Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5C

Orthogroup ID

OG0004829

Physical Location & Seq

Genomic Coordinates Forward (+)

42250841 .. 42261039

Gene Structure 10199 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5CG358200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2655 bp

ATGGAGAGCGCTGGTCTCTCTGAAGATCTCTCCGATTCCACTCAGCGTCCCGACTCCGACTCCGACAGCGAGCAGCGAGTTTACTTTGTTCCCTACAGTTGGTGGAGGGATGCACAAGATTCAGTTGTGGGCGATTCAGATGGGAACAAAGGGGTTTTGTGTGTGGCATCCCCGGCCGCTTCGTCTTATGGTGGCCCTATGAAGATCATCAACAACATTTTCAACTCAGATCTCGTCTTCAATCTCAGGAGAGAGGATGATGCTGTGCACAATGCTGAAAATGCCGAGGTCGGTGTATCGGGCCGGGACTATGCATTGGTCCCCGGAGAAATGTGGGTTCAGGCGCTCAGATGGCATAGTGATCCTAAAGCTGCAGTTAAGGATGTTAGGAGTTTTTCGGCTGCAGAAGATGACAAGACCGATGTCTATCCCCTACAACTGAGGCTTTCTGTTCCACGAGAAACAAATTCATTGGGCGTAAGAATTACCAAAAAGGATAATGCAGTTGAGTCCTTCAAAAGAGCCTGCAAAATCTTCAGTTTGGAGTCTGACTTGTTACGCATCTGGGACTTTTCCGGCCAGACTAACTTATTTTTTACAAATGACAAAACCAAGTTTCCAAGAGACTTGCAACGGCAGTCAGATGAGATCCTACTGGAGTTGCAAGTTTATGGATTGTCAGATTCCATCAAGTGCAGGGAAGGGAAAAAAGATGAGATGGCCATGCAACATTCTACTATTGGAAGTTCTTGTTCTGGTTCTTCAGTTATGATGAATGGTTCTGCTGGCAATATGCATTCCAATTTCATTCGTTCTTTCTGGGGAAGCTCTTGTGAAGCTGGTTCTTTAGGGTTGACAGGATTGCAAAACCTCGGAAATACTTGTTTCATGAATAGCTCCATCCAGTGCTTAGCTCATACACCAAAGCTTGTTGACTATTTTCTTGGAGATTATGCTAGAGAAATAAATCAGGAAAACCCATTGGGCATGGATGGGGAGATTGCTTTAGCATTTGGAGATCTGTTAAGGAAGTTATGGGCTCCTGGAGCAACTCCTGTGCCTCCAAGGACCTTTAAATCAAAACTTGCTCGTTTTGCTCCTCAGTTTAATGGCTTTAATCAACATGATTCACAGGAACTCCTGGCCTTTTTGTTGGATGGACTTCATGAAGATCTTAATCGTGTAAAATGCAAGCCTTACGTAGAAGTCAAGGATGGTGATGATCGACCGGATGAAGAGGTAGCTGATGAATATTGGCGAAATCATTTAGCCCGCAATGACTCTATCATTGTTGATGTGTGCCAAGGTCAATATAAATCAACATTAGTTTGTCCTGTTTGCAAAAAGGTCTCTGTTACATTTGATCCGTTTATGTACCTGTCACTACCATTACCTTCAACAATGATGCGGACAATGACTTTAACAGTTATCAACACTGATGGAAGTTCGAAGCCATCTGAATATACCATTAATGTGCCCAAGCATGGAAAGTTTGACGATCTCATCCGCAGTTTGAGTGTTGCATGCTCACTTGGGGTTGATGAAACCTTTTTAGTGGCCGAGATATACAACAATCGCATTATACGTTATCTCGAGGAGCCTGCTGATTCATTATCCTTAATTAGAGATGGTGACCAGTTGGTTGCTTATCGGTTTATGAAAGATGTTGAGGAAGTACCTCTGGTAGTTTTCATGCATCAACAAATGGAGCAGGACATGCATGGGAAGTTGAGTTCAAGCTGGAAACCGTTTGGAATTCCTCTTGTATCAAGACTTCGTACTTCGGCAACTGGATCTGATATCTGCTATCAGTACTTAAACATAGTTAAGCCATTTCAAATACCTGGAAAAGATTCCCAAGCACTTGTTGATAATTCACAGGGCACTGCTCCTATAGAGGCCACTGGAGCAGAGGGTACCGCACCTCCTGTTTCAGGTGGGGATGTGAACCTCTGTTATGAAAATGGGGCTGACTCACCATTGGATTGTGAATTTCAGTTTTATATAACAGATGAAAAGGGTATTCAAAAGGAGAAAAAGATAGTAATGAATGAGATAGTCGAGAGGAAAATGTCGGAGCATTTATATGTTGCAGTATGTTGGCCAGAAAAATACGTTGAGCAGTATGATACTCGCGTTCTGAGCTCATTGCCATTGGTTTTCAAGCCTGGCCTTTTTTCTAAAAAGCCTCAGGAGACTGTTTCTCTATATAAGTGCCTTGAAGCATTCTTGAAGGAGGAGCCTCTTGGGCCAGAAGACATGTGGTATTGTCCTGGCTGCAAAAAGCATTGCCAGGCCAGTAAGAAGTTGGATCTCTGGAGACTGCCTGAGATCTTGGTCATTCATTTAAAGCGGTTCTCATATAGTCGATTCTTCAAGAACAAGCTAGAGACATATGTTGACTTCCCTGTTGATAATCTCGATTTGTCAACATACATTGCTTACAGGACTGGTGAATTAAGCAATCGTTACATGCTTTATGCAGTTAGTAATCACTATGGAAGCATGGGTGGCGGTCATTATACTGCATTTGTCCATCATGGGGGCGATCGGTGGTATGACTTTGATGATAGCCATGTTAGCCCTGTGAGCCAGGACAAGATAAAGTCTGCTGCTGCGTATGTTCTTTTCTATAGGAGAGTAGTAGAAGTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko04137 map04137

