Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5A

Orthogroup ID

OG0004829

Physical Location & Seq

Genomic Coordinates Forward (+)

48023833 .. 48035712

Gene Structure 11880 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5AG322700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2649 bp

ATGGAGAGCGCTGGTCTCTCTGAAGATCTCTCCGATTCCACTCAGCGTCCCGACTCCGACAGCGAGCAGCGAGTTTACTTTGTTCCCTACAGTTGGTGGAGGGATGCACAAGATTCAGTTGTGGGCGATTCAGATGGGAACAAAAGGGTTTTGTGTGTGGCATCCCCGGCCGCTTCGTCTTATGGTGGCCCTATGAAGATCATAAACAACATTTTCAACTCAGATCTCGTCTTCAATCTCAGGAGAGAGGATGATGCTGTGCACAATGCTGAAAATGCCGAGGTCGGTGTATCGGGCCGGGACTATGCGTTGGTCCCCGGAGAAATGTGGGTTCAGGCGCTCAGATGGCATAGTGATCCTAAAGCTGCAGTTAAGGATGGTAGGAGTTTTTCGGCTGCAGAAGATGGCAAGACCGATGTCTATCCCCTACAACTGAGGCTTTCTGTTCCACGAGAAACAAATTCATTGGGAGTAAGAATTACCAAAAAGGATAATGCAGTTGAGTCCTTCAAAAGAGCCTGCAAAATCTTCAGTTTGGAGTCTGACTTGTTACGCATCTGGGACTTTTCCGGCCAGACTAACTTATTTTTTACAAATGACAAAACCAAGTTTCCAAGAGACTTGCAACGGCATTCAGATGAGATCCTACTGGAGTTGCAAGTTTATGGATTGTCAGATTCCATCAAGTGTAGGGAAGGGAAAAAAGATGAGATGGCCATGCAACATTCTACCATTGGAAGTTCTTGTTCTGGTTCCTCAGTTGTGATGAATGGTTCTGCTGGCAATATGCATTCCAATTTCATTCGTTCATTCTGGGGAAGTTCTTGTGAAGCTGGTTCTTTAGGGTTGACAGGATTGCAAAACCTTGGAAATACTTGTTTCATGAATAGCTCCATCCAGTGCTTAGCCCATACACCAAAGCTTGTTGACTATTTTCTTGGAGATTATGCAAGAGAAATAAATCAGGAAAACCCATTGGGCATGGATGGGGAGATTGCTTTAGCATTTGGAGATCTGTTAAGGAAGTTATGGGCTCCTGGAGCAACTCCTGTGCCTCCAAGGACCTTTAAATCAAAACTTGCTCGTTTTGCTCCTCAGTTTAATGGCTTTAATCAACATGATTCACAAGAACTCCTGGCCTTTTTGTTGGATGGACTTCATGAAGATCTTAATCGTGTGAAATGCAAGCCTTATGTAGAAGTCAAGGATGGTGATGATCGACCGGATGAAGAGGTAGCTGATGAATATTGGCGAAATCATTTAGCCCGCAATGACTCAATCATTGTTGATGTGTGCCAAGGTCAATATAAATCAACATTAGTTTGTCCTGTTTGCAAAAAGGTCTCTGTTACATTTGATCCGTTTATGTACCTGTCACTACCATTACCTTCAACAATGATGCGGACAATGACTTTAACAGTTGTCAACACTGATGGAAGTTCGAAGCCATCTGAATATACCATTAATGTGCCCAAGCATGGAAAGTTTGATGATCTCATCCGCAGTTTGAGTGTTGCATGCTCACTTGGAGTTGATGAAACCTTTTTAGTGGCCGAGATATACAACAATCGCATTATACGTTATCTCGAGGAGCCTGCTGATTCGTTATCCTTAATTAGAGATGGTGACCTGTTGGTTGCTTATCGGTTTATGAAAGATGTTGAGGAAGTACCTCTGGTTGTTTTCATGCATCAACAAATGGAGCAGGACATGCATGGGAAGTTGAGTTCAAGCTGGAAACCGTTTGGAATTCCTCTTGTATCAAGACTTTGTACTTCGGCAACTGGATCTGATATCTGCTATCAGTACTTAAACTTAGTTAAGCCATTTCAAATACCTGGAAAAGATTCGCAAGCACTTGTTGATAATTCACAGGGCACCGCTCCCATAGAGGCCACTGGAGCAGAGGGTACCGCACCTCCTGTTTCAGGTGGGGATGTGAACCTCTGTTATGAAAATGGGGCTGACTCACCGTTGGATTGTGAATTTCAGTTTTATATAACAGATGAAAAGGGTATTCAAAAGGAGAAAAAGATAGTAATGAATGAGATGGTCGAGAGGAAAATGTCAGAGCATTTATATGTTGCAGTATGTTGGCCAGAAAAATATGTTGAGCAGTATGATACTCGCGTTCTGAGCTCATTGCCGTCGGTTTTCAAGCCTGGCCTTTTTTCGAAAAAGCCTCAGGAGACTGTTTCTCTATATAAGTGCCTTGAAGCATTCTTGAAGGAGGAGCCTCTTGGGCCAGAAGACATGTGGTATTGTCCTGGCTGCAAAAAGCATTGCCAAGCCAGTAAGAAGTTGGATCTCTGGAGACTGCCTGAGATCTTGGTCATTCATTTAAAGCGGTTCTCATATAGTCGATTCTTCAAGAACAAGCTAGAGACATATGTTGACTTCCCTGTTGATAATCTGGATTTGTCAACATACATTGCTTACAGGACTGGTGAATTAAGCAATCGTTACATGCTTTATGCAGTTAGTAATCACTATGGAAGCATGGGTGGCGGTCATTATACTGCATTTGTCCATCATGGGGGCGATCGGTGGTATGACTTTGATGATAGCCATGTTAGCCCTGTGAGCCAGGACAAGATAAAGTCTGCTGCTGCGTATGTTCTTTTCTATAGGAGAGTAGTAGAAGTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko04137 map04137

