MD03G1157000.v1.1

Proteasome-associated protein ECM29 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0006358
Physical Location & Seq
Forward (+)
18608181 .. 18642074
33894 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1157000.v1.1.491

Sequence Viewer

Length: 5466 bp
ATGGCGGAAGCATCATCGGCGTCTGCGACAAAGTCGGATGAAGAGAAAGTAGAGATGCTGGACCGGTTGCTAACTCGGCTAGCTCTCTGCGATGACTCCAACCTCCAACCCTTACTCTCCAAGCTTCTCCCCTTCACCATTTCCTCACTTTCTTCTCAATCCTCCGCCGTCCGCAACAAGGTTCTTGAGATATTGAGTCACGTAAACAAGAGAGTGAAGCACCAACCTGAGATTGGTTTACCCTTGTCGGAGTTATGGAATATATATTCGGAAGCTAATACTGCTTCAATGGTCAGGAATTTCTGCATTCTGTATATCGAGATGGCAATGGACCGTGCAGATACAAAACAAAAGGAAGATTTGGCAGCCATACTTCTGTCGGGTGTTTCCAGGCTTCCTCTTCAGCACCAAGAAATTATACTCAGACTCGCTACTAAGGTCATTGGTGAATGTCACTCAAAAGGAATTAACAATGAAGTTGCTGCAAAGTATAGAATGATAAGTGATTCCCAGGACAGGAACGTGTTTCTTGAATTTTGCCTTCATACATTGTTGTATCAACAGTCATCTCAAAGAGAATGCCCACCTGGACTTTCGGTTGCTCAAACCAACCGTGTGACAGGGAAACAACCATTGAAAAATGATATACTTCTAACAAGAAAGTTAGGGATCTTGAATGTTATTGACGCTATGGAGCTGGCCCCTGAGCTCGTTTATCCGCTATACGTGGCTTCCTCCGTAGATTGTCAAGAGCCTGTTGTTAAGAGAGGGGAGGAGCTTCTAAAGAAGAAGGCTGCTGGAGCTAACTTAGATGATTCAGACCTGATAAACAAACTTTTTTTGTTATTTAATGGTACTGCTGGATCTCAAAGTGTTGCTCCTGAATCTAGAATCACTCCGGCAAATCCTGCTTTGAAAGCAAAACTTATATCTATTTTCTGCCGATCTATTACAACAGCCAACAGTTTCCCTGCAACTTTACAGTGCATTTTTGGTTGCATATATGGAAGTGATACCACATCGAGGTTGAAGCAGTTGGGAATGGAATTCACTGTCTGGGTATTTAAACATTCAAAGATTGATCAACTCAAACTCATGGGCCCTGTTATTCTGAGTGGAATTCTGAAGTCACTTGACACTGTTTCAAGTTCAGAATCAGATGCTACTGTTAGGGACTCCAAAACTTTTGCTTATCAAGCAATTGGGTTGCTTGCACAGCGCATGCCTCAACTTTTCAGAGATAAGATTGACATGGCTGTGCGTCTTTTTGATGCATTGAAGGTGGAGGCCCAGCATTTTCGCCTCAGTATACAAGAAGCAACCAATTCCCTTGCCACTGCTTACAAGGGTGCCCCATCAACAGTGCTAAAGGATCTGGAGATGCTTTTGCTAAAAAATGCTCAAGAGGAACAAAGTGAAGTACGATTCTGTGTGATAAGATGGGCGACTTCTTTATTTGATTTGCAACATTGCCCTAGCCGATTTATTTGTATGCTTGGGGCAGCTGATACTAAATTGGATATAAGGGAGATAGCTCTTGAGGGCCTTTTACTTGTGAAAGATGATGGGCAAATCATGAGTCAGAAGCAGGAGCTACATTACCCAAAACTTGGAGTCATGTTAGATTATATTCTTAGGCAGCAACCGAACTTATTAGATTCATCTGAGATGAGGGAGCAGAAGCTTCATTTTCCCTCAAAAACATATCTAGTTATGATTCAATTTCTGTTGAAGTGCTTCGACTCGGAATTGGAGCAAGACAGTTCTATTAAAGGGTCAACTGATTTTCAGTCTTCTGTAGAAGCACTCTGCTTACTTCTTGAACATGCTATGGCCTTTGAAGGGTCTGTCGAGTTGCATGCCCAGGCTTCCAAGGCTTTAATTGTAATTGGATCTTGTATGCCAGAGCTGATAGCCTCGCGCTATGCCCAAAAAGTTTCATGGCTAAGGCAACTACTCAGTCATGTTGACTTGGATACCCGTGAAGCTGCAGCACGTTTACTTGGGTTTGCTTCCTCTGCTCTTGCTCCAGCTGAATCTTCTGCTCTTATCTCTGAACTAATTGCCTCAGTTAGTGGAAGACGTAAGATAAGGTTTGAAGAACAACATGGAGCATTATGTGCAGTAGGATATGTAACTGCAGACTGTATGTCGCGAACACCAGCCATTCCAGATGCACTGTTTCAAACTACATTAAAGTGCCTTGTTGATGTTACTAATTCTGAGACTGCACCACTGGCCTCAATAGCAACCCAAGCCTTAGGTCACATTGGGCTGATTATCCCATTGCCTTCGCTTGTTATTGATTCTAGTTCAGTTGATATTCTGGTTGTTTTAAATGAGAAGTTGAGAAAACTACTCGGTGGCGATGACAATAAAGCAATTCAAAAAGTTGTTATATCCATTGGCCATATGTGTGTAAAGGAAACTTCATCTTCACGTCTAAATATTGCCCTCGATTTAATTTTCAGTCTTTCCCGATCCAAGGTCGAGGACATCCTCTTTGCTGCTGGCGAGGCTCTATCATTTTTATGGGGTGGTGTTCCAGTGACTGCAGATTTAATTCTCAAAGCTAATTATTCGCTTTCGATGGCTTCGAACTTTCTTATGGGAGATGTAACCTCATCTCTGTCAAAATATATCTCAATTGAAAAGAATGGAGCTGAAGAAGAACGTAATGCCAAAGTTAGAGATGCAATAACTAAAAAGCTCTTTGACGATCTTCTATACAGTACAAGAAAGGAAGATCGGTGTTCTGGAACTGTTTGGTTATTATCAATTACAATGTATTGTGGCCATGACCCAGCTATCCAGAAAATGCTTCCAGACATTCAGGAGGCTTTTTCACATCTTCTGGGCGAACAGAATGAACTTACACAGGAGCTGGCCTCACAAGGGATGAGCATTGTGTATGAACTTGGTGATGCAGCAATGAAGGAGAATTTGGTCCATGCACTTGTTAACTCTCTGACTGGTTCAGGGAAGAGGAAAAGAGCTATCAAGCTCGATGAAGACTCTGAAGTGTTTCAAGAGGGAGTTATTGGTGAAGGCCTGAGTGGAGGGAAACTGAGCACATACAAGGAGCTTTGCAATGTTGCAAATGAGATGGGGCAACCAGATCTAATTTATAAGTTTATGGATCTGGCTAATTATCAAGCCTCTCTAAATTCCAAGAGGGGTGCTGCCTTTGGATTTTCAAAGATAGCTAAGCAAGCAGGAGATGCTCTAAAGCCACACTTGCGTTCATTGATTCCAAGACTAGTTCGGTACCAATACGATCCTGACAAAAATGTGCAGGATGCTATGTCACACATATGGAAATCACTAGTAGCAGATTCAAAGAAAACCATTGATGAGAACATGGACCTCATTGTTGATGATTTGTTAATACAATGTGGATCCCGGCTTTGGCGCTCCCGTGAGTCGTCATGTCTTGCTCTTGCGGATATCATTCAGGGTCGAAAGTTTGATCAGGTGGGGAAGCATTTAAGAAAATTATGGTCAGCAGCGTTCCGTGCCATGGATGACATAAAAGAGACTGTTCGAAACTCTGGTGACAAGTTATGCCGTGCTTTAACTTCATTGACAGTGAGGCTTTCTGATGTCTCCCTAACTGATGTATCAGAGGCACGCCATACAATGGATATTGTTCTGCCTTATCTGCTTACAGATGGAATACTAAGTAAAGTTGATAGTATTCGCAAAGCATCTATCGGAGTTGTTATGAAGCTTGCAAAGGGTGCAGGGATTGCAATTCGCCCACATTTATCTGATTTGGTTTGTTGCATGCTGGAAAGTTTATCTAGCCTTGAGGACCAAGGGCTTAACTATGTGGAGTTGCATGCAGCAAATGCTGGAATACAGACAGAGAAGCTTGAGAACTTAAGAATTTCAATTGCAAAAGGTTCTCCTATGTGGGAAACTCTTGATCTCTGCATTAAAGTTGTGGATGTGGAATCATTAGATCAATTGGTTCCTCGTCTTGCTCAGTTGGTTCGGTCCGGAGTGGGCCTCAATACAAGGGTTGGGGTTGCCAGCTTTATTACATTACTAGTTCAAAAAGTTGGTGTTGAAATCAAACCATATACGAGCAGGCTACTGAGGCTGTTGTTTCCAGTTGTTAAGGATGAAAAAAGTGCTGCATCAAAACGTGCCTTTGCTGGTGCTTGTGCAGCAGTTTTAAAGCATGCAGCACCTACTCAGGCAGAAAAATTGATTGACGATACTGTAGCTTTGCATACCGGTGATAAAAATTATCAAGTTTCATGTGCAATTTTATTGAAAAGCTATTCATCTGTGGCCTCAGATGCCTTGAGTGGATATCTTGCAGCAATTATTCCAGCTATTTTTATCTCAAGATTTGAGGATGACAAAGTTATTTCTAGCCTGTTTGAAGAGTTGTGGGAAGAGCATACAAGTGGTGAAAGGGTAGCCCTTCAGTTGTACTTAGTAGAAATTGTTTCTTTGATTTGTGAGGGCATTGGATCATCATCATGGGCAAGTAAAAAGAAGTCAGCACAGGCAATCTCCAAGCTCAGTGATGTTTTGGGTGAATCGTTGTCTTCTTACTATCCTGTCTTGCTCCAATCTCTTATGAAAGAGATTCCTGGTCGGTTATGGGAGGGAAAGGATGCTCTATTAGATGCAATAGCTGCTCTTTCTGTGTCTTGCCACAAAGCAATTTCAAGTGATGATCCCTCCACCCTGAATGAAATTTTGAGCATAGTATCTTCTGCATGCACCAAAAAAGCAAAGAAATACCGTGAAGCAGCTCTCACCTGTCTTGAAAAGGTTGTTAGAGCCTTTGGTAACCAAGAGGTCTTTAATGTGGTTTTTCCATTATTACTTGAGATTTTCAGTTCTGCCAGTCTCGATCAGTCTGGGAAAGAATCTTTGGCTGGTGATGCTGCCAAAGCAGAAGATCAAGTTGAAAAGGTTTCTGTTCCACACAACAAAATTTTGGACTGCATGACAGCCTGCATCCATGTGGCACATATAAATGATATTCTTGGACAACAGAAGAACTTCTTGCATGTGTTGATTACCACGCTGTCATCTGGACTTCCATGGACAGTCAAAGTATCTGCACTTTCATCAACGAAGGAACTTTGTTCAATGCTTCAGAAGGCTCTGGATGACTCTCAAGAACCTTCTATACATGCCAGCATAATATCTTCAGTTCAGGAGTTGTTTCTCTCAATGCCACCTCAAATAGTTGAATGCATACGAACAATTAACGTTGCCCAGGTTCACGTCGCTGCTTCAGAGACCCTTCTTGTGATCGTCAAGCTGTATGAGAAGCTCCAAACGATACATTGTAGGGACGAAAAATTTATGGGCGAGCTTGTTCATCTGTATGAGGTGGAGAAAAATGGTGAAGCAAAGTCCTTGTTGAAAAAATGTGTCGACGCTCTTGAAAACCTGAAACAAGAGGATGTCGAAGATGACTCGATGTGTTCGGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ecm29

Protein Analysis

1822

Amino Acids

200.1

Weight (kDa)

5.98

Isoelectric Point (pI)

41.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ECM29_N PF13001 20 - 500 9.3e-135 Proteasome component ECM29, N-terminal
ARM_ECM29 PF23702 626 - 720 4e-11 ECM29, ARM-like repeats
HEAT_GCN1 PF23271 1066 - 1214 5.1e-07 Stalled ribosome sensor GCN1-like, HEAT repeats
HEAT_ECM29 PF24492 1252 - 1411 1.8e-48 Proteasome adapter and scaffold protein ECM29, HEAT-repeat domain
ARM_ECM29_C PF23731 1558 - 1802 1.1e-08 ECM29, C-terminal ARM-like repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3129
Acc65I GGTACC 1 cut(s) 3267
AccB1I GGYRCC 2 cut(s) 1349, 3267
AccB7I CCANNNNNTGG 2 cut(s) 1610, 3640
AccI GTMKAC 2 cut(s) 1309, 5406
AccII CGCG 2 cut(s) 1921, 2155
AccIII TCCGGA 1 cut(s) 4001
AciI CCGC 5 cut(s) 5, 165, 172, 719, 3443
AclI AACGTT 1 cut(s) 5238
AcoI YGGCCR 2 cut(s) 2407, 2794
AcuI CTGAAG 8 cut(s) 386, 1145, 2685, 3039, 4421, 5105, 5160, 5247
AcyI GRCGYC 1 cut(s) 20
AfaI GTAC 5 cut(s) 856, 1422, 2734, 3269, 4445
AflII CTTAAG 1 cut(s) 3883
AflIII ACRYGT 1 cut(s) 522
AgeI ACCGGT 2 cut(s) 63, 4241
AhdI GACNNNNNGTC 1 cut(s) 2149
AhlI ACTAGT 3 cut(s) 3259, 3325, 4051
AjiI CACGTC 2 cut(s) 2441, 5254
AjnI CCWGG 6 cut(s) 389, 510, 586, 1863, 4606, 5244
