DG134900
rosa_wichuraiana Rw5G011780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 119, 137
AccB1I GGYRCC 2 cut(s) 62, 804
AccBSI CCGCTC 1 cut(s) 147
AccII CGCG 3 cut(s) 129, 467, 836
AccIII TCCGGA 1 cut(s) 398
AclWI GGATC 5 cut(s) 296, 309, 619, 713, 726
AcoI YGGCCR 1 cut(s) 306
AcsI RAATTY 1 cut(s) 280
AcyI GRCGYC 3 cut(s) 63, 117, 195
AfiI CCNNNNNNNGG 1 cut(s) 311
AgsI TTSAA 6 cut(s) 262, 286, 427, 695, 796, 869
AjnI CCWGG 3 cut(s) 69, 216, 759
AjuI GAANNNNNNNTTGG 2 cut(s) 278, 310
AluBI AGCT 7 cut(s) 168, 175, 229, 380, 478, 863, 875
AluI AGCT 7 cut(s) 168, 175, 229, 380, 478, 863, 875
Alw26I GTCTC 2 cut(s) 466, 612
AlwI GGATC 5 cut(s) 296, 309, 619, 713, 726
Aor13HI TCCGGA 1 cut(s) 398
AoxI GGCC 5 cut(s) 72, 219, 250, 306, 831
ApeKI GCWGC 3 cut(s) 5, 14, 478
ApoI RAATTY 1 cut(s) 280
AspLEI GCGC 2 cut(s) 65, 129
AspS9I GGNCC 5 cut(s) 20, 25, 251, 505, 831
AsuHPI GGTGA 1 cut(s) 749
AvaII GGWCC 3 cut(s) 20, 25, 505
AxyI CCTNAGG 1 cut(s) 329
BaeGI GKGCMC 1 cut(s) 807
BamHI GGATCC 2 cut(s) 301, 718
BanI GGYRCC 2 cut(s) 62, 804
BarI GAAGNNNNNNTAC 2 cut(s) 738, 770
BbsI GAAGAC 2 cut(s) 133, 746
BbvCI CCTCAGC 1 cut(s) 785
BbvI GCAGC 3 cut(s) 17, 26, 465
BccI CCATC 1 cut(s) 778
BceAI ACGGC 3 cut(s) 104, 206, 293
BciT130I CCWGG 3 cut(s) 71, 218, 761
BcoDI GTCTC 2 cut(s) 466, 612
BfaI CTAG 1 cut(s) 731
BfoI RGCGCY 1 cut(s) 66
BglI GCCNNNNNGGC 2 cut(s) 71, 839
BisI GCNGC 7 cut(s) 6, 15, 75, 204, 479, 482, 834
BlsI GCNGC 7 cut(s) 7, 16, 76, 205, 480, 483, 835
Bme1390I CCNGG 3 cut(s) 71, 218, 761
Bme18I GGWCC 3 cut(s) 20, 25, 505
BmgT120I GGNCC 5 cut(s) 20, 25, 251, 505, 831
BmiI GGNNCC 6 cut(s) 64, 253, 303, 559, 720, 806
BmrFI CCNGG 3 cut(s) 71, 218, 761
BmsI GCATC 1 cut(s) 700
BpiI GAAGAC 2 cut(s) 133, 746
BpmI CTGGAG 1 cut(s) 200
Bpu10I CCTNAGC 1 cut(s) 785
BsaHI GRCGYC 3 cut(s) 63, 117, 195
BsaJI CCNNGG 3 cut(s) 160, 760, 834
BsaWI WCCGGW 2 cut(s) 22, 398
Bsc4I CCNNNNNNNGG 1 cut(s) 311
Bse1I ACTGG 1 cut(s) 790
Bse21I CCTNAGG 1 cut(s) 329
Bse3DI GCAATG 1 cut(s) 534
BseAI TCCGGA 1 cut(s) 398
BseBI CCWGG 3 cut(s) 71, 218, 761
BseDI CCNNGG 3 cut(s) 160, 760, 834
BseGI GGATG 1 cut(s) 715
BseLI CCNNNNNNNGG 1 cut(s) 311
BseMI GCAATG 1 cut(s) 534
BseMII CTCAG 4 cut(s) 320, 591, 623, 799
BseNI ACTGG 1 cut(s) 790
BseRI GAGGAG 2 cut(s) 201, 221
BseSI GKGCMC 1 cut(s) 807
BseX3I CGGCCG 1 cut(s) 306
BseXI GCAGC 3 cut(s) 17, 26, 465
