Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0005973

Physical Location & Seq

Genomic Coordinates Forward (+)

31086146 .. 31088747

Gene Structure 2602 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1211900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1071 bp

ATGGTTTTATCAATAATGATAAATTTAGGACACTTTTGGCTTTTAAACTCAGGGAAAGAGTTCAAGGTTGAAGAATATTACAAGCAACAAGAAAGGCTTCTTGAAGGGTTCAGTGAGATGGAGACCATGACTGAAGAGGGTTGGTTGCCCGCAAGTCTTACAGAGGATCAAATGAAGCAGCTAGCGAAGAGGGAGCGGATGGCAGTTCATGCATCAAACGTAGCTAATTTGCTTCTTTTTGCCGCAAAAGTTTATGCTTCAATTATGAGCAGATCATTGGCAGTTATTGCCTCTACCTTGGATTCCCTCTTGGATCTCTTATCTGGCTTTATTTTGTGGTTCACTGCTAATGCCATGAAAAATTCCAACCAGTTTCGCTATCCGATTGGGAAGAAACGTATGCAACCAGTGGGTATCATTGTCTTTGCATCTGTTATGGCTACTCTTGGACTGCAAATATTGATTGAGTGTGGTCGGGAACTCATCTCACAGTCACCGCCTGCAAAAATGAATCGTACGCAGGAGAATTGGATGATCGGCATCATGGTCTCAGTCACGGTAGTGAAGTTTGTGCTCATGGTCTACTGTCGTAGATTTAAGAATGAAATTGTACGGGCCTATGCACAAGACCATTTTTTCGATGTCATTACTAATTCAGTTGGTTTAGCAGCAGCAGTCCTAGCTGTCCGGTTCTTCTGGTGGATTGATCCTACTGGTGCTATTATAATAGCACTATATACCATTAATACGTGGACAAAGACAGTCTTGGAGAATGTACATGCGCTAATTGGAAGAACAGCACCACCTGACTTTTTGGCAAAGTTGACATACTTGATATGGAACCACCACGAAGAGATCGAGCACATTGATACAGTGAGGGCGTACACATTTGGTTCCCATTACTTTGTGGAGGTTGACATTGTCTTGCCAGAAGACATGCTACTGAACAAAGCGCACAACATTGGCGAGACTCTCCAAGAGAAGCTTGAGCAACTCCCTGAAGTGGAACGGGCTTTTGTGCATATAGATTTTGAGTTCACTCACAGGCCGGAACACAAGAACAGTGTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_efflux ZT_dimer

Protein Analysis

357

Amino Acids

40.83

Weight (kDa)

6.07

Isoelectric Point (pI)

