Rh5CG145300

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. The coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0012024
Physical Location & Seq
Reverse (-)
13082108 .. 13084685
2578 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG145300.1

Sequence Viewer

Length: 729 bp
ATGGCAGCACTAGCAGCAGGACCGGACCACCTCTTCAACCTCCGCAACAGCTTCTACTTGGGGGCCTACCAGGCCGCCATTAACACCAGCGACATCCCCAACCTCTCGCCGGACGAAGCCGTCGAGCGCGACTGCCTCGTTTTCCGATCCTACATAGCCCTCGGTAGCTACCAGCTCGTCATCAACGAGATCGACTCCTCCGCCGCCACGCCTCTCCAGGCCGTCAAGCTCCTCGCCCTCTACCTCTCCGGCCCCGATTACAAGGAATCGGCGATCTCGAATCTAAAGGAGTTGCTGGCGGATCCGGCCATCGGGAACAACGCGACTCTGAGGATGATCGCCGGGACTGTATTTATGCATGAGCAGGACTACAATGAAGCTCTCAAGTACACCCATGCTTCTGGAGGCAATTTGGAACTGGATGCACTGAATGTCCAAATATTCCTCAAAATGCATAGGTCTGATTATGCTGAAAGACGGCTGAAGGAAATGCAAAAGATTGATGAGGACCACACCCTAACTCAACTTGCAACAGCATGGCAAAATCTGGCAGTGGGTGGTTCTAAGATACAGGAGGCATATCTTATCTTCCAAGATTTCTCTGAAAAGTCTCAGGTCACAAGCTTGATTTTGAACGGAAAGGCTGTTTGCTGCATACACATGGGAAACTTTGATGAAGCTGAAACACTGCTTCTTGAAGCACTGAACAAGGCAAGTCTGATTAATTAG

Protein Analysis

242

Amino Acids

26.68

Weight (kDa)

4.86

Isoelectric Point (pI)

