Rw5G023190

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0016103
Physical Location & Seq
Forward (+)
32672969 .. 32674282
1314 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G023190.1

Sequence Viewer

Length: 591 bp
ATGAGCATGAAGTTCTTCTTGATTTCATGTTGCGGAGTCACGTTGACCGCTCGAGCGACGCGACCCAGTTCTTCTCCTTCTATGTCGCCTTCCGCGCCGGGAAAGAAGATATCTCCCTCGCGCGGAAGAGTGTCGTCTTCGTCTCCCGCACGCGGAAACGGGCGCAGGAAAGTGTCGTCTTCATCCCCCTCGCGCGGAGGGATGTCCTCTACATCTCCCCAGCGGCGGGGGCCAGCAGGGCGCGGATTTTCAAATCCGCGCGTTGTTGCTGTTAGACAGACGAGGTCGGAACTTGGGTTGGCACAGACAGCACAGCGGCTGCCGCGGCGGAAGAGCGGGAGGCCGGTAGGGTCGGCGGCTTCGGCCTTGCCTTATGCTGAGTGGACGCCGTCGCTGTGCACGATCGCCGAGGACAACGTCGTTGTCGTGAGGAGATCCGTTGGGGATCGGACGCCCAGCATTGAGCGAGCGGTTAACGGAAAGAGTGGTAGAGAAATGTCGCGAGTTCAAAGCCGCAGCCACACCGATGATTACGGGCGAGCTTCAGTCACTGTTATTCCGGCGTTTACTCCGACACCGTTCTTGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

196

Amino Acids

20.82

Weight (kDa)

11.87

Isoelectric Point (pI)