Pfam Domains

Protein Families

UCH

Protein Analysis

882

Amino Acids

99.21

Weight (kDa)

5.4

Isoelectric Point (pI)

43.04

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ubiquitin_UBP8	PF25242	145 - 199	4.5e-30	UBP8-like, ubiquitin-like domain
UCH	PF00443	284 - 876	1.3e-74	Ubiquitin carboxyl-terminal hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0004829)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G22030 AT5G22030
fragaria_vesca	FvH4_3g26140 FvH4_3g26140 FvH4_3g26140 FvH4_3g26140 FvH4_3g26140 FvH4_3g26140 FvH4_3g26140
malus_domestica	MD03G1159600.v1.1 MD11G1174900.v1.1
prunus_persica	Prupe.6G147300_v2.0.a1 Prupe.6G147300_v2.0.a1 Prupe.6G147300_v2.0.a1 Prupe.6G147300_v2.0.a1
pyrus_communis	pycom03g11220 pycom03g11230 pycom11g14960 pycom11g14970
rosa_chinensis	RchiOBHm_Chr5g0049121
rosa_laevigata	RLG00000034590
rosa_multiflora	Rmu_sc0000530.1_g000038 Rmu_sc0000530.1_g000039
rosa_roxburghii	Rroxscaffold_1G00032700
rosa_rugosa	Rorug05G0243300 Rorug05G0243300
rosa_samantha	Rh5AG322700 Rh5BG333300 Rh5CG358200 Rh5DG344700
rosa_wichuraiana	Rw5G030460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	1910
AccB1I	GGYRCC	2 cut(s)	1877, 1910
AccBSI	CCGCTC	1 cut(s)	1883
AccII	CGCG	1 cut(s)	2130
AciI	CCGC	8 cut(s)	171, 1267, 1402, 1501, 1881, 1914, 2347, 2508
AclWI	GGATC	5 cut(s)	350, 637, 1352, 1795, 2313
AcoI	YGGCCR	5 cut(s)	168, 571, 714, 1551, 2096
AcsI	RAATTY	3 cut(s)	460, 1749, 1985
AcuI	CTGAAG	2 cut(s)	42, 514
AfaI	GTAC	5 cut(s)	1370, 1671, 1774, 1808, 1912
AfeI	AGCGCT	1 cut(s)	10
AfiI	CCNNNNNNNGG	4 cut(s)	93, 1478, 1928, 2536
AflIII	ACRYGT	1 cut(s)	2253
AjnI	CCWGG	6 cut(s)	1036, 1134, 1837, 2161, 2266, 2586
AloI	GAACNNNNNNTCC	2 cut(s)	1711, 1743
AluBI	AGCT	8 cut(s)	365, 833, 891, 922, 1238, 1734, 2139, 2380
AluI	AGCT	8 cut(s)	365, 833, 891, 922, 1238, 1734, 2139, 2380
Alw21I	GWGCWC	2 cut(s)	264, 2141
Alw26I	GTCTC	6 cut(s)	20, 612, 1348, 2183, 2308, 2378
Alw44I	GTGCAC	1 cut(s)	260
AlwI	GGATC	5 cut(s)	350, 637, 1352, 1795, 2313
Ama87I	CYCGRG	1 cut(s)	1586
Aor51HI	AGCGCT	1 cut(s)	10
