Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0005973

Physical Location & Seq

Genomic Coordinates Forward (+)

36730493 .. 36733465

Gene Structure 2973 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1235400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1197 bp

ATGGAGATTAGTTCGGCCACTTCTGGAGGCCGAAGTGACACACTTCTGGTGTCAGAAGACGGCGGCTCGTGGAGGCTCGACGTTAAGGAGTTCCGGCTACCCCAGCAGAGCAGTACTACTGGTGACGACGATGACAGTGGACGCCGTCGTTTCGCTCTCGGTCGCCTCTGCGCGCCCACGAAGAAATACAAAGTTGAAGAATATTATAAGCAACAAGAAAGGCTTCTTGAAGGGTTCAGTGAGATGGAGACCATGACCGCTGAGGGTTGTTCACCAGGTCTTACAGAGGATCAAATGAAGCAGCTAGCGAAGAGCGAGCGGATGGCAGTTCATGTATCAAACGTAGTTAATTTGGTGCTTTTTGCTGCAAAAGTTTATGCTTCAATTATGAGCAGATCATTGTCAGTTATCGCCTCTACCTTGGATTCCCTCTTGGATCTCTTATCCGGCTTTATTTTGTGGTTCACTGCTAATGCCATGAAAAATCCCAACCAGTTTCGCTATCCTATTGGGAAGAAGCGTATGCAGCCAGTGGGTATCATTGTCTTTGCATCTGTTATGGCTACTCTTGGACTGCAAATATTGCTCGAGTCTGGTCGACAACTCGCGTCAAAGTCACCACCGGAAAAAATGAATAGTATGCAGGAGAATTGGATGATCGGCATCATGGTCTCAGTCACAGTGGTGAAGTTTGTGCTCATGGTCTACTGTCGTAGATTTAAAAATGAAATTGTACGGGCATATGCACAAGACCATTTTTTCGATGTCATTACTAATTCAGTTGGTTTAGCAGCAGCAGTCCTAGCTGTTCGGTTCTTCTGGTGGATTGATCCTACTGGTGCTATTATAATAGCAGTATACACGATCAATACATGGGCAAAGACAGTCTTGGAGAATGTATGTGCGCTAATTGGACGAACAGCCCCACCCGAATTTTTAGCGAAGTTGACATACTTGATATGGAACCACCACGAAGAGATCAAGCACATTGACACAGTGAGGGCGTACACATTTGGCTCTCATTACTTTGTGGAGGTTGACATAGTCTTGCCAGAAGACTTGCTTTTGAACAAAGCACATAACATTGGCGAGACACTCCAAGAGAAGTTCGAGCAACTCCCAGAAGTCGAATGGGCTTTTGTGCATATAGATTTTGAGTTCACTCACAGGCCGGAACACAAGGACAGTGTATGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_efflux ZT_dimer

Protein Analysis

399

Amino Acids

45.15

Weight (kDa)

5.97

Isoelectric Point (pI)