Pfam Domains

Protein Families

UCH

Protein Analysis

884

Amino Acids

99.53

Weight (kDa)

5.3

Isoelectric Point (pI)

43.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ubiquitin_UBP8	PF25242	147 - 201	4.5e-30	UBP8-like, ubiquitin-like domain
UCH	PF00443	286 - 878	1.3e-74	Ubiquitin carboxyl-terminal hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0004829)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G22030 AT5G22030
fragaria_vesca	FvH4_3g26140 FvH4_3g26140 FvH4_3g26140 FvH4_3g26140 FvH4_3g26140 FvH4_3g26140 FvH4_3g26140
malus_domestica	MD03G1159600.v1.1 MD11G1174900.v1.1
prunus_persica	Prupe.6G147300_v2.0.a1 Prupe.6G147300_v2.0.a1 Prupe.6G147300_v2.0.a1 Prupe.6G147300_v2.0.a1
pyrus_communis	pycom03g11220 pycom03g11230 pycom11g14960 pycom11g14970
rosa_chinensis	RchiOBHm_Chr5g0049121
rosa_laevigata	RLG00000034590
rosa_multiflora	Rmu_sc0000530.1_g000038 Rmu_sc0000530.1_g000039
rosa_roxburghii	Rroxscaffold_1G00032700
rosa_rugosa	Rorug05G0243300 Rorug05G0243300
rosa_samantha	Rh5AG322700 Rh5BG333300 Rh5CG358200 Rh5DG344700
rosa_wichuraiana	Rw5G030460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	1916
AccB1I	GGYRCC	1 cut(s)	1916
AccII	CGCG	1 cut(s)	2136
AciI	CCGC	7 cut(s)	177, 1273, 1408, 1507, 1920, 2353, 2514
AclWI	GGATC	5 cut(s)	356, 643, 1358, 1801, 2319
AcoI	YGGCCR	5 cut(s)	174, 577, 720, 1557, 2102
AcsI	RAATTY	3 cut(s)	466, 1755, 1991
AcuI	CTGAAG	3 cut(s)	42, 520, 747
AfaI	GTAC	5 cut(s)	1376, 1677, 1780, 1814, 1918
AfeI	AGCGCT	1 cut(s)	10
AfiI	CCNNNNNNNGG	4 cut(s)	99, 1484, 1934, 2542
AflIII	ACRYGT	1 cut(s)	2259
AjnI	CCWGG	7 cut(s)	1042, 1140, 1843, 2167, 2272, 2292, 2592
AloI	GAACNNNNNNTCC	2 cut(s)	1717, 1749
Alw21I	GWGCWC	2 cut(s)	270, 2147
Alw26I	GTCTC	6 cut(s)	20, 618, 1354, 2189, 2314, 2384
Alw44I	GTGCAC	1 cut(s)	266
AlwI	GGATC	5 cut(s)	356, 643, 1358, 1801, 2319
Ama87I	CYCGRG	1 cut(s)	1592
Aor51HI	AGCGCT	1 cut(s)	10
ApaLI	GTGCAC	1 cut(s)	266
ApeKI	GCWGC	6 cut(s)	73, 371, 401, 2277, 2612, 2615
