RchiOBHm_Chr5g0018341

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. The coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0012024
Physical Location & Seq
Reverse (-)
12916372 .. 12918964
2593 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29856

Sequence Viewer

Length: 885 bp
ATGGCAGCACTAGCAGCAGGACCGGACCACCTCTTCAACCTCCGCAACAGCTTCTACTTGGGGGCCTACCAGGCCGCCATTAACACCAGCGACATCCCCAACCTCTCGCCGGACGAAGCCGTCGAGCGCGACTGCCTCGTTTTCCGATCCTACATAGCCCTCGGTAGCTACCAGCTCGTCATCAACGAGATCGACTCCTCCGCCGCCACGCCTCTCCAGGCCGTCAAGCTCCTCGCCCTCTACCTCTCCGGCCCCGATTACAAGGAATCGGCGATCTCGAATCTAAAGGAGTTGCTGGCGGATCCGGCCATCGGGAACAACGCGACTCTGAGGATGATCGCCGGGACTGTATTTATGCATGAGCAGGACTACAATGAAGCTCTCAAGTACACCCATGCTTCTGGAGGCAATTTGGAACTGGATGCACTGAATGTCCAAATATTCCTCAAAATGCATAGGTCTGATTATGCTGAAAGACGGCTGAAGGAAATGCAAAAGATTGATGAGGACCACACCCTAACTCAACTTGCAACAGCATGGCAAAATCTGGCAGTGGGTGGTTCTAAGATACAGGAGGCATATCTTATCTTCCAAGATTTCTCTGAAAAGTCTCAGGTCACAAGCTTGATTTTGAACGGAAAGGCTGTTTGCTGCATACACATGGGAAACTTTGATGAAGCTGAAACACTGCTTCTTGAAGCACTGAACAAGGATGCAAAGGATCCAGAAACTCTAGCCAACCTTGTCGTATGCTGTCTTCACCTTGGAAAACCACCTTCTCGTTTTCTCAGCCAATTAAGACTTGCACATCCAGACCACGTCCTCGTCAAACGTGCCTCAGCTGCAGAAGAGAGCTTTGACAGAGCTGTTCAGTCAGTTGCTTGA

Protein Analysis

294

Amino Acids

32.37

Weight (kDa)

5.18

Isoelectric Point (pI)

