Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0005973

Physical Location & Seq

Genomic Coordinates Forward (+)

31077184 .. 31080068

Gene Structure 2885 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1211700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1266 bp

ATGGAGATTAGTTCGCCCACTTCCGGAGGCCGCACTGAACCACTTCTGGTGTCACAAGACGACGCCGTACAGCCGGCCGGAAATACATCGTGGAGACTCGATGTTAAGGAGTTCCGGCTGCCCCAGCAGAACGGTACTAGTGGTGACGACCATGACAGTGGAAGGAGTCGTTTCGCATTCCGTCGCCTCTGCGCCCCCAGTAAGGATTCTGCTCATGAATTTTTCTTGCTTTGGCTTTTAAACTCAGGGAAAAAGTACAAGGTTGAAGAATATTACAAGAAACAGGAAAGGCTTCTTGAAGGGTTCAGTGAGATGGAGACCATGACTGAAGAGGGTTGGTTGCCCGGAAGTCTTACGGAGGATCAAATGAAGCAGCTAGCGAAGAGCGAGCGGATGGCAGTTCATGCATCAAACGTAGCTAATTTGGTTCTTTTTGCTGCAAAAGTTTATGCTTCAATTATGAGCAGATCATTGGCAGTTATTGCTTCTACCTTGGATTCCCTCTTGGATCTCTTATCCGGCTTTATTTTGTGGTTCACTGCTAATGCCATGAAAAATCCCAACCAGTTTCGCTATCCTATTGGGAAGAAACGTATGCAACCAGTGGGTATCATTGTCTTCGCATCTGTTATGGCTACTCTTGGACTGCAAATATTGATCGAGTCTGGTCGAGAACTCGTCTCTCAGTCACCACCCGAAAAAATGAATAGTACGCAGGAGAATTGGATGATCGGCATCATGGTCTCAGTCACTGTAGTGAAGTTTGTGCTCATGGTCTACTGTCGTAGATTTAAGAATGCAATTGTACGGGCCTATGCACAAGACCATTTTTTCGATGTCATTACTAATTCAGTTGGTTTAGCAGCAGCAGTCCTAGCTGTCCGGTTCTTCTGGTGGATTGATCCTACTGGTGCTATTATAATAGCACTATATACCATTAACACATGGGCAAAGACAGTCTTGGAGAATGTACATGCGCTAATTGGAAGAACAGCACCACCCGACTTTTTGGCAAAGTTGACATACTTGATATGGAACCACCATGAAGAGATCAAGCACATCGACACAGTGAGGGCGTACACATTTGGTTCCCATTACTTTGTGGAGGTTGACATTGTCTTGCCAGAAGACATGCTACTGAACAAAGCGCACAACATTGGCGAGACTCTCCAAGAGAAGCTCGAGCAACTCCCTGAAGTGGAACGGGCTTTTGTGCATATAGATTTTGAGTTCACTCACAGGCCGGAACACAAGAACAGTGTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_efflux ZT_dimer

Protein Analysis

422

Amino Acids

47.78

Weight (kDa)

6.25

Isoelectric Point (pI)

