RchiOBHm_Chr5g0051871

Proteasome-associated protein ECM29 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0006358
Physical Location & Seq
Reverse (-)
53270066 .. 53295822
25757 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ32931

Sequence Viewer

Length: 5391 bp
ATGGCGGAAACACCATCGTCGGTATCATCTTCCAAATCGGATGAAGAGAAAGAAGAGATGCTCGACCGGTTGCTCACCCGCCTCGCTCTCCGTGACGACTCCAACCTCCAACCTCTACTCTCCAAGCTCCTCCCCTATACTATTTCCTCCCTTTCTTCCAACTCCTCCGCCGTCCGGAACAAGGTGCTAGAAATACTTAGTCATGTGAACAAGCGAGTGAAGCATCAACCTGAGATTGGTTTGCCCTTGACTGAACTATGGACTATATACTCGGAAGCTCATTCTGCTCCAATCGTGAGGAATGTTTGCATTCTCTACCTCGAGATGGCAATGGATCGTGCCGATAGAAAGGAGAAGGAAAATTTGTCACCCATGCTTCTGTTGGGCGTTTCCAAGCTTCCTCAACAGCACCAAGAAATTATACTCAGACTTGTTATAAAGGTCATTGGTGAACGTCACACAAGCGGAATAGATGGAGAAGTTGCTGCAAAGTATGGACTGATAAACGATTCTCAAGACAGAAACGTTTTTATCGAATTTTGCCTTCACACAATCTTATATCAGCCATCATCTCAAAGAGAATGCCCGCCAGGACTTTCAATTGCTCAAGCCAACCGCGTAACAGCTAGACAATCATTGAACAGTGATATGCTTCTAACAAGAAAGTTAGGGATCTTGAATGTTGTTGAGGCCATGGAGCTGGCCCCTGAGCTCGTTTATCCTTTCTACCTGACTGCCTCCATAGATTGTCAAGAGCTTGTCGTCAAGAGAGGAGAGGAGCTTGTAAAGAAGAAGGCTGCTGGAGCTAATTCAGATGATTCAGTCCTCATAAACAGACTTTTTTTGTTATTTAATGGAACTGCTACATCTCAAAATGTTGGTCCTGAATCTAGAGTCACTCCAGCAAGTCCTGCTTTGAAAGGAAAAATGATGTCTATTTTCTGCCGGTCTATTACAGCCGCTAACAGTTTCCCTTCAACCTTGCAGTGCATTTTTGGTTGCATCTATGGAAGTGATACCACATCAAGGTTGAAGCAGTTGGGAATGGAATTCACTGTCTGGGTATTTAAACATTCCATAATCGATCAACTTAAACTTATGGGCCCTGTCATTCTGAGTGGAATACTGAAGTCACTTGACACTGCTTCAAGTTCAGAATCAGATGCTACTCATAGGGACTCCAGAACTTTCGCTTATCAAGCTATTGGATTGCTTGCACAGCGGATGCCTCAGCTTTTCAGAGATACGACTGACATGGCTGTGCGTCTTTTTGATGCATTGAAGGTAGAGACCCAATATTTTCGCCTCAGTATTCAAGAAGCAACAAATTCTCTTTCCACTGCGTATAAGGAACAAAGTGAAGTAAGATTCTGCGCTATAAGATGGGCGACTTCTTTATTTGACTTACAACATTGTCCAAGCCGGTATATCTGTATGCTTGGGGCTGCTGATATTAAATTGGATATAAGGGAGATTGCATTTGAAGGCCTTTTTCCTATGAAAGATGATGGGTCAAGTATGAGTGAGCAGATACAAGAGCTACATTACCCCAAACTTGGAGACATGTTAGATTATATTCTTTCACATCGGCCAAACTTGTCAGAACCAGCTGAGATGCGGGAGCAGAAGCTTCAGTTTCCCTCAAAAACGTATCTAGTTATTATTGAATTTTTGTTGAAGTGCTTTGAGTCAGAATTGGAGCATAATAATTCAATTAAAGGATCAACTCAGTTTCACTCGTCTGTAGAAGCAATGTGCTTACTTCTGGAACATGCTATGGCATATGAAGGGTCTGTTGAGTTGCATGCCAAGGCTTCCAATGCATTGATTGCGATTGGATCTCATATTCCAGAGCTTGTAGCTTCACGTTATGCAAAAAAAGTTCAATGGCTAAAACAATTATTGAGTCATATTGACTTAGATACCCGTGAAGCTGCAGCACGATTACTTGGTATTGCTTCCTCCGTTCTTCCTCCAGCTGCTTCATGTGATCTTATTTCTGAAATAATTGCCTCAGTAAGGGGAATACATAAGTTAAGGTTTGAGGTTCAACACGGAGCATTATGTGCATTAGGTTATGTAACTGCAGATTGTATGTCTAGAAAACCAGCTATCCCAGAGGCATTATTTCAAACTACCCTAAAGTTACTTGTCGATGTTGTTAATTCTGAGACTGCGACACTAGCATCAGTAGCGGTCCAAGCATTAGGTCATATTGGACTGATCGTTGCATTGCCTTCACTTATTATTGAGTCATGTTCAGTTGATATTCTGGTGCTTTTACAAGAAAGATTGCGAAAGCTAGTAAAGGGTGATGATACCAAAGCAATTCAGAAAATTATTATATCCATTGGCCACATCTGTGTCAAGGAAACATCATCCGCGTGTCTTAATATTGCCCTGGAGTTGATATTCAGTCTTTCCCGATCTAAGGTTGAGGATATCCTCTTTGCCGCTGGCGAGGCACTGTCGTTTTTGTGGCGTGGTGTTCCGGTGACTACTGATTTGATTCTCACCACTAATTATTCACTCTCCATGGCTTCTAAGTTTCTCATGGGAGATGCGAGTTTATCTCTCTCAAAACATAGCCCCATGGAAATGAATGAAGCTAATAAAGATCGTGATGACATGGTTCGAGAAGCAATTACTAAAAAGCTTTTTGACGATCTTCTATACAGTACAAGAAAAGAAGATCGCTGTGCTGGAACTGTATGGCTATTATCAATTACCATGTATTGTGGCCATCAACGAGCTATTCAGAAAATGCTGCCAGAAATTCAGGAGGCTTTTTCACACCTTCTTGGTGAACAAAATGAACTTACGCAGGAGCTGGCCTCACAAGGGATGAGTGTTGTGTATGAAATTGGTGATGCATCAATGAAAGGAAACTTGGTAAATGCACTTGTAAACACTCTGACAGGTTCCGGGAAGAGAAAAAGGGCTATAAAGCTTGCTGAAGACTCCGTTCTTCCAAAGGGAGTTATTGGTGAAGGTCTTAGTGGAGGCAAACTCAGTACATACAAGGAGCTGTGCTATGTAGCGAATGAGATGGGACAACCAGATCTGATTTATAAGTTTATGGATCTGGCTAATTATCAAACTTCTCTGAATTCAAAGAGGGGTGCTGCCTTTGGTTTTTCAAAGATAGCAAAGCAAGCAGGAGATGCCCTGAAGCCACACCTGCGGTCCTTAATTCCAAGGCTTGTTCGTTATCAGTACGATCCTGACAAAAATGTGCAGGATGCTATGTCACACATATGGAAGTCACTGGTGGAAGATTCAAAGAAAACCATTGATGAACACTTGGACCTCATTATTGATGACTTGTTAATACAATGTGGATCTAGGATTTGGCGCACACAAGAGGCATCCTGTCTTGCCCTTGCAGATATTATCCAGGGCCGGAAGTTTGATCAGGTTGGGAAGCATTTAAGGAAGTTATGGTCAGCAGCGTTCCATGCCATGAATGATATTAAAGAGACTGTTCGAAATTCTGGCAACAAGTTATGCCGTGTTTTAACTTCATTGACGGTGAGGCTTTCTGATGTCACCCTAACTGATGTATCAGATGCAAGCCAAACAATGGATCTTGTTCTGCCTCTTTTGCTTACAGAAGGCATACTAAGTAAAGTTGATAGTATTCGAAAGGCATCTATAGTGGTTGTTATGAAGCTTGCAAAGGGGGCTGGCATTGCAATTCGTCCACATTTATCTGATCTAGTTTGTTGCATGCTGGAAAGTTTATCGAGCCTGGAGGACCAAGGGCTTAATTACGTGGAGTTGCATGCAGCAAATGCTGGGATACAGACAGAGAAGCTCGAAAGTTTGAGAATTTCGATCTCAAAAGGCTCTCCCATGTGGGAAACTCTTGATCTCTGCATAAAAGTCGTGGACGCAGGATCATTAGATCAATTGGTTCCTCGTCTTGGTCAATTGGTTCGGTCTGGGGTTGGTCTCAATACAAGGGTTGGTGTTGCCAGCTTTATTACCTTACTAGTTCAAGAAGTTGGTGTTGAAATCAAACCATATACGAGCAAGCTACTGAGGCTGTTGTTTCCAGTTGTGGAGAACGAAAAAAGTGCTGCATCAAAACGAGCCTTTGCTGATGCATGTGCAGTACTTTTAAAACATACAGTAGCCTCTCAGGCCGAAAAGTTGATCGATGACACTGCAGCTTTGCATATACGTGACCGAAATGCTCAAGTGGCGTGTGCGGTTTTATTGAAAAGCTATTCATCCAAGGCCTCAGACATTTTGGATGGATATCTTGCAGCTATACTTCCAGTAATTTTTATCTCAAGGTTTGACGATGACAAGTATGTTTCTGGCTCGTTTGAAGAGTTGTGGGAAGAGCATACAAGTAGTGAACGTGTTGCCCTCCAGTTATACTTGGCAGAAATTGTTTCTCTTATTTGTGAGAGCATTGCAACATCTTCTTGGGCAAGTAAAAAGAAGTCAGCCCAGGCTATTTCCAAACTCAGTGAAGTTTTGGGTGAATCCCTGGCTTCTTACTATAATGTCTTGCTCAAATCTCTTATGAAAGAGATTCCTGGTCGGTTATGGGAGGGAAAGGAAGCTCTATTGTATTCAATTGCTGCACTTTCTGTGTCTTGTCATAAAGCAATATCAACTGATGATCCTCATACTCTGAATGAAGTTTTGAGGGTATTATCTTCAGCATGCATGAAAAAGGCAAAGAAATATCGTGAAGCAGCTCTATCGTGTCTTGAACAGGTTGTAAAAGCCTTTGGTAATCAAGAGTTCTTTAATGAGGCATTTCCATTGTTATACGATATGTGTAATGCTTCCACTCTCAGTGTTTCTGGGAAAGCAACTTTGGCTGGCAATGGTGCTAAAGCAGATGAGGATCAAATTGAGCAGGTTCCTGTTTCACATGAGAAAATCTTGGACTGCATGACCGCTTGCATTCATGTGGCTAATATAAAAGATATTCTTGAACAACATAAGAACTTGATGCAAGTGTTGACTACTGCTCTGTCACCTGGATTTCCATGGACAGTCAAAATATCTGCCCTTTCATTGATAAAGGAACTTGTTTCAAGTTACCAGAAGGTTCTGGGTGACTCCAAAGAAACTTACGCTCATGCCAACATAATTTTGTTAGTTCGGGAGTTGTTTCACTCAGTGGCACCTCAAGTTGTCGAATGCATAAGCACAATTAAAGTTGGGCAGGTTCATGTAACTGCTTCAGAGTGCCTTCTAGTGATCATCACGCTTTATGGAGACCTTCGGTCAATTGACTCTACTAATGTACAATTTGGGGGTACGCTTCTGCATCTGTACGAAGTGGAGAAAAATGGCGAAGCAAAGTCCTTGTTGAAAAAATGTATGGATACCCTTGAAAACTTAAAACCAGAATGCCCGAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ecm29

Protein Analysis

1796

Amino Acids

198.22

Weight (kDa)

6.33

Isoelectric Point (pI)

40.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ECM29_N PF13001 21 - 481 6.3e-115 Proteasome component ECM29, N-terminal
ARM_ECM29 PF23702 607 - 736 1.8e-12 ECM29, ARM-like repeats
HEAT_GCN1 PF23271 1047 - 1175 9e-07 Stalled ribosome sensor GCN1-like, HEAT repeats
HEAT_ECM29 PF24492 1233 - 1392 2.3e-46 Proteasome adapter and scaffold protein ECM29, HEAT-repeat domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 437, 3072
AarI CACCTGC 1 cut(s) 3189
Acc36I ACCTGC 3 cut(s) 3189, 4870, 5182
AccB1I GGYRCC 1 cut(s) 5149
AccB7I CCANNNNNTGG 1 cut(s) 3583
AccII CGCG 2 cut(s) 618, 2384
AccIII TCCGGA 1 cut(s) 174
AclI AACGTT 1 cut(s) 525
AcoI YGGCCR 3 cut(s) 1589, 2353, 2740
AcsI RAATTY 9 cut(s) 361, 536, 1049, 1327, 1667, 2775, 3109, 3490, 3831
AcuI CTGAAG 6 cut(s) 1148, 1616, 2976, 3191, 4659, 5193
AdeI CACNNNGTG 1 cut(s) 5146
AfaI GTAC 7 cut(s) 2680, 3016, 3218, 4119, 5274, 5287, 5303
AflIII ACRYGT 2 cut(s) 1563, 4369
AgeI ACCGGT 1 cut(s) 66
AhdI GACNNNNNGTC 1 cut(s) 5251
AhlI ACTAGT 1 cut(s) 3994
AjnI CCWGG 8 cut(s) 589, 2400, 3396, 3750, 4461, 4500, 4549, 5002
AjuI GAANNNNNNNTTGG 4 cut(s) 2873, 2905, 4820, 4852
AleI CACNNNNGTG 1 cut(s) 2361
Alw21I GWGCWC 1 cut(s) 714
Alw26I GTCTC 6 cut(s) 1283, 1554, 2165, 3473, 3959, 5238
AlwNI CAGNNNCTG 1 cut(s) 2830
Ama87I CYCGRG 2 cut(s) 320, 5383
Aor13HI TCCGGA 1 cut(s) 174
ApaI GGGCCC 1 cut(s) 1106
ApoI RAATTY 9 cut(s) 361, 536, 1049, 1327, 1667, 2775, 3109, 3490, 3831
ArsI GACNNNNNNTTYG 4 cut(s) 865, 897, 2488, 2520
AsiGI ACCGGT 1 cut(s) 66
AspLEI GCGC 2 cut(s) 1376, 3357
AspS9I GGNCC 9 cut(s) 703, 881, 1102, 1103, 2197, 3186, 3307, 3400, 3757
