RchiOBHm_Chr5g0051931

Proteasome-associated protein ECM29 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0006358
Physical Location & Seq
Reverse (-)
53394606 .. 53419163
24558 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ32937

Sequence Viewer

Length: 5448 bp
ATGGCGGAAACATCGGCTGTATCATCTTCCAAATCAGATGAAGAGAAAGAAGAGATGCTCGACCGGTTGCTCACCCGCCTCGCTCTCTGCGACGACTCCAACCTCCAACCTCTACTCTCCAAGCTCCTCCCCTATACTATTTCCTCCCTTTCTTCCAACTCCTCCGCCGTCCGGAACAAGGTGCTAGAAATACTTAGTCATGTGAACAAGCGAGTGAAGCATCAACCTGAGATTGGTTTGCCCTTGACTGAATTATGGACTATATACTCGGAAGCTCATTCTGCTCCAATGGTCAGGAATTTCTGCATTCTCTACCTCGAGATGGCAATGGATCGTGCCGATAGAAAGGAAAAAGAAAATTTGTCACCCATGCTTCTGTTAGGGGTTTCCAAGCTTCCTCAACAGCACCAAGAAATTATACTCAGACTTGTTGTAAAGGTCATTGGTGAATGTCACACAAGCGGAATAGATGGAGAAGTTGCTGCAAAGTATGGACTGGTAAACGATTCTCAAGACAGAAACGTTTTTATCGAATTTTGCCTTCACACAATCTTATATCAGCCGTCATCTCAAAGAGAATGCCCGCCAGGACTTTCAATTGCTCAAGCCAACCGCGTAACAGCTAGACAATCATTGAACAGTGATATGCTTCTAACAAGAAAGTTAGGGATCTTGAATGTTGTTGAGGCCATGGAGCTGGCCCCTGAGCTCGTTTATCCTTTGTACCTGACTGCCTCCATAGATTGTCAAGAGCTTGTCGTCAAGAGAGGAGAGGAGCTTGTAAAGAAGAAGGCTGCTGGAGCTAATTTAGATGATTCAGTCCTCATAAACAGACTTTTTTTGTTATTTAATGGAACTGCTACATCTCAAAATGTTGGTCCTGAATCTAGAGTCACTCCAGCAAGTCCTGCTTTGAAAGGAAAAATGATGTCTATTTTCTGCCGGTCTATTACAGCCGCTAACAGTTTCCCTTCAACCTTGCAGTGCATTTTTGGTTGCATATATGGAAGTGATACCACATCAAGGTTGAAGCAGTTGGGAATGGAATTCACTGTCTGGGTATTTAAACATTCCATAATCGATCAACTTAAACTTATGGGCCCTGTCATTCTGAGTGGAATACTGAAGTCACTTGACACTGCTTCAAGTTCAGAATCAGATGCTACTCATAGGGACTCCAGAACTTTCGCTTATCAAGCTATTGGATTGCTTGCACAGCGGATGCCTCAGCTTTTCAGAGATACGACTGACATGGCTGTGCGTCTTTTTGATGCATTGAAGGTAGAGACCCAATATTTTCGCCTCAGTATTCAAGAAGCAACAAATTCTCTTTCCACTGCGTATAAGGGAGCCCCCTCAACAGTACTAAAGGATCTGGAGACTCTTTTGCTCAAGTATACTCAAGAGGAACAAAGTGAAGTAAGATTCTGCGCTATAAGATGGGCGACTTCTTTATTTGACTTACAACATTGTCCAAGCCGGTATATCTGTATGCTTGGGGCTGCTGATATTAAATTGGATATAAGGGAGATTGCATTTGAAGGCCTTTTTCCTATGAAAGATGATGGGTCAAGTATGAGTAAGATACAAGAGCTACATTACCCCAAACTTGGAGACATGTTAGATTATATTCTTTCACAGCAGCCAAACTTGTCAGAACCAGCTGAGATACGGGAGCAGAAGCTTCAGTTTCCCTCAAAAACGTATCTAGTTATTATTGAATTTTTGTTGAAGTGCTTTGAGTCAGAATTGGAGCATAACAATTCAATTAAAGGATCAACTCAGTTTCACTCGTCTGTAGAAGCAATGTGCTTACTTCTGGAACATGCTATGGCATATGAAGGGTCTGTTGAGTTGCATGCCAAGGCTTCCAATGCATTGATTGCAATTGGATCTCATATTCCAGAGCTTGTAGCTTCACGTTATGCAAAAAAAGTTCCATGGCTAAAACAATTATTGAGTCATATTGACTTAGATACCCGTGAAGCTGCAGCACGTTTACTTGGTATTGCTTCCTCTGTTCTTCCTCCAGCTGCTTCATGTGATCTTATTTCTGAAATAATTGCCTCAGTAAGGGGAATACATAAGTTAAGGTTTGAGGTTCAACACGGAGCATTATGTGCATTAGGTTATGTAACTGCAGATTGTATGTCTAGAAAACCAGCTATCCCAGAGGCATTATTTCAAACTACCCTAAAGTTACTTGTCGATGTTGTTAATTCTGAGACTGCGACACTAGCATCAGTAGCGGTCCAAGCGTTAGGTCATATTGGACTGATCGTTGCATTGCCTTCACTTATTATTGAGTCTTGTTCAGTTGATATTCTGGTGCTTTTACAAGAAAGATTGAGAAAGCTACTTAAGGGTGATGATACCAAAGCAATTCAGAAAATTATTATATCCATTGGCCACATCTGTGTCAAGGAAACATCATCCGCGTGTCTTAATATTGCTCTGGAGTTAATATTCAGTCTTTCCCGATCTAAGGTTGAGGATATCCTCTTTGCCGCTGGCGAGGCACTGTCGTTTTTGTGGGGTGGTGTTCCAGTGACTGCTGATTTGATTCTCAGAACTAATTATTCACTCTCCATGGCTTCTAAGTTTCTCATGGGAGAAGCGAGTTTATCTCTCTCAAAACATAGCCCCATGGAAATGAATGAAGCTAATAAAGATCGTGATGACATGGTTCGAGAAGCAATTACTAAAAAGCTTTTTGACGATCTTCTATACAGTACAAGAAAAGAAGATCGCTGTGCTGGAACTGTATGGCTATTATCAATTACCATGTATTGTGGCCATCAACCAGCTATTCAGAAAATGCTGCCAGAAATTCAGGAGGCTTTTTCACACCTTCTTGGAGAACAGAATGAACTCACGCAGGAGCTGGCCTCACAAGGGATGAGCGTTGTGTATGAAATTGGTGATGCATCAATGAAAGGAAACTTGGTAAATGCACTTGTAAACACTCTGACAGGTTCCGGGAAGAAAAAAAGGGCTATAAAGCTTGCTGAAGACTCTGAAGTTTTTCAAGAGGGAGTTATTGGTGAAGGTCTTAGTGGAGGCAAACTCAGTACATACAAGGAGCTGTGCAATGTAGCGAATGAGATGGGACAACCAGATCTGATTTATAAGTTTATGGATCTGGCTAATTATCAAACTTCTCTGAATTCAAAAAGGGGTGCTGCCTTTGGTTTTTCAAAGATAGCAAAGCAAGCAGGTGATGCTCTGAAGCCACACTTGCGGTCCTTAATTCCAAGGCTTGTTCGTTATCAGTACGATCCTGACAAAAATGTGCAGGATGCTATGTCACACATATGGAAGTCACTGGTGGAAGATTCAAAGAAAACCATTGATGAACACTTGGACCTCATTATTGATGACTTGTTAATACAATGTGGATCTAGGCTTTGGCGCACACGAGAGGCATCCTGTCTTGCCCTTGCAGATATCATCCAGGGCCGGAAGTTTGATCAGGTTGGGAAGCATTTAAGGAAGTTATGGTCAGCAGCATTCCGTGCCATGGATGATATTAAAGAGACTGTTCGAAATTCTGGCGACAAGTTATGCCGTGTTTTAACTTCATTGACGGTGAGGCTTTCTGATGTCACCCTAACTGATGTATCAGATGCAAGCCAAACAATGGATCTTGTTCTGCCTCTTTTGCTTACAGAAGGCATACTAAGTAAAGTTGATAGTATTCGAAAGGCATCTATCGAGGTTGTTATGAAGCTTGCAAAGGGGGCTGGCATTGCAATTCGTCCACATTTATCTGATCTAGTTTGTTGCATGCTGGAAAGTTTATCGAGCCTTGAGGACCAAGGGCTTAATTACGTGGAGTTGCATGCAGCAAATGCTGGGATACAGACAGAGAAGCTCGAAAGTTTGAGAATTTCGATCGCAAAAGGCTCTCCCATGTGGGAAACTCTTGATCTCTGCATAAAAGTCGTGGATGCAGGATCATTAGATCAATTGGTTCCTCGTCTTGGTCAATTGGTTCGATCTGGGGTTGGTCTCAATACAAGGGTTGGTGTTGCCAGCTTTATTACCTTACTAGTTCAAGAAGTTGGTGTTGAAATCAAACCATATACGAGCAAGCTACTGAGGCTGTTGTTTCCAGTTGTGAAGGACGAAAAAAGTGCTGCATCAAAACGAGCCTTTGCTGATGCATGTGCAGTACTTTTAAAACATACAGTAGCCTCTCAGGCCGAAAAGTTGATCGATGACACTGCAGCTTTGCATATTGGTGACCGAAATGCTCAAGTGGCGTGTGCAGTTTTATTGAAAAGCTATTCATCCAAGGCCTCAGACATTTTGGATAGATATCTTGCAGCTATACTTCCAGTAATTTTTATCTCAAGATTTGACGATGACAAGTATGTTTCTGGCCTGTTTGAAGAGTTGTGGGAAGAGCATACAAGTAGTGAACGTGTAGCCCTCCAGTTATACTTGGCAGAAATTGTTTCTCTTATTTGTGGGAGCATTGCAACATCTTCTTGGGCAAGTAAAAAGAAGTCAGCCCAGGCCATTTCCAAAATCAGTGAAGTTTTGGGTGAATCTCTGGCTTCTTACTATAATGTCTTGCTCAAATCTCTTATGAAAGAGATTCCTGGTCGGTTATGGGAGGGAAAGGAAGCTCTATTGTATTCAATTGCTGCACTTTCTGTGTCTTGTCATAAAGCAATTTCAACTGATGATCCTCATACTCTGAATGAAGTTTTGAGGGTATTATCTTCAGCATGCACGAAAAAGGCAAAGAAATATCGTGAAGCAGCTCTATCCTGTCTTGAACAGGTTGTAAAAGCCTTTGGTAATCAAGAGTTCTTTAATGAGGCATTTCCATTGTTATACGATATGTGTAATGCTTCCACTCTCAGTGTTTCTGGGAAAGCAACTTTGGCTGGCAATGGTGCTAAAGCAGAAGAGGATCAAATTGAGCAGGTTCCTGTTTCACATGAGAAAATCTTGGACTGCATGACCGCTTGCATTCATGTGGCTAATATAAAAGATATTCTTGAACAACATAAGAACTTGATGCATGTGTTGACTACTGCTCTGTCACCTGGATTTCCATGGACAGTCAAAATATCTGCCCTTTCATTGATAAAGGAACTTGTTTCAAGTTTCCAGAAGGTTCTGGGTGACTCCAAAGAAACTTATGCTCATGCCAACATAATTTTGTTAGTTCGGGAGTTGTTTCACTCAGTGGCACCTCAAGTTGTCGAATGCATAAGCACAATTAAAGTTGGGCAGGTTCATGTAACTGCTTCAGAGTGCCTTCTAGTGATCATCACGCTTTATGGAGACCTTCGGTCAATTGACTCTACTAATGTACAATTTGAGGGTACGCTTCTGCATCTGTACGAAGTGGAGAAAAATGGCGAAGCAAAGTCCTTGTTGAAAAAATGTATGGATACCCTTGAAAACTTAAAACTAGAATGCCCGAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ecm29

Protein Analysis

1815

Amino Acids

200.11

Weight (kDa)

6.11

Isoelectric Point (pI)

41.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ECM29_N PF13001 20 - 500 1.5e-132 Proteasome component ECM29, N-terminal
ARM_ECM29 PF23702 625 - 754 2e-12 ECM29, ARM-like repeats
HEAT_GCN1 PF23271 1066 - 1191 9.7e-07 Stalled ribosome sensor GCN1-like, HEAT repeats
HEAT_ECM29 PF24492 1252 - 1411 3.7e-47 Proteasome adapter and scaffold protein ECM29, HEAT-repeat domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3129
AarI CACCTGC 1 cut(s) 3206
Acc36I ACCTGC 3 cut(s) 3206, 4927, 5239
AccB1I GGYRCC 1 cut(s) 5206
AccB7I CCANNNNNTGG 1 cut(s) 3640
AccI GTMKAC 1 cut(s) 1397
AccII CGCG 2 cut(s) 615, 2438
AccIII TCCGGA 1 cut(s) 171
AclI AACGTT 1 cut(s) 522
AcoI YGGCCR 2 cut(s) 2407, 2794
AcuI CTGAAG 7 cut(s) 1145, 1670, 3030, 3039, 3248, 4716, 5250
AdeI CACNNNGTG 1 cut(s) 5203
AfaI GTAC 9 cut(s) 725, 1365, 2734, 3073, 3275, 4176, 5331, 5344, 5360
AflII CTTAAG 1 cut(s) 2360
AflIII ACRYGT 2 cut(s) 1617, 4426
AgeI ACCGGT 1 cut(s) 63
AhdI GACNNNNNGTC 1 cut(s) 5308
AhlI ACTAGT 1 cut(s) 4051
AjnI CCWGG 5 cut(s) 586, 3453, 4518, 4606, 5059
AjuI GAANNNNNNNTTGG 4 cut(s) 2927, 2959, 4877, 4909
AleI CACNNNNGTG 1 cut(s) 2415
Alw21I GWGCWC 1 cut(s) 711
Alw26I GTCTC 7 cut(s) 1280, 1373, 1608, 2219, 3530, 4016, 5295
AlwNI CAGNNNCTG 2 cut(s) 2552, 2884
Ama87I CYCGRG 2 cut(s) 317, 5440
Aor13HI TCCGGA 1 cut(s) 171
ApaI GGGCCC 1 cut(s) 1103
ArsI GACNNNNNNTTYG 4 cut(s) 862, 894, 2542, 2574
AsiGI ACCGGT 1 cut(s) 63
Asp700I GAANNNNTTC 1 cut(s) 3024
AspLEI GCGC 2 cut(s) 1433, 3414
