Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0004829

Physical Location & Seq

Genomic Coordinates Forward (+)

20577988 .. 20591995

Gene Structure 14008 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1174900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2637 bp

ATGGAGGGCGTTGCTCTCTCTGAAGATCTCTCCGGATCCACTCAGCGTTCCGACTCGGACAGCGACCAGCGAGTCTACTTCGTTCCCTACAGTTGGTGGAGAGATGCACAGGAATCAGTTTTGGGCGATTCTGATGGGAAGAGAGGGGTTTTGTACGTGGCTTTGCAGGCTTCTTCGTATGCTGGCCCTATGAAGCTCATCAATAACATTTTCAACTCAGATCTTGTGTTCAATCTAAGGAGAGAGGATGATAGTGTACATAGTGGTGAAAATGGCGAAGTCGGTGTCTCAGGCCGAGACTACGCATTGGTGCCCGGAGAAATGTGGGTTCAGGCACTCAGATGGCATAGTGATTCTAAAGTTGCAGTGAAGGATGGTAGGAGTTTTTCAGCTGCGGAAGATGACATGACCGATGTCTATCCGCTACAACTCAGACTTTCTGTTTCACGAGAAACAAATTCATTGGGAGTAAAAGTTACAAAGAAGGATAATGCAGTTGAGTTCTTCAAGAGAGCCTGCAAAATTTTCAGTGTGGAGTCTGAGATGTTACGCATCTGGGACTTTTCGGGGCAGATAAACTTATTTTTTGCAAATGAAAGAGCCAAGTCTTCAAGAGACTTTCAACGACAGTCAGGTGAGATAATGTTGGAGTTGCAAGTTTATGGGTTGTCAGATTCCTTTAAGTGCAGGGAAGGGAAAAAAGATGAGATGGCCGCACAACATTCTACCATCACAAATTCCTGTTGTGGTGCTTCAGTTGTGATGAATGGTTCTGCGGGAATCACAAATTCTAACTTCATCCGTTCATTATGGGGAAGCTCTTGCGAAACAGGTTCTTTAGGATTGACAGGATTACAGAACCTTGGAAATACTTGTTTCATGAATAGTTCCATCCAGTGTTTAGCGCACACCCCAAAGCTTGTGGACTATTTTCTCGGAGATTATGGTAGAGAAATAAATCCAGACAATCCATTGGGCATGAATGGGGAGATTGCTTTAGCCTTTGGAGATCTGTTAAGGAAGTTATGGGTTCCTGGAGCAACTCCAGTAGCCCCAAGGCCATTTAAGTCAAAACTTTCTCGATTTGCTCCGCAGTTTAATGGATTTAATCAACATGATTCCCAAGAGCTCCTGGCTTTTTTGTTGGATGGACTTCATGAAGATCTTAATCGTGTAAAATGCAAGCCTTATGTAGAAGTCAAGGATGGTGATGGCCGACAAGATGAAGAGGTTGCTGATGAATATTGGGGAAATCATTTAGCCCGTAATGACTCTATAATTGTTGACGTGTGCCAAGGTCAATATAAATCAACATTAGTTTGCCCTGTTTGCAAAAAGGTGTCTGTTACATTTGATCCATTTATGTACCTGTCGCTACCCTTGCCTTCAATGATGATGCGGACAATGACTTTATCAGTGGTCAATACTTATGGATATTCTCAGCCGTCTCAATATACCATTACTGTGCCCAAGCATGGAAAATTAGAAGATCTCATCCAGGGTTTAAGTGTTGCATCCTCATTGGGGATTGATGAAACCTTTTTGGTGGCTGAGATATACAACAATCGCATTATACGCTATCTTGAGGAACCAACTGATTCATTATCTTTAATTAGGGATCAAGACAAGCTAGTTGCTTATCGGTCGACGAAAGATGTTGAGCAAATCCCTTTGGTTGTGTTCATGCATCAGCTGATGGAGGAGCAGTACATGGATGGGAAACTGACTTCAAGCTTTGGAATTCCTCTTGTGTCAAGACTACCCAATTCTGCTACTGGATCTGATATCTGCAACTTATACTTGAAATTACTTAAGCCATTTCACACACCCTCAAAAGATTCCCAAGAATGTTTTGATAGTTCCGAGGGCACCACTCTTGAAGAAGTCACCGGAACACAGGATTCCACACCTCCTGTTATAGGGGCTGTGAACCCCTGTAATGGAAGTGAGGCTGACTCACCCTTTGATTTTGAATTTCAGTTTTACATAACAGATGAAAAGGGAATTTCCAAGGATAGAAAGATAGTAATGAATGATATGGTAGAGAGGGAAAAAACCAAGCGGTTAAATGTCCTTGTATGTTGGCCAAAAAAGTACATCGAACAGTATGATAATCGCCTTCTTTGCTCATTACCAGAGGTTTTCAAGACTGGCCTTTTTGCAAAAAAGCCTCAAGAGTCCGTTTCTCTGTATAAGTGCCTTGAAGCGTTCTTGAAGGAAGAGCCTCTTGGACCCGAAGACATGTGGTATTGTCCTGGCTGCAAAAAGCACTGTCAAGCCAGCAAAAAGTTGGATCTATGGAGACTGCCGGAGATCTTGGTCATTCATTTAAAGCGGTTCTCATATAGCCGTTTTTTAAAGAACAAGCTGGAGACTTATGTCGATTTCCCTATTGACAATCTTGATTTGTCAACATACATTGCCCACAAGAGTGGTCAGTCATGTAATCGTTACATGCTGTATGCAGTTAGTAATCACTTTGGAAGCATGGGAGGTGGTCATTATACTGCATTTGTCCACCATGGAGGTGATCGGTGGTATGACTTTGATGATAGCCACGTTAACCCTATCAGCCAGGACAAGATAAAGTCTTCATCTGCTTATGTTCTTTTCTACAGAAGAGTTGTGGAAGTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko04137 map04137