47.57

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cation_efflux	PF01545	70 - 262	1.9e-30	Cation efflux transmembrane domain
ZT_dimer	PF16916	277 - 343	1.4e-08	Cation efflux protein, cytoplasmic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0005973)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G16310 AT1G16310 AT1G79520 AT1G79520 AT1G79520 AT1G79520
fragaria_vesca	FvH4_3g14550 FvH4_3g14550 FvH4_3g14550 FvH4_3g14550 FvH4_3g14550
malus_domestica	MD05G1235400.v1.1 MD10G1211700.v1.1 MD10G1211900.v1.1
prunus_persica	Prupe.4G131000_v2.0.a1 Prupe.4G131000_v2.0.a1
pyrus_communis	pycom05g21230
rosa_chinensis	RchiOBHm_Chr5g0023931
rosa_laevigata	RLG00000032750
rosa_multiflora	Rmu_sc0004175.1_g000012 Rmu_sc0005837.1_g000003
rosa_roxburghii	Rroxscaffold_1G00055290
rosa_rugosa	Rorug05G0080400
rosa_samantha	Rh5AG170800 Rh5BG167800 Rh5CG183300
rosa_wichuraiana	Rw5G015440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	725
AccBSI	CCGCTC	1 cut(s)	196
AccI	GTMKAC	1 cut(s)	582
AciI	CCGC	4 cut(s)	150, 196, 243, 497
AclWI	GGATC	3 cut(s)	174, 321, 701
AcsI	RAATTY	2 cut(s)	22, 361
AcuI	CTGAAG	2 cut(s)	153, 1020
AfaI	GTAC	4 cut(s)	517, 612, 777, 884
AfiI	CCNNNNNNNGG	1 cut(s)	1003
AgsI	TTSAA	4 cut(s)	64, 71, 104, 261
AleI	CACNNNNGTG	1 cut(s)	560
AluBI	AGCT	4 cut(s)	181, 224, 683, 985
AluI	AGCT	4 cut(s)	181, 224, 683, 985
Alw21I	GWGCWC	2 cut(s)	576, 864
Alw26I	GTCTC	3 cut(s)	116, 553, 962
AlwI	GGATC	3 cut(s)	174, 321, 701
AoxI	GGCC	2 cut(s)	615, 1046
ApeKI	GCWGC	3 cut(s)	178, 668, 671
ApoI	RAATTY	2 cut(s)	22, 361
ArsI	GACNNNNNNTTYG	2 cut(s)	620, 652
AseI	ATTAAT	1 cut(s)	744
Asp700I	GAANNNNTTC	2 cut(s)	59, 96
AspLEI	GCGC	2 cut(s)	784, 955
AspS9I	GGNCC	1 cut(s)	615
AsuHPI	GGTGA	1 cut(s)	486
AsuNHI	GCTAGC	1 cut(s)	181
BarI	GAAGNNNNNNTAC	2 cut(s)	924, 956
BbsI	GAAGAC	1 cut(s)	939
Bbv12I	GWGCWC	2 cut(s)	576, 864
BbvI	GCAGC	3 cut(s)	190, 680, 683
BccI	CCATC	2 cut(s)	112, 193
BcoDI	GTCTC	3 cut(s)	116, 553, 962
BfaI	CTAG	2 cut(s)	182, 680
BisI	GCNGC	4 cut(s)	179, 243, 669, 672
BlsI	GCNGC	4 cut(s)	180, 244, 670, 673
BmgT120I	GGNCC	1 cut(s)	615
BmiI	GGNNCC	2 cut(s)	842, 895
BmsI	GCATC	3 cut(s)	221, 437, 549
BmtI	GCTAGC	1 cut(s)	185
BpiI	GAAGAC	1 cut(s)	939
BpuEI	CTTGAG	1 cut(s)	1007
BsaAI	YACGTR	1 cut(s)	750
BsaBI	GATNNNNATC	1 cut(s)	539
BsaI	GGTCTC	2 cut(s)	116, 553