AspS9I GGNCC 9 cut(s) 700, 878, 1099, 1100, 2251, 3243, 3364, 3457, 3814
AsuC2I CCSGG 1 cut(s) 2980
AsuII TTCGAA 2 cut(s) 3544, 3700
AvaI CYCGRG 2 cut(s) 317, 5440
AvaII GGWCC 5 cut(s) 878, 2251, 3243, 3364, 3814
BaeGI GKGCMC 1 cut(s) 1103
BaeI ACNNNNGTAYC 2 cut(s) 3603, 3636
BalI TGGCCA 2 cut(s) 2409, 2796
BanI GGYRCC 1 cut(s) 5206
BanII GRGCYC 3 cut(s) 711, 1103, 1354
BauI CACGAG 1 cut(s) 3417
BbsI GAAGAC 1 cut(s) 3018
Bbv12I GWGCWC 1 cut(s) 711
BbvCI CCTCAGC 1 cut(s) 1227
BccI CCATC 6 cut(s) 316, 464, 1434, 1559, 2805, 3100
BceAI ACGGC 3 cut(s) 152, 547, 3552
BcgI CGANNNNNNTGC 6 cut(s) 3925, 3959, 4118, 4152, 4208, 4242
BciT130I CCWGG 5 cut(s) 588, 3455, 4520, 4608, 5061
BciVI GTATCC 2 cut(s) 3852, 5404
BclI TGATCA 2 cut(s) 3469, 5283
BcnI CCSGG 1 cut(s) 2980
BcoDI GTCTC 7 cut(s) 1280, 1373, 1608, 2219, 3530, 4016, 5295
BcuI ACTAGT 1 cut(s) 4051
BfmI CTRYAG 4 cut(s) 1797, 1989, 2139, 4227
BfrI CTTAAG 1 cut(s) 2360
BfuAI ACCTGC 3 cut(s) 3206, 4927, 5239
BfuI GTATCC 2 cut(s) 3852, 5404
BglI GCCNNNNNGGC 1 cut(s) 4202
BglII AGATCT 1 cut(s) 3118
BmcAI AGTACT 2 cut(s) 1365, 4176
Bme1390I CCNGG 6 cut(s) 588, 2980, 3455, 4520, 4608, 5061
Bme18I GGWCC 5 cut(s) 878, 2251, 3243, 3364, 3814
BmeRI GACNNNNNGTC 1 cut(s) 5308
BmeT110I CYCGRG 2 cut(s) 317, 5440
BmgT120I GGNCC 9 cut(s) 700, 878, 1099, 1100, 2251, 3243, 3364, 3457, 3814
BmiI GGNNCC 7 cut(s) 702, 1101, 1351, 2977, 3975, 4941, 5208
BmrFI CCNGG 6 cut(s) 588, 2980, 3455, 4520, 4608, 5061
BpiI GAAGAC 1 cut(s) 3018
BplI GAGNNNNNCTC 6 cut(s) 2611, 2643, 2873, 2905, 3051, 3083
BpmI CTGGAG 7 cut(s) 819, 882, 1162, 1397, 2013, 2477, 4421
Bpu10I CCTNAGC 2 cut(s) 705, 1227
Bpu14I TTCGAA 2 cut(s) 3544, 3700
BpuEI CTTGAG 8 cut(s) 495, 588, 1376, 1386, 3830, 4242, 4339, 5196
BpuMI CCSGG 1 cut(s) 2980
Bsa29I ATCGAT 2 cut(s) 1080, 4218
BsaAI YACGTR 1 cut(s) 3832
BsaBI GATNNNNATC 1 cut(s) 4320
BsaI GGTCTC 3 cut(s) 1280, 4016, 5295
BsaWI WCCGGW 2 cut(s) 63, 171
Bse118I RCCGGY 3 cut(s) 63, 942, 1479
Bse1I ACTGG 6 cut(s) 501, 2546, 3330, 4115, 4340, 4438
Bse3DI GCAATG 7 cut(s) 333, 1812, 2285, 3097, 3747, 4479, 4909
Bse8I GATNNNNATC 1 cut(s) 4320
BseAI TCCGGA 1 cut(s) 171
BseBI CCWGG 5 cut(s) 588, 3455, 4520, 4608, 5061
BseCI ATCGAT 2 cut(s) 1080, 4218
BseGI GGATG 9 cut(s) 1227, 2432, 2904, 3304, 3425, 3450, 3529, 3955, 4292
BseJI GATNNNNATC 1 cut(s) 4320
BseMI GCAATG 7 cut(s) 333, 1812, 2285, 3097, 3747, 4479, 4909
BseNI ACTGG 6 cut(s) 501, 2546, 3330, 4115, 4340, 4438
