FvH4_3g28920

Proteasome-associated protein ECM29 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0006358
Physical Location & Seq
Forward (+)
21987083 .. 22012921
25839 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g28920.t2

Sequence Viewer

Length: 5439 bp
ATGGCGGAAACATCGTCTTCCAAATCGGACGAAGAGAGAGAAGAGATGCTGGACCGGCTGCTCACCCGCCTCGCTCTTTCCGACGACTCCAAACTCCAACCTCTACTCTCCAAGCTCCTCCCTTACACTATCTCCTCCCTCTCCTCTCACTCCTCCGCCGTCCGCAACAAGGTTATGGAAATACTGAGTCATGTGAACAAGCGAGTGAAGCATCAACCGGAGATTGGTTTGCCCTTGTCTGAACTATGGACTATATTCTCCGGAGCTGATTCTGCTCCGATGGTCAGGAATTTTTGCATTCTCTACATGGAAATGGCAATGGATCGTGCCGATACAAAGGAGAAAGAAAATTTGTCACCCATGCTTCTGGTGGGAGTCTCAAAGCTTTCTAATCAGCACCAAGAAATTATTCTCAGACTTGTTGTGAAGGTCATTGGTGAATGTCACCCAAATGGAATAGATGGAGAAATTGCTGCAAAGTATGGACTTATAACTGATTCTCAAGACAGGAGCATTTTTATCGAATTTTGCCTTCATACAATCTTATATCAGCAGTCATCTCAAAGAGAATGCCCACCAGGACTTTCAATTGCGCAAGCCAACCGGGTAACAGCTAAACAATCATTGAACAGTGATATACTTCTAAATAGAAAGTTAGGGATCTTGAATGTTATTGAGGCCATGGAGCTGGCCCCTGAGCTCGTTTATCCTTTGTACCTGACTGCCTCCATAGATTGTCAAGAGCCTGTCGTTAAGAGAGGAGAGGAGCTTGTAAAGAAGAGGGCTGCTGGTGCTAATTTTGAAGATACAGTCCTCATAAGCAGACTTTTTTTGTTATTTAATGGTACTGCTACATCTCACAATGTTGGTTCTGAATCTAGAGTCACTCCAGCAAGTCCGGCTTTGAAAGGAAAACTGATGTCTATTTTCTGCCGGTCTATCACAGCAGCTAACAGTTTCCCTTCAACTTTACAGTGCATTTTTGGATGCATATATGGGAGTGATACCACATCAAGGTTGAAGCAGTTGGGAATGGAATTCACTGTCTGGGTATTTAAACATTCCAAAATCGATCAACTTAAACTAATGGGCCCTGTCATACTGAGTGGAATTCTGAAGTCACTTGACACTGCTTCAAGTTCAGAATCAGATGCTACTCATAGAGACTCCAGAACTTTCGCTTATCAAGCTATTGGATTGCTCGCACAGCGTATGCCTCAGCTTTTCAGAGATTCGACTGACATGGCTGTGCGTCTCTTTGATGCATTGAAGGTAGAGACCCAATATTTTCGCCTTAGTATTCAAGAAGCAACAAATTCTCTTGCCACTGCTTATAAGGGAGCACCCTCAACAGTGCTAAAGGATCTGGAGACTCTTTTGCTCAAGTATACTCAGGAGGAACAAAGTGAAGTGAGATTCTGTGCTATAAGATGGGCAACTTCTTTATTTGAGTTGCAACATTGTCCAAGTCGGTATATCTGTATGCTTGGGGCTGCTGATATTAAGCTGGATATAAGGGAGATTGCATTGGAAGGCCTTTTTCCTGTGGAAGATGATGGATCAAGTATGAGCAAGATAAAAGAGCTACATTACCCCAAACTTGGAGACATGTTAGATTATATTCTTTCACAGCAGCCAAACTTGTCAGAATCAGCTGAGACCAGGGATCAGAAGCTTCAGTTTCCCTCAAGAACGTATCTAGTTATTATTGAATTTTTGTTGAAGTGCTTCGAGTCAGAATTGGAGCATAATACTTCAATTAAAGGATCATCTCAGTTTCAATGGTCTGTAGAAGCAATGTGCTTGCTCCTGGAACATGCTATGGCATATGAAGGATCTGTTGAGTTGTACGCCAAGGCTTCCAATGCATTGATTGCAATTGGATCTCGTATACCAGAGCTGGTAGCTTCACGTTATGCAAAAAAAGTTCCATGGCTAAAACAATTATTGAGTCATATTGACTTAGATACCCGTGAAGCGGCTGCACGTTTGCTTGGGATTGCTTCCTCCGTTCTTCCTATAGATGCTTCATGTGCTCTGATATCTGAAATAATTGCCTCAGTAAGGGGAATAAATAAGTTAAGATTTGAGGTTCAGCATGGAGCATTATGTGCATTAGGTTATGTTACTGCAAATTGTATGTCTAGAAAACCAGCTATCCCAGAGGAATTATTTCAAATTACATTAAAGTTACTTGTCGATGTTGTTAATTCTGAGACTGCAACACTAGCATCAGTAGCGGTCCAAGCACTAGGTCATATTGGACTGGTTGTTGCATTGCCTTCTCTTATTGTTGAGTCTAGTTCAGTTGATATTCTGGTGGTTTTACAAGAAAGATTGACAAAGCTAATTAAGGGTGATGACTCCAAAGCAATTCAGAAGATTATTATATCCATTGGCCACATCTGCATCAATGAAACATCATCTGCATGTCTTAATATTGCCCTGGAGTTAATATTCAGTCTTTCCCGATCTAAGGTTGAGGATATCCTCTTTGCTGCTGGCGAGGCACTATCGTTTTTGTGGGGTGGTGTTCCGGTGACTGCGGATTTGATTCTCAAAACTAATTATTCACTCTCCATGGCTTCTAAGTTCCTCATGGGAGACCCGAGTTTATCTCTCTCAACACATAGCCCTATAGAAATGAATGAAGCTAATAAAGATCGTGATGCCATGGTTCGAGAAGCAATTACTAAAAAGCTTTTTGATGAACTTCTATACAGTACAAGAAAGGAAGATCGCTGTGCTGGAACTGTATGGCTATTATCAATTACCATGTATTGTGGCCATCAACCAGCTATCCAGAAAATGCTTCCAGAAATTCAGGAGGCTTTTTCACATCTTCTTGGGGAGCAGAATGAACTCACACAGGAGCTTGCCTCCCAAGGGATGAGCGTTGTGTATGAAATTGGTGATGCGTCGATGAAAGGAAACTTGGTAAATGCGCTTGTAAACACTCTGACAGGTTCAGGGAAGAAAAAAAGAGCTATAAAGCTTGCTGAAGACTCAGAAGTGTTTCAAGAGGGAGTTATTGGTGAAGGTCTTAGTGGAGGGAAACTCAGTACATACAAGGAGCTGTGCAACGTAGCGAATGAGATGGGACAACCAGATCTGATTTATAAGTTTATGGATCTGGCTAATTATCAAACTTCTCTGAATTCAAAAAGGGGTGCTGCCTTTGGTTTTTCAAAGATAGCAAAGCAAGCAGGAGATGCTCTGAAGCCACGCTTGCGGTCCTTAATTCCAAGGCTTGTTCGTTATCAGTATGATCCTGACAAAAATGTGCAGGATGCCATGTCACACATATGGAAGTCACTGGTGGAAGATTCAAAGAAGACCATTGATGAACACTTGGACCTCATTATTGATGACTTGTTAATACAGTGTGGATCTAGGCTTTGGCGCACACGAGAGGCATCCTGTCTTGCCCTTGCAGATATCATCCAGGGCCGCAAGTTTGATCAGGTTGGGAAGCATTTAAGGAAGTTATGGCCAGCAGCATTCCGTGCCATGGATGATATTAAAGAGACTGTTCGAAATTCTGGAGACAAGTTATGCCGTACTTTAACTTCATTGACAGTGAGGCTTTCTGATGTCACCCTAACTGATGTATCAGATGCAAGCCAATCAATGGATCTTGTTCTGCCTTTTTTGCTTACAGAAGGCATACTAAGTAAAGTTGATAGTATTCGGAAGGCATCTATAGAGGTTGTTATGAAGCTTGCAAAGGGGGCTGGCATTGCAATCCGTTCACATTTATCTGATCTAGTTTGCTGCATGCTGGAAAGTTTGTCGAGCCTTGAGGACCAAGGGCTTAATTACGTCGAGTTGCATGCAGCAAATGCTGGGATACAGACAGAGAAGCTTGAGAGTTTGAGAATTTCGATTGCAAAAGGCTCTCCGATGTGGGAAACTCTTGATCTCTGCATAAAAGTTGTGGATGCAGGATCATTAGATCAATTGGTTCCCCGTCTTGGTCAACTGGTTCGATCTGGGGTGGGTCTCAATACAAGGGTTGGTGTCGCCAGCTTTATTACCTTACTAGTTCAAGAAGTTGGTGTTGAAATTAAACCATATACGAGCAAGCTACTGAGGCTGTTGTTTCCAGTTGTGAAGGAGGAAAAAAGTGCTGCATCAAAACGAGCCTTTGCTGATGCATGTGCAGTACTTTTAAAACATACAGTAGCCTCGCAGGCTGAAAAGTTGATCGACGACACTGCAGCTTTGCATGCTGGTGACCGAAATGCTCAAGTTGCCTGTGCAGTTTTATTGAAAAGCTATTCATCCAAGGCGTCAGACATTTTGGATGGATATCTTGCAGCTATACTTCCAGTAATTTTTATTTCAAGGTTTGACGATGACAAGTATGTTTCTGGCCTGTTTGAAGAGTTGTGGGAAGAGCATACAAGTAGTGAACGTGTAGCCCTCCAGTTATACTTGGCAGAAATTGTTTCTCTTATTTGTGAGAGTATTGCAACATCCTCTTGGGCAAGTAAAAAGAAGGCAGCCCAGGCAATTAACAAGCTCAGTGAAGTTTTGGGTGAATCTTTGGCTTCTTACTATAATGTCTTGCTCCAGTCTCTTATGAAAGAGATTCCTGGTCGGTTATGGGAGGGAAAGGAAGCTCTATTATATTCGATTGCTGCACTTTGTGTGTCATGCCACAAAGCAATTTCAACTGATGATTCTCACACTCTGAATGAAGTTTTGAGGGTGGTATCTTCAGCATGCACAAAGAAGGCAAAGAAATATCGTGAGGCAGCTCTATCCTGTCTGGAACAGGTTGTAAAGGCCTTTGGTAATGAGGAGTTCTTTAACGAGGCATTTCTAATGTTGTATGATATGTGTAATGCTTCCGCTCTCGGTGCTTCTGGGAAAGCAACTTTGGCTGGAAGTGGTGCTAAAGCAGAAGAGGATCACATTGAGCAGGTTCATGTTCCACATGAGAAAATTTTGGACTGCATGACCGCTTGCATTAATGTGGCTAAGGTAAAAGATATTCATGAACAACAGAAGAATTTAATGCAGGTCTTGACTACTGCTCTGTCACCTGGATTTCCATGGACAGTCAAAATATCTGCCTTTTCATTGATAAAGGAACTTGGTTCAAGTGTCCACAAGGTTGTGGCTGACCCTCAACAAAGTAATGATCATGCTAACATTATTTTGTTAGTTCAGGAGTTGTTTCACTCAGTGGCACCTCTAGTTGTCGAATGCATAAGCACAGTTAAAGTTGGGCAGGTTCATGTTGCTGCTTCAGAGTGCCTTTTAGGGATCATGAAGCTTTACAGAGACCTTCGGTCAATCAACTGTACTAATGTACAATTTCAGGGTACACTCTTGCATCTGTATGAAGTGGAGAAAAATGGCGAAGCAAAGTCCTTGTTGAAAAAATGTGTTGATACTCTTGAAAACATAACTAGAATGTCCGAGAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ecm29

Protein Analysis

1813

Amino Acids

199.59

Weight (kDa)

6.04

Isoelectric Point (pI)

42.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_ECM29 PF23702 100 - 229 5.9e-12 ECM29, ARM-like repeats
HEAT_GCN1 PF23271 541 - 662 9.8e-06 Stalled ribosome sensor GCN1-like, HEAT repeats
HEAT_ECM29 PF24492 727 - 886 3.9e-46 Proteasome adapter and scaffold protein ECM29, HEAT-repeat domain
ARM_ECM29_C PF23731 1057 - 1277 2.6e-06 ECM29, C-terminal ARM-like repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 491, 1335, 3120
Acc16I TGCGCA 1 cut(s) 594
Acc36I ACCTGC 3 cut(s) 4918, 5017, 5230
AccB1I GGYRCC 1 cut(s) 5197
AccB7I CCANNNNNTGG 1 cut(s) 3631
AccBSI CCGCTC 1 cut(s) 4859
AccI GTMKAC 2 cut(s) 1388, 1891
AccIII TCCGGA 1 cut(s) 260
AcoI YGGCCR 3 cut(s) 2398, 2785, 3491
AcuI CTGAAG 6 cut(s) 1136, 1661, 3021, 3239, 4707, 5241
AcyI GRCGYC 1 cut(s) 4292
AdeI CACNNNGTG 2 cut(s) 4652, 5194
AflIII ACRYGT 2 cut(s) 1608, 4417
AhdI GACNNNNNGTC 1 cut(s) 5299
AhlI ACTAGT 1 cut(s) 4042
AjnI CCWGG 8 cut(s) 577, 1661, 1809, 2445, 3444, 4509, 4597, 5050
AjuI GAANNNNNNNTTGG 6 cut(s) 393, 425, 2918, 2950, 4868, 4900
Alw21I GWGCWC 3 cut(s) 702, 1345, 2038
Ama87I CYCGRG 1 cut(s) 2608
