FvH4_3g31600

Kiwellin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0012598
Physical Location & Seq
Reverse (-)
25882908 .. 25885794
2887 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g31600.t1

Sequence Viewer

Length: 651 bp
ATGCAACTTGCACGCAGACGGAGGACTCAACTGCTCATGTCTGATACTTTGTGCATACCATCTCTCTTCTCACACCCTGCACTAGTTAACAATAATATTGCTCTAATCTTTTTTCAAATGGCAAGTTTTAAGTTGTTGGTTTCTCTATCCCTCGTCTTGGCTATCACCTCCCTTCCACTCCCATCAAGTGCGCAAACTTGTAATCCCTCCGGAACTCTGGTTTGCAAGGGAAAGTCGTACCCTAAATATACATGCTCGCCGCCTGCTGTAACAGCAAAAACCGAAGCCTTGCTCACACTCAACGATTTCAGTGAAGGCGGTGACGGGGGAGGACCGTCATATTGTGACGGAAAATACCATTCAAACAGTGAGAAGGTTGTGGCACTTTCCATCGGGTGGTTTGATAATAAATCGAGATGTGGGAAGATGATCAGAATTACGGCAAGAAATGGAAAGACTACGACTGCAAAAGTTGTGGACGAATGCGATTCGCATGCTGGGTGTGATAAGGAGCATGCAGGTCAGCTGCCGTGTAGGAACAATATCGTAGATGGGTCTGCAGCTGTGTGGAAAGCTTTGGGACTAAATCAAGATGAAGGTGTTCTTGACTTGGGCCATGGCATAGTTAGCTACTTCTACTCTCATATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

217

Amino Acids

23.23

Weight (kDa)

8.56

Isoelectric Point (pI)