49.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cation_efflux	PF01545	135 - 327	2.9e-32	Cation efflux transmembrane domain
ZT_dimer	PF16916	342 - 408	1e-08	Cation efflux protein, cytoplasmic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0005973)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G16310 AT1G16310 AT1G79520 AT1G79520 AT1G79520 AT1G79520
fragaria_vesca	FvH4_3g14550 FvH4_3g14550 FvH4_3g14550 FvH4_3g14550 FvH4_3g14550
malus_domestica	MD05G1235400.v1.1 MD10G1211700.v1.1 MD10G1211900.v1.1
prunus_persica	Prupe.4G131000_v2.0.a1 Prupe.4G131000_v2.0.a1
pyrus_communis	pycom05g21230
rosa_chinensis	RchiOBHm_Chr5g0023931
rosa_laevigata	RLG00000032750
rosa_multiflora	Rmu_sc0004175.1_g000012 Rmu_sc0005837.1_g000003
rosa_roxburghii	Rroxscaffold_1G00055290
rosa_rugosa	Rorug05G0080400
rosa_samantha	Rh5AG170800 Rh5BG167800 Rh5CG183300
rosa_wichuraiana	Rw5G015440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	920
AccBSI	CCGCTC	1 cut(s)	391
AccI	GTMKAC	1 cut(s)	777
AccIII	TCCGGA	1 cut(s)	23
AciI	CCGC	2 cut(s)	31, 391
AclWI	GGATC	3 cut(s)	369, 516, 896
AcoI	YGGCCR	1 cut(s)	75
AcsI	RAATTY	1 cut(s)	218
AcuI	CTGAAG	2 cut(s)	348, 1215
AcyI	GRCGYC	1 cut(s)	63
AfaI	GTAC	7 cut(s)	69, 136, 257, 712, 807, 972, 1079
AfiI	CCNNNNNNNGG	3 cut(s)	23, 202, 1198
AgsI	TTSAA	3 cut(s)	266, 299, 456
AhlI	ACTAGT	1 cut(s)	137
AleI	CACNNNNGTG	1 cut(s)	755
AluBI	AGCT	4 cut(s)	376, 419, 878, 1180
AluI	AGCT	4 cut(s)	376, 419, 878, 1180
Alw21I	GWGCWC	1 cut(s)	771
Alw26I	GTCTC	5 cut(s)	88, 311, 685, 748, 1157
AlwI	GGATC	3 cut(s)	369, 516, 896
AlwNI	CAGNNNCTG	1 cut(s)	752
Ama87I	CYCGRG	1 cut(s)	1181
Aor13HI	TCCGGA	1 cut(s)	23
AoxI	GGCC	4 cut(s)	28, 75, 810, 1241
ApeKI	GCWGC	5 cut(s)	118, 373, 437, 863, 866
ApoI	RAATTY	1 cut(s)	218
ArsI	GACNNNNNNTTYG	2 cut(s)	815, 847
Asp700I	GAANNNNTTC	2 cut(s)	42, 291
AspLEI	GCGC	3 cut(s)	194, 979, 1150
AspS9I	GGNCC	1 cut(s)	810
AsuC2I	CCSGG	1 cut(s)	345
AsuHPI	GGTGA	2 cut(s)	155, 681
AsuNHI	GCTAGC	1 cut(s)	376
AvaI	CYCGRG	1 cut(s)	1181
BarI	GAAGNNNNNNTAC	2 cut(s)	1119, 1151
BbsI	GAAGAC	2 cut(s)	610, 1134
Bbv12I	GWGCWC	1 cut(s)	771
BbvI	GCAGC	5 cut(s)	105, 385, 424, 875, 878
BccI	CCATC	2 cut(s)	307, 388
BceAI	ACGGC	1 cut(s)	50
BcnI	CCSGG	1 cut(s)	345
BcoDI	GTCTC	5 cut(s)	88, 311, 685, 748, 1157
BcuI	ACTAGT	1 cut(s)	137
BfaI	CTAG	3 cut(s)	138, 377, 875