82.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016103)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G54585
fragaria_vesca FvH4_3g21550
malus_domestica MD03G1215100.v1.1 MD11G1229800.v1.1
prunus_persica Prupe.4G197700_v2.0.a1
pyrus_communis pycom03g16360 pycom11g20350
rosa_laevigata RLG00000033742
rosa_roxburghii Rroxscaffold_1G00043960
rosa_rugosa Rorug05G0159700
rosa_samantha Rh5AG251800 Rh5BG253300 Rh5CG286000 Rh5DG261900
rosa_wichuraiana Rw5G023190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 3 cut(s) 50, 336, 470
AclWI GGATC 2 cut(s) 429, 453
AcuI CTGAAG 1 cut(s) 528
AcyI GRCGYC 2 cut(s) 386, 452
AfiI CCNNNNNNNGG 5 cut(s) 122, 152, 194, 225, 226
AgsI TTSAA 2 cut(s) 252, 509
AluBI AGCT 1 cut(s) 542
AluI AGCT 1 cut(s) 542
Alw21I GWGCWC 1 cut(s) 401
Alw26I GTCTC 1 cut(s) 147
Alw44I GTGCAC 1 cut(s) 397
AlwI GGATC 2 cut(s) 429, 453
AlwNI CAGNNNCTG 2 cut(s) 319, 551
Ama87I CYCGRG 1 cut(s) 51
AoxI GGCC 3 cut(s) 230, 341, 363
ApaLI GTGCAC 1 cut(s) 397
ApeKI GCWGC 2 cut(s) 319, 516
Asp700I GAANNNNTTC 1 cut(s) 14
AspLEI GCGC 6 cut(s) 97, 123, 165, 195, 243, 261
AspS9I GGNCC 1 cut(s) 230
AsuC2I CCSGG 1 cut(s) 99
AvaI CYCGRG 1 cut(s) 51
BaeGI GKGCMC 1 cut(s) 401
BbsI GAAGAC 2 cut(s) 129, 171
Bbv12I GWGCWC 1 cut(s) 401
BbvI GCAGC 2 cut(s) 306, 528
BceAI ACGGC 1 cut(s) 373
BcnI CCSGG 1 cut(s) 99
BcoDI GTCTC 1 cut(s) 147
BglI GCCNNNNNGGC 1 cut(s) 238
BisI GCNGC 8 cut(s) 224, 317, 320, 323, 326, 357, 514, 517
BlsI GCNGC 8 cut(s) 225, 318, 321, 324, 327, 358, 515, 518
Bme1390I CCNGG 1 cut(s) 99
BmeT110I CYCGRG 1 cut(s) 51
BmgT120I GGNCC 1 cut(s) 230
BmiI GGNNCC 1 cut(s) 231
BmrFI CCNGG 1 cut(s) 99
BmrI ACTGGG 1 cut(s) 60
BmuI ACTGGG 1 cut(s) 60
BpiI GAAGAC 2 cut(s) 129, 171
BpuMI CCSGG 1 cut(s) 99
BsaHI GRCGYC 2 cut(s) 386, 452
BsaJI CCNNGG 2 cut(s) 323, 408
Bsc4I CCNNNNNNNGG 5 cut(s) 122, 152, 194, 225, 226
Bse118I RCCGGY 1 cut(s) 343
Bse1I ACTGG 1 cut(s) 66
BseDI CCNNGG 2 cut(s) 323, 408
BseGI GGATG 2 cut(s) 182, 207
BseLI CCNNNNNNNGG 5 cut(s) 122, 152, 194, 225, 226
BseMII CTCAG 1 cut(s) 369
BseNI ACTGG 1 cut(s) 66
BseRI GAGGAG 1 cut(s) 445
BseSI GKGCMC 1 cut(s) 401
BseXI GCAGC 2 cut(s) 306, 528
BseYI CCCAGC 2 cut(s) 219, 455
Bsh1285I CGRYCG 1 cut(s) 405
BshFI GGCC 3 cut(s) 232, 343, 365
BsiEI CGRYCG 1 cut(s) 405
BsiHKAI GWGCWC 1 cut(s) 401
BsiHKCI CYCGRG 1 cut(s) 51
BsiSI CCGG 3 cut(s) 98, 344, 560
BslI CCNNNNNNNGG 5 cut(s) 122, 152, 194, 225, 226
BsmAI GTCTC 1 cut(s) 147
BsmBI CGTCTC 1 cut(s) 147
BsnI GGCC 3 cut(s) 232, 343, 365
BsoBI CYCGRG 1 cut(s) 51
Bsp1286I GDGCHC 1 cut(s) 401
Bsp143I GATC 3 cut(s) 402, 434, 445
Bsp68I TCGCGA 1 cut(s) 502
BspANI GGCC 3 cut(s) 232, 343, 365
BspCNI CTCAG 1 cut(s) 370
BspLI GGNNCC 1 cut(s) 231
BspPI GGATC 2 cut(s) 429, 453
BspQI GCTCTTC 1 cut(s) 326
BsrBI CCGCTC 3 cut(s) 50, 336, 470
BsrFI RCCGGY 1 cut(s) 343
BsrI ACTGG 1 cut(s) 66