37.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Coatomer_E PF04733 8 - 293 3.1e-131 Coatomer epsilon subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012024)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G30630 AT2G34840 AT2G34840
fragaria_vesca FvH4_3g11400 FvH4_3g11400
malus_domestica MD05G1263700.v1.1 MD10G1241400.v1.1
prunus_persica Prupe.4G101800_v2.0.a1
pyrus_communis pycom05g24770 pycom10g20240
rosa_chinensis RchiOBHm_Chr5g0018341
rosa_laevigata RLG00000032353
rosa_multiflora Rmu_sc0004464.1_g000001
rosa_roxburghii Rroxscaffold_1G00059400
rosa_rugosa Rorug05G0041600
rosa_samantha Rh5BG133500 Rh5CG145300 Rh5DG134900
rosa_wichuraiana Rw5G011780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 119
AccII CGCG 2 cut(s) 129, 323
AciI CCGC 5 cut(s) 43, 75, 201, 204, 299
AclWI GGATC 5 cut(s) 141, 296, 309, 716, 729
AcoI YGGCCR 1 cut(s) 306
AcuI CTGAAG 1 cut(s) 503
AfaI GTAC 1 cut(s) 389
AfiI CCNNNNNNNGG 2 cut(s) 109, 311
AgsI TTSAA 3 cut(s) 37, 634, 698
AjiI CACGTC 1 cut(s) 820
AjnI CCWGG 2 cut(s) 69, 216
Alw26I GTCTC 1 cut(s) 615
AlwI GGATC 5 cut(s) 141, 296, 309, 716, 729
AoxI GGCC 5 cut(s) 63, 72, 219, 250, 306
ApeKI GCWGC 4 cut(s) 5, 14, 651, 842
AspLEI GCGC 1 cut(s) 129
AspS9I GGNCC 5 cut(s) 20, 25, 63, 251, 508
AsuC2I CCSGG 1 cut(s) 343
AsuHPI GGTGA 1 cut(s) 752
AvaII GGWCC 3 cut(s) 20, 25, 508
BamHI GGATCC 2 cut(s) 301, 721
BarI GAAGNNNNNNTAC 2 cut(s) 741, 773
BbsI GAAGAC 1 cut(s) 749
BbvCI CCTCAGC 1 cut(s) 838
BbvI GCAGC 4 cut(s) 17, 26, 638, 829
BccI CCATC 1 cut(s) 317
BceAI ACGGC 3 cut(s) 104, 206, 494
BciT130I CCWGG 2 cut(s) 71, 218
BcnI CCSGG 1 cut(s) 343
BcoDI GTCTC 1 cut(s) 615
BfaI CTAG 2 cut(s) 11, 734
BfmI CTRYAG 1 cut(s) 843
BglI GCCNNNNNGGC 1 cut(s) 71
BisI GCNGC 6 cut(s) 6, 15, 75, 204, 652, 843
BlsI GCNGC 6 cut(s) 7, 16, 76, 205, 653, 844
Bme1390I CCNGG 3 cut(s) 71, 218, 343
Bme18I GGWCC 3 cut(s) 20, 25, 508
BmgBI CACGTC 1 cut(s) 820
BmgT120I GGNCC 5 cut(s) 20, 25, 63, 251, 508
BmiI GGNNCC 4 cut(s) 64, 253, 303, 723
BmrFI CCNGG 3 cut(s) 71, 218, 343
BmsI GCATC 2 cut(s) 412, 703
BpiI GAAGAC 1 cut(s) 749
BplI GAGNNNNNCTC 2 cut(s) 179, 211
BpmI CTGGAG 2 cut(s) 200, 423
Bpu10I CCTNAGC 1 cut(s) 838
BpuEI CTTGAG 1 cut(s) 368
BpuMI CCSGG 1 cut(s) 343
BsaJI CCNNGG 2 cut(s) 160, 763
BsaWI WCCGGW 1 cut(s) 22
Bsc4I CCNNNNNNNGG 2 cut(s) 109, 311
Bse1I ACTGG 1 cut(s) 423
BseBI CCWGG 2 cut(s) 71, 218
BseDI CCNNGG 2 cut(s) 160, 763
BseGI GGATG 5 cut(s) 93, 339, 427, 718, 808
BseLI CCNNNNNNNGG 2 cut(s) 109, 311
BseMII CTCAG 4 cut(s) 320, 626, 802, 852
BseNI ACTGG 1 cut(s) 423
BseRI GAGGAG 2 cut(s) 187, 221
BseXI GCAGC 4 cut(s) 17, 26, 638, 829
Bsh1236I CGCG 2 cut(s) 129, 323
BshFI GGCC 5 cut(s) 65, 74, 221, 252, 308
BsiSI CCGG 5 cut(s) 23, 110, 249, 305, 342
BslFI GGGAC 1 cut(s) 358
BslI CCNNNNNNNGG 2 cut(s) 109, 311
BsmAI GTCTC 1 cut(s) 615
BsmFI GGGAC 1 cut(s) 358
BsnI GGCC 5 cut(s) 65, 74, 221, 252, 308
Bsp143I GATC 6 cut(s) 146, 189, 273, 301, 336, 721
BspACI CCGC 5 cut(s) 43, 75, 201, 204, 299