Aor13HI TCCGGA 1 cut(s) 260
ApaI GGGCCC 1 cut(s) 1094
ArsI GACNNNNNNTTYG 4 cut(s) 2126, 2158, 2533, 2565
AseI ATTAAT 1 cut(s) 4977
Asp700I GAANNNNTTC 4 cut(s) 408, 1727, 2172, 3015
AspLEI GCGC 3 cut(s) 595, 2947, 3405
AspS9I GGNCC 9 cut(s) 52, 691, 1090, 1091, 2242, 3234, 3355, 3448, 3805
AsuC2I CCSGG 1 cut(s) 605
AsuII TTCGAA 1 cut(s) 3535
AvaI CYCGRG 1 cut(s) 2608
AvaII GGWCC 5 cut(s) 52, 2242, 3234, 3355, 3805
BaeGI GKGCMC 1 cut(s) 1094
BaeI ACNNNNGTAYC 2 cut(s) 3594, 3627
BalI TGGCCA 3 cut(s) 2400, 2787, 3493
BanI GGYRCC 1 cut(s) 5197
BanII GRGCYC 2 cut(s) 702, 1094
BarI GAAGNNNNNNTAC 2 cut(s) 3553, 3585
BauI CACGAG 1 cut(s) 3408
BbsI GAAGAC 3 cut(s) 9, 3009, 3341
Bbv12I GWGCWC 3 cut(s) 702, 1345, 2038
BbvCI CCTCAGC 1 cut(s) 1218
BccI CCATC 7 cut(s) 274, 455, 1425, 1550, 2796, 3091, 4301
BceAI ACGGC 2 cut(s) 143, 3543
BcgI CGANNNNNNTGC 6 cut(s) 502, 536, 3815, 3849, 4199, 4233
BciT130I CCWGG 8 cut(s) 579, 1663, 1811, 2447, 3446, 4511, 4599, 5052
BciVI GTATCC 1 cut(s) 3843
BclI TGATCA 2 cut(s) 3460, 5149
BcnI CCSGG 1 cut(s) 605
BcuI ACTAGT 1 cut(s) 4042
BfmI CTRYAG 5 cut(s) 1788, 2019, 2637, 3702, 4218
BfuAI ACCTGC 3 cut(s) 4918, 5017, 5230
BfuI GTATCC 1 cut(s) 3843
BglI GCCNNNNNGGC 1 cut(s) 4193
BglII AGATCT 1 cut(s) 3109
BmcAI AGTACT 1 cut(s) 4167
Bme1390I CCNGG 9 cut(s) 579, 605, 1663, 1811, 2447, 3446, 4511, 4599, 5052
Bme18I GGWCC 5 cut(s) 52, 2242, 3234, 3355, 3805
BmeRI GACNNNNNGTC 1 cut(s) 5299
BmeT110I CYCGRG 1 cut(s) 2608
BmgT120I GGNCC 9 cut(s) 52, 691, 1090, 1091, 2242, 3234, 3355, 3448, 3805
BmiI GGNNCC 4 cut(s) 693, 1092, 3966, 5199
BmrFI CCNGG 9 cut(s) 579, 605, 1663, 1811, 2447, 3446, 4511, 4599, 5052
BpiI GAAGAC 3 cut(s) 9, 3009, 3341
BplI GAGNNNNNCTC 6 cut(s) 2602, 2634, 2864, 2896, 3042, 3074
BpmI CTGGAG 7 cut(s) 873, 1153, 1388, 2468, 3564, 4412, 4559
Bpu10I CCTNAGC 3 cut(s) 696, 1218, 4986
Bpu14I TTCGAA 1 cut(s) 3535
BpuEI CTTGAG 6 cut(s) 486, 1367, 1672, 3821, 3887, 4233
BpuMI CCSGG 1 cut(s) 605
Bsa29I ATCGAT 1 cut(s) 1071
BsaBI GATNNNNATC 1 cut(s) 4311
BsaHI GRCGYC 1 cut(s) 4292
BsaI GGTCTC 5 cut(s) 1271, 1652, 2598, 4007, 5286
BsaWI WCCGGW 3 cut(s) 217, 260, 2536
BsaXI ACNNNNNCTCC 2 cut(s) 116, 146
Bse118I RCCGGY 2 cut(s) 54, 933
Bse1I ACTGG 7 cut(s) 2271, 3321, 3987, 4106, 4331, 4429, 4576
Bse3DI GCAATG 4 cut(s) 324, 1803, 2276, 3738