ApaLI	GTGCAC	1 cut(s)	260
ApeKI	GCWGC	6 cut(s)	67, 365, 395, 2271, 2606, 2609
ApoI	RAATTY	3 cut(s)	460, 1749, 1985
AseI	ATTAAT	1 cut(s)	1464
Asp700I	GAANNNNTTC	1 cut(s)	1846
Asp718I	GGTACC	1 cut(s)	1910
AspLEI	GCGC	2 cut(s)	11, 340
AspS9I	GGNCC	5 cut(s)	188, 295, 313, 1062, 2243
AsuC2I	CCSGG	3 cut(s)	167, 299, 318
AsuHPI	GGTGA	4 cut(s)	1223, 1637, 1962, 2459
AsuII	TTCGAA	2 cut(s)	1442, 2174
AvaI	CYCGRG	1 cut(s)	1586
AvaII	GGWCC	2 cut(s)	313, 1062
AxyI	CCTNAGG	1 cut(s)	2184
BaeGI	GKGCMC	3 cut(s)	264, 1473, 1880
BaeI	ACNNNNGTAYC	2 cut(s)	2115, 2148
BalI	TGGCCA	2 cut(s)	716, 2098
BanI	GGYRCC	2 cut(s)	1877, 1910
BanII	GRGCYC	2 cut(s)	1036, 2141
BauI	CACGAG	1 cut(s)	450
BbsI	GAAGAC	2 cut(s)	223, 2256
Bbv12I	GWGCWC	2 cut(s)	264, 2141
BbvI	GCAGC	6 cut(s)	79, 352, 382, 2258, 2593, 2596
BceAI	ACGGC	2 cut(s)	644, 2131
BcgI	CGANNNNNNTGC	2 cut(s)	1577, 1611
BciT130I	CCWGG	6 cut(s)	1038, 1136, 1839, 2163, 2268, 2588
BcnI	CCSGG	3 cut(s)	167, 299, 318
BcoDI	GTCTC	6 cut(s)	20, 612, 1348, 2183, 2308, 2378
BfaI	CTAG	1 cut(s)	2381
BfmI	CTRYAG	4 cut(s)	88, 366, 396, 2626
BfoI	RGCGCY	2 cut(s)	12, 341
BglI	GCCNNNNNGGC	1 cut(s)	2242
BglII	AGATCT	5 cut(s)	25, 223, 1012, 1165, 2325
BisI	GCNGC	7 cut(s)	68, 171, 366, 396, 2272, 2607, 2610
BlpI	GCTNAGC	1 cut(s)	904
BlsI	GCNGC	7 cut(s)	69, 172, 367, 397, 2273, 2608, 2611
BmcAI	AGTACT	1 cut(s)	1808
Bme1390I	CCNGG	9 cut(s)	167, 299, 318, 1038, 1136, 1839, 2163, 2268, 2588
Bme18I	GGWCC	2 cut(s)	313, 1062
BmeT110I	CYCGRG	1 cut(s)	1586
BmgT120I	GGNCC	5 cut(s)	188, 295, 313, 1062, 2243
BmiI	GGNNCC	7 cut(s)	315, 754, 1035, 1593, 1879, 1912, 2235
BmrFI	CCNGG	9 cut(s)	167, 299, 318, 1038, 1136, 1839, 2163, 2268, 2588
BmsI	GCATC	6 cut(s)	94, 170, 244, 564, 1389, 1699
BoxI	GACNNNNGTC	1 cut(s)	416
BpiI	GAAGAC	2 cut(s)	223, 2256
BpmI	CTGGAG	4 cut(s)	671, 1059, 1920, 2332
Bpu1102I	GCTNAGC	1 cut(s)	904
Bpu14I	TTCGAA	2 cut(s)	1442, 2174
BpuMI	CCSGG	3 cut(s)	167, 299, 318
BsaBI	GATNNNNATC	2 cut(s)	420, 1170
BsaI	GGTCTC	2 cut(s)	20, 1348
BsaJI	CCNNGG	6 cut(s)	165, 279, 316, 865, 1058, 1297