BspANI GGCC 5 cut(s) 65, 74, 221, 252, 308
BspCNI CTCAG 4 cut(s) 321, 625, 801, 851
BspFNI CGCG 2 cut(s) 129, 323
BspLI GGNNCC 4 cut(s) 64, 253, 303, 723
BspMAI CTGCAG 1 cut(s) 847
BspPI GGATC 5 cut(s) 141, 296, 309, 716, 729
BsrI ACTGG 1 cut(s) 423
BssECI CCNNGG 2 cut(s) 160, 763
BssMI GATC 6 cut(s) 146, 189, 273, 301, 336, 721
BssT1I CCWWGG 1 cut(s) 763
Bst2UI CCWGG 2 cut(s) 71, 218
Bst4CI ACNGT 1 cut(s) 349
Bst6I CTCTTC 2 cut(s) 38, 843
BstC8I GCNNGC 1 cut(s) 297
BstDEI CTNAG 5 cut(s) 329, 564, 612, 788, 838
BstF5I GGATG 5 cut(s) 93, 339, 427, 718, 808
BstFNI CGCG 2 cut(s) 129, 323
BstHHI GCGC 1 cut(s) 129
BstKTI GATC 6 cut(s) 149, 192, 276, 304, 339, 724
BstMAI GTCTC 1 cut(s) 615
BstMBI GATC 6 cut(s) 146, 189, 273, 301, 336, 721
BstMWI GCNNNNNNNGC 5 cut(s) 11, 14, 71, 305, 842
BstNI CCWGG 2 cut(s) 71, 218
BstSCI CCNGG 3 cut(s) 69, 216, 341
BstSFI CTRYAG 1 cut(s) 843
BstUI CGCG 2 cut(s) 129, 323
BstV1I GCAGC 4 cut(s) 17, 26, 638, 829
BstV2I GAAGAC 1 cut(s) 749
BstX2I RGATCY 2 cut(s) 301, 721
BstXI CCANNNNNNTGG 1 cut(s) 401
BstYI RGATCY 2 cut(s) 301, 721
BsuRI GGCC 5 cut(s) 65, 74, 221, 252, 308
BtrI CACGTC 1 cut(s) 820
BtsCI GGATG 5 cut(s) 93, 339, 427, 718, 808
BtsI GCAGTG 2 cut(s) 558, 686
BtsIMutI CAGTG 4 cut(s) 425, 558, 686, 701
Cac8I GCNNGC 1 cut(s) 297
CfoI GCGC 1 cut(s) 129
Cfr13I GGNCC 5 cut(s) 20, 25, 63, 251, 508
Csp6I GTAC 1 cut(s) 388
CviAII CATG 4 cut(s) 359, 395, 537, 661
CviQI GTAC 1 cut(s) 388
DdeI CTNAG 5 cut(s) 329, 564, 612, 788, 838
DpnI GATC 6 cut(s) 148, 191, 275, 303, 338, 723
DpnII GATC 6 cut(s) 146, 189, 273, 301, 336, 721
DrdI GACNNNNNNGTC 1 cut(s) 119
DseDI GACNNNNNNGTC 1 cut(s) 119
EaeI YGGCCR 1 cut(s) 306
Eam1104I CTCTTC 2 cut(s) 38, 843
EarI CTCTTC 2 cut(s) 38, 843
EciI GGCGGA 2 cut(s) 190, 314
Eco130I CCWWGG 1 cut(s) 763
Eco47I GGWCC 3 cut(s) 20, 25, 508
Eco57I CTGAAG 1 cut(s) 503
EcoO109I RGGNCCY 1 cut(s) 63
EcoRII CCWGG 2 cut(s) 69, 216
EcoT14I CCWWGG 1 cut(s) 763
EcoT22I ATGCAT 2 cut(s) 360, 456
ErhI CCWWGG 1 cut(s) 763
FaeI CATG 4 cut(s) 362, 398, 540, 664
FaqI GGGAC 1 cut(s) 358
FatI CATG 4 cut(s) 358, 394, 536, 660
Fnu4HI GCNGC 6 cut(s) 6, 15, 75, 204, 652, 843
FokI GGATG 5 cut(s) 80, 346, 434, 725, 795
Fsp4HI GCNGC 6 cut(s) 6, 15, 75, 204, 652, 843
FspBI CTAG 2 cut(s) 11, 734
GlaI GCGC 1 cut(s) 128
GluI GCNGC 6 cut(s) 6, 15, 75, 204, 652, 843
GsuI CTGGAG 2 cut(s) 200, 423
HaeIII GGCC 5 cut(s) 65, 74, 221, 252, 308
HapII CCGG 5 cut(s) 23, 110, 249, 305, 342
HhaI GCGC 1 cut(s) 129
Hin1II CATG 4 cut(s) 362, 398, 540, 664
Hin6I GCGC 1 cut(s) 127
HinP1I GCGC 1 cut(s) 127
HindIII AAGCTT 1 cut(s) 622
HinfI GANTC 4 cut(s) 194, 266, 280, 325
HpaII CCGG 5 cut(s) 23, 110, 249, 305, 342
HphI GGTGA 1 cut(s) 752
Hpy166II GTNNAC 1 cut(s) 390
Hpy188I TCNGA 4 cut(s) 146, 330, 463, 604
Hpy188III TCNNGA 6 cut(s) 277, 313, 402, 695, 725, 812
Hpy8I GTNNAC 1 cut(s) 390
Hpy99I CGWCG 1 cut(s) 125
HpyAV CCTTC 2 cut(s) 478, 786
HpyCH4III ACNGT 1 cut(s) 349
HpyCH4IV ACGT 2 cut(s) 819, 832
HpyCH4V TGCA 9 cut(s) 358, 425, 454, 493, 530, 654, 716, 806, 845