Bse8I GATNNNNATC 1 cut(s) 4311
BseAI TCCGGA 1 cut(s) 260
BseBI CCWGG 8 cut(s) 579, 1663, 1811, 2447, 3446, 4511, 4599, 5052
BseCI ATCGAT 1 cut(s) 1071
BseJI GATNNNNATC 1 cut(s) 4311
BseMI GCAATG 4 cut(s) 324, 1803, 2276, 3738
BseNI ACTGG 7 cut(s) 2271, 3321, 3987, 4106, 4331, 4429, 4576
BseRI GAGGAG 7 cut(s) 107, 124, 133, 142, 774, 779, 4820
BseSI GKGCMC 1 cut(s) 1094
BseYI CCCAGC 1 cut(s) 3845
BsgI GTGCAG 5 cut(s) 1968, 3305, 4182, 4281, 4629
BshNI GGYRCC 1 cut(s) 5197
BshVI ATCGAT 1 cut(s) 1071
BsiHKAI GWGCWC 3 cut(s) 702, 1345, 2038
BsiHKCI CYCGRG 1 cut(s) 2608
BsiSI CCGG 7 cut(s) 55, 218, 261, 604, 899, 934, 2537
BslFI GGGAC 1 cut(s) 3114
BsmBI CGTCTC 1 cut(s) 1259
BsmFI GGGAC 1 cut(s) 3114
BsmI GAATGC 4 cut(s) 297, 575, 3500, 5219
Bso31I GGTCTC 5 cut(s) 1271, 1652, 2598, 4007, 5286
BsoBI CYCGRG 1 cut(s) 2608
Bsp119I TTCGAA 1 cut(s) 3535
Bsp120I GGGCCC 1 cut(s) 1090
Bsp1286I GDGCHC 4 cut(s) 702, 1094, 1345, 2038
Bsp13I TCCGGA 1 cut(s) 260
Bsp1407I TGTACA 1 cut(s) 5320
Bsp19I CCATGG 6 cut(s) 681, 1931, 2580, 2673, 3510, 5060
BspDI ATCGAT 1 cut(s) 1071
BspEI TCCGGA 1 cut(s) 260
BspHI TCATGA 2 cut(s) 5002, 5277
BspLI GGNNCC 4 cut(s) 693, 1092, 3966, 5199
BspMAI CTGCAG 1 cut(s) 4222
BspMI ACCTGC 3 cut(s) 4918, 5017, 5230
BspQI GCTCTTC 1 cut(s) 4392
BspT104I TTCGAA 1 cut(s) 3535
BspT107I GGYRCC 1 cut(s) 5197
BspTNI GGTCTC 5 cut(s) 1271, 1652, 2598, 4007, 5286
BsrBI CCGCTC 1 cut(s) 4859
BsrDI GCAATG 4 cut(s) 324, 1803, 2276, 3738
BsrFI RCCGGY 2 cut(s) 54, 933
BsrGI TGTACA 1 cut(s) 5320
BsrI ACTGG 7 cut(s) 2271, 3321, 3987, 4106, 4331, 4429, 4576
BssAI RCCGGY 2 cut(s) 54, 933
BssNAI GTATAC 2 cut(s) 1389, 1892
BssNI GRCGYC 1 cut(s) 4292
BssSI CACGAG 1 cut(s) 3408
Bst1107I GTATAC 2 cut(s) 1389, 1892
Bst2BI CACGAG 1 cut(s) 3408
Bst2UI CCWGG 8 cut(s) 579, 1663, 1811, 2447, 3446, 4511, 4599, 5052
Bst6I CTCTTC 6 cut(s) 27, 36, 773, 4380, 4392, 4905
BstACI GRCGYC 1 cut(s) 4292
BstAPI GCANNNNNTGC 5 cut(s) 1874, 2111, 3212, 3506, 3842
BstAUI TGTACA 1 cut(s) 5320
BstBI TTCGAA 1 cut(s) 3535
BstDSI CCRYGG 6 cut(s) 681, 1931, 2580, 2673, 3510, 5060
BstEII GGTNACC 1 cut(s) 4235
BstENI CCTNNNNNAGG 1 cut(s) 2062
BstHHI GCGC 3 cut(s) 595, 2947, 3405
BstNI CCWGG 8 cut(s) 579, 1663, 1811, 2447, 3446, 4511, 4599, 5052
BstNSI RCATGY 8 cut(s) 1612, 1820, 2433, 3781, 3836, 4161, 4232, 4731
BstPI GGTNACC 1 cut(s) 4235