BfmI	CTRYAG	1 cut(s)	753
BisI	GCNGC	6 cut(s)	31, 119, 374, 438, 864, 867
BlsI	GCNGC	6 cut(s)	32, 120, 375, 439, 865, 868
Bme1390I	CCNGG	1 cut(s)	345
BmeT110I	CYCGRG	1 cut(s)	1181
BmgT120I	GGNCC	1 cut(s)	810
BmiI	GGNNCC	2 cut(s)	1037, 1090
BmrFI	CCNGG	1 cut(s)	345
BmrI	ACTGGG	1 cut(s)	192
BmsI	GCATC	3 cut(s)	416, 632, 744
BmtI	GCTAGC	1 cut(s)	380
BmuI	ACTGGG	1 cut(s)	192
BpiI	GAAGAC	2 cut(s)	610, 1134
BpuMI	CCSGG	1 cut(s)	345
BsaBI	GATNNNNATC	1 cut(s)	734
BsaHI	GRCGYC	1 cut(s)	63
BsaI	GGTCTC	2 cut(s)	311, 748
BsaJI	CCNNGG	1 cut(s)	492
BsaWI	WCCGGW	2 cut(s)	23, 882
Bsc4I	CCNNNNNNNGG	3 cut(s)	23, 202, 1198
Bse118I	RCCGGY	1 cut(s)	73
Bse1I	ACTGG	4 cut(s)	198, 565, 602, 913
Bse8I	GATNNNNATC	1 cut(s)	734
BseAI	TCCGGA	1 cut(s)	23
BseDI	CCNNGG	1 cut(s)	492
BseGI	GGATG	2 cut(s)	399, 732
BseJI	GATNNNNATC	1 cut(s)	734
BseLI	CCNNNNNNNGG	3 cut(s)	23, 202, 1198
BseMII	CTCAG	3 cut(s)	258, 698, 759
BseNI	ACTGG	4 cut(s)	198, 565, 602, 913
BseX3I	CGGCCG	1 cut(s)	75
BseXI	GCAGC	5 cut(s)	105, 385, 424, 875, 878
BseYI	CCCAGC	1 cut(s)	123
Bsh1285I	CGRYCG	1 cut(s)	78
BshFI	GGCC	4 cut(s)	30, 77, 812, 1243
BsiEI	CGRYCG	1 cut(s)	78
BsiHKAI	GWGCWC	1 cut(s)	771
BsiHKCI	CYCGRG	1 cut(s)	1181
BsiSI	CCGG	8 cut(s)	24, 74, 78, 115, 345, 519, 883, 1244
BslI	CCNNNNNNNGG	3 cut(s)	23, 202, 1198
BsmAI	GTCTC	5 cut(s)	88, 311, 685, 748, 1157
BsmBI	CGTCTC	1 cut(s)	685
BsmI	GAATGC	2 cut(s)	176, 802
BsnI	GGCC	4 cut(s)	30, 77, 812, 1243
Bso31I	GGTCTC	2 cut(s)	311, 748
BsoBI	CYCGRG	1 cut(s)	1181
Bsp1286I	GDGCHC	1 cut(s)	771
Bsp13I	TCCGGA	1 cut(s)	23
Bsp1407I	TGTACA	1 cut(s)	970
Bsp143I	GATC	7 cut(s)	361, 467, 508, 657, 729, 901, 1050
BspACI	CCGC	2 cut(s)	31, 391
BspANI	GGCC	4 cut(s)	30, 77, 812, 1243
BspCNI	CTCAG	3 cut(s)	257, 697, 758
BspEI	TCCGGA	1 cut(s)	23
BspHI	TCATGA	1 cut(s)	214
BspLI	GGNNCC	2 cut(s)	1037, 1090
BspOI	GCTAGC	1 cut(s)	380
BspPI	GGATC	3 cut(s)	369, 516, 896
BspQI	GCTCTTC	1 cut(s)	377
BspTNI	GGTCTC	2 cut(s)	311, 748
BsrBI	CCGCTC	1 cut(s)	391
BsrFI	RCCGGY	1 cut(s)	73
BsrGI	TGTACA	1 cut(s)	970
BsrI	ACTGG	4 cut(s)	198, 565, 602, 913
BssAI	RCCGGY	1 cut(s)	73
BssECI	CCNNGG	1 cut(s)	492
BssMI	GATC	7 cut(s)	361, 467, 508, 657, 729, 901, 1050
BssNI	GRCGYC	1 cut(s)	63
BssT1I	CCWWGG	1 cut(s)	492
Bst4CI	ACNGT	7 cut(s)	134, 158, 754, 782, 958, 1069, 1259
Bst6I	CTCTTC	3 cut(s)	324, 377, 1041
BstACI	GRCGYC	1 cut(s)	63
