pycom05g21230

Metal tolerance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0005973
Physical Location & Seq
Forward (+)
23897699 .. 23899555
1857 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g21230.4

Sequence Viewer

Length: 981 bp
ATGTTAAGGAGTTCCGGCTTCCCCAGCAGAGCAGTACTACTGGTGACGACGATGACAGTTGACGCTGTCGTTTCGCTCTCGGTCGCCTCTGCGCGCCCAGATCAAATGAAGCAGCTGGCGAAGAGTGAGCGGATGGCAGTTCATGCATCAAACGTACTTAATTTGGTGCTTTTTGCTGCAAAAGTTTATGCTTCAATTACGAGCAGATCATTGTCAGTTATCGCCTCTACCTTGGATTCCTTGATCGATCTCTTATCCGTCTTTATTTTGTGGTTCACTGCTAATGCCATGAAAAATCCCAACCAGTTTCGCTATCCTATTGGGAAGAAGCGTATGCAGCCAGTGGTAAGGTATCATTGTCTTTGCATCTGTTATGGCTACTCTTGGACAAAAATATTGCTCGAGTCTGGTCGACAACTCGCGTCAAAGTCACCACCTGAAAAAATGAATAGTATGCAGGAGAAGTGGATGATCGGCATCATGGTCTCAGTCAAGGTGGTGAAGTTTGTGCTCATGGTCTACCGTCGTAGATTTAAAAATGAAATTGTACGGGGCTTATGCACAAGACCATTTTTTCGATGTCCTTACCAATTCAGTTGGTTTAGCAGCAGCAGTCCTAGCTGTTTGGTTCTTCTGATAGCACTATACACGATTAATACATGGGCAAAGACAGTCTTGGAAAATGTACATGCGCTAATTGGACGAACAGCCCCACCCGAATTTTTAGCGAAGTTGACATACTTGATATGGAACCACCACGAAGAGATCAAGCACATTGACACAGTGAGGGCGTACACATTTGGTTCTCATTACTTTGTGGAGGTTGACATAGTCTTACCAGAAAACTTGCTTCTGAACAAAGCGCATAACATTGGTGAGACACTCCAAGAGAAGCTCGAGCAACTCCCAGAAGTCGAACGGGCTTTTGTGCATATAGATTTTGAGTTCACTCACAGGCCGGAACACAAGAACAGTGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cation_efflux ZT_dimer

Protein Analysis

327

Amino Acids

37.36

Weight (kDa)

9.51

Isoelectric Point (pI)