ApoI	RAATTY	3 cut(s)	466, 1755, 1991
AseI	ATTAAT	1 cut(s)	1470
Asp700I	GAANNNNTTC	1 cut(s)	1852
Asp718I	GGTACC	1 cut(s)	1916
AspLEI	GCGC	2 cut(s)	11, 346
AspS9I	GGNCC	5 cut(s)	194, 301, 319, 1068, 2249
AsuC2I	CCSGG	3 cut(s)	173, 305, 324
AsuHPI	GGTGA	4 cut(s)	1229, 1643, 1968, 2465
AsuII	TTCGAA	1 cut(s)	1448
AvaI	CYCGRG	1 cut(s)	1592
AvaII	GGWCC	2 cut(s)	319, 1068
AxyI	CCTNAGG	1 cut(s)	2190
BaeGI	GKGCMC	3 cut(s)	270, 1479, 1886
BaeI	ACNNNNGTAYC	2 cut(s)	2121, 2154
BalI	TGGCCA	2 cut(s)	722, 2104
BanI	GGYRCC	1 cut(s)	1916
BanII	GRGCYC	2 cut(s)	1042, 2147
BarI	GAAGNNNNNNTAC	2 cut(s)	1758, 1790
BauI	CACGAG	1 cut(s)	456
BbsI	GAAGAC	2 cut(s)	229, 2262
Bbv12I	GWGCWC	2 cut(s)	270, 2147
BbvI	GCAGC	6 cut(s)	85, 358, 388, 2264, 2599, 2602
BceAI	ACGGC	1 cut(s)	650
BcgI	CGANNNNNNTGC	2 cut(s)	1583, 1617
BciT130I	CCWGG	7 cut(s)	1044, 1142, 1845, 2169, 2274, 2294, 2594
BcnI	CCSGG	3 cut(s)	173, 305, 324
BcoDI	GTCTC	6 cut(s)	20, 618, 1354, 2189, 2314, 2384
BfaI	CTAG	2 cut(s)	957, 2387
BfmI	CTRYAG	5 cut(s)	94, 372, 402, 1893, 2632
BfoI	RGCGCY	2 cut(s)	12, 347
BglI	GCCNNNNNGGC	1 cut(s)	2248
BglII	AGATCT	5 cut(s)	25, 229, 1018, 1171, 2331
BisI	GCNGC	7 cut(s)	74, 177, 372, 402, 2278, 2613, 2616
BlpI	GCTNAGC	1 cut(s)	910
BlsI	GCNGC	7 cut(s)	75, 178, 373, 403, 2279, 2614, 2617
BmcAI	AGTACT	1 cut(s)	1814
Bme18I	GGWCC	2 cut(s)	319, 1068
BmeT110I	CYCGRG	1 cut(s)	1592
BmgT120I	GGNCC	5 cut(s)	194, 301, 319, 1068, 2249
BmiI	GGNNCC	5 cut(s)	321, 1041, 1599, 1918, 2241
BmsI	GCATC	6 cut(s)	100, 176, 250, 570, 1395, 1705
BoxI	GACNNNNGTC	1 cut(s)	422
BpiI	GAAGAC	2 cut(s)	229, 2262
BpmI	CTGGAG	4 cut(s)	677, 1065, 1926, 2338
Bpu1102I	GCTNAGC	1 cut(s)	910
Bpu14I	TTCGAA	1 cut(s)	1448
BpuMI	CCSGG	3 cut(s)	173, 305, 324
BsaAI	YACGTR	1 cut(s)	1201
BsaBI	GATNNNNATC	2 cut(s)	426, 1176
BsaI	GGTCTC	2 cut(s)	20, 1354
BsaJI	CCNNGG	6 cut(s)	171, 285, 322, 871, 1064, 1303
BsaWI	WCCGGW	1 cut(s)	1228
BsaXI	ACNNNNNCTCC	6 cut(s)	44, 74, 1122, 1152, 1909, 1939
Bsc4I	CCNNNNNNNGG	4 cut(s)	99, 1484, 1934, 2542
