Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb3

Orthogroup ID

OG0005973

Physical Location & Seq

Genomic Coordinates Reverse (-)

8974199 .. 8978578

Gene Structure 4380 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_3g14550.t5

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1209 bp

ATGGAGACGAGTCCGCACTCCAACTCCGGTGGAGACTGCCGGAGGGAACCTCTTCTCGACTCGGAAAACGGCGTCGCATCAAGCAGGTCTTGGAGGCTTGATATCAATGAGTTTCGGATCCCGGAACAGAACAACGGCGAAGGTGAAAAAGGGTGTCGACCCTTTGGTGGTATCCGTCGTCTATACACACCCAGGAAAAATTACAGAGTTGAGCAATATTACAAGCAACAAGGAAGGCTTCTTGAAGGGTACAGTGAGATGGAGACCATGACTGAAGAGGGTTGGTTGCCAGGAAGTCTCACAGAGGATGAAATGAAGCATCTAGCCAAGAGTGAGAGGATGGCTGTTCATATATCAAATATAACTAATTTGGTGCTCTTTGCTGCAAAAGTTTATGCCTCTATCATGAGCAGATCACTGGCAGTTATTGCCTCCACCTTGGATTCCCTCTTGGATCTCTTGTCTGGTTTTATTTTGTGGTTCACTGCCCATGCCATGAGAAAGCCAAACCGCTTAAATTACCCTATTGGAAAGAAACGTATGCAACCAGTGGGTATCATTGTCTTTGCGTCTGTTATGGCAACTCTTGGACTGCAAATATTGCTTGAGTCTGGTCGGGAACTGGTCTCAAAGTCCCGATCGGAAATGGGTGATGCACAGGAGAAGTGGATGATTGCCATCATGGTCAGTGTCACAGTTGTGAAGTTTATACTCATGATCTATTGTCGTAGATTCAAGAATGAAATTGTACGGGCCTATGCACAAGACCACTTCTTTGATGTCATTACCAATTCAGTTGGTCTAGCAGCAGCAGTCCTAGCTCACCGGTATGCATGGTGGATTGATCCTACAGGTGCTATTGTGATTGCACTTTATACCATCAATACATGGACAAGGACAGTCCTGGAGAACGTGCGAACGCTAATTGGAAGAACAGCTCCACCTGAGTTTTTGGCCAAGTTGACCTATCTGATATGGAATCACCACGAGGAGATCAAGCACATTGACACAGTGAGAGCATACACATTTGGTAACTACTACTTTGTCGAGGTCGACATAGTCTTGCCAGAAGACATGCTTCTGAACAAAGCTCATAACATTGGCGAGACACTCCAGGAGAAGCTCGAGCAACTTGCTGAAGTCGAACGGGCCTTTGTGCACATAGATTTTGAGTTCAGTCACCGGCCGGAACACAAAGCTACCGAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_efflux ZT_dimer

Protein Analysis

403

Amino Acids

46.06

Weight (kDa)

6.52

Isoelectric Point (pI)

