FvH4_3g21550

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0016103
Physical Location & Seq
Forward (+)
14648387 .. 14650085
1699 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g21550.t1

Sequence Viewer

Length: 573 bp
ATGAAGTTCTTCTTGATTTCCTGCTGTGGTGTCACCATGACCGCGCGTACGACGTCCCGAGCCGGTTCTTCTCCCTCTATGTCGCCTTCCGGAAGGAAGAAGATGGTTTCCCCCGCTCGCGGTGAGGTATCGTCCCCTGCGCGCGGACGGGTGTCGCCTTCGTCACCCGGGAGCGGAAAGCATTTGCCCTCGTCCCCCGGACGGCGGAGTGCAAAGTCGCCGCGGGGATTTTCGAATCCGCGCGTTATTCCTGTGAGACAGACGAGGTCTGAGCTAGGGTTGGCACCGGCACCTCAGCGGCCACCGCGGCGGAAGAGCGGGAGGCCGGTGGGGTCGGCGGCTTCAGCCTTGCAGTATTCCGAGTGGACGCCGTCGCTGTGCACGATCGCCGAGGACAACGTCGTTGTGTCGAGGAGATCCCTTCGGGATCGGACGCCCAGCATTGAGCGAGCGGTTAACGAAAAGAGTGGTAGAGAAATGTCGCGAGCTCAAAGCCGCAGCCACACCGATGATTACGGGCGAGCTTCAGTCACAGTTATTCCGGCTTATGCTCCGACGCCGTTCTTGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

191

Amino Acids

20.41

Weight (kDa)

11.61

Isoelectric Point (pI)

