Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr5

Orthogroup ID

OG0004829

Physical Location & Seq

Genomic Coordinates Forward (+)

48307018 .. 48318252

Gene Structure 11235 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw5G030460.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2109 bp

ATGAAGATCATAAACAACATTTTCAACTCAGATCTCGTGTTCAATCTCAGGAGAGAGGATGATGCTGTGCACAATGCTGAAAATGCCGAGGTCGGTGTATCGGGCCGGGACTATGCGTTGGTCCCCGGAGAAATGTGGGTTCAGGCGCTCAGATGGCATAGTGATCCTAAAGCTGCAGTTAAGGATGGTAGGAGTTTTTCGGCTGCAGAAGATGGCAAGACCGATGTCTATCCCCTACAACTGAGGCTTTCTGTTCCACGAGAAACAAATTCATTGGGAGTAAGAATTACCAAAAAGGATAATGCAGTTGAGTCCTTCAAAAGAGCCTGCAAAATCTTCAGTTTGGAGTCTGACTTGTTACGCATCTGGGACTTTTCCGGCCAGACTAACTTATTTTTTACAAATGACAAAACCAAGTTTCCAAGAGACTTGCAACGGCAGTCAGATGAGATCCTACTGGAGTTGCAAGTTTATGGATTGTCAGATTCCATCAAGTGCAGGGAAGGGAAAAAAGATGAGATGGCCATGCAACATTCTACCATTGGAAGTTCTTGTTCTGGTTCTTCAGTTGTGATGAATGGTTCTGCTGGCAATATGCATTCCAATTTCATTCGTTCATTCTGGGGAAGTTCTTGTGAAGCTGGTTCTTTAGGGTTGACAGGATTGCAAAACCTTGGAAATACTTGTTTCATGAATAGCTCCATCCAGTGCTTAGCCCATACACCAAAGCTTGTTGACTATTTTCTTGGAGATTATGCAAGAGAAATAAATCAGGAAAACCCATTGGGCATGGATGGGGAGATTGCTTTAGCATTTGGAGATCTGTTAAGGAAGTTATGGGCTCCTGGAGCAACTCCTGTGCCTCCAAGGACCTTTAAATCAAAACTTGCTCGTTTTGCTCCTCAGTTTAATGGCTTTAATCAACATGATTCACAAGAACTCCTGGCCTTTTTGTTGGATGGACTTCATGAAGATCTTAATCGTGTAAAATGCAAGCCTTATGTAGAAGTCAAGGATGGTGATGATCGACCGGATGAAGAGGTAGCTGATGAATATTGGCGAAATCATTTAGCCCGCAATGACTCAATCATTGTTGATGTGTGCCAAGGTCAATATAAATCAACATTAGTTTGTCCTGTTTGCAAAAAGGTCTCTGTTACATTTGATCCGTTTATGTACCTGTCACTACCATTACCTTCAACAATGATGCGGACAATGACTTTAACAGTTGTCAACACTGATGGAAGTTCGAAGCCATCTGAATATACCATTAATGTGCCCAAGCATGGAAAGTTTGATGATCTCATCCGCAGTTTGAGTGTTGCATGCTCACTTGGAGTTGATGAAACCTTTTTAGTGGCCGAGATATACAACAATCGCATTATACGTTATCTCGAGGAGCCTGCTGATTCGTTATCCTTAATTAGAGATGGTGACCTGTTGGTTGCTTATCGGTTTATGAAAGATGTTGAGGAAGTACCTCTGGTCGTTTTCATGCATCAACAAATGGAGCAGGACATGCATGGGAAGTTGAGTTCAAGCTGGAAACCGTTTGGAATTCCTCTTGTATCAAGACTTTGTACTTCGGCAACTGGATCTGATATCTGCTATCAGTACTTAAACTTAGTTAAGCCATTTCAAATACCTGGAAAAGATTCCCAAGCACTTGTTGATAATTCACAGGGCACCGCTCCTATAGAGGCCACTGGAGCAGAGGGTACCCCACCTCCTGTTTCAGGTGGGGATGTGAACCTCTGTTATGAAAATGGGGCTGACTCACCGTTGGATTGTGAATTTCAGTTTTATATAACAGATGAAAAGGGTATTCAAAAGGAGAAAAAGATAGTAATGAATGAGATGGTCGAGAGGAAAATGTCGGAGCATTTATATGTTGCAGTATGTTGGCCAGAAAAATACGTTGAGCAGTATGATACTCGCGTTCTGAGCTCATTGCCGTCGGTTTTCAAGCCTGGCCTTTTTCCGAAAAAGCCTCAGGAGACTGTTTCTCTATATAAGTGCCTTGAAGCATTCTTGAAGGAGGAGCCTCTTGGGCCAGAAGACATGTGGTCAGTATTGTCCTGGCTGCAAAAAGCATTGCCAAGCCAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko04137 map04137

