Rw5G032870

Proteasome-associated protein ECM29 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0006358
Physical Location & Seq
Forward (+)
55723474 .. 55748212
24739 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G032870.1

Sequence Viewer

Length: 5451 bp
ATGGCGGAAACATCATCGGCTGTATCATCTTCCAAATCAGATGAAGAGAAAGAAGAGATGCTCGACCGGTTGCTCACCCGCCTCGCTCTCTGCGACGATTCCAACCTCCAACCTCTACTCTCCAAGCTCCTCCCCTATACTATTTCCTCCCTTTCTTCCAACTCCTCCGCCGTCCGGAACAAGGTGCTAGAAATACTGAGTCATGTGAACAAGCGAGTGAAGCATCAACCTGAGATTGGTTTGCCCTTGACTGAATTATGGACTATATACTCGGAAGCTCATTCTGCTCCAATGGTCAGGAATTTTTGCATTCTCTACCTCGAGATGGCAATGGATCGTGCCGATAGAAAGGAGAAAGAAAATTTGTCACCCATGCTTCTGTTAGGGGTTTCCAAGCTTCCTCAACAGCACCAAGAGATTATACTCAGACTTGTTATAAAGGTCATTGGTGAATGTCACACAAACGGAATAGATGGAGAAGTTGCTGCAAAGTATGGACTGATAAATGATTCTCAAGACAGAAACGTTTTTATCGAATTTTGCCTTCATACAATCTTATATCAGCCGTCATCTCAAAGAGATTGCCCGCCAGGACTTTCAATTGCTCAAGCCAACCGCGTAACAGCTAGACAATCATTGAACAGTGAAATGCTTCTAACAAGAAAGTTAGGGATCTTGAATGTTGTTGAGGCCATGGTGCTGGCCGCTGAGCTCGTTTATCCTTTGTACCTGACTGCCTCCATAGATTGTCAAGAGCTTGTCGTTAAGAGAGGAGAGGAGCTTGTAAAGAAGAAGGCTGCTGGAGCTAATTTAGATGATTCAATCCTCATAAACAGACTTTTTTTGTTATTTAATGGAACTGCTACATCTCAAAATGTTGGTCCTGAATCTAGAGTCACTCCAGCAAGTCCTGCTTTGAAAGGAAAAATGATGTCTATTTTCTGCCGGTCTATTACAGCCGCTAACAGTTTCCCTTCAACCTTGCAGTGCATTTTTGGTTGCATATATGGAAGTGATACCACATCAAGGTTGAAGCAGTTGGGAATGGAATTCACTGTCTGGGTATTTAAACATTCCATAATCGATCAACTTAAACTTATGGGCCCTGTCATTCTGAGTGGAATACTGAAGTCACTTGACACTGCTTCAAGTTCAGAATCAGATGCTACTCATAGGGACTCCAGAACTTTCGCTTATCAAGCTATTGGATTGCTTGCACAGCGGATGCCTCAGCTTTTCAGAGATACGACTGACATGGCTGTGCGTCTTTTTGATGCATTGAAGGTAGAGACCCAATATTTTCGCCTCAGTATTCAAGAAGCAACAAATTCTCTTGCCACTGCATATAAGGGAGCCCCCTCAACAGTACTAAAGGATCTGGAGACTCTTTTGCTCAAGTATACTCAAGAGGAACAAAGTGAAGTAAGATTCTGCGCTATAAGATGGGTGACTTCTTTATTTGACTTACAACATTGTCCAAGCCGGTATATCTGTATGCTTGGGGCTGCTGATATTAAATTGGATATAAGGGAGATTGCATTTGAAGGCCTTTTTCCTATGAAAGATGATGGGTCAAGTATGAGTAAGATACAAGAGCTACATTACCCCAAACTTGGAGACATGTTAGATTATATTCTTTCACAGCAGCCAAACTTGTCAGAACCAGCTGAGATACGGGAGCAGAAGCTTCAGTTTCCCTCAAAAACGTATCTAGTTATTATTGAATTTTTGTTGAAGTGCTTTGAGTCAGAATTGGAGCATAACAATTCAATTAAAGGATCAACTCAGTTTCACTCGTCTGTAGAAGCAATGTGCTTACTTCTGGAACATGCTATGGCATATGAAGGGTCTGTTGAGTTGCATGCCAAGGCTTCCAATGCATTGATTGCAATTGGATCTCATATTCCAGAGCTTGTAGCTTCACGTTATGCAAAAAAAGTTCAATGGCTAAAACAATTATTGAGTCATATTGATTTAGATACCCGTGAAGCTGCAGCACGTTTACTTGGTATTGCTTCCTCCGTTCTTCCTCCAGCTGCTTCATGTGATCTTATTTCTGAAATAATTGCCTCAGTAAGGGGAATACATAAGTTAAGGTTTGAGGTTCAACACGGAGCATTATGTGCATTAGGTTATGTAACTGCAGATTGTATCTCTAGAAAATCAGCTATCCCAGAGGCATTATTTCAAACTACCCTAAAGTTACTTGTCGATGTTGTTAATTCTGAGACTGCGACACTAGCATCAGTAGCGGTCCAAGCGTTAGGTCATATTGGACTGATCGTTGCATTGCCTTCACTTATTATTGAGTCTTGTTCAGTTGATATTCTGGTGCTTTTACAAGAAAGATTGAGAAAGCTACTTAAGGGTGATGATACCAAAGCAATTCAGAAAATTATTATATCCATTGGCCACATCTGTGTCAAGGAAACATCATCCGCGTGTCTTAATATTGCTCTGGAGTTAATATTCAGTCTTTCCCGATCTAAGGTTGAGGATATCCTCTTTGCCGCTGGCGAGGCACTGTCGTTTTTGTGGGGTGGTGTTCCGGTGACTGCTGATTTGATTCTCAGAACTAATTATTCACTCTCCATGGCTTCTAAGTTTCTCATGGGAGATGCGAGTTTATCTCTCTCAAAACATAGCCCCATGGAAATGAATGAAGCTAATAAAGATCGTGATGACATGGTTCGAGAAGCAATTACTAAAAAGCTTTTTGACGATCTTCTATACAGTACAAGAAAAGAAGATCGCTGTGCTGGAACTGTATGGCTATTATCAATTACCATGTATTGTGGCCATCAACCAGCTATTCAGAAAATGCTGCCAGAAATTCAGGAGGCTTTTTCACACCTTCTTGGTGAACAGAATGAACTCACGCAGGAGCTGGCCTCACAAGGGATGAGCGTTGTGTATGAAATTGGTGATGCATCAATGAAAGGAAACTTGGTAAATGCACTTGTAAACACTCTGACAGGTTCCGGGAAGAAAAAAAGAGCTATAAAGCTTGCTGAAGACTCTGAAGTTTTTCAAGAGGGAGTTATTGGTGAAGGTCTTAGTGGAGGCAAACTCAGTACATACAAGGAGCTGTGCAATGTAGCGAATGAGATGGGACAACCAGATCTGATTTATAAGTTTATGGATCTGGCTAATTATCAAACTTCTCTGAATTCAAAGAGGGGTGCTGCCTTTGGTTTTTCAAAGATAGCAAAGCAAGCAGGAGATGCTCTGAAGCCACACTTGCGGTCCTTAATTCCAAGGCTTGTTCGTTATCAGTACGATCCTGACAAAAATGTGCAGGATGCTATGTCACACATATGGAAGTCACTGGTGGAAGATTCAAAGAAAACCATTGATGAACACTTGGACCTCATTATTGATGACTTGTTAATACAATGTGGATCTAGGCTTTGGCGCACACGAGAGGCATCCTGTCTTGCCCTTGCAGATATCATCCAGGGCCGGAAGTTTGATCAGGTTGGGAAGCATTTAAGGAAGTTATGGTCAGCAGCATTCCGTGCCATGGATGATATTAAAGAGACTGTTCGAAATTCTGGCGACAAGTTATGCCGTGTTTTAACTTCATTGACGGTGAGGCTTTCTGATGTCACCCTAACTGATGTATCAGATGCAAGCCAAACAATGGATCTTGTTCTGCCTCTCTTGCTTACAGAAGGCATACTAAGTAAAGTTGATAGTATTCGAAAGGCATCTATAGAGGTTGTTATGAAGCTTGCAAAGGGGGCTGGTATTGCAATTCGTCCACATTTATCTGATCTAGTTTGTTGCATGCTGGAAAGTTTATCGAGCCTTGAGGACCAAGGGCTTAATTACGTGGAGTTGCATGCAGCAAATGCTGGGATACAGACAGAGAAGCTCGAAAGTTTGAGAATTTCGATTGCAAAAGGCTCTCCCATGTGGGAAACTCTTGATCTCTGCATCAAAGTTGTGGATGCAGGATCATTAGATCAATTGGTTCCTCGTCTTGGTCAATTGGTTCGATCTGGGGTTGGTCTCAATACAAGGGTTGGTGTTGCCAGCTTTATTACCTTACTAGTTCAAGAAGTTGGTGTTGAAATCAAACCATATACGAGCAAGCTGCTGAGGCTGTTGTTTCCAGTTGTGAAGGACGAAAAAAGTGCTGCATCAAAACGAGCCTTTGCTGATGCATGTGCAGTACTTTTAAAACATACAGTAGCCTCTCAGGCCGAAAAGTTGATCGATGACACTGCAGCTTTGCATATTGGTGACCGAAATGCTCAAGTGGCTTGTGCGGTTTTATTGAAAAGCTATTCATCCAAGGCCTCAGACATTTTGGATGGATATATTGCAGCTATACTTCCAGTAATTTTTATCTCAAGATTTGACGATGACAAGTATGTTTCTGGCCTGTTTGAAGAGTTGTGGGAAGAGCATACAAGTAGTGAACGTGTAGCCCTCCAGTTATACTTGGCCGAAATTGTTTCTCTTATTTGTGAGAGCATTGCAACATCTTCTTGGGCAAGTAAAAAGAAGTCAGCCCAGGCCATTTCCAAACTCAGTGAAGTTTTGGGTGAATCTCTGGCTTCTTACTATAATGTCTTGCTCAAATCTCTTATGAAAGAGATTCCTGGTCGGTTATGGGAGGGAAAGGAAGCTCTATTGTATTCAATTGCTGCACTTTCTGTGTCTTGTCATAAAGCAATTTCAACTGATGATCCTCATACTCTGAATGAAGTTTTGAGGGTATTATCTTCAGCATGCACGAAAAAGGCAAAGAAATATCGTGAAGCAGCTCTATCCTGTCTTGAACAGGTTGTAAAAGCCTTTGGTAATCAAGAGTTCTTTAATGAGGCATTTCCGTTGTTATACGATATGTGTAATGCTTCCACTCTCGGTGTTTCTGGGAAAGCAACTTTGGCTGGCAATGGTGCTAAAGCAGAAGAGGATCAAATTGAGCAGGTTCCTGTTTCACATGAGAAAATCTTGGACTGCATGACCGCTTGCATTCATGTGGCTAATATAAAAGATATTCTTGAACAACAGAAGAACTTGATGCATGTGTTGACTACTGCTCTGTCACCTGGATTTCCATGGACAGTCAAAATATCTGCCCTTTCATTGATAAAGGAACTTGTTTCAAGTGTCCAGAAGGTTCTGGGTGACTCCAAAGAAACTTATGCTCATGCCAACATAATTTTGTTAGTTCAGGAGTTGTTTCACTCAGTGGCACCTCAAGTTGTCGAATGCATAAGCACAATTAAAGTTGGGCAGGTTCATGTAACTGCTTCAGAGTGCCTTCTAGTGATCATCACGCTTTATGGAGACCTTCGGTCAATTGACTCTACTAATGTACAATTTGAGGGTACGCTTCTGCATCTGTACGAAGTGGAGAAAAATGGCGAAGCAAAGTCCTTGTTGAAAAAATGTATGGATACCCTTGAAAACTTAAAACTAGAATGCCCGAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ecm29

Protein Analysis

1816

Amino Acids

200.07

Weight (kDa)

6.04

Isoelectric Point (pI)

40.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ECM29_N PF13001 21 - 501 3.1e-132 Proteasome component ECM29, N-terminal
ARM_ECM29 PF23702 626 - 756 1.9e-12 ECM29, ARM-like repeats
HEAT_GCN1 PF23271 1067 - 1192 9.8e-07 Stalled ribosome sensor GCN1-like, HEAT repeats
HEAT_ECM29 PF24492 1253 - 1412 3.7e-47 Proteasome adapter and scaffold protein ECM29, HEAT-repeat domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 437, 3132
Acc36I ACCTGC 2 cut(s) 4930, 5242
AccB1I GGYRCC 1 cut(s) 5209
AccB7I CCANNNNNTGG 1 cut(s) 3643
AccI GTMKAC 1 cut(s) 1400
AccII CGCG 2 cut(s) 618, 2441
AccIII TCCGGA 1 cut(s) 174
AclI AACGTT 1 cut(s) 525
AcoI YGGCCR 4 cut(s) 702, 2410, 2797, 4452
AcuI CTGAAG 7 cut(s) 1148, 1673, 3033, 3042, 3251, 4719, 5253
AdeI CACNNNGTG 1 cut(s) 5206
AfaI GTAC 9 cut(s) 728, 1368, 2737, 3076, 3278, 4179, 5334, 5347, 5363
AflII CTTAAG 1 cut(s) 2363
AflIII ACRYGT 2 cut(s) 1620, 4429
AgeI ACCGGT 1 cut(s) 66
AhdI GACNNNNNGTC 1 cut(s) 5311
AhlI ACTAGT 1 cut(s) 4054
AjnI CCWGG 5 cut(s) 589, 3456, 4521, 4609, 5062
AjuI GAANNNNNNNTTGG 4 cut(s) 2930, 2962, 4880, 4912
AleI CACNNNNGTG 1 cut(s) 2418
Alw21I GWGCWC 1 cut(s) 714
Alw26I GTCTC 7 cut(s) 1283, 1376, 1611, 2222, 3533, 4019, 5298
AlwNI CAGNNNCTG 1 cut(s) 2887
Ama87I CYCGRG 2 cut(s) 320, 5443
Aor13HI TCCGGA 1 cut(s) 174
ApaI GGGCCC 1 cut(s) 1106
ArsI GACNNNNNNTTYG 4 cut(s) 865, 897, 2545, 2577
AsiGI ACCGGT 1 cut(s) 66