48.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 130
AccI GTMKAC 2 cut(s) 412, 519
AccII CGCG 2 cut(s) 94, 422
AciI CCGC 1 cut(s) 130
AcsI RAATTY 1 cut(s) 719
AfaI GTAC 5 cut(s) 36, 156, 549, 687, 794
AgsI TTSAA 1 cut(s) 195
AluBI AGCT 3 cut(s) 115, 621, 895
AluI AGCT 3 cut(s) 115, 621, 895
Alw21I GWGCWC 1 cut(s) 513
Alw26I GTCTC 2 cut(s) 490, 872
Ama87I CYCGRG 2 cut(s) 401, 896
AoxI GGCC 1 cut(s) 956
ApeKI GCWGC 5 cut(s) 112, 176, 337, 606, 609
ApoI RAATTY 1 cut(s) 719
ArsI GACNNNNNNTTYG 2 cut(s) 558, 590
AseI ATTAAT 1 cut(s) 654
AspLEI GCGC 4 cut(s) 94, 96, 694, 865
AsuHPI GGTGA 4 cut(s) 55, 423, 511, 887
AvaI CYCGRG 2 cut(s) 401, 896
BarI GAAGNNNNNNTAC 2 cut(s) 722, 754
Bbv12I GWGCWC 1 cut(s) 513
BbvI GCAGC 5 cut(s) 124, 163, 349, 618, 621
BccI CCATC 1 cut(s) 127
BcoDI GTCTC 2 cut(s) 490, 872
BfaI CTAG 1 cut(s) 618
BisI GCNGC 5 cut(s) 113, 177, 338, 607, 610
BlsI GCNGC 5 cut(s) 114, 178, 339, 608, 611
BmcAI AGTACT 1 cut(s) 36
BmeT110I CYCGRG 2 cut(s) 401, 896
BmiI GGNNCC 1 cut(s) 752
BmsI GCATC 3 cut(s) 155, 375, 486
Bsa29I ATCGAT 1 cut(s) 246
BsaBI GATNNNNATC 1 cut(s) 476
BsaI GGTCTC 1 cut(s) 490
BsaJI CCNNGG 1 cut(s) 231
Bse1I ACTGG 3 cut(s) 45, 304, 341
Bse8I GATNNNNATC 1 cut(s) 476
BseCI ATCGAT 1 cut(s) 246
BseDI CCNNGG 1 cut(s) 231
BseGI GGATG 2 cut(s) 138, 474
BseJI GATNNNNATC 1 cut(s) 476
BseMII CTCAG 1 cut(s) 501
BseNI ACTGG 3 cut(s) 45, 304, 341
BsePI GCGCGC 1 cut(s) 92
BseXI GCAGC 5 cut(s) 124, 163, 349, 618, 621
BseYI CCCAGC 1 cut(s) 23
Bsh1236I CGCG 2 cut(s) 94, 422
Bsh1285I CGRYCG 1 cut(s) 84
BshFI GGCC 1 cut(s) 958
BshVI ATCGAT 1 cut(s) 246
BsiEI CGRYCG 1 cut(s) 84
BsiHKAI GWGCWC 1 cut(s) 513
BsiHKCI CYCGRG 2 cut(s) 401, 896
BsiSI CCGG 2 cut(s) 15, 959
BsmAI GTCTC 2 cut(s) 490, 872
BsnI GGCC 1 cut(s) 958
Bso31I GGTCTC 1 cut(s) 490
BsoBI CYCGRG 2 cut(s) 401, 896
Bsp1286I GDGCHC 1 cut(s) 513
Bsp1407I TGTACA 1 cut(s) 685
Bsp143I GATC 6 cut(s) 100, 206, 243, 247, 471, 765
BspACI CCGC 1 cut(s) 130
BspANI GGCC 1 cut(s) 958
BspCNI CTCAG 1 cut(s) 500
BspDI ATCGAT 1 cut(s) 246
BspFNI CGCG 2 cut(s) 94, 422
BspLI GGNNCC 1 cut(s) 752
BspTNI GGTCTC 1 cut(s) 490
BsrBI CCGCTC 1 cut(s) 130
BsrGI TGTACA 1 cut(s) 685
BsrI ACTGG 3 cut(s) 45, 304, 341
BssECI CCNNGG 1 cut(s) 231
BssHII GCGCGC 1 cut(s) 92
BssMI GATC 6 cut(s) 100, 206, 243, 247, 471, 765
BssT1I CCWWGG 1 cut(s) 231
Bst4CI ACNGT 5 cut(s) 58, 524, 673, 784, 974
Bst6I CTCTTC 2 cut(s) 116, 756
BstAPI GCANNNNNTGC 1 cut(s) 143
BstAUI TGTACA 1 cut(s) 685
BstC8I GCNNGC 2 cut(s) 94, 117
BstDEI CTNAG 1 cut(s) 487
BstF5I GGATG 2 cut(s) 138, 474
BstFNI CGCG 2 cut(s) 94, 422
BstHHI GCGC 4 cut(s) 94, 96, 694, 865