AjuI GAANNNNNNNTTGG 4 cut(s) 2927, 2959, 4877, 4909
Alw21I GWGCWC 2 cut(s) 711, 3074
Alw26I GTCTC 5 cut(s) 2219, 3530, 3610, 4874, 5261
AlwNI CAGNNNCTG 2 cut(s) 2552, 2884
Aor13HI TCCGGA 1 cut(s) 4001
ApaI GGGCCC 1 cut(s) 1103
ArsI GACNNNNNNTTYG 4 cut(s) 1564, 1596, 2486, 2518
AsiGI ACCGGT 2 cut(s) 63, 4241
Asp700I GAANNNNTTC 3 cut(s) 1736, 3024, 5024
Asp718I GGTACC 1 cut(s) 3267
AspLEI GCGC 3 cut(s) 1221, 1923, 3414
AsuC2I CCSGG 1 cut(s) 3403
AsuII TTCGAA 2 cut(s) 2597, 3544
AsuNHI GCTAGC 1 cut(s) 79
AvaII GGWCC 6 cut(s) 61, 331, 2947, 3364, 3814, 3999
AxyI CCTNAGG 1 cut(s) 2260
BaeGI GKGCMC 2 cut(s) 1103, 1354
BaeI ACNNNNGTAYC 2 cut(s) 3603, 3636
BalI TGGCCA 2 cut(s) 2409, 2796
BamHI GGATCC 1 cut(s) 3398
BanI GGYRCC 2 cut(s) 1349, 3267
BanII GRGCYC 2 cut(s) 711, 1103
BbsI GAAGAC 4 cut(s) 1785, 2086, 3018, 4554
Bbv12I GWGCWC 2 cut(s) 711, 3074
BccI CCATC 7 cut(s) 316, 1363, 1434, 1559, 2584, 3100, 3665
BceAI ACGGC 2 cut(s) 152, 3552
BcgI CGANNNNNNTGC 2 cut(s) 1841, 1875
BciT130I CCWGG 6 cut(s) 391, 512, 588, 1865, 4608, 5246
BciVI GTATCC 1 cut(s) 1969
BclI TGATCA 2 cut(s) 1081, 3469
BcnI CCSGG 1 cut(s) 3403
BcoDI GTCTC 5 cut(s) 2219, 3530, 3610, 4874, 5261
BcuI ACTAGT 3 cut(s) 3259, 3325, 4051
BfmI CTRYAG 5 cut(s) 1797, 1989, 2139, 2553, 4227
BfoI RGCGCY 1 cut(s) 3415
BfrI CTTAAG 1 cut(s) 3883
BfuI GTATCC 1 cut(s) 1969
BglII AGATCT 1 cut(s) 3118
BlpI GCTNAGC 1 cut(s) 3207
Bme1390I CCNGG 7 cut(s) 391, 512, 588, 1865, 3403, 4608, 5246
Bme18I GGWCC 6 cut(s) 61, 331, 2947, 3364, 3814, 3999
BmeRI GACNNNNNGTC 1 cut(s) 2149
BmgBI CACGTC 2 cut(s) 2441, 5254
BmiI GGNNCC 6 cut(s) 702, 1101, 1351, 3269, 3400, 3975
BmrFI CCNGG 7 cut(s) 391, 512, 588, 1865, 3403, 4608, 5246
BmtI GCTAGC 1 cut(s) 83
BpiI GAAGAC 4 cut(s) 1785, 2086, 3018, 4554
BplI GAGNNNNNCTC 4 cut(s) 2873, 2905, 3996, 4028
BpmI CTGGAG 3 cut(s) 819, 1397, 2013
Bpu10I CCTNAGC 2 cut(s) 705, 1946
Bpu1102I GCTNAGC 1 cut(s) 3207
Bpu14I TTCGAA 2 cut(s) 2597, 3544
BpuEI CTTGAG 9 cut(s) 206, 1386, 1559, 3830, 3896, 4333, 4339, 4868, 5127
BpuMI CCSGG 1 cut(s) 3403
BsaAI YACGTR 2 cut(s) 202, 727
BsaBI GATNNNNATC 1 cut(s) 4489
BsaHI GRCGYC 1 cut(s) 20
BsaI GGTCTC 1 cut(s) 5261
BsaJI CCNNGG 8 cut(s) 510, 1863, 1872, 2484, 3519, 3817, 5066, 5244
BsaWI WCCGGW 3 cut(s) 63, 4001, 4241
Bse118I RCCGGY 2 cut(s) 63, 4241
Bse1I ACTGG 5 cut(s) 2241, 2546, 2977, 4115, 4866