Pfam Domains

Protein Families

UCH

Protein Analysis

702

Amino Acids

78.71

Weight (kDa)

5.18

Isoelectric Point (pI)

41.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ubiquitin_UBP8	PF25242	81 - 135	3.4e-30	UBP8-like, ubiquitin-like domain
UCH	PF00443	220 - 502	2e-37	Ubiquitin carboxyl-terminal hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0004829)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G22030 AT5G22030
fragaria_vesca	FvH4_3g26140 FvH4_3g26140 FvH4_3g26140 FvH4_3g26140 FvH4_3g26140 FvH4_3g26140 FvH4_3g26140
malus_domestica	MD03G1159600.v1.1 MD11G1174900.v1.1
prunus_persica	Prupe.6G147300_v2.0.a1 Prupe.6G147300_v2.0.a1 Prupe.6G147300_v2.0.a1 Prupe.6G147300_v2.0.a1
pyrus_communis	pycom03g11220 pycom03g11230 pycom11g14960 pycom11g14970
rosa_chinensis	RchiOBHm_Chr5g0049121
rosa_laevigata	RLG00000034590
rosa_multiflora	Rmu_sc0000530.1_g000038 Rmu_sc0000530.1_g000039
rosa_roxburghii	Rroxscaffold_1G00032700
rosa_rugosa	Rorug05G0243300 Rorug05G0243300
rosa_samantha	Rh5AG322700 Rh5BG333300 Rh5CG358200 Rh5DG344700
rosa_wichuraiana	Rw5G030460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	1718
AccB1I	GGYRCC	2 cut(s)	1685, 1718
AccBSI	CCGCTC	1 cut(s)	1691
AccII	CGCG	1 cut(s)	1938
AciI	CCGC	4 cut(s)	1075, 1210, 1309, 1689
AclWI	GGATC	4 cut(s)	158, 445, 1160, 1603
AcoI	YGGCCR	4 cut(s)	379, 522, 1359, 1904
AcsI	RAATTY	3 cut(s)	268, 1557, 1793
AcuI	CTGAAG	2 cut(s)	322, 549
AfaI	GTAC	5 cut(s)	1178, 1479, 1582, 1616, 1720
AfiI	CCNNNNNNNGG	2 cut(s)	1286, 1736
AflIII	ACRYGT	1 cut(s)	2061
AhdI	GACNNNNNGTC	1 cut(s)	2065
AjnI	CCWGG	5 cut(s)	844, 942, 1645, 1969, 2078
AloI	GAACNNNNNNTCC	2 cut(s)	1519, 1551
AluBI	AGCT	7 cut(s)	173, 641, 699, 730, 1046, 1542, 1947
AluI	AGCT	7 cut(s)	173, 641, 699, 730, 1046, 1542, 1947
Alw21I	GWGCWC	2 cut(s)	72, 1949
Alw26I	GTCTC	3 cut(s)	420, 1156, 1991
Alw44I	GTGCAC	1 cut(s)	68
AlwI	GGATC	4 cut(s)	158, 445, 1160, 1603
Ama87I	CYCGRG	1 cut(s)	1394
AoxI	GGCC	9 cut(s)	103, 379, 522, 945, 1359, 1701, 1904, 1972, 2051