48.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cation_efflux	PF01545	111 - 303	5e-32	Cation efflux transmembrane domain
ZT_dimer	PF16916	311 - 384	1.1e-06	Cation efflux protein, cytoplasmic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0005973)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G16310 AT1G16310 AT1G79520 AT1G79520 AT1G79520 AT1G79520
fragaria_vesca	FvH4_3g14550 FvH4_3g14550 FvH4_3g14550 FvH4_3g14550 FvH4_3g14550
malus_domestica	MD05G1235400.v1.1 MD10G1211700.v1.1 MD10G1211900.v1.1
prunus_persica	Prupe.4G131000_v2.0.a1 Prupe.4G131000_v2.0.a1
pyrus_communis	pycom05g21230
rosa_chinensis	RchiOBHm_Chr5g0023931
rosa_laevigata	RLG00000032750
rosa_multiflora	Rmu_sc0004175.1_g000012 Rmu_sc0005837.1_g000003
rosa_roxburghii	Rroxscaffold_1G00055290
rosa_rugosa	Rorug05G0080400
rosa_samantha	Rh5AG170800 Rh5BG167800 Rh5CG183300
rosa_wichuraiana	Rw5G015440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	207, 848
AccBSI	CCGCTC	1 cut(s)	319
AccI	GTMKAC	3 cut(s)	598, 705, 858
AccII	CGCG	2 cut(s)	173, 608
AciI	CCGC	3 cut(s)	63, 258, 319
AclWI	GGATC	3 cut(s)	297, 444, 824
AcoI	YGGCCR	1 cut(s)	15
AcsI	RAATTY	1 cut(s)	932
AcyI	GRCGYC	1 cut(s)	142
AfaI	GTAC	3 cut(s)	115, 735, 1007
AgsI	TTSAA	4 cut(s)	197, 230, 384, 1069
AjnI	CCWGG	1 cut(s)	274
AleI	CACNNNNGTG	1 cut(s)	683
AluBI	AGCT	2 cut(s)	304, 806
AluI	AGCT	2 cut(s)	304, 806
Alw21I	GWGCWC	1 cut(s)	699
Alw26I	GTCTC	3 cut(s)	242, 676, 1085
AlwI	GGATC	3 cut(s)	297, 444, 824
Ama87I	CYCGRG	1 cut(s)	587
AoxI	GGCC	3 cut(s)	15, 28, 1169
ApeKI	GCWGC	5 cut(s)	301, 365, 526, 791, 794
ApoI	RAATTY	1 cut(s)	932
ArsI	GACNNNNNNTTYG	2 cut(s)	743, 775
Asp700I	GAANNNNTTC	1 cut(s)	222
AspLEI	GCGC	3 cut(s)	173, 175, 907
AsuHPI	GGTGA	4 cut(s)	134, 264, 609, 697
AsuNHI	GCTAGC	1 cut(s)	304
AvaI	CYCGRG	1 cut(s)	587
BarI	GAAGNNNNNNTAC	2 cut(s)	935, 967
BauI	CACGAG	1 cut(s)	67
BbsI	GAAGAC	2 cut(s)	63, 1062
Bbv12I	GWGCWC	1 cut(s)	699
BbvCI	CCTCAGC	1 cut(s)	261
BbvI	GCAGC	5 cut(s)	313, 352, 538, 803, 806
BccI	CCATC	2 cut(s)	238, 316
BceAI	ACGGC	2 cut(s)	76, 129
BciT130I	CCWGG	1 cut(s)	276
BcoDI	GTCTC	3 cut(s)	242, 676, 1085
BfaI	CTAG	2 cut(s)	305, 803
BisI	GCNGC	6 cut(s)	64, 302, 366, 527, 792, 795
BlsI	GCNGC	6 cut(s)	65, 303, 367, 528, 793, 796
BmcAI	AGTACT	1 cut(s)	115
Bme1390I	CCNGG	1 cut(s)	276
BmeT110I	CYCGRG	1 cut(s)	587
BmiI	GGNNCC	1 cut(s)	965
BmrFI	CCNGG	1 cut(s)	276
BmsI	GCATC	2 cut(s)	560, 672
BmtI	GCTAGC	1 cut(s)	308
BpiI	GAAGAC	2 cut(s)	63, 1062
BpmI	CTGGAG	1 cut(s)	45
Bpu10I	CCTNAGC	1 cut(s)	261
BsaBI	GATNNNNATC	1 cut(s)	662
BsaHI	GRCGYC	1 cut(s)	142
BsaI	GGTCTC	2 cut(s)	242, 676
BsaJI	CCNNGG	1 cut(s)	420
BsaWI	WCCGGW	1 cut(s)	622