Bse21I CCTNAGG 1 cut(s) 2260
Bse3DI GCAATG 5 cut(s) 333, 1468, 2285, 2937, 3097
Bse8I GATNNNNATC 1 cut(s) 4489
BseAI TCCGGA 1 cut(s) 4001
BseBI CCWGG 6 cut(s) 391, 512, 588, 1865, 4608, 5246
BseDI CCNNGG 8 cut(s) 510, 1863, 1872, 2484, 3519, 3817, 5066, 5244
BseJI GATNNNNATC 1 cut(s) 4489
BseMI GCAATG 5 cut(s) 333, 1468, 2285, 2937, 3097
BseNI ACTGG 5 cut(s) 2241, 2546, 2977, 4115, 4866
BseRI GAGGAG 1 cut(s) 788
BseSI GKGCMC 2 cut(s) 1103, 1354
BseYI CCCAGC 2 cut(s) 1290, 2803
BsgI GTGCAG 7 cut(s) 357, 2142, 2214, 3314, 3762, 4191, 5070
Bsh1236I CGCG 2 cut(s) 1921, 2155
BshNI GGYRCC 2 cut(s) 1349, 3267
BshTI ACCGGT 2 cut(s) 63, 4241
BsiHKAI GWGCWC 2 cut(s) 711, 3074
BsiSI CCGG 5 cut(s) 64, 899, 3403, 4002, 4242
BslFI GGGAC 2 cut(s) 1187, 5336
BsmAI GTCTC 5 cut(s) 2219, 3530, 3610, 4874, 5261
BsmFI GGGAC 2 cut(s) 1187, 5336
BsmI GAATGC 3 cut(s) 306, 584, 5225
Bso31I GGTCTC 1 cut(s) 5261
Bsp119I TTCGAA 2 cut(s) 2597, 3544
Bsp120I GGGCCC 1 cut(s) 1099
Bsp1286I GDGCHC 4 cut(s) 711, 1103, 1354, 3074
Bsp13I TCCGGA 1 cut(s) 4001
Bsp1720I GCTNAGC 1 cut(s) 3207
Bsp19I CCATGG 2 cut(s) 3519, 5066
Bsp68I TCGCGA 1 cut(s) 2155
BspACI CCGC 5 cut(s) 5, 165, 172, 719, 3443
BspEI TCCGGA 1 cut(s) 4001
BspFNI CGCG 2 cut(s) 1921, 2155
BspHI TCATGA 1 cut(s) 1575
BspLI GGNNCC 6 cut(s) 702, 1101, 1351, 3269, 3400, 3975
BspMAI CTGCAG 3 cut(s) 1993, 2143, 2557
BspOI GCTAGC 1 cut(s) 83
BspQI GCTCTTC 1 cut(s) 4401
BspT104I TTCGAA 2 cut(s) 2597, 3544
BspT107I GGYRCC 2 cut(s) 1349, 3267
BspTI CTTAAG 1 cut(s) 3883
BspTNI GGTCTC 1 cut(s) 5261
BsrDI GCAATG 5 cut(s) 333, 1468, 2285, 2937, 3097
BsrFI RCCGGY 2 cut(s) 63, 4241
BsrI ACTGG 5 cut(s) 2241, 2546, 2977, 4115, 4866
BssAI RCCGGY 2 cut(s) 63, 4241
BssECI CCNNGG 8 cut(s) 510, 1863, 1872, 2484, 3519, 3817, 5066, 5244
BssNAI GTATAC 1 cut(s) 1310
BssNI GRCGYC 1 cut(s) 20
BssT1I CCWWGG 5 cut(s) 1872, 2484, 3519, 3817, 5066
Bst1107I GTATAC 1 cut(s) 1310
Bst2UI CCWGG 6 cut(s) 391, 512, 588, 1865, 4608, 5246
Bst6I CTCTTC 5 cut(s) 36, 405, 2978, 4389, 4401
BstACI GRCGYC 1 cut(s) 20
BstAFI CTTAAG 1 cut(s) 3883
BstAPI GCANNNNNTGC 7 cut(s) 908, 2120, 3221, 3740, 3749, 3851, 4652
BstBAI YACGTR 2 cut(s) 202, 727
BstBI TTCGAA 2 cut(s) 2597, 3544
BstDSI CCRYGG 2 cut(s) 3519, 5066
BstEII GGTNACC 1 cut(s) 4808
BstFNI CGCG 2 cut(s) 1921, 2155
BstH2I RGCGCY 1 cut(s) 3415
BstHHI GCGC 3 cut(s) 1221, 1923, 3414