ApaLI	GTGCAC	1 cut(s)	68
ApeKI	GCWGC	3 cut(s)	173, 203, 2083
ApoI	RAATTY	3 cut(s)	268, 1557, 1793
AseI	ATTAAT	1 cut(s)	1272
Asp700I	GAANNNNTTC	1 cut(s)	1654
Asp718I	GGTACC	1 cut(s)	1718
AspLEI	GCGC	1 cut(s)	148
AspS9I	GGNCC	4 cut(s)	103, 121, 870, 2051
AsuC2I	CCSGG	2 cut(s)	107, 126
AsuHPI	GGTGA	3 cut(s)	1031, 1445, 1770
AsuII	TTCGAA	1 cut(s)	1250
AvaI	CYCGRG	1 cut(s)	1394
AvaII	GGWCC	2 cut(s)	121, 870
AxyI	CCTNAGG	1 cut(s)	1992
BaeGI	GKGCMC	3 cut(s)	72, 1281, 1688
BaeI	ACNNNNGTAYC	2 cut(s)	1923, 1956
BalI	TGGCCA	2 cut(s)	524, 1906
BanI	GGYRCC	2 cut(s)	1685, 1718
BanII	GRGCYC	2 cut(s)	844, 1949
BauI	CACGAG	2 cut(s)	35, 258
BbsI	GAAGAC	1 cut(s)	2064
Bbv12I	GWGCWC	2 cut(s)	72, 1949
BbvI	GCAGC	3 cut(s)	160, 190, 2070
BceAI	ACGGC	2 cut(s)	452, 1939
BcgI	CGANNNNNNTGC	2 cut(s)	1385, 1419
BciT130I	CCWGG	5 cut(s)	846, 944, 1647, 1971, 2080
BcnI	CCSGG	2 cut(s)	107, 126
BcoDI	GTCTC	3 cut(s)	420, 1156, 1991
BfmI	CTRYAG	3 cut(s)	174, 204, 1695
BfoI	RGCGCY	1 cut(s)	149
BglI	GCCNNNNNGGC	1 cut(s)	2050
BglII	AGATCT	3 cut(s)	31, 820, 973
BisI	GCNGC	3 cut(s)	174, 204, 2084
BlpI	GCTNAGC	1 cut(s)	712
BlsI	GCNGC	3 cut(s)	175, 205, 2085
BmcAI	AGTACT	1 cut(s)	1616
Bme1390I	CCNGG	7 cut(s)	107, 126, 846, 944, 1647, 1971, 2080
Bme18I	GGWCC	2 cut(s)	121, 870
BmeRI	GACNNNNNGTC	1 cut(s)	2065
BmeT110I	CYCGRG	1 cut(s)	1394
BmgT120I	GGNCC	4 cut(s)	103, 121, 870, 2051
BmiI	GGNNCC	6 cut(s)	123, 843, 1401, 1687, 1720, 2043
BmrFI	CCNGG	7 cut(s)	107, 126, 846, 944, 1647, 1971, 2080
BmsI	GCATC	4 cut(s)	52, 372, 1197, 1507
BoxI	GACNNNNGTC	1 cut(s)	224
BpiI	GAAGAC	1 cut(s)	2064
BpmI	CTGGAG	3 cut(s)	479, 867, 1728
Bpu1102I	GCTNAGC	1 cut(s)	712
Bpu14I	TTCGAA	1 cut(s)	1250
BpuMI	CCSGG	2 cut(s)	107, 126
BsaBI	GATNNNNATC	2 cut(s)	228, 978
BsaI	GGTCTC	1 cut(s)	1156
BsaJI	CCNNGG	5 cut(s)	87, 124, 673, 866, 1105
BsaWI	WCCGGW	1 cut(s)	1030
BsaXI	ACNNNNNCTCC	4 cut(s)	924, 954, 1711, 1741
Bsc4I	CCNNNNNNNGG	2 cut(s)	1286, 1736
Bse1I	ACTGG	5 cut(s)	462, 706, 1597, 1711, 2104