BsaWI	WCCGGW	1 cut(s)	1222
BsaXI	ACNNNNNCTCC	4 cut(s)	1116, 1146, 1903, 1933
Bsc4I	CCNNNNNNNGG	4 cut(s)	93, 1478, 1928, 2536
Bse1I	ACTGG	6 cut(s)	654, 898, 1789, 1903, 2292, 2449
Bse21I	CCTNAGG	1 cut(s)	2184
Bse3DI	GCAATG	4 cut(s)	1276, 2141, 2281, 2430
Bse8I	GATNNNNATC	2 cut(s)	420, 1170
BseBI	CCWGG	6 cut(s)	1038, 1136, 1839, 2163, 2268, 2588
BseDI	CCNNGG	6 cut(s)	165, 279, 316, 865, 1058, 1297
BseJI	GATNNNNATC	2 cut(s)	420, 1170
BseLI	CCNNNNNNNGG	4 cut(s)	93, 1478, 1928, 2536
BseMI	GCAATG	4 cut(s)	1276, 2141, 2281, 2430
BseNI	ACTGG	6 cut(s)	654, 898, 1789, 1903, 2292, 2449
BseRI	GAGGAG	3 cut(s)	1083, 1604, 2246
BseSI	GKGCMC	3 cut(s)	264, 1473, 1880
BseX3I	CGGCCG	1 cut(s)	168
BseXI	GCAGC	6 cut(s)	79, 352, 382, 2258, 2593, 2596
Bsh1236I	CGCG	1 cut(s)	2130
Bsh1285I	CGRYCG	3 cut(s)	171, 1223, 2545
BshNI	GGYRCC	2 cut(s)	1877, 1910
BsiEI	CGRYCG	3 cut(s)	171, 1223, 2545
BsiHKAI	GWGCWC	2 cut(s)	264, 2141
BsiHKCI	CYCGRG	1 cut(s)	1586
BsiSI	CCGG	5 cut(s)	167, 298, 318, 570, 1223
BslFI	GGGAC	4 cut(s)	33, 299, 314, 575
BslI	CCNNNNNNNGG	4 cut(s)	93, 1478, 1928, 2536
BsmAI	GTCTC	6 cut(s)	20, 612, 1348, 2183, 2308, 2378
BsmFI	GGGAC	4 cut(s)	33, 299, 314, 575
BsmI	GAATGC	3 cut(s)	631, 790, 2219
Bso31I	GGTCTC	2 cut(s)	20, 1348
BsoBI	CYCGRG	1 cut(s)	1586
Bsp119I	TTCGAA	2 cut(s)	1442, 2174
Bsp1286I	GDGCHC	5 cut(s)	264, 1036, 1473, 1880, 2141
Bsp1720I	GCTNAGC	1 cut(s)	904
BspACI	CCGC	8 cut(s)	171, 1267, 1402, 1501, 1881, 1914, 2347, 2508
BspFNI	CGCG	1 cut(s)	2130
BspHI	TCATGA	2 cut(s)	882, 1159
BspLI	GGNNCC	7 cut(s)	315, 754, 1035, 1593, 1879, 1912, 2235
BspMAI	CTGCAG	2 cut(s)	370, 400
BspPI	GGATC	5 cut(s)	350, 637, 1352, 1795, 2313
BspT104I	TTCGAA	2 cut(s)	1442, 2174
BspT107I	GGYRCC	2 cut(s)	1877, 1910
BspTNI	GGTCTC	2 cut(s)	20, 1348
BsrBI	CCGCTC	1 cut(s)	1883
BsrDI	GCAATG	4 cut(s)	1276, 2141, 2281, 2430
BsrI	ACTGG	6 cut(s)	654, 898, 1789, 1903, 2292, 2449
BssECI	CCNNGG	6 cut(s)	165, 279, 316, 865, 1058, 1297
BssSI	CACGAG	1 cut(s)	450
BssT1I	CCWWGG	3 cut(s)	865, 1058, 1297
Bst2BI	CACGAG	1 cut(s)	450