Pfam Domains

Protein Families

UCH

Protein Analysis

879

Amino Acids

98.97

Weight (kDa)

5.5

Isoelectric Point (pI)

41.35

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ubiquitin_UBP8	PF25242	144 - 198	4.2e-31	UBP8-like, ubiquitin-like domain
UCH	PF00443	283 - 872	1.3e-73	Ubiquitin carboxyl-terminal hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0004829)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G22030 AT5G22030
fragaria_vesca	FvH4_3g26140 FvH4_3g26140 FvH4_3g26140 FvH4_3g26140 FvH4_3g26140 FvH4_3g26140 FvH4_3g26140
malus_domestica	MD03G1159600.v1.1 MD11G1174900.v1.1
prunus_persica	Prupe.6G147300_v2.0.a1 Prupe.6G147300_v2.0.a1 Prupe.6G147300_v2.0.a1 Prupe.6G147300_v2.0.a1
pyrus_communis	pycom03g11220 pycom03g11230 pycom11g14960 pycom11g14970
rosa_chinensis	RchiOBHm_Chr5g0049121
rosa_laevigata	RLG00000034590
rosa_multiflora	Rmu_sc0000530.1_g000038 Rmu_sc0000530.1_g000039
rosa_roxburghii	Rroxscaffold_1G00032700
rosa_rugosa	Rorug05G0243300 Rorug05G0243300
rosa_samantha	Rh5AG322700 Rh5BG333300 Rh5CG358200 Rh5DG344700
rosa_wichuraiana	Rw5G030460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	71
AccB1I	GGYRCC	2 cut(s)	310, 1868
AccI	GTMKAC	2 cut(s)	75, 1646
AccIII	TCCGGA	1 cut(s)	32
AciI	CCGC	8 cut(s)	395, 422, 714, 776, 1091, 1399, 2062, 2335
AclWI	GGATC	6 cut(s)	30, 43, 1349, 1626, 1786, 2301
AcoI	YGGCCR	3 cut(s)	711, 1213, 2084
AcsI	RAATTY	7 cut(s)	457, 522, 736, 787, 1740, 1973, 2004
AcuI	CTGAAG	2 cut(s)	42, 738
AfaI	GTAC	5 cut(s)	155, 258, 1367, 1709, 2096
AfiI	CCNNNNNNNGG	5 cut(s)	93, 1475, 1524, 1919, 1940
AflII	CTTAAG	1 cut(s)	1811
AflIII	ACRYGT	2 cut(s)	1287, 2241
AjiI	CACGTC	1 cut(s)	1288
AjnI	CCWGG	5 cut(s)	1033, 1131, 1497, 2254, 2574
AjuI	GAANNNNNNNTTGG	2 cut(s)	2211, 2243
AleI	CACNNNNGTG	1 cut(s)	2430
AluBI	AGCT	9 cut(s)	196, 392, 819, 919, 1129, 1630, 1693, 1734, 2368
AluI	AGCT	9 cut(s)	196, 392, 819, 919, 1129, 1630, 1693, 1734, 2368
Alw21I	GWGCWC	1 cut(s)	1131
Alw26I	GTCTC	6 cut(s)	291, 292, 609, 1452, 2296, 2366
AlwI	GGATC	6 cut(s)	30, 43, 1349, 1626, 1786, 2301
Aor13HI	TCCGGA	1 cut(s)	32
AoxI	GGCC	7 cut(s)	184, 292, 711, 1058, 1213, 2084, 2152
ApeKI	GCWGC	2 cut(s)	392, 2259
ApoI	RAATTY	7 cut(s)	457, 522, 736, 787, 1740, 1973, 2004