BssAI RCCGGY 1 cut(s) 343
BssECI CCNNGG 2 cut(s) 323, 408
BssMI GATC 3 cut(s) 402, 434, 445
BssNI GRCGYC 2 cut(s) 386, 452
Bst4CI ACNGT 2 cut(s) 553, 579
Bst6I CTCTTC 2 cut(s) 121, 326
BstACI GRCGYC 2 cut(s) 386, 452
BstC8I GCNNGC 4 cut(s) 151, 234, 468, 540
BstDEI CTNAG 1 cut(s) 378
BstDSI CCRYGG 1 cut(s) 323
BstF5I GGATG 2 cut(s) 182, 207
BstHHI GCGC 6 cut(s) 97, 123, 165, 195, 243, 261
BstKTI GATC 3 cut(s) 405, 437, 448
BstMAI GTCTC 1 cut(s) 147
BstMBI GATC 3 cut(s) 402, 434, 445
BstMCI CGRYCG 1 cut(s) 405
BstMWI GCNNNNNNNGC 7 cut(s) 94, 229, 238, 308, 322, 325, 362
BstSCI CCNGG 1 cut(s) 97
BstSLI GKGCMC 1 cut(s) 401
BstV1I GCAGC 2 cut(s) 306, 528
BstV2I GAAGAC 2 cut(s) 129, 171
BstX2I RGATCY 1 cut(s) 434
BstYI RGATCY 1 cut(s) 434
BsuRI GGCC 3 cut(s) 232, 343, 365
BtgI CCRYGG 1 cut(s) 323
BtsCI GGATG 2 cut(s) 182, 207
BtsIMutI CAGTG 1 cut(s) 549
BtuMI TCGCGA 1 cut(s) 502
Cac8I GCNNGC 4 cut(s) 151, 234, 468, 540
CaiI CAGNNNCTG 2 cut(s) 319, 551
CfoI GCGC 6 cut(s) 97, 123, 165, 195, 243, 261
Cfr10I RCCGGY 1 cut(s) 343
Cfr13I GGNCC 1 cut(s) 230
Cfr42I CCGCGG 1 cut(s) 326
CseI GACGC 3 cut(s) 67, 394, 460
CviAII CATG 2 cut(s) 7, 27
CviJI RGCY 8 cut(s) 232, 319, 343, 359, 365, 513, 519, 542
CviKI_1 RGCY 8 cut(s) 232, 319, 343, 359, 365, 513, 519, 542
DdeI CTNAG 1 cut(s) 378
DpnI GATC 3 cut(s) 404, 436, 447
DpnII GATC 3 cut(s) 402, 434, 445
Eam1104I CTCTTC 2 cut(s) 121, 326
EarI CTCTTC 2 cut(s) 121, 326
EciI GGCGGA 1 cut(s) 343
Eco32I GATATC 1 cut(s) 111
Eco57I CTGAAG 1 cut(s) 528
Eco88I CYCGRG 1 cut(s) 51
EcoRV GATATC 1 cut(s) 111
Esp3I CGTCTC 1 cut(s) 147
FaeI CATG 2 cut(s) 10, 30
FaiI YATR 4 cut(s) 8, 28, 83, 375
FalI AAGNNNNNCTT 1 cut(s) 34
FatI CATG 2 cut(s) 6, 26
FauI CCCGC 3 cut(s) 154, 219, 329
Fnu4HI GCNGC 8 cut(s) 224, 317, 320, 323, 326, 357, 514, 517
FokI GGATG 2 cut(s) 169, 214
Fsp4HI GCNGC 8 cut(s) 224, 317, 320, 323, 326, 357, 514, 517
GlaI GCGC 6 cut(s) 96, 122, 164, 194, 242, 260
GluI GCNGC 8 cut(s) 224, 317, 320, 323, 326, 357, 514, 517
GsaI CCCAGC 2 cut(s) 223, 459
HaeIII GGCC 3 cut(s) 232, 343, 365
HapII CCGG 3 cut(s) 98, 344, 560
HgaI GACGC 3 cut(s) 67, 394, 460
HhaI GCGC 6 cut(s) 97, 123, 165, 195, 243, 261
Hin1I GRCGYC 2 cut(s) 386, 452
Hin1II CATG 2 cut(s) 10, 30
Hin6I GCGC 6 cut(s) 95, 121, 163, 193, 241, 259
HinP1I GCGC 6 cut(s) 95, 121, 163, 193, 241, 259
HincII GTYRAC 2 cut(s) 45, 475
HindII GTYRAC 2 cut(s) 45, 475
HinfI GANTC 1 cut(s) 36
HpaI GTTAAC 1 cut(s) 475
HpaII CCGG 3 cut(s) 98, 344, 560
Hpy166II GTNNAC 5 cut(s) 45, 384, 399, 475, 567
Hpy188I TCNGA 3 cut(s) 289, 450, 573
Hpy188III TCNNGA 3 cut(s) 19, 427, 501
Hpy8I GTNNAC 5 cut(s) 45, 384, 399, 475, 567
Hpy99I CGWCG 3 cut(s) 61, 394, 422
HpyAV CCTTC 2 cut(s) 87, 99
HpyCH4III ACNGT 2 cut(s) 553, 579
HpyCH4IV ACGT 2 cut(s) 41, 417
HpyCH4V TGCA 1 cut(s) 399
HpyF10VI GCNNNNNNNGC 7 cut(s) 94, 229, 238, 308, 322, 325, 362