BstAPI	GCANNNNNTGC	2 cut(s)	404, 482
BstAUI	TGTACA	1 cut(s)	970
BstC8I	GCNNGC	3 cut(s)	75, 378, 389
BstDEI	CTNAG	3 cut(s)	244, 684, 745
BstF5I	GGATG	2 cut(s)	399, 732
BstHHI	GCGC	3 cut(s)	194, 979, 1150
BstKTI	GATC	7 cut(s)	364, 470, 511, 660, 732, 904, 1053
BstMAI	GTCTC	5 cut(s)	88, 311, 685, 748, 1157
BstMBI	GATC	7 cut(s)	361, 467, 508, 657, 729, 901, 1050
BstMCI	CGRYCG	1 cut(s)	78
BstMWI	GCNNNNNNNGC	4 cut(s)	124, 404, 482, 875
BstNSI	RCATGY	2 cut(s)	977, 1135
BstSCI	CCNGG	1 cut(s)	343
BstSFI	CTRYAG	1 cut(s)	753
BstV1I	GCAGC	5 cut(s)	105, 385, 424, 875, 878
BstV2I	GAAGAC	2 cut(s)	610, 1134
BstX2I	RGATCY	1 cut(s)	508
BstXI	CCANNNNNNTGG	1 cut(s)	158
BstYI	RGATCY	1 cut(s)	508
BstZI	CGGCCG	1 cut(s)	75
BsuRI	GGCC	4 cut(s)	30, 77, 812, 1243
BtsCI	GGATG	2 cut(s)	399, 732
BtsI	GCAGTG	1 cut(s)	537
BtsIMutI	CAGTG	8 cut(s)	33, 163, 313, 537, 609, 750, 1074, 1264
Cac8I	GCNNGC	3 cut(s)	75, 378, 389
CaiI	CAGNNNCTG	1 cut(s)	752
CciI	TCATGA	1 cut(s)	214
CfoI	GCGC	3 cut(s)	194, 979, 1150
Cfr10I	RCCGGY	1 cut(s)	73
Cfr13I	GGNCC	1 cut(s)	810
CseI	GACGC	1 cut(s)	71
Csp6I	GTAC	7 cut(s)	68, 135, 256, 711, 806, 971, 1078
CviQI	GTAC	7 cut(s)	68, 135, 256, 711, 806, 971, 1078
DdeI	CTNAG	3 cut(s)	244, 684, 745
DpnI	GATC	7 cut(s)	363, 469, 510, 659, 731, 903, 1052
DpnII	GATC	7 cut(s)	361, 467, 508, 657, 729, 901, 1050
DraI	TTTAAA	1 cut(s)	240
EaeI	YGGCCR	1 cut(s)	75
EagI	CGGCCG	1 cut(s)	75
Eam1104I	CTCTTC	3 cut(s)	324, 377, 1041
EarI	CTCTTC	3 cut(s)	324, 377, 1041
EclXI	CGGCCG	1 cut(s)	75
Eco130I	CCWWGG	1 cut(s)	492
Eco31I	GGTCTC	2 cut(s)	311, 748
Eco52I	CGGCCG	1 cut(s)	75
Eco57I	CTGAAG	2 cut(s)	348, 1215
Eco88I	CYCGRG	1 cut(s)	1181
EcoT14I	CCWWGG	1 cut(s)	492
EcoT22I	ATGCAT	1 cut(s)	409
ErhI	CCWWGG	1 cut(s)	492
Esp3I	CGTCTC	1 cut(s)	685
FalI	AAGNNNNNCTT	2 cut(s)	944, 976
FblI	GTMKAC	1 cut(s)	777
Fnu4HI	GCNGC	6 cut(s)	31, 119, 374, 438, 864, 867
FokI	GGATG	2 cut(s)	406, 739
Fsp4HI	GCNGC	6 cut(s)	31, 119, 374, 438, 864, 867
FspBI	CTAG	3 cut(s)	138, 377, 875
GlaI	GCGC	3 cut(s)	193, 978, 1149
GluI	GCNGC	6 cut(s)	31, 119, 374, 438, 864, 867
GsaI	CCCAGC	1 cut(s)	127
HaeIII	GGCC	4 cut(s)	30, 77, 812, 1243
HapII	CCGG	8 cut(s)	24, 74, 78, 115, 345, 519, 883, 1244
HgaI	GACGC	1 cut(s)	71
HhaI	GCGC	3 cut(s)	194, 979, 1150
Hin1I	GRCGYC	1 cut(s)	63
Hin6I	GCGC	3 cut(s)	192, 977, 1148
HinP1I	GCGC	3 cut(s)	192, 977, 1148