BstSCI CCNGG 9 cut(s) 577, 603, 1661, 1809, 2445, 3444, 4509, 4597, 5050
BstSFI CTRYAG 5 cut(s) 1788, 2019, 2637, 3702, 4218
BstSLI GKGCMC 1 cut(s) 1094
BstV2I GAAGAC 3 cut(s) 9, 3009, 3341
BstX2I RGATCY 8 cut(s) 660, 1363, 1835, 1883, 3109, 3130, 3389, 3634
BstXI CCANNNNNNTGG 2 cut(s) 367, 688
BstYI RGATCY 8 cut(s) 660, 1363, 1835, 1883, 3109, 3130, 3389, 3634
BstZ17I GTATAC 2 cut(s) 1389, 1892
Bsu15I ATCGAT 1 cut(s) 1071
BsuI GTATCC 1 cut(s) 3843
BsuTUI ATCGAT 1 cut(s) 1071
BtgI CCRYGG 6 cut(s) 681, 1931, 2580, 2673, 3510, 5060
BtsI GCAGTG 3 cut(s) 1128, 1326, 4215
BveI ACCTGC 3 cut(s) 4918, 5017, 5230
CciI TCATGA 2 cut(s) 5002, 5277
CfoI GCGC 3 cut(s) 595, 2947, 3405
Cfr10I RCCGGY 2 cut(s) 54, 933
Cfr13I GGNCC 9 cut(s) 52, 691, 1090, 1091, 2242, 3234, 3355, 3448, 3805
ClaI ATCGAT 1 cut(s) 1071
CseI GACGC 3 cut(s) 1241, 2907, 4281
DraI TTTAAA 2 cut(s) 1057, 4173
DraIII CACNNNGTG 2 cut(s) 4652, 5194
DriI GACNNNNNGTC 1 cut(s) 5299
EaeI YGGCCR 3 cut(s) 2398, 2785, 3491
Eam1104I CTCTTC 6 cut(s) 27, 36, 773, 4380, 4392, 4905
Eam1105I GACNNNNNGTC 1 cut(s) 5299
EarI CTCTTC 6 cut(s) 27, 36, 773, 4380, 4392, 4905
EciI GGCGGA 2 cut(s) 20, 145
Ecl136II GAGCTC 1 cut(s) 700
Eco147I AGGCCT 2 cut(s) 1536, 4793
Eco24I GRGCYC 2 cut(s) 702, 1094
Eco31I GGTCTC 5 cut(s) 1271, 1652, 2598, 4007, 5286
Eco32I GATATC 4 cut(s) 2043, 2488, 3439, 4313
Eco47I GGWCC 5 cut(s) 52, 2242, 3234, 3355, 3805
Eco53kI GAGCTC 1 cut(s) 700
Eco57I CTGAAG 6 cut(s) 1136, 1661, 3021, 3239, 4707, 5241
Eco88I CYCGRG 1 cut(s) 2608
Eco91I GGTNACC 1 cut(s) 4235
EcoICRI GAGCTC 1 cut(s) 700
EcoNI CCTNNNNNAGG 1 cut(s) 2062
EcoO109I RGGNCCY 1 cut(s) 1091
EcoO65I GGTNACC 1 cut(s) 4235
EcoRI GAATTC 3 cut(s) 1037, 1110, 3157
EcoRII CCWGG 8 cut(s) 577, 1661, 1809, 2445, 3444, 4509, 4597, 5050
EcoRV GATATC 4 cut(s) 2043, 2488, 3439, 4313
EcoT22I ATGCAT 5 cut(s) 992, 1267, 1870, 4159, 5219
EcoT38I GRGCYC 2 cut(s) 702, 1094
Esp3I CGTCTC 1 cut(s) 1259
FalI AAGNNNNNCTT 4 cut(s) 886, 918, 3212, 3244
FaqI GGGAC 1 cut(s) 3114
FauI CCCGC 1 cut(s) 74
FauNDI CATATG 2 cut(s) 1828, 3305
FbaI TGATCA 2 cut(s) 3460, 5149
FblI GTMKAC 2 cut(s) 1388, 1891
FriOI GRGCYC 2 cut(s) 702, 1094
FspI TGCGCA 1 cut(s) 594
GlaI GCGC 3 cut(s) 594, 2946, 3404
GsaI CCCAGC 1 cut(s) 3849
GsuI CTGGAG 7 cut(s) 873, 1153, 1388, 2468, 3564, 4412, 4559