Bse21I	CCTNAGG	1 cut(s)	1992
Bse3DI	GCAATG	3 cut(s)	1084, 1949, 2093
Bse8I	GATNNNNATC	2 cut(s)	228, 978
BseBI	CCWGG	5 cut(s)	846, 944, 1647, 1971, 2080
BseDI	CCNNGG	5 cut(s)	87, 124, 673, 866, 1105
BseGI	GGATG	9 cut(s)	64, 190, 702, 799, 964, 1021, 1039, 1305, 1750
BseJI	GATNNNNATC	2 cut(s)	228, 978
BseLI	CCNNNNNNNGG	2 cut(s)	1286, 1736
BseMI	GCAATG	3 cut(s)	1084, 1949, 2093
BseMII	CTCAG	7 cut(s)	42, 61, 163, 233, 917, 1934, 2006
BseNI	ACTGG	5 cut(s)	462, 706, 1597, 1711, 2104
BseRI	GAGGAG	3 cut(s)	891, 1412, 2054
BseSI	GKGCMC	3 cut(s)	72, 1281, 1688
BseXI	GCAGC	3 cut(s)	160, 190, 2070
BsgI	GTGCAG	1 cut(s)	517
Bsh1236I	CGCG	1 cut(s)	1938
Bsh1285I	CGRYCG	1 cut(s)	1031
BshFI	GGCC	9 cut(s)	105, 381, 524, 947, 1361, 1703, 1906, 1974, 2053
BshNI	GGYRCC	2 cut(s)	1685, 1718
BsiEI	CGRYCG	1 cut(s)	1031
BsiHKAI	GWGCWC	2 cut(s)	72, 1949
BsiHKCI	CYCGRG	1 cut(s)	1394
BsiSI	CCGG	4 cut(s)	106, 126, 378, 1031
BslFI	GGGAC	3 cut(s)	107, 122, 383
BslI	CCNNNNNNNGG	2 cut(s)	1286, 1736
BsmAI	GTCTC	3 cut(s)	420, 1156, 1991
BsmFI	GGGAC	3 cut(s)	107, 122, 383
BsmI	GAATGC	2 cut(s)	598, 2027
BsnI	GGCC	9 cut(s)	105, 381, 524, 947, 1361, 1703, 1906, 1974, 2053
Bso31I	GGTCTC	1 cut(s)	1156
BsoBI	CYCGRG	1 cut(s)	1394
Bsp119I	TTCGAA	1 cut(s)	1250
Bsp1286I	GDGCHC	5 cut(s)	72, 844, 1281, 1688, 1949
Bsp1720I	GCTNAGC	1 cut(s)	712
BspACI	CCGC	4 cut(s)	1075, 1210, 1309, 1689
BspANI	GGCC	9 cut(s)	105, 381, 524, 947, 1361, 1703, 1906, 1974, 2053
BspCNI	CTCAG	7 cut(s)	41, 60, 162, 234, 916, 1935, 2005
BspFNI	CGCG	1 cut(s)	1938
BspHI	TCATGA	2 cut(s)	690, 967
BspLI	GGNNCC	6 cut(s)	123, 843, 1401, 1687, 1720, 2043
BspMAI	CTGCAG	2 cut(s)	178, 208
BspPI	GGATC	4 cut(s)	158, 445, 1160, 1603
BspT104I	TTCGAA	1 cut(s)	1250
BspT107I	GGYRCC	2 cut(s)	1685, 1718
BspTNI	GGTCTC	1 cut(s)	1156
BsrBI	CCGCTC	1 cut(s)	1691
BsrDI	GCAATG	3 cut(s)	1084, 1949, 2093
BsrI	ACTGG	5 cut(s)	462, 706, 1597, 1711, 2104
BssECI	CCNNGG	5 cut(s)	87, 124, 673, 866, 1105
BssSI	CACGAG	2 cut(s)	35, 258
