pycom11g14970

Ubiquitin carboxyl-terminal hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0004829
Physical Location & Seq
Forward (+)
15358152 .. 15365537
7386 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g14970.1

Sequence Viewer

Length: 1278 bp
ATGTACCTGTCGCTACCATTGCCTTCAACGATGATGCGGACAATGACTTTATCAGTGGTCAATACTTATGGATATTCTCAGCCGTCTCAATATACCATTACTGTGCCCAAGCATGGAAAATTAGAAGATCTCATCCAGGGTTTAAGTGTTGCATCCTCATTGGGGATTGATGAAACCTTTTTGGTGGCTGAGATATACAACAATCGCATTATACGCTATCTTGAGGAACCAACTGATTCATTATCTTTAATTAGCGATCAAGACAAGCTAGTTGCTTATCGGTCGATGAAAGATGTTGAGCAAATCCCTTTGGTTGTGTTCATGCATCAGCTGATGGAGGAGAAGTACATGGATGGGAAACTGACTTCAAGCTTTGGAATTCCTCTTGTGTCAAGACTACCCAATTCTGCTACTGGATCTGATATCTGCAACTTATACCTGAAATTACTTAAGCCATTTCACACACCCTCAAAAGATTCCCAAGAATGTTTTGATGGTTCAGAGGGCACCACTCTTGAAGAAGTCACTGGAACACAGGATTCCACACCTCCTGTTATAGACGGGGGTGTGAACCCCTGTAATGGAAGTGAGGCTGGCTCACCCTTTGATTTTGAATTTCAGTTTTACATAACAGATGAAAAGGGAATTTCCAAGGATAGAAAGATAGCAATGAATGATATAGTAGAGAGGGAAAAAACCAAGCGGTTAAATGTCCTTGTATGTTGGCCAAAAAAGTACATCGAACAGTATGATACTCGCCTTCTTTGCTCATTACCAGAGGTTTTCAAGACTGGCCTTTTTGCAAAAAAGCCTCAAGAGTCCGTTTCTCTGTATAAGTGCCTTGAAGCATTCTTGAAGGAAGAGCCTCTTGGACCCGAAGACATGTGGTATTGTCCTGGCTGCAAAAAGCACTGTCAAGCCAGCAAAAAGTTAGATCTATGGAGACTGCCGGAGATCTTGGTCATTCATTTAAAGCGGTTCTCATATAGCCGTTTTTTTAAGAACAAGCTGGAGACTTATGTTGATTTCCCTATTGACAATCTTGATTTGTCAACATACATTGCCCACAAGAGTGGTCAGTCATGTAATCGTTACATGCTGTATGCAGTTAGTAATCACTTTGGAAGCATGGGAGGTGGTCATTATACTGCATTTGTCCACCATGGAGGTGATCGGTGGTATGACTTTGATGATAGCCACGTTAACCCTATCAGCCAGGACAAGATAAAGTCTTCATCCGCTTATGTTCTTTTCTACAGAAGAGTTGTGGAAGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04137 map04137
Pfam Domains
Protein Families
UCH

Protein Analysis

426

Amino Acids

48.45

Weight (kDa)

5.92

Isoelectric Point (pI)