Bst2UI	CCWGG	6 cut(s)	1038, 1136, 1839, 2163, 2268, 2588
Bst4CI	ACNGT	5 cut(s)	92, 1420, 1743, 1974, 2194
Bst6I	CTCTTC	1 cut(s)	1224
BstAPI	GCANNNNNTGC	1 cut(s)	1711
BstBI	TTCGAA	2 cut(s)	1442, 2174
BstC8I	GCNNGC	8 cut(s)	65, 520, 781, 1187, 1267, 1519, 1596, 1854
BstEII	GGTNACC	1 cut(s)	1625
BstENI	CCTNNNNNAGG	1 cut(s)	1926
BstFNI	CGCG	1 cut(s)	2130
BstH2I	RGCGCY	2 cut(s)	12, 341
BstHHI	GCGC	2 cut(s)	11, 340
BstMAI	GTCTC	6 cut(s)	20, 612, 1348, 2183, 2308, 2378
BstMCI	CGRYCG	3 cut(s)	171, 1223, 2545
BstNI	CCWGG	6 cut(s)	1038, 1136, 1839, 2163, 2268, 2588
BstNSI	RCATGY	4 cut(s)	1521, 1714, 2257, 2470
BstPAI	GACNNNNGTC	1 cut(s)	416
BstPI	GGTNACC	1 cut(s)	1625
BstSCI	CCNGG	9 cut(s)	165, 297, 316, 1036, 1134, 1837, 2161, 2266, 2586
BstSFI	CTRYAG	4 cut(s)	88, 366, 396, 2626
BstSLI	GKGCMC	3 cut(s)	264, 1473, 1880
BstUI	CGCG	1 cut(s)	2130
BstV1I	GCAGC	6 cut(s)	79, 352, 382, 2258, 2593, 2596
BstV2I	GAAGAC	2 cut(s)	223, 2256
BstX2I	RGATCY	8 cut(s)	25, 223, 642, 1012, 1165, 1787, 2305, 2325
BstYI	RGATCY	8 cut(s)	25, 223, 642, 1012, 1165, 1787, 2305, 2325
BstZI	CGGCCG	1 cut(s)	168
Bsu36I	CCTNAGG	1 cut(s)	2184
BtsIMutI	CAGTG	3 cut(s)	905, 1428, 1896
Cac8I	GCNNGC	8 cut(s)	65, 520, 781, 1187, 1267, 1519, 1596, 1854
CciI	TCATGA	2 cut(s)	882, 1159
CfoI	GCGC	2 cut(s)	11, 340
Cfr13I	GGNCC	5 cut(s)	188, 295, 313, 1062, 2243
CseI	GACGC	1 cut(s)	35
Csp6I	GTAC	5 cut(s)	1369, 1670, 1773, 1807, 1911
CviQI	GTAC	5 cut(s)	1369, 1670, 1773, 1807, 1911
DraI	TTTAAA	2 cut(s)	1069, 2343
EaeI	YGGCCR	5 cut(s)	168, 571, 714, 1551, 2096
EagI	CGGCCG	1 cut(s)	168
Eam1104I	CTCTTC	1 cut(s)	1224
EarI	CTCTTC	1 cut(s)	1224
Ecl136II	GAGCTC	1 cut(s)	2139
EclXI	CGGCCG	1 cut(s)	168
Eco130I	CCWWGG	3 cut(s)	865, 1058, 1297
Eco24I	GRGCYC	2 cut(s)	1036, 2141
Eco31I	GGTCTC	2 cut(s)	20, 1348
Eco32I	GATATC	1 cut(s)	1795
Eco47I	GGWCC	2 cut(s)	313, 1062
Eco47III	AGCGCT	1 cut(s)	10
Eco52I	CGGCCG	1 cut(s)	168
Eco53kI	GAGCTC	1 cut(s)	2139
Eco57I	CTGAAG	2 cut(s)	42, 514
Eco81I	CCTNAGG	1 cut(s)	2184
Eco88I	CYCGRG	1 cut(s)	1586
Eco91I	GGTNACC	1 cut(s)	1625