46.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cation_efflux	PF01545	117 - 309	1.4e-32	Cation efflux transmembrane domain
ZT_dimer	PF16916	316 - 389	5.1e-09	Cation efflux protein, cytoplasmic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005973)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G16310 AT1G16310 AT1G79520 AT1G79520 AT1G79520 AT1G79520
fragaria_vesca	FvH4_3g14550 FvH4_3g14550 FvH4_3g14550 FvH4_3g14550 FvH4_3g14550
malus_domestica	MD05G1235400.v1.1 MD10G1211700.v1.1 MD10G1211900.v1.1
prunus_persica	Prupe.4G131000_v2.0.a1 Prupe.4G131000_v2.0.a1
pyrus_communis	pycom05g21230
rosa_chinensis	RchiOBHm_Chr5g0023931
rosa_laevigata	RLG00000032750
rosa_multiflora	Rmu_sc0004175.1_g000012 Rmu_sc0005837.1_g000003
rosa_roxburghii	Rroxscaffold_1G00055290
rosa_rugosa	Rorug05G0080400
rosa_samantha	Rh5AG170800 Rh5BG167800 Rh5CG183300
rosa_wichuraiana	Rw5G015440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	75
AccI	GTMKAC	2 cut(s)	157, 1053
AciI	CCGC	2 cut(s)	14, 511
AclWI	GGATC	4 cut(s)	112, 125, 462, 839
AcoI	YGGCCR	2 cut(s)	954, 1184
AcuI	CTGAAG	2 cut(s)	294, 1158
AcyI	GRCGYC	1 cut(s)	72
AfaI	GTAC	2 cut(s)	251, 750
AfiI	CCNNNNNNNGG	1 cut(s)	167
AgeI	ACCGGT	1 cut(s)	825
AgsI	TTSAA	2 cut(s)	245, 736
AjnI	CCWGG	4 cut(s)	191, 289, 903, 1113
AleI	CACNNNNGTG	1 cut(s)	698
AluBI	AGCT	5 cut(s)	821, 938, 1091, 1123, 1199
AluI	AGCT	5 cut(s)	821, 938, 1091, 1123, 1199
Alw21I	GWGCWC	2 cut(s)	378, 1161
Alw26I	GTCTC	5 cut(s)	27, 257, 302, 631, 1100
Alw44I	GTGCAC	1 cut(s)	1157
AlwI	GGATC	4 cut(s)	112, 125, 462, 839
Ama87I	CYCGRG	1 cut(s)	1124
AoxI	GGCC	4 cut(s)	753, 954, 1149, 1184
ApaLI	GTGCAC	1 cut(s)	1157
ApeKI	GCWGC	3 cut(s)	383, 806, 809
ArsI	GACNNNNNNTTYG	2 cut(s)	758, 790
AsiGI	ACCGGT	1 cut(s)	825
Asp700I	GAANNNNTTC	1 cut(s)	51
AspS9I	GGNCC	2 cut(s)	753, 1149
AsuC2I	CCSGG	1 cut(s)	122
AsuHPI	GGTGA	5 cut(s)	155, 662, 815, 974, 1172
AvaI	CYCGRG	1 cut(s)	1124
BaeGI	GKGCMC	1 cut(s)	1161
BalI	TGGCCA	1 cut(s)	956
BamHI	GGATCC	1 cut(s)	117
BauI	CACGAG	1 cut(s)	986
BbsI	GAAGAC	1 cut(s)	1077
Bbv12I	GWGCWC	2 cut(s)	378, 1161
BbvI	GCAGC	3 cut(s)	370, 818, 821
BccI	CCATC	4 cut(s)	253, 334, 686, 887
BceAI	ACGGC	2 cut(s)	85, 151
BcgI	CGANNNNNNTGC	2 cut(s)	1115, 1149
BciT130I	CCWGG	4 cut(s)	193, 291, 905, 1115
BciVI	GTATCC	1 cut(s)	182
BcnI	CCSGG	1 cut(s)	122