BsaJI	CCNNGG	1 cut(s)	297
BsaWI	WCCGGW	1 cut(s)	687
Bsc4I	CCNNNNNNNGG	1 cut(s)	1003
Bse1I	ACTGG	3 cut(s)	370, 407, 718
Bse8I	GATNNNNATC	1 cut(s)	539
BseDI	CCNNGG	1 cut(s)	297
BseGI	GGATG	2 cut(s)	204, 537
BseJI	GATNNNNATC	1 cut(s)	539
BseLI	CCNNNNNNNGG	1 cut(s)	1003
BseMII	CTCAG	2 cut(s)	63, 564
BseNI	ACTGG	3 cut(s)	370, 407, 718
BseXI	GCAGC	3 cut(s)	190, 680, 683
BshFI	GGCC	2 cut(s)	617, 1048
BsiHKAI	GWGCWC	2 cut(s)	576, 864
BsiSI	CCGG	2 cut(s)	688, 1049
BsiWI	CGTACG	1 cut(s)	515
BslI	CCNNNNNNNGG	1 cut(s)	1003
BsmAI	GTCTC	3 cut(s)	116, 553, 962
BsnI	GGCC	2 cut(s)	617, 1048
Bso31I	GGTCTC	2 cut(s)	116, 553
Bsp1286I	GDGCHC	2 cut(s)	576, 864
Bsp1407I	TGTACA	1 cut(s)	775
Bsp143I	GATC	6 cut(s)	166, 272, 313, 534, 706, 855
BspACI	CCGC	4 cut(s)	150, 196, 243, 497
BspANI	GGCC	2 cut(s)	617, 1048
BspCNI	CTCAG	2 cut(s)	62, 563
BspLI	GGNNCC	2 cut(s)	842, 895
BspOI	GCTAGC	1 cut(s)	185
BspPI	GGATC	3 cut(s)	174, 321, 701
BspTNI	GGTCTC	2 cut(s)	116, 553
BsrBI	CCGCTC	1 cut(s)	196
BsrGI	TGTACA	1 cut(s)	775
BsrI	ACTGG	3 cut(s)	370, 407, 718
BssECI	CCNNGG	1 cut(s)	297
BssMI	GATC	6 cut(s)	166, 272, 313, 534, 706, 855
BssT1I	CCWWGG	1 cut(s)	297
Bst4CI	ACNGT	6 cut(s)	492, 559, 587, 763, 874, 1064
Bst6I	CTCTTC	3 cut(s)	129, 182, 846
BstAPI	GCANNNNNTGC	2 cut(s)	209, 287
BstAUI	TGTACA	1 cut(s)	775
BstBAI	YACGTR	1 cut(s)	750
BstC8I	GCNNGC	3 cut(s)	150, 183, 501
BstDEI	CTNAG	2 cut(s)	49, 550
BstF5I	GGATG	2 cut(s)	204, 537
BstHHI	GCGC	2 cut(s)	784, 955
BstKTI	GATC	6 cut(s)	169, 275, 316, 537, 709, 858
BstMAI	GTCTC	3 cut(s)	116, 553, 962
BstMBI	GATC	6 cut(s)	166, 272, 313, 534, 706, 855
BstMWI	GCNNNNNNNGC	3 cut(s)	209, 287, 680
BstNSI	RCATGY	2 cut(s)	782, 940
BstV1I	GCAGC	3 cut(s)	190, 680, 683
BstV2I	GAAGAC	1 cut(s)	939
BstX2I	RGATCY	1 cut(s)	313
BstYI	RGATCY	1 cut(s)	313
BsuRI	GGCC	2 cut(s)	617, 1048
BtsCI	GGATG	2 cut(s)	204, 537
BtsI	GCAGTG	1 cut(s)	342
BtsIMutI	CAGTG	5 cut(s)	118, 342, 414, 879, 1069
Cac8I	GCNNGC	3 cut(s)	150, 183, 501
CfoI	GCGC	2 cut(s)	784, 955
Cfr13I	GGNCC	1 cut(s)	615
Csp6I	GTAC	4 cut(s)	516, 611, 776, 883
CviAII	CATG	7 cut(s)	127, 209, 355, 544, 577, 779, 937
CviQI	GTAC	4 cut(s)	516, 611, 776, 883
DdeI	CTNAG	2 cut(s)	49, 550
DpnI	GATC	6 cut(s)	168, 274, 315, 536, 708, 857
DpnII	GATC	6 cut(s)	166, 272, 313, 534, 706, 855
DraI	TTTAAA	1 cut(s)	45