ArsI	GACNNNNNNTTYG	4 cut(s)	270, 302, 1946, 1978
AspLEI	GCGC	1 cut(s)	907
AspS9I	GGNCC	2 cut(s)	185, 2231
AsuC2I	CCSGG	1 cut(s)	315
AsuHPI	GGTGA	6 cut(s)	278, 647, 1220, 1879, 1950, 2540
AvaII	GGWCC	1 cut(s)	2231
BaeGI	GKGCMC	3 cut(s)	315, 1470, 1871
BalI	TGGCCA	1 cut(s)	2086
BamHI	GGATCC	1 cut(s)	35
BanI	GGYRCC	2 cut(s)	310, 1868
BanII	GRGCYC	1 cut(s)	1131
BauI	CACGAG	1 cut(s)	447
BbsI	GAAGAC	3 cut(s)	600, 2244, 2583
Bbv12I	GWGCWC	1 cut(s)	1131
BbvI	GCAGC	2 cut(s)	379, 2246
BceAI	ACGGC	2 cut(s)	1429, 2334
BciT130I	CCWGG	5 cut(s)	1035, 1133, 1499, 2256, 2576
BcnI	CCSGG	1 cut(s)	315
BcoDI	GTCTC	6 cut(s)	291, 292, 609, 1452, 2296, 2366
BfaI	CTAG	1 cut(s)	1631
BfmI	CTRYAG	2 cut(s)	88, 2614
BfrI	CTTAAG	1 cut(s)	1811
BglII	AGATCT	6 cut(s)	25, 220, 1009, 1162, 1489, 2313
BisI	GCNGC	3 cut(s)	393, 714, 2260
BlsI	GCNGC	3 cut(s)	394, 715, 2261
Bme1390I	CCNGG	6 cut(s)	315, 1035, 1133, 1499, 2256, 2576
Bme18I	GGWCC	1 cut(s)	2231
BmgBI	CACGTC	1 cut(s)	1288
BmgT120I	GGNCC	2 cut(s)	185, 2231
BmiI	GGNNCC	6 cut(s)	37, 312, 1032, 1590, 1870, 2233
BmrFI	CCNGG	6 cut(s)	315, 1035, 1133, 1499, 2256, 2576
BmsI	GCATC	5 cut(s)	94, 561, 1386, 1523, 1696
BoxI	GACNNNNGTC	2 cut(s)	413, 2378
BpiI	GAAGAC	3 cut(s)	600, 2244, 2583
BplI	GAGNNNNNCTC	2 cut(s)	1940, 1972
BpmI	CTGGAG	3 cut(s)	1029, 1056, 2390
BpuEI	CTTGAG	2 cut(s)	1604, 2157
BpuMI	CCSGG	1 cut(s)	315
BsaAI	YACGTR	1 cut(s)	157
BsaBI	GATNNNNATC	2 cut(s)	417, 1167
BsaJI	CCNNGG	7 cut(s)	862, 1055, 1294, 1498, 1863, 2010, 2521
BsaWI	WCCGGW	2 cut(s)	32, 1889
BsaXI	ACNNNNNCTCC	4 cut(s)	459, 489, 1691, 1721
Bsc4I	CCNNNNNNNGG	5 cut(s)	93, 1475, 1524, 1919, 1940
Bse1I	ACTGG	4 cut(s)	895, 1046, 1780, 2155
Bse3DI	GCAATG	1 cut(s)	2418
Bse8I	GATNNNNATC	2 cut(s)	417, 1167
BseAI	TCCGGA	1 cut(s)	32
BseBI	CCWGG	5 cut(s)	1035, 1133, 1499, 2256, 2576
BseDI	CCNNGG	7 cut(s)	862, 1055, 1294, 1498, 1863, 2010, 2521
BseGI	GGATG	9 cut(s)	253, 379, 798, 891, 1153, 1210, 1494, 1514, 1720
BseJI	GATNNNNATC	2 cut(s)	417, 1167
BseLI	CCNNNNNNNGG	5 cut(s)	93, 1475, 1524, 1919, 1940
BseMI	GCAATG	1 cut(s)	2418
BseMII	CTCAG	8 cut(s)	56, 231, 303, 352, 445, 531, 1454, 1542
BseNI	ACTGG	4 cut(s)	895, 1046, 1780, 2155
BseRI	GAGGAG	1 cut(s)	1715
BseSI	GKGCMC	3 cut(s)	315, 1470, 1871
BseXI	GCAGC	2 cut(s)	379, 2246