BseYI CCCAGC 1 cut(s) 341
Bsh1236I CGCG 3 cut(s) 129, 467, 836
Bsh1285I CGRYCG 1 cut(s) 309
BshFI GGCC 5 cut(s) 74, 221, 252, 308, 833
BshNI GGYRCC 2 cut(s) 62, 804
BsiEI CGRYCG 1 cut(s) 309
BsiSI CCGG 3 cut(s) 23, 305, 399
BslFI GGGAC 1 cut(s) 358
BslI CCNNNNNNNGG 1 cut(s) 311
BsmAI GTCTC 2 cut(s) 466, 612
BsmFI GGGAC 1 cut(s) 358
BsmI GAATGC 1 cut(s) 424
BsnI GGCC 5 cut(s) 74, 221, 252, 308, 833
Bsp1286I GDGCHC 1 cut(s) 807
Bsp13I TCCGGA 1 cut(s) 398
Bsp143I GATC 5 cut(s) 189, 273, 301, 624, 718
BspANI GGCC 5 cut(s) 74, 221, 252, 308, 833
BspCNI CTCAG 4 cut(s) 321, 592, 622, 798
BspEI TCCGGA 1 cut(s) 398
BspFNI CGCG 3 cut(s) 129, 467, 836
BspLI GGNNCC 6 cut(s) 64, 253, 303, 559, 720, 806
BspPI GGATC 5 cut(s) 296, 309, 619, 713, 726
BspQI GCTCTTC 1 cut(s) 870
BspT107I GGYRCC 2 cut(s) 62, 804
BsrBI CCGCTC 1 cut(s) 147
BsrDI GCAATG 1 cut(s) 534
BsrI ACTGG 1 cut(s) 790
BssECI CCNNGG 3 cut(s) 160, 760, 834
BssMI GATC 5 cut(s) 189, 273, 301, 624, 718
BssNI GRCGYC 3 cut(s) 63, 117, 195
Bst2UI CCWGG 3 cut(s) 71, 218, 761
Bst4CI ACNGT 1 cut(s) 349
Bst6I CTCTTC 1 cut(s) 870
BstACI GRCGYC 3 cut(s) 63, 117, 195
BstC8I GCNNGC 1 cut(s) 831
BstDEI CTNAG 4 cut(s) 329, 600, 609, 785
BstDSI CCRYGG 1 cut(s) 834
BstF5I GGATG 1 cut(s) 715
BstFNI CGCG 3 cut(s) 129, 467, 836
BstH2I RGCGCY 1 cut(s) 66
BstHHI GCGC 2 cut(s) 65, 129
BstKTI GATC 5 cut(s) 192, 276, 304, 627, 721
BstMAI GTCTC 2 cut(s) 466, 612
BstMBI GATC 5 cut(s) 189, 273, 301, 624, 718
BstMCI CGRYCG 1 cut(s) 309
BstMWI GCNNNNNNNGC 8 cut(s) 11, 14, 71, 203, 305, 487, 826, 839
BstNI CCWGG 3 cut(s) 71, 218, 761
BstSCI CCNGG 3 cut(s) 69, 216, 759
BstSLI GKGCMC 1 cut(s) 807
BstUI CGCG 3 cut(s) 129, 467, 836
BstV1I GCAGC 3 cut(s) 17, 26, 465
BstV2I GAAGAC 2 cut(s) 133, 746
BstX2I RGATCY 2 cut(s) 301, 718
BstYI RGATCY 2 cut(s) 301, 718
BstZI CGGCCG 1 cut(s) 306
Bsu36I CCTNAGG 1 cut(s) 329
BsuRI GGCC 5 cut(s) 74, 221, 252, 308, 833
BtgI CCRYGG 1 cut(s) 834
BtgZI GCGATG 1 cut(s) 139
BtsCI GGATG 1 cut(s) 715
BtsI GCAGTG 3 cut(s) 15, 555, 698
BtsIMutI CAGTG 3 cut(s) 15, 555, 698
Cac8I GCNNGC 1 cut(s) 831
CfoI GCGC 2 cut(s) 65, 129
Cfr13I GGNCC 5 cut(s) 20, 25, 251, 505, 831
Cfr42I CCGCGG 1 cut(s) 837
CseI GACGC 2 cut(s) 125, 203
CviAII CATG 4 cut(s) 355, 359, 487, 534
DdeI CTNAG 4 cut(s) 329, 600, 609, 785
DinI GGCGCC 1 cut(s) 64
DpnI GATC 5 cut(s) 191, 275, 303, 626, 720