BstMAI GTCTC 5 cut(s) 2219, 3530, 3610, 4874, 5261
BstNI CCWGG 6 cut(s) 391, 512, 588, 1865, 4608, 5246
BstNSI RCATGY 9 cut(s) 1225, 1829, 1862, 3790, 3845, 4190, 4740, 5036, 5162
BstPI GGTNACC 1 cut(s) 4808
BstSCI CCNGG 7 cut(s) 389, 510, 586, 1863, 3401, 4606, 5244
BstSFI CTRYAG 5 cut(s) 1797, 1989, 2139, 2553, 4227
BstSLI GKGCMC 2 cut(s) 1103, 1354
BstUI CGCG 2 cut(s) 1921, 2155
BstV2I GAAGAC 4 cut(s) 1785, 2086, 3018, 4554
BstX2I RGATCY 7 cut(s) 669, 863, 1372, 1892, 3118, 3139, 3398
BstYI RGATCY 7 cut(s) 669, 863, 1372, 1892, 3118, 3139, 3398
BstZ17I GTATAC 1 cut(s) 1310
Bsu36I CCTNAGG 1 cut(s) 2260
BsuI GTATCC 1 cut(s) 1969
BtgI CCRYGG 2 cut(s) 3519, 5066
BtgZI GCGATG 2 cut(s) 105, 2382
BtrI CACGTC 2 cut(s) 2441, 5254
BtsI GCAGTG 1 cut(s) 1335
BtuMI TCGCGA 1 cut(s) 2155
CaiI CAGNNNCTG 2 cut(s) 2552, 2884
CciI TCATGA 1 cut(s) 1575
CfoI GCGC 3 cut(s) 1221, 1923, 3414
Cfr10I RCCGGY 2 cut(s) 63, 4241
CpoI CGGWCCG 1 cut(s) 3999
CseI GACGC 4 cut(s) 9, 695, 1250, 5417
Csp6I GTAC 5 cut(s) 855, 1421, 2733, 3268, 4444
CspAI ACCGGT 2 cut(s) 63, 4241
CspI CGGWCCG 1 cut(s) 3999
CviQI GTAC 5 cut(s) 855, 1421, 2733, 3268, 4444
DraI TTTAAA 3 cut(s) 1066, 2337, 4182
DriI GACNNNNNGTC 1 cut(s) 2149
EaeI YGGCCR 2 cut(s) 2407, 2794
Eam1104I CTCTTC 5 cut(s) 36, 405, 2978, 4389, 4401
Eam1105I GACNNNNNGTC 1 cut(s) 2149
EarI CTCTTC 5 cut(s) 36, 405, 2978, 4389, 4401
EciI GGCGGA 2 cut(s) 20, 154
Ecl136II GAGCTC 1 cut(s) 709
Eco130I CCWWGG 5 cut(s) 1872, 2484, 3519, 3817, 5066
Eco147I AGGCCT 1 cut(s) 3051
Eco24I GRGCYC 2 cut(s) 711, 1103
Eco31I GGTCTC 1 cut(s) 5261
Eco32I GATATC 2 cut(s) 3448, 4322
Eco47I GGWCC 6 cut(s) 61, 331, 2947, 3364, 3814, 3999
Eco53kI GAGCTC 1 cut(s) 709
Eco57I CTGAAG 8 cut(s) 386, 1145, 2685, 3039, 4421, 5105, 5160, 5247
Eco81I CCTNAGG 1 cut(s) 2260
Eco91I GGTNACC 1 cut(s) 4808
EcoICRI GAGCTC 1 cut(s) 709
EcoO109I RGGNCCY 2 cut(s) 1100, 1543
EcoO65I GGTNACC 1 cut(s) 4808
EcoRI GAATTC 2 cut(s) 1046, 1119
EcoRII CCWGG 6 cut(s) 389, 510, 586, 1863, 4606, 5244
EcoRV GATATC 2 cut(s) 3448, 4322
EcoT14I CCWWGG 5 cut(s) 1872, 2484, 3519, 3817, 5066
EcoT22I ATGCAT 2 cut(s) 1276, 5225
EcoT38I GRGCYC 2 cut(s) 711, 1103
ErhI CCWWGG 5 cut(s) 1872, 2484, 3519, 3817, 5066
FalI AAGNNNNNCTT 6 cut(s) 909, 941, 3221, 3253, 5012, 5044
FaqI GGGAC 2 cut(s) 1187, 5336
FauNDI CATATG 2 cut(s) 2412, 3314
FbaI TGATCA 2 cut(s) 1081, 3469
