MD03G1215100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0016103
Physical Location & Seq
Reverse (-)
29551814 .. 29552996
1183 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1215100.v1.1.491

Sequence Viewer

Length: 435 bp
ATGAAGTTCTTGTTAGAGTTCGTCTCGTGCTGCGGGGCCACTCGCCCCGCAACATCCACCAGCGAGCCAGCTTCGCTTTCTGAGGAGACGAGGTCGCTGGTGAGGGCGCCTAGCCGAATGAGACGCTGCAGCAGAAAGCGCAGGAGCTCGGTAAATTCGTGCGATTGGAGGCCGTCGCTAAGCTCAATTTCCGAGGATAACGTTACTGCGATGGTGGTGGATCGAACGGCTGGGATCGAGAGATCCGTGAAGAGGAAGAGCGGTCATGATCGACCAAAAGTTCAGGTCCGCAGCCACGACGAAGATAACGGGTACGCGAGCGTCATTTGCGATTCCGGCGTTCTCTCCGATGCCTTTTATGTTTTGATATCATGGGATGGACTTGCAATAGCAGCCGGTTATTCATGCTCGAAACTAGGTGGGATAATGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

145

Amino Acids

15.75

Weight (kDa)

9.19

Isoelectric Point (pI)

70.48

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016103)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G54585
fragaria_vesca FvH4_3g21550
malus_domestica MD03G1215100.v1.1 MD11G1229800.v1.1
prunus_persica Prupe.4G197700_v2.0.a1
pyrus_communis pycom03g16360 pycom11g20350
rosa_laevigata RLG00000033742
rosa_roxburghii Rroxscaffold_1G00043960
rosa_rugosa Rorug05G0159700
rosa_samantha Rh5AG251800 Rh5BG253300 Rh5CG286000 Rh5DG261900
rosa_wichuraiana Rw5G023190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 106
AccBSI CCGCTC 1 cut(s) 261
AccII CGCG 1 cut(s) 317
AciI CCGC 4 cut(s) 33, 48, 261, 289
AclI AACGTT 1 cut(s) 201
AclWI GGATC 3 cut(s) 228, 237, 242
AcsI RAATTY 1 cut(s) 154
AcyI GRCGYC 1 cut(s) 107
AfaI GTAC 1 cut(s) 314
AfiI CCNNNNNNNGG 1 cut(s) 252
AluBI AGCT 3 cut(s) 71, 147, 183
AluI AGCT 3 cut(s) 71, 147, 183
Alw21I GWGCWC 1 cut(s) 149
Alw26I GTCTC 3 cut(s) 28, 80, 115
AlwI GGATC 3 cut(s) 228, 237, 242
AoxI GGCC 2 cut(s) 36, 170
ApeKI GCWGC 5 cut(s) 30, 126, 129, 291, 392
ApoI RAATTY 1 cut(s) 154
AspLEI GCGC 2 cut(s) 109, 141
AspS9I GGNCC 2 cut(s) 36, 286
AsuHPI GGTGA 1 cut(s) 112
AvaII GGWCC 1 cut(s) 286
BanI GGYRCC 1 cut(s) 106
BanII GRGCYC 1 cut(s) 149
BauI CACGAG 1 cut(s) 25
Bbv12I GWGCWC 1 cut(s) 149
BbvI GCAGC 5 cut(s) 17, 113, 141, 303, 404
BccI CCATC 2 cut(s) 205, 371