46.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UCH PF00443 283 - 419 1.3e-35 Ubiquitin carboxyl-terminal hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 506
AciI CCGC 4 cut(s) 37, 703, 976, 1239
AclWI GGATC 1 cut(s) 424
AcoI YGGCCR 1 cut(s) 725
AcsI RAATTY 3 cut(s) 378, 614, 645
AfaI GTAC 3 cut(s) 5, 347, 737
AfiI CCNNNNNNNGG 3 cut(s) 113, 162, 581
AflII CTTAAG 1 cut(s) 449
AflIII ACRYGT 1 cut(s) 882
AgsI TTSAA 7 cut(s) 27, 369, 518, 614, 787, 845, 856
AjnI CCWGG 3 cut(s) 135, 895, 1215
AjuI GAANNNNNNNTTGG 2 cut(s) 852, 884
AleI CACNNNNGTG 1 cut(s) 1071
AluBI AGCT 4 cut(s) 268, 331, 372, 1009
AluI AGCT 4 cut(s) 268, 331, 372, 1009
Alw26I GTCTC 3 cut(s) 90, 937, 1007
AlwI GGATC 1 cut(s) 424
AoxI GGCC 2 cut(s) 725, 793
ApeKI GCWGC 1 cut(s) 900
ApoI RAATTY 3 cut(s) 378, 614, 645
AspS9I GGNCC 1 cut(s) 872
AsuHPI GGTGA 2 cut(s) 591, 1181
AvaII GGWCC 1 cut(s) 872
BaeGI GKGCMC 2 cut(s) 108, 509
BalI TGGCCA 1 cut(s) 727
BanI GGYRCC 1 cut(s) 506
BbsI GAAGAC 2 cut(s) 885, 1224
BbvI GCAGC 1 cut(s) 887
BccI CCATC 3 cut(s) 328, 347, 488
BceAI ACGGC 2 cut(s) 67, 975
BciT130I CCWGG 3 cut(s) 137, 897, 1217
BcoDI GTCTC 3 cut(s) 90, 937, 1007
BfaI CTAG 1 cut(s) 269
BfmI CTRYAG 1 cut(s) 1255
BfrI CTTAAG 1 cut(s) 449
BglII AGATCT 3 cut(s) 127, 934, 954
BisI GCNGC 1 cut(s) 901
BlsI GCNGC 1 cut(s) 902
Bme1390I CCNGG 3 cut(s) 137, 897, 1217
Bme18I GGWCC 1 cut(s) 872
BmgT120I GGNCC 1 cut(s) 872
BmiI GGNNCC 3 cut(s) 228, 508, 874
BmrFI CCNGG 3 cut(s) 137, 897, 1217
BmsI GCATC 3 cut(s) 24, 161, 334
BpiI GAAGAC 2 cut(s) 885, 1224
BplI GAGNNNNNCTC 2 cut(s) 581, 613
BpmI CTGGAG 1 cut(s) 1031
BpuEI CTTGAG 2 cut(s) 242, 798
BsaJI CCNNGG 3 cut(s) 136, 651, 1162
BsaXI ACNNNNNCTCC 2 cut(s) 329, 359
Bsc4I CCNNNNNNNGG 3 cut(s) 113, 162, 581
Bse1I ACTGG 3 cut(s) 418, 532, 796
Bse3DI GCAATG 3 cut(s) 17, 675, 1059
BseBI CCWGG 3 cut(s) 137, 897, 1217
BseDI CCNNGG 3 cut(s) 136, 651, 1162
BseGI GGATG 4 cut(s) 132, 152, 358, 1235
BseLI CCNNNNNNNGG 3 cut(s) 113, 162, 581
BseMI GCAATG 3 cut(s) 17, 675, 1059
BseMII CTCAG 2 cut(s) 92, 180
BseNI ACTGG 3 cut(s) 418, 532, 796
BseRI GAGGAG 1 cut(s) 353
BseSI GKGCMC 2 cut(s) 108, 509
BseXI GCAGC 1 cut(s) 887
Bsh1285I CGRYCG 1 cut(s) 284
BshFI GGCC 2 cut(s) 727, 795
BshNI GGYRCC 1 cut(s) 506
BsiEI CGRYCG 1 cut(s) 284