BssT1I	CCWWGG	3 cut(s)	673, 866, 1105
Bst2BI	CACGAG	2 cut(s)	35, 258
Bst2UI	CCWGG	5 cut(s)	846, 944, 1647, 1971, 2080
Bst4CI	ACNGT	4 cut(s)	1228, 1551, 1782, 2002
Bst6I	CTCTTC	1 cut(s)	1032
BstAPI	GCANNNNNTGC	1 cut(s)	1519
BstBI	TTCGAA	1 cut(s)	1250
BstC8I	GCNNGC	6 cut(s)	328, 589, 995, 1075, 1327, 1404
BstDEI	CTNAG	9 cut(s)	28, 47, 149, 242, 712, 903, 1624, 1943, 1992
BstEII	GGTNACC	1 cut(s)	1433
BstENI	CCTNNNNNAGG	1 cut(s)	1734
BstF5I	GGATG	9 cut(s)	64, 190, 702, 799, 964, 1021, 1039, 1305, 1750
BstFNI	CGCG	1 cut(s)	1938
BstH2I	RGCGCY	1 cut(s)	149
BstHHI	GCGC	1 cut(s)	148
BstMAI	GTCTC	3 cut(s)	420, 1156, 1991
BstMCI	CGRYCG	1 cut(s)	1031
BstMWI	GCNNNNNNNGC	8 cut(s)	83, 154, 848, 896, 1519, 1709, 1944, 2050
BstNI	CCWGG	5 cut(s)	846, 944, 1647, 1971, 2080
BstNSI	RCATGY	3 cut(s)	1329, 1522, 2065
BstPAI	GACNNNNGTC	1 cut(s)	224
BstPI	GGTNACC	1 cut(s)	1433
BstSCI	CCNGG	7 cut(s)	105, 124, 844, 942, 1645, 1969, 2078
BstSFI	CTRYAG	3 cut(s)	174, 204, 1695
BstSLI	GKGCMC	3 cut(s)	72, 1281, 1688
BstUI	CGCG	1 cut(s)	1938
BstV1I	GCAGC	3 cut(s)	160, 190, 2070
BstV2I	GAAGAC	1 cut(s)	2064
BstX2I	RGATCY	5 cut(s)	31, 450, 820, 973, 1595
BstYI	RGATCY	5 cut(s)	31, 450, 820, 973, 1595
Bsu36I	CCTNAGG	1 cut(s)	1992
BsuRI	GGCC	9 cut(s)	105, 381, 524, 947, 1361, 1703, 1906, 1974, 2053
BtsCI	GGATG	9 cut(s)	64, 190, 702, 799, 964, 1021, 1039, 1305, 1750
BtsIMutI	CAGTG	3 cut(s)	713, 1236, 1704
Cac8I	GCNNGC	6 cut(s)	328, 589, 995, 1075, 1327, 1404
CciI	TCATGA	2 cut(s)	690, 967
CfoI	GCGC	1 cut(s)	148
Cfr13I	GGNCC	4 cut(s)	103, 121, 870, 2051
Csp6I	GTAC	5 cut(s)	1177, 1478, 1581, 1615, 1719
CviQI	GTAC	5 cut(s)	1177, 1478, 1581, 1615, 1719
DdeI	CTNAG	9 cut(s)	28, 47, 149, 242, 712, 903, 1624, 1943, 1992
DraI	TTTAAA	1 cut(s)	877
DriI	GACNNNNNGTC	1 cut(s)	2065
EaeI	YGGCCR	4 cut(s)	379, 522, 1359, 1904
Eam1104I	CTCTTC	1 cut(s)	1032
Eam1105I	GACNNNNNGTC	1 cut(s)	2065
EarI	CTCTTC	1 cut(s)	1032
Ecl136II	GAGCTC	1 cut(s)	1947
Eco130I	CCWWGG	3 cut(s)	673, 866, 1105
Eco24I	GRGCYC	2 cut(s)	844, 1949