AsuC2I CCSGG 1 cut(s) 2926
AsuII TTCGAA 2 cut(s) 3487, 3643
AvaI CYCGRG 2 cut(s) 320, 5383
AvaII GGWCC 5 cut(s) 881, 2197, 3186, 3307, 3757
BaeGI GKGCMC 1 cut(s) 1106
BaeI ACNNNNGTAYC 2 cut(s) 3546, 3579
BalI TGGCCA 2 cut(s) 2355, 2742
BanI GGYRCC 1 cut(s) 5149
BanII GRGCYC 2 cut(s) 714, 1106
BbsI GAAGAC 1 cut(s) 2964
Bbv12I GWGCWC 1 cut(s) 714
BbvCI CCTCAGC 1 cut(s) 1230
BccI CCATC 9 cut(s) 22, 319, 467, 574, 1377, 1502, 2751, 3043, 4253
BceAI ACGGC 2 cut(s) 155, 3495
BcgI CGANNNNNNTGC 8 cut(s) 3868, 3902, 4061, 4095, 4151, 4185, 4701, 4735
BciT130I CCWGG 8 cut(s) 591, 2402, 3398, 3752, 4463, 4502, 4551, 5004
BciVI GTATCC 2 cut(s) 3795, 5347
BclI TGATCA 2 cut(s) 3412, 5226
BcnI CCSGG 1 cut(s) 2926
BcoDI GTCTC 6 cut(s) 1283, 1554, 2165, 3473, 3959, 5238
BcuI ACTAGT 1 cut(s) 3994
BfmI CTRYAG 5 cut(s) 1743, 1935, 2085, 3654, 4170
BfuAI ACCTGC 3 cut(s) 3189, 4870, 5182
BfuI GTATCC 2 cut(s) 3795, 5347
BglI GCCNNNNNGGC 1 cut(s) 4145
BglII AGATCT 1 cut(s) 3061
BmcAI AGTACT 1 cut(s) 4119
Bme1390I CCNGG 9 cut(s) 591, 2402, 2926, 3398, 3752, 4463, 4502, 4551, 5004
Bme18I GGWCC 5 cut(s) 881, 2197, 3186, 3307, 3757
BmeRI GACNNNNNGTC 1 cut(s) 5251
BmeT110I CYCGRG 2 cut(s) 320, 5383
BmgT120I GGNCC 9 cut(s) 703, 881, 1102, 1103, 2197, 3186, 3307, 3400, 3757
BmiI GGNNCC 6 cut(s) 705, 1104, 2923, 3918, 4884, 5151
BmrFI CCNGG 9 cut(s) 591, 2402, 2926, 3398, 3752, 4463, 4502, 4551, 5004
BpiI GAAGAC 1 cut(s) 2964
BplI GAGNNNNNCTC 6 cut(s) 2557, 2589, 2819, 2851, 2994, 3026
BpmI CTGGAG 7 cut(s) 822, 885, 1165, 1959, 2423, 3773, 4364
Bpu10I CCTNAGC 2 cut(s) 708, 1230
Bpu14I TTCGAA 2 cut(s) 3487, 3643
BpuEI CTTGAG 5 cut(s) 498, 591, 4185, 4282, 5139
BpuMI CCSGG 1 cut(s) 2926
Bsa29I ATCGAT 2 cut(s) 1083, 4161
BsaAI YACGTR 2 cut(s) 3775, 4187
BsaBI GATNNNNATC 2 cut(s) 4263, 4866
BsaI GGTCTC 3 cut(s) 1283, 3959, 5238
BsaWI WCCGGW 3 cut(s) 66, 174, 2491
Bse118I RCCGGY 3 cut(s) 66, 945, 1422
Bse1I ACTGG 4 cut(s) 3273, 4058, 4283, 4381
Bse3DI GCAATG 6 cut(s) 336, 1758, 2231, 3690, 4422, 4852
Bse8I GATNNNNATC 2 cut(s) 4263, 4866
BseAI TCCGGA 1 cut(s) 174
BseBI CCWGG 8 cut(s) 591, 2402, 3398, 3752, 4463, 4502, 4551, 5004
BseCI ATCGAT 2 cut(s) 1083, 4161
BseGI GGATG 8 cut(s) 46, 1230, 2378, 2850, 3247, 3368, 4235, 4264
BseJI GATNNNNATC 2 cut(s) 4263, 4866
BseMI GCAATG 6 cut(s) 336, 1758, 2231, 3690, 4422, 4852
BseNI ACTGG 4 cut(s) 3273, 4058, 4283, 4381
BseRI GAGGAG 4 cut(s) 119, 154, 786, 791
BseSI GKGCMC 1 cut(s) 1106