BcoDI	GTCTC	5 cut(s)	27, 257, 302, 631, 1100
BfaI	CTAG	3 cut(s)	323, 803, 818
BfmI	CTRYAG	1 cut(s)	849
BfuAI	ACCTGC	1 cut(s)	75
BfuI	GTATCC	1 cut(s)	182
BisI	GCNGC	3 cut(s)	384, 807, 810
BlsI	GCNGC	3 cut(s)	385, 808, 811
Bme1390I	CCNGG	5 cut(s)	122, 193, 291, 905, 1115
BmeT110I	CYCGRG	1 cut(s)	1124
BmgT120I	GGNCC	2 cut(s)	753, 1149
BmiI	GGNNCC	2 cut(s)	48, 119
BmrFI	CCNGG	5 cut(s)	122, 193, 291, 905, 1115
BmsI	GCATC	3 cut(s)	86, 328, 643
BpiI	GAAGAC	1 cut(s)	1077
BplI	GAGNNNNNCTC	2 cut(s)	34, 66
BpmI	CTGGAG	2 cut(s)	926, 1097
BpuEI	CTTGAG	1 cut(s)	626
BpuMI	CCSGG	1 cut(s)	122
BsaBI	GATNNNNATC	1 cut(s)	677
BsaHI	GRCGYC	1 cut(s)	72
BsaI	GGTCTC	2 cut(s)	257, 631
BsaJI	CCNNGG	2 cut(s)	191, 438
BsaWI	WCCGGW	2 cut(s)	26, 825
BsaXI	ACNNNNNCTCC	2 cut(s)	8, 38
Bsc4I	CCNNNNNNNGG	1 cut(s)	167
Bse118I	RCCGGY	2 cut(s)	825, 1182
Bse1I	ACTGG	3 cut(s)	423, 548, 627
Bse8I	GATNNNNATC	1 cut(s)	677
BseBI	CCWGG	4 cut(s)	193, 291, 905, 1115
BseDI	CCNNGG	2 cut(s)	191, 438
BseGI	GGATG	3 cut(s)	313, 345, 675
BseJI	GATNNNNATC	1 cut(s)	677
BseLI	CCNNNNNNNGG	1 cut(s)	167
BseMII	CTCAG	1 cut(s)	936
BseNI	ACTGG	3 cut(s)	423, 548, 627
BseRI	GAGGAG	1 cut(s)	1004
BseSI	GKGCMC	1 cut(s)	1161
BseX3I	CGGCCG	1 cut(s)	1184
BseXI	GCAGC	3 cut(s)	370, 818, 821
Bsh1285I	CGRYCG	2 cut(s)	641, 1187
BshFI	GGCC	4 cut(s)	755, 956, 1151, 1186
BshTI	ACCGGT	1 cut(s)	825
BsiEI	CGRYCG	2 cut(s)	641, 1187
BsiHKAI	GWGCWC	2 cut(s)	378, 1161
BsiHKCI	CYCGRG	1 cut(s)	1124
BsiSI	CCGG	6 cut(s)	27, 40, 122, 826, 1183, 1187
BslFI	GGGAC	1 cut(s)	619
BslI	CCNNNNNNNGG	1 cut(s)	167
BsmAI	GTCTC	5 cut(s)	27, 257, 302, 631, 1100
BsmFI	GGGAC	1 cut(s)	619
BsnI	GGCC	4 cut(s)	755, 956, 1151, 1186
Bso31I	GGTCTC	2 cut(s)	257, 631
BsoBI	CYCGRG	1 cut(s)	1124
Bsp1286I	GDGCHC	2 cut(s)	378, 1161
Bsp143I	GATC	7 cut(s)	117, 413, 454, 638, 717, 844, 993
BspACI	CCGC	2 cut(s)	14, 511
BspANI	GGCC	4 cut(s)	755, 956, 1151, 1186
BspCNI	CTCAG	1 cut(s)	937
BspHI	TCATGA	2 cut(s)	405, 714
BspLI	GGNNCC	2 cut(s)	48, 119
BspMI	ACCTGC	1 cut(s)	75
BspPI	GGATC	4 cut(s)	112, 125, 462, 839
BspTNI	GGTCTC	2 cut(s)	257, 631
BsrFI	RCCGGY	2 cut(s)	825, 1182
BsrI	ACTGG	3 cut(s)	423, 548, 627
BssAI	RCCGGY	2 cut(s)	825, 1182
BssECI	CCNNGG	2 cut(s)	191, 438
BssMI	GATC	7 cut(s)	117, 413, 454, 638, 717, 844, 993
BssNI	GRCGYC	1 cut(s)	72
BssSI	CACGAG	1 cut(s)	986
