Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb3

Orthogroup ID

OG0004829

Physical Location & Seq

Genomic Coordinates Reverse (-)

19014839 .. 19026450

Gene Structure 11612 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_3g26140.t4

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2661 bp

ATGGAGAGCAGCGCTGGTCTCCCTGAAGATCTCTCCGATTCTACTCAGCGTCCTGACTCCGACTCCGAGCAGCGAGTTTACTTCGTTCCTTACAGTTGGTGGAGGGATGCGCAAGATTCAGTTGTGGGCGATTCGGATTCAGATAGGGGCAAGAAAGGGATTTTGTGTGTGGCGTCCCCGGCCGCTTCGGCTTATGGTGGCCCTATGAAGATCATCAACAACATTTTCAACTCAGATCTTGTCTTCAATCTCCGGAGAGAGGATGATGCTGCTCAGAATGGCGAAAATGGTGAGGTGGGTGTTTCGGGACGGGACTATGCGTTGGTCCCCGGAGAAATGTGGGTTCAGGCCCTTAGATGGCATAGTGACCCTAAAGCTGCAGTTAAGGATGGTAGGAGTTTTTCGGCTGCAGAAGATGACAAGACTGATGTCTATCCCCTACAACTCAGGCTTTCTGTTCCACGAGAAGCAAATTTGTTGGGAGTAAGAATAACCAAAAAGGATAATGCAGTGGAGTCCTTCAAAAGAGCATGCAAAATCTTCAGTTTGGAGTCTGACATGTTACGCATCTGGGACTTTTCCGGCCAGACTAACTTATTTTTTACTAATGACAAAAGCAAGTTCCCAAGAGACTTGCAACGGCAGTCAGATGAGATCCTACTGGAGTTGCAAGTTTATGGATTGTCAGATTCAATCAAGTGCAGGGAAGGGAAAAAAGATGAGATGGCCATGCAACATTCTACAACTGGAAATTCTTGTGGTGCTTCAGTTATGATGAATGGTTCTGCTGGCAGTACACATTCAAACTTCATTCGTTCATTCTGGGGAACCTCTTGCGAAGCTGGCTCTTTAGGGTTGACAGGATTGCAAAACCTCGGAAATACTTGTTTCATGAATAGCTCCATCCAGTGCTTAGCCCATACACCAAAGCTTGTTGACTATTTTCTTGGAGATTATGCTAGAGAAATAAATAAGGAAAACCCATTGGGCATGGATGGGGAGATTGCTTTAGCATTTGGAGATCTGTTAAGGAAGTTATGGGCTCCTGGAGCAACTCCTGTGGCTCCAAGGACCTTTAAATCAAAACTTGCTCGCTTTGCTCCTCAGTTTAATGGCTTCAATCAACATGATTCACAACAGGAACTCCTGGCGTTTTTGTTGGATGGACTTCATGAAGATCTTAATCGTGTAAAATGCAAGCCTTATGTAGAAGTCAAGGATGGTGATGATCGACCAGATGAAGAGGTAGCAGATGAATATTGGCGAAATCATTTAGCCCGCAATGACTCTATCATTGTTGATGTGTGCCAAGGTCAATATAAATCAACTTTAGTTTGTCCTGTTTGCCAAAAGGTCTCTGTTACATTTGATCCGTTTATGTACCTGTCACTACCATTACCTTCGACAATGATGCGGACCATGACTTTAACAGTTATCAACACTGATGGAATTTCGAAGCCATCTGAACATACCATTAATGTGCCCAAGCATGGAAAGTTTGAAGATCTCATCCGGGGTCTGGGTGTTGCATGCTCACTTGGGGTTGATGAAACCTTTTTAGTGGCCGAGATATACAATAATCGCATTATACGCTATCTTGAAGAGCCCGCTGATGCGTTATCCTTAATTAGAGATGGTGACCAGCTGGTTGCTTATCGGTTTATGAAAAATGTTGAGGAAGTACCTCTGGTTGTCTTCATGCATCAGCAAATGGAGCAGGACATGCATGGGAAATTGAGTTCAAGCTGGAAGCCGTTTGGAATTCCTCTTGTATCAAGACTCTGTAGTTCTGCAACTGGATCTGATATCTGCAATCAGTACTTAAAATTAGTTAAACCATTTGAAATACCTGGAAAAGATTCCGAAGCACATGTTCATAATTCACAGGGCACCGCTCCTATAGAAGTCACTGAATCAGAGGAAACCGCACCTCCTGTTTCAGGTGGGGATGTGAACCTCTGTTATGAAATTGGGGCTGAGTCACTGTTGGATTGTGAATTTCAGTTTTACATTACAGATGAAAAAGGAATTCAAAAGGAGAAAAAAATAGTAATGAATGAGATAGTTGAGAGGAAAATGCCCGAGCATTTATATGTTGCTGTATGTTGGCCAGAAAAATATGTCGAGCAGTATGATACTCGCGTTCTGAGCTCATTGCCATTGGTTTTCAAGCCTGGCCTTTTTTCAAAAAAGCCTCAGGAGACTGTTTCTCTCTATAAGTGCCTTGAAGCATTCTTGAAGGAGGAGCCTCTTGGGCCAGAAGACATGTGGTATTGTCCTGGCTGCAAAAAGCATTGCCAAGCGAGTAAGAAGTTGGATCTTTGGAGACTGCCTGAGATCTTAGTCATTCATTTAAAGCGGTTCTCATATAGTCGATTCTTCAAGAACAAGCTAGAGACATATGTTGACTTCCCTGTTGATAATCTTGATTTGTCAACATACATTGCTTACACGACGGGTGAATTAAGCAATCGTTACATGCTGTATGCAGTTAGTAATCACTTTGGAAGCATGGGTGGCGGTCATTATACTGCATTTGTCCATCATGGGGGCGATCGGTGGTATGACTTTGATGATAGTCATGTTAGCCCTGTGAGCCAGGACAAGATAAAGTCTGCTGCTGCGTATGTTCTTTTCTATAGGAGAGTAGTAGAAGTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko04137 map04137