BstKTI GATC 6 cut(s) 103, 209, 246, 250, 474, 768
BstMAI GTCTC 2 cut(s) 490, 872
BstMBI GATC 6 cut(s) 100, 206, 243, 247, 471, 765
BstMCI CGRYCG 1 cut(s) 84
BstMWI GCNNNNNNNGC 4 cut(s) 24, 143, 337, 618
BstNSI RCATGY 1 cut(s) 692
BstUI CGCG 2 cut(s) 94, 422
BstV1I GCAGC 5 cut(s) 124, 163, 349, 618, 621
Bsu15I ATCGAT 1 cut(s) 246
BsuRI GGCC 1 cut(s) 958
BsuTUI ATCGAT 1 cut(s) 246
BtsCI GGATG 2 cut(s) 138, 474
BtsI GCAGTG 1 cut(s) 276
BtsIMutI CAGTG 4 cut(s) 276, 348, 789, 979
Cac8I GCNNGC 2 cut(s) 94, 117
CfoI GCGC 4 cut(s) 94, 96, 694, 865
ClaI ATCGAT 1 cut(s) 246
CseI GACGC 2 cut(s) 71, 411
Csp6I GTAC 5 cut(s) 35, 155, 548, 686, 793
CviAII CATG 6 cut(s) 143, 289, 481, 514, 660, 689
CviQI GTAC 5 cut(s) 35, 155, 548, 686, 793
DdeI CTNAG 1 cut(s) 487
DpnI GATC 6 cut(s) 102, 208, 245, 249, 473, 767
DpnII GATC 6 cut(s) 100, 206, 243, 247, 471, 765
DraI TTTAAA 1 cut(s) 535
Eam1104I CTCTTC 2 cut(s) 116, 756
EarI CTCTTC 2 cut(s) 116, 756
Eco130I CCWWGG 1 cut(s) 231
Eco31I GGTCTC 1 cut(s) 490
Eco88I CYCGRG 2 cut(s) 401, 896
EcoT14I CCWWGG 1 cut(s) 231
EcoT22I ATGCAT 1 cut(s) 148
ErhI CCWWGG 1 cut(s) 231
FaeI CATG 6 cut(s) 146, 292, 484, 517, 663, 692
FalI AAGNNNNNCTT 2 cut(s) 659, 691
FatI CATG 6 cut(s) 142, 288, 480, 513, 659, 688
FblI GTMKAC 2 cut(s) 412, 519
Fnu4HI GCNGC 5 cut(s) 113, 177, 338, 607, 610
FokI GGATG 2 cut(s) 145, 481
Fsp4HI GCNGC 5 cut(s) 113, 177, 338, 607, 610
FspBI CTAG 1 cut(s) 618
GlaI GCGC 4 cut(s) 93, 95, 693, 864
GluI GCNGC 5 cut(s) 113, 177, 338, 607, 610
GsaI CCCAGC 1 cut(s) 27
HaeIII GGCC 1 cut(s) 958
HapII CCGG 2 cut(s) 15, 959
HgaI GACGC 2 cut(s) 71, 411
HhaI GCGC 4 cut(s) 94, 96, 694, 865
Hin1II CATG 6 cut(s) 146, 292, 484, 517, 663, 692
Hin6I GCGC 4 cut(s) 92, 94, 692, 863
HinP1I GCGC 4 cut(s) 92, 94, 692, 863
HincII GTYRAC 4 cut(s) 61, 413, 735, 826
HindII GTYRAC 4 cut(s) 61, 413, 735, 826
HinfI GANTC 2 cut(s) 236, 404
HpaII CCGG 2 cut(s) 15, 959
HphI GGTGA 4 cut(s) 55, 423, 511, 887
Hpy166II GTNNAC 8 cut(s) 61, 276, 413, 520, 735, 795, 826, 948
Hpy188I TCNGA 2 cut(s) 636, 855
Hpy8I GTNNAC 8 cut(s) 61, 276, 413, 520, 735, 795, 826, 948
Hpy99I CGWCG 2 cut(s) 52, 528
HpyCH4III ACNGT 5 cut(s) 58, 524, 673, 784, 974
HpyCH4IV ACGT 1 cut(s) 153
HpyCH4V TGCA 7 cut(s) 146, 179, 337, 366, 457, 561, 931
HpyF10VI GCNNNNNNNGC 4 cut(s) 24, 143, 337, 618
HpyF3I CTNAG 1 cut(s) 487
HpySE526I ACGT 1 cut(s) 153
Hsp92II CATG 6 cut(s) 146, 292, 484, 517, 663, 692
HspAI GCGC 4 cut(s) 92, 94, 692, 863
Kzo9I GATC 6 cut(s) 100, 206, 243, 247, 471, 765
Lsp1109I GCAGC 5 cut(s) 124, 163, 349, 618, 621
LweI GCATC 3 cut(s) 155, 375, 486
MaeI CTAG 1 cut(s) 618
MaeII ACGT 1 cut(s) 153
MaeIII GTNAC 2 cut(s) 43, 429