Eco31I	GGTCTC	1 cut(s)	1156
Eco32I	GATATC	1 cut(s)	1603
Eco47I	GGWCC	2 cut(s)	121, 870
Eco53kI	GAGCTC	1 cut(s)	1947
Eco57I	CTGAAG	2 cut(s)	322, 549
Eco81I	CCTNAGG	1 cut(s)	1992
Eco88I	CYCGRG	1 cut(s)	1394
Eco91I	GGTNACC	1 cut(s)	1433
EcoICRI	GAGCTC	1 cut(s)	1947
EcoNI	CCTNNNNNAGG	1 cut(s)	1734
EcoO109I	RGGNCCY	1 cut(s)	870
EcoO65I	GGTNACC	1 cut(s)	1433
EcoRI	GAATTC	1 cut(s)	1557
EcoRII	CCWGG	5 cut(s)	844, 942, 1645, 1969, 2078
EcoRV	GATATC	1 cut(s)	1603
EcoT14I	CCWWGG	3 cut(s)	673, 866, 1105
EcoT22I	ATGCAT	3 cut(s)	600, 1500, 1524
EcoT38I	GRGCYC	2 cut(s)	844, 1949
ErhI	CCWWGG	3 cut(s)	673, 866, 1105
FaqI	GGGAC	3 cut(s)	107, 122, 383
FauI	CCCGC	1 cut(s)	1082
Fnu4HI	GCNGC	3 cut(s)	174, 204, 2084
FokI	GGATG	9 cut(s)	71, 197, 689, 806, 971, 1028, 1046, 1292, 1757
FriOI	GRGCYC	2 cut(s)	844, 1949
Fsp4HI	GCNGC	3 cut(s)	174, 204, 2084
GlaI	GCGC	1 cut(s)	147
GluI	GCNGC	3 cut(s)	174, 204, 2084
GsuI	CTGGAG	3 cut(s)	479, 867, 1728
HaeII	RGCGCY	1 cut(s)	149
HaeIII	GGCC	9 cut(s)	105, 381, 524, 947, 1361, 1703, 1906, 1974, 2053
HapII	CCGG	4 cut(s)	106, 126, 378, 1031
HhaI	GCGC	1 cut(s)	148
Hin6I	GCGC	1 cut(s)	146
HinP1I	GCGC	1 cut(s)	146
HincII	GTYRAC	3 cut(s)	657, 736, 1234
HindII	GTYRAC	3 cut(s)	657, 736, 1234
HindIII	AAGCTT	1 cut(s)	728
HinfI	GANTC	8 cut(s)	311, 347, 485, 929, 1082, 1409, 1655, 1775
HpaII	CCGG	4 cut(s)	106, 126, 378, 1031
HphI	GGTGA	3 cut(s)	1031, 1445, 1770
Hpy166II	GTNNAC	5 cut(s)	70, 657, 736, 1234, 1750
Hpy188III	TCNNGA	9 cut(s)	49, 691, 773, 968, 1394, 1572, 1864, 1994, 2032
Hpy8I	GTNNAC	5 cut(s)	70, 657, 736, 1234, 1750
Hpy99I	CGWCG	1 cut(s)	1960
HpyAV	CCTTC	4 cut(s)	325, 497, 1206, 2029
HpyCH4III	ACNGT	4 cut(s)	1228, 1551, 1782, 2002
HpyCH4IV	ACGT	2 cut(s)	1387, 1917
HpyF10VI	GCNNNNNNNGC	8 cut(s)	83, 154, 848, 896, 1519, 1709, 1944, 2050
HpyF3I	CTNAG	9 cut(s)	28, 47, 149, 242, 712, 903, 1624, 1943, 1992
HpySE526I	ACGT	2 cut(s)	1387, 1917
HspAI	GCGC	1 cut(s)	146
KpnI	GGTACC	1 cut(s)	1722
Lsp1109I	GCAGC	3 cut(s)	160, 190, 2070