Pfam Domains

Protein Families

UCH

Protein Analysis

887

Amino Acids

99.69

Weight (kDa)

5.34

Isoelectric Point (pI)

40.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ubiquitin_UBP8	PF25242	149 - 203	4.9e-29	UBP8-like, ubiquitin-like domain
UCH	PF00443	287 - 879	3.3e-74	Ubiquitin carboxyl-terminal hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004829)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G22030 AT5G22030
fragaria_vesca	FvH4_3g26140 FvH4_3g26140 FvH4_3g26140 FvH4_3g26140 FvH4_3g26140 FvH4_3g26140 FvH4_3g26140
malus_domestica	MD03G1159600.v1.1 MD11G1174900.v1.1
prunus_persica	Prupe.6G147300_v2.0.a1 Prupe.6G147300_v2.0.a1 Prupe.6G147300_v2.0.a1 Prupe.6G147300_v2.0.a1
pyrus_communis	pycom03g11220 pycom03g11230 pycom11g14960 pycom11g14970
rosa_chinensis	RchiOBHm_Chr5g0049121
rosa_laevigata	RLG00000034590
rosa_multiflora	Rmu_sc0000530.1_g000038 Rmu_sc0000530.1_g000039
rosa_roxburghii	Rroxscaffold_1G00032700
rosa_rugosa	Rorug05G0243300 Rorug05G0243300
rosa_samantha	Rh5AG322700 Rh5BG333300 Rh5CG358200 Rh5DG344700
rosa_wichuraiana	Rw5G030460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	111
AccB1I	GGYRCC	1 cut(s)	1889
AccBSI	CCGCTC	1 cut(s)	1895
AccII	CGCG	1 cut(s)	2142
AccIII	TCCGGA	1 cut(s)	252
AciI	CCGC	8 cut(s)	183, 1279, 1414, 1608, 1893, 1926, 2359, 2520
AclWI	GGATC	4 cut(s)	649, 1364, 1807, 2325
AcoI	YGGCCR	5 cut(s)	180, 583, 726, 1563, 2108
AcsI	RAATTY	6 cut(s)	472, 751, 1449, 1761, 1997, 2028
AcuI	CTGAAG	3 cut(s)	45, 526, 750
AcyI	GRCGYC	1 cut(s)	173
AfaI	GTAC	4 cut(s)	796, 1382, 1683, 1820
AfeI	AGCGCT	1 cut(s)	13
AfiI	CCNNNNNNNGG	6 cut(s)	259, 357, 1490, 1519, 1940, 2548
AflIII	ACRYGT	3 cut(s)	558, 1870, 2265
AjnI	CCWGG	6 cut(s)	1045, 1146, 1849, 2173, 2278, 2598
AloI	GAACNNNNNNTCC	2 cut(s)	1723, 1755
AluBI	AGCT	8 cut(s)	377, 842, 900, 931, 1645, 1746, 2151, 2392
AluI	AGCT	8 cut(s)	377, 842, 900, 931, 1645, 1746, 2151, 2392
Alw21I	GWGCWC	1 cut(s)	2153
Alw26I	GTCTC	6 cut(s)	23, 624, 1360, 2195, 2320, 2390
AlwI	GGATC	4 cut(s)	649, 1364, 1807, 2325
Ama87I	CYCGRG	1 cut(s)	2081
Aor13HI	TCCGGA	1 cut(s)	252
Aor51HI	AGCGCT	1 cut(s)	13
AoxI	GGCC	9 cut(s)	180, 199, 348, 583, 726, 1563, 2108, 2176, 2255