BseYI CCCAGC 1 cut(s) 3797
BsgI GTGCAG 3 cut(s) 3257, 4134, 4581
Bsh1236I CGCG 2 cut(s) 618, 2384
Bsh1285I CGRYCG 1 cut(s) 67
BshNI GGYRCC 1 cut(s) 5149
BshTI ACCGGT 1 cut(s) 66
BshVI ATCGAT 2 cut(s) 1083, 4161
BsiEI CGRYCG 1 cut(s) 67
BsiHKAI GWGCWC 1 cut(s) 714
BsiHKCI CYCGRG 2 cut(s) 320, 5383
BsiSI CCGG 7 cut(s) 67, 175, 946, 1423, 2492, 2925, 3403
BslFI GGGAC 2 cut(s) 1190, 3066
BsmAI GTCTC 6 cut(s) 1283, 1554, 2165, 3473, 3959, 5238
BsmFI GGGAC 2 cut(s) 1190, 3066
BsmI GAATGC 5 cut(s) 309, 587, 4926, 5171, 5384
Bso31I GGTCTC 3 cut(s) 1283, 3959, 5238
BsoBI CYCGRG 2 cut(s) 320, 5383
Bsp119I TTCGAA 2 cut(s) 3487, 3643
Bsp120I GGGCCC 1 cut(s) 1102
Bsp1286I GDGCHC 2 cut(s) 714, 1106
Bsp13I TCCGGA 1 cut(s) 174
Bsp1407I TGTACA 1 cut(s) 5272
Bsp19I CCATGG 4 cut(s) 693, 2535, 2592, 5012
BspDI ATCGAT 2 cut(s) 1083, 4161
BspEI TCCGGA 1 cut(s) 174
BspFNI CGCG 2 cut(s) 618, 2384
BspLI GGNNCC 6 cut(s) 705, 1104, 2923, 3918, 4884, 5151
BspMAI CTGCAG 3 cut(s) 1939, 2089, 4174
BspMI ACCTGC 3 cut(s) 3189, 4870, 5182
BspQI GCTCTTC 1 cut(s) 4344
BspT104I TTCGAA 2 cut(s) 3487, 3643
BspT107I GGYRCC 1 cut(s) 5149
BspTNI GGTCTC 3 cut(s) 1283, 3959, 5238
BsrDI GCAATG 3 cut(s) 336, 1758, 2231
BsrFI RCCGGY 3 cut(s) 66, 945, 1422
BsrGI TGTACA 1 cut(s) 5272
BsrI ACTGG 4 cut(s) 3273, 4058, 4283, 4381
BssAI RCCGGY 3 cut(s) 66, 945, 1422
BssT1I CCWWGG 8 cut(s) 693, 1809, 2535, 2592, 3197, 3760, 4239, 5012
Bst2UI CCWGG 8 cut(s) 591, 2402, 3398, 3752, 4463, 4502, 4551, 5004
Bst6I CTCTTC 5 cut(s) 39, 48, 2924, 4332, 4344
BstAPI GCANNNNNTGC 5 cut(s) 911, 1829, 2066, 3164, 3794
BstAUI TGTACA 1 cut(s) 5272
BstBAI YACGTR 2 cut(s) 3775, 4187
BstBI TTCGAA 2 cut(s) 3487, 3643
BstDSI CCRYGG 4 cut(s) 693, 2535, 2592, 5012
BstENI CCTNNNNNAGG 1 cut(s) 2017
BstF5I GGATG 8 cut(s) 46, 1230, 2378, 2850, 3247, 3368, 4235, 4264
BstFNI CGCG 2 cut(s) 618, 2384
BstHHI GCGC 2 cut(s) 1376, 3357
BstMAI GTCTC 6 cut(s) 1283, 1554, 2165, 3473, 3959, 5238
BstMCI CGRYCG 1 cut(s) 67
BstNI CCWGG 8 cut(s) 591, 2402, 3398, 3752, 4463, 4502, 4551, 5004
BstNSI RCATGY 7 cut(s) 1567, 1775, 1808, 3733, 3788, 4113, 4683
BstSCI CCNGG 9 cut(s) 589, 2400, 2924, 3396, 3750, 4461, 4500, 4549, 5002
BstSFI CTRYAG 5 cut(s) 1743, 1935, 2085, 3654, 4170
BstSLI GKGCMC 1 cut(s) 1106
BstUI CGCG 2 cut(s) 618, 2384
BstV2I GAAGAC 1 cut(s) 2964
BstX2I RGATCY 6 cut(s) 672, 1838, 3061, 3082, 3341, 3586
BstXI CCANNNNNNTGG 1 cut(s) 700
BstYI RGATCY 6 cut(s) 672, 1838, 3061, 3082, 3341, 3586
Bsu15I ATCGAT 2 cut(s) 1083, 4161