BssT1I	CCWWGG	1 cut(s)	438
Bst2BI	CACGAG	1 cut(s)	986
Bst2UI	CCWGG	4 cut(s)	193, 291, 905, 1115
Bst4CI	ACNGT	4 cut(s)	254, 697, 901, 1012
Bst6I	CTCTTC	2 cut(s)	57, 270
BstACI	GRCGYC	1 cut(s)	72
BstAPI	GCANNNNNTGC	2 cut(s)	428, 601
BstDEI	CTNAG	1 cut(s)	945
BstF5I	GGATG	3 cut(s)	313, 345, 675
BstKTI	GATC	7 cut(s)	120, 416, 457, 641, 720, 847, 996
BstMAI	GTCTC	5 cut(s)	27, 257, 302, 631, 1100
BstMBI	GATC	7 cut(s)	117, 413, 454, 638, 717, 844, 993
BstMCI	CGRYCG	2 cut(s)	641, 1187
BstMWI	GCNNNNNNNGC	3 cut(s)	428, 601, 818
BstNI	CCWGG	4 cut(s)	193, 291, 905, 1115
BstNSI	RCATGY	1 cut(s)	1078
BstSCI	CCNGG	5 cut(s)	120, 191, 289, 903, 1113
BstSFI	CTRYAG	1 cut(s)	849
BstSLI	GKGCMC	1 cut(s)	1161
BstV1I	GCAGC	3 cut(s)	370, 818, 821
BstV2I	GAAGAC	1 cut(s)	1077
BstX2I	RGATCY	2 cut(s)	117, 454
BstYI	RGATCY	2 cut(s)	117, 454
BstZI	CGGCCG	1 cut(s)	1184
BsuI	GTATCC	1 cut(s)	182
BsuRI	GGCC	4 cut(s)	755, 956, 1151, 1186
BtsCI	GGATG	3 cut(s)	313, 345, 675
BtsI	GCAGTG	1 cut(s)	483
BtsIMutI	CAGTG	6 cut(s)	259, 416, 483, 555, 694, 1017
BveI	ACCTGC	1 cut(s)	75
CciI	TCATGA	2 cut(s)	405, 714
Cfr10I	RCCGGY	2 cut(s)	825, 1182
Cfr13I	GGNCC	2 cut(s)	753, 1149
CseI	GACGC	2 cut(s)	61, 558
Csp6I	GTAC	2 cut(s)	250, 749
CspAI	ACCGGT	1 cut(s)	825
CspCI	CAANNNNNGTGG	2 cut(s)	10, 45
CviAII	CATG	9 cut(s)	268, 406, 491, 496, 682, 715, 834, 888, 1075
CviQI	GTAC	2 cut(s)	250, 749
DdeI	CTNAG	1 cut(s)	945
DpnI	GATC	7 cut(s)	119, 415, 456, 640, 719, 846, 995
DpnII	GATC	7 cut(s)	117, 413, 454, 638, 717, 844, 993
EaeI	YGGCCR	2 cut(s)	954, 1184
EagI	CGGCCG	1 cut(s)	1184
Eam1104I	CTCTTC	2 cut(s)	57, 270
EarI	CTCTTC	2 cut(s)	57, 270
EclXI	CGGCCG	1 cut(s)	1184
Eco130I	CCWWGG	1 cut(s)	438
Eco31I	GGTCTC	2 cut(s)	257, 631
Eco32I	GATATC	1 cut(s)	103
Eco52I	CGGCCG	1 cut(s)	1184
Eco57I	CTGAAG	2 cut(s)	294, 1158
Eco88I	CYCGRG	1 cut(s)	1124
EcoRII	CCWGG	4 cut(s)	191, 289, 903, 1113
EcoRV	GATATC	1 cut(s)	103
EcoT14I	CCWWGG	1 cut(s)	438
EcoT22I	ATGCAT	1 cut(s)	835
ErhI	CCWWGG	1 cut(s)	438
FaeI	CATG	9 cut(s)	271, 409, 494, 499, 685, 718, 837, 891, 1078
FalI	AAGNNNNNCTT	6 cut(s)	73, 105, 222, 254, 1062, 1094
FaqI	GGGAC	1 cut(s)	619
FatI	CATG	9 cut(s)	267, 405, 490, 495, 681, 714, 833, 887, 1074
FblI	GTMKAC	2 cut(s)	157, 1053
Fnu4HI	GCNGC	3 cut(s)	384, 807, 810
FokI	GGATG	3 cut(s)	320, 352, 682
Fsp4HI	GCNGC	3 cut(s)	384, 807, 810
