Rroxscaffold_1G00027420

Proteasome-associated protein ECM29 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0006358
Physical Location & Seq
Reverse (-)
34625106 .. 34652164
27059 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00027420.1

Sequence Viewer

Length: 5601 bp
ATGTACGCCGGCGTCCCGCAAAGACGTGTGGAGGATGCCGTCCGGAGCTGCGCCTCCGCAAATCGGAGGGTGCTAGAAATACTGAGTCATGTGAACAAGCGAGTGAAGCATCAACCTGAGATTGGTTTGCCCTTGACTGAACTATGGACTATATACTCGGAAGCTCATTCTGCTCCAATGGTCAGGAATTTTTGCATTCTCTACCTCGAGATGGCAATGGATCGTGCTGATAGAAAGGAAAAAGAAAATTTGTCACCCATGCTTCTGTTAGGGGTTTCCAAGCTTCCTCAACAGCACCAAGAGATTATACTCAGACTTGTTATAAAGGTCATTGGTGAATGTCACACAAGCGGAATAGATGGAGAAGTTGCTGCAAAGTATGGACTGATAAACGATTCTCAAGACAGAAACGTTTTTATCGAATTTTGCCTTCATACAATCTTATATCAGCCGTCATCTCAAAGAGAATGCCCGCCAGGACTTTCAATTGCTCAAGCCAACCGCGTAACAGCTAGACAATCATTGAACAGTGATATGCTTCTAACAAGAAAGTTAGGGATCTTGAATGTTGTTGAGGCCATGGTGCTGGCCCCTGAGCTCGTTTATCCTTTGTACCTGACTGCCTCCATAGATTGTCAAGAGCTTGTCGTTAAGAGAGGAGAGGAGCTTGTAAAGAAGAAGGCTGCTGGAGCTAATTTAGATGATTCAGTCCTCATAAACAGACTTTTTTTGTTATTTAATGGAACTGCTACATCTCAAAATGTTGGTCCTGAATCTAGAGTCACTCCAGCAAGTCCTGCTTTGAAAGGAAAAATGATGTCTATTTTCTGCCGGTCTATTACAGCCGCTAACAGTTTCCCTTCAACCTTGCAGTGCATTTTTGGTTGCATATATGGAAGTGATACCACATCAAGGTTGAAGCAGTTGGGAATGGAATTCACTGTCTGGTCCATAATCGATCAACTTAAACTTATGGGCCCTGTCATTCTGAGTGGAATACTGAAGTCACTTGACACTGCTTCAAGTTCAGAATCAGATGCTACTCATAGGGACTCCAGAACTTTCGCTTATCAAGCTATTGGATTGCTTGCACAGCGGATGCCTCAGCTTTTCAGAGATACGACTGACATGGCTGTGCGTCTTTTTGATGCATTGAAGGTAGAGACCCAATATTTTCGCCTCAGTATTCAAGAAGCAACAAATTCTCTTGCCACTGCGTATAAGGAACAAAGTGAAGTAAGATTCTGCGCTATAAGATGGGCGACTTCTTTATTTGACTTACAACATTGTCCAAGCCGGTATATCTGTATGCTTGGGGCTGCTGATATTAAATTGGATATAAGGGAGATTGCATTTGAAGGCCTTTTTCCTATGAAAGATGATGGGTCAAGTATGAGTAAGATACAAGAGCTACATTACCCCAAACTTGGAGACATGTTAGATTATATTCTTTCACAGCAGCCAAACTTGTCAGAACCAGCTGAGATGCGGGAGCAGAAGCTTCAGTTTCCCTCAAAAACGTATCTAGTTATTATTGAATTTTTGTTGAAGTGCTTTGAGTCAGAATTGGAGCATAACAATTCAATTAAAGGATCAACTCAATTTCACTCGTCTGTAGAAGCAATGTGCTTACTTCTGGAACATGCTATGGCATATGAAGGGTCTGTTGAGTTGCATGCCAAGGCTTCCAATGCATTGATTGCAATTGGATCTCATATTCCAGAGCTTGTAGCTTCACGTTATGCAAAAAAAGTTCAATGGCTAAAACAATTATTGAGTCATATTGACTTAGATACCCGTGAAGCTGCAGCACGTTTACTTGGTATTGCTTCCTCCGTTCTTCTTCCAGCTGCTTCGTGTGATCTTATTTCTGAAATAATTGCCTCAGTAAGGGGAATACATAAGTTAAGGTTTGAGGTTCAACACGGAGCATTATGTGCATTAGGTTATGTAACTGCAGATTGTATCTCTAGAAAACCAGCTATCCCAGAGGCATTATTTCAAACTACCCTAAAGTTACTTGTCGATGTTGTTAATTCTGAGACTGCGACACTAGCATCAGTAGCGGTCCAAGCGTTAGGTCATATTGGACTGATCGTTGCATTGCCTTCACTTATTATTGAGTCTTGTTCAGTTGATATTCTGGTGCTTTTACAAGAAAGATTGAGAAAGCTACTTAAGGGTGATGATACCAAAGCAATTCAGAAAATTATTATATCCATTGGCCACATCTGTGTCAAGGAAACATCATCCGCGTGTCTTAATATTGCTCTGGAGTTAATATTCAGTCTTTCCCGATCTAAGGTTGAGGATATCCTCTTTGCTGCTGGCGAGGCACTGTCGTTTTTGTGGGGTGGTGTTCCGGTGACTGCTGATTTGATTCTCAGAACTAATTATTCACTCTCCATGGCTTCTAAGTTTCTCATGGGAGATGCGAGTTTATCTCTCTCAAAACATAGCCCCATGGAAATGAATGAAGCTAATAAAGATCGTGATGACATGGTTCGAGAAGCAATTACTAAAAAGCTTTTTGACGATCTTCTATACAGTACAAGAAAAGAAGATCGCTGTGCTGGAACTGTATGGCTATTATCAATTACCATGTATTGTGGCCATCAACCAGCTATTCAGAATATGCTGCCAGAAATTCAGGAGGCTTTTTCACACCTTCTTGGTGAACAGAATGAACTCACGCAGGAGCTGGCCTCACAAGGGATGAGCGTTGTGTATGAAATTGGTGATGCGTCAATGAAAGGAAACTTGGTAAATGCACTTGTAAACACTCTGACAGGTTCCGGGAAGAAAAAAAGAGCTATAAAGCTTGCTGAAGACTCTGAAGTTTTTCAAGAGGGAGTTATTGGTGAAGGTCTTAGTGGAGGCAAACTCAGTACATACAAGGAGCTGTGCAATGTAGCGAATGAGATGGGACAACCAGATCTGATTTATAAGTTTATGGATCTGGCTAATTATCAAACTTCTCTGAATTCAAAGAGGGGTGCTGCCTTTGGTTTTTCAAAGATAGCAAAGCAAGCAGGAGATGCTCTGAAGCCACACTTGCGATCCTTAATTCCAAGGCTTGTTCGTTATCAGTATGATCCTGACAAAAATGTGCAGGATGCTATGTCACACATATGGAAGTCACTGGTGGAAGATTCAAAGAAAACCATTGATGAACACTTGGACCTCATTATTGATGACTTGTTAATACAATGTGGATCTAGGCTTTGGCGCACACGAGAGGCATCCTGTCTTGCCCTTGCAGATATCATCCAGGGCCGGAAGTTTGATCAGGTTGGGAAGCATTTAAGGAAGTTATGGTCAGCAGCATTCCGTGCCATGGATGATATTAAAGAGACTGTTCGAAATTCTGGCGACAAGTTATGCCGTGTTTTAACTTCATTGACAGTGAGGCTTTCTGATGTCACCCTAACTGATGTATCAGATGCAAGCCAAACAATGGATCTTGTTCTGCCTCTTTTGCTTACAGAAGGCATACTAAGTAAAGTTGATAGTATTCGAAAGGCATCTATAGAGGTTGTTATGAAGCTTGCAAAGGGGGCTGGCATTGCAATTCGTCCACATTTATCTGATCTAGTTTGTTGCATGCTGGAAAGTTTATCGAGCCTTGAGGACCAAGGGCTTAATTACGTGGAGTTGCATGCAGCAAATGCTGGGATACAGACAGAGAAGCTCGAAAGTTTGAGAATTTCGATTGCAAAAGGCTCTCCCATGTGGGAAACTCTTGATCTCTGCATAAAAGTTGTGGATGCAGGATCATTAGATCAATTGGTTCCTCGTCTTGGTCAATTGGTTCGATCTGGGGTTGGTCTCAATACAAGACTGCAGAACACAAAAGGAGACAATGAGAGGACATATGGTAAGGAACCTGAGAGGTGTCTGATACTCCAGAGTGTGAGACGCGATAAGAACTGGGTTGGTGTTGCCAGCTTTATTACCTTACTAGTTCAAGAAGTTGGTGTTGAAATCAAACCATATACGAGCAAGCTACTGAGGCTGTTGTTTCCAGTTGTGAAGGACGAAAAAAGTGCTGCATCAAAACGAGCCTTTGCTGATGCATGTGCAGTACTTTTAAAACATACAGTAGCCTCTCAGGCCGAAAAGTTGATCGATGACACTGCAGCTTTGCATATTGGTGACCGAAATGCTCAAGTGGCGTGTGCGGTTTTATTGAAAAGCTATTCATCCAAGGCCTCAGACATTTTGGATGGATATCTTGCAGCTATACTTCCAGTAATTTTTATCTCAAGATTTGACGATGACAAGTATGTTTCTGGCCTATTTGAAGAGTTGTGGGAAGAGCATACAAGTAGTGAACGTGTAGCCCTCCAGTTATACTTGGCAGAAATTGTTTCTCTTATTTGTGAGAGCATTGCAACATCTTCTTGGGCAAGTAAAAAGAAGTCAGCCCAGGCCATTTCCAAACTCAGTGAAGTTTTGGGTGAATCTCTGGCTTCTTACTATAATGTCTTGCTCAAATCTCTTATGAAAGAGATTCCTGGTCGGTTATGGGAGGGAAAGGAAGCTCTATTGTATTCAATTGCTGCACTTTCTGTGTCTTGTCATAAAGCAATTTCAACTGATGATCCTCATACTCTGAATGAAGTTTTGAGGGTATTATCTTCAGCATGCACGAAAAAGGCAAAGAAATATCGTGAAGCAGCTCTATCCTGTCTCGAACAGGATGGTTTCACTTTGGCCTGGTTTGAGATTGTTGGATTTCCAAGTCTAGCATTGCTGGTTGGCTCCGGGTCAGATGCTGTAATGTTAACATGGTATCAAATCAAACTGTTCATTAGAACCACTCTGAGCCCGGGTTGGAATCATGATCGGGAGATTCATGCTGAGCCAGATGAATTCGATGAGGGAATTGGTCCTGTGTTCTCATTGTCACCCAATATCGATTCATGTGTGTGGCCCCACCTGGTTGTAAAAGCCTTTGGTAATCAAGAGTTCTTTAATGAGGCATTTCCATTGTTATATGATATGTGTAATGCTTCCACTCTCGGTGTTTCTGGGAAAGCAACTTTGGCTGGCAATGGTGCTAAAGCAGAAGAGGATCAAATTGAGCAGGCTCCTGTTTCACATGAGAAAATCTTGGACTGCATGACCGCTTGCATTCATGTGGCTAATATAAAAGATATTCTTGAACAACAGAAGAACTTGATGCATGTGTTGACTACTGCTCTGTCACTTGGATTTCCATGGACAGTCAAAATATCTGCCCTTTCATTGATAAAGGAACTTGTTTCAAGTGTCCAGAAGGTTCTGGGTGACTCCAAAGAAACTTATGCTCATGCCAACATAATTTTGTTAGTTCAGGAGTTGTTTCACTCAGTGGCACCTCAAGTTGTTGAATGCATAAGCACAATTAAAGTTGGGCAGGTTCATGTAACTGCTTCAGAGTGCCTTCTAGTGATCATCACGCTTTATGGAGACCTTCGGTCAGTTGATTCTACTAATGTACAATTTGAGGGTACGCTTCTGCATCTGTATGAAGTGGAGAAAAATGGCGAAGCAAAGTCCTCGTTGAAAAAATGTATGGATACCCTTGAAAACTTAAAACTAGAATGCCCGAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ecm29

Protein Analysis

1866

Amino Acids

206.0

Weight (kDa)

6.01

Isoelectric Point (pI)

41.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ECM29_N PF13001 19 - 410 2.4e-97 Proteasome component ECM29, N-terminal
ARM_ECM29 PF23702 564 - 661 6.5e-12 ECM29, ARM-like repeats
HEAT_GCN1 PF23271 1005 - 1129 1.1e-06 Stalled ribosome sensor GCN1-like, HEAT repeats
HEAT_ECM29 PF24492 1191 - 1281 5.3e-28 Proteasome adapter and scaffold protein ECM29, HEAT-repeat domain
HEAT_ECM29 PF24492 1312 - 1381 1e-09 Proteasome adapter and scaffold protein ECM29, HEAT-repeat domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 323, 2946
Acc36I ACCTGC 1 cut(s) 5392
AccB1I GGYRCC 1 cut(s) 5359
AccB7I CCANNNNNTGG 1 cut(s) 3457
AccII CGCG 3 cut(s) 504, 2255, 3921
AccIII TCCGGA 1 cut(s) 42
AclI AACGTT 1 cut(s) 411
AcoI YGGCCR 2 cut(s) 2224, 2611
AcuI CTGAAG 7 cut(s) 1022, 1487, 2847, 2856, 3065, 4626, 5403
AcyI GRCGYC 1 cut(s) 12
AdeI CACNNNGTG 1 cut(s) 5356
AfaI GTAC 7 cut(s) 5, 614, 2551, 2890, 4086, 5484, 5497
AflII CTTAAG 1 cut(s) 2177
AflIII ACRYGT 3 cut(s) 25, 1434, 4336
AhdI GACNNNNNGTC 1 cut(s) 5461
AhlI ACTAGT 1 cut(s) 3961
AjiI CACGTC 1 cut(s) 26
AjnI CCWGG 6 cut(s) 475, 3270, 4428, 4516, 4718, 4941
AjuI GAANNNNNNNTTGG 4 cut(s) 2744, 2776, 5030, 5062
AleI CACNNNNGTG 1 cut(s) 2232
Alw21I GWGCWC 1 cut(s) 600
Alw26I GTCTC 9 cut(s) 1157, 1425, 2036, 3347, 3833, 3852, 3910, 4697, 5448
AlwNI CAGNNNCTG 2 cut(s) 2701, 4778
Ama87I CYCGRG 3 cut(s) 206, 4831, 5593
Aor13HI TCCGGA 1 cut(s) 42
ApaI GGGCCC 1 cut(s) 980
ArsI GACNNNNNNTTYG 4 cut(s) 751, 783, 2359, 2391
Asp700I GAANNNNTTC 1 cut(s) 2841
AspLEI GCGC 3 cut(s) 53, 1250, 3231