Eam1104I	CTCTTC	3 cut(s)	129, 182, 846
EarI	CTCTTC	3 cut(s)	129, 182, 846
Eco130I	CCWWGG	1 cut(s)	297
Eco31I	GGTCTC	2 cut(s)	116, 553
Eco57I	CTGAAG	2 cut(s)	153, 1020
EcoT14I	CCWWGG	1 cut(s)	297
EcoT22I	ATGCAT	1 cut(s)	214
ErhI	CCWWGG	1 cut(s)	297
FaeI	CATG	7 cut(s)	130, 212, 358, 547, 580, 782, 940
FalI	AAGNNNNNCTT	6 cut(s)	81, 113, 749, 781, 969, 1001
FatI	CATG	7 cut(s)	126, 208, 354, 543, 576, 778, 936
FauI	CCCGC	1 cut(s)	157
FblI	GTMKAC	1 cut(s)	582
Fnu4HI	GCNGC	4 cut(s)	179, 243, 669, 672
FokI	GGATG	2 cut(s)	211, 544
Fsp4HI	GCNGC	4 cut(s)	179, 243, 669, 672
FspBI	CTAG	2 cut(s)	182, 680
GlaI	GCGC	2 cut(s)	783, 954
GluI	GCNGC	4 cut(s)	179, 243, 669, 672
HaeIII	GGCC	2 cut(s)	617, 1048
HapII	CCGG	2 cut(s)	688, 1049
HhaI	GCGC	2 cut(s)	784, 955
Hin1II	CATG	7 cut(s)	130, 212, 358, 547, 580, 782, 940
Hin6I	GCGC	2 cut(s)	782, 953
HinP1I	GCGC	2 cut(s)	782, 953
HincII	GTYRAC	2 cut(s)	825, 916
HindII	GTYRAC	2 cut(s)	825, 916
HindIII	AAGCTT	1 cut(s)	983
HinfI	GANTC	3 cut(s)	302, 511, 970
HpaII	CCGG	2 cut(s)	688, 1049
HphI	GGTGA	1 cut(s)	486
Hpy166II	GTNNAC	7 cut(s)	342, 583, 753, 825, 885, 916, 1038
Hpy188I	TCNGA	1 cut(s)	384
Hpy188III	TCNNGA	2 cut(s)	101, 476
Hpy8I	GTNNAC	7 cut(s)	342, 583, 753, 825, 885, 916, 1038
HpyAV	CCTTC	1 cut(s)	98
HpyCH4III	ACNGT	6 cut(s)	492, 559, 587, 763, 874, 1064
HpyCH4IV	ACGT	3 cut(s)	219, 397, 749
HpyCH4V	TGCA	7 cut(s)	212, 403, 428, 454, 503, 623, 1021
HpyF10VI	GCNNNNNNNGC	3 cut(s)	209, 287, 680
HpyF3I	CTNAG	2 cut(s)	49, 550
HpySE526I	ACGT	3 cut(s)	219, 397, 749
Hsp92II	CATG	7 cut(s)	130, 212, 358, 547, 580, 782, 940
HspAI	GCGC	2 cut(s)	782, 953
Kzo9I	GATC	6 cut(s)	166, 272, 313, 534, 706, 855
LmnI	GCTCC	1 cut(s)	193
Lsp1109I	GCAGC	3 cut(s)	190, 680, 683
LweI	GCATC	3 cut(s)	221, 437, 549
MaeI	CTAG	2 cut(s)	182, 680
MaeII	ACGT	3 cut(s)	219, 397, 749
MaeIII	GTNAC	2 cut(s)	492, 553
MalI	GATC	6 cut(s)	168, 274, 315, 536, 708, 857
MbiI	CCGCTC	1 cut(s)	196
MboI	GATC	6 cut(s)	166, 272, 313, 534, 706, 855
MboII	GAAGA	8 cut(s)	83, 146, 199, 403, 685, 804, 863, 944
MflI	RGATCY	1 cut(s)	313
MhlI	GDGCHC	2 cut(s)	576, 864
MluCI	AATT	8 cut(s)	22, 226, 261, 361, 526, 606, 652, 786
MlyI	GAGTC	1 cut(s)	964
MmeI	TCCRAC	1 cut(s)	390
MnlI	CCTC	7 cut(s)	130, 157, 183, 301, 317, 870, 904
Mph1103I	ATGCAT	1 cut(s)	214
MroXI	GAANNNNTTC	2 cut(s)	59, 96