DpnII GATC 5 cut(s) 189, 273, 301, 624, 718
DrdI GACNNNNNNGTC 2 cut(s) 119, 137
DseDI GACNNNNNNGTC 2 cut(s) 119, 137
EaeI YGGCCR 1 cut(s) 306
EagI CGGCCG 1 cut(s) 306
Eam1104I CTCTTC 1 cut(s) 870
EarI CTCTTC 1 cut(s) 870
EciI GGCGGA 2 cut(s) 190, 314
EclXI CGGCCG 1 cut(s) 306
Eco47I GGWCC 3 cut(s) 20, 25, 505
Eco52I CGGCCG 1 cut(s) 306
Eco81I CCTNAGG 1 cut(s) 329
EcoRII CCWGG 3 cut(s) 69, 216, 759
EcoT22I ATGCAT 4 cut(s) 360, 424, 453, 657
EgeI GGCGCC 1 cut(s) 64
EheI GGCGCC 1 cut(s) 64
FaeI CATG 4 cut(s) 358, 362, 490, 537
FaqI GGGAC 1 cut(s) 358
FatI CATG 4 cut(s) 354, 358, 486, 533
FauI CCCGC 1 cut(s) 822
FauNDI CATATG 1 cut(s) 657
Fnu4HI GCNGC 7 cut(s) 6, 15, 75, 204, 479, 482, 834
FokI GGATG 1 cut(s) 722
Fsp4HI GCNGC 7 cut(s) 6, 15, 75, 204, 479, 482, 834
FspBI CTAG 1 cut(s) 731
GlaI GCGC 2 cut(s) 64, 128
GluI GCNGC 7 cut(s) 6, 15, 75, 204, 479, 482, 834
GsaI CCCAGC 1 cut(s) 345
GsuI CTGGAG 1 cut(s) 200
HaeII RGCGCY 1 cut(s) 66
HaeIII GGCC 5 cut(s) 74, 221, 252, 308, 833
HapII CCGG 3 cut(s) 23, 305, 399
HgaI GACGC 2 cut(s) 125, 203
HhaI GCGC 2 cut(s) 65, 129
Hin1I GRCGYC 3 cut(s) 63, 117, 195
Hin1II CATG 4 cut(s) 358, 362, 490, 537
Hin6I GCGC 2 cut(s) 63, 127
HinP1I GCGC 2 cut(s) 63, 127
HincII GTYRAC 1 cut(s) 499
HindII GTYRAC 1 cut(s) 499
HinfI GANTC 1 cut(s) 266
HpaII CCGG 3 cut(s) 23, 305, 399
HphI GGTGA 1 cut(s) 749
Hpy166II GTNNAC 1 cut(s) 499
Hpy188I TCNGA 4 cut(s) 164, 314, 460, 601
Hpy188III TCNNGA 5 cut(s) 277, 399, 433, 692, 722
Hpy8I GTNNAC 1 cut(s) 499
Hpy99I CGWCG 5 cut(s) 116, 119, 125, 197, 314
HpyAV CCTTC 1 cut(s) 280
HpyCH4III ACNGT 1 cut(s) 349
HpyCH4V TGCA 8 cut(s) 358, 422, 451, 490, 527, 655, 703, 713
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 14, 71, 203, 305, 487, 826, 839
HpyF3I CTNAG 4 cut(s) 329, 600, 609, 785
Hsp92I GRCGYC 3 cut(s) 63, 117, 195
Hsp92II CATG 4 cut(s) 358, 362, 490, 537
HspAI GCGC 2 cut(s) 63, 127
KasI GGCGCC 1 cut(s) 62
Kpn2I TCCGGA 1 cut(s) 398
KspI CCGCGG 1 cut(s) 837
Kzo9I GATC 5 cut(s) 189, 273, 301, 624, 718
LguI GCTCTTC 1 cut(s) 870
LmnI GCTCC 1 cut(s) 234
Lsp1109I GCAGC 3 cut(s) 17, 26, 465
LweI GCATC 1 cut(s) 700
MaeI CTAG 1 cut(s) 731
MalI GATC 5 cut(s) 191, 275, 303, 626, 720
MbiI CCGCTC 1 cut(s) 147
MboI GATC 5 cut(s) 189, 273, 301, 624, 718
MboII GAAGA 5 cut(s) 133, 577, 746, 765, 857
MflI RGATCY 2 cut(s) 301, 718
MhlI GDGCHC 1 cut(s) 807