BsuI GTATCC 2 cut(s) 3795, 5347
BsuTUI ATCGAT 2 cut(s) 1083, 4161
BtgI CCRYGG 4 cut(s) 693, 2535, 2592, 5012
BtsCI GGATG 8 cut(s) 46, 1230, 2378, 2850, 3247, 3368, 4235, 4264
BtsI GCAGTG 4 cut(s) 992, 1140, 1338, 4167
BveI ACCTGC 3 cut(s) 3189, 4870, 5182
CaiI CAGNNNCTG 1 cut(s) 2830
CfoI GCGC 2 cut(s) 1376, 3357
Cfr10I RCCGGY 3 cut(s) 66, 945, 1422
Cfr13I GGNCC 9 cut(s) 703, 881, 1102, 1103, 2197, 3186, 3307, 3400, 3757
ClaI ATCGAT 2 cut(s) 1083, 4161
CseI GACGC 2 cut(s) 1253, 3902
Csp6I GTAC 7 cut(s) 2679, 3015, 3217, 4118, 5273, 5286, 5302
CspAI ACCGGT 1 cut(s) 66
CviQI GTAC 7 cut(s) 2679, 3015, 3217, 4118, 5273, 5286, 5302
DraI TTTAAA 2 cut(s) 1069, 4125
DraIII CACNNNGTG 1 cut(s) 5146
DriI GACNNNNNGTC 1 cut(s) 5251
EaeI YGGCCR 3 cut(s) 1589, 2353, 2740
Eam1104I CTCTTC 5 cut(s) 39, 48, 2924, 4332, 4344
Eam1105I GACNNNNNGTC 1 cut(s) 5251
EarI CTCTTC 5 cut(s) 39, 48, 2924, 4332, 4344
EciI GGCGGA 2 cut(s) 20, 157
Ecl136II GAGCTC 1 cut(s) 712
Eco130I CCWWGG 8 cut(s) 693, 1809, 2535, 2592, 3197, 3760, 4239, 5012
Eco147I AGGCCT 2 cut(s) 1488, 4244
Eco24I GRGCYC 2 cut(s) 714, 1106
Eco31I GGTCTC 3 cut(s) 1283, 3959, 5238
Eco32I GATATC 2 cut(s) 2443, 4265
Eco47I GGWCC 5 cut(s) 881, 2197, 3186, 3307, 3757
Eco53kI GAGCTC 1 cut(s) 712
Eco57I CTGAAG 6 cut(s) 1148, 1616, 2976, 3191, 4659, 5193
Eco88I CYCGRG 2 cut(s) 320, 5383
EcoICRI GAGCTC 1 cut(s) 712
EcoNI CCTNNNNNAGG 1 cut(s) 2017
EcoO109I RGGNCCY 1 cut(s) 1103
EcoRI GAATTC 2 cut(s) 1049, 3109
EcoRII CCWGG 8 cut(s) 589, 2400, 3396, 3750, 4461, 4500, 4549, 5002
EcoRV GATATC 2 cut(s) 2443, 4265
EcoT14I CCWWGG 8 cut(s) 693, 1809, 2535, 2592, 3197, 3760, 4239, 5012
EcoT22I ATGCAT 6 cut(s) 1279, 1825, 2875, 4111, 4685, 5171
EcoT38I GRGCYC 2 cut(s) 714, 1106
ErhI CCWWGG 8 cut(s) 693, 1809, 2535, 2592, 3197, 3760, 4239, 5012
FalI AAGNNNNNCTT 4 cut(s) 898, 930, 4938, 4970
FaqI GGGAC 2 cut(s) 1190, 3066
FauI CCCGC 3 cut(s) 86, 594, 1611
FauNDI CATATG 2 cut(s) 1783, 3257
FbaI TGATCA 2 cut(s) 3412, 5226
FokI GGATG 8 cut(s) 53, 1237, 2365, 2857, 3254, 3355, 4222, 4271
FriOI GRGCYC 2 cut(s) 714, 1106
GlaI GCGC 2 cut(s) 1375, 3356
GsaI CCCAGC 1 cut(s) 3801
GsuI CTGGAG 7 cut(s) 822, 885, 1165, 1959, 2423, 3773, 4364
HapII CCGG 7 cut(s) 67, 175, 946, 1423, 2492, 2925, 3403
HgaI GACGC 2 cut(s) 1253, 3902
HhaI GCGC 2 cut(s) 1376, 3357
Hin6I GCGC 2 cut(s) 1374, 3355
HinP1I GCGC 2 cut(s) 1374, 3355
HincII GTYRAC 1 cut(s) 4986
HindII GTYRAC 1 cut(s) 4986
HindIII AAGCTT 5 cut(s) 395, 1628, 2654, 2948, 3671