HapII CCGG 7 cut(s) 55, 218, 261, 604, 899, 934, 2537
HgaI GACGC 3 cut(s) 1241, 2907, 4281
HhaI GCGC 3 cut(s) 595, 2947, 3405
Hin1I GRCGYC 1 cut(s) 4292
Hin6I GCGC 3 cut(s) 593, 2945, 3403
HinP1I GCGC 3 cut(s) 593, 2945, 3403
HincII GTYRAC 1 cut(s) 3980
HindII GTYRAC 1 cut(s) 3980
HindIII AAGCTT 7 cut(s) 383, 1673, 2699, 2993, 3719, 3863, 5282
HpaII CCGG 7 cut(s) 55, 218, 261, 604, 899, 934, 2537
Hpy166II GTNNAC 9 cut(s) 196, 1389, 1892, 2953, 3752, 3980, 4415, 5116, 5336
Hpy8I GTNNAC 9 cut(s) 196, 1389, 1892, 2953, 3752, 3980, 4415, 5116, 5336
Hpy99I CGWCG 4 cut(s) 86, 2923, 3827, 4214
HpyCH4IV ACGT 6 cut(s) 1694, 1912, 1987, 3084, 3822, 4417
HpySE526I ACGT 6 cut(s) 1694, 1912, 1987, 3084, 3822, 4417
Hsp92I GRCGYC 1 cut(s) 4292
HspAI GCGC 3 cut(s) 593, 2945, 3403
Kpn2I TCCGGA 1 cut(s) 260
Ksp22I TGATCA 2 cut(s) 3460, 5149
LguI GCTCTTC 1 cut(s) 4392
MaeII ACGT 6 cut(s) 1694, 1912, 1987, 3084, 3822, 4417
MbiI CCGCTC 1 cut(s) 4859
MfeI CAATTG 3 cut(s) 588, 1878, 3959
MflI RGATCY 8 cut(s) 660, 1363, 1835, 1883, 3109, 3130, 3389, 3634
MhlI GDGCHC 4 cut(s) 702, 1094, 1345, 2038
MlsI TGGCCA 3 cut(s) 2400, 2787, 3493
MluNI TGGCCA 3 cut(s) 2400, 2787, 3493
MmeI TCCRAC 2 cut(s) 105, 121
Mox20I TGGCCA 3 cut(s) 2400, 2787, 3493
Mph1103I ATGCAT 5 cut(s) 992, 1267, 1870, 4159, 5219
MroI TCCGGA 1 cut(s) 260
MroXI GAANNNNTTC 4 cut(s) 408, 1727, 2172, 3015
MscI TGGCCA 3 cut(s) 2400, 2787, 3493
MslI CAYNNNNRTG 8 cut(s) 311, 450, 1247, 2428, 3304, 4233, 4979, 5349
Msp20I TGGCCA 3 cut(s) 2400, 2787, 3493
MspA1I CMGCKG 1 cut(s) 1655
MspI CCGG 7 cut(s) 55, 218, 261, 604, 899, 934, 2537
MspR9I CCNGG 9 cut(s) 579, 605, 1663, 1811, 2447, 3446, 4511, 4599, 5052
MunI CAATTG 3 cut(s) 588, 1878, 3959
Mva1269I GAATGC 4 cut(s) 297, 575, 3500, 5219
MvaI CCWGG 8 cut(s) 579, 1663, 1811, 2447, 3446, 4511, 4599, 5052
NciI CCSGG 1 cut(s) 605
NcoI CCATGG 6 cut(s) 681, 1931, 2580, 2673, 3510, 5060
NdeI CATATG 2 cut(s) 1828, 3305
NlaIV GGNNCC 4 cut(s) 693, 1092, 3966, 5199
NsbI TGCGCA 1 cut(s) 594
NsiI ATGCAT 5 cut(s) 992, 1267, 1870, 4159, 5219
NspI RCATGY 8 cut(s) 1612, 1820, 2433, 3781, 3836, 4161, 4232, 4731
NspV TTCGAA 1 cut(s) 3535
PaeI GCATGC 4 cut(s) 3781, 3836, 4232, 4731
PagI TCATGA 2 cut(s) 5002, 5277
PceI AGGCCT 2 cut(s) 1536, 4793
PciI ACATGT 1 cut(s) 1608
PciSI GCTCTTC 1 cut(s) 4392
PcsI WCGNNNNNNNCGW 1 cut(s) 78