MalI GATC 6 cut(s) 102, 208, 245, 249, 473, 767
MbiI CCGCTC 1 cut(s) 130
MboI GATC 6 cut(s) 100, 206, 243, 247, 471, 765
MboII GAAGA 4 cut(s) 133, 337, 623, 773
MhlI GDGCHC 1 cut(s) 513
MluCI AATT 6 cut(s) 160, 195, 543, 590, 696, 719
MlyI GAGTC 1 cut(s) 413
MnlI CCTC 4 cut(s) 97, 235, 780, 814
Mph1103I ATGCAT 1 cut(s) 148
MseI TTAA 4 cut(s) 5, 159, 534, 654
MspA1I CMGCKG 1 cut(s) 115
MspI CCGG 2 cut(s) 15, 959
MvnI CGCG 2 cut(s) 94, 422
MwoI GCNNNNNNNGC 4 cut(s) 24, 143, 337, 618
NdeII GATC 6 cut(s) 100, 206, 243, 247, 471, 765
NlaIII CATG 6 cut(s) 146, 292, 484, 517, 663, 692
NlaIV GGNNCC 1 cut(s) 752
NmuCI GTSAC 2 cut(s) 43, 429
NsiI ATGCAT 1 cut(s) 148
NspI RCATGY 1 cut(s) 692
PaeR7I CTCGAG 2 cut(s) 401, 896
PauI GCGCGC 1 cut(s) 92
PfeI GAWTC 1 cut(s) 236
PflFI GACNNNGTC 2 cut(s) 65, 830
PkrI GCNGC 5 cut(s) 114, 178, 339, 608, 611
PleI GAGTC 1 cut(s) 412
PpsI GAGTC 1 cut(s) 412
PshBI ATTAAT 1 cut(s) 654
PspFI CCCAGC 1 cut(s) 23
PspN4I GGNNCC 1 cut(s) 752
PspXI VCTCGAGB 2 cut(s) 401, 896
PsyI GACNNNGTC 2 cut(s) 65, 830
PteI GCGCGC 1 cut(s) 92
PvuII CAGCTG 1 cut(s) 115
RsaI GTAC 5 cut(s) 36, 156, 549, 687, 794
RsaNI GTAC 5 cut(s) 35, 155, 548, 686, 793
SalI GTCGAC 1 cut(s) 411
SaqAI TTAA 4 cut(s) 5, 159, 534, 654
SatI GCNGC 5 cut(s) 113, 177, 338, 607, 610
Sau3AI GATC 6 cut(s) 100, 206, 243, 247, 471, 765
ScaI AGTACT 1 cut(s) 36
SchI GAGTC 1 cut(s) 413
SduI GDGCHC 1 cut(s) 513
SetI ASST 9 cut(s) 117, 156, 233, 353, 439, 498, 623, 825, 897
SfaNI GCATC 3 cut(s) 155, 375, 486
Sfr274I CTCGAG 2 cut(s) 401, 896
SlaI CTCGAG 2 cut(s) 401, 896
SmlI CTYRAG 2 cut(s) 401, 896
SmoI CTYRAG 2 cut(s) 401, 896
Sse9I AATT 6 cut(s) 160, 195, 543, 590, 696, 719
SsiI CCGC 1 cut(s) 130
SspI AATATT 1 cut(s) 396
SspMI CTAG 1 cut(s) 618
StyI CCWWGG 1 cut(s) 231
TaaI ACNGT 5 cut(s) 58, 524, 673, 784, 974
TaiI ACGT 1 cut(s) 156
TaqI TCGA 6 cut(s) 246, 402, 412, 577, 897, 915
TaqII GACCGA 1 cut(s) 70
TasI AATT 6 cut(s) 160, 195, 543, 590, 696, 719
TatI WGTACW 2 cut(s) 34, 685
TfiI GAWTC 1 cut(s) 236
Tru1I TTAA 4 cut(s) 5, 159, 534, 654
Tru9I TTAA 4 cut(s) 5, 159, 534, 654
TscAI CASTG 4 cut(s) 283, 348, 789, 979
TseFI GTSAC 2 cut(s) 43, 429
TseI GCWGC 5 cut(s) 112, 176, 337, 606, 609
Tsp45I GTSAC 2 cut(s) 43, 429
TspDTI ATGAA 5 cut(s) 122, 131, 305, 461, 555
TspGWI ACGGA 1 cut(s) 247
TspRI CASTG 4 cut(s) 283, 348, 789, 979
Tth111I GACNNNGTC 2 cut(s) 65, 830
VspI ATTAAT 1 cut(s) 654
XapI RAATTY 1 cut(s) 719
XceI RCATGY 1 cut(s) 692
XhoI CTCGAG 2 cut(s) 401, 896
XmiI GTMKAC 2 cut(s) 412, 519
XspI CTAG 1 cut(s) 618
ZrmI AGTACT 1 cut(s) 36
Zsp2I ATGCAT 1 cut(s) 148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.