EcoICRI	GAGCTC	1 cut(s)	2139
EcoNI	CCTNNNNNAGG	1 cut(s)	1926
EcoO109I	RGGNCCY	1 cut(s)	1062
EcoO65I	GGTNACC	1 cut(s)	1625
EcoRI	GAATTC	1 cut(s)	1749
EcoRII	CCWGG	6 cut(s)	1036, 1134, 1837, 2161, 2266, 2586
EcoRV	GATATC	1 cut(s)	1795
EcoT14I	CCWWGG	3 cut(s)	865, 1058, 1297
EcoT22I	ATGCAT	3 cut(s)	792, 1692, 1716
EcoT38I	GRGCYC	2 cut(s)	1036, 2141
ErhI	CCWWGG	3 cut(s)	865, 1058, 1297
FaqI	GGGAC	4 cut(s)	33, 299, 314, 575
FauI	CCCGC	1 cut(s)	1274
FauNDI	CATATG	1 cut(s)	2389
Fnu4HI	GCNGC	7 cut(s)	68, 171, 366, 396, 2272, 2607, 2610
FriOI	GRGCYC	2 cut(s)	1036, 2141
Fsp4HI	GCNGC	7 cut(s)	68, 171, 366, 396, 2272, 2607, 2610
FspBI	CTAG	1 cut(s)	2381
GlaI	GCGC	2 cut(s)	10, 339
GluI	GCNGC	7 cut(s)	68, 171, 366, 396, 2272, 2607, 2610
GsuI	CTGGAG	4 cut(s)	671, 1059, 1920, 2332
HaeII	RGCGCY	2 cut(s)	12, 341
HapII	CCGG	5 cut(s)	167, 298, 318, 570, 1223
HgaI	GACGC	1 cut(s)	35
HhaI	GCGC	2 cut(s)	11, 340
Hin6I	GCGC	2 cut(s)	9, 338
HinP1I	GCGC	2 cut(s)	9, 338
HincII	GTYRAC	5 cut(s)	849, 928, 1426, 2395, 2424
HindII	GTYRAC	5 cut(s)	849, 928, 1426, 2395, 2424
HindIII	AAGCTT	1 cut(s)	920
HpaII	CCGG	5 cut(s)	167, 298, 318, 570, 1223
HphI	GGTGA	4 cut(s)	1223, 1637, 1962, 2459
Hpy166II	GTNNAC	8 cut(s)	76, 262, 849, 928, 1426, 1942, 2395, 2424
Hpy8I	GTNNAC	8 cut(s)	76, 262, 849, 928, 1426, 1942, 2395, 2424
Hpy99I	CGWCG	1 cut(s)	2152
HpyAV	CCTTC	4 cut(s)	517, 689, 1398, 2221
HpyCH4III	ACNGT	5 cut(s)	92, 1420, 1743, 1974, 2194
HpyCH4IV	ACGT	1 cut(s)	1579
HpySE526I	ACGT	1 cut(s)	1579
HspAI	GCGC	2 cut(s)	9, 338
KpnI	GGTACC	1 cut(s)	1914
LmnI	GCTCC	9 cut(s)	896, 1039, 1040, 1096, 1591, 1702, 1888, 1901, 2233
Lsp1109I	GCAGC	6 cut(s)	79, 352, 382, 2258, 2593, 2596
LweI	GCATC	6 cut(s)	94, 170, 244, 564, 1389, 1699
MaeI	CTAG	1 cut(s)	2381
MaeII	ACGT	1 cut(s)	1579
MaeIII	GTNAC	5 cut(s)	549, 1348, 1374, 1625, 2462
MbiI	CCGCTC	1 cut(s)	1883
MboII	GAAGA	9 cut(s)	35, 208, 223, 413, 520, 1175, 1241, 2261, 2359
MflI	RGATCY	8 cut(s)	25, 223, 642, 1012, 1165, 1787, 2305, 2325
MhlI	GDGCHC	5 cut(s)	264, 1036, 1473, 1880, 2141
MlsI	TGGCCA	2 cut(s)	716, 2098