Bse1I	ACTGG	4 cut(s)	124, 493, 530, 841
Bse8I	GATNNNNATC	1 cut(s)	662
BseBI	CCWGG	1 cut(s)	276
BseDI	CCNNGG	1 cut(s)	420
BseGI	GGATG	2 cut(s)	327, 660
BseJI	GATNNNNATC	1 cut(s)	662
BseMII	CTCAG	2 cut(s)	252, 687
BseNI	ACTGG	4 cut(s)	124, 493, 530, 841
BsePI	GCGCGC	1 cut(s)	171
BseXI	GCAGC	5 cut(s)	313, 352, 538, 803, 806
BseYI	CCCAGC	1 cut(s)	102
Bsh1236I	CGCG	2 cut(s)	173, 608
Bsh1285I	CGRYCG	1 cut(s)	163
BshFI	GGCC	3 cut(s)	17, 30, 1171
BsiEI	CGRYCG	1 cut(s)	163
BsiHKAI	GWGCWC	1 cut(s)	699
BsiHKCI	CYCGRG	1 cut(s)	587
BsiSI	CCGG	4 cut(s)	94, 447, 623, 1172
BsmAI	GTCTC	3 cut(s)	242, 676, 1085
BsnI	GGCC	3 cut(s)	17, 30, 1171
Bso31I	GGTCTC	2 cut(s)	242, 676
BsoBI	CYCGRG	1 cut(s)	587
Bsp1286I	GDGCHC	1 cut(s)	699
Bsp143I	GATC	7 cut(s)	289, 395, 436, 657, 829, 864, 978
BspACI	CCGC	3 cut(s)	63, 258, 319
BspANI	GGCC	3 cut(s)	17, 30, 1171
BspCNI	CTCAG	2 cut(s)	253, 686
BspFNI	CGCG	2 cut(s)	173, 608
BspLI	GGNNCC	1 cut(s)	965
BspOI	GCTAGC	1 cut(s)	308
BspPI	GGATC	3 cut(s)	297, 444, 824
BspQI	GCTCTTC	1 cut(s)	305
BspTNI	GGTCTC	2 cut(s)	242, 676
BsrBI	CCGCTC	1 cut(s)	319
BsrI	ACTGG	4 cut(s)	124, 493, 530, 841
BssECI	CCNNGG	1 cut(s)	420
BssHII	GCGCGC	1 cut(s)	171
BssMI	GATC	7 cut(s)	289, 395, 436, 657, 829, 864, 978
BssNAI	GTATAC	1 cut(s)	859
BssNI	GRCGYC	1 cut(s)	142
BssSI	CACGAG	1 cut(s)	67
BssT1I	CCWWGG	1 cut(s)	420
Bst1107I	GTATAC	1 cut(s)	859
Bst2BI	CACGAG	1 cut(s)	67
Bst2UI	CCWGG	1 cut(s)	276
Bst4CI	ACNGT	6 cut(s)	137, 682, 710, 886, 997, 1187
Bst6I	CTCTTC	2 cut(s)	305, 969
BstACI	GRCGYC	1 cut(s)	142
BstAPI	GCANNNNNTGC	1 cut(s)	583
BstC8I	GCNNGC	3 cut(s)	173, 306, 317
BstDEI	CTNAG	2 cut(s)	261, 673
BstF5I	GGATG	2 cut(s)	327, 660
BstFNI	CGCG	2 cut(s)	173, 608
BstHHI	GCGC	3 cut(s)	173, 175, 907
BstKTI	GATC	7 cut(s)	292, 398, 439, 660, 832, 867, 981
BstMAI	GTCTC	3 cut(s)	242, 676, 1085
BstMBI	GATC	7 cut(s)	289, 395, 436, 657, 829, 864, 978
BstMCI	CGRYCG	1 cut(s)	163
BstMWI	GCNNNNNNNGC	4 cut(s)	103, 526, 583, 803
BstNI	CCWGG	1 cut(s)	276
BstSCI	CCNGG	1 cut(s)	274
BstUI	CGCG	2 cut(s)	173, 608
BstV1I	GCAGC	5 cut(s)	313, 352, 538, 803, 806
BstV2I	GAAGAC	2 cut(s)	63, 1062
BstX2I	RGATCY	1 cut(s)	436
BstYI	RGATCY	1 cut(s)	436
BstZ17I	GTATAC	1 cut(s)	859
BsuRI	GGCC	3 cut(s)	17, 30, 1171
BtsCI	GGATG	2 cut(s)	327, 660
BtsI	GCAGTG	1 cut(s)	465
BtsIMutI	CAGTG	7 cut(s)	142, 244, 465, 537, 687, 1002, 1192
Cac8I	GCNNGC	3 cut(s)	173, 306, 317
CfoI	GCGC	3 cut(s)	173, 175, 907
CseI	GACGC	2 cut(s)	150, 597
CsiI	ACCWGGT	1 cut(s)	274
Csp6I	GTAC	3 cut(s)	114, 734, 1006
CviAII	CATG	6 cut(s)	253, 332, 478, 667, 700, 873
CviQI	GTAC	3 cut(s)	114, 734, 1006
DdeI	CTNAG	2 cut(s)	261, 673
DpnI	GATC	7 cut(s)	291, 397, 438, 659, 831, 866, 980