Bse1I	ACTGG	7 cut(s)	660, 904, 1636, 1795, 1909, 2298, 2455
Bse21I	CCTNAGG	1 cut(s)	2190
Bse3DI	GCAATG	4 cut(s)	1282, 2147, 2287, 2436
Bse8I	GATNNNNATC	2 cut(s)	426, 1176
BseBI	CCWGG	7 cut(s)	1044, 1142, 1845, 2169, 2274, 2294, 2594
BseDI	CCNNGG	6 cut(s)	171, 285, 322, 871, 1064, 1303
BseJI	GATNNNNATC	2 cut(s)	426, 1176
BseLI	CCNNNNNNNGG	4 cut(s)	99, 1484, 1934, 2542
BseMI	GCAATG	4 cut(s)	1282, 2147, 2287, 2436
BseMII	CTCAG	9 cut(s)	56, 240, 259, 361, 431, 1115, 2132, 2204, 2319
BseNI	ACTGG	7 cut(s)	660, 904, 1636, 1795, 1909, 2298, 2455
BseRI	GAGGAG	3 cut(s)	1089, 1610, 2252
BseSI	GKGCMC	3 cut(s)	270, 1479, 1886
BseX3I	CGGCCG	1 cut(s)	174
BseXI	GCAGC	6 cut(s)	85, 358, 388, 2264, 2599, 2602
BsgI	GTGCAG	1 cut(s)	715
Bsh1236I	CGCG	1 cut(s)	2136
Bsh1285I	CGRYCG	3 cut(s)	177, 1229, 2551
BshNI	GGYRCC	1 cut(s)	1916
BsiEI	CGRYCG	3 cut(s)	177, 1229, 2551
BsiHKAI	GWGCWC	2 cut(s)	270, 2147
BsiHKCI	CYCGRG	1 cut(s)	1592
BsiSI	CCGG	5 cut(s)	173, 304, 324, 576, 1229
BslFI	GGGAC	4 cut(s)	33, 305, 320, 581
BslI	CCNNNNNNNGG	4 cut(s)	99, 1484, 1934, 2542
BsmAI	GTCTC	6 cut(s)	20, 618, 1354, 2189, 2314, 2384
BsmFI	GGGAC	4 cut(s)	33, 305, 320, 581
BsmI	GAATGC	2 cut(s)	796, 2225
Bso31I	GGTCTC	2 cut(s)	20, 1354
BsoBI	CYCGRG	1 cut(s)	1592
Bsp119I	TTCGAA	1 cut(s)	1448
Bsp1286I	GDGCHC	5 cut(s)	270, 1042, 1479, 1886, 2147
Bsp1720I	GCTNAGC	1 cut(s)	910
BspACI	CCGC	7 cut(s)	177, 1273, 1408, 1507, 1920, 2353, 2514
BspCNI	CTCAG	9 cut(s)	55, 239, 258, 360, 432, 1114, 2133, 2203, 2320
BspFNI	CGCG	1 cut(s)	2136
BspHI	TCATGA	2 cut(s)	888, 1165
BspLI	GGNNCC	5 cut(s)	321, 1041, 1599, 1918, 2241
BspMAI	CTGCAG	2 cut(s)	376, 406
BspPI	GGATC	5 cut(s)	356, 643, 1358, 1801, 2319
BspT104I	TTCGAA	1 cut(s)	1448
BspT107I	GGYRCC	1 cut(s)	1916
BspTNI	GGTCTC	2 cut(s)	20, 1354
BsrDI	GCAATG	4 cut(s)	1282, 2147, 2287, 2436
BsrI	ACTGG	7 cut(s)	660, 904, 1636, 1795, 1909, 2298, 2455
BssECI	CCNNGG	6 cut(s)	171, 285, 322, 871, 1064, 1303
BssSI	CACGAG	1 cut(s)	456
BssT1I	CCWWGG	2 cut(s)	1064, 1303