BceAI ACGGC 2 cut(s) 157, 243
BcoDI GTCTC 3 cut(s) 28, 80, 115
BfaI CTAG 2 cut(s) 111, 416
BfmI CTRYAG 1 cut(s) 127
BfoI RGCGCY 1 cut(s) 110
BisI GCNGC 5 cut(s) 31, 127, 130, 292, 393
BlpI GCTNAGC 1 cut(s) 179
BlsI GCNGC 5 cut(s) 32, 128, 131, 293, 394
Bme18I GGWCC 1 cut(s) 286
BmgT120I GGNCC 2 cut(s) 36, 286
BmiI GGNNCC 2 cut(s) 37, 108
BmsI GCATC 1 cut(s) 340
BplI GAGNNNNNCTC 2 cut(s) 8, 40
Bpu1102I GCTNAGC 1 cut(s) 179
BsaHI GRCGYC 1 cut(s) 107
BsaJI CCNNGG 1 cut(s) 192
BsaXI ACNNNNNCTCC 2 cut(s) 77, 107
Bsc4I CCNNNNNNNGG 1 cut(s) 252
Bse118I RCCGGY 1 cut(s) 395
BseDI CCNNGG 1 cut(s) 192
BseGI GGATG 2 cut(s) 53, 382
BseLI CCNNNNNNNGG 1 cut(s) 252
BseMII CTCAG 1 cut(s) 72
BseRI GAGGAG 1 cut(s) 98
BseXI GCAGC 5 cut(s) 17, 113, 141, 303, 404
BseYI CCCAGC 1 cut(s) 230
Bsh1236I CGCG 1 cut(s) 317
BshFI GGCC 2 cut(s) 38, 172
BshNI GGYRCC 1 cut(s) 106
BsiHKAI GWGCWC 1 cut(s) 149
BsiSI CCGG 2 cut(s) 336, 396
BslI CCNNNNNNNGG 1 cut(s) 252
BsmAI GTCTC 3 cut(s) 28, 80, 115
BsmBI CGTCTC 3 cut(s) 28, 80, 115
BsnI GGCC 2 cut(s) 38, 172
Bsp1286I GDGCHC 1 cut(s) 149
Bsp143I GATC 4 cut(s) 220, 234, 242, 268
Bsp1720I GCTNAGC 1 cut(s) 179
BspACI CCGC 4 cut(s) 33, 48, 261, 289
BspANI GGCC 2 cut(s) 38, 172
BspCNI CTCAG 1 cut(s) 73
BspFNI CGCG 1 cut(s) 317
BspHI TCATGA 1 cut(s) 265
BspLI GGNNCC 2 cut(s) 37, 108
BspMAI CTGCAG 1 cut(s) 131
BspPI GGATC 3 cut(s) 228, 237, 242
BspQI GCTCTTC 1 cut(s) 251
BspT107I GGYRCC 1 cut(s) 106
BsrBI CCGCTC 1 cut(s) 261
BsrFI RCCGGY 1 cut(s) 395
BssAI RCCGGY 1 cut(s) 395
BssECI CCNNGG 1 cut(s) 192
BssMI GATC 4 cut(s) 220, 234, 242, 268
BssNI GRCGYC 1 cut(s) 107
BssSI CACGAG 1 cut(s) 25
Bst2BI CACGAG 1 cut(s) 25
Bst6I CTCTTC 2 cut(s) 245, 251
BstACI GRCGYC 1 cut(s) 107
BstC8I GCNNGC 3 cut(s) 65, 69, 319
BstDEI CTNAG 2 cut(s) 81, 179
BstF5I GGATG 2 cut(s) 53, 382
BstFNI CGCG 1 cut(s) 317
BstH2I RGCGCY 1 cut(s) 110
BstHHI GCGC 2 cut(s) 109, 141
BstKTI GATC 4 cut(s) 223, 237, 245, 271
BstMAI GTCTC 3 cut(s) 28, 80, 115
BstMBI GATC 4 cut(s) 220, 234, 242, 268
BstMWI GCNNNNNNNGC 5 cut(s) 73, 138, 327, 336, 392
BstSFI CTRYAG 1 cut(s) 127
BstUI CGCG 1 cut(s) 317