BsiSI CCGG 1 cut(s) 950
BslI CCNNNNNNNGG 3 cut(s) 113, 162, 581
BsmAI GTCTC 3 cut(s) 90, 937, 1007
BsmBI CGTCTC 1 cut(s) 90
BsmI GAATGC 1 cut(s) 848
BsnI GGCC 2 cut(s) 727, 795
Bsp1286I GDGCHC 2 cut(s) 108, 509
Bsp143I GATC 6 cut(s) 127, 256, 416, 934, 954, 1171
Bsp19I CCATGG 1 cut(s) 1162
BspACI CCGC 4 cut(s) 37, 703, 976, 1239
BspANI GGCC 2 cut(s) 727, 795
BspCNI CTCAG 2 cut(s) 91, 181
BspLI GGNNCC 3 cut(s) 228, 508, 874
BspPI GGATC 1 cut(s) 424
BspQI GCTCTTC 1 cut(s) 855
BspT107I GGYRCC 1 cut(s) 506
BspTI CTTAAG 1 cut(s) 449
BsrDI GCAATG 3 cut(s) 17, 675, 1059
BsrI ACTGG 3 cut(s) 418, 532, 796
BssECI CCNNGG 3 cut(s) 136, 651, 1162
BssMI GATC 6 cut(s) 127, 256, 416, 934, 954, 1171
BssT1I CCWWGG 2 cut(s) 651, 1162
Bst2UI CCWGG 3 cut(s) 137, 897, 1217
Bst4CI ACNGT 3 cut(s) 103, 747, 914
Bst6I CTCTTC 2 cut(s) 855, 1255
BstAFI CTTAAG 1 cut(s) 449
BstC8I GCNNGC 2 cut(s) 595, 922
BstDEI CTNAG 2 cut(s) 78, 189
BstDSI CCRYGG 1 cut(s) 1162
BstF5I GGATG 4 cut(s) 132, 152, 358, 1235
BstKTI GATC 6 cut(s) 130, 259, 419, 937, 957, 1174
BstMAI GTCTC 3 cut(s) 90, 937, 1007
BstMBI GATC 6 cut(s) 127, 256, 416, 934, 954, 1171
BstMCI CGRYCG 1 cut(s) 284
BstMWI GCNNNNNNNGC 3 cut(s) 19, 213, 765
BstNI CCWGG 3 cut(s) 137, 897, 1217
BstNSI RCATGY 2 cut(s) 886, 1099
BstSCI CCNGG 3 cut(s) 135, 895, 1215
BstSFI CTRYAG 1 cut(s) 1255
BstSLI GKGCMC 2 cut(s) 108, 509
BstV1I GCAGC 1 cut(s) 887
BstV2I GAAGAC 2 cut(s) 885, 1224
BstX2I RGATCY 4 cut(s) 127, 416, 934, 954
BstXI CCANNNNNNTGG 1 cut(s) 1073
BstYI RGATCY 4 cut(s) 127, 416, 934, 954
BsuRI GGCC 2 cut(s) 727, 795
BtgI CCRYGG 1 cut(s) 1162
BtsCI GGATG 4 cut(s) 132, 152, 358, 1235
BtsIMutI CAGTG 3 cut(s) 60, 525, 910
Cac8I GCNNGC 2 cut(s) 595, 922
Cfr13I GGNCC 1 cut(s) 872
Csp6I GTAC 3 cut(s) 4, 346, 736
CviAII CATG 8 cut(s) 113, 322, 349, 883, 1083, 1096, 1129, 1163
CviQI GTAC 3 cut(s) 4, 346, 736
DdeI CTNAG 2 cut(s) 78, 189
DpnI GATC 6 cut(s) 129, 258, 418, 936, 956, 1173
DpnII GATC 6 cut(s) 127, 256, 416, 934, 954, 1171
DraI TTTAAA 1 cut(s) 972
EaeI YGGCCR 1 cut(s) 725
Eam1104I CTCTTC 2 cut(s) 855, 1255
EarI CTCTTC 2 cut(s) 855, 1255
Eco130I CCWWGG 2 cut(s) 651, 1162
Eco32I GATATC 1 cut(s) 424
Eco47I GGWCC 1 cut(s) 872
EcoRI GAATTC 1 cut(s) 378
EcoRII CCWGG 3 cut(s) 135, 895, 1215
EcoRV GATATC 1 cut(s) 424