MluCI	AATT	8 cut(s)	460, 477, 796, 1614, 1749, 1867, 1985, 2450
MluNI	TGGCCA	2 cut(s)	716, 2098
MlyI	GAGTC	5 cut(s)	47, 512, 548, 1268, 1961
MmeI	TCCRAC	4 cut(s)	81, 1128, 1956, 2283
Mox20I	TGGCCA	2 cut(s)	716, 2098
Mph1103I	ATGCAT	3 cut(s)	792, 1692, 1716
MroXI	GAANNNNTTC	1 cut(s)	1846
MscI	TGGCCA	2 cut(s)	716, 2098
MslI	CAYNNNNRTG	3 cut(s)	636, 1466, 2079
Msp20I	TGGCCA	2 cut(s)	716, 2098
MspI	CCGG	5 cut(s)	167, 298, 318, 570, 1223
MspR9I	CCNGG	9 cut(s)	167, 299, 318, 1038, 1136, 1839, 2163, 2268, 2588
Mva1269I	GAATGC	3 cut(s)	631, 790, 2219
MvaI	CCWGG	6 cut(s)	1038, 1136, 1839, 2163, 2268, 2588
MvnI	CGCG	1 cut(s)	2130
NciI	CCSGG	3 cut(s)	167, 299, 318
NdeI	CATATG	1 cut(s)	2389
NlaIV	GGNNCC	7 cut(s)	315, 754, 1035, 1593, 1879, 1912, 2235
NmeAIII	GCCGAG	2 cut(s)	304, 1579
NmuCI	GTSAC	2 cut(s)	1374, 1625
NsiI	ATGCAT	3 cut(s)	792, 1692, 1716
NspI	RCATGY	4 cut(s)	1521, 1714, 2257, 2470
NspV	TTCGAA	2 cut(s)	1442, 2174
PaeI	GCATGC	1 cut(s)	1521
PaeR7I	CTCGAG	1 cut(s)	1586
PagI	TCATGA	2 cut(s)	882, 1159
PciI	ACATGT	1 cut(s)	2253
PcsI	WCGNNNNNNNCGW	1 cut(s)	1576
PctI	GAATGC	3 cut(s)	631, 790, 2219
PdmI	GAANNNNTTC	1 cut(s)	1846
PfeI	GAWTC	8 cut(s)	35, 113, 128, 677, 1121, 1601, 1847, 2364
PfoI	TCCNGGA	1 cut(s)	1036
PkrI	GCNGC	7 cut(s)	69, 172, 367, 397, 2273, 2608, 2611
Ple19I	CGATCG	1 cut(s)	2545
PleI	GAGTC	5 cut(s)	47, 511, 547, 1268, 1961
PpsI	GAGTC	5 cut(s)	47, 511, 547, 1268, 1961
PpuMI	RGGWCCY	1 cut(s)	1062
PscI	ACATGT	1 cut(s)	2253
PshAI	GACNNNNGTC	1 cut(s)	416
PshBI	ATTAAT	1 cut(s)	1464
Psp124BI	GAGCTC	1 cut(s)	2141
Psp5II	RGGWCCY	1 cut(s)	1062
Psp6I	CCWGG	6 cut(s)	1036, 1134, 1837, 2161, 2266, 2586
PspEI	GGTNACC	1 cut(s)	1625
PspGI	CCWGG	6 cut(s)	1036, 1134, 1837, 2161, 2266, 2586
PspN4I	GGNNCC	7 cut(s)	315, 754, 1035, 1593, 1879, 1912, 2235
PspPI	GGNCC	5 cut(s)	188, 295, 313, 1062, 2243
PspPPI	RGGWCCY	1 cut(s)	1062
PstI	CTGCAG	2 cut(s)	370, 400
PsuI	RGATCY	8 cut(s)	25, 223, 642, 1012, 1165, 1787, 2305, 2325
PvuI	CGATCG	1 cut(s)	2545
RsaI	GTAC	5 cut(s)	1370, 1671, 1774, 1808, 1912