Bst2BI	CACGAG	1 cut(s)	456
Bst2UI	CCWGG	7 cut(s)	1044, 1142, 1845, 2169, 2274, 2294, 2594
Bst4CI	ACNGT	4 cut(s)	98, 1426, 1749, 2200
Bst6I	CTCTTC	1 cut(s)	1230
BstAPI	GCANNNNNTGC	1 cut(s)	1717
BstBAI	YACGTR	1 cut(s)	1201
BstBI	TTCGAA	1 cut(s)	1448
BstC8I	GCNNGC	7 cut(s)	71, 526, 787, 1193, 1273, 1525, 1602
BstEII	GGTNACC	1 cut(s)	1631
BstENI	CCTNNNNNAGG	1 cut(s)	1932
BstFNI	CGCG	1 cut(s)	2136
BstH2I	RGCGCY	2 cut(s)	12, 347
BstHHI	GCGC	2 cut(s)	11, 346
BstMAI	GTCTC	6 cut(s)	20, 618, 1354, 2189, 2314, 2384
BstMCI	CGRYCG	3 cut(s)	177, 1229, 2551
BstNI	CCWGG	7 cut(s)	1044, 1142, 1845, 2169, 2274, 2294, 2594
BstNSI	RCATGY	4 cut(s)	1527, 1720, 2263, 2476
BstPAI	GACNNNNGTC	1 cut(s)	422
BstPI	GGTNACC	1 cut(s)	1631
BstSFI	CTRYAG	5 cut(s)	94, 372, 402, 1893, 2632
BstSLI	GKGCMC	3 cut(s)	270, 1479, 1886
BstSNI	TACGTA	1 cut(s)	1201
BstUI	CGCG	1 cut(s)	2136
BstV1I	GCAGC	6 cut(s)	85, 358, 388, 2264, 2599, 2602
BstV2I	GAAGAC	2 cut(s)	229, 2262
BstX2I	RGATCY	8 cut(s)	25, 229, 648, 1018, 1171, 1793, 2311, 2331
BstYI	RGATCY	8 cut(s)	25, 229, 648, 1018, 1171, 1793, 2311, 2331
BstZI	CGGCCG	1 cut(s)	174
Bsu36I	CCTNAGG	1 cut(s)	2190
BtsI	GCAGTG	1 cut(s)	1884
BtsIMutI	CAGTG	4 cut(s)	911, 1434, 1884, 1902
Cac8I	GCNNGC	7 cut(s)	71, 526, 787, 1193, 1273, 1525, 1602
CciI	TCATGA	2 cut(s)	888, 1165
CfoI	GCGC	2 cut(s)	11, 346
Cfr13I	GGNCC	5 cut(s)	194, 301, 319, 1068, 2249
CseI	GACGC	1 cut(s)	35
Csp6I	GTAC	5 cut(s)	1375, 1676, 1779, 1813, 1917
CviQI	GTAC	5 cut(s)	1375, 1676, 1779, 1813, 1917
DraI	TTTAAA	2 cut(s)	1075, 2349
EaeI	YGGCCR	5 cut(s)	174, 577, 720, 1557, 2102
EagI	CGGCCG	1 cut(s)	174
Eam1104I	CTCTTC	1 cut(s)	1230
EarI	CTCTTC	1 cut(s)	1230
Ecl136II	GAGCTC	1 cut(s)	2145
EclXI	CGGCCG	1 cut(s)	174
Eco105I	TACGTA	1 cut(s)	1201
Eco130I	CCWWGG	2 cut(s)	1064, 1303
Eco24I	GRGCYC	2 cut(s)	1042, 2147
Eco31I	GGTCTC	2 cut(s)	20, 1354
Eco32I	GATATC	1 cut(s)	1801
Eco47I	GGWCC	2 cut(s)	319, 1068
Eco47III	AGCGCT	1 cut(s)	10
Eco52I	CGGCCG	1 cut(s)	174
Eco53kI	GAGCTC	1 cut(s)	2145