BsgI	GTGCAG	1 cut(s)	706
Bsh1285I	CGRYCG	1 cut(s)	1646
BshFI	GGCC	7 cut(s)	186, 294, 713, 1060, 1215, 2086, 2154
BshNI	GGYRCC	2 cut(s)	310, 1868
BsiEI	CGRYCG	1 cut(s)	1646
BsiHKAI	GWGCWC	1 cut(s)	1131
BsiSI	CCGG	4 cut(s)	33, 315, 1890, 2309
BslFI	GGGAC	1 cut(s)	572
BslI	CCNNNNNNNGG	5 cut(s)	93, 1475, 1524, 1919, 1940
BsmAI	GTCTC	6 cut(s)	291, 292, 609, 1452, 2296, 2366
BsmBI	CGTCTC	1 cut(s)	1452
BsmFI	GGGAC	1 cut(s)	572
BsnI	GGCC	7 cut(s)	186, 294, 713, 1060, 1215, 2086, 2154
Bsp1286I	GDGCHC	4 cut(s)	315, 1131, 1470, 1871
Bsp13I	TCCGGA	1 cut(s)	32
Bsp1407I	TGTACA	1 cut(s)	256
Bsp19I	CCATGG	1 cut(s)	2521
BspACI	CCGC	8 cut(s)	395, 422, 714, 776, 1091, 1399, 2062, 2335
BspANI	GGCC	7 cut(s)	186, 294, 713, 1060, 1215, 2086, 2154
BspCNI	CTCAG	8 cut(s)	55, 230, 302, 351, 444, 532, 1453, 1543
BspEI	TCCGGA	1 cut(s)	32
BspHI	TCATGA	2 cut(s)	879, 1156
BspLI	GGNNCC	6 cut(s)	37, 312, 1032, 1590, 1870, 2233
BspPI	GGATC	6 cut(s)	30, 43, 1349, 1626, 1786, 2301
BspQI	GCTCTTC	1 cut(s)	2214
BspT107I	GGYRCC	2 cut(s)	310, 1868
BspTI	CTTAAG	1 cut(s)	1811
BsrDI	GCAATG	1 cut(s)	2418
BsrGI	TGTACA	1 cut(s)	256
BsrI	ACTGG	4 cut(s)	895, 1046, 1780, 2155
BssECI	CCNNGG	7 cut(s)	862, 1055, 1294, 1498, 1863, 2010, 2521
BssSI	CACGAG	1 cut(s)	447
BssT1I	CCWWGG	5 cut(s)	862, 1055, 1294, 2010, 2521
Bst2BI	CACGAG	1 cut(s)	447
Bst2UI	CCWGG	5 cut(s)	1035, 1133, 1499, 2256, 2576
Bst4CI	ACNGT	5 cut(s)	92, 630, 1465, 2106, 2273
Bst6I	CTCTTC	4 cut(s)	134, 1221, 2214, 2614
BstAFI	CTTAAG	1 cut(s)	1811
BstAUI	TGTACA	1 cut(s)	256
BstBAI	YACGTR	1 cut(s)	157
BstC8I	GCNNGC	5 cut(s)	168, 184, 517, 1184, 2281
BstDEI	CTNAG	9 cut(s)	42, 217, 236, 289, 338, 431, 540, 1440, 1551
BstDSI	CCRYGG	1 cut(s)	2521
BstENI	CCTNNNNNAGG	1 cut(s)	1917
BstF5I	GGATG	9 cut(s)	253, 379, 798, 891, 1153, 1210, 1494, 1514, 1720
BstHHI	GCGC	1 cut(s)	907
BstMAI	GTCTC	6 cut(s)	291, 292, 609, 1452, 2296, 2366
BstMCI	CGRYCG	1 cut(s)	1646
BstMWI	GCNNNNNNNGC	5 cut(s)	167, 1329, 1381, 1575, 2124
BstNI	CCWGG	5 cut(s)	1035, 1133, 1499, 2256, 2576
BstNSI	RCATGY	2 cut(s)	2245, 2458
BstPAI	GACNNNNGTC	2 cut(s)	413, 2378
BstSCI	CCNGG	6 cut(s)	313, 1033, 1131, 1497, 2254, 2574
BstSFI	CTRYAG	2 cut(s)	88, 2614
BstSLI	GKGCMC	3 cut(s)	315, 1470, 1871
BstV1I	GCAGC	2 cut(s)	379, 2246