34.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Coatomer_E PF04733 8 - 238 3.4e-106 Coatomer epsilon subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012024)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G30630 AT2G34840 AT2G34840
fragaria_vesca FvH4_3g11400 FvH4_3g11400
malus_domestica MD05G1263700.v1.1 MD10G1241400.v1.1
prunus_persica Prupe.4G101800_v2.0.a1
pyrus_communis pycom05g24770 pycom10g20240
rosa_chinensis RchiOBHm_Chr5g0018341
rosa_laevigata RLG00000032353
rosa_multiflora Rmu_sc0004464.1_g000001
rosa_roxburghii Rroxscaffold_1G00059400
rosa_rugosa Rorug05G0041600
rosa_samantha Rh5BG133500 Rh5CG145300 Rh5DG134900
rosa_wichuraiana Rw5G011780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 119
AccII CGCG 2 cut(s) 129, 323
AciI CCGC 5 cut(s) 43, 75, 201, 204, 299
AclWI GGATC 3 cut(s) 141, 296, 309
AcoI YGGCCR 1 cut(s) 306
AcuI CTGAAG 1 cut(s) 503
AfaI GTAC 1 cut(s) 389
AfiI CCNNNNNNNGG 2 cut(s) 109, 311
AgsI TTSAA 3 cut(s) 37, 634, 698
AjnI CCWGG 2 cut(s) 69, 216
AluBI AGCT 7 cut(s) 51, 168, 175, 229, 380, 624, 680
AluI AGCT 7 cut(s) 51, 168, 175, 229, 380, 624, 680
Alw26I GTCTC 1 cut(s) 615
AlwI GGATC 3 cut(s) 141, 296, 309
AoxI GGCC 5 cut(s) 63, 72, 219, 250, 306
ApeKI GCWGC 3 cut(s) 5, 14, 651
AseI ATTAAT 1 cut(s) 723
AspLEI GCGC 1 cut(s) 129
AspS9I GGNCC 5 cut(s) 20, 25, 63, 251, 508
AsuC2I CCSGG 1 cut(s) 343
AvaII GGWCC 3 cut(s) 20, 25, 508
BamHI GGATCC 1 cut(s) 301
BbvI GCAGC 3 cut(s) 17, 26, 638
BccI CCATC 1 cut(s) 317
BceAI ACGGC 3 cut(s) 104, 206, 494
BciT130I CCWGG 2 cut(s) 71, 218
BcnI CCSGG 1 cut(s) 343
BcoDI GTCTC 1 cut(s) 615
BfaI CTAG 1 cut(s) 11
BglI GCCNNNNNGGC 1 cut(s) 71
BisI GCNGC 5 cut(s) 6, 15, 75, 204, 652
BlsI GCNGC 5 cut(s) 7, 16, 76, 205, 653
Bme1390I CCNGG 3 cut(s) 71, 218, 343
Bme18I GGWCC 3 cut(s) 20, 25, 508
BmgT120I GGNCC 5 cut(s) 20, 25, 63, 251, 508
BmiI GGNNCC 3 cut(s) 64, 253, 303
BmrFI CCNGG 3 cut(s) 71, 218, 343
BmsI GCATC 1 cut(s) 412
BplI GAGNNNNNCTC 2 cut(s) 179, 211
BpmI CTGGAG 2 cut(s) 200, 423
BpuEI CTTGAG 1 cut(s) 368
BpuMI CCSGG 1 cut(s) 343
BsaJI CCNNGG 1 cut(s) 160
BsaWI WCCGGW 1 cut(s) 22
Bsc4I CCNNNNNNNGG 2 cut(s) 109, 311
Bse1I ACTGG 1 cut(s) 423
BseBI CCWGG 2 cut(s) 71, 218
BseDI CCNNGG 1 cut(s) 160
BseGI GGATG 3 cut(s) 93, 339, 427
BseLI CCNNNNNNNGG 2 cut(s) 109, 311
BseMII CTCAG 2 cut(s) 320, 626
BseNI ACTGG 1 cut(s) 423
BseRI GAGGAG 2 cut(s) 187, 221
BseXI GCAGC 3 cut(s) 17, 26, 638
Bsh1236I CGCG 2 cut(s) 129, 323
BshFI GGCC 5 cut(s) 65, 74, 221, 252, 308
BsiSI CCGG 5 cut(s) 23, 110, 249, 305, 342
BslFI GGGAC 1 cut(s) 358
BslI CCNNNNNNNGG 2 cut(s) 109, 311
BsmAI GTCTC 1 cut(s) 615
BsmFI GGGAC 1 cut(s) 358