BstV1I GCAGC 5 cut(s) 17, 113, 141, 303, 404
BstX2I RGATCY 1 cut(s) 242
BstYI RGATCY 1 cut(s) 242
BsuRI GGCC 2 cut(s) 38, 172
BtgZI GCGATG 1 cut(s) 224
BtsCI GGATG 2 cut(s) 53, 382
Cac8I GCNNGC 3 cut(s) 65, 69, 319
CciI TCATGA 1 cut(s) 265
CfoI GCGC 2 cut(s) 109, 141
Cfr10I RCCGGY 1 cut(s) 395
Cfr13I GGNCC 2 cut(s) 36, 286
CseI GACGC 2 cut(s) 132, 310
Csp6I GTAC 1 cut(s) 313
CviAII CATG 3 cut(s) 266, 372, 405
CviQI GTAC 1 cut(s) 313
DdeI CTNAG 2 cut(s) 81, 179
DinI GGCGCC 1 cut(s) 108
DpnI GATC 4 cut(s) 222, 236, 244, 270
DpnII GATC 4 cut(s) 220, 234, 242, 268
Eam1104I CTCTTC 2 cut(s) 245, 251
EarI CTCTTC 2 cut(s) 245, 251
Ecl136II GAGCTC 1 cut(s) 147
Eco24I GRGCYC 1 cut(s) 149
Eco32I GATATC 1 cut(s) 369
Eco47I GGWCC 1 cut(s) 286
Eco53kI GAGCTC 1 cut(s) 147
EcoICRI GAGCTC 1 cut(s) 147
EcoRV GATATC 1 cut(s) 369
EcoT38I GRGCYC 1 cut(s) 149
EgeI GGCGCC 1 cut(s) 108
EheI GGCGCC 1 cut(s) 108
Esp3I CGTCTC 3 cut(s) 28, 80, 115
FaeI CATG 3 cut(s) 269, 375, 408
FaiI YATR 4 cut(s) 267, 360, 373, 406
FatI CATG 3 cut(s) 265, 371, 404
FauI CCCGC 2 cut(s) 26, 55
Fnu4HI GCNGC 5 cut(s) 31, 127, 130, 292, 393
FokI GGATG 2 cut(s) 40, 389
FriOI GRGCYC 1 cut(s) 149
Fsp4HI GCNGC 5 cut(s) 31, 127, 130, 292, 393
FspBI CTAG 2 cut(s) 111, 416
GlaI GCGC 2 cut(s) 108, 140
GluI GCNGC 5 cut(s) 31, 127, 130, 292, 393
GsaI CCCAGC 1 cut(s) 234
HaeII RGCGCY 1 cut(s) 110
HaeIII GGCC 2 cut(s) 38, 172
HapII CCGG 2 cut(s) 336, 396
HgaI GACGC 2 cut(s) 132, 310
HhaI GCGC 2 cut(s) 109, 141
Hin1I GRCGYC 1 cut(s) 107
Hin1II CATG 3 cut(s) 269, 375, 408
Hin6I GCGC 2 cut(s) 107, 139
HinP1I GCGC 2 cut(s) 107, 139
HinfI GANTC 1 cut(s) 332
HpaII CCGG 2 cut(s) 336, 396
HphI GGTGA 1 cut(s) 112
Hpy188I TCNGA 3 cut(s) 82, 193, 349
Hpy188III TCNNGA 2 cut(s) 238, 266
Hpy99I CGWCG 2 cut(s) 178, 302
HpyCH4IV ACGT 1 cut(s) 201
HpyCH4V TGCA 2 cut(s) 129, 386
HpyF10VI GCNNNNNNNGC 5 cut(s) 73, 138, 327, 336, 392
HpyF3I CTNAG 2 cut(s) 81, 179
HpySE526I ACGT 1 cut(s) 201
Hsp92I GRCGYC 1 cut(s) 107
Hsp92II CATG 3 cut(s) 269, 375, 408
HspAI GCGC 2 cut(s) 107, 139
KasI GGCGCC 1 cut(s) 106
Kzo9I GATC 4 cut(s) 220, 234, 242, 268