FblI GTMKAC 2 cut(s) 1309, 5406
FriOI GRGCYC 2 cut(s) 711, 1103
GlaI GCGC 3 cut(s) 1220, 1922, 3413
GsaI CCCAGC 2 cut(s) 1294, 2807
GsuI CTGGAG 3 cut(s) 819, 1397, 2013
HaeII RGCGCY 1 cut(s) 3415
HapII CCGG 5 cut(s) 64, 899, 3403, 4002, 4242
HgaI GACGC 4 cut(s) 9, 695, 1250, 5417
HhaI GCGC 3 cut(s) 1221, 1923, 3414
Hin1I GRCGYC 1 cut(s) 20
Hin6I GCGC 3 cut(s) 1219, 1921, 3412
HinP1I GCGC 3 cut(s) 1219, 1921, 3412
HincII GTYRAC 4 cut(s) 1779, 1969, 2962, 5407
HindII GTYRAC 4 cut(s) 1779, 1969, 2962, 5407
HindIII AAGCTT 4 cut(s) 122, 1682, 3728, 3872
HpaI GTTAAC 1 cut(s) 2962
HpaII CCGG 5 cut(s) 64, 899, 3403, 4002, 4242
Hpy166II GTNNAC 9 cut(s) 205, 239, 1310, 1779, 1969, 2000, 2962, 5251, 5407
Hpy8I GTNNAC 9 cut(s) 205, 239, 1310, 1779, 1969, 2000, 2962, 5251, 5407
Hpy99I CGWCG 2 cut(s) 5258, 5411
Hsp92I GRCGYC 1 cut(s) 20
HspAI GCGC 3 cut(s) 1219, 1921, 3412
Kpn2I TCCGGA 1 cut(s) 4001
KpnI GGTACC 1 cut(s) 3271
Ksp22I TGATCA 2 cut(s) 1081, 3469
KspAI GTTAAC 1 cut(s) 2962
LguI GCTCTTC 1 cut(s) 4401
MfeI CAATTG 4 cut(s) 1200, 2646, 3894, 3968
MflI RGATCY 7 cut(s) 669, 863, 1372, 1892, 3118, 3139, 3398
MhlI GDGCHC 4 cut(s) 711, 1103, 1354, 3074
MlsI TGGCCA 2 cut(s) 2409, 2796
MluNI TGGCCA 2 cut(s) 2409, 2796
MmeI TCCRAC 4 cut(s) 15, 123, 130, 228
Mox20I TGGCCA 2 cut(s) 2409, 2796
Mph1103I ATGCAT 2 cut(s) 1276, 5225
MroI TCCGGA 1 cut(s) 4001
MroXI GAANNNNTTC 3 cut(s) 1736, 3024, 5024
MscI TGGCCA 2 cut(s) 2409, 2796
Msp20I TGGCCA 2 cut(s) 2409, 2796
MspA1I CMGCKG 2 cut(s) 1505, 2033
MspCI CTTAAG 1 cut(s) 3883
MspI CCGG 5 cut(s) 64, 899, 3403, 4002, 4242
MspR9I CCNGG 7 cut(s) 391, 512, 588, 1865, 3403, 4608, 5246
MunI CAATTG 4 cut(s) 1200, 2646, 3894, 3968
Mva1269I GAATGC 3 cut(s) 306, 584, 5225
MvaI CCWGG 6 cut(s) 391, 512, 588, 1865, 4608, 5246
MvnI CGCG 2 cut(s) 1921, 2155
NciI CCSGG 1 cut(s) 3403
NcoI CCATGG 2 cut(s) 3519, 5066
NdeI CATATG 2 cut(s) 2412, 3314
NheI GCTAGC 1 cut(s) 79
NlaIV GGNNCC 6 cut(s) 702, 1101, 1351, 3269, 3400, 3975
NmeAIII GCCGAG 1 cut(s) 55
NmuCI GTSAC 8 cut(s) 197, 452, 616, 1128, 2264, 2548, 3306, 3554
NruI TCGCGA 1 cut(s) 2155
NsiI ATGCAT 2 cut(s) 1276, 5225
NspI RCATGY 9 cut(s) 1225, 1829, 1862, 3790, 3845, 4190, 4740, 5036, 5162
NspV TTCGAA 2 cut(s) 2597, 3544
PaeI GCATGC 6 cut(s) 1225, 1862, 3790, 3845, 4190, 4740
PagI TCATGA 1 cut(s) 1575
PceI AGGCCT 1 cut(s) 3051