Eco57I	CTGAAG	3 cut(s)	42, 520, 747
Eco81I	CCTNAGG	1 cut(s)	2190
Eco88I	CYCGRG	1 cut(s)	1592
Eco91I	GGTNACC	1 cut(s)	1631
EcoICRI	GAGCTC	1 cut(s)	2145
EcoNI	CCTNNNNNAGG	1 cut(s)	1932
EcoO109I	RGGNCCY	1 cut(s)	1068
EcoO65I	GGTNACC	1 cut(s)	1631
EcoRI	GAATTC	1 cut(s)	1755
EcoRII	CCWGG	7 cut(s)	1042, 1140, 1843, 2167, 2272, 2292, 2592
EcoRV	GATATC	1 cut(s)	1801
EcoT14I	CCWWGG	2 cut(s)	1064, 1303
EcoT22I	ATGCAT	4 cut(s)	316, 798, 1698, 1722
EcoT38I	GRGCYC	2 cut(s)	1042, 2147
ErhI	CCWWGG	2 cut(s)	1064, 1303
FaqI	GGGAC	4 cut(s)	33, 305, 320, 581
FauI	CCCGC	1 cut(s)	1280
FauNDI	CATATG	1 cut(s)	2395
Fnu4HI	GCNGC	7 cut(s)	74, 177, 372, 402, 2278, 2613, 2616
FriOI	GRGCYC	2 cut(s)	1042, 2147
Fsp4HI	GCNGC	7 cut(s)	74, 177, 372, 402, 2278, 2613, 2616
FspBI	CTAG	2 cut(s)	957, 2387
GlaI	GCGC	2 cut(s)	10, 345
GluI	GCNGC	7 cut(s)	74, 177, 372, 402, 2278, 2613, 2616
GsuI	CTGGAG	4 cut(s)	677, 1065, 1926, 2338
HaeII	RGCGCY	2 cut(s)	12, 347
HapII	CCGG	5 cut(s)	173, 304, 324, 576, 1229
HgaI	GACGC	1 cut(s)	35
HhaI	GCGC	2 cut(s)	11, 346
Hin6I	GCGC	2 cut(s)	9, 344
HinP1I	GCGC	2 cut(s)	9, 344
HincII	GTYRAC	4 cut(s)	855, 934, 2401, 2430
HindII	GTYRAC	4 cut(s)	855, 934, 2401, 2430
HindIII	AAGCTT	1 cut(s)	926
HpaII	CCGG	5 cut(s)	173, 304, 324, 576, 1229
HphI	GGTGA	4 cut(s)	1229, 1643, 1968, 2465
Hpy166II	GTNNAC	7 cut(s)	82, 268, 855, 934, 1948, 2401, 2430
Hpy8I	GTNNAC	7 cut(s)	82, 268, 855, 934, 1948, 2401, 2430
HpyAV	CCTTC	4 cut(s)	523, 695, 1404, 2227
HpyCH4III	ACNGT	4 cut(s)	98, 1426, 1749, 2200
HpyCH4IV	ACGT	3 cut(s)	1200, 1585, 2115
HpySE526I	ACGT	3 cut(s)	1200, 1585, 2115
HspAI	GCGC	2 cut(s)	9, 344
KpnI	GGTACC	1 cut(s)	1920
Lsp1109I	GCAGC	6 cut(s)	85, 358, 388, 2264, 2599, 2602
LweI	GCATC	6 cut(s)	100, 176, 250, 570, 1395, 1705
MaeI	CTAG	2 cut(s)	957, 2387
MaeII	ACGT	3 cut(s)	1200, 1585, 2115
MaeIII	GTNAC	5 cut(s)	555, 1354, 1380, 1631, 2468
MflI	RGATCY	8 cut(s)	25, 229, 648, 1018, 1171, 1793, 2311, 2331
MhlI	GDGCHC	5 cut(s)	270, 1042, 1479, 1886, 2147