LweI	GCATC	4 cut(s)	52, 372, 1197, 1507
MaeII	ACGT	2 cut(s)	1387, 1917
MaeIII	GTNAC	4 cut(s)	357, 1156, 1182, 1433
MbiI	CCGCTC	1 cut(s)	1691
MboII	GAAGA	7 cut(s)	16, 221, 328, 555, 983, 1049, 2069
MflI	RGATCY	5 cut(s)	31, 450, 820, 973, 1595
MhlI	GDGCHC	5 cut(s)	72, 844, 1281, 1688, 1949
MlsI	TGGCCA	2 cut(s)	524, 1906
MluCI	AATT	7 cut(s)	268, 285, 604, 1422, 1557, 1675, 1793
MluNI	TGGCCA	2 cut(s)	524, 1906
MlyI	GAGTC	4 cut(s)	320, 356, 1076, 1769
MmeI	TCCRAC	3 cut(s)	936, 1764, 1857
Mox20I	TGGCCA	2 cut(s)	524, 1906
Mph1103I	ATGCAT	3 cut(s)	600, 1500, 1524
MroXI	GAANNNNTTC	1 cut(s)	1654
MscI	TGGCCA	2 cut(s)	524, 1906
MslI	CAYNNNNRTG	2 cut(s)	1274, 1887
Msp20I	TGGCCA	2 cut(s)	524, 1906
MspI	CCGG	4 cut(s)	106, 126, 378, 1031
MspR9I	CCNGG	7 cut(s)	107, 126, 846, 944, 1647, 1971, 2080
Mva1269I	GAATGC	2 cut(s)	598, 2027
MvaI	CCWGG	5 cut(s)	846, 944, 1647, 1971, 2080
MvnI	CGCG	1 cut(s)	1938
MwoI	GCNNNNNNNGC	8 cut(s)	83, 154, 848, 896, 1519, 1709, 1944, 2050
NciI	CCSGG	2 cut(s)	107, 126
NlaIV	GGNNCC	6 cut(s)	123, 843, 1401, 1687, 1720, 2043
NmeAIII	GCCGAG	2 cut(s)	112, 1387
NmuCI	GTSAC	2 cut(s)	1182, 1433
NsiI	ATGCAT	3 cut(s)	600, 1500, 1524
NspI	RCATGY	3 cut(s)	1329, 1522, 2065
NspV	TTCGAA	1 cut(s)	1250
PaeI	GCATGC	1 cut(s)	1329
PaeR7I	CTCGAG	1 cut(s)	1394
PagI	TCATGA	2 cut(s)	690, 967
PciI	ACATGT	1 cut(s)	2061
PcsI	WCGNNNNNNNCGW	1 cut(s)	1384
PctI	GAATGC	2 cut(s)	598, 2027
PdmI	GAANNNNTTC	1 cut(s)	1654
PfeI	GAWTC	4 cut(s)	485, 929, 1409, 1655
PfoI	TCCNGGA	1 cut(s)	844
PkrI	GCNGC	3 cut(s)	175, 205, 2085
PleI	GAGTC	4 cut(s)	319, 355, 1076, 1769
PpsI	GAGTC	4 cut(s)	319, 355, 1076, 1769
PpuMI	RGGWCCY	1 cut(s)	870
PscI	ACATGT	1 cut(s)	2061
PshAI	GACNNNNGTC	1 cut(s)	224
PshBI	ATTAAT	1 cut(s)	1272
Psp124BI	GAGCTC	1 cut(s)	1949
Psp5II	RGGWCCY	1 cut(s)	870
Psp6I	CCWGG	5 cut(s)	844, 942, 1645, 1969, 2078
PspEI	GGTNACC	1 cut(s)	1433
PspGI	CCWGG	5 cut(s)	844, 942, 1645, 1969, 2078
PspN4I	GGNNCC	6 cut(s)	123, 843, 1401, 1687, 1720, 2043
PspPI	GGNCC	4 cut(s)	103, 121, 870, 2051