Asp700I GAANNNNTTC 2 cut(s) 651, 3027
AspLEI GCGC 2 cut(s) 1436, 3417
AspS9I GGNCC 8 cut(s) 881, 1102, 1103, 2254, 3246, 3367, 3460, 3817
AsuC2I CCSGG 1 cut(s) 2983
AsuII TTCGAA 2 cut(s) 3547, 3703
AvaI CYCGRG 2 cut(s) 320, 5443
AvaII GGWCC 5 cut(s) 881, 2254, 3246, 3367, 3817
BaeGI GKGCMC 1 cut(s) 1106
BaeI ACNNNNGTAYC 2 cut(s) 3606, 3639
BalI TGGCCA 2 cut(s) 2412, 2799
BanI GGYRCC 1 cut(s) 5209
BanII GRGCYC 3 cut(s) 714, 1106, 1357
BauI CACGAG 1 cut(s) 3420
BbsI GAAGAC 1 cut(s) 3021
Bbv12I GWGCWC 1 cut(s) 714
BbvCI CCTCAGC 2 cut(s) 1230, 4103
BccI CCATC 7 cut(s) 319, 467, 1437, 1562, 2808, 3103, 4313
BceAI ACGGC 3 cut(s) 155, 550, 3555
BcgI CGANNNNNNTGC 6 cut(s) 4081, 4115, 4121, 4155, 4211, 4245
BciT130I CCWGG 5 cut(s) 591, 3458, 4523, 4611, 5064
BciVI GTATCC 2 cut(s) 3855, 5407
BclI TGATCA 2 cut(s) 3472, 5286
BcnI CCSGG 1 cut(s) 2983
BcoDI GTCTC 7 cut(s) 1283, 1376, 1611, 2222, 3533, 4019, 5298
BcuI ACTAGT 1 cut(s) 4054
BfmI CTRYAG 5 cut(s) 1800, 1992, 2142, 3714, 4230
BfrI CTTAAG 1 cut(s) 2363
BfuAI ACCTGC 2 cut(s) 4930, 5242
BfuI GTATCC 2 cut(s) 3855, 5407
BglI GCCNNNNNGGC 1 cut(s) 4205
BglII AGATCT 1 cut(s) 3121
BlpI GCTNAGC 1 cut(s) 708
BmcAI AGTACT 2 cut(s) 1368, 4179
Bme1390I CCNGG 6 cut(s) 591, 2983, 3458, 4523, 4611, 5064
Bme18I GGWCC 5 cut(s) 881, 2254, 3246, 3367, 3817
BmeRI GACNNNNNGTC 1 cut(s) 5311
BmeT110I CYCGRG 2 cut(s) 320, 5443
BmgT120I GGNCC 8 cut(s) 881, 1102, 1103, 2254, 3246, 3367, 3460, 3817
BmiI GGNNCC 6 cut(s) 1104, 1354, 2980, 3978, 4944, 5211
BmrFI CCNGG 6 cut(s) 591, 2983, 3458, 4523, 4611, 5064
BpiI GAAGAC 1 cut(s) 3021
BplI GAGNNNNNCTC 6 cut(s) 2614, 2646, 2876, 2908, 3054, 3086
BpmI CTGGAG 7 cut(s) 822, 885, 1165, 1400, 2016, 2480, 4424
Bpu10I CCTNAGC 2 cut(s) 1230, 4103
Bpu1102I GCTNAGC 1 cut(s) 708
Bpu14I TTCGAA 2 cut(s) 3547, 3703
BpuEI CTTGAG 8 cut(s) 498, 591, 1379, 1389, 3833, 4245, 4342, 5199
BpuMI CCSGG 1 cut(s) 2983
Bsa29I ATCGAT 2 cut(s) 1083, 4221
BsaAI YACGTR 1 cut(s) 3835
BsaI GGTCTC 3 cut(s) 1283, 4019, 5298
BsaWI WCCGGW 3 cut(s) 66, 174, 2548
Bse118I RCCGGY 3 cut(s) 66, 945, 1482
Bse1I ACTGG 4 cut(s) 3333, 4118, 4343, 4441
Bse3DI GCAATG 6 cut(s) 336, 1815, 2288, 3100, 4482, 4912
BseAI TCCGGA 1 cut(s) 174
BseBI CCWGG 5 cut(s) 591, 3458, 4523, 4611, 5064
BseCI ATCGAT 2 cut(s) 1083, 4221
BseMI GCAATG 6 cut(s) 336, 1815, 2288, 3100, 4482, 4912
BseNI ACTGG 4 cut(s) 3333, 4118, 4343, 4441
BseRI GAGGAG 4 cut(s) 119, 154, 786, 791