FspBI	CTAG	3 cut(s)	323, 803, 818
GluI	GCNGC	3 cut(s)	384, 807, 810
GsuI	CTGGAG	2 cut(s)	926, 1097
HaeIII	GGCC	4 cut(s)	755, 956, 1151, 1186
HapII	CCGG	6 cut(s)	27, 40, 122, 826, 1183, 1187
HgaI	GACGC	2 cut(s)	61, 558
Hin1I	GRCGYC	1 cut(s)	72
Hin1II	CATG	9 cut(s)	271, 409, 494, 499, 685, 718, 837, 891, 1078
HincII	GTYRAC	3 cut(s)	158, 963, 1054
HindII	GTYRAC	3 cut(s)	158, 963, 1054
HinfI	GANTC	6 cut(s)	10, 59, 443, 608, 732, 979
HpaII	CCGG	6 cut(s)	27, 40, 122, 826, 1183, 1187
HphI	GGTGA	5 cut(s)	155, 662, 815, 974, 1172
Hpy166II	GTNNAC	5 cut(s)	158, 483, 963, 1054, 1159
Hpy188I	TCNGA	5 cut(s)	64, 117, 643, 972, 1083
Hpy188III	TCNNGA	7 cut(s)	56, 242, 406, 617, 636, 715, 736
Hpy8I	GTNNAC	5 cut(s)	158, 483, 963, 1054, 1159
Hpy99I	CGWCG	2 cut(s)	77, 180
HpyAV	CCTTC	3 cut(s)	134, 228, 239
HpyCH4III	ACNGT	4 cut(s)	254, 697, 901, 1012
HpyCH4IV	ACGT	2 cut(s)	538, 912
HpyCH4V	TGCA	8 cut(s)	386, 544, 595, 656, 761, 833, 869, 1159
HpyF10VI	GCNNNNNNNGC	3 cut(s)	428, 601, 818
HpyF3I	CTNAG	1 cut(s)	945
HpySE526I	ACGT	2 cut(s)	538, 912
Hsp92I	GRCGYC	1 cut(s)	72
Hsp92II	CATG	9 cut(s)	271, 409, 494, 499, 685, 718, 837, 891, 1078
Kzo9I	GATC	7 cut(s)	117, 413, 454, 638, 717, 844, 993
LmnI	GCTCC	1 cut(s)	943
Lsp1109I	GCAGC	3 cut(s)	370, 818, 821
LweI	GCATC	3 cut(s)	86, 328, 643
MaeI	CTAG	3 cut(s)	323, 803, 818
MaeII	ACGT	2 cut(s)	538, 912
MaeIII	GTNAC	3 cut(s)	691, 1031, 1178
MalI	GATC	7 cut(s)	119, 415, 456, 640, 719, 846, 995
MboI	GATC	7 cut(s)	117, 413, 454, 638, 717, 844, 993
MboII	GAAGA	4 cut(s)	44, 287, 942, 1082
MflI	RGATCY	2 cut(s)	117, 454
MhlI	GDGCHC	2 cut(s)	378, 1161
MlsI	TGGCCA	1 cut(s)	956
MluCI	AATT	6 cut(s)	199, 367, 517, 744, 790, 924
MluNI	TGGCCA	1 cut(s)	956
MlyI	GAGTC	3 cut(s)	19, 53, 617
MmeI	TCCRAC	1 cut(s)	45
Mox20I	TGGCCA	1 cut(s)	956
Mph1103I	ATGCAT	1 cut(s)	835
MroXI	GAANNNNTTC	1 cut(s)	51
MscI	TGGCCA	1 cut(s)	956
MseI	TTAA	1 cut(s)	515
MslI	CAYNNNNRTG	2 cut(s)	698, 828
Msp20I	TGGCCA	1 cut(s)	956
MspI	CCGG	6 cut(s)	27, 40, 122, 826, 1183, 1187
MspR9I	CCNGG	5 cut(s)	122, 193, 291, 905, 1115
MvaI	CCWGG	4 cut(s)	193, 291, 905, 1115
MwoI	GCNNNNNNNGC	3 cut(s)	428, 601, 818
NciI	CCSGG	1 cut(s)	122
NdeII	GATC	7 cut(s)	117, 413, 454, 638, 717, 844, 993
NlaIII	CATG	9 cut(s)	271, 409, 494, 499, 685, 718, 837, 891, 1078
NlaIV	GGNNCC	2 cut(s)	48, 119