BseRI GAGGAG 4 cut(s) 116, 151, 783, 788
BseSI GKGCMC 1 cut(s) 1103
BseYI CCCAGC 1 cut(s) 3854
BsgI GTGCAG 4 cut(s) 3314, 4191, 4290, 4638
Bsh1236I CGCG 2 cut(s) 615, 2438
Bsh1285I CGRYCG 2 cut(s) 64, 3897
BshNI GGYRCC 1 cut(s) 5206
BshTI ACCGGT 1 cut(s) 63
BshVI ATCGAT 2 cut(s) 1080, 4218
BsiEI CGRYCG 2 cut(s) 64, 3897
BsiHKAI GWGCWC 1 cut(s) 711
BsiHKCI CYCGRG 2 cut(s) 317, 5440
BsiSI CCGG 6 cut(s) 64, 172, 943, 1480, 2979, 3460
BslFI GGGAC 2 cut(s) 1187, 3123
BsmAI GTCTC 7 cut(s) 1280, 1373, 1608, 2219, 3530, 4016, 5295
BsmFI GGGAC 2 cut(s) 1187, 3123
BsmI GAATGC 6 cut(s) 306, 584, 3509, 4983, 5228, 5441
Bso31I GGTCTC 3 cut(s) 1280, 4016, 5295
BsoBI CYCGRG 2 cut(s) 317, 5440
Bsp119I TTCGAA 2 cut(s) 3544, 3700
Bsp120I GGGCCC 1 cut(s) 1099
Bsp1286I GDGCHC 3 cut(s) 711, 1103, 1354
Bsp13I TCCGGA 1 cut(s) 171
Bsp1407I TGTACA 1 cut(s) 5329
Bsp19I CCATGG 6 cut(s) 690, 1940, 2589, 2646, 3519, 5069
BspDI ATCGAT 2 cut(s) 1080, 4218
BspEI TCCGGA 1 cut(s) 171
BspFNI CGCG 2 cut(s) 615, 2438
BspLI GGNNCC 7 cut(s) 702, 1101, 1351, 2977, 3975, 4941, 5208
BspMAI CTGCAG 3 cut(s) 1993, 2143, 4231
BspMI ACCTGC 3 cut(s) 3206, 4927, 5239
BspQI GCTCTTC 1 cut(s) 4401
BspT104I TTCGAA 2 cut(s) 3544, 3700
BspT107I GGYRCC 1 cut(s) 5206
BspTI CTTAAG 1 cut(s) 2360
BspTNI GGTCTC 3 cut(s) 1280, 4016, 5295
BsrDI GCAATG 7 cut(s) 333, 1812, 2285, 3097, 3747, 4479, 4909
BsrFI RCCGGY 3 cut(s) 63, 942, 1479
BsrGI TGTACA 1 cut(s) 5329
BsrI ACTGG 6 cut(s) 501, 2546, 3330, 4115, 4340, 4438
BssAI RCCGGY 3 cut(s) 63, 942, 1479
BssNAI GTATAC 1 cut(s) 1398
BssSI CACGAG 1 cut(s) 3417
Bst1107I GTATAC 1 cut(s) 1398
Bst2BI CACGAG 1 cut(s) 3417
Bst2UI CCWGG 5 cut(s) 588, 3455, 4520, 4608, 5061
Bst6I CTCTTC 5 cut(s) 36, 45, 4389, 4401, 4914
BstAFI CTTAAG 1 cut(s) 2360
BstAPI GCANNNNNTGC 6 cut(s) 908, 1883, 2120, 3221, 3515, 3851
BstAUI TGTACA 1 cut(s) 5329
BstBAI YACGTR 1 cut(s) 3832
BstBI TTCGAA 2 cut(s) 3544, 3700
BstDSI CCRYGG 6 cut(s) 690, 1940, 2589, 2646, 3519, 5069
BstEII GGTNACC 1 cut(s) 4244
BstENI CCTNNNNNAGG 1 cut(s) 2071
BstF5I GGATG 9 cut(s) 1227, 2432, 2904, 3304, 3425, 3450, 3529, 3955, 4292
BstFNI CGCG 2 cut(s) 615, 2438
BstHHI GCGC 2 cut(s) 1433, 3414
BstMAI GTCTC 7 cut(s) 1280, 1373, 1608, 2219, 3530, 4016, 5295
BstMCI CGRYCG 2 cut(s) 64, 3897
BstNI CCWGG 5 cut(s) 588, 3455, 4520, 4608, 5061
BstNSI RCATGY 8 cut(s) 1621, 1829, 1862, 3790, 3845, 4170, 4740, 5039
BstPI GGTNACC 1 cut(s) 4244
BstSCI CCNGG 6 cut(s) 586, 2978, 3453, 4518, 4606, 5059