LguI GCTCTTC 1 cut(s) 251
LmnI GCTCC 1 cut(s) 144
LpnPI CCDG 8 cut(s) 73, 81, 83, 127, 216, 269, 349, 409
Lsp1109I GCAGC 5 cut(s) 17, 113, 141, 303, 404
LweI GCATC 1 cut(s) 340
MaeI CTAG 2 cut(s) 111, 416
MaeII ACGT 1 cut(s) 201
MaeIII GTNAC 1 cut(s) 202
MalI GATC 4 cut(s) 222, 236, 244, 270
MbiI CCGCTC 1 cut(s) 261
MboI GATC 4 cut(s) 220, 234, 242, 268
MboII GAAGA 3 cut(s) 262, 268, 314
MflI RGATCY 1 cut(s) 242
MhlI GDGCHC 1 cut(s) 149
MluCI AATT 2 cut(s) 154, 186
Mly113I GGCGCC 1 cut(s) 107
MnlI CCTC 6 cut(s) 76, 84, 96, 162, 187, 246
MspI CCGG 2 cut(s) 336, 396
MvnI CGCG 1 cut(s) 317
MwoI GCNNNNNNNGC 5 cut(s) 73, 138, 327, 336, 392
NarI GGCGCC 1 cut(s) 107
NdeII GATC 4 cut(s) 220, 234, 242, 268
NlaIII CATG 3 cut(s) 269, 375, 408
NlaIV GGNNCC 2 cut(s) 37, 108
PagI TCATGA 1 cut(s) 265
PciSI GCTCTTC 1 cut(s) 251
PcsI WCGNNNNNNNCGW 2 cut(s) 155, 243
PfeI GAWTC 1 cut(s) 332
PflFI GACNNNGTC 1 cut(s) 91
PkrI GCNGC 5 cut(s) 32, 128, 131, 293, 394
PluTI GGCGCC 1 cut(s) 110
Psp124BI GAGCTC 1 cut(s) 149
Psp1406I AACGTT 1 cut(s) 201
PspFI CCCAGC 1 cut(s) 230
PspN4I GGNNCC 2 cut(s) 37, 108
PspPI GGNCC 2 cut(s) 36, 286
PstI CTGCAG 1 cut(s) 131
PsuI RGATCY 1 cut(s) 242
PsyI GACNNNGTC 1 cut(s) 91
RsaI GTAC 1 cut(s) 314
RsaNI GTAC 1 cut(s) 313
SacI GAGCTC 1 cut(s) 149
SapI GCTCTTC 1 cut(s) 251
SatI GCNGC 5 cut(s) 31, 127, 130, 292, 393
Sau3AI GATC 4 cut(s) 220, 234, 242, 268
Sau96I GGNCC 2 cut(s) 36, 286
SduI GDGCHC 1 cut(s) 149
SetI ASST 7 cut(s) 73, 95, 149, 185, 204, 288, 421
SfaNI GCATC 1 cut(s) 340
SfcI CTRYAG 1 cut(s) 127
SfoI GGCGCC 1 cut(s) 108
SinI GGWCC 1 cut(s) 286
Sse9I AATT 2 cut(s) 154, 186
SsiI CCGC 4 cut(s) 33, 48, 261, 289
SspDI GGCGCC 1 cut(s) 106
SspMI CTAG 2 cut(s) 111, 416
SstI GAGCTC 1 cut(s) 149
TaiI ACGT 1 cut(s) 204
TaqI TCGA 4 cut(s) 223, 237, 271, 410
TasI AATT 2 cut(s) 154, 186
TfiI GAWTC 1 cut(s) 332
TseI GCWGC 5 cut(s) 30, 126, 129, 291, 392
TspDTI ATGAA 2 cut(s) 17, 393
TspGWI ACGGA 1 cut(s) 235
Tth111I GACNNNGTC 1 cut(s) 91
VpaK11BI GGWCC 1 cut(s) 286
XapI RAATTY 1 cut(s) 154
XspI CTAG 2 cut(s) 111, 416
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.