pycom03g11230

Ubiquitin carboxyl-terminal hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0004829
Physical Location & Seq
Forward (+)
10899697 .. 10903918
4222 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g11230.1

Sequence Viewer

Length: 957 bp
ATGCATCAGCTGATGGAGGAGCAGCACGTGCATGGGAAACTGACCTCTAGCTTTGGAGTTCCTTTTGTATCAAGACTACCCAATTCTGCTAGCGGATCCGATATATGCAATTTATACTTAAATTTACTAAAGCCATTTCAAACACCCGCAAAAGATTCCCAAGAATGTTTTGATAGTTCAAGGGGCACCATTCTTGAAGAAGTCTCTAGAACAGAGGATTCCATACCTCCTGTTATAGACGGCGGTGTGAACCCCAGTAATGGAAATGCGGCTGACTCACCCTTTGATTCTGAATTGAAATTTTACATAACAGATGAAAAGGGAATTTCCAAGGATAAAAAGATACTAATGAAGGAGATAGTGGAGAGGGAAGAGACTAAGCGGTTGAATGTTCTTGTATGTTGGCCAAAAAAATACATCAAACAATATGACACTCGCCTTCTTAGCTCATTGCCAGAGGTTTTCAAGACTGGCCTCTTTGCAAAAAAACCTCAAGAGTCTGTTTCTCTGTACAAGTGCCTTGAAGCATTCTTGAAGGAAGAGCCTCTTGGACCAGAAGACATGTGGTATTGTCCTGCCTGCAAAAAACACTGTCAAGCCAGCAAGAAGTTGGATCTATGGAGACTGCCGGAGATCTTGGTCATTCATTTGAAGAGGTTCTCGTATAGTCGGTTTTTTAAGAACAAGCTAGAGACATATGTTGACTTCCCTATTGACGATCTTGAGTTGTCAACATATATTGCCCACAAGAGGGACCAGTTATGTAATCGTTACATGCTCTATGCAGTTAGTAATCACTTTGGAAGCATGGGAGGCGGTCATTATACTGCATTTGTCCACCATGGGGGTGATCGGTGGTATGACTTCGATGATAGCCACGTCAAACCTATCAGTCAGGACAAGATAAAGTCTTCATCTGCTTATGTTCTTTTCTACAGAAGAGTTGTGGAAGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04137 map04137
Pfam Domains
Protein Families
UCH

Protein Analysis

319

Amino Acids

36.62

Weight (kDa)

7.15

Isoelectric Point (pI)