MseI	TTAA	3 cut(s)	44, 597, 744
MslI	CAYNNNNRTG	1 cut(s)	560
MspI	CCGG	2 cut(s)	688, 1049
MwoI	GCNNNNNNNGC	3 cut(s)	209, 287, 680
NdeII	GATC	6 cut(s)	166, 272, 313, 534, 706, 855
NheI	GCTAGC	1 cut(s)	181
NlaIII	CATG	7 cut(s)	130, 212, 358, 547, 580, 782, 940
NlaIV	GGNNCC	2 cut(s)	842, 895
NmuCI	GTSAC	2 cut(s)	492, 553
NsiI	ATGCAT	1 cut(s)	214
NspI	RCATGY	2 cut(s)	782, 940
OliI	CACNNNNGTG	1 cut(s)	560
PcsI	WCGNNNNNNNCGW	1 cut(s)	855
PdmI	GAANNNNTTC	2 cut(s)	59, 96
PfeI	GAWTC	2 cut(s)	302, 511
Pfl23II	CGTACG	1 cut(s)	515
PflFI	GACNNNGTC	1 cut(s)	920
PkrI	GCNGC	4 cut(s)	180, 244, 670, 673
PleI	GAGTC	1 cut(s)	964
PpsI	GAGTC	1 cut(s)	964
Ppu21I	YACGTR	1 cut(s)	750
PshBI	ATTAAT	1 cut(s)	744
PsiI	TTATAA	1 cut(s)	725
PspLI	CGTACG	1 cut(s)	515
PspN4I	GGNNCC	2 cut(s)	842, 895
PspPI	GGNCC	1 cut(s)	615
PsuI	RGATCY	1 cut(s)	313
PsyI	GACNNNGTC	1 cut(s)	920
RsaI	GTAC	4 cut(s)	517, 612, 777, 884
RsaNI	GTAC	4 cut(s)	516, 611, 776, 883
RseI	CAYNNNNRTG	1 cut(s)	560
SaqAI	TTAA	3 cut(s)	44, 597, 744
SatI	GCNGC	4 cut(s)	179, 243, 669, 672
Sau3AI	GATC	6 cut(s)	166, 272, 313, 534, 706, 855
Sau96I	GGNCC	1 cut(s)	615
SchI	GAGTC	1 cut(s)	964
SduI	GDGCHC	2 cut(s)	576, 864
SfaNI	GCATC	3 cut(s)	221, 437, 549
SmiMI	CAYNNNNRTG	1 cut(s)	560
SmlI	CTYRAG	1 cut(s)	986
SmoI	CTYRAG	1 cut(s)	986
Sse9I	AATT	8 cut(s)	22, 226, 261, 361, 526, 606, 652, 786
SsiI	CCGC	4 cut(s)	150, 196, 243, 497
SspI	AATATT	2 cut(s)	77, 459
SspMI	CTAG	2 cut(s)	182, 680
StyI	CCWWGG	1 cut(s)	297
TaaI	ACNGT	6 cut(s)	492, 559, 587, 763, 874, 1064
TaiI	ACGT	3 cut(s)	222, 400, 752
TaqI	TCGA	2 cut(s)	639, 858
TasI	AATT	8 cut(s)	22, 226, 261, 361, 526, 606, 652, 786
TatI	WGTACW	1 cut(s)	775
TauI	GCSGC	1 cut(s)	245
TfiI	GAWTC	2 cut(s)	302, 511
Tru1I	TTAA	3 cut(s)	44, 597, 744
Tru9I	TTAA	3 cut(s)	44, 597, 744
TscAI	CASTG	5 cut(s)	118, 349, 414, 879, 1069
TseFI	GTSAC	2 cut(s)	492, 553
TseI	GCWGC	3 cut(s)	178, 668, 671
Tsp45I	GTSAC	2 cut(s)	492, 553
TspDTI	ATGAA	5 cut(s)	188, 197, 371, 524, 618
TspRI	CASTG	5 cut(s)	118, 349, 414, 879, 1069
Tth111I	GACNNNGTC	1 cut(s)	920
VspI	ATTAAT	1 cut(s)	744
XapI	RAATTY	2 cut(s)	22, 361
XceI	RCATGY	2 cut(s)	782, 940
XmiI	GTMKAC	1 cut(s)	582
XmnI	GAANNNNTTC	2 cut(s)	59, 96
XspI	CTAG	2 cut(s)	182, 680
Zsp2I	ATGCAT	1 cut(s)	214

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.