BstSFI CTRYAG 4 cut(s) 1797, 1989, 2139, 4227
BstSLI GKGCMC 1 cut(s) 1103
BstUI CGCG 2 cut(s) 615, 2438
BstV2I GAAGAC 1 cut(s) 3018
BstX2I RGATCY 7 cut(s) 669, 1372, 1892, 3118, 3139, 3398, 3643
BstXI CCANNNNNNTGG 1 cut(s) 697
BstYI RGATCY 7 cut(s) 669, 1372, 1892, 3118, 3139, 3398, 3643
BstZ17I GTATAC 1 cut(s) 1398
Bsu15I ATCGAT 2 cut(s) 1080, 4218
BsuI GTATCC 2 cut(s) 3852, 5404
BsuTUI ATCGAT 2 cut(s) 1080, 4218
BtgI CCRYGG 6 cut(s) 690, 1940, 2589, 2646, 3519, 5069
BtsCI GGATG 9 cut(s) 1227, 2432, 2904, 3304, 3425, 3450, 3529, 3955, 4292
BtsI GCAGTG 4 cut(s) 989, 1137, 1335, 4224
BveI ACCTGC 3 cut(s) 3206, 4927, 5239
CaiI CAGNNNCTG 2 cut(s) 2552, 2884
CfoI GCGC 2 cut(s) 1433, 3414
Cfr10I RCCGGY 3 cut(s) 63, 942, 1479
Cfr13I GGNCC 9 cut(s) 700, 878, 1099, 1100, 2251, 3243, 3364, 3457, 3814
ClaI ATCGAT 2 cut(s) 1080, 4218
CseI GACGC 1 cut(s) 1250
Csp6I GTAC 9 cut(s) 724, 1364, 2733, 3072, 3274, 4175, 5330, 5343, 5359
CspAI ACCGGT 1 cut(s) 63
CviQI GTAC 9 cut(s) 724, 1364, 2733, 3072, 3274, 4175, 5330, 5343, 5359
DraI TTTAAA 2 cut(s) 1066, 4182
DraIII CACNNNGTG 1 cut(s) 5203
DriI GACNNNNNGTC 1 cut(s) 5308
EaeI YGGCCR 2 cut(s) 2407, 2794
Eam1104I CTCTTC 5 cut(s) 36, 45, 4389, 4401, 4914
Eam1105I GACNNNNNGTC 1 cut(s) 5308
EarI CTCTTC 5 cut(s) 36, 45, 4389, 4401, 4914
EciI GGCGGA 2 cut(s) 20, 154
Ecl136II GAGCTC 1 cut(s) 709
Eco147I AGGCCT 2 cut(s) 1545, 4301
Eco24I GRGCYC 3 cut(s) 711, 1103, 1354
Eco31I GGTCTC 3 cut(s) 1280, 4016, 5295
Eco32I GATATC 3 cut(s) 2497, 3448, 4322
Eco47I GGWCC 5 cut(s) 878, 2251, 3243, 3364, 3814
Eco53kI GAGCTC 1 cut(s) 709
Eco57I CTGAAG 7 cut(s) 1145, 1670, 3030, 3039, 3248, 4716, 5250
Eco88I CYCGRG 2 cut(s) 317, 5440
Eco91I GGTNACC 1 cut(s) 4244
EcoICRI GAGCTC 1 cut(s) 709
EcoNI CCTNNNNNAGG 1 cut(s) 2071
EcoO109I RGGNCCY 1 cut(s) 1100
EcoO65I GGTNACC 1 cut(s) 4244
EcoRI GAATTC 2 cut(s) 1046, 3166
EcoRII CCWGG 5 cut(s) 586, 3453, 4518, 4606, 5059
EcoRV GATATC 3 cut(s) 2497, 3448, 4322
EcoT22I ATGCAT 6 cut(s) 1276, 1879, 2929, 4168, 5037, 5228
EcoT38I GRGCYC 3 cut(s) 711, 1103, 1354
FalI AAGNNNNNCTT 6 cut(s) 895, 927, 3221, 3253, 4995, 5027
FaqI GGGAC 2 cut(s) 1187, 3123
FauI CCCGC 2 cut(s) 83, 591
FauNDI CATATG 2 cut(s) 1837, 3314
FbaI TGATCA 2 cut(s) 3469, 5283
FblI GTMKAC 1 cut(s) 1397
FokI GGATG 9 cut(s) 1234, 2419, 2911, 3311, 3412, 3437, 3536, 3962, 4279
FriOI GRGCYC 3 cut(s) 711, 1103, 1354
GlaI GCGC 2 cut(s) 1432, 3413
GsaI CCCAGC 1 cut(s) 3858