BseSI GKGCMC 1 cut(s) 1106
BseYI CCCAGC 1 cut(s) 3857
BsgI GTGCAG 3 cut(s) 3317, 4194, 4641
Bsh1236I CGCG 2 cut(s) 618, 2441
Bsh1285I CGRYCG 1 cut(s) 67
BshNI GGYRCC 1 cut(s) 5209
BshTI ACCGGT 1 cut(s) 66
BshVI ATCGAT 2 cut(s) 1083, 4221
BsiEI CGRYCG 1 cut(s) 67
BsiHKAI GWGCWC 1 cut(s) 714
BsiHKCI CYCGRG 2 cut(s) 320, 5443
BsiSI CCGG 7 cut(s) 67, 175, 946, 1483, 2549, 2982, 3463
BslFI GGGAC 2 cut(s) 1190, 3126
BsmAI GTCTC 7 cut(s) 1283, 1376, 1611, 2222, 3533, 4019, 5298
BsmFI GGGAC 2 cut(s) 1190, 3126
BsmI GAATGC 5 cut(s) 309, 3512, 4986, 5231, 5444
Bso31I GGTCTC 3 cut(s) 1283, 4019, 5298
BsoBI CYCGRG 2 cut(s) 320, 5443
Bsp119I TTCGAA 2 cut(s) 3547, 3703
Bsp120I GGGCCC 1 cut(s) 1102
Bsp1286I GDGCHC 3 cut(s) 714, 1106, 1357
Bsp13I TCCGGA 1 cut(s) 174
Bsp1407I TGTACA 1 cut(s) 5332
Bsp1720I GCTNAGC 1 cut(s) 708
Bsp19I CCATGG 5 cut(s) 693, 2592, 2649, 3522, 5072
BspDI ATCGAT 2 cut(s) 1083, 4221
BspEI TCCGGA 1 cut(s) 174
BspFNI CGCG 2 cut(s) 618, 2441
BspLI GGNNCC 6 cut(s) 1104, 1354, 2980, 3978, 4944, 5211
BspMAI CTGCAG 3 cut(s) 1996, 2146, 4234
BspMI ACCTGC 2 cut(s) 4930, 5242
BspQI GCTCTTC 1 cut(s) 4404
BspT104I TTCGAA 2 cut(s) 3547, 3703
BspT107I GGYRCC 1 cut(s) 5209
BspTI CTTAAG 1 cut(s) 2363
BspTNI GGTCTC 3 cut(s) 1283, 4019, 5298
BsrDI GCAATG 6 cut(s) 336, 1815, 2288, 3100, 4482, 4912
BsrFI RCCGGY 3 cut(s) 66, 945, 1482
BsrGI TGTACA 1 cut(s) 5332
BsrI ACTGG 4 cut(s) 3333, 4118, 4343, 4441
BssAI RCCGGY 3 cut(s) 66, 945, 1482
BssNAI GTATAC 1 cut(s) 1401
BssSI CACGAG 1 cut(s) 3420
BssT1I CCWWGG 9 cut(s) 693, 1866, 2592, 2649, 3257, 3522, 3820, 4299, 5072
Bst1107I GTATAC 1 cut(s) 1401
Bst2BI CACGAG 1 cut(s) 3420
Bst2UI CCWGG 5 cut(s) 591, 3458, 4523, 4611, 5064
Bst6I CTCTTC 5 cut(s) 39, 48, 4392, 4404, 4917
BstAFI CTTAAG 1 cut(s) 2363
BstAPI GCANNNNNTGC 6 cut(s) 911, 1886, 2123, 3224, 3518, 3854
BstAUI TGTACA 1 cut(s) 5332
BstBAI YACGTR 1 cut(s) 3835
BstBI TTCGAA 2 cut(s) 3547, 3703
BstDSI CCRYGG 5 cut(s) 693, 2592, 2649, 3522, 5072
BstEII GGTNACC 1 cut(s) 4247
BstENI CCTNNNNNAGG 1 cut(s) 2074
BstFNI CGCG 2 cut(s) 618, 2441
BstHHI GCGC 2 cut(s) 1436, 3417
BstMAI GTCTC 7 cut(s) 1283, 1376, 1611, 2222, 3533, 4019, 5298
BstMCI CGRYCG 1 cut(s) 67
BstNI CCWGG 5 cut(s) 591, 3458, 4523, 4611, 5064
BstNSI RCATGY 8 cut(s) 1624, 1832, 1865, 3793, 3848, 4173, 4743, 5042
BstPI GGTNACC 1 cut(s) 4247
BstSCI CCNGG 6 cut(s) 589, 2981, 3456, 4521, 4609, 5062
BstSFI CTRYAG 5 cut(s) 1800, 1992, 2142, 3714, 4230