PciSI GCTCTTC 1 cut(s) 4401
PcsI WCGNNNNNNNCGW 2 cut(s) 23, 2594
PctI GAATGC 3 cut(s) 306, 584, 5225
PdmI GAANNNNTTC 3 cut(s) 1736, 3024, 5024
PflFI GACNNNGTC 1 cut(s) 31
PflMI CCANNNNNTGG 2 cut(s) 1610, 3640
PinAI ACCGGT 2 cut(s) 63, 4241
Ppu21I YACGTR 2 cut(s) 202, 727
PsiI TTATAA 1 cut(s) 3129
Psp124BI GAGCTC 1 cut(s) 711
Psp1406I AACGTT 1 cut(s) 5238
Psp6I CCWGG 6 cut(s) 389, 510, 586, 1863, 4606, 5244
PspEI GGTNACC 1 cut(s) 4808
PspFI CCCAGC 2 cut(s) 1290, 2803
PspGI CCWGG 6 cut(s) 389, 510, 586, 1863, 4606, 5244
PspN4I GGNNCC 6 cut(s) 702, 1101, 1351, 3269, 3400, 3975
PspOMI GGGCCC 1 cut(s) 1099
PsrI GAACNNNNNNTAC 2 cut(s) 5139, 5171
PstI CTGCAG 3 cut(s) 1993, 2143, 2557
PstNI CAGNNNCTG 2 cut(s) 2552, 2884
PsuI RGATCY 7 cut(s) 669, 863, 1372, 1892, 3118, 3139, 3398
PsyI GACNNNGTC 1 cut(s) 31
PvuII CAGCTG 2 cut(s) 1505, 2033
RruI TCGCGA 1 cut(s) 2155
RsaI GTAC 5 cut(s) 856, 1422, 2734, 3269, 4445
RsaNI GTAC 5 cut(s) 855, 1421, 2733, 3268, 4444
Rsr2I CGGWCCG 1 cut(s) 3999
RsrII CGGWCCG 1 cut(s) 3999
SacI GAGCTC 1 cut(s) 711
SalI GTCGAC 1 cut(s) 5405
SapI GCTCTTC 1 cut(s) 4401
ScrFI CCNGG 7 cut(s) 391, 512, 588, 1865, 3403, 4608, 5246
SduI GDGCHC 4 cut(s) 711, 1103, 1354, 3074
SfcI CTRYAG 5 cut(s) 1797, 1989, 2139, 2553, 4227
SfuI TTCGAA 2 cut(s) 2597, 3544
SinI GGWCC 6 cut(s) 61, 331, 2947, 3364, 3814, 3999
SpeI ACTAGT 3 cut(s) 3259, 3325, 4051
SphI GCATGC 6 cut(s) 1225, 1862, 3790, 3845, 4190, 4740
SseBI AGGCCT 1 cut(s) 3051
SsiI CCGC 5 cut(s) 5, 165, 172, 719, 3443
SspI AATATT 1 cut(s) 2449
SstI GAGCTC 1 cut(s) 711
StuI AGGCCT 1 cut(s) 3051
StyD4I CCNGG 7 cut(s) 389, 510, 586, 1863, 3401, 4606, 5244
StyI CCWWGG 5 cut(s) 1872, 2484, 3519, 3817, 5066
TaqII GACCGA 1 cut(s) 3987
TatI WGTACW 2 cut(s) 2732, 4443
TseFI GTSAC 8 cut(s) 197, 452, 616, 1128, 2264, 2548, 3306, 3554
Tsp45I GTSAC 8 cut(s) 197, 452, 616, 1128, 2264, 2548, 3306, 3554
TspGWI ACGGA 2 cut(s) 727, 3503
Tth111I GACNNNGTC 1 cut(s) 31
Van91I CCANNNNNTGG 2 cut(s) 1610, 3640
Vha464I CTTAAG 1 cut(s) 3883
VpaK11BI GGWCC 6 cut(s) 61, 331, 2947, 3364, 3814, 3999
XbaI TCTAGA 1 cut(s) 887
XceI RCATGY 9 cut(s) 1225, 1829, 1862, 3790, 3845, 4190, 4740, 5036, 5162
XcmI CCANNNNNNNNNTGG 1 cut(s) 230
XmiI GTMKAC 2 cut(s) 1309, 5406
XmnI GAANNNNTTC 3 cut(s) 1736, 3024, 5024
Zsp2I ATGCAT 2 cut(s) 1276, 5225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.