GsuI CTGGAG 7 cut(s) 819, 882, 1162, 1397, 2013, 2477, 4421
HapII CCGG 6 cut(s) 64, 172, 943, 1480, 2979, 3460
HgaI GACGC 1 cut(s) 1250
HhaI GCGC 2 cut(s) 1433, 3414
Hin6I GCGC 2 cut(s) 1431, 3412
HinP1I GCGC 2 cut(s) 1431, 3412
HincII GTYRAC 1 cut(s) 5043
HindII GTYRAC 1 cut(s) 5043
HindIII AAGCTT 5 cut(s) 392, 1682, 2708, 3002, 3728
HpaII CCGG 6 cut(s) 64, 172, 943, 1480, 2979, 3460
Hpy166II GTNNAC 8 cut(s) 205, 502, 1398, 2000, 2962, 3761, 4424, 5043
Hpy8I GTNNAC 8 cut(s) 205, 502, 1398, 2000, 2962, 3761, 4424, 5043
Hpy99I CGWCG 1 cut(s) 95
HpyCH4IV ACGT 6 cut(s) 522, 1703, 1921, 1996, 3831, 4426
HpySE526I ACGT 6 cut(s) 522, 1703, 1921, 1996, 3831, 4426
HspAI GCGC 2 cut(s) 1431, 3412
Kpn2I TCCGGA 1 cut(s) 171
Ksp22I TGATCA 2 cut(s) 3469, 5283
LguI GCTCTTC 1 cut(s) 4401
MaeII ACGT 6 cut(s) 522, 1703, 1921, 1996, 3831, 4426
MfeI CAATTG 6 cut(s) 597, 1887, 3968, 3989, 4647, 5313
MflI RGATCY 7 cut(s) 669, 1372, 1892, 3118, 3139, 3398, 3643
MhlI GDGCHC 3 cut(s) 711, 1103, 1354
MlsI TGGCCA 2 cut(s) 2409, 2796
MluNI TGGCCA 2 cut(s) 2409, 2796
MmeI TCCRAC 3 cut(s) 123, 130, 180
Mox20I TGGCCA 2 cut(s) 2409, 2796
Mph1103I ATGCAT 6 cut(s) 1276, 1879, 2929, 4168, 5037, 5228
MroI TCCGGA 1 cut(s) 171
MroXI GAANNNNTTC 1 cut(s) 3024
MscI TGGCCA 2 cut(s) 2409, 2796
MslI CAYNNNNRTG 7 cut(s) 1256, 2415, 2437, 2651, 3313, 4242, 4988
Msp20I TGGCCA 2 cut(s) 2409, 2796
MspA1I CMGCKG 4 cut(s) 1219, 1664, 2033, 2510
MspCI CTTAAG 1 cut(s) 2360
MspI CCGG 6 cut(s) 64, 172, 943, 1480, 2979, 3460
MspR9I CCNGG 6 cut(s) 588, 2980, 3455, 4520, 4608, 5061
MunI CAATTG 6 cut(s) 597, 1887, 3968, 3989, 4647, 5313
Mva1269I GAATGC 6 cut(s) 306, 584, 3509, 4983, 5228, 5441
MvaI CCWGG 5 cut(s) 588, 3455, 4520, 4608, 5061
MvnI CGCG 2 cut(s) 615, 2438
NciI CCSGG 1 cut(s) 2980
NcoI CCATGG 6 cut(s) 690, 1940, 2589, 2646, 3519, 5069
NdeI CATATG 2 cut(s) 1837, 3314
NlaIV GGNNCC 7 cut(s) 702, 1101, 1351, 2977, 3975, 4941, 5208
NsiI ATGCAT 6 cut(s) 1276, 1879, 2929, 4168, 5037, 5228
NspI RCATGY 8 cut(s) 1621, 1829, 1862, 3790, 3845, 4170, 4740, 5039
NspV TTCGAA 2 cut(s) 3544, 3700
OliI CACNNNNGTG 1 cut(s) 2415
PaeI GCATGC 4 cut(s) 1862, 3790, 3845, 4740
PaeR7I CTCGAG 1 cut(s) 317
PaqCI CACCTGC 1 cut(s) 3206
PceI AGGCCT 2 cut(s) 1545, 4301
PciI ACATGT 1 cut(s) 1617
PciSI GCTCTTC 1 cut(s) 4401
PcsI WCGNNNNNNNCGW 2 cut(s) 87, 528
PctI GAATGC 6 cut(s) 306, 584, 3509, 4983, 5228, 5441
PdmI GAANNNNTTC 1 cut(s) 3024
PfeI GAWTC 9 cut(s) 506, 815, 884, 1154, 1425, 2563, 3335, 4553, 4603