ApeKI	GCWGC	8 cut(s)	9, 70, 269, 377, 407, 2283, 2618, 2621
ApoI	RAATTY	6 cut(s)	472, 751, 1449, 1761, 1997, 2028
AseI	ATTAAT	1 cut(s)	1476
Asp700I	GAANNNNTTC	1 cut(s)	1858
AspLEI	GCGC	2 cut(s)	14, 112
AspS9I	GGNCC	6 cut(s)	200, 325, 349, 1071, 1416, 2255
AsuC2I	CCSGG	3 cut(s)	179, 330, 1514
AsuHPI	GGTGA	4 cut(s)	302, 1235, 1649, 2471
AsuII	TTCGAA	1 cut(s)	1454
AvaI	CYCGRG	1 cut(s)	2081
AvaII	GGWCC	3 cut(s)	325, 1071, 1416
AxyI	CCTNAGG	1 cut(s)	2196
BaeGI	GKGCMC	2 cut(s)	1485, 1892
BaeI	ACNNNNGTAYC	2 cut(s)	2127, 2160
BalI	TGGCCA	2 cut(s)	728, 2110
BanI	GGYRCC	1 cut(s)	1889
BanII	GRGCYC	3 cut(s)	1045, 1608, 2153
BauI	CACGAG	1 cut(s)	462
BbsI	GAAGAC	3 cut(s)	235, 1687, 2268
Bbv12I	GWGCWC	1 cut(s)	2153
BbvI	GCAGC	8 cut(s)	21, 82, 256, 364, 394, 2270, 2605, 2608
BceAI	ACGGC	2 cut(s)	656, 1738
BcgI	CGANNNNNNTGC	2 cut(s)	1393, 1427
BciT130I	CCWGG	6 cut(s)	1047, 1148, 1851, 2175, 2280, 2600
BcnI	CCSGG	3 cut(s)	179, 330, 1514
BcoDI	GTCTC	6 cut(s)	23, 624, 1360, 2195, 2320, 2390
BfaI	CTAG	2 cut(s)	960, 2393
BfmI	CTRYAG	5 cut(s)	378, 408, 1783, 1899, 2638
BfoI	RGCGCY	1 cut(s)	15
BglI	GCCNNNNNGGC	2 cut(s)	188, 2254
BglII	AGATCT	6 cut(s)	28, 235, 1021, 1177, 1504, 2337
BisI	GCNGC	9 cut(s)	10, 71, 183, 270, 378, 408, 2284, 2619, 2622
BlpI	GCTNAGC	1 cut(s)	913
BlsI	GCNGC	9 cut(s)	11, 72, 184, 271, 379, 409, 2285, 2620, 2623
BmcAI	AGTACT	1 cut(s)	1820
Bme1390I	CCNGG	9 cut(s)	179, 330, 1047, 1148, 1514, 1851, 2175, 2280, 2600
Bme18I	GGWCC	3 cut(s)	325, 1071, 1416
BmeT110I	CYCGRG	1 cut(s)	2081
BmgT120I	GGNCC	6 cut(s)	200, 325, 349, 1071, 1416, 2255
BmiI	GGNNCC	6 cut(s)	327, 829, 1044, 1065, 1891, 2247
BmrFI	CCNGG	9 cut(s)	179, 330, 1047, 1148, 1514, 1851, 2175, 2280, 2600
BmsI	GCATC	6 cut(s)	97, 256, 576, 1401, 1603, 1711
BoxI	GACNNNNGTC	1 cut(s)	428
BpiI	GAAGAC	3 cut(s)	235, 1687, 2268
BpmI	CTGGAG	2 cut(s)	683, 1068
Bpu1102I	GCTNAGC	1 cut(s)	913
Bpu14I	TTCGAA	1 cut(s)	1454
BpuMI	CCSGG	3 cut(s)	179, 330, 1514
BsaBI	GATNNNNATC	2 cut(s)	432, 1182
BsaHI	GRCGYC	1 cut(s)	173
BsaI	GGTCTC	2 cut(s)	23, 1360
BsaJI	CCNNGG	6 cut(s)	177, 328, 874, 1067, 1309, 1513
BsaWI	WCCGGW	1 cut(s)	252
BsaXI	ACNNNNNCTCC	6 cut(s)	47, 77, 1128, 1158, 1915, 1945
Bsc4I	CCNNNNNNNGG	6 cut(s)	259, 357, 1490, 1519, 1940, 2548