HpyF3I CTNAG 1 cut(s) 378
HpySE526I ACGT 2 cut(s) 41, 417
Hsp92I GRCGYC 2 cut(s) 386, 452
Hsp92II CATG 2 cut(s) 10, 30
HspAI GCGC 6 cut(s) 95, 121, 163, 193, 241, 259
KspAI GTTAAC 1 cut(s) 475
KspI CCGCGG 1 cut(s) 326
Kzo9I GATC 3 cut(s) 402, 434, 445
LguI GCTCTTC 1 cut(s) 326
LpnPI CCDG 9 cut(s) 79, 111, 151, 222, 233, 246, 357, 469, 573
Lsp1109I GCAGC 2 cut(s) 306, 528
MaeII ACGT 2 cut(s) 41, 417
MaeIII GTNAC 2 cut(s) 37, 547
MalI GATC 3 cut(s) 404, 436, 447
MbiI CCGCTC 3 cut(s) 50, 336, 470
MboI GATC 3 cut(s) 402, 434, 445
MboII GAAGA 7 cut(s) 7, 63, 118, 129, 138, 171, 343
MflI RGATCY 1 cut(s) 434
MhlI GDGCHC 1 cut(s) 401
MlyI GAGTC 1 cut(s) 45
MmeI TCCRAC 1 cut(s) 267
MnlI CCTC 8 cut(s) 127, 191, 199, 217, 276, 333, 403, 423
MroXI GAANNNNTTC 1 cut(s) 14
MseI TTAA 1 cut(s) 474
MslI CAYNNNNRTG 1 cut(s) 525
MspA1I CMGCKG 3 cut(s) 223, 316, 325
MspI CCGG 3 cut(s) 98, 344, 560
MspR9I CCNGG 1 cut(s) 99
MwoI GCNNNNNNNGC 7 cut(s) 94, 229, 238, 308, 322, 325, 362
NciI CCSGG 1 cut(s) 99
NdeII GATC 3 cut(s) 402, 434, 445
NlaIII CATG 2 cut(s) 10, 30
NlaIV GGNNCC 1 cut(s) 231
NmeAIII GCCGAG 1 cut(s) 433
NmuCI GTSAC 2 cut(s) 37, 547
NruI TCGCGA 1 cut(s) 502
PaeR7I CTCGAG 1 cut(s) 51
PciSI GCTCTTC 1 cut(s) 326
PcsI WCGNNNNNNNCGW 3 cut(s) 58, 398, 423
PdmI GAANNNNTTC 1 cut(s) 14
PflFI GACNNNGTC 3 cut(s) 283, 388, 416
PkrI GCNGC 8 cut(s) 225, 318, 321, 324, 327, 358, 515, 518
Ple19I CGATCG 1 cut(s) 405
PleI GAGTC 1 cut(s) 44
PpsI GAGTC 1 cut(s) 44
PspFI CCCAGC 2 cut(s) 219, 455
PspN4I GGNNCC 1 cut(s) 231
PspPI GGNCC 1 cut(s) 230
PspXI VCTCGAGB 1 cut(s) 51
PstNI CAGNNNCTG 2 cut(s) 319, 551
PsuI RGATCY 1 cut(s) 434
PsyI GACNNNGTC 3 cut(s) 283, 388, 416
PvuI CGATCG 1 cut(s) 405
RruI TCGCGA 1 cut(s) 502
RseI CAYNNNNRTG 1 cut(s) 525
SacII CCGCGG 1 cut(s) 326
SapI GCTCTTC 1 cut(s) 326
SaqAI TTAA 1 cut(s) 474
SatI GCNGC 8 cut(s) 224, 317, 320, 323, 326, 357, 514, 517
Sau3AI GATC 3 cut(s) 402, 434, 445
Sau96I GGNCC 1 cut(s) 230
SchI GAGTC 1 cut(s) 45
ScrFI CCNGG 1 cut(s) 99
SduI GDGCHC 1 cut(s) 401
SetI ASST 4 cut(s) 44, 287, 420, 544
Sfr274I CTCGAG 1 cut(s) 51
Sfr303I CCGCGG 1 cut(s) 326
SgrBI CCGCGG 1 cut(s) 326
SlaI CTCGAG 1 cut(s) 51
SmiMI CAYNNNNRTG 1 cut(s) 525
SmlI CTYRAG 1 cut(s) 51
SmoI CTYRAG 1 cut(s) 51
StyD4I CCNGG 1 cut(s) 97
TaaI ACNGT 2 cut(s) 553, 579
TaiI ACGT 2 cut(s) 44, 420
TaqI TCGA 1 cut(s) 52
TauI GCSGC 6 cut(s) 226, 319, 325, 328, 359, 516
Tru1I TTAA 1 cut(s) 474
Tru9I TTAA 1 cut(s) 474
TscAI CASTG 1 cut(s) 556
TseFI GTSAC 2 cut(s) 37, 547
TseI GCWGC 2 cut(s) 319, 516
Tsp45I GTSAC 2 cut(s) 37, 547
TspDTI ATGAA 3 cut(s) 15, 23, 171
TspGWI ACGGA 2 cut(s) 427, 492
TspRI CASTG 1 cut(s) 556
Tth111I GACNNNGTC 3 cut(s) 283, 388, 416
VneI GTGCAC 1 cut(s) 397
XhoI CTCGAG 1 cut(s) 51
XmnI GAANNNNTTC 1 cut(s) 14
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.