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 14, 71, 305, 842
HpyF3I CTNAG 5 cut(s) 329, 564, 612, 788, 838
HpySE526I ACGT 2 cut(s) 819, 832
Hsp92II CATG 4 cut(s) 362, 398, 540, 664
HspAI GCGC 1 cut(s) 127
Kzo9I GATC 6 cut(s) 146, 189, 273, 301, 336, 721
LmnI GCTCC 1 cut(s) 234
Lsp1109I GCAGC 4 cut(s) 17, 26, 638, 829
LweI GCATC 2 cut(s) 412, 703
MaeI CTAG 2 cut(s) 11, 734
MaeII ACGT 2 cut(s) 819, 832
MaeIII GTNAC 1 cut(s) 616
MalI GATC 6 cut(s) 148, 191, 275, 303, 338, 723
MboI GATC 6 cut(s) 146, 189, 273, 301, 336, 721
MboII GAAGA 4 cut(s) 25, 580, 749, 860
MflI RGATCY 2 cut(s) 301, 721
MluCI AATT 2 cut(s) 409, 794
MlyI GAGTC 2 cut(s) 188, 319
Mph1103I ATGCAT 2 cut(s) 360, 456
MseI TTAA 2 cut(s) 81, 797
MslI CAYNNNNRTG 1 cut(s) 659
MspA1I CMGCKG 1 cut(s) 842
MspI CCGG 5 cut(s) 23, 110, 249, 305, 342
MspR9I CCNGG 3 cut(s) 71, 218, 343
MvaI CCWGG 2 cut(s) 71, 218
MvnI CGCG 2 cut(s) 129, 323
MwoI GCNNNNNNNGC 5 cut(s) 11, 14, 71, 305, 842
NciI CCSGG 1 cut(s) 343
NdeII GATC 6 cut(s) 146, 189, 273, 301, 336, 721
NlaIII CATG 4 cut(s) 362, 398, 540, 664
NlaIV GGNNCC 4 cut(s) 64, 253, 303, 723
NmuCI GTSAC 1 cut(s) 616
NsiI ATGCAT 2 cut(s) 360, 456
PcsI WCGNNNNNNNCGW 3 cut(s) 120, 183, 275
PfeI GAWTC 2 cut(s) 266, 280
PflFI GACNNNGTC 1 cut(s) 818
PkrI GCNGC 6 cut(s) 7, 16, 76, 205, 653, 844
PleI GAGTC 2 cut(s) 188, 319
PpsI GAGTC 2 cut(s) 188, 319
Psp6I CCWGG 2 cut(s) 69, 216
PspGI CCWGG 2 cut(s) 69, 216
PspN4I GGNNCC 4 cut(s) 64, 253, 303, 723
PspPI GGNCC 5 cut(s) 20, 25, 63, 251, 508
PstI CTGCAG 1 cut(s) 847
PsuI RGATCY 2 cut(s) 301, 721
PsyI GACNNNGTC 1 cut(s) 818
PvuII CAGCTG 1 cut(s) 842
RsaI GTAC 1 cut(s) 389
RsaNI GTAC 1 cut(s) 388
RseI CAYNNNNRTG 1 cut(s) 659
SaqAI TTAA 2 cut(s) 81, 797
SatI GCNGC 6 cut(s) 6, 15, 75, 204, 652, 843
Sau3AI GATC 6 cut(s) 146, 189, 273, 301, 336, 721
Sau96I GGNCC 5 cut(s) 20, 25, 63, 251, 508
SchI GAGTC 2 cut(s) 188, 319
ScrFI CCNGG 3 cut(s) 71, 218, 343
SfaNI GCATC 2 cut(s) 412, 703
SfcI CTRYAG 1 cut(s) 843
SfiI GGCCNNNNNGGCC 1 cut(s) 71
SinI GGWCC 3 cut(s) 20, 25, 508
SmiMI CAYNNNNRTG 1 cut(s) 659
SmlI CTYRAG 1 cut(s) 383
SmoI CTYRAG 1 cut(s) 383
Sse9I AATT 2 cut(s) 409, 794
SsiI CCGC 5 cut(s) 43, 75, 201, 204, 299
SspI AATATT 1 cut(s) 441
SspMI CTAG 2 cut(s) 11, 734
StyD4I CCNGG 3 cut(s) 69, 216, 341
StyI CCWWGG 1 cut(s) 763
TaaI ACNGT 1 cut(s) 349
TaiI ACGT 2 cut(s) 822, 835
TaqI TCGA 3 cut(s) 123, 192, 278
TasI AATT 2 cut(s) 409, 794
TatI WGTACW 1 cut(s) 387
TauI GCSGC 2 cut(s) 77, 206
TfiI GAWTC 2 cut(s) 266, 280
Tru1I TTAA 2 cut(s) 81, 797
Tru9I TTAA 2 cut(s) 81, 797
TscAI CASTG 4 cut(s) 432, 558, 693, 708
TseFI GTSAC 1 cut(s) 616
TseI GCWGC 4 cut(s) 5, 14, 651, 842
Tsp45I GTSAC 1 cut(s) 616
TspDTI ATGAA 2 cut(s) 390, 690
TspGWI ACGGA 1 cut(s) 651
TspRI CASTG 4 cut(s) 432, 558, 693, 708
Tth111I GACNNNGTC 1 cut(s) 818
VpaK11BI GGWCC 3 cut(s) 20, 25, 508
XspI CTAG 2 cut(s) 11, 734
Zsp2I ATGCAT 2 cut(s) 360, 456
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.