MlsI	TGGCCA	2 cut(s)	722, 2104
MluCI	AATT	8 cut(s)	466, 483, 802, 1620, 1755, 1873, 1991, 2456
MluNI	TGGCCA	2 cut(s)	722, 2104
MlyI	GAGTC	6 cut(s)	47, 53, 518, 554, 1274, 1967
MmeI	TCCRAC	5 cut(s)	81, 87, 1134, 2055, 2289
Mox20I	TGGCCA	2 cut(s)	722, 2104
Mph1103I	ATGCAT	4 cut(s)	316, 798, 1698, 1722
MroXI	GAANNNNTTC	1 cut(s)	1852
MscI	TGGCCA	2 cut(s)	722, 2104
MslI	CAYNNNNRTG	2 cut(s)	1472, 2085
Msp20I	TGGCCA	2 cut(s)	722, 2104
MspI	CCGG	5 cut(s)	173, 304, 324, 576, 1229
Mva1269I	GAATGC	2 cut(s)	796, 2225
MvaI	CCWGG	7 cut(s)	1044, 1142, 1845, 2169, 2274, 2294, 2594
MvnI	CGCG	1 cut(s)	2136
NciI	CCSGG	3 cut(s)	173, 305, 324
NdeI	CATATG	1 cut(s)	2395
NlaIV	GGNNCC	5 cut(s)	321, 1041, 1599, 1918, 2241
NmeAIII	GCCGAG	2 cut(s)	310, 1585
NmuCI	GTSAC	2 cut(s)	1380, 1631
NsiI	ATGCAT	4 cut(s)	316, 798, 1698, 1722
NspI	RCATGY	4 cut(s)	1527, 1720, 2263, 2476
NspV	TTCGAA	1 cut(s)	1448
PaeI	GCATGC	1 cut(s)	1527
PaeR7I	CTCGAG	1 cut(s)	1592
PagI	TCATGA	2 cut(s)	888, 1165
PciI	ACATGT	1 cut(s)	2259
PcsI	WCGNNNNNNNCGW	1 cut(s)	1582
PctI	GAATGC	2 cut(s)	796, 2225
PdmI	GAANNNNTTC	1 cut(s)	1852
PfeI	GAWTC	8 cut(s)	35, 119, 134, 683, 1127, 1607, 1853, 2370
PfoI	TCCNGGA	1 cut(s)	1042
PkrI	GCNGC	7 cut(s)	75, 178, 373, 403, 2279, 2614, 2617
Ple19I	CGATCG	1 cut(s)	2551
PleI	GAGTC	6 cut(s)	47, 53, 517, 553, 1274, 1967
PpsI	GAGTC	6 cut(s)	47, 53, 517, 553, 1274, 1967
Ppu21I	YACGTR	1 cut(s)	1201
PpuMI	RGGWCCY	1 cut(s)	1068
PscI	ACATGT	1 cut(s)	2259
PshAI	GACNNNNGTC	1 cut(s)	422
PshBI	ATTAAT	1 cut(s)	1470
Psp124BI	GAGCTC	1 cut(s)	2147
Psp5II	RGGWCCY	1 cut(s)	1068
Psp6I	CCWGG	7 cut(s)	1042, 1140, 1843, 2167, 2272, 2292, 2592
PspEI	GGTNACC	1 cut(s)	1631
PspGI	CCWGG	7 cut(s)	1042, 1140, 1843, 2167, 2272, 2292, 2592
PspN4I	GGNNCC	5 cut(s)	321, 1041, 1599, 1918, 2241
PspPI	GGNCC	5 cut(s)	194, 301, 319, 1068, 2249
PspPPI	RGGWCCY	1 cut(s)	1068
PstI	CTGCAG	2 cut(s)	376, 406
PsuI	RGATCY	8 cut(s)	25, 229, 648, 1018, 1171, 1793, 2311, 2331
PvuI	CGATCG	1 cut(s)	2551