AsuC2I CCSGG 4 cut(s) 2797, 4768, 4832, 4833
AsuII TTCGAA 2 cut(s) 3361, 3517
AvaI CYCGRG 3 cut(s) 206, 4831, 5593
AvaII GGWCC 6 cut(s) 767, 948, 2068, 3181, 3631, 4892
BaeGI GKGCMC 1 cut(s) 980
BaeI ACNNNNGTAYC 2 cut(s) 3420, 3453
BalI TGGCCA 2 cut(s) 2226, 2613
BanI GGYRCC 1 cut(s) 5359
BanII GRGCYC 3 cut(s) 600, 980, 4832
BauI CACGAG 1 cut(s) 3234
BbsI GAAGAC 1 cut(s) 2835
Bbv12I GWGCWC 1 cut(s) 600
BbvCI CCTCAGC 1 cut(s) 1104
BccI CCATC 8 cut(s) 205, 353, 1251, 1376, 2622, 2917, 4220, 4697
BceAI ACGGC 3 cut(s) 23, 436, 3369
BcgI CGANNNNNNTGC 6 cut(s) 4028, 4062, 4118, 4152, 5526, 5560
BciT130I CCWGG 6 cut(s) 477, 3272, 4430, 4518, 4720, 4943
BciVI GTATCC 2 cut(s) 3669, 5557
BclI TGATCA 2 cut(s) 3286, 5436
BcnI CCSGG 4 cut(s) 2797, 4768, 4832, 4833
BcoDI GTCTC 9 cut(s) 1157, 1425, 2036, 3347, 3833, 3852, 3910, 4697, 5448
BcuI ACTAGT 1 cut(s) 3961
BfmI CTRYAG 6 cut(s) 1614, 1806, 1956, 3528, 3842, 4137
BfrI CTTAAG 1 cut(s) 2177
BfuAI ACCTGC 1 cut(s) 5392
BfuI GTATCC 2 cut(s) 3669, 5557
BglI GCCNNNNNGGC 1 cut(s) 4112
BglII AGATCT 1 cut(s) 2935
BlpI GCTNAGC 1 cut(s) 4863
BmcAI AGTACT 1 cut(s) 4086
Bme18I GGWCC 6 cut(s) 767, 948, 2068, 3181, 3631, 4892
BmeRI GACNNNNNGTC 1 cut(s) 5461
BmeT110I CYCGRG 3 cut(s) 206, 4831, 5593
BmgBI CACGTC 1 cut(s) 26
BmiI GGNNCC 9 cut(s) 591, 978, 2794, 3792, 3885, 4765, 4937, 5094, 5361
BmrI ACTGGG 1 cut(s) 3940
BmuI ACTGGG 1 cut(s) 3940
BpiI GAAGAC 1 cut(s) 2835
BplI GAGNNNNNCTC 6 cut(s) 2428, 2460, 2690, 2722, 2868, 2900
BpmI CTGGAG 6 cut(s) 708, 771, 1039, 2294, 3890, 4331
Bpu10I CCTNAGC 2 cut(s) 594, 1104
Bpu1102I GCTNAGC 1 cut(s) 4863
Bpu14I TTCGAA 2 cut(s) 3361, 3517
BpuEI CTTGAG 6 cut(s) 384, 477, 3647, 4152, 4249, 5349
BpuMI CCSGG 4 cut(s) 2797, 4768, 4832, 4833
Bsa29I ATCGAT 3 cut(s) 957, 4128, 4920
BsaAI YACGTR 1 cut(s) 3649
BsaBI GATNNNNATC 1 cut(s) 4230
BsaHI GRCGYC 1 cut(s) 12
BsaI GGTCTC 3 cut(s) 1157, 3833, 5448
BsaWI WCCGGW 2 cut(s) 42, 2362
Bse118I RCCGGY 3 cut(s) 8, 831, 1296
Bse1I ACTGG 5 cut(s) 3147, 3935, 4025, 4250, 4348
Bse3DI GCAATG 8 cut(s) 222, 1629, 2102, 2914, 3564, 4389, 4751, 5062
Bse8I GATNNNNATC 1 cut(s) 4230
BseAI TCCGGA 1 cut(s) 42
BseBI CCWGG 6 cut(s) 477, 3272, 4430, 4518, 4720, 4943
BseCI ATCGAT 3 cut(s) 957, 4128, 4920
BseJI GATNNNNATC 1 cut(s) 4230
BseMI GCAATG 8 cut(s) 222, 1629, 2102, 2914, 3564, 4389, 4751, 5062
BseNI ACTGG 5 cut(s) 3147, 3935, 4025, 4250, 4348
BseRI GAGGAG 2 cut(s) 672, 677
BseSI GKGCMC 1 cut(s) 980
BseYI CCCAGC 1 cut(s) 3671
BsgI GTGCAG 3 cut(s) 3131, 4101, 4548