EcoT14I CCWWGG 2 cut(s) 651, 1162
EcoT22I ATGCAT 1 cut(s) 327
ErhI CCWWGG 2 cut(s) 651, 1162
Esp3I CGTCTC 1 cut(s) 90
FaeI CATG 8 cut(s) 116, 325, 352, 886, 1086, 1099, 1132, 1166
FalI AAGNNNNNCTT 2 cut(s) 852, 884
FatI CATG 8 cut(s) 112, 321, 348, 882, 1082, 1095, 1128, 1162
Fnu4HI GCNGC 1 cut(s) 901
FokI GGATG 4 cut(s) 119, 139, 365, 1222
Fsp4HI GCNGC 1 cut(s) 901
FspBI CTAG 1 cut(s) 269
GluI GCNGC 1 cut(s) 901
GsuI CTGGAG 1 cut(s) 1031
HaeIII GGCC 2 cut(s) 727, 795
HapII CCGG 1 cut(s) 950
Hin1II CATG 8 cut(s) 116, 325, 352, 886, 1086, 1099, 1132, 1166
HincII GTYRAC 2 cut(s) 1053, 1204
HindII GTYRAC 2 cut(s) 1053, 1204
HindIII AAGCTT 1 cut(s) 370
HinfI GANTC 4 cut(s) 236, 476, 539, 818
HpaI GTTAAC 1 cut(s) 1204
HpaII CCGG 1 cut(s) 950
HphI GGTGA 2 cut(s) 591, 1181
Hpy166II GTNNAC 4 cut(s) 571, 1053, 1159, 1204
Hpy188I TCNGA 2 cut(s) 421, 502
Hpy188III TCNNGA 8 cut(s) 221, 260, 393, 515, 787, 815, 853, 1043
Hpy8I GTNNAC 4 cut(s) 571, 1053, 1159, 1204
HpyAV CCTTC 3 cut(s) 33, 770, 850
HpyCH4III ACNGT 3 cut(s) 103, 747, 914
HpyCH4IV ACGT 1 cut(s) 1200
HpyCH4V TGCA 7 cut(s) 152, 325, 429, 803, 903, 1106, 1151
HpyF10VI GCNNNNNNNGC 3 cut(s) 19, 213, 765
HpyF3I CTNAG 2 cut(s) 78, 189
HpySE526I ACGT 1 cut(s) 1200
Hsp92II CATG 8 cut(s) 116, 325, 352, 886, 1086, 1099, 1132, 1166
KspAI GTTAAC 1 cut(s) 1204
Kzo9I GATC 6 cut(s) 127, 256, 416, 934, 954, 1171
LguI GCTCTTC 1 cut(s) 855
Lsp1109I GCAGC 1 cut(s) 887
LweI GCATC 3 cut(s) 24, 161, 334
MaeI CTAG 1 cut(s) 269
MaeII ACGT 1 cut(s) 1200
MaeIII GTNAC 2 cut(s) 523, 1091
MalI GATC 6 cut(s) 129, 258, 418, 936, 956, 1173
MboI GATC 6 cut(s) 127, 256, 416, 934, 954, 1171
MboII GAAGA 6 cut(s) 137, 530, 872, 890, 1224, 1272
MflI RGATCY 4 cut(s) 127, 416, 934, 954
MhlI GDGCHC 2 cut(s) 108, 509
MlsI TGGCCA 1 cut(s) 727
MluCI AATT 7 cut(s) 119, 249, 378, 403, 443, 614, 645
MluNI TGGCCA 1 cut(s) 727
MlyI GAGTC 1 cut(s) 827
Mox20I TGGCCA 1 cut(s) 727
Mph1103I ATGCAT 1 cut(s) 327
MscI TGGCCA 1 cut(s) 727
MseI TTAA 7 cut(s) 143, 248, 450, 707, 971, 999, 1203
MslI CAYNNNNRTG 3 cut(s) 101, 1071, 1167
Msp20I TGGCCA 1 cut(s) 727
MspA1I CMGCKG 1 cut(s) 331
MspCI CTTAAG 1 cut(s) 449
MspI CCGG 1 cut(s) 950
MspR9I CCNGG 3 cut(s) 137, 897, 1217
Mva1269I GAATGC 1 cut(s) 848
MvaI CCWGG 3 cut(s) 137, 897, 1217