RsaI	GTAC	5 cut(s)	1376, 1677, 1780, 1814, 1918
RsaNI	GTAC	5 cut(s)	1375, 1676, 1779, 1813, 1917
RseI	CAYNNNNRTG	2 cut(s)	1472, 2085
SacI	GAGCTC	1 cut(s)	2147
SatI	GCNGC	7 cut(s)	74, 177, 372, 402, 2278, 2613, 2616
Sau96I	GGNCC	5 cut(s)	194, 301, 319, 1068, 2249
ScaI	AGTACT	1 cut(s)	1814
SchI	GAGTC	6 cut(s)	47, 53, 518, 554, 1274, 1967
SduI	GDGCHC	5 cut(s)	270, 1042, 1479, 1886, 2147
SfaNI	GCATC	6 cut(s)	100, 176, 250, 570, 1395, 1705
SfcI	CTRYAG	5 cut(s)	94, 372, 402, 1893, 2632
Sfr274I	CTCGAG	1 cut(s)	1592
SfuI	TTCGAA	1 cut(s)	1448
SinI	GGWCC	2 cut(s)	319, 1068
SlaI	CTCGAG	1 cut(s)	1592
SmiMI	CAYNNNNRTG	2 cut(s)	1472, 2085
SmlI	CTYRAG	1 cut(s)	1592
SmoI	CTYRAG	1 cut(s)	1592
SnaBI	TACGTA	1 cut(s)	1201
SphI	GCATGC	1 cut(s)	1527
Sse9I	AATT	8 cut(s)	466, 483, 802, 1620, 1755, 1873, 1991, 2456
SsiI	CCGC	7 cut(s)	177, 1273, 1408, 1507, 1920, 2353, 2514
SspI	AATATT	1 cut(s)	1253
SspMI	CTAG	2 cut(s)	957, 2387
SstI	GAGCTC	1 cut(s)	2147
StyI	CCWWGG	2 cut(s)	1064, 1303
TaaI	ACNGT	4 cut(s)	98, 1426, 1749, 2200
TaiI	ACGT	3 cut(s)	1203, 1588, 2118
TaqI	TCGA	6 cut(s)	1225, 1448, 1593, 2061, 2368, 2421
TaqII	GACCGA	1 cut(s)	434
TasI	AATT	8 cut(s)	466, 483, 802, 1620, 1755, 1873, 1991, 2456
TatI	WGTACW	1 cut(s)	1812
TauI	GCSGC	1 cut(s)	179
TfiI	GAWTC	8 cut(s)	35, 119, 134, 683, 1127, 1607, 1853, 2370
TscAI	CASTG	4 cut(s)	911, 1441, 1891, 1909
TseFI	GTSAC	2 cut(s)	1380, 1631
TseI	GCWGC	6 cut(s)	73, 371, 401, 2277, 2612, 2615
Tsp45I	GTSAC	2 cut(s)	1380, 1631
TspGWI	ACGGA	1 cut(s)	1356
TspRI	CASTG	4 cut(s)	911, 1441, 1891, 1909
VneI	GTGCAC	1 cut(s)	266
VpaK11BI	GGWCC	2 cut(s)	319, 1068
VspI	ATTAAT	1 cut(s)	1470
XagI	CCTNNNNNAGG	1 cut(s)	1932
XapI	RAATTY	3 cut(s)	466, 1755, 1991
XceI	RCATGY	4 cut(s)	1527, 1720, 2263, 2476
XcmI	CCANNNNNNNNNTGG	2 cut(s)	2259, 2305
XhoI	CTCGAG	1 cut(s)	1592
XmnI	GAANNNNTTC	1 cut(s)	1852
XspI	CTAG	2 cut(s)	957, 2387
ZrmI	AGTACT	1 cut(s)	1814
Zsp2I	ATGCAT	4 cut(s)	316, 798, 1698, 1722

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.