Bsh1236I CGCG 3 cut(s) 504, 2255, 3921
BshNI GGYRCC 1 cut(s) 5359
BshVI ATCGAT 3 cut(s) 957, 4128, 4920
BsiHKAI GWGCWC 1 cut(s) 600
BsiHKCI CYCGRG 3 cut(s) 206, 4831, 5593
BsiSI CCGG 9 cut(s) 9, 43, 832, 1297, 2363, 2796, 3277, 4767, 4832
BslFI GGGAC 2 cut(s) 1064, 2940
BsmAI GTCTC 9 cut(s) 1157, 1425, 2036, 3347, 3833, 3852, 3910, 4697, 5448
BsmBI CGTCTC 1 cut(s) 3910
BsmFI GGGAC 2 cut(s) 1064, 2940
BsmI GAATGC 6 cut(s) 195, 473, 3326, 5136, 5381, 5594
Bso31I GGTCTC 3 cut(s) 1157, 3833, 5448
BsoBI CYCGRG 3 cut(s) 206, 4831, 5593
Bsp119I TTCGAA 2 cut(s) 3361, 3517
Bsp120I GGGCCC 1 cut(s) 976
Bsp1286I GDGCHC 3 cut(s) 600, 980, 4832
Bsp13I TCCGGA 1 cut(s) 42
Bsp1407I TGTACA 1 cut(s) 5482
Bsp1720I GCTNAGC 1 cut(s) 4863
Bsp19I CCATGG 5 cut(s) 579, 2406, 2463, 3336, 5222
BspDI ATCGAT 3 cut(s) 957, 4128, 4920
BspEI TCCGGA 1 cut(s) 42
BspFNI CGCG 3 cut(s) 504, 2255, 3921
BspHI TCATGA 1 cut(s) 4843
BspLI GGNNCC 9 cut(s) 591, 978, 2794, 3792, 3885, 4765, 4937, 5094, 5361
BspMAI CTGCAG 4 cut(s) 1810, 1960, 3846, 4141
BspMI ACCTGC 1 cut(s) 5392
BspQI GCTCTTC 1 cut(s) 4311
BspT104I TTCGAA 2 cut(s) 3361, 3517
BspT107I GGYRCC 1 cut(s) 5359
BspTI CTTAAG 1 cut(s) 2177
BspTNI GGTCTC 3 cut(s) 1157, 3833, 5448
BsrDI GCAATG 8 cut(s) 222, 1629, 2102, 2914, 3564, 4389, 4751, 5062
BsrFI RCCGGY 3 cut(s) 8, 831, 1296
BsrGI TGTACA 1 cut(s) 5482
BsrI ACTGG 5 cut(s) 3147, 3935, 4025, 4250, 4348
BssAI RCCGGY 3 cut(s) 8, 831, 1296
BssNI GRCGYC 1 cut(s) 12
BssSI CACGAG 1 cut(s) 3234
BssT1I CCWWGG 9 cut(s) 579, 1680, 2406, 2463, 3071, 3336, 3634, 4206, 5222
Bst2BI CACGAG 1 cut(s) 3234
Bst2UI CCWGG 6 cut(s) 477, 3272, 4430, 4518, 4720, 4943
Bst6I CTCTTC 3 cut(s) 4299, 4311, 5067
BstACI GRCGYC 1 cut(s) 12
BstAFI CTTAAG 1 cut(s) 2177
BstAPI GCANNNNNTGC 6 cut(s) 797, 1700, 1937, 3038, 3332, 3668
BstAUI TGTACA 1 cut(s) 5482
BstBAI YACGTR 1 cut(s) 3649
BstBI TTCGAA 2 cut(s) 3361, 3517
BstDSI CCRYGG 5 cut(s) 579, 2406, 2463, 3336, 5222
BstEII GGTNACC 1 cut(s) 4154
BstENI CCTNNNNNAGG 1 cut(s) 1888
BstFNI CGCG 3 cut(s) 504, 2255, 3921
BstHHI GCGC 3 cut(s) 53, 1250, 3231
BstMAI GTCTC 9 cut(s) 1157, 1425, 2036, 3347, 3833, 3852, 3910, 4697, 5448
BstNI CCWGG 6 cut(s) 477, 3272, 4430, 4518, 4720, 4943
BstNSI RCATGY 8 cut(s) 1438, 1646, 1679, 3607, 3662, 4080, 4650, 5192
BstPI GGTNACC 1 cut(s) 4154
BstSFI CTRYAG 6 cut(s) 1614, 1806, 1956, 3528, 3842, 4137
BstSLI GKGCMC 1 cut(s) 980
BstUI CGCG 3 cut(s) 504, 2255, 3921
BstV2I GAAGAC 1 cut(s) 2835
BstX2I RGATCY 6 cut(s) 558, 1709, 2935, 2956, 3215, 3460
BstXI CCANNNNNNTGG 1 cut(s) 586