Bse1I	ACTGG	4 cut(s)	666, 751, 907, 1801
Bse21I	CCTNAGG	1 cut(s)	2196
Bse3DI	GCAATG	4 cut(s)	1288, 2153, 2293, 2442
Bse8I	GATNNNNATC	2 cut(s)	432, 1182
BseAI	TCCGGA	1 cut(s)	252
BseBI	CCWGG	6 cut(s)	1047, 1148, 1851, 2175, 2280, 2600
BseDI	CCNNGG	6 cut(s)	177, 328, 874, 1067, 1309, 1513
BseGI	GGATG	9 cut(s)	112, 268, 394, 903, 1000, 1168, 1225, 1509, 1954
BseJI	GATNNNNATC	2 cut(s)	432, 1182
BseLI	CCNNNNNNNGG	6 cut(s)	259, 357, 1490, 1519, 1940, 2548
BseMI	GCAATG	4 cut(s)	1288, 2153, 2293, 2442
BseMII	CTCAG	9 cut(s)	59, 246, 287, 460, 1118, 1968, 2138, 2210, 2325
BseNI	ACTGG	4 cut(s)	666, 751, 907, 1801
BseRI	GAGGAG	2 cut(s)	1092, 2258
BseSI	GKGCMC	2 cut(s)	1485, 1892
BseX3I	CGGCCG	1 cut(s)	180
BseXI	GCAGC	8 cut(s)	21, 82, 256, 364, 394, 2270, 2605, 2608
BsgI	GTGCAG	1 cut(s)	721
Bsh1236I	CGCG	1 cut(s)	2142
Bsh1285I	CGRYCG	2 cut(s)	183, 2557
BshFI	GGCC	9 cut(s)	182, 201, 350, 585, 728, 1565, 2110, 2178, 2257
BshNI	GGYRCC	1 cut(s)	1889
BsiEI	CGRYCG	2 cut(s)	183, 2557
BsiHKAI	GWGCWC	1 cut(s)	2153
BsiHKCI	CYCGRG	1 cut(s)	2081
BsiSI	CCGG	5 cut(s)	179, 253, 330, 582, 1513
BslFI	GGGAC	5 cut(s)	160, 311, 321, 326, 587
BslI	CCNNNNNNNGG	6 cut(s)	259, 357, 1490, 1519, 1940, 2548
BsmAI	GTCTC	6 cut(s)	23, 624, 1360, 2195, 2320, 2390
BsmFI	GGGAC	5 cut(s)	160, 311, 321, 326, 587
BsmI	GAATGC	1 cut(s)	2231
BsnI	GGCC	9 cut(s)	182, 201, 350, 585, 728, 1565, 2110, 2178, 2257
Bso31I	GGTCTC	2 cut(s)	23, 1360
BsoBI	CYCGRG	1 cut(s)	2081
Bsp119I	TTCGAA	1 cut(s)	1454
Bsp1286I	GDGCHC	5 cut(s)	1045, 1485, 1608, 1892, 2153
Bsp13I	TCCGGA	1 cut(s)	252
Bsp1720I	GCTNAGC	1 cut(s)	913
BspACI	CCGC	8 cut(s)	183, 1279, 1414, 1608, 1893, 1926, 2359, 2520
BspANI	GGCC	9 cut(s)	182, 201, 350, 585, 728, 1565, 2110, 2178, 2257
BspCNI	CTCAG	9 cut(s)	58, 245, 286, 459, 1117, 1969, 2139, 2209, 2326
BspEI	TCCGGA	1 cut(s)	252
BspFNI	CGCG	1 cut(s)	2142
BspHI	TCATGA	2 cut(s)	891, 1171
BspLI	GGNNCC	6 cut(s)	327, 829, 1044, 1065, 1891, 2247
BspMAI	CTGCAG	2 cut(s)	382, 412
BspPI	GGATC	4 cut(s)	649, 1364, 1807, 2325
BspQI	GCTCTTC	1 cut(s)	1596
BspT104I	TTCGAA	1 cut(s)	1454
BspT107I	GGYRCC	1 cut(s)	1889
BspTNI	GGTCTC	2 cut(s)	23, 1360
BsrBI	CCGCTC	1 cut(s)	1895
BsrDI	GCAATG	4 cut(s)	1288, 2153, 2293, 2442