BstSLI GKGCMC 1 cut(s) 1106
BstUI CGCG 2 cut(s) 618, 2441
BstV2I GAAGAC 1 cut(s) 3021
BstX2I RGATCY 7 cut(s) 672, 1375, 1895, 3121, 3142, 3401, 3646
BstXI CCANNNNNNTGG 1 cut(s) 700
BstYI RGATCY 7 cut(s) 672, 1375, 1895, 3121, 3142, 3401, 3646
BstZ17I GTATAC 1 cut(s) 1401
Bsu15I ATCGAT 2 cut(s) 1083, 4221
BsuI GTATCC 2 cut(s) 3855, 5407
BsuTUI ATCGAT 2 cut(s) 1083, 4221
BtgI CCRYGG 5 cut(s) 693, 2592, 2649, 3522, 5072
BtsI GCAGTG 4 cut(s) 992, 1140, 1338, 4227
BveI ACCTGC 2 cut(s) 4930, 5242
CaiI CAGNNNCTG 1 cut(s) 2887
CfoI GCGC 2 cut(s) 1436, 3417
Cfr10I RCCGGY 3 cut(s) 66, 945, 1482
Cfr13I GGNCC 8 cut(s) 881, 1102, 1103, 2254, 3246, 3367, 3460, 3817
ClaI ATCGAT 2 cut(s) 1083, 4221
CseI GACGC 1 cut(s) 1253
Csp6I GTAC 9 cut(s) 727, 1367, 2736, 3075, 3277, 4178, 5333, 5346, 5362
CspAI ACCGGT 1 cut(s) 66
CviQI GTAC 9 cut(s) 727, 1367, 2736, 3075, 3277, 4178, 5333, 5346, 5362
DraI TTTAAA 2 cut(s) 1069, 4185
DraIII CACNNNGTG 1 cut(s) 5206
DriI GACNNNNNGTC 1 cut(s) 5311
EaeI YGGCCR 4 cut(s) 702, 2410, 2797, 4452
Eam1104I CTCTTC 5 cut(s) 39, 48, 4392, 4404, 4917
Eam1105I GACNNNNNGTC 1 cut(s) 5311
EarI CTCTTC 5 cut(s) 39, 48, 4392, 4404, 4917
EciI GGCGGA 2 cut(s) 20, 157
Ecl136II GAGCTC 1 cut(s) 712
Eco130I CCWWGG 9 cut(s) 693, 1866, 2592, 2649, 3257, 3522, 3820, 4299, 5072
Eco147I AGGCCT 2 cut(s) 1548, 4304
Eco24I GRGCYC 3 cut(s) 714, 1106, 1357
Eco31I GGTCTC 3 cut(s) 1283, 4019, 5298
Eco32I GATATC 2 cut(s) 2500, 3451
Eco47I GGWCC 5 cut(s) 881, 2254, 3246, 3367, 3817
Eco53kI GAGCTC 1 cut(s) 712
Eco57I CTGAAG 7 cut(s) 1148, 1673, 3033, 3042, 3251, 4719, 5253
Eco88I CYCGRG 2 cut(s) 320, 5443
Eco91I GGTNACC 1 cut(s) 4247
EcoICRI GAGCTC 1 cut(s) 712
EcoNI CCTNNNNNAGG 1 cut(s) 2074
EcoO109I RGGNCCY 1 cut(s) 1103
EcoO65I GGTNACC 1 cut(s) 4247
EcoRI GAATTC 2 cut(s) 1049, 3169
EcoRII CCWGG 5 cut(s) 589, 3456, 4521, 4609, 5062
EcoRV GATATC 2 cut(s) 2500, 3451
EcoT14I CCWWGG 9 cut(s) 693, 1866, 2592, 2649, 3257, 3522, 3820, 4299, 5072
EcoT22I ATGCAT 6 cut(s) 1279, 1882, 2932, 4171, 5040, 5231
EcoT38I GRGCYC 3 cut(s) 714, 1106, 1357
ErhI CCWWGG 9 cut(s) 693, 1866, 2592, 2649, 3257, 3522, 3820, 4299, 5072
FalI AAGNNNNNCTT 6 cut(s) 898, 930, 3224, 3256, 4998, 5030
FaqI GGGAC 2 cut(s) 1190, 3126
FauI CCCGC 2 cut(s) 86, 594
FauNDI CATATG 2 cut(s) 1840, 3317
FbaI TGATCA 2 cut(s) 3472, 5286
FblI GTMKAC 1 cut(s) 1400
FriOI GRGCYC 3 cut(s) 714, 1106, 1357
GlaI GCGC 2 cut(s) 1435, 3416