BstYI RGATCY 6 cut(s) 558, 1709, 2935, 2956, 3215, 3460
Bsu15I ATCGAT 3 cut(s) 957, 4128, 4920
BsuI GTATCC 2 cut(s) 3669, 5557
BsuTUI ATCGAT 3 cut(s) 957, 4128, 4920
BtgI CCRYGG 5 cut(s) 579, 2406, 2463, 3336, 5222
BtrI CACGTC 1 cut(s) 26
BtsI GCAGTG 4 cut(s) 878, 1014, 1212, 4134
BveI ACCTGC 1 cut(s) 5392
CaiI CAGNNNCTG 2 cut(s) 2701, 4778
CciI TCATGA 1 cut(s) 4843
CfoI GCGC 3 cut(s) 53, 1250, 3231
Cfr10I RCCGGY 3 cut(s) 8, 831, 1296
Cfr9I CCCGGG 1 cut(s) 4831
ClaI ATCGAT 3 cut(s) 957, 4128, 4920
CseI GACGC 3 cut(s) 1127, 2733, 3927
CsiI ACCWGGT 1 cut(s) 4941
Csp6I GTAC 7 cut(s) 4, 613, 2550, 2889, 4085, 5483, 5496
CviQI GTAC 7 cut(s) 4, 613, 2550, 2889, 4085, 5483, 5496
DraI TTTAAA 1 cut(s) 4092
DraIII CACNNNGTG 1 cut(s) 5356
DriI GACNNNNNGTC 1 cut(s) 5461
EaeI YGGCCR 2 cut(s) 2224, 2611
Eam1104I CTCTTC 3 cut(s) 4299, 4311, 5067
Eam1105I GACNNNNNGTC 1 cut(s) 5461
EarI CTCTTC 3 cut(s) 4299, 4311, 5067
Ecl136II GAGCTC 1 cut(s) 598
Eco130I CCWWGG 9 cut(s) 579, 1680, 2406, 2463, 3071, 3336, 3634, 4206, 5222
Eco147I AGGCCT 2 cut(s) 1362, 4211
Eco24I GRGCYC 3 cut(s) 600, 980, 4832
Eco31I GGTCTC 3 cut(s) 1157, 3833, 5448
Eco32I GATATC 3 cut(s) 2314, 3265, 4232
Eco47I GGWCC 6 cut(s) 767, 948, 2068, 3181, 3631, 4892
Eco53kI GAGCTC 1 cut(s) 598
Eco57I CTGAAG 7 cut(s) 1022, 1487, 2847, 2856, 3065, 4626, 5403
Eco88I CYCGRG 3 cut(s) 206, 4831, 5593
Eco91I GGTNACC 1 cut(s) 4154
EcoICRI GAGCTC 1 cut(s) 598
EcoNI CCTNNNNNAGG 1 cut(s) 1888
EcoO109I RGGNCCY 1 cut(s) 977
EcoO65I GGTNACC 1 cut(s) 4154
EcoRI GAATTC 3 cut(s) 935, 2983, 4874
EcoRII CCWGG 6 cut(s) 475, 3270, 4428, 4516, 4718, 4941
EcoRV GATATC 3 cut(s) 2314, 3265, 4232
EcoT14I CCWWGG 9 cut(s) 579, 1680, 2406, 2463, 3071, 3336, 3634, 4206, 5222
EcoT22I ATGCAT 5 cut(s) 1153, 1696, 4078, 5190, 5381
EcoT38I GRGCYC 3 cut(s) 600, 980, 4832
ErhI CCWWGG 9 cut(s) 579, 1680, 2406, 2463, 3071, 3336, 3634, 4206, 5222
Esp3I CGTCTC 1 cut(s) 3910
FalI AAGNNNNNCTT 6 cut(s) 784, 816, 3038, 3070, 5148, 5180
FaqI GGGAC 2 cut(s) 1064, 2940
FauI CCCGC 3 cut(s) 24, 480, 1482
FauNDI CATATG 3 cut(s) 1654, 3131, 3874
FbaI TGATCA 2 cut(s) 3286, 5436
FriOI GRGCYC 3 cut(s) 600, 980, 4832
GlaI GCGC 3 cut(s) 52, 1249, 3230
GsaI CCCAGC 1 cut(s) 3675
GsuI CTGGAG 6 cut(s) 708, 771, 1039, 2294, 3890, 4331
HapII CCGG 9 cut(s) 9, 43, 832, 1297, 2363, 2796, 3277, 4767, 4832
HgaI GACGC 3 cut(s) 1127, 2733, 3927
HhaI GCGC 3 cut(s) 53, 1250, 3231
Hin1I GRCGYC 1 cut(s) 12
Hin6I GCGC 3 cut(s) 51, 1248, 3229
HinP1I GCGC 3 cut(s) 51, 1248, 3229
HincII GTYRAC 2 cut(s) 4788, 5196