HincII	GTYRAC	2 cut(s)	1020, 1111
HindII	GTYRAC	2 cut(s)	1020, 1111
HinfI	GANTC	6 cut(s)	96, 166, 206, 497, 662, 1165
HpaII	CCGG	8 cut(s)	24, 74, 78, 115, 345, 519, 883, 1244
HphI	GGTGA	2 cut(s)	155, 681
Hpy166II	GTNNAC	6 cut(s)	537, 778, 1020, 1080, 1111, 1233
Hpy188III	TCNNGA	4 cut(s)	24, 215, 296, 671
Hpy8I	GTNNAC	6 cut(s)	537, 778, 1020, 1080, 1111, 1233
Hpy99I	CGWCG	2 cut(s)	65, 186
HpyAV	CCTTC	2 cut(s)	156, 293
HpyCH4III	ACNGT	7 cut(s)	134, 158, 754, 782, 958, 1069, 1259
HpyCH4IV	ACGT	2 cut(s)	414, 592
HpyCH4V	TGCA	7 cut(s)	407, 440, 598, 649, 800, 818, 1216
HpyF10VI	GCNNNNNNNGC	4 cut(s)	124, 404, 482, 875
HpyF3I	CTNAG	3 cut(s)	244, 684, 745
HpySE526I	ACGT	2 cut(s)	414, 592
Hsp92I	GRCGYC	1 cut(s)	63
HspAI	GCGC	3 cut(s)	192, 977, 1148
Kpn2I	TCCGGA	1 cut(s)	23
KroI	GCCGGC	1 cut(s)	73
KroNI	GCCGGC	1 cut(s)	75
Kzo9I	GATC	7 cut(s)	361, 467, 508, 657, 729, 901, 1050
LguI	GCTCTTC	1 cut(s)	377
Lsp1109I	GCAGC	5 cut(s)	105, 385, 424, 875, 878
LweI	GCATC	3 cut(s)	416, 632, 744
MaeI	CTAG	3 cut(s)	138, 377, 875
MaeII	ACGT	2 cut(s)	414, 592
MaeIII	GTNAC	4 cut(s)	51, 143, 687, 748
MalI	GATC	7 cut(s)	363, 469, 510, 659, 731, 903, 1052
MbiI	CCGCTC	1 cut(s)	391
MboI	GATC	7 cut(s)	361, 467, 508, 657, 729, 901, 1050
MboII	GAAGA	9 cut(s)	278, 341, 394, 598, 610, 880, 999, 1058, 1139
MfeI	CAATTG	1 cut(s)	801
MflI	RGATCY	1 cut(s)	508
MhlI	GDGCHC	1 cut(s)	771
MluCI	AATT	7 cut(s)	218, 421, 456, 721, 801, 847, 981
MlyI	GAGTC	4 cut(s)	90, 175, 671, 1159
MnlI	CCTC	7 cut(s)	20, 197, 325, 352, 512, 1065, 1099
Mph1103I	ATGCAT	1 cut(s)	409
MroI	TCCGGA	1 cut(s)	23
MroNI	GCCGGC	1 cut(s)	73
MroXI	GAANNNNTTC	2 cut(s)	42, 291
MseI	TTAA	4 cut(s)	105, 239, 792, 939
MslI	CAYNNNNRTG	2 cut(s)	156, 755
MspI	CCGG	8 cut(s)	24, 74, 78, 115, 345, 519, 883, 1244
MspR9I	CCNGG	1 cut(s)	345
MunI	CAATTG	1 cut(s)	801
Mva1269I	GAATGC	2 cut(s)	176, 802
MwoI	GCNNNNNNNGC	4 cut(s)	124, 404, 482, 875
NaeI	GCCGGC	1 cut(s)	75
NciI	CCSGG	1 cut(s)	345
NdeII	GATC	7 cut(s)	361, 467, 508, 657, 729, 901, 1050
NgoMIV	GCCGGC	1 cut(s)	73
NheI	GCTAGC	1 cut(s)	376
NlaIV	GGNNCC	2 cut(s)	1037, 1090
NmuCI	GTSAC	4 cut(s)	51, 143, 687, 748
NsiI	ATGCAT	1 cut(s)	409
NspI	RCATGY	2 cut(s)	977, 1135
OliI	CACNNNNGTG	1 cut(s)	755
PaeR7I	CTCGAG	1 cut(s)	1181
PagI	TCATGA	1 cut(s)	214
PciSI	GCTCTTC	1 cut(s)	377
PctI	GAATGC	2 cut(s)	176, 802
PdiI	GCCGGC	1 cut(s)	75