BsrI	ACTGG	4 cut(s)	666, 751, 907, 1801
BssECI	CCNNGG	6 cut(s)	177, 328, 874, 1067, 1309, 1513
BssNI	GRCGYC	1 cut(s)	173
BssSI	CACGAG	1 cut(s)	462
BssT1I	CCWWGG	2 cut(s)	1067, 1309
Bst2BI	CACGAG	1 cut(s)	462
Bst2UI	CCWGG	6 cut(s)	1047, 1148, 1851, 2175, 2280, 2600
Bst4CI	ACNGT	4 cut(s)	95, 1432, 1986, 2206
Bst6I	CTCTTC	2 cut(s)	1236, 1596
BstACI	GRCGYC	1 cut(s)	173
BstAPI	GCANNNNNTGC	1 cut(s)	1723
BstBI	TTCGAA	1 cut(s)	1454
BstC8I	GCNNGC	8 cut(s)	532, 790, 844, 1093, 1199, 1279, 1531, 1608
BstEII	GGTNACC	1 cut(s)	1637
BstENI	CCTNNNNNAGG	1 cut(s)	1938
BstF5I	GGATG	9 cut(s)	112, 268, 394, 903, 1000, 1168, 1225, 1509, 1954
BstFNI	CGCG	1 cut(s)	2142
BstH2I	RGCGCY	1 cut(s)	15
BstHHI	GCGC	2 cut(s)	14, 112
BstMAI	GTCTC	6 cut(s)	23, 624, 1360, 2195, 2320, 2390
BstMCI	CGRYCG	2 cut(s)	183, 2557
BstNI	CCWGG	6 cut(s)	1047, 1148, 1851, 2175, 2280, 2600
BstNSI	RCATGY	7 cut(s)	534, 562, 1533, 1726, 1874, 2269, 2482
BstPAI	GACNNNNGTC	1 cut(s)	428
BstPI	GGTNACC	1 cut(s)	1637
BstSCI	CCNGG	9 cut(s)	177, 328, 1045, 1146, 1512, 1849, 2173, 2278, 2598
BstSFI	CTRYAG	5 cut(s)	378, 408, 1783, 1899, 2638
BstSLI	GKGCMC	2 cut(s)	1485, 1892
BstUI	CGCG	1 cut(s)	2142
BstV1I	GCAGC	8 cut(s)	21, 82, 256, 364, 394, 2270, 2605, 2608
BstV2I	GAAGAC	3 cut(s)	235, 1687, 2268
BstX2I	RGATCY	9 cut(s)	28, 235, 654, 1021, 1177, 1504, 1799, 2317, 2337
BstYI	RGATCY	9 cut(s)	28, 235, 654, 1021, 1177, 1504, 1799, 2317, 2337
BstZI	CGGCCG	1 cut(s)	180
Bsu36I	CCTNAGG	1 cut(s)	2196
BsuRI	GGCC	9 cut(s)	182, 201, 350, 585, 728, 1565, 2110, 2178, 2257
BtsCI	GGATG	9 cut(s)	112, 268, 394, 903, 1000, 1168, 1225, 1509, 1954
BtsI	GCAGTG	1 cut(s)	516
BtsIMutI	CAGTG	5 cut(s)	516, 914, 1440, 1908, 1982
Cac8I	GCNNGC	8 cut(s)	532, 790, 844, 1093, 1199, 1279, 1531, 1608
CciI	TCATGA	2 cut(s)	891, 1171
CfoI	GCGC	2 cut(s)	14, 112
Cfr13I	GGNCC	6 cut(s)	200, 325, 349, 1071, 1416, 2255
CseI	GACGC	2 cut(s)	38, 162
Csp6I	GTAC	4 cut(s)	795, 1381, 1682, 1819
CspCI	CAANNNNNGTGG	2 cut(s)	1041, 1076
CviQI	GTAC	4 cut(s)	795, 1381, 1682, 1819
DraI	TTTAAA	2 cut(s)	1078, 2355
EaeI	YGGCCR	5 cut(s)	180, 583, 726, 1563, 2108
EagI	CGGCCG	1 cut(s)	180
Eam1104I	CTCTTC	2 cut(s)	1236, 1596
EarI	CTCTTC	2 cut(s)	1236, 1596