HindII GTYRAC 2 cut(s) 4788, 5196
HindIII AAGCTT 5 cut(s) 281, 1499, 2525, 2819, 3545
HpaI GTTAAC 1 cut(s) 4788
HpaII CCGG 9 cut(s) 9, 43, 832, 1297, 2363, 2796, 3277, 4767, 4832
Hpy166II GTNNAC 8 cut(s) 94, 1817, 2678, 2779, 3578, 4334, 4788, 5196
Hpy8I GTNNAC 8 cut(s) 94, 1817, 2678, 2779, 3578, 4334, 4788, 5196
HpyCH4IV ACGT 7 cut(s) 25, 411, 1520, 1738, 1813, 3648, 4336
HpySE526I ACGT 7 cut(s) 25, 411, 1520, 1738, 1813, 3648, 4336
Hsp92I GRCGYC 1 cut(s) 12
HspAI GCGC 3 cut(s) 51, 1248, 3229
Kpn2I TCCGGA 1 cut(s) 42
KroI GCCGGC 1 cut(s) 8
KroNI GCCGGC 1 cut(s) 10
Ksp22I TGATCA 2 cut(s) 3286, 5436
KspAI GTTAAC 1 cut(s) 4788
LguI GCTCTTC 1 cut(s) 4311
MabI ACCWGGT 1 cut(s) 4941
MaeII ACGT 7 cut(s) 25, 411, 1520, 1738, 1813, 3648, 4336
MfeI CAATTG 5 cut(s) 486, 1704, 3785, 3806, 4557
MflI RGATCY 6 cut(s) 558, 1709, 2935, 2956, 3215, 3460
MhlI GDGCHC 3 cut(s) 600, 980, 4832
MlsI TGGCCA 2 cut(s) 2226, 2613
MluNI TGGCCA 2 cut(s) 2226, 2613
MlyI GAGTC 8 cut(s) 94, 789, 1046, 1568, 1786, 2132, 2825, 5288
MmeI TCCRAC 2 cut(s) 4714, 4817
Mox20I TGGCCA 2 cut(s) 2226, 2613
Mph1103I ATGCAT 5 cut(s) 1153, 1696, 4078, 5190, 5381
MreI CGCCGGCG 1 cut(s) 8
MroI TCCGGA 1 cut(s) 42
MroNI GCCGGC 1 cut(s) 8
MroXI GAANNNNTTC 1 cut(s) 2841
MscI TGGCCA 2 cut(s) 2226, 2613
MslI CAYNNNNRTG 9 cut(s) 1133, 2232, 2254, 2468, 3130, 4152, 4930, 5141, 5511
Msp20I TGGCCA 2 cut(s) 2226, 2613
MspA1I CMGCKG 3 cut(s) 1096, 1481, 1850
MspCI CTTAAG 1 cut(s) 2177
MspI CCGG 9 cut(s) 9, 43, 832, 1297, 2363, 2796, 3277, 4767, 4832
MunI CAATTG 5 cut(s) 486, 1704, 3785, 3806, 4557
Mva1269I GAATGC 6 cut(s) 195, 473, 3326, 5136, 5381, 5594
MvaI CCWGG 6 cut(s) 477, 3272, 4430, 4518, 4720, 4943
MvnI CGCG 3 cut(s) 504, 2255, 3921
NaeI GCCGGC 1 cut(s) 10
NciI CCSGG 4 cut(s) 2797, 4768, 4832, 4833
NcoI CCATGG 5 cut(s) 579, 2406, 2463, 3336, 5222
NdeI CATATG 3 cut(s) 1654, 3131, 3874
NgoMIV GCCGGC 1 cut(s) 8
NlaIV GGNNCC 9 cut(s) 591, 978, 2794, 3792, 3885, 4765, 4937, 5094, 5361
NsiI ATGCAT 5 cut(s) 1153, 1696, 4078, 5190, 5381
NspI RCATGY 8 cut(s) 1438, 1646, 1679, 3607, 3662, 4080, 4650, 5192
NspV TTCGAA 2 cut(s) 3361, 3517
OliI CACNNNNGTG 1 cut(s) 2232
PaeI GCATGC 4 cut(s) 1679, 3607, 3662, 4650
PaeR7I CTCGAG 1 cut(s) 206
PagI TCATGA 1 cut(s) 4843
PceI AGGCCT 2 cut(s) 1362, 4211
PciI ACATGT 1 cut(s) 1434
PciSI GCTCTTC 1 cut(s) 4311
PcsI WCGNNNNNNNCGW 1 cut(s) 417
PctI GAATGC 6 cut(s) 195, 473, 3326, 5136, 5381, 5594
PdiI GCCGGC 1 cut(s) 10
PdmI GAANNNNTTC 1 cut(s) 2841
PflMI CCANNNNNTGG 1 cut(s) 3457
PfoI TCCNGGA 1 cut(s) 2795