BstV2I	GAAGAC	3 cut(s)	600, 2244, 2583
BstX2I	RGATCY	9 cut(s)	25, 35, 220, 1009, 1162, 1489, 1778, 2293, 2313
BstXI	CCANNNNNNTGG	1 cut(s)	2432
BstYI	RGATCY	9 cut(s)	25, 35, 220, 1009, 1162, 1489, 1778, 2293, 2313
BsuRI	GGCC	7 cut(s)	186, 294, 713, 1060, 1215, 2086, 2154
BtgI	CCRYGG	1 cut(s)	2521
BtrI	CACGTC	1 cut(s)	1288
BtsCI	GGATG	9 cut(s)	253, 379, 798, 891, 1153, 1210, 1494, 1514, 1720
BtsI	GCAGTG	1 cut(s)	372
BtsIMutI	CAGTG	5 cut(s)	372, 535, 902, 1422, 2269
Cac8I	GCNNGC	5 cut(s)	168, 184, 517, 1184, 2281
CciI	TCATGA	2 cut(s)	879, 1156
CfoI	GCGC	1 cut(s)	907
Cfr13I	GGNCC	2 cut(s)	185, 2231
Csp6I	GTAC	5 cut(s)	154, 257, 1366, 1708, 2095
CspCI	CAANNNNNGTGG	2 cut(s)	903, 938
CviQI	GTAC	5 cut(s)	154, 257, 1366, 1708, 2095
DdeI	CTNAG	9 cut(s)	42, 217, 236, 289, 338, 431, 540, 1440, 1551
DraI	TTTAAA	2 cut(s)	2331, 2358
DrdI	GACNNNNNNGTC	1 cut(s)	71
DseDI	GACNNNNNNGTC	1 cut(s)	71
EaeI	YGGCCR	3 cut(s)	711, 1213, 2084
Eam1104I	CTCTTC	4 cut(s)	134, 1221, 2214, 2614
EarI	CTCTTC	4 cut(s)	134, 1221, 2214, 2614
Ecl136II	GAGCTC	1 cut(s)	1129
Eco130I	CCWWGG	5 cut(s)	862, 1055, 1294, 2010, 2521
Eco24I	GRGCYC	1 cut(s)	1131
Eco32I	GATATC	1 cut(s)	1786
Eco47I	GGWCC	1 cut(s)	2231
Eco53kI	GAGCTC	1 cut(s)	1129
Eco57I	CTGAAG	2 cut(s)	42, 738
EcoICRI	GAGCTC	1 cut(s)	1129
EcoNI	CCTNNNNNAGG	1 cut(s)	1917
EcoRI	GAATTC	1 cut(s)	1740
EcoRII	CCWGG	5 cut(s)	1033, 1131, 1497, 2254, 2574
EcoRV	GATATC	1 cut(s)	1786
EcoT14I	CCWWGG	5 cut(s)	862, 1055, 1294, 2010, 2521
EcoT22I	ATGCAT	1 cut(s)	1689
EcoT38I	GRGCYC	1 cut(s)	1131
ErhI	CCWWGG	5 cut(s)	862, 1055, 1294, 2010, 2521
Esp3I	CGTCTC	1 cut(s)	1452
FalI	AAGNNNNNCTT	2 cut(s)	2211, 2243
FaqI	GGGAC	1 cut(s)	572
FauI	CCCGC	1 cut(s)	769
FblI	GTMKAC	2 cut(s)	75, 1646
Fnu4HI	GCNGC	3 cut(s)	393, 714, 2260
FokI	GGATG	9 cut(s)	260, 386, 785, 878, 1160, 1217, 1481, 1501, 1727
FriOI	GRGCYC	1 cut(s)	1131
Fsp4HI	GCNGC	3 cut(s)	393, 714, 2260
FspBI	CTAG	1 cut(s)	1631
GlaI	GCGC	1 cut(s)	906
GluI	GCNGC	3 cut(s)	393, 714, 2260
GsuI	CTGGAG	3 cut(s)	1029, 1056, 2390
HaeIII	GGCC	7 cut(s)	186, 294, 713, 1060, 1215, 2086, 2154
HapII	CCGG	4 cut(s)	33, 315, 1890, 2309
HhaI	GCGC	1 cut(s)	907
Hin6I	GCGC	1 cut(s)	905
HinP1I	GCGC	1 cut(s)	905
HincII	GTYRAC	4 cut(s)	1285, 1647, 2412, 2563