GsaI CCCAGC 1 cut(s) 3861
GsuI CTGGAG 7 cut(s) 822, 885, 1165, 1400, 2016, 2480, 4424
HapII CCGG 7 cut(s) 67, 175, 946, 1483, 2549, 2982, 3463
HgaI GACGC 1 cut(s) 1253
HhaI GCGC 2 cut(s) 1436, 3417
Hin6I GCGC 2 cut(s) 1434, 3415
HinP1I GCGC 2 cut(s) 1434, 3415
HincII GTYRAC 1 cut(s) 5046
HindII GTYRAC 1 cut(s) 5046
HindIII AAGCTT 5 cut(s) 395, 1685, 2711, 3005, 3731
HpaII CCGG 7 cut(s) 67, 175, 946, 1483, 2549, 2982, 3463
Hpy166II GTNNAC 8 cut(s) 208, 1401, 2003, 2864, 2965, 3764, 4427, 5046
Hpy8I GTNNAC 8 cut(s) 208, 1401, 2003, 2864, 2965, 3764, 4427, 5046
Hpy99I CGWCG 1 cut(s) 98
HpyCH4IV ACGT 6 cut(s) 525, 1706, 1924, 1999, 3834, 4429
HpySE526I ACGT 6 cut(s) 525, 1706, 1924, 1999, 3834, 4429
HspAI GCGC 2 cut(s) 1434, 3415
Kpn2I TCCGGA 1 cut(s) 174
Ksp22I TGATCA 2 cut(s) 3472, 5286
LguI GCTCTTC 1 cut(s) 4404
MaeII ACGT 6 cut(s) 525, 1706, 1924, 1999, 3834, 4429
MfeI CAATTG 6 cut(s) 600, 1890, 3971, 3992, 4650, 5316
MflI RGATCY 7 cut(s) 672, 1375, 1895, 3121, 3142, 3401, 3646
MhlI GDGCHC 3 cut(s) 714, 1106, 1357
MlsI TGGCCA 2 cut(s) 2412, 2799
MluNI TGGCCA 2 cut(s) 2412, 2799
MmeI TCCRAC 3 cut(s) 126, 133, 183
Mox20I TGGCCA 2 cut(s) 2412, 2799
Mph1103I ATGCAT 6 cut(s) 1279, 1882, 2932, 4171, 5040, 5231
MroI TCCGGA 1 cut(s) 174
MroXI GAANNNNTTC 2 cut(s) 651, 3027
MscI TGGCCA 2 cut(s) 2412, 2799
MslI CAYNNNNRTG 7 cut(s) 1259, 2418, 2440, 2654, 3316, 4245, 4991
Msp20I TGGCCA 2 cut(s) 2412, 2799
MspA1I CMGCKG 5 cut(s) 707, 1222, 1667, 2036, 2513
MspCI CTTAAG 1 cut(s) 2363
MspI CCGG 7 cut(s) 67, 175, 946, 1483, 2549, 2982, 3463
MspR9I CCNGG 6 cut(s) 591, 2983, 3458, 4523, 4611, 5064
MunI CAATTG 6 cut(s) 600, 1890, 3971, 3992, 4650, 5316
Mva1269I GAATGC 5 cut(s) 309, 3512, 4986, 5231, 5444
MvaI CCWGG 5 cut(s) 591, 3458, 4523, 4611, 5064
MvnI CGCG 2 cut(s) 618, 2441
NciI CCSGG 1 cut(s) 2983
NcoI CCATGG 5 cut(s) 693, 2592, 2649, 3522, 5072
NdeI CATATG 2 cut(s) 1840, 3317
NlaIV GGNNCC 6 cut(s) 1104, 1354, 2980, 3978, 4944, 5211
NsiI ATGCAT 6 cut(s) 1279, 1882, 2932, 4171, 5040, 5231
NspI RCATGY 8 cut(s) 1624, 1832, 1865, 3793, 3848, 4173, 4743, 5042
NspV TTCGAA 2 cut(s) 3547, 3703
OliI CACNNNNGTG 1 cut(s) 2418
PaeI GCATGC 4 cut(s) 1865, 3793, 3848, 4743
PaeR7I CTCGAG 1 cut(s) 320
PceI AGGCCT 2 cut(s) 1548, 4304
PciI ACATGT 1 cut(s) 1620
PciSI GCTCTTC 1 cut(s) 4404
PcsI WCGNNNNNNNCGW 2 cut(s) 90, 531
PctI GAATGC 5 cut(s) 309, 3512, 4986, 5231, 5444
PdmI GAANNNNTTC 2 cut(s) 651, 3027