PleI GAGTC 8 cut(s) 93, 788, 1046, 1567, 1785, 2131, 2825, 5288
PpsI GAGTC 8 cut(s) 93, 788, 1046, 1567, 1785, 2131, 2825, 5288
Ppu21I YACGTR 1 cut(s) 3649
PscI ACATGT 1 cut(s) 1434
PsiI TTATAA 2 cut(s) 323, 2946
Psp124BI GAGCTC 1 cut(s) 600
Psp1406I AACGTT 1 cut(s) 411
Psp6I CCWGG 6 cut(s) 475, 3270, 4428, 4516, 4718, 4941
PspEI GGTNACC 1 cut(s) 4154
PspFI CCCAGC 1 cut(s) 3671
PspGI CCWGG 6 cut(s) 475, 3270, 4428, 4516, 4718, 4941
PspN4I GGNNCC 9 cut(s) 591, 978, 2794, 3792, 3885, 4765, 4937, 5094, 5361
PspOMI GGGCCC 1 cut(s) 976
PstI CTGCAG 4 cut(s) 1810, 1960, 3846, 4141
PstNI CAGNNNCTG 2 cut(s) 2701, 4778
PsuI RGATCY 6 cut(s) 558, 1709, 2935, 2956, 3215, 3460
PvuII CAGCTG 2 cut(s) 1481, 1850
RsaI GTAC 7 cut(s) 5, 614, 2551, 2890, 4086, 5484, 5497
RsaNI GTAC 7 cut(s) 4, 613, 2550, 2889, 4085, 5483, 5496
RseI CAYNNNNRTG 9 cut(s) 1133, 2232, 2254, 2468, 3130, 4152, 4930, 5141, 5511
SacI GAGCTC 1 cut(s) 600
SapI GCTCTTC 1 cut(s) 4311
ScaI AGTACT 1 cut(s) 4086
SchI GAGTC 8 cut(s) 94, 789, 1046, 1568, 1786, 2132, 2825, 5288
SduI GDGCHC 3 cut(s) 600, 980, 4832
SexAI ACCWGGT 1 cut(s) 4941
SfcI CTRYAG 6 cut(s) 1614, 1806, 1956, 3528, 3842, 4137
Sfr274I CTCGAG 1 cut(s) 206
SfuI TTCGAA 2 cut(s) 3361, 3517
SgrAI CRCCGGYG 1 cut(s) 8
SinI GGWCC 6 cut(s) 767, 948, 2068, 3181, 3631, 4892
SlaI CTCGAG 1 cut(s) 206
SmaI CCCGGG 1 cut(s) 4833
SmiMI CAYNNNNRTG 9 cut(s) 1133, 2232, 2254, 2468, 3130, 4152, 4930, 5141, 5511
SmlI CTYRAG 8 cut(s) 206, 399, 492, 2177, 3626, 4167, 4264, 5364
SmoI CTYRAG 8 cut(s) 206, 399, 492, 2177, 3626, 4167, 4264, 5364
SpeI ACTAGT 1 cut(s) 3961
SphI GCATGC 4 cut(s) 1679, 3607, 3662, 4650
SseBI AGGCCT 2 cut(s) 1362, 4211
SspI AATATT 3 cut(s) 1172, 2266, 2283
SstI GAGCTC 1 cut(s) 600
StuI AGGCCT 2 cut(s) 1362, 4211
StyI CCWWGG 9 cut(s) 579, 1680, 2406, 2463, 3071, 3336, 3634, 4206, 5222
TaiI ACGT 7 cut(s) 28, 414, 1523, 1741, 1816, 3651, 4339
TaqII GACCGA 2 cut(s) 4173, 5451
TatI WGTACW 4 cut(s) 2549, 2888, 4084, 5482
TauI GCSGC 1 cut(s) 848
TspGWI ACGGA 3 cut(s) 1825, 1941, 3320
TspMI CCCGGG 1 cut(s) 4831
Van91I CCANNNNNTGG 1 cut(s) 3457
Vha464I CTTAAG 1 cut(s) 2177
VpaK11BI GGWCC 6 cut(s) 767, 948, 2068, 3181, 3631, 4892
XagI CCTNNNNNAGG 1 cut(s) 1888
XbaI TCTAGA 2 cut(s) 776, 1970
XceI RCATGY 8 cut(s) 1438, 1646, 1679, 3607, 3662, 4080, 4650, 5192
XhoI CTCGAG 1 cut(s) 206
XmaI CCCGGG 1 cut(s) 4831
XmnI GAANNNNTTC 1 cut(s) 2841
ZrmI AGTACT 1 cut(s) 4086
Zsp2I ATGCAT 5 cut(s) 1153, 1696, 4078, 5190, 5381
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.