90.03

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016103)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G54585
fragaria_vesca FvH4_3g21550
malus_domestica MD03G1215100.v1.1 MD11G1229800.v1.1
prunus_persica Prupe.4G197700_v2.0.a1
pyrus_communis pycom03g16360 pycom11g20350
rosa_laevigata RLG00000033742
rosa_roxburghii Rroxscaffold_1G00043960
rosa_rugosa Rorug05G0159700
rosa_samantha Rh5AG251800 Rh5BG253300 Rh5CG286000 Rh5DG261900
rosa_wichuraiana Rw5G023190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 56
AccB1I GGYRCC 2 cut(s) 283, 289
AccBSI CCGCTC 4 cut(s) 116, 174, 318, 452
AccIII TCCGGA 1 cut(s) 89
AclWI GGATC 2 cut(s) 411, 435
AcoI YGGCCR 1 cut(s) 299
AcuI CTGAAG 2 cut(s) 327, 510
AcyI GRCGYC 4 cut(s) 53, 368, 434, 557
AfaI GTAC 1 cut(s) 49
AfiI CCNNNNNNNGG 5 cut(s) 119, 143, 173, 201, 204
AluBI AGCT 3 cut(s) 274, 488, 524
AluI AGCT 3 cut(s) 274, 488, 524
Alw21I GWGCWC 2 cut(s) 383, 490
Alw26I GTCTC 1 cut(s) 250
Alw44I GTGCAC 1 cut(s) 379
AlwI GGATC 2 cut(s) 411, 435
Ama87I CYCGRG 2 cut(s) 57, 167
Aor13HI TCCGGA 1 cut(s) 89
AoxI GGCC 2 cut(s) 299, 323
ApaLI GTGCAC 1 cut(s) 379
ApeKI GCWGC 1 cut(s) 498
Asp700I GAANNNNTTC 1 cut(s) 8
AspLEI GCGC 4 cut(s) 46, 142, 144, 243
AsuC2I CCSGG 3 cut(s) 168, 169, 198
AsuHPI GGTGA 3 cut(s) 25, 134, 156
AsuII TTCGAA 1 cut(s) 233
AvaI CYCGRG 2 cut(s) 57, 167
BaeGI GKGCMC 1 cut(s) 383
BanI GGYRCC 2 cut(s) 283, 289
BanII GRGCYC 1 cut(s) 490
Bbv12I GWGCWC 2 cut(s) 383, 490
BbvCI CCTCAGC 1 cut(s) 294
BbvI GCAGC 1 cut(s) 510
BccI CCATC 1 cut(s) 97
BceAI ACGGC 3 cut(s) 218, 355, 544
BcnI CCSGG 3 cut(s) 168, 169, 198
BcoDI GTCTC 1 cut(s) 250
BfaI CTAG 1 cut(s) 275
BglI GCCNNNNNGGC 1 cut(s) 307
BisI GCNGC 6 cut(s) 221, 299, 308, 339, 496, 499
BlsI GCNGC 6 cut(s) 222, 300, 309, 340, 497, 500
Bme1390I CCNGG 3 cut(s) 168, 169, 198
BmeT110I CYCGRG 2 cut(s) 57, 167
BmiI GGNNCC 2 cut(s) 285, 291
BmrFI CCNGG 3 cut(s) 168, 169, 198
BoxI GACNNNNGTC 1 cut(s) 151
Bpu10I CCTNAGC 1 cut(s) 294
Bpu14I TTCGAA 1 cut(s) 233
BpuMI CCSGG 3 cut(s) 168, 169, 198
BsaHI GRCGYC 4 cut(s) 53, 368, 434, 557
BsaJI CCNNGG 5 cut(s) 167, 196, 221, 305, 390
BsaWI WCCGGW 1 cut(s) 89
Bsc4I CCNNNNNNNGG 5 cut(s) 119, 143, 173, 201, 204
Bse118I RCCGGY 3 cut(s) 62, 286, 325
BseAI TCCGGA 1 cut(s) 89
BseDI CCNNGG 5 cut(s) 167, 196, 221, 305, 390
BseLI CCNNNNNNNGG 5 cut(s) 119, 143, 173, 201, 204
BseMII CTCAG 2 cut(s) 261, 308
BsePI GCGCGC 1 cut(s) 140
BseRI GAGGAG 1 cut(s) 427
BseSI GKGCMC 1 cut(s) 383
BseXI GCAGC 1 cut(s) 510
BseYI CCCAGC 1 cut(s) 437
Bsh1285I CGRYCG 1 cut(s) 387
BshFI GGCC 2 cut(s) 301, 325
BshNI GGYRCC 2 cut(s) 283, 289
BsiEI CGRYCG 1 cut(s) 387
BsiHKAI GWGCWC 2 cut(s) 383, 490
BsiHKCI CYCGRG 2 cut(s) 57, 167
BsiSI CCGG 7 cut(s) 63, 90, 168, 198, 287, 326, 542
BsiWI CGTACG 1 cut(s) 47
BslFI GGGAC 3 cut(s) 40, 118, 178
BslI CCNNNNNNNGG 5 cut(s) 119, 143, 173, 201, 204
BsmAI GTCTC 1 cut(s) 250
BsmFI GGGAC 3 cut(s) 40, 118, 178
BsnI GGCC 2 cut(s) 301, 325
BsoBI CYCGRG 2 cut(s) 57, 167
Bsp119I TTCGAA 1 cut(s) 233
Bsp1286I GDGCHC 2 cut(s) 383, 490
Bsp13I TCCGGA 1 cut(s) 89
Bsp143I GATC 3 cut(s) 384, 416, 427