RsaNI	GTAC	5 cut(s)	1369, 1670, 1773, 1807, 1911
RseI	CAYNNNNRTG	3 cut(s)	636, 1466, 2079
SacI	GAGCTC	1 cut(s)	2141
SatI	GCNGC	7 cut(s)	68, 171, 366, 396, 2272, 2607, 2610
Sau96I	GGNCC	5 cut(s)	188, 295, 313, 1062, 2243
ScaI	AGTACT	1 cut(s)	1808
SchI	GAGTC	5 cut(s)	47, 512, 548, 1268, 1961
ScrFI	CCNGG	9 cut(s)	167, 299, 318, 1038, 1136, 1839, 2163, 2268, 2588
SduI	GDGCHC	5 cut(s)	264, 1036, 1473, 1880, 2141
SfaNI	GCATC	6 cut(s)	94, 170, 244, 564, 1389, 1699
SfcI	CTRYAG	4 cut(s)	88, 366, 396, 2626
Sfr274I	CTCGAG	1 cut(s)	1586
SfuI	TTCGAA	2 cut(s)	1442, 2174
SinI	GGWCC	2 cut(s)	313, 1062
SlaI	CTCGAG	1 cut(s)	1586
SmiMI	CAYNNNNRTG	3 cut(s)	636, 1466, 2079
SmlI	CTYRAG	1 cut(s)	1586
SmoI	CTYRAG	1 cut(s)	1586
SphI	GCATGC	1 cut(s)	1521
Sse9I	AATT	8 cut(s)	460, 477, 796, 1614, 1749, 1867, 1985, 2450
SsiI	CCGC	8 cut(s)	171, 1267, 1402, 1501, 1881, 1914, 2347, 2508
SspI	AATATT	1 cut(s)	1247
SspMI	CTAG	1 cut(s)	2381
SstI	GAGCTC	1 cut(s)	2141
StyD4I	CCNGG	9 cut(s)	165, 297, 316, 1036, 1134, 1837, 2161, 2266, 2586
StyI	CCWWGG	3 cut(s)	865, 1058, 1297
TaaI	ACNGT	5 cut(s)	92, 1420, 1743, 1974, 2194
TaiI	ACGT	1 cut(s)	1582
TaqI	TCGA	6 cut(s)	1219, 1442, 1587, 2055, 2174, 2362
TaqII	GACCGA	1 cut(s)	428
TasI	AATT	8 cut(s)	460, 477, 796, 1614, 1749, 1867, 1985, 2450
TatI	WGTACW	2 cut(s)	1772, 1806
TauI	GCSGC	1 cut(s)	173
TfiI	GAWTC	8 cut(s)	35, 113, 128, 677, 1121, 1601, 1847, 2364
TscAI	CASTG	3 cut(s)	905, 1435, 1903
TseFI	GTSAC	2 cut(s)	1374, 1625
TseI	GCWGC	6 cut(s)	67, 365, 395, 2271, 2606, 2609
Tsp45I	GTSAC	2 cut(s)	1374, 1625
TspGWI	ACGGA	1 cut(s)	1350
TspRI	CASTG	3 cut(s)	905, 1435, 1903
VneI	GTGCAC	1 cut(s)	260
VpaK11BI	GGWCC	2 cut(s)	313, 1062
VspI	ATTAAT	1 cut(s)	1464
XagI	CCTNNNNNAGG	1 cut(s)	1926
XapI	RAATTY	3 cut(s)	460, 1749, 1985
XceI	RCATGY	4 cut(s)	1521, 1714, 2257, 2470
XcmI	CCANNNNNNNNNTGG	2 cut(s)	2253, 2299
XhoI	CTCGAG	1 cut(s)	1586
XmnI	GAANNNNTTC	1 cut(s)	1846
XspI	CTAG	1 cut(s)	2381
ZrmI	AGTACT	1 cut(s)	1808
Zsp2I	ATGCAT	3 cut(s)	792, 1692, 1716

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.