DpnII	GATC	7 cut(s)	289, 395, 436, 657, 829, 864, 978
DraI	TTTAAA	1 cut(s)	721
EaeI	YGGCCR	1 cut(s)	15
Eam1104I	CTCTTC	2 cut(s)	305, 969
EarI	CTCTTC	2 cut(s)	305, 969
Eco130I	CCWWGG	1 cut(s)	420
Eco31I	GGTCTC	2 cut(s)	242, 676
Eco88I	CYCGRG	1 cut(s)	587
EcoRII	CCWGG	1 cut(s)	274
EcoT14I	CCWWGG	1 cut(s)	420
ErhI	CCWWGG	1 cut(s)	420
FaeI	CATG	6 cut(s)	256, 335, 481, 670, 703, 876
FalI	AAGNNNNNCTT	6 cut(s)	207, 239, 872, 904, 1047, 1079
FatI	CATG	6 cut(s)	252, 331, 477, 666, 699, 872
FauNDI	CATATG	1 cut(s)	742
FblI	GTMKAC	3 cut(s)	598, 705, 858
Fnu4HI	GCNGC	6 cut(s)	64, 302, 366, 527, 792, 795
FokI	GGATG	2 cut(s)	334, 667
Fsp4HI	GCNGC	6 cut(s)	64, 302, 366, 527, 792, 795
FspBI	CTAG	2 cut(s)	305, 803
GlaI	GCGC	3 cut(s)	172, 174, 906
GluI	GCNGC	6 cut(s)	64, 302, 366, 527, 792, 795
GsaI	CCCAGC	1 cut(s)	106
GsuI	CTGGAG	1 cut(s)	45
HaeIII	GGCC	3 cut(s)	17, 30, 1171
HapII	CCGG	4 cut(s)	94, 447, 623, 1172
HgaI	GACGC	2 cut(s)	150, 597
HhaI	GCGC	3 cut(s)	173, 175, 907
Hin1I	GRCGYC	1 cut(s)	142
Hin1II	CATG	6 cut(s)	256, 335, 481, 670, 703, 876
Hin6I	GCGC	3 cut(s)	171, 173, 905
HinP1I	GCGC	3 cut(s)	171, 173, 905
HincII	GTYRAC	3 cut(s)	599, 948, 1039
HindII	GTYRAC	3 cut(s)	599, 948, 1039
HinfI	GANTC	2 cut(s)	425, 590
HpaII	CCGG	4 cut(s)	94, 447, 623, 1172
HphI	GGTGA	4 cut(s)	134, 264, 609, 697
Hpy188I	TCNGA	1 cut(s)	55
Hpy188III	TCNNGA	2 cut(s)	24, 227
Hpy99I	CGWCG	3 cut(s)	83, 131, 150
HpyAV	CCTTC	1 cut(s)	224
HpyCH4III	ACNGT	6 cut(s)	137, 682, 710, 886, 997, 1187
HpyCH4IV	ACGT	2 cut(s)	81, 342
HpyCH4V	TGCA	7 cut(s)	368, 526, 551, 577, 643, 746, 1144
HpyF10VI	GCNNNNNNNGC	4 cut(s)	103, 526, 583, 803
HpyF3I	CTNAG	2 cut(s)	261, 673
HpySE526I	ACGT	2 cut(s)	81, 342
Hsp92I	GRCGYC	1 cut(s)	142
Hsp92II	CATG	6 cut(s)	256, 335, 481, 670, 703, 876
HspAI	GCGC	3 cut(s)	171, 173, 905
Kzo9I	GATC	7 cut(s)	289, 395, 436, 657, 829, 864, 978
LguI	GCTCTTC	1 cut(s)	305
Lsp1109I	GCAGC	5 cut(s)	313, 352, 538, 803, 806
LweI	GCATC	2 cut(s)	560, 672
MabI	ACCWGGT	1 cut(s)	274
MaeI	CTAG	2 cut(s)	305, 803
MaeII	ACGT	2 cut(s)	81, 342
MaeIII	GTNAC	4 cut(s)	35, 122, 615, 676
MalI	GATC	7 cut(s)	291, 397, 438, 659, 831, 866, 980
MbiI	CCGCTC	1 cut(s)	319
MboI	GATC	7 cut(s)	289, 395, 436, 657, 829, 864, 978
MboII	GAAGA	8 cut(s)	68, 193, 209, 322, 526, 808, 986, 1067
MflI	RGATCY	1 cut(s)	436
MhlI	GDGCHC	1 cut(s)	699
MluCI	AATT	7 cut(s)	349, 384, 649, 729, 775, 909, 932
MlyI	GAGTC	1 cut(s)	599
MnlI	CCTC	9 cut(s)	20, 66, 176, 256, 280, 424, 440, 993, 1027
MroXI	GAANNNNTTC	1 cut(s)	222
MseI	TTAA	3 cut(s)	84, 348, 720
MslI	CAYNNNNRTG	1 cut(s)	683
MspA1I	CMGCKG	1 cut(s)	260
MspI	CCGG	4 cut(s)	94, 447, 623, 1172
MspR9I	CCNGG	1 cut(s)	276