HindII	GTYRAC	4 cut(s)	1285, 1647, 2412, 2563
HindIII	AAGCTT	2 cut(s)	917, 1732
HpaI	GTTAAC	1 cut(s)	2563
HpaII	CCGG	4 cut(s)	33, 315, 1890, 2309
HphI	GGTGA	6 cut(s)	278, 647, 1220, 1879, 1950, 2540
Hpy166II	GTNNAC	9 cut(s)	76, 257, 925, 1285, 1647, 1930, 2412, 2518, 2563
Hpy8I	GTNNAC	9 cut(s)	76, 257, 925, 1285, 1647, 1930, 2412, 2518, 2563
Hpy99I	CGWCG	1 cut(s)	1651
HpyAV	CCTTC	6 cut(s)	364, 478, 686, 1395, 2129, 2209
HpyCH4III	ACNGT	5 cut(s)	92, 630, 1465, 2106, 2273
HpyCH4IV	ACGT	3 cut(s)	156, 1287, 2559
HpyF10VI	GCNNNNNNNGC	5 cut(s)	167, 1329, 1381, 1575, 2124
HpyF3I	CTNAG	9 cut(s)	42, 217, 236, 289, 338, 431, 540, 1440, 1551
HpySE526I	ACGT	3 cut(s)	156, 1287, 2559
HspAI	GCGC	1 cut(s)	905
Kpn2I	TCCGGA	1 cut(s)	32
KspAI	GTTAAC	1 cut(s)	2563
LguI	GCTCTTC	1 cut(s)	2214
LmnI	GCTCC	4 cut(s)	1037, 1093, 1134, 1702
Lsp1109I	GCAGC	2 cut(s)	379, 2246
LweI	GCATC	5 cut(s)	94, 561, 1386, 1523, 1696
MaeI	CTAG	1 cut(s)	1631
MaeII	ACGT	3 cut(s)	156, 1287, 2559
MaeIII	GTNAC	5 cut(s)	475, 546, 1345, 1885, 2450
MflI	RGATCY	9 cut(s)	25, 35, 220, 1009, 1162, 1489, 1778, 2293, 2313
MhlI	GDGCHC	4 cut(s)	315, 1131, 1470, 1871
MlsI	TGGCCA	1 cut(s)	2086
MluNI	TGGCCA	1 cut(s)	2086
MlyI	GAGTC	6 cut(s)	47, 81, 545, 1265, 1949, 2186
MmeI	TCCRAC	4 cut(s)	75, 627, 1125, 2271
Mox20I	TGGCCA	1 cut(s)	2086
Mph1103I	ATGCAT	1 cut(s)	1689
MroI	TCCGGA	1 cut(s)	32
MscI	TGGCCA	1 cut(s)	2086
MslI	CAYNNNNRTG	5 cut(s)	264, 340, 1463, 2430, 2526
Msp20I	TGGCCA	1 cut(s)	2086
MspA1I	CMGCKG	2 cut(s)	392, 1693
MspCI	CTTAAG	1 cut(s)	1811
MspI	CCGG	4 cut(s)	33, 315, 1890, 2309
MspR9I	CCNGG	6 cut(s)	315, 1035, 1133, 1499, 2256, 2576
MvaI	CCWGG	5 cut(s)	1035, 1133, 1499, 2256, 2576
MwoI	GCNNNNNNNGC	5 cut(s)	167, 1329, 1381, 1575, 2124
NciI	CCSGG	1 cut(s)	315
NcoI	CCATGG	1 cut(s)	2521
NlaIV	GGNNCC	6 cut(s)	37, 312, 1032, 1590, 1870, 2233
NmeAIII	GCCGAG	1 cut(s)	320
NmuCI	GTSAC	1 cut(s)	1885
NsiI	ATGCAT	1 cut(s)	1689
NspI	RCATGY	2 cut(s)	2245, 2458
OliI	CACNNNNGTG	1 cut(s)	2430
PagI	TCATGA	2 cut(s)	879, 1156
PciI	ACATGT	1 cut(s)	2241
PciSI	GCTCTTC	1 cut(s)	2214
PfeI	GAWTC	9 cut(s)	113, 128, 353, 674, 780, 1118, 1598, 1838, 1901
PfoI	TCCNGGA	1 cut(s)	1033
PkrI	GCNGC	3 cut(s)	394, 715, 2261
PleI	GAGTC	6 cut(s)	47, 80, 544, 1265, 1949, 2185