Ecl136II	GAGCTC	1 cut(s)	2151
EclXI	CGGCCG	1 cut(s)	180
Eco130I	CCWWGG	2 cut(s)	1067, 1309
Eco24I	GRGCYC	3 cut(s)	1045, 1608, 2153
Eco31I	GGTCTC	2 cut(s)	23, 1360
Eco32I	GATATC	1 cut(s)	1807
Eco47I	GGWCC	3 cut(s)	325, 1071, 1416
Eco47III	AGCGCT	1 cut(s)	13
Eco52I	CGGCCG	1 cut(s)	180
Eco53kI	GAGCTC	1 cut(s)	2151
Eco57I	CTGAAG	3 cut(s)	45, 526, 750
Eco81I	CCTNAGG	1 cut(s)	2196
Eco88I	CYCGRG	1 cut(s)	2081
Eco91I	GGTNACC	1 cut(s)	1637
EcoICRI	GAGCTC	1 cut(s)	2151
EcoNI	CCTNNNNNAGG	1 cut(s)	1938
EcoO109I	RGGNCCY	2 cut(s)	349, 1071
EcoO65I	GGTNACC	1 cut(s)	1637
EcoRI	GAATTC	2 cut(s)	1761, 2028
EcoRII	CCWGG	6 cut(s)	1045, 1146, 1849, 2173, 2278, 2598
EcoRV	GATATC	1 cut(s)	1807
EcoT14I	CCWWGG	2 cut(s)	1067, 1309
EcoT22I	ATGCAT	2 cut(s)	1704, 1728
EcoT38I	GRGCYC	3 cut(s)	1045, 1608, 2153
ErhI	CCWWGG	2 cut(s)	1067, 1309
FaqI	GGGAC	5 cut(s)	160, 311, 321, 326, 587
FauI	CCCGC	2 cut(s)	1286, 1615
FauNDI	CATATG	1 cut(s)	2401
Fnu4HI	GCNGC	9 cut(s)	10, 71, 183, 270, 378, 408, 2284, 2619, 2622
FokI	GGATG	9 cut(s)	119, 275, 401, 890, 1007, 1175, 1232, 1496, 1961
FriOI	GRGCYC	3 cut(s)	1045, 1608, 2153
Fsp4HI	GCNGC	9 cut(s)	10, 71, 183, 270, 378, 408, 2284, 2619, 2622
FspBI	CTAG	2 cut(s)	960, 2393
FspI	TGCGCA	1 cut(s)	111
GlaI	GCGC	2 cut(s)	13, 111
GluI	GCNGC	9 cut(s)	10, 71, 183, 270, 378, 408, 2284, 2619, 2622
GsuI	CTGGAG	2 cut(s)	683, 1068
HaeII	RGCGCY	1 cut(s)	15
HaeIII	GGCC	9 cut(s)	182, 201, 350, 585, 728, 1565, 2110, 2178, 2257
HapII	CCGG	5 cut(s)	179, 253, 330, 582, 1513
HgaI	GACGC	2 cut(s)	38, 162
HhaI	GCGC	2 cut(s)	14, 112
Hin1I	GRCGYC	1 cut(s)	173
Hin6I	GCGC	2 cut(s)	12, 110
HinP1I	GCGC	2 cut(s)	12, 110
HincII	GTYRAC	4 cut(s)	858, 937, 2407, 2436
HindII	GTYRAC	4 cut(s)	858, 937, 2407, 2436
HindIII	AAGCTT	1 cut(s)	929
HpaII	CCGG	5 cut(s)	179, 253, 330, 582, 1513
HphI	GGTGA	4 cut(s)	302, 1235, 1649, 2471
Hpy166II	GTNNAC	7 cut(s)	79, 797, 858, 937, 1954, 2407, 2436
Hpy8I	GTNNAC	7 cut(s)	79, 797, 858, 937, 1954, 2407, 2436
Hpy99I	CGWCG	1 cut(s)	2458
HpyAV	CCTTC	4 cut(s)	529, 701, 1410, 2233
HpyCH4III	ACNGT	4 cut(s)	95, 1432, 1986, 2206
Hsp92I	GRCGYC	1 cut(s)	173
HspAI	GCGC	2 cut(s)	12, 110
Kpn2I	TCCGGA	1 cut(s)	252
LguI	GCTCTTC	1 cut(s)	1596