PflMI CCANNNNNTGG 1 cut(s) 3640
PfoI TCCNGGA 1 cut(s) 2978
PinAI ACCGGT 1 cut(s) 63
Ple19I CGATCG 1 cut(s) 3897
Ppu21I YACGTR 1 cut(s) 3832
PscI ACATGT 1 cut(s) 1617
PsiI TTATAA 1 cut(s) 3129
Psp124BI GAGCTC 1 cut(s) 711
Psp1406I AACGTT 1 cut(s) 522
Psp6I CCWGG 5 cut(s) 586, 3453, 4518, 4606, 5059
PspEI GGTNACC 1 cut(s) 4244
PspFI CCCAGC 1 cut(s) 3854
PspGI CCWGG 5 cut(s) 586, 3453, 4518, 4606, 5059
PspN4I GGNNCC 7 cut(s) 702, 1101, 1351, 2977, 3975, 4941, 5208
PspOMI GGGCCC 1 cut(s) 1099
PspPI GGNCC 9 cut(s) 700, 878, 1099, 1100, 2251, 3243, 3364, 3457, 3814
PstI CTGCAG 3 cut(s) 1993, 2143, 4231
PstNI CAGNNNCTG 2 cut(s) 2552, 2884
PsuI RGATCY 7 cut(s) 669, 1372, 1892, 3118, 3139, 3398, 3643
PvuI CGATCG 1 cut(s) 3897
PvuII CAGCTG 2 cut(s) 1664, 2033
RsaI GTAC 9 cut(s) 725, 1365, 2734, 3073, 3275, 4176, 5331, 5344, 5360
RsaNI GTAC 9 cut(s) 724, 1364, 2733, 3072, 3274, 4175, 5330, 5343, 5359
RseI CAYNNNNRTG 7 cut(s) 1256, 2415, 2437, 2651, 3313, 4242, 4988
SacI GAGCTC 1 cut(s) 711
SapI GCTCTTC 1 cut(s) 4401
Sau96I GGNCC 9 cut(s) 700, 878, 1099, 1100, 2251, 3243, 3364, 3457, 3814
ScaI AGTACT 2 cut(s) 1365, 4176
ScrFI CCNGG 6 cut(s) 588, 2980, 3455, 4520, 4608, 5061
SduI GDGCHC 3 cut(s) 711, 1103, 1354
SfcI CTRYAG 4 cut(s) 1797, 1989, 2139, 4227
Sfr274I CTCGAG 1 cut(s) 317
SfuI TTCGAA 2 cut(s) 3544, 3700
SinI GGWCC 5 cut(s) 878, 2251, 3243, 3364, 3814
SlaI CTCGAG 1 cut(s) 317
SmiMI CAYNNNNRTG 7 cut(s) 1256, 2415, 2437, 2651, 3313, 4242, 4988
SpeI ACTAGT 1 cut(s) 4051
SphI GCATGC 4 cut(s) 1862, 3790, 3845, 4740
SseBI AGGCCT 2 cut(s) 1545, 4301
SspI AATATT 3 cut(s) 1295, 2449, 2466
SstI GAGCTC 1 cut(s) 711
StuI AGGCCT 2 cut(s) 1545, 4301
StyD4I CCNGG 6 cut(s) 586, 2978, 3453, 4518, 4606, 5059
TaiI ACGT 6 cut(s) 525, 1706, 1924, 1999, 3834, 4429
TaqII GACCGA 2 cut(s) 4263, 5298
TatI WGTACW 5 cut(s) 1363, 2732, 3071, 4174, 5329
TauI GCSGC 2 cut(s) 959, 2510
TfiI GAWTC 9 cut(s) 506, 815, 884, 1154, 1425, 2563, 3335, 4553, 4603
TspGWI ACGGA 2 cut(s) 2124, 3503
Van91I CCANNNNNTGG 1 cut(s) 3640
Vha464I CTTAAG 1 cut(s) 2360
VpaK11BI GGWCC 5 cut(s) 878, 2251, 3243, 3364, 3814
XagI CCTNNNNNAGG 1 cut(s) 2071
XbaI TCTAGA 2 cut(s) 887, 2153
XceI RCATGY 8 cut(s) 1621, 1829, 1862, 3790, 3845, 4170, 4740, 5039
XhoI CTCGAG 1 cut(s) 317
XmiI GTMKAC 1 cut(s) 1397
XmnI GAANNNNTTC 1 cut(s) 3024
ZrmI AGTACT 2 cut(s) 1365, 4176
Zsp2I ATGCAT 6 cut(s) 1276, 1879, 2929, 4168, 5037, 5228
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.