PspPPI	RGGWCCY	1 cut(s)	870
PstI	CTGCAG	2 cut(s)	178, 208
PsuI	RGATCY	5 cut(s)	31, 450, 820, 973, 1595
RsaI	GTAC	5 cut(s)	1178, 1479, 1582, 1616, 1720
RsaNI	GTAC	5 cut(s)	1177, 1478, 1581, 1615, 1719
RseI	CAYNNNNRTG	2 cut(s)	1274, 1887
SacI	GAGCTC	1 cut(s)	1949
SatI	GCNGC	3 cut(s)	174, 204, 2084
Sau96I	GGNCC	4 cut(s)	103, 121, 870, 2051
ScaI	AGTACT	1 cut(s)	1616
SchI	GAGTC	4 cut(s)	320, 356, 1076, 1769
ScrFI	CCNGG	7 cut(s)	107, 126, 846, 944, 1647, 1971, 2080
SduI	GDGCHC	5 cut(s)	72, 844, 1281, 1688, 1949
SfaNI	GCATC	4 cut(s)	52, 372, 1197, 1507
SfcI	CTRYAG	3 cut(s)	174, 204, 1695
Sfr274I	CTCGAG	1 cut(s)	1394
SfuI	TTCGAA	1 cut(s)	1250
SinI	GGWCC	2 cut(s)	121, 870
SlaI	CTCGAG	1 cut(s)	1394
SmiMI	CAYNNNNRTG	2 cut(s)	1274, 1887
SmlI	CTYRAG	1 cut(s)	1394
SmoI	CTYRAG	1 cut(s)	1394
SphI	GCATGC	1 cut(s)	1329
Sse9I	AATT	7 cut(s)	268, 285, 604, 1422, 1557, 1675, 1793
SsiI	CCGC	4 cut(s)	1075, 1210, 1309, 1689
SspI	AATATT	1 cut(s)	1055
SstI	GAGCTC	1 cut(s)	1949
StyD4I	CCNGG	7 cut(s)	105, 124, 844, 942, 1645, 1969, 2078
StyI	CCWWGG	3 cut(s)	673, 866, 1105
TaaI	ACNGT	4 cut(s)	1228, 1551, 1782, 2002
TaiI	ACGT	2 cut(s)	1390, 1920
TaqI	TCGA	4 cut(s)	1027, 1250, 1395, 1863
TaqII	GACCGA	1 cut(s)	236
TasI	AATT	7 cut(s)	268, 285, 604, 1422, 1557, 1675, 1793
TatI	WGTACW	2 cut(s)	1580, 1614
TfiI	GAWTC	4 cut(s)	485, 929, 1409, 1655
TscAI	CASTG	3 cut(s)	713, 1243, 1711
TseFI	GTSAC	2 cut(s)	1182, 1433
TseI	GCWGC	3 cut(s)	173, 203, 2083
Tsp45I	GTSAC	2 cut(s)	1182, 1433
TspGWI	ACGGA	1 cut(s)	1158
TspRI	CASTG	3 cut(s)	713, 1243, 1711
VneI	GTGCAC	1 cut(s)	68
VpaK11BI	GGWCC	2 cut(s)	121, 870
VspI	ATTAAT	1 cut(s)	1272
XagI	CCTNNNNNAGG	1 cut(s)	1734
XapI	RAATTY	3 cut(s)	268, 1557, 1793
XceI	RCATGY	3 cut(s)	1329, 1522, 2065
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2061
XhoI	CTCGAG	1 cut(s)	1394
XmnI	GAANNNNTTC	1 cut(s)	1654
ZrmI	AGTACT	1 cut(s)	1616
Zsp2I	ATGCAT	3 cut(s)	600, 1500, 1524

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.