58.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UCH PF00443 110 - 312 1.1e-35 Ubiquitin carboxyl-terminal hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 185
AciI CCGC 6 cut(s) 93, 147, 243, 269, 382, 816
AclWI GGATC 3 cut(s) 90, 103, 621
AcoI YGGCCR 1 cut(s) 404
AcsI RAATTY 3 cut(s) 121, 299, 324
AcvI CACGTG 1 cut(s) 28
AfaI GTAC 1 cut(s) 512
AfiI CCNNNNNNNGG 4 cut(s) 260, 750, 751, 844
AflIII ACRYGT 1 cut(s) 561
AgsI TTSAA 9 cut(s) 140, 180, 197, 298, 388, 466, 524, 535, 652
AjiI CACGTC 1 cut(s) 880
AjuI GAANNNNNNNTTGG 2 cut(s) 531, 563
AluBI AGCT 4 cut(s) 10, 51, 447, 688
AluI AGCT 4 cut(s) 10, 51, 447, 688
Alw26I GTCTC 4 cut(s) 208, 368, 616, 686
AlwI GGATC 3 cut(s) 90, 103, 621
AoxI GGCC 2 cut(s) 404, 472
ApeKI GCWGC 1 cut(s) 22
ApoI RAATTY 3 cut(s) 121, 299, 324
ArsI GACNNNNNNTTYG 2 cut(s) 266, 298
Asp700I GAANNNNTTC 1 cut(s) 656
AspS9I GGNCC 2 cut(s) 551, 754
AsuHPI GGTGA 2 cut(s) 270, 860
AsuNHI GCTAGC 1 cut(s) 89
AvaII GGWCC 2 cut(s) 551, 754
BaeGI GKGCMC 1 cut(s) 188
BalI TGGCCA 1 cut(s) 406
BamHI GGATCC 1 cut(s) 95
BanI GGYRCC 1 cut(s) 185
BbrPI CACGTG 1 cut(s) 28
BbsI GAAGAC 2 cut(s) 564, 903
BbvI GCAGC 1 cut(s) 34
BccI CCATC 1 cut(s) 7
BceAI ACGGC 1 cut(s) 256
BcoDI GTCTC 4 cut(s) 208, 368, 616, 686
BfaI CTAG 4 cut(s) 48, 90, 207, 689
BfmI CTRYAG 1 cut(s) 934
BglII AGATCT 1 cut(s) 633
BisI GCNGC 2 cut(s) 23, 270
BlsI GCNGC 2 cut(s) 24, 271
Bme18I GGWCC 2 cut(s) 551, 754
BmgBI CACGTC 1 cut(s) 880
BmgT120I GGNCC 2 cut(s) 551, 754
BmiI GGNNCC 3 cut(s) 97, 187, 755
BmrI ACTGGG 1 cut(s) 249
BmsI GCATC 1 cut(s) 13
BmtI GCTAGC 1 cut(s) 93
BmuI ACTGGG 1 cut(s) 249
BpiI GAAGAC 2 cut(s) 564, 903
BpuEI CTTGAG 2 cut(s) 477, 743
BsaAI YACGTR 1 cut(s) 28
BsaJI CCNNGG 2 cut(s) 330, 841
Bsc4I CCNNNNNNNGG 4 cut(s) 260, 750, 751, 844
Bse1I ACTGG 3 cut(s) 255, 475, 757
Bse3DI GCAATG 1 cut(s) 449
BseDI CCNNGG 2 cut(s) 330, 841
BseLI CCNNNNNNNGG 4 cut(s) 260, 750, 751, 844
BseMI GCAATG 1 cut(s) 449
BseNI ACTGG 3 cut(s) 255, 475, 757
BseRI GAGGAG 1 cut(s) 32
BseSI GKGCMC 1 cut(s) 188
BseXI GCAGC 1 cut(s) 34
BshFI GGCC 2 cut(s) 406, 474
BshNI GGYRCC 1 cut(s) 185
BsiSI CCGG 1 cut(s) 629
BslFI GGGAC 1 cut(s) 767
BslI CCNNNNNNNGG 4 cut(s) 260, 750, 751, 844
BsmAI GTCTC 4 cut(s) 208, 368, 616, 686
BsmFI GGGAC 1 cut(s) 767
BsmI GAATGC 1 cut(s) 527
BsnI GGCC 2 cut(s) 406, 474
Bsp1286I GDGCHC 1 cut(s) 188
Bsp1407I TGTACA 1 cut(s) 510
Bsp143I GATC 5 cut(s) 95, 613, 633, 718, 850
Bsp19I CCATGG 1 cut(s) 841
BspACI CCGC 6 cut(s) 93, 147, 243, 269, 382, 816
BspANI GGCC 2 cut(s) 406, 474
BspLI GGNNCC 3 cut(s) 97, 187, 755
BspOI GCTAGC 1 cut(s) 93
BspPI GGATC 3 cut(s) 90, 103, 621
BspQI GCTCTTC 1 cut(s) 534
BspT107I GGYRCC 1 cut(s) 185
BsrDI GCAATG 1 cut(s) 449
BsrGI TGTACA 1 cut(s) 510