HpaII CCGG 7 cut(s) 67, 175, 946, 1423, 2492, 2925, 3403
Hpy166II GTNNAC 8 cut(s) 208, 452, 2807, 2908, 3704, 3892, 4367, 4986
Hpy8I GTNNAC 8 cut(s) 208, 452, 2807, 2908, 3704, 3892, 4367, 4986
Hpy99I CGWCG 1 cut(s) 22
HpyCH4IV ACGT 7 cut(s) 454, 525, 1649, 1867, 3774, 4186, 4369
HpySE526I ACGT 7 cut(s) 454, 525, 1649, 1867, 3774, 4186, 4369
HspAI GCGC 2 cut(s) 1374, 3355
Kpn2I TCCGGA 1 cut(s) 174
Ksp22I TGATCA 2 cut(s) 3412, 5226
LguI GCTCTTC 1 cut(s) 4344
MaeII ACGT 7 cut(s) 454, 525, 1649, 1867, 3774, 4186, 4369
MfeI CAATTG 5 cut(s) 600, 3911, 3932, 4590, 5256
MflI RGATCY 6 cut(s) 672, 1838, 3061, 3082, 3341, 3586
MhlI GDGCHC 2 cut(s) 714, 1106
MlsI TGGCCA 2 cut(s) 2355, 2742
MluNI TGGCCA 2 cut(s) 2355, 2742
MlyI GAGTC 9 cut(s) 92, 903, 1172, 1697, 1915, 2261, 2954, 5078, 5255
MmeI TCCRAC 3 cut(s) 126, 133, 183
Mox20I TGGCCA 2 cut(s) 2355, 2742
Mph1103I ATGCAT 6 cut(s) 1279, 1825, 2875, 4111, 4685, 5171
MroI TCCGGA 1 cut(s) 174
MscI TGGCCA 2 cut(s) 2355, 2742
MslI CAYNNNNRTG 7 cut(s) 1259, 2361, 2383, 2597, 3256, 4185, 4931
Msp20I TGGCCA 2 cut(s) 2355, 2742
MspA1I CMGCKG 4 cut(s) 1222, 1610, 1979, 2456
MspI CCGG 7 cut(s) 67, 175, 946, 1423, 2492, 2925, 3403
MspR9I CCNGG 9 cut(s) 591, 2402, 2926, 3398, 3752, 4463, 4502, 4551, 5004
MunI CAATTG 5 cut(s) 600, 3911, 3932, 4590, 5256
Mva1269I GAATGC 5 cut(s) 309, 587, 4926, 5171, 5384
MvaI CCWGG 8 cut(s) 591, 2402, 3398, 3752, 4463, 4502, 4551, 5004
MvnI CGCG 2 cut(s) 618, 2384
NciI CCSGG 1 cut(s) 2926
NcoI CCATGG 4 cut(s) 693, 2535, 2592, 5012
NdeI CATATG 2 cut(s) 1783, 3257
NlaIV GGNNCC 6 cut(s) 705, 1104, 2923, 3918, 4884, 5151
NsiI ATGCAT 6 cut(s) 1279, 1825, 2875, 4111, 4685, 5171
NspI RCATGY 7 cut(s) 1567, 1775, 1808, 3733, 3788, 4113, 4683
NspV TTCGAA 2 cut(s) 3487, 3643
OliI CACNNNNGTG 1 cut(s) 2361
PaeI GCATGC 4 cut(s) 1808, 3733, 3788, 4683
PaeR7I CTCGAG 1 cut(s) 320
PaqCI CACCTGC 1 cut(s) 3189
PceI AGGCCT 2 cut(s) 1488, 4244
PciI ACATGT 1 cut(s) 1563
PciSI GCTCTTC 1 cut(s) 4344
PcsI WCGNNNNNNNCGW 1 cut(s) 531
PctI GAATGC 5 cut(s) 309, 587, 4926, 5171, 5384
PfeI GAWTC 9 cut(s) 509, 818, 887, 1157, 1368, 2509, 3278, 4496, 4546
PflMI CCANNNNNTGG 1 cut(s) 3583
PfoI TCCNGGA 1 cut(s) 2924
PinAI ACCGGT 1 cut(s) 66
PleI GAGTC 9 cut(s) 92, 902, 1172, 1696, 1914, 2260, 2954, 5078, 5255
PpsI GAGTC 9 cut(s) 92, 902, 1172, 1696, 1914, 2260, 2954, 5078, 5255
Ppu21I YACGTR 2 cut(s) 3775, 4187
PscI ACATGT 1 cut(s) 1563
PsiI TTATAA 2 cut(s) 437, 3072
Psp124BI GAGCTC 1 cut(s) 714