LmnI	GCTCC	8 cut(s)	905, 1048, 1049, 1069, 1105, 1714, 1900, 2245
Lsp1109I	GCAGC	8 cut(s)	21, 82, 256, 364, 394, 2270, 2605, 2608
LweI	GCATC	6 cut(s)	97, 256, 576, 1401, 1603, 1711
MaeI	CTAG	2 cut(s)	960, 2393
MaeIII	GTNAC	8 cut(s)	365, 561, 1360, 1386, 1637, 1906, 1980, 2474
MbiI	CCGCTC	1 cut(s)	1895
MflI	RGATCY	9 cut(s)	28, 235, 654, 1021, 1177, 1504, 1799, 2317, 2337
MhlI	GDGCHC	5 cut(s)	1045, 1485, 1608, 1892, 2153
MlsI	TGGCCA	2 cut(s)	728, 2110
MluNI	TGGCCA	2 cut(s)	728, 2110
MlyI	GAGTC	7 cut(s)	50, 56, 524, 560, 1280, 1773, 1988
MmeI	TCCRAC	4 cut(s)	84, 1140, 1968, 2295
Mox20I	TGGCCA	2 cut(s)	728, 2110
Mph1103I	ATGCAT	2 cut(s)	1704, 1728
MroI	TCCGGA	1 cut(s)	252
MroXI	GAANNNNTTC	1 cut(s)	1858
MscI	TGGCCA	2 cut(s)	728, 2110
MslI	CAYNNNNRTG	2 cut(s)	1478, 2091
Msp20I	TGGCCA	2 cut(s)	728, 2110
MspA1I	CMGCKG	2 cut(s)	1610, 1645
MspI	CCGG	5 cut(s)	179, 253, 330, 582, 1513
MspR9I	CCNGG	9 cut(s)	179, 330, 1047, 1148, 1514, 1851, 2175, 2280, 2600
Mva1269I	GAATGC	1 cut(s)	2231
MvaI	CCWGG	6 cut(s)	1047, 1148, 1851, 2175, 2280, 2600
MvnI	CGCG	1 cut(s)	2142
NciI	CCSGG	3 cut(s)	179, 330, 1514
NdeI	CATATG	1 cut(s)	2401
NlaIV	GGNNCC	6 cut(s)	327, 829, 1044, 1065, 1891, 2247
NmeAIII	GCCGAG	1 cut(s)	1591
NmuCI	GTSAC	5 cut(s)	365, 1386, 1637, 1906, 1980
NsbI	TGCGCA	1 cut(s)	111
NsiI	ATGCAT	2 cut(s)	1704, 1728
NspI	RCATGY	7 cut(s)	534, 562, 1533, 1726, 1874, 2269, 2482
NspV	TTCGAA	1 cut(s)	1454
PaeI	GCATGC	2 cut(s)	534, 1533
PagI	TCATGA	2 cut(s)	891, 1171
PciI	ACATGT	3 cut(s)	558, 1870, 2265
PciSI	GCTCTTC	1 cut(s)	1596
PctI	GAATGC	1 cut(s)	2231
PdmI	GAANNNNTTC	1 cut(s)	1858
PfeI	GAWTC	9 cut(s)	38, 116, 131, 137, 689, 1130, 1859, 1913, 2376
PfoI	TCCNGGA	1 cut(s)	1045
PkrI	GCNGC	9 cut(s)	11, 72, 184, 271, 379, 409, 2285, 2620, 2623
Ple19I	CGATCG	1 cut(s)	2557
PleI	GAGTC	7 cut(s)	50, 56, 523, 559, 1280, 1773, 1987
PpsI	GAGTC	7 cut(s)	50, 56, 523, 559, 1280, 1773, 1987
PpuMI	RGGWCCY	1 cut(s)	1071
PscI	ACATGT	3 cut(s)	558, 1870, 2265
PshAI	GACNNNNGTC	1 cut(s)	428
PshBI	ATTAAT	1 cut(s)	1476
Psp124BI	GAGCTC	1 cut(s)	2153
Psp5II	RGGWCCY	1 cut(s)	1071
Psp6I	CCWGG	6 cut(s)	1045, 1146, 1849, 2173, 2278, 2598
PspEI	GGTNACC	1 cut(s)	1637
PspGI	CCWGG	6 cut(s)	1045, 1146, 1849, 2173, 2278, 2598