BsnI GGCC 5 cut(s) 65, 74, 221, 252, 308
Bsp143I GATC 5 cut(s) 146, 189, 273, 301, 336
BspACI CCGC 5 cut(s) 43, 75, 201, 204, 299
BspANI GGCC 5 cut(s) 65, 74, 221, 252, 308
BspCNI CTCAG 2 cut(s) 321, 625
BspFNI CGCG 2 cut(s) 129, 323
BspLI GGNNCC 3 cut(s) 64, 253, 303
BspPI GGATC 3 cut(s) 141, 296, 309
BsrI ACTGG 1 cut(s) 423
BssECI CCNNGG 1 cut(s) 160
BssMI GATC 5 cut(s) 146, 189, 273, 301, 336
Bst2UI CCWGG 2 cut(s) 71, 218
Bst4CI ACNGT 1 cut(s) 349
Bst6I CTCTTC 1 cut(s) 38
BstC8I GCNNGC 1 cut(s) 297
BstDEI CTNAG 3 cut(s) 329, 564, 612
BstF5I GGATG 3 cut(s) 93, 339, 427
BstFNI CGCG 2 cut(s) 129, 323
BstHHI GCGC 1 cut(s) 129
BstKTI GATC 5 cut(s) 149, 192, 276, 304, 339
BstMAI GTCTC 1 cut(s) 615
BstMBI GATC 5 cut(s) 146, 189, 273, 301, 336
BstMWI GCNNNNNNNGC 4 cut(s) 11, 14, 71, 305
BstNI CCWGG 2 cut(s) 71, 218
BstSCI CCNGG 3 cut(s) 69, 216, 341
BstUI CGCG 2 cut(s) 129, 323
BstV1I GCAGC 3 cut(s) 17, 26, 638
BstX2I RGATCY 1 cut(s) 301
BstXI CCANNNNNNTGG 1 cut(s) 401
BstYI RGATCY 1 cut(s) 301
BsuRI GGCC 5 cut(s) 65, 74, 221, 252, 308
BtsCI GGATG 3 cut(s) 93, 339, 427
BtsI GCAGTG 2 cut(s) 558, 686
BtsIMutI CAGTG 4 cut(s) 425, 558, 686, 701
Cac8I GCNNGC 1 cut(s) 297
CfoI GCGC 1 cut(s) 129
Cfr13I GGNCC 5 cut(s) 20, 25, 63, 251, 508
Csp6I GTAC 1 cut(s) 388
CviAII CATG 4 cut(s) 359, 395, 537, 661
CviQI GTAC 1 cut(s) 388
DdeI CTNAG 3 cut(s) 329, 564, 612
DpnI GATC 5 cut(s) 148, 191, 275, 303, 338
DpnII GATC 5 cut(s) 146, 189, 273, 301, 336
DrdI GACNNNNNNGTC 1 cut(s) 119
DseDI GACNNNNNNGTC 1 cut(s) 119
EaeI YGGCCR 1 cut(s) 306
Eam1104I CTCTTC 1 cut(s) 38
EarI CTCTTC 1 cut(s) 38
EciI GGCGGA 2 cut(s) 190, 314
Eco47I GGWCC 3 cut(s) 20, 25, 508
Eco57I CTGAAG 1 cut(s) 503
EcoO109I RGGNCCY 1 cut(s) 63
EcoRII CCWGG 2 cut(s) 69, 216
EcoT22I ATGCAT 2 cut(s) 360, 456
FaeI CATG 4 cut(s) 362, 398, 540, 664
FaqI GGGAC 1 cut(s) 358
FatI CATG 4 cut(s) 358, 394, 536, 660
Fnu4HI GCNGC 5 cut(s) 6, 15, 75, 204, 652
FokI GGATG 3 cut(s) 80, 346, 434
Fsp4HI GCNGC 5 cut(s) 6, 15, 75, 204, 652
FspBI CTAG 1 cut(s) 11
GlaI GCGC 1 cut(s) 128
GluI GCNGC 5 cut(s) 6, 15, 75, 204, 652
GsuI CTGGAG 2 cut(s) 200, 423
HaeIII GGCC 5 cut(s) 65, 74, 221, 252, 308
HapII CCGG 5 cut(s) 23, 110, 249, 305, 342
HhaI GCGC 1 cut(s) 129
Hin1II CATG 4 cut(s) 362, 398, 540, 664
Hin6I GCGC 1 cut(s) 127
HinP1I GCGC 1 cut(s) 127
HindIII AAGCTT 1 cut(s) 622
HinfI GANTC 4 cut(s) 194, 266, 280, 325
HpaII CCGG 5 cut(s) 23, 110, 249, 305, 342
Hpy166II GTNNAC 1 cut(s) 390
Hpy188I TCNGA 5 cut(s) 146, 330, 463, 604, 720
Hpy188III TCNNGA 4 cut(s) 277, 313, 402, 695
Hpy8I GTNNAC 1 cut(s) 390
Hpy99I CGWCG 1 cut(s) 125
HpyAV CCTTC 1 cut(s) 478
HpyCH4III ACNGT 1 cut(s) 349