BsrI ACTGG 3 cut(s) 255, 475, 757
BssECI CCNNGG 2 cut(s) 330, 841
BssMI GATC 5 cut(s) 95, 613, 633, 718, 850
BssT1I CCWWGG 2 cut(s) 330, 841
Bst4CI ACNGT 1 cut(s) 593
Bst6I CTCTTC 4 cut(s) 366, 534, 647, 934
BstAPI GCANNNNNTGC 1 cut(s) 28
BstAUI TGTACA 1 cut(s) 510
BstBAI YACGTR 1 cut(s) 28
BstC8I GCNNGC 3 cut(s) 91, 580, 601
BstDEI CTNAG 2 cut(s) 378, 443
BstDSI CCRYGG 1 cut(s) 841
BstKTI GATC 5 cut(s) 98, 616, 636, 721, 853
BstMAI GTCTC 4 cut(s) 208, 368, 616, 686
BstMBI GATC 5 cut(s) 95, 613, 633, 718, 850
BstMWI GCNNNNNNNGC 3 cut(s) 28, 444, 813
BstNSI RCATGY 2 cut(s) 565, 778
BstSFI CTRYAG 1 cut(s) 934
BstSLI GKGCMC 1 cut(s) 188
BstV1I GCAGC 1 cut(s) 34
BstV2I GAAGAC 2 cut(s) 564, 903
BstX2I RGATCY 3 cut(s) 95, 613, 633
BstYI RGATCY 3 cut(s) 95, 613, 633
BsuRI GGCC 2 cut(s) 406, 474
BtgI CCRYGG 1 cut(s) 841
BtrI CACGTC 1 cut(s) 880
BtsIMutI CAGTG 1 cut(s) 589
Cac8I GCNNGC 3 cut(s) 91, 580, 601
Cfr13I GGNCC 2 cut(s) 551, 754
Csp6I GTAC 1 cut(s) 511
CviAII CATG 5 cut(s) 32, 562, 775, 808, 842
CviQI GTAC 1 cut(s) 511
DdeI CTNAG 2 cut(s) 378, 443
DpnI GATC 5 cut(s) 97, 615, 635, 720, 852
DpnII GATC 5 cut(s) 95, 613, 633, 718, 850
EaeI YGGCCR 1 cut(s) 404
Eam1104I CTCTTC 4 cut(s) 366, 534, 647, 934
EarI CTCTTC 4 cut(s) 366, 534, 647, 934
Eco130I CCWWGG 2 cut(s) 330, 841
Eco47I GGWCC 2 cut(s) 551, 754
Eco72I CACGTG 1 cut(s) 28
EcoT14I CCWWGG 2 cut(s) 330, 841
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 2 cut(s) 330, 841
FaeI CATG 5 cut(s) 35, 565, 778, 811, 845
FalI AAGNNNNNCTT 2 cut(s) 531, 563
FaqI GGGAC 1 cut(s) 767
FatI CATG 5 cut(s) 31, 561, 774, 807, 841
FauI CCCGC 1 cut(s) 154
FauNDI CATATG 1 cut(s) 697
Fnu4HI GCNGC 2 cut(s) 23, 270
Fsp4HI GCNGC 2 cut(s) 23, 270
FspBI CTAG 4 cut(s) 48, 90, 207, 689
GluI GCNGC 2 cut(s) 23, 270
HaeIII GGCC 2 cut(s) 406, 474
HapII CCGG 1 cut(s) 629
Hin1II CATG 5 cut(s) 35, 565, 778, 811, 845
HincII GTYRAC 2 cut(s) 703, 732
HindII GTYRAC 2 cut(s) 703, 732
HinfI GANTC 5 cut(s) 155, 218, 275, 287, 497
HpaII CCGG 1 cut(s) 629
HphI GGTGA 2 cut(s) 270, 860
Hpy166II GTNNAC 4 cut(s) 250, 703, 732, 838
Hpy188I TCNGA 2 cut(s) 100, 292
Hpy188III TCNNGA 8 cut(s) 72, 194, 207, 466, 494, 532, 722, 896
Hpy8I GTNNAC 4 cut(s) 250, 703, 732, 838
HpyAV CCTTC 3 cut(s) 346, 449, 529
HpyCH4III ACNGT 1 cut(s) 593
HpyCH4IV ACGT 2 cut(s) 27, 879
HpyCH4V TGCA 7 cut(s) 4, 31, 108, 482, 582, 785, 830
HpyF10VI GCNNNNNNNGC 3 cut(s) 28, 444, 813
HpyF3I CTNAG 2 cut(s) 378, 443
HpySE526I ACGT 2 cut(s) 27, 879
Hsp92II CATG 5 cut(s) 35, 565, 778, 811, 845
Kzo9I GATC 5 cut(s) 95, 613, 633, 718, 850
LguI GCTCTTC 1 cut(s) 534
LmnI GCTCC 1 cut(s) 19
Lsp1109I GCAGC 1 cut(s) 34
LweI GCATC 1 cut(s) 13
MaeI CTAG 4 cut(s) 48, 90, 207, 689
MaeII ACGT 2 cut(s) 27, 879