MluCI AATT 3 cut(s) 280, 394, 798
Mly113I GGCGCC 1 cut(s) 63
MmeI TCCRAC 1 cut(s) 292
Mph1103I ATGCAT 4 cut(s) 360, 424, 453, 657
MroI TCCGGA 1 cut(s) 398
MseI TTAA 5 cut(s) 36, 81, 444, 539, 855
MspA1I CMGCKG 3 cut(s) 478, 836, 875
MspI CCGG 3 cut(s) 23, 305, 399
MspR9I CCNGG 3 cut(s) 71, 218, 761
Mva1269I GAATGC 1 cut(s) 424
MvaI CCWGG 3 cut(s) 71, 218, 761
MvnI CGCG 3 cut(s) 129, 467, 836
MwoI GCNNNNNNNGC 8 cut(s) 11, 14, 71, 203, 305, 487, 826, 839
NarI GGCGCC 1 cut(s) 63
NdeI CATATG 1 cut(s) 657
NdeII GATC 5 cut(s) 189, 273, 301, 624, 718
NlaIII CATG 4 cut(s) 358, 362, 490, 537
NlaIV GGNNCC 6 cut(s) 64, 253, 303, 559, 720, 806
NsiI ATGCAT 4 cut(s) 360, 424, 453, 657
PciSI GCTCTTC 1 cut(s) 870
PcsI WCGNNNNNNNCGW 3 cut(s) 120, 183, 275
PctI GAATGC 1 cut(s) 424
PfeI GAWTC 1 cut(s) 266
PflFI GACNNNGTC 1 cut(s) 119
PkrI GCNGC 7 cut(s) 7, 16, 76, 205, 480, 483, 835
PluTI GGCGCC 1 cut(s) 66
Psp6I CCWGG 3 cut(s) 69, 216, 759
PspFI CCCAGC 1 cut(s) 341
PspGI CCWGG 3 cut(s) 69, 216, 759
PspN4I GGNNCC 6 cut(s) 64, 253, 303, 559, 720, 806
PspPI GGNCC 5 cut(s) 20, 25, 251, 505, 831
PsuI RGATCY 2 cut(s) 301, 718
PsyI GACNNNGTC 1 cut(s) 119
PvuII CAGCTG 2 cut(s) 478, 875
SacII CCGCGG 1 cut(s) 837
SapI GCTCTTC 1 cut(s) 870
SaqAI TTAA 5 cut(s) 36, 81, 444, 539, 855
SatI GCNGC 7 cut(s) 6, 15, 75, 204, 479, 482, 834
Sau3AI GATC 5 cut(s) 189, 273, 301, 624, 718
Sau96I GGNCC 5 cut(s) 20, 25, 251, 505, 831
ScrFI CCNGG 3 cut(s) 71, 218, 761
SduI GDGCHC 1 cut(s) 807
SfaNI GCATC 1 cut(s) 700
SfoI GGCGCC 1 cut(s) 64
Sfr303I CCGCGG 1 cut(s) 837
SgrBI CCGCGG 1 cut(s) 837
SinI GGWCC 3 cut(s) 20, 25, 505
Sse9I AATT 3 cut(s) 280, 394, 798
SspDI GGCGCC 1 cut(s) 62
SspMI CTAG 1 cut(s) 731
StyD4I CCNGG 3 cut(s) 69, 216, 759
TaaI ACNGT 1 cut(s) 349
TaqI TCGA 3 cut(s) 123, 192, 278
TasI AATT 3 cut(s) 280, 394, 798
TauI GCSGC 4 cut(s) 77, 206, 484, 836
TfiI GAWTC 1 cut(s) 266
Tru1I TTAA 5 cut(s) 36, 81, 444, 539, 855
Tru9I TTAA 5 cut(s) 36, 81, 444, 539, 855
TscAI CASTG 3 cut(s) 15, 555, 705
TseI GCWGC 3 cut(s) 5, 14, 478
TspDTI ATGAA 3 cut(s) 343, 390, 687
TspRI CASTG 3 cut(s) 15, 555, 705
Tth111I GACNNNGTC 1 cut(s) 119
VpaK11BI GGWCC 3 cut(s) 20, 25, 505
XapI RAATTY 1 cut(s) 280
XcmI CCANNNNNNNNNTGG 1 cut(s) 797
XspI CTAG 1 cut(s) 731
Zsp2I ATGCAT 4 cut(s) 360, 424, 453, 657
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.