PspN4I	GGNNCC	6 cut(s)	327, 829, 1044, 1065, 1891, 2247
PspPI	GGNCC	6 cut(s)	200, 325, 349, 1071, 1416, 2255
PspPPI	RGGWCCY	1 cut(s)	1071
PstI	CTGCAG	2 cut(s)	382, 412
PsuI	RGATCY	9 cut(s)	28, 235, 654, 1021, 1177, 1504, 1799, 2317, 2337
PvuI	CGATCG	1 cut(s)	2557
PvuII	CAGCTG	1 cut(s)	1645
RsaI	GTAC	4 cut(s)	796, 1382, 1683, 1820
RsaNI	GTAC	4 cut(s)	795, 1381, 1682, 1819
RseI	CAYNNNNRTG	2 cut(s)	1478, 2091
SacI	GAGCTC	1 cut(s)	2153
SapI	GCTCTTC	1 cut(s)	1596
SatI	GCNGC	9 cut(s)	10, 71, 183, 270, 378, 408, 2284, 2619, 2622
Sau96I	GGNCC	6 cut(s)	200, 325, 349, 1071, 1416, 2255
ScaI	AGTACT	1 cut(s)	1820
SchI	GAGTC	7 cut(s)	50, 56, 524, 560, 1280, 1773, 1988
ScrFI	CCNGG	9 cut(s)	179, 330, 1047, 1148, 1514, 1851, 2175, 2280, 2600
SduI	GDGCHC	5 cut(s)	1045, 1485, 1608, 1892, 2153
SfaNI	GCATC	6 cut(s)	97, 256, 576, 1401, 1603, 1711
SfcI	CTRYAG	5 cut(s)	378, 408, 1783, 1899, 2638
SfuI	TTCGAA	1 cut(s)	1454
SinI	GGWCC	3 cut(s)	325, 1071, 1416
SmiMI	CAYNNNNRTG	2 cut(s)	1478, 2091
SphI	GCATGC	2 cut(s)	534, 1533
SsiI	CCGC	8 cut(s)	183, 1279, 1414, 1608, 1893, 1926, 2359, 2520
SspI	AATATT	1 cut(s)	1259
SspMI	CTAG	2 cut(s)	960, 2393
SstI	GAGCTC	1 cut(s)	2153
StyD4I	CCNGG	9 cut(s)	177, 328, 1045, 1146, 1512, 1849, 2173, 2278, 2598
StyI	CCWWGG	2 cut(s)	1067, 1309
TaaI	ACNGT	4 cut(s)	95, 1432, 1986, 2206
TaqI	TCGA	5 cut(s)	1231, 1403, 1454, 2124, 2374
TatI	WGTACW	2 cut(s)	794, 1818
TauI	GCSGC	1 cut(s)	185
TfiI	GAWTC	9 cut(s)	38, 116, 131, 137, 689, 1130, 1859, 1913, 2376
TscAI	CASTG	5 cut(s)	516, 914, 1447, 1915, 1989
TseFI	GTSAC	5 cut(s)	365, 1386, 1637, 1906, 1980
TseI	GCWGC	8 cut(s)	9, 70, 269, 377, 407, 2283, 2618, 2621
Tsp45I	GTSAC	5 cut(s)	365, 1386, 1637, 1906, 1980
TspGWI	ACGGA	1 cut(s)	1362
TspRI	CASTG	5 cut(s)	516, 914, 1447, 1915, 1989
VpaK11BI	GGWCC	3 cut(s)	325, 1071, 1416
VspI	ATTAAT	1 cut(s)	1476
XagI	CCTNNNNNAGG	1 cut(s)	1938
XapI	RAATTY	6 cut(s)	472, 751, 1449, 1761, 1997, 2028
XceI	RCATGY	7 cut(s)	534, 562, 1533, 1726, 1874, 2269, 2482
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2265
XmnI	GAANNNNTTC	1 cut(s)	1858
XspI	CTAG	2 cut(s)	960, 2393
ZrmI	AGTACT	1 cut(s)	1820
Zsp2I	ATGCAT	2 cut(s)	1704, 1728

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.