PdmI	GAANNNNTTC	2 cut(s)	42, 291
PfeI	GAWTC	2 cut(s)	206, 497
PflFI	GACNNNGTC	1 cut(s)	1115
PkrI	GCNGC	6 cut(s)	32, 120, 375, 439, 865, 868
PleI	GAGTC	4 cut(s)	90, 174, 670, 1159
PpsI	GAGTC	4 cut(s)	90, 174, 670, 1159
PsiI	TTATAA	1 cut(s)	920
PspFI	CCCAGC	1 cut(s)	123
PspN4I	GGNNCC	2 cut(s)	1037, 1090
PspPI	GGNCC	1 cut(s)	810
PspXI	VCTCGAGB	1 cut(s)	1181
PstNI	CAGNNNCTG	1 cut(s)	752
PsuI	RGATCY	1 cut(s)	508
PsyI	GACNNNGTC	1 cut(s)	1115
RsaI	GTAC	7 cut(s)	69, 136, 257, 712, 807, 972, 1079
RsaNI	GTAC	7 cut(s)	68, 135, 256, 711, 806, 971, 1078
RseI	CAYNNNNRTG	2 cut(s)	156, 755
SapI	GCTCTTC	1 cut(s)	377
SaqAI	TTAA	4 cut(s)	105, 239, 792, 939
SatI	GCNGC	6 cut(s)	31, 119, 374, 438, 864, 867
Sau3AI	GATC	7 cut(s)	361, 467, 508, 657, 729, 901, 1050
Sau96I	GGNCC	1 cut(s)	810
SchI	GAGTC	4 cut(s)	90, 175, 671, 1159
ScrFI	CCNGG	1 cut(s)	345
SduI	GDGCHC	1 cut(s)	771
SetI	ASST	9 cut(s)	264, 378, 417, 421, 494, 595, 880, 1110, 1182
SfaNI	GCATC	3 cut(s)	416, 632, 744
SfcI	CTRYAG	1 cut(s)	753
Sfr274I	CTCGAG	1 cut(s)	1181
SlaI	CTCGAG	1 cut(s)	1181
SmiMI	CAYNNNNRTG	2 cut(s)	156, 755
SmlI	CTYRAG	1 cut(s)	1181
SmoI	CTYRAG	1 cut(s)	1181
SpeI	ACTAGT	1 cut(s)	137
Sse9I	AATT	7 cut(s)	218, 421, 456, 721, 801, 847, 981
SsiI	CCGC	2 cut(s)	31, 391
SspI	AATATT	2 cut(s)	272, 654
SspMI	CTAG	3 cut(s)	138, 377, 875
StyD4I	CCNGG	1 cut(s)	343
StyI	CCWWGG	1 cut(s)	492
TaaI	ACNGT	7 cut(s)	134, 158, 754, 782, 958, 1069, 1259
TaiI	ACGT	2 cut(s)	417, 595
TaqI	TCGA	6 cut(s)	99, 660, 670, 834, 1062, 1182
TasI	AATT	7 cut(s)	218, 421, 456, 721, 801, 847, 981
TatI	WGTACW	2 cut(s)	255, 970
TauI	GCSGC	1 cut(s)	33
TfiI	GAWTC	2 cut(s)	206, 497
Tru1I	TTAA	4 cut(s)	105, 239, 792, 939
Tru9I	TTAA	4 cut(s)	105, 239, 792, 939
TscAI	CASTG	8 cut(s)	40, 163, 313, 544, 609, 757, 1074, 1264
TseFI	GTSAC	4 cut(s)	51, 143, 687, 748
TseI	GCWGC	5 cut(s)	118, 373, 437, 863, 866
Tsp45I	GTSAC	4 cut(s)	51, 143, 687, 748
TspDTI	ATGAA	6 cut(s)	231, 383, 392, 566, 719, 1059
TspGWI	ACGGA	2 cut(s)	170, 371
TspRI	CASTG	8 cut(s)	40, 163, 313, 544, 609, 757, 1074, 1264
Tth111I	GACNNNGTC	1 cut(s)	1115
XapI	RAATTY	1 cut(s)	218
XceI	RCATGY	2 cut(s)	977, 1135
XhoI	CTCGAG	1 cut(s)	1181
XmiI	GTMKAC	1 cut(s)	777
XmnI	GAANNNNTTC	2 cut(s)	42, 291
XspI	CTAG	3 cut(s)	138, 377, 875
Zsp2I	ATGCAT	1 cut(s)	409

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.