MwoI GCNNNNNNNGC 3 cut(s) 19, 213, 765
NcoI CCATGG 1 cut(s) 1162
NdeII GATC 6 cut(s) 127, 256, 416, 934, 954, 1171
NlaIII CATG 8 cut(s) 116, 325, 352, 886, 1086, 1099, 1132, 1166
NlaIV GGNNCC 3 cut(s) 228, 508, 874
NmuCI GTSAC 1 cut(s) 523
NsiI ATGCAT 1 cut(s) 327
NspI RCATGY 2 cut(s) 886, 1099
OliI CACNNNNGTG 1 cut(s) 1071
PciI ACATGT 1 cut(s) 882
PciSI GCTCTTC 1 cut(s) 855
PctI GAATGC 1 cut(s) 848
PfeI GAWTC 3 cut(s) 236, 476, 539
PkrI GCNGC 1 cut(s) 902
PleI GAGTC 1 cut(s) 826
PpsI GAGTC 1 cut(s) 826
PscI ACATGT 1 cut(s) 882
Psp6I CCWGG 3 cut(s) 135, 895, 1215
PspGI CCWGG 3 cut(s) 135, 895, 1215
PspN4I GGNNCC 3 cut(s) 228, 508, 874
PspPI GGNCC 1 cut(s) 872
PsuI RGATCY 4 cut(s) 127, 416, 934, 954
PvuII CAGCTG 1 cut(s) 331
RsaI GTAC 3 cut(s) 5, 347, 737
RsaNI GTAC 3 cut(s) 4, 346, 736
RseI CAYNNNNRTG 3 cut(s) 101, 1071, 1167
SapI GCTCTTC 1 cut(s) 855
SaqAI TTAA 7 cut(s) 143, 248, 450, 707, 971, 999, 1203
SatI GCNGC 1 cut(s) 901
Sau3AI GATC 6 cut(s) 127, 256, 416, 934, 954, 1171
Sau96I GGNCC 1 cut(s) 872
SchI GAGTC 1 cut(s) 827
ScrFI CCNGG 3 cut(s) 137, 897, 1217
SduI GDGCHC 2 cut(s) 108, 509
SfaNI GCATC 3 cut(s) 24, 161, 334
SfcI CTRYAG 1 cut(s) 1255
SinI GGWCC 1 cut(s) 872
SmiMI CAYNNNNRTG 3 cut(s) 101, 1071, 1167
SmlI CTYRAG 3 cut(s) 221, 449, 813
SmoI CTYRAG 3 cut(s) 221, 449, 813
Sse9I AATT 7 cut(s) 119, 249, 378, 403, 443, 614, 645
SsiI CCGC 4 cut(s) 37, 703, 976, 1239
SspMI CTAG 1 cut(s) 269
StyD4I CCNGG 3 cut(s) 135, 895, 1215
StyI CCWWGG 2 cut(s) 651, 1162
TaaI ACNGT 3 cut(s) 103, 747, 914
TaiI ACGT 1 cut(s) 1203
TaqI TCGA 2 cut(s) 284, 741
TaqII GACCGA 1 cut(s) 270
TasI AATT 7 cut(s) 119, 249, 378, 403, 443, 614, 645
TatI WGTACW 2 cut(s) 345, 735
TfiI GAWTC 3 cut(s) 236, 476, 539
Tru1I TTAA 7 cut(s) 143, 248, 450, 707, 971, 999, 1203
Tru9I TTAA 7 cut(s) 143, 248, 450, 707, 971, 999, 1203
TscAI CASTG 3 cut(s) 60, 532, 917
TseFI GTSAC 1 cut(s) 523
TseI GCWGC 1 cut(s) 900
Tsp45I GTSAC 1 cut(s) 523
TspDTI ATGAA 8 cut(s) 186, 228, 302, 310, 651, 686, 956, 1224
TspGWI ACGGA 1 cut(s) 811
TspRI CASTG 3 cut(s) 60, 532, 917
Vha464I CTTAAG 1 cut(s) 449
VpaK11BI GGWCC 1 cut(s) 872
XapI RAATTY 3 cut(s) 378, 614, 645
XceI RCATGY 2 cut(s) 886, 1099
XspI CTAG 1 cut(s) 269
Zsp2I ATGCAT 1 cut(s) 327
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.