MaeIII GTNAC 1 cut(s) 770
MalI GATC 5 cut(s) 97, 615, 635, 720, 852
MboI GATC 5 cut(s) 95, 613, 633, 718, 850
MboII GAAGA 7 cut(s) 209, 383, 551, 569, 664, 903, 951
MflI RGATCY 3 cut(s) 95, 613, 633
MhlI GDGCHC 1 cut(s) 188
MlsI TGGCCA 1 cut(s) 406
MluCI AATT 6 cut(s) 82, 109, 121, 293, 299, 324
MluNI TGGCCA 1 cut(s) 406
MlyI GAGTC 2 cut(s) 269, 506
MmeI TCCRAC 1 cut(s) 591
Mox20I TGGCCA 1 cut(s) 406
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 1 cut(s) 656
MscI TGGCCA 1 cut(s) 406
MseI TTAA 2 cut(s) 119, 678
MslI CAYNNNNRTG 2 cut(s) 30, 846
Msp20I TGGCCA 1 cut(s) 406
MspA1I CMGCKG 1 cut(s) 10
MspI CCGG 1 cut(s) 629
Mva1269I GAATGC 1 cut(s) 527
MwoI GCNNNNNNNGC 3 cut(s) 28, 444, 813
NcoI CCATGG 1 cut(s) 841
NdeI CATATG 1 cut(s) 697
NdeII GATC 5 cut(s) 95, 613, 633, 718, 850
NheI GCTAGC 1 cut(s) 89
NlaIII CATG 5 cut(s) 35, 565, 778, 811, 845
NlaIV GGNNCC 3 cut(s) 97, 187, 755
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 2 cut(s) 565, 778
PciI ACATGT 1 cut(s) 561
PciSI GCTCTTC 1 cut(s) 534
PctI GAATGC 1 cut(s) 527
PdmI GAANNNNTTC 1 cut(s) 656
PfeI GAWTC 3 cut(s) 155, 218, 287
PkrI GCNGC 2 cut(s) 24, 271
PleI GAGTC 2 cut(s) 269, 505
PmaCI CACGTG 1 cut(s) 28
PmlI CACGTG 1 cut(s) 28
PpsI GAGTC 2 cut(s) 269, 505
Ppu21I YACGTR 1 cut(s) 28
PscI ACATGT 1 cut(s) 561
PspCI CACGTG 1 cut(s) 28
PspN4I GGNNCC 3 cut(s) 97, 187, 755
PspPI GGNCC 2 cut(s) 551, 754
PsuI RGATCY 3 cut(s) 95, 613, 633
PvuII CAGCTG 1 cut(s) 10
RsaI GTAC 1 cut(s) 512
RsaNI GTAC 1 cut(s) 511
RseI CAYNNNNRTG 2 cut(s) 30, 846
SapI GCTCTTC 1 cut(s) 534
SaqAI TTAA 2 cut(s) 119, 678
SatI GCNGC 2 cut(s) 23, 270
Sau3AI GATC 5 cut(s) 95, 613, 633, 718, 850
Sau96I GGNCC 2 cut(s) 551, 754
SchI GAGTC 2 cut(s) 269, 506
SduI GDGCHC 1 cut(s) 188
SfaNI GCATC 1 cut(s) 13
SfcI CTRYAG 1 cut(s) 934
SinI GGWCC 2 cut(s) 551, 754
SmiMI CAYNNNNRTG 2 cut(s) 30, 846
SmlI CTYRAG 2 cut(s) 492, 722
SmoI CTYRAG 2 cut(s) 492, 722
Sse9I AATT 6 cut(s) 82, 109, 121, 293, 299, 324
SsiI CCGC 6 cut(s) 93, 147, 243, 269, 382, 816
SspMI CTAG 4 cut(s) 48, 90, 207, 689
StyI CCWWGG 2 cut(s) 330, 841
TaaI ACNGT 1 cut(s) 593
TaiI ACGT 2 cut(s) 30, 882
TaqI TCGA 1 cut(s) 867
TasI AATT 6 cut(s) 82, 109, 121, 293, 299, 324
TatI WGTACW 1 cut(s) 510
TauI GCSGC 1 cut(s) 272
TfiI GAWTC 3 cut(s) 155, 218, 287
Tru1I TTAA 2 cut(s) 119, 678
Tru9I TTAA 2 cut(s) 119, 678
TscAI CASTG 1 cut(s) 596
TseI GCWGC 1 cut(s) 22
TspDTI ATGAA 4 cut(s) 330, 365, 635, 903
TspRI CASTG 1 cut(s) 596
VpaK11BI GGWCC 2 cut(s) 551, 754
XapI RAATTY 3 cut(s) 121, 299, 324
XbaI TCTAGA 1 cut(s) 206
XceI RCATGY 2 cut(s) 565, 778
XcmI CCANNNNNNNNNTGG 2 cut(s) 561, 607
XmnI GAANNNNTTC 1 cut(s) 656
XspI CTAG 4 cut(s) 48, 90, 207, 689
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.