NmuCI	GTSAC	2 cut(s)	691, 1178
NsiI	ATGCAT	1 cut(s)	835
NspI	RCATGY	1 cut(s)	1078
OliI	CACNNNNGTG	1 cut(s)	698
PaeR7I	CTCGAG	1 cut(s)	1124
PagI	TCATGA	2 cut(s)	405, 714
PdmI	GAANNNNTTC	1 cut(s)	51
PfeI	GAWTC	3 cut(s)	443, 732, 979
PflFI	GACNNNGTC	1 cut(s)	1058
PfoI	TCCNGGA	3 cut(s)	120, 903, 1113
PinAI	ACCGGT	1 cut(s)	825
PkrI	GCNGC	3 cut(s)	385, 808, 811
Ple19I	CGATCG	1 cut(s)	641
PleI	GAGTC	3 cut(s)	18, 53, 616
PpsI	GAGTC	3 cut(s)	18, 53, 616
Psp6I	CCWGG	4 cut(s)	191, 289, 903, 1113
PspGI	CCWGG	4 cut(s)	191, 289, 903, 1113
PspN4I	GGNNCC	2 cut(s)	48, 119
PspPI	GGNCC	2 cut(s)	753, 1149
PspXI	VCTCGAGB	1 cut(s)	1124
PsuI	RGATCY	2 cut(s)	117, 454
PsyI	GACNNNGTC	1 cut(s)	1058
PvuI	CGATCG	1 cut(s)	641
RsaI	GTAC	2 cut(s)	251, 750
RsaNI	GTAC	2 cut(s)	250, 749
RseI	CAYNNNNRTG	2 cut(s)	698, 828
SalI	GTCGAC	2 cut(s)	156, 1052
SaqAI	TTAA	1 cut(s)	515
SatI	GCNGC	3 cut(s)	384, 807, 810
Sau3AI	GATC	7 cut(s)	117, 413, 454, 638, 717, 844, 993
Sau96I	GGNCC	2 cut(s)	753, 1149
SchI	GAGTC	3 cut(s)	19, 53, 617
ScrFI	CCNGG	5 cut(s)	122, 193, 291, 905, 1115
SduI	GDGCHC	2 cut(s)	378, 1161
SfaNI	GCATC	3 cut(s)	86, 328, 643
SfcI	CTRYAG	1 cut(s)	849
Sfr274I	CTCGAG	1 cut(s)	1124
SlaI	CTCGAG	1 cut(s)	1124
SmiMI	CAYNNNNRTG	2 cut(s)	698, 828
SmlI	CTYRAG	2 cut(s)	605, 1124
SmoI	CTYRAG	2 cut(s)	605, 1124
Sse9I	AATT	6 cut(s)	199, 367, 517, 744, 790, 924
SsiI	CCGC	2 cut(s)	14, 511
SspI	AATATT	2 cut(s)	218, 600
SspMI	CTAG	3 cut(s)	323, 803, 818
StyD4I	CCNGG	5 cut(s)	120, 191, 289, 903, 1113
StyI	CCWWGG	1 cut(s)	438
TaaI	ACNGT	4 cut(s)	254, 697, 901, 1012
TaiI	ACGT	2 cut(s)	541, 915
TaqI	TCGA	6 cut(s)	57, 157, 1047, 1053, 1125, 1143
TasI	AATT	6 cut(s)	199, 367, 517, 744, 790, 924
TfiI	GAWTC	3 cut(s)	443, 732, 979
Tru1I	TTAA	1 cut(s)	515
Tru9I	TTAA	1 cut(s)	515
TscAI	CASTG	6 cut(s)	259, 423, 490, 555, 694, 1017
TseFI	GTSAC	2 cut(s)	691, 1178
TseI	GCWGC	3 cut(s)	383, 806, 809
Tsp45I	GTSAC	2 cut(s)	691, 1178
TspDTI	ATGAA	4 cut(s)	324, 329, 338, 756
TspGWI	ACGGA	1 cut(s)	164
TspRI	CASTG	6 cut(s)	259, 423, 490, 555, 694, 1017
Tth111I	GACNNNGTC	1 cut(s)	1058
VneI	GTGCAC	1 cut(s)	1157
XceI	RCATGY	1 cut(s)	1078
XhoI	CTCGAG	1 cut(s)	1124
XmiI	GTMKAC	2 cut(s)	157, 1053
XmnI	GAANNNNTTC	1 cut(s)	51
XspI	CTAG	3 cut(s)	323, 803, 818
Zsp2I	ATGCAT	1 cut(s)	835

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.