Psp1406I AACGTT 1 cut(s) 525
Psp6I CCWGG 8 cut(s) 589, 2400, 3396, 3750, 4461, 4500, 4549, 5002
PspFI CCCAGC 1 cut(s) 3797
PspGI CCWGG 8 cut(s) 589, 2400, 3396, 3750, 4461, 4500, 4549, 5002
PspN4I GGNNCC 6 cut(s) 705, 1104, 2923, 3918, 4884, 5151
PspOMI GGGCCC 1 cut(s) 1102
PspPI GGNCC 9 cut(s) 703, 881, 1102, 1103, 2197, 3186, 3307, 3400, 3757
PstI CTGCAG 3 cut(s) 1939, 2089, 4174
PstNI CAGNNNCTG 1 cut(s) 2830
PsuI RGATCY 6 cut(s) 672, 1838, 3061, 3082, 3341, 3586
PvuII CAGCTG 2 cut(s) 1610, 1979
RsaI GTAC 7 cut(s) 2680, 3016, 3218, 4119, 5274, 5287, 5303
RsaNI GTAC 7 cut(s) 2679, 3015, 3217, 4118, 5273, 5286, 5302
RseI CAYNNNNRTG 7 cut(s) 1259, 2361, 2383, 2597, 3256, 4185, 4931
SacI GAGCTC 1 cut(s) 714
SapI GCTCTTC 1 cut(s) 4344
Sau96I GGNCC 9 cut(s) 703, 881, 1102, 1103, 2197, 3186, 3307, 3400, 3757
ScaI AGTACT 1 cut(s) 4119
SchI GAGTC 9 cut(s) 92, 903, 1172, 1697, 1915, 2261, 2954, 5078, 5255
ScrFI CCNGG 9 cut(s) 591, 2402, 2926, 3398, 3752, 4463, 4502, 4551, 5004
SduI GDGCHC 2 cut(s) 714, 1106
SfcI CTRYAG 5 cut(s) 1743, 1935, 2085, 3654, 4170
Sfr274I CTCGAG 1 cut(s) 320
SfuI TTCGAA 2 cut(s) 3487, 3643
SinI GGWCC 5 cut(s) 881, 2197, 3186, 3307, 3757
SlaI CTCGAG 1 cut(s) 320
SmiMI CAYNNNNRTG 7 cut(s) 1259, 2361, 2383, 2597, 3256, 4185, 4931
SmlI CTYRAG 6 cut(s) 320, 513, 606, 4200, 4297, 5154
SmoI CTYRAG 6 cut(s) 320, 513, 606, 4200, 4297, 5154
SpeI ACTAGT 1 cut(s) 3994
SphI GCATGC 4 cut(s) 1808, 3733, 3788, 4683
SseBI AGGCCT 2 cut(s) 1488, 4244
SspI AATATT 2 cut(s) 1298, 2395
SstI GAGCTC 1 cut(s) 714
StuI AGGCCT 2 cut(s) 1488, 4244
StyD4I CCNGG 9 cut(s) 589, 2400, 2924, 3396, 3750, 4461, 4500, 4549, 5002
StyI CCWWGG 8 cut(s) 693, 1809, 2535, 2592, 3197, 3760, 4239, 5012
TaiI ACGT 7 cut(s) 457, 528, 1652, 1870, 3777, 4189, 4372
TaqII GACCGA 3 cut(s) 3930, 4206, 5241
TatI WGTACW 4 cut(s) 2678, 3014, 4117, 5272
TauI GCSGC 2 cut(s) 962, 2456
TfiI GAWTC 9 cut(s) 509, 818, 887, 1157, 1368, 2509, 3278, 4496, 4546
TspGWI ACGGA 4 cut(s) 80, 1954, 2070, 2953
Van91I CCANNNNNTGG 1 cut(s) 3583
VpaK11BI GGWCC 5 cut(s) 881, 2197, 3186, 3307, 3757
XagI CCTNNNNNAGG 1 cut(s) 2017
XapI RAATTY 9 cut(s) 361, 536, 1049, 1327, 1667, 2775, 3109, 3490, 3831
XbaI TCTAGA 2 cut(s) 890, 2099
XceI RCATGY 7 cut(s) 1567, 1775, 1808, 3733, 3788, 4113, 4683
XcmI CCANNNNNNNNNTGG 1 cut(s) 379
XhoI CTCGAG 1 cut(s) 320
ZrmI AGTACT 1 cut(s) 4119
Zsp2I ATGCAT 6 cut(s) 1279, 1825, 2875, 4111, 4685, 5171
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.