MvaI	CCWGG	1 cut(s)	276
MvnI	CGCG	2 cut(s)	173, 608
MwoI	GCNNNNNNNGC	4 cut(s)	103, 526, 583, 803
NdeI	CATATG	1 cut(s)	742
NdeII	GATC	7 cut(s)	289, 395, 436, 657, 829, 864, 978
NheI	GCTAGC	1 cut(s)	304
NlaIII	CATG	6 cut(s)	256, 335, 481, 670, 703, 876
NlaIV	GGNNCC	1 cut(s)	965
NmuCI	GTSAC	4 cut(s)	35, 122, 615, 676
OliI	CACNNNNGTG	1 cut(s)	683
PaeR7I	CTCGAG	1 cut(s)	587
PauI	GCGCGC	1 cut(s)	171
PciSI	GCTCTTC	1 cut(s)	305
PdmI	GAANNNNTTC	1 cut(s)	222
PfeI	GAWTC	1 cut(s)	425
PflFI	GACNNNGTC	2 cut(s)	144, 1043
PkrI	GCNGC	6 cut(s)	65, 303, 367, 528, 793, 796
PleI	GAGTC	1 cut(s)	598
PpsI	GAGTC	1 cut(s)	598
PsiI	TTATAA	2 cut(s)	207, 848
Psp6I	CCWGG	1 cut(s)	274
PspFI	CCCAGC	1 cut(s)	102
PspGI	CCWGG	1 cut(s)	274
PspN4I	GGNNCC	1 cut(s)	965
PspXI	VCTCGAGB	1 cut(s)	587
PsuI	RGATCY	1 cut(s)	436
PsyI	GACNNNGTC	2 cut(s)	144, 1043
PteI	GCGCGC	1 cut(s)	171
RsaI	GTAC	3 cut(s)	115, 735, 1007
RsaNI	GTAC	3 cut(s)	114, 734, 1006
RseI	CAYNNNNRTG	1 cut(s)	683
SalI	GTCGAC	1 cut(s)	597
SapI	GCTCTTC	1 cut(s)	305
SaqAI	TTAA	3 cut(s)	84, 348, 720
SatI	GCNGC	6 cut(s)	64, 302, 366, 527, 792, 795
Sau3AI	GATC	7 cut(s)	289, 395, 436, 657, 829, 864, 978
ScaI	AGTACT	1 cut(s)	115
SchI	GAGTC	1 cut(s)	599
ScrFI	CCNGG	1 cut(s)	276
SduI	GDGCHC	1 cut(s)	699
SetI	ASST	7 cut(s)	84, 280, 306, 345, 422, 808, 1038
SexAI	ACCWGGT	1 cut(s)	274
SfaNI	GCATC	2 cut(s)	560, 672
Sfr274I	CTCGAG	1 cut(s)	587
SlaI	CTCGAG	1 cut(s)	587
SmiMI	CAYNNNNRTG	1 cut(s)	683
SmlI	CTYRAG	1 cut(s)	587
SmoI	CTYRAG	1 cut(s)	587
Sse9I	AATT	7 cut(s)	349, 384, 649, 729, 775, 909, 932
SsiI	CCGC	3 cut(s)	63, 258, 319
SspI	AATATT	2 cut(s)	203, 582
SspMI	CTAG	2 cut(s)	305, 803
StyD4I	CCNGG	1 cut(s)	274
StyI	CCWWGG	1 cut(s)	420
TaaI	ACNGT	6 cut(s)	137, 682, 710, 886, 997, 1187
TaiI	ACGT	2 cut(s)	84, 345
TaqI	TCGA	6 cut(s)	78, 588, 598, 762, 1110, 1128
TaqII	GACCGA	1 cut(s)	149
TasI	AATT	7 cut(s)	349, 384, 649, 729, 775, 909, 932
TatI	WGTACW	1 cut(s)	113
TauI	GCSGC	1 cut(s)	66
TfiI	GAWTC	1 cut(s)	425
Tru1I	TTAA	3 cut(s)	84, 348, 720
Tru9I	TTAA	3 cut(s)	84, 348, 720
TscAI	CASTG	7 cut(s)	142, 244, 472, 537, 687, 1002, 1192
TseFI	GTSAC	4 cut(s)	35, 122, 615, 676
TseI	GCWGC	5 cut(s)	301, 365, 526, 791, 794
Tsp45I	GTSAC	4 cut(s)	35, 122, 615, 676
TspDTI	ATGAA	5 cut(s)	311, 320, 494, 647, 741
TspRI	CASTG	7 cut(s)	142, 244, 472, 537, 687, 1002, 1192
Tth111I	GACNNNGTC	2 cut(s)	144, 1043
XapI	RAATTY	1 cut(s)	932
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1128
XhoI	CTCGAG	1 cut(s)	587
XmiI	GTMKAC	3 cut(s)	598, 705, 858
XmnI	GAANNNNTTC	1 cut(s)	222
XspI	CTAG	2 cut(s)	305, 803
ZrmI	AGTACT	1 cut(s)	115

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.