HpyCH4V TGCA 6 cut(s) 358, 425, 454, 493, 530, 654
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 14, 71, 305
HpyF3I CTNAG 3 cut(s) 329, 564, 612
Hsp92II CATG 4 cut(s) 362, 398, 540, 664
HspAI GCGC 1 cut(s) 127
Kzo9I GATC 5 cut(s) 146, 189, 273, 301, 336
LmnI GCTCC 1 cut(s) 234
Lsp1109I GCAGC 3 cut(s) 17, 26, 638
LweI GCATC 1 cut(s) 412
MaeI CTAG 1 cut(s) 11
MaeIII GTNAC 1 cut(s) 616
MalI GATC 5 cut(s) 148, 191, 275, 303, 338
MboI GATC 5 cut(s) 146, 189, 273, 301, 336
MboII GAAGA 2 cut(s) 25, 580
MflI RGATCY 1 cut(s) 301
MluCI AATT 2 cut(s) 409, 724
MlyI GAGTC 2 cut(s) 188, 319
Mph1103I ATGCAT 2 cut(s) 360, 456
MseI TTAA 2 cut(s) 81, 723
MslI CAYNNNNRTG 1 cut(s) 659
MspI CCGG 5 cut(s) 23, 110, 249, 305, 342
MspR9I CCNGG 3 cut(s) 71, 218, 343
MvaI CCWGG 2 cut(s) 71, 218
MvnI CGCG 2 cut(s) 129, 323
MwoI GCNNNNNNNGC 4 cut(s) 11, 14, 71, 305
NciI CCSGG 1 cut(s) 343
NdeII GATC 5 cut(s) 146, 189, 273, 301, 336
NlaIII CATG 4 cut(s) 362, 398, 540, 664
NlaIV GGNNCC 3 cut(s) 64, 253, 303
NmuCI GTSAC 1 cut(s) 616
NsiI ATGCAT 2 cut(s) 360, 456
PcsI WCGNNNNNNNCGW 3 cut(s) 120, 183, 275
PfeI GAWTC 2 cut(s) 266, 280
PkrI GCNGC 5 cut(s) 7, 16, 76, 205, 653
PleI GAGTC 2 cut(s) 188, 319
PpsI GAGTC 2 cut(s) 188, 319
PshBI ATTAAT 1 cut(s) 723
Psp6I CCWGG 2 cut(s) 69, 216
PspGI CCWGG 2 cut(s) 69, 216
PspN4I GGNNCC 3 cut(s) 64, 253, 303
PspPI GGNCC 5 cut(s) 20, 25, 63, 251, 508
PsuI RGATCY 1 cut(s) 301
RsaI GTAC 1 cut(s) 389
RsaNI GTAC 1 cut(s) 388
RseI CAYNNNNRTG 1 cut(s) 659
SaqAI TTAA 2 cut(s) 81, 723
SatI GCNGC 5 cut(s) 6, 15, 75, 204, 652
Sau3AI GATC 5 cut(s) 146, 189, 273, 301, 336
Sau96I GGNCC 5 cut(s) 20, 25, 63, 251, 508
SchI GAGTC 2 cut(s) 188, 319
ScrFI CCNGG 3 cut(s) 71, 218, 343
SfaNI GCATC 1 cut(s) 412
SfiI GGCCNNNNNGGCC 1 cut(s) 71
SinI GGWCC 3 cut(s) 20, 25, 508
SmiMI CAYNNNNRTG 1 cut(s) 659
SmlI CTYRAG 1 cut(s) 383
SmoI CTYRAG 1 cut(s) 383
Sse9I AATT 2 cut(s) 409, 724
SsiI CCGC 5 cut(s) 43, 75, 201, 204, 299
SspI AATATT 1 cut(s) 441
SspMI CTAG 1 cut(s) 11
StyD4I CCNGG 3 cut(s) 69, 216, 341
TaaI ACNGT 1 cut(s) 349
TaqI TCGA 3 cut(s) 123, 192, 278
TasI AATT 2 cut(s) 409, 724
TatI WGTACW 1 cut(s) 387
TauI GCSGC 2 cut(s) 77, 206
TfiI GAWTC 2 cut(s) 266, 280
Tru1I TTAA 2 cut(s) 81, 723
Tru9I TTAA 2 cut(s) 81, 723
TscAI CASTG 4 cut(s) 432, 558, 693, 708
TseFI GTSAC 1 cut(s) 616
TseI GCWGC 3 cut(s) 5, 14, 651
Tsp45I GTSAC 1 cut(s) 616
TspDTI ATGAA 2 cut(s) 390, 690
TspGWI ACGGA 1 cut(s) 651
TspRI CASTG 4 cut(s) 432, 558, 693, 708
VpaK11BI GGWCC 3 cut(s) 20, 25, 508
VspI ATTAAT 1 cut(s) 723
XspI CTAG 1 cut(s) 11
Zsp2I ATGCAT 2 cut(s) 360, 456
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.