Bsp68I TCGCGA 1 cut(s) 484
BspANI GGCC 2 cut(s) 301, 325
BspCNI CTCAG 2 cut(s) 262, 307
BspEI TCCGGA 1 cut(s) 89
BspLI GGNNCC 2 cut(s) 285, 291
BspPI GGATC 2 cut(s) 411, 435
BspQI GCTCTTC 1 cut(s) 308
BspT104I TTCGAA 1 cut(s) 233
BspT107I GGYRCC 2 cut(s) 283, 289
BsrBI CCGCTC 4 cut(s) 116, 174, 318, 452
BsrFI RCCGGY 3 cut(s) 62, 286, 325
BssAI RCCGGY 3 cut(s) 62, 286, 325
BssECI CCNNGG 5 cut(s) 167, 196, 221, 305, 390
BssHII GCGCGC 1 cut(s) 140
BssMI GATC 3 cut(s) 384, 416, 427
BssNI GRCGYC 4 cut(s) 53, 368, 434, 557
Bst4CI ACNGT 1 cut(s) 535
Bst6I CTCTTC 1 cut(s) 308
BstACI GRCGYC 4 cut(s) 53, 368, 434, 557
BstBI TTCGAA 1 cut(s) 233
BstC8I GCNNGC 5 cut(s) 118, 142, 450, 486, 522
BstDEI CTNAG 2 cut(s) 270, 294
BstDSI CCRYGG 2 cut(s) 221, 305
BstHHI GCGC 4 cut(s) 46, 142, 144, 243
BstKTI GATC 3 cut(s) 387, 419, 430
BstMAI GTCTC 1 cut(s) 250
BstMBI GATC 3 cut(s) 384, 416, 427
BstMCI CGRYCG 1 cut(s) 387
BstMWI GCNNNNNNNGC 3 cut(s) 304, 307, 344
BstPAI GACNNNNGTC 1 cut(s) 151
BstSCI CCNGG 3 cut(s) 166, 167, 196
BstSLI GKGCMC 1 cut(s) 383
BstV1I GCAGC 1 cut(s) 510
BstX2I RGATCY 1 cut(s) 416
BstYI RGATCY 1 cut(s) 416
BsuRI GGCC 2 cut(s) 301, 325
BtgI CCRYGG 2 cut(s) 221, 305
BtuMI TCGCGA 1 cut(s) 484
Cac8I GCNNGC 5 cut(s) 118, 142, 450, 486, 522
CfoI GCGC 4 cut(s) 46, 142, 144, 243
Cfr10I RCCGGY 3 cut(s) 62, 286, 325
Cfr42I CCGCGG 2 cut(s) 224, 308
Cfr9I CCCGGG 1 cut(s) 167
CseI GACGC 3 cut(s) 376, 442, 565
Csp6I GTAC 1 cut(s) 48
CviAII CATG 1 cut(s) 37
CviQI GTAC 1 cut(s) 48
DdeI CTNAG 2 cut(s) 270, 294
DpnI GATC 3 cut(s) 386, 418, 429
DpnII GATC 3 cut(s) 384, 416, 427
EaeI YGGCCR 1 cut(s) 299
Eam1104I CTCTTC 1 cut(s) 308
EarI CTCTTC 1 cut(s) 308
EciI GGCGGA 2 cut(s) 220, 325
Ecl136II GAGCTC 1 cut(s) 488
Eco24I GRGCYC 1 cut(s) 490
Eco53kI GAGCTC 1 cut(s) 488
Eco57I CTGAAG 2 cut(s) 327, 510
Eco88I CYCGRG 2 cut(s) 57, 167
EcoICRI GAGCTC 1 cut(s) 488
EcoT38I GRGCYC 1 cut(s) 490
FaeI CATG 1 cut(s) 40
FaiI YATR 3 cut(s) 38, 80, 549
FalI AAGNNNNNCTT 1 cut(s) 28
FaqI GGGAC 3 cut(s) 40, 118, 178
FatI CATG 1 cut(s) 36
FauI CCCGC 3 cut(s) 121, 216, 311
Fnu4HI GCNGC 6 cut(s) 221, 299, 308, 339, 496, 499
FriOI GRGCYC 1 cut(s) 490
Fsp4HI GCNGC 6 cut(s) 221, 299, 308, 339, 496, 499
FspBI CTAG 1 cut(s) 275
GlaI GCGC 4 cut(s) 45, 141, 143, 242
GluI GCNGC 6 cut(s) 221, 299, 308, 339, 496, 499
GsaI CCCAGC 1 cut(s) 441
HaeIII GGCC 2 cut(s) 301, 325
HapII CCGG 7 cut(s) 63, 90, 168, 198, 287, 326, 542
HgaI GACGC 3 cut(s) 376, 442, 565
HhaI GCGC 4 cut(s) 46, 142, 144, 243
Hin1I GRCGYC 4 cut(s) 53, 368, 434, 557
Hin1II CATG 1 cut(s) 40
Hin6I GCGC 4 cut(s) 44, 140, 142, 241
HinP1I GCGC 4 cut(s) 44, 140, 142, 241
HincII GTYRAC 1 cut(s) 457
HindII GTYRAC 1 cut(s) 457
HinfI GANTC 1 cut(s) 235
HpaI GTTAAC 1 cut(s) 457
HpaII CCGG 7 cut(s) 63, 90, 168, 198, 287, 326, 542
HphI GGTGA 3 cut(s) 25, 134, 156
Hpy166II GTNNAC 3 cut(s) 366, 381, 457
Hpy188I TCNGA 4 cut(s) 271, 361, 432, 555
Hpy188III TCNNGA 5 cut(s) 13, 57, 90, 425, 483
Hpy8I GTNNAC 3 cut(s) 366, 381, 457
Hpy99I CGWCG 4 cut(s) 55, 376, 404, 559
HpyAV CCTTC 4 cut(s) 87, 96, 168, 431