45.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KWL1 PF24300 62 - 201 1.2e-53 Kiwellin-1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 192
Acc36I ACCTGC 1 cut(s) 509
AccB7I CCANNNNNTGG 1 cut(s) 396
AccIII TCCGGA 1 cut(s) 209
AciI CCGC 2 cut(s) 260, 318
AfaI GTAC 1 cut(s) 239
AfiI CCNNNNNNNGG 2 cut(s) 157, 396
AgsI TTSAA 2 cut(s) 116, 363
AhlI ACTAGT 1 cut(s) 82
AluBI AGCT 4 cut(s) 526, 563, 575, 630
AluI AGCT 4 cut(s) 526, 563, 575, 630
Aor13HI TCCGGA 1 cut(s) 209
AoxI GGCC 1 cut(s) 613
ApeKI GCWGC 2 cut(s) 526, 560
Asp700I GAANNNNTTC 1 cut(s) 600
AspLEI GCGC 1 cut(s) 193
AspS9I GGNCC 2 cut(s) 332, 613
AsuHPI GGTGA 2 cut(s) 157, 332
AvaII GGWCC 1 cut(s) 332
BbvI GCAGC 2 cut(s) 513, 572
BccI CCATC 4 cut(s) 67, 190, 398, 545
BceAI ACGGC 2 cut(s) 456, 514
BcgI CGANNNNNNTGC 2 cut(s) 476, 510
BclI TGATCA 1 cut(s) 429
BcuI ACTAGT 1 cut(s) 82
BfaI CTAG 1 cut(s) 83
BfmI CTRYAG 1 cut(s) 558
BfuAI ACCTGC 1 cut(s) 509
BisI GCNGC 3 cut(s) 260, 527, 561
BlsI GCNGC 3 cut(s) 261, 528, 562
Bme18I GGWCC 1 cut(s) 332
BmgT120I GGNCC 2 cut(s) 332, 613
BsaJI CCNNGG 1 cut(s) 616
BsaWI WCCGGW 1 cut(s) 209
Bsc4I CCNNNNNNNGG 2 cut(s) 157, 396
BseAI TCCGGA 1 cut(s) 209
BseDI CCNNGG 1 cut(s) 616
BseLI CCNNNNNNNGG 2 cut(s) 157, 396
BseXI GCAGC 2 cut(s) 513, 572
BseYI CCCAGC 1 cut(s) 497
BsgI GTGCAG 1 cut(s) 63
BshFI GGCC 1 cut(s) 615
BsiSI CCGG 1 cut(s) 210
BslFI GGGAC 1 cut(s) 594
BslI CCNNNNNNNGG 2 cut(s) 157, 396
BsmFI GGGAC 1 cut(s) 594
BsmI GAATGC 1 cut(s) 488
BsnI GGCC 1 cut(s) 615
Bsp13I TCCGGA 1 cut(s) 209
Bsp143I GATC 1 cut(s) 429
Bsp19I CCATGG 1 cut(s) 616
BspACI CCGC 2 cut(s) 260, 318
BspANI GGCC 1 cut(s) 615
BspEI TCCGGA 1 cut(s) 209
BspMAI CTGCAG 1 cut(s) 562
BspMI ACCTGC 1 cut(s) 509
BssECI CCNNGG 1 cut(s) 616
BssMI GATC 1 cut(s) 429
BssT1I CCWWGG 1 cut(s) 616
Bst4CI ACNGT 2 cut(s) 336, 368
Bst6I CTCTTC 1 cut(s) 71
BstC8I GCNNGC 5 cut(s) 13, 257, 264, 495, 516
BstDSI CCRYGG 1 cut(s) 616
BstHHI GCGC 1 cut(s) 193
BstKTI GATC 1 cut(s) 432
BstMBI GATC 1 cut(s) 429
BstMWI GCNNNNNNNGC 2 cut(s) 272, 627
BstNSI RCATGY 3 cut(s) 255, 497, 518
BstSFI CTRYAG 1 cut(s) 558
BstV1I GCAGC 2 cut(s) 513, 572
BsuRI GGCC 1 cut(s) 615
BtgI CCRYGG 1 cut(s) 616
BtsIMutI CAGTG 2 cut(s) 316, 373
BveI ACCTGC 1 cut(s) 509
Cac8I GCNNGC 5 cut(s) 13, 257, 264, 495, 516
CfoI GCGC 1 cut(s) 193
Cfr13I GGNCC 2 cut(s) 332, 613
Csp6I GTAC 1 cut(s) 238
CspCI CAANNNNNGTGG 2 cut(s) 456, 491
CviAII CATG 5 cut(s) 37, 252, 494, 515, 617
CviJI RGCY 7 cut(s) 161, 287, 526, 563, 575, 615, 630
CviKI_1 RGCY 7 cut(s) 161, 287, 526, 563, 575, 615, 630
CviQI GTAC 1 cut(s) 238
DpnI GATC 1 cut(s) 431
DpnII GATC 1 cut(s) 429
Eam1104I CTCTTC 1 cut(s) 71
EarI CTCTTC 1 cut(s) 71
Eco130I CCWWGG 1 cut(s) 616
Eco47I GGWCC 1 cut(s) 332
EcoT14I CCWWGG 1 cut(s) 616
ErhI CCWWGG 1 cut(s) 616
FaeI CATG 5 cut(s) 40, 255, 497, 518, 620
FalI AAGNNNNNCTT 2 cut(s) 588, 620
FaqI GGGAC 1 cut(s) 594
FatI CATG 5 cut(s) 36, 251, 493, 514, 616
FbaI TGATCA 1 cut(s) 429
Fnu4HI GCNGC 3 cut(s) 260, 527, 561
Fsp4HI GCNGC 3 cut(s) 260, 527, 561
FspBI CTAG 1 cut(s) 83
FspI TGCGCA 1 cut(s) 192
GlaI GCGC 1 cut(s) 192
GluI GCNGC 3 cut(s) 260, 527, 561
GsaI CCCAGC 1 cut(s) 501
HaeIII GGCC 1 cut(s) 615
HapII CCGG 1 cut(s) 210
HhaI GCGC 1 cut(s) 193
Hin1II CATG 5 cut(s) 40, 255, 497, 518, 620
Hin6I GCGC 1 cut(s) 191
HinP1I GCGC 1 cut(s) 191
HincII GTYRAC 1 cut(s) 88
HindII GTYRAC 1 cut(s) 88
HindIII AAGCTT 1 cut(s) 573
HinfI GANTC 2 cut(s) 25, 488
HpaI GTTAAC 1 cut(s) 88
HpaII CCGG 1 cut(s) 210
HphI GGTGA 2 cut(s) 157, 332
Hpy166II GTNNAC 2 cut(s) 88, 478
Hpy188I TCNGA 2 cut(s) 43, 434
Hpy188III TCNNGA 4 cut(s) 210, 414, 590, 605
Hpy8I GTNNAC 2 cut(s) 88, 478
HpyAV CCTTC 4 cut(s) 182, 308, 367, 590
HpyCH4III ACNGT 2 cut(s) 336, 368
HpyCH4V TGCA 8 cut(s) 4, 11, 54, 80, 225, 467, 518, 560
HpyF10VI GCNNNNNNNGC 2 cut(s) 272, 627
Hsp92II CATG 5 cut(s) 40, 255, 497, 518, 620
HspAI GCGC 1 cut(s) 191
Kpn2I TCCGGA 1 cut(s) 209
Ksp22I TGATCA 1 cut(s) 429
KspAI GTTAAC 1 cut(s) 88
Kzo9I GATC 1 cut(s) 429
LmnI GCTCC 1 cut(s) 511
LpnPI CCDG 6 cut(s) 90, 203, 223, 276, 483, 504
Lsp1109I GCAGC 2 cut(s) 513, 572
MaeI CTAG 1 cut(s) 83
MaeIII GTNAC 3 cut(s) 268, 320, 344
MalI GATC 1 cut(s) 431
MboI GATC 1 cut(s) 429
MboII GAAGA 2 cut(s) 58, 436
MluCI AATT 1 cut(s) 435
MlyI GAGTC 1 cut(s) 19
MnlI CCTC 5 cut(s) 15, 161, 178, 