PpsI	GAGTC	6 cut(s)	47, 80, 544, 1265, 1949, 2185
Ppu21I	YACGTR	1 cut(s)	157
PscI	ACATGT	1 cut(s)	2241
PshAI	GACNNNNGTC	2 cut(s)	413, 2378
Psp124BI	GAGCTC	1 cut(s)	1131
Psp6I	CCWGG	5 cut(s)	1033, 1131, 1497, 2254, 2574
PspGI	CCWGG	5 cut(s)	1033, 1131, 1497, 2254, 2574
PspN4I	GGNNCC	6 cut(s)	37, 312, 1032, 1590, 1870, 2233
PspPI	GGNCC	2 cut(s)	185, 2231
PsuI	RGATCY	9 cut(s)	25, 35, 220, 1009, 1162, 1489, 1778, 2293, 2313
PvuII	CAGCTG	2 cut(s)	392, 1693
RsaI	GTAC	5 cut(s)	155, 258, 1367, 1709, 2096
RsaNI	GTAC	5 cut(s)	154, 257, 1366, 1708, 2095
RseI	CAYNNNNRTG	5 cut(s)	264, 340, 1463, 2430, 2526
SacI	GAGCTC	1 cut(s)	1131
SalI	GTCGAC	1 cut(s)	1645
SapI	GCTCTTC	1 cut(s)	2214
SatI	GCNGC	3 cut(s)	393, 714, 2260
Sau96I	GGNCC	2 cut(s)	185, 2231
SchI	GAGTC	6 cut(s)	47, 81, 545, 1265, 1949, 2186
ScrFI	CCNGG	6 cut(s)	315, 1035, 1133, 1499, 2256, 2576
SduI	GDGCHC	4 cut(s)	315, 1131, 1470, 1871
SfaNI	GCATC	5 cut(s)	94, 561, 1386, 1523, 1696
SfcI	CTRYAG	2 cut(s)	88, 2614
SinI	GGWCC	1 cut(s)	2231
SmiMI	CAYNNNNRTG	5 cut(s)	264, 340, 1463, 2430, 2526
SmlI	CTYRAG	3 cut(s)	1583, 1811, 2172
SmoI	CTYRAG	3 cut(s)	1583, 1811, 2172
SsiI	CCGC	8 cut(s)	395, 422, 714, 776, 1091, 1399, 2062, 2335
SspI	AATATT	1 cut(s)	1244
SspMI	CTAG	1 cut(s)	1631
SstI	GAGCTC	1 cut(s)	1131
StyD4I	CCNGG	6 cut(s)	313, 1033, 1131, 1497, 2254, 2574
StyI	CCWWGG	5 cut(s)	862, 1055, 1294, 2010, 2521
TaaI	ACNGT	5 cut(s)	92, 630, 1465, 2106, 2273
TaiI	ACGT	3 cut(s)	159, 1290, 2562
TaqI	TCGA	4 cut(s)	1081, 1646, 2100, 2382
TaqII	GACCGA	2 cut(s)	425, 1632
TatI	WGTACW	3 cut(s)	256, 1707, 2094
TauI	GCSGC	1 cut(s)	716
TfiI	GAWTC	9 cut(s)	113, 128, 353, 674, 780, 1118, 1598, 1838, 1901
TscAI	CASTG	5 cut(s)	372, 535, 902, 1422, 2276
TseFI	GTSAC	1 cut(s)	1885
TseI	GCWGC	2 cut(s)	392, 2259
Tsp45I	GTSAC	1 cut(s)	1885
TspGWI	ACGGA	2 cut(s)	791, 2170
TspRI	CASTG	5 cut(s)	372, 535, 902, 1422, 2276
Vha464I	CTTAAG	1 cut(s)	1811
VpaK11BI	GGWCC	1 cut(s)	2231
XagI	CCTNNNNNAGG	1 cut(s)	1917
XapI	RAATTY	7 cut(s)	457, 522, 736, 787, 1740, 1973, 2004
XceI	RCATGY	2 cut(s)	2245, 2458
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2287
XmiI	GTMKAC	2 cut(s)	75, 1646
XspI	CTAG	1 cut(s)	1631
Zsp2I	ATGCAT	1 cut(s)	1689

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.