PctI GAATGC 4 cut(s) 297, 575, 3500, 5219
PdmI GAANNNNTTC 4 cut(s) 408, 1727, 2172, 3015
PflMI CCANNNNNTGG 1 cut(s) 3631
PfoI TCCNGGA 1 cut(s) 1809
PscI ACATGT 1 cut(s) 1608
PshBI ATTAAT 1 cut(s) 4977
PsiI TTATAA 3 cut(s) 491, 1335, 3120
Psp124BI GAGCTC 1 cut(s) 702
Psp6I CCWGG 8 cut(s) 577, 1661, 1809, 2445, 3444, 4509, 4597, 5050
PspEI GGTNACC 1 cut(s) 4235
PspFI CCCAGC 1 cut(s) 3845
PspGI CCWGG 8 cut(s) 577, 1661, 1809, 2445, 3444, 4509, 4597, 5050
PspN4I GGNNCC 4 cut(s) 693, 1092, 3966, 5199
PspOMI GGGCCC 1 cut(s) 1090
PspPI GGNCC 9 cut(s) 52, 691, 1090, 1091, 2242, 3234, 3355, 3448, 3805
PstI CTGCAG 1 cut(s) 4222
PsuI RGATCY 8 cut(s) 660, 1363, 1835, 1883, 3109, 3130, 3389, 3634
PvuII CAGCTG 1 cut(s) 1655
RseI CAYNNNNRTG 8 cut(s) 311, 450, 1247, 2428, 3304, 4233, 4979, 5349
SacI GAGCTC 1 cut(s) 702
SapI GCTCTTC 1 cut(s) 4392
Sau96I GGNCC 9 cut(s) 52, 691, 1090, 1091, 2242, 3234, 3355, 3448, 3805
ScaI AGTACT 1 cut(s) 4167
ScrFI CCNGG 9 cut(s) 579, 605, 1663, 1811, 2447, 3446, 4511, 4599, 5052
SduI GDGCHC 4 cut(s) 702, 1094, 1345, 2038
SfcI CTRYAG 5 cut(s) 1788, 2019, 2637, 3702, 4218
SfuI TTCGAA 1 cut(s) 3535
SinI GGWCC 5 cut(s) 52, 2242, 3234, 3355, 3805
SmiMI CAYNNNNRTG 8 cut(s) 311, 450, 1247, 2428, 3304, 4233, 4979, 5349
SmlI CTYRAG 6 cut(s) 501, 1382, 1687, 3800, 3866, 4248
SmoI CTYRAG 6 cut(s) 501, 1382, 1687, 3800, 3866, 4248
SpeI ACTAGT 1 cut(s) 4042
SphI GCATGC 4 cut(s) 3781, 3836, 4232, 4731
SseBI AGGCCT 2 cut(s) 1536, 4793
SspI AATATT 3 cut(s) 1286, 2440, 2457
SstI GAGCTC 1 cut(s) 702
StuI AGGCCT 2 cut(s) 1536, 4793
StyD4I CCNGG 9 cut(s) 577, 603, 1661, 1809, 2445, 3444, 4509, 4597, 5050
TaiI ACGT 6 cut(s) 1697, 1915, 1990, 3087, 3825, 4420
TaqII GACCGA 2 cut(s) 4254, 5289
TatI WGTACW 5 cut(s) 2723, 3062, 4165, 5312, 5320
TauI GCSGC 2 cut(s) 1982, 3453
TspGWI ACGGA 3 cut(s) 1999, 3494, 3737
Van91I CCANNNNNTGG 1 cut(s) 3631
VpaK11BI GGWCC 5 cut(s) 52, 2242, 3234, 3355, 3805
VspI ATTAAT 1 cut(s) 4977
XagI CCTNNNNNAGG 1 cut(s) 2062
XbaI TCTAGA 2 cut(s) 878, 2144
XceI RCATGY 8 cut(s) 1612, 1820, 2433, 3781, 3836, 4161, 4232, 4731
XcmI CCANNNNNNNNNTGG 1 cut(s) 367
XmiI GTMKAC 2 cut(s) 1388, 1891
XmnI GAANNNNTTC 4 cut(s) 408, 1727, 2172, 3015
ZrmI AGTACT 1 cut(s) 4167
Zsp2I ATGCAT 5 cut(s) 992, 1267, 1870, 4159, 5219
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.