PflMI CCANNNNNTGG 1 cut(s) 3643
PfoI TCCNGGA 1 cut(s) 2981
PinAI ACCGGT 1 cut(s) 66
Ppu21I YACGTR 1 cut(s) 3835
PscI ACATGT 1 cut(s) 1620
PsiI TTATAA 2 cut(s) 437, 3132
Psp124BI GAGCTC 1 cut(s) 714
Psp1406I AACGTT 1 cut(s) 525
Psp6I CCWGG 5 cut(s) 589, 3456, 4521, 4609, 5062
PspEI GGTNACC 1 cut(s) 4247
PspFI CCCAGC 1 cut(s) 3857
PspGI CCWGG 5 cut(s) 589, 3456, 4521, 4609, 5062
PspN4I GGNNCC 6 cut(s) 1104, 1354, 2980, 3978, 4944, 5211
PspOMI GGGCCC 1 cut(s) 1102
PspPI GGNCC 8 cut(s) 881, 1102, 1103, 2254, 3246, 3367, 3460, 3817
PstI CTGCAG 3 cut(s) 1996, 2146, 4234
PstNI CAGNNNCTG 1 cut(s) 2887
PsuI RGATCY 7 cut(s) 672, 1375, 1895, 3121, 3142, 3401, 3646
PvuII CAGCTG 2 cut(s) 1667, 2036
RsaI GTAC 9 cut(s) 728, 1368, 2737, 3076, 3278, 4179, 5334, 5347, 5363
RsaNI GTAC 9 cut(s) 727, 1367, 2736, 3075, 3277, 4178, 5333, 5346, 5362
RseI CAYNNNNRTG 7 cut(s) 1259, 2418, 2440, 2654, 3316, 4245, 4991
SacI GAGCTC 1 cut(s) 714
SapI GCTCTTC 1 cut(s) 4404
Sau96I GGNCC 8 cut(s) 881, 1102, 1103, 2254, 3246, 3367, 3460, 3817
ScaI AGTACT 2 cut(s) 1368, 4179
ScrFI CCNGG 6 cut(s) 591, 2983, 3458, 4523, 4611, 5064
SduI GDGCHC 3 cut(s) 714, 1106, 1357
SfcI CTRYAG 5 cut(s) 1800, 1992, 2142, 3714, 4230
Sfr274I CTCGAG 1 cut(s) 320
SfuI TTCGAA 2 cut(s) 3547, 3703
SinI GGWCC 5 cut(s) 881, 2254, 3246, 3367, 3817
SlaI CTCGAG 1 cut(s) 320
SmiMI CAYNNNNRTG 7 cut(s) 1259, 2418, 2440, 2654, 3316, 4245, 4991
SpeI ACTAGT 1 cut(s) 4054
SphI GCATGC 4 cut(s) 1865, 3793, 3848, 4743
SseBI AGGCCT 2 cut(s) 1548, 4304
SspI AATATT 3 cut(s) 1298, 2452, 2469
SstI GAGCTC 1 cut(s) 714
StuI AGGCCT 2 cut(s) 1548, 4304
StyD4I CCNGG 6 cut(s) 589, 2981, 3456, 4521, 4609, 5062
StyI CCWWGG 9 cut(s) 693, 1866, 2592, 2649, 3257, 3522, 3820, 4299, 5072
TaiI ACGT 6 cut(s) 528, 1709, 1927, 2002, 3837, 4432
TaqII GACCGA 2 cut(s) 4266, 5301
TatI WGTACW 5 cut(s) 1366, 2735, 3074, 4177, 5332
TauI GCSGC 3 cut(s) 707, 962, 2513
TspGWI ACGGA 5 cut(s) 480, 2011, 2127, 3506, 4830
Van91I CCANNNNNTGG 1 cut(s) 3643
Vha464I CTTAAG 1 cut(s) 2363
VpaK11BI GGWCC 5 cut(s) 881, 2254, 3246, 3367, 3817
XagI CCTNNNNNAGG 1 cut(s) 2074
XbaI TCTAGA 2 cut(s) 890, 2156
XceI RCATGY 8 cut(s) 1624, 1832, 1865, 3793, 3848, 4173, 4743, 5042
XhoI CTCGAG 1 cut(s) 320
XmiI GTMKAC 1 cut(s) 1400
XmnI GAANNNNTTC 2 cut(s) 651, 3027
ZrmI AGTACT 2 cut(s) 1368, 4179
Zsp2I ATGCAT 6 cut(s) 1279, 1882, 2932, 4171, 5040, 5231
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.