HpyCH4III ACNGT 1 cut(s) 535
HpyCH4IV ACGT 2 cut(s) 53, 399
HpyCH4V TGCA 3 cut(s) 212, 352, 381
HpyF10VI GCNNNNNNNGC 3 cut(s) 304, 307, 344
HpyF3I CTNAG 2 cut(s) 270, 294
HpySE526I ACGT 2 cut(s) 53, 399
Hsp92I GRCGYC 4 cut(s) 53, 368, 434, 557
Hsp92II CATG 1 cut(s) 40
HspAI GCGC 4 cut(s) 44, 140, 142, 241
Kpn2I TCCGGA 1 cut(s) 89
KspAI GTTAAC 1 cut(s) 457
KspI CCGCGG 2 cut(s) 224, 308
Kzo9I GATC 3 cut(s) 384, 416, 427
LguI GCTCTTC 1 cut(s) 308
LmnI GCTCC 2 cut(s) 171, 556
Lsp1109I GCAGC 1 cut(s) 510
MaeI CTAG 1 cut(s) 275
MaeII ACGT 2 cut(s) 53, 399
MaeIII GTNAC 3 cut(s) 31, 162, 529
MalI GATC 3 cut(s) 386, 418, 429
MbiI CCGCTC 4 cut(s) 116, 174, 318, 452
MboI GATC 3 cut(s) 384, 416, 427
MboII GAAGA 4 cut(s) 60, 109, 112, 325
MflI RGATCY 1 cut(s) 416
MhlI GDGCHC 2 cut(s) 383, 490
MnlI CCTC 8 cut(s) 85, 118, 199, 258, 303, 315, 385, 405
MroI TCCGGA 1 cut(s) 89
MroXI GAANNNNTTC 1 cut(s) 8
MseI TTAA 1 cut(s) 456
MslI CAYNNNNRTG 1 cut(s) 507
MspA1I CMGCKG 3 cut(s) 223, 298, 307
MspI CCGG 7 cut(s) 63, 90, 168, 198, 287, 326, 542
MspR9I CCNGG 3 cut(s) 168, 169, 198
MwoI GCNNNNNNNGC 3 cut(s) 304, 307, 344
NciI CCSGG 3 cut(s) 168, 169, 198
NdeII GATC 3 cut(s) 384, 416, 427
NlaIII CATG 1 cut(s) 40
NlaIV GGNNCC 2 cut(s) 285, 291
NmeAIII GCCGAG 1 cut(s) 415
NmuCI GTSAC 3 cut(s) 31, 162, 529
NruI TCGCGA 1 cut(s) 484
NspV TTCGAA 1 cut(s) 233
PauI GCGCGC 1 cut(s) 140
PciSI GCTCTTC 1 cut(s) 308
PcsI WCGNNNNNNNCGW 1 cut(s) 380
PdmI GAANNNNTTC 1 cut(s) 8
PfeI GAWTC 1 cut(s) 235
Pfl23II CGTACG 1 cut(s) 47
PflFI GACNNNGTC 3 cut(s) 265, 370, 398
PkrI GCNGC 6 cut(s) 222, 300, 309, 340, 497, 500
Ple19I CGATCG 1 cut(s) 387
PshAI GACNNNNGTC 1 cut(s) 151
Psp124BI GAGCTC 1 cut(s) 490
PspFI CCCAGC 1 cut(s) 437
PspLI CGTACG 1 cut(s) 47
PspN4I GGNNCC 2 cut(s) 285, 291
PsuI RGATCY 1 cut(s) 416
PsyI GACNNNGTC 3 cut(s) 265, 370, 398
PteI GCGCGC 1 cut(s) 140
PvuI CGATCG 1 cut(s) 387
RruI TCGCGA 1 cut(s) 484
RsaI GTAC 1 cut(s) 49
RsaNI GTAC 1 cut(s) 48
RseI CAYNNNNRTG 1 cut(s) 507
SacI GAGCTC 1 cut(s) 490
SacII CCGCGG 2 cut(s) 224, 308
SapI GCTCTTC 1 cut(s) 308
SaqAI TTAA 1 cut(s) 456
SatI GCNGC 6 cut(s) 221, 299, 308, 339, 496, 499
Sau3AI GATC 3 cut(s) 384, 416, 427
ScrFI CCNGG 3 cut(s) 168, 169, 198
SduI GDGCHC 2 cut(s) 383, 490
SetI ASST 8 cut(s) 56, 129, 269, 276, 295, 402, 490, 526
Sfr303I CCGCGG 2 cut(s) 224, 308
SfuI TTCGAA 1 cut(s) 233
SgrBI CCGCGG 2 cut(s) 224, 308
SmaI CCCGGG 1 cut(s) 169
SmiMI CAYNNNNRTG 1 cut(s) 507
SspMI CTAG 1 cut(s) 275
SstI GAGCTC 1 cut(s) 490
StyD4I CCNGG 3 cut(s) 166, 167, 196
TaaI ACNGT 1 cut(s) 535
TaiI ACGT 2 cut(s) 56, 402
TaqI TCGA 2 cut(s) 233, 410
TauI GCSGC 5 cut(s) 223, 301, 310, 341, 498
TfiI GAWTC 1 cut(s) 235
Tru1I TTAA 1 cut(s) 456
Tru9I TTAA 1 cut(s) 456
TseFI GTSAC 3 cut(s) 31, 162, 529
TseI GCWGC 1 cut(s) 498
Tsp45I GTSAC 3 cut(s) 31, 162, 529
TspDTI ATGAA 1 cut(s) 17
TspMI CCCGGG 1 cut(s) 167
Tth111I GACNNNGTC 3 cut(s) 265, 370, 398
VneI GTGCAC 1 cut(s) 379
XmaI CCCGGG 1 cut(s) 167
XmnI GAANNNNTTC 1 cut(s) 8
XspI CTAG 1 cut(s) 275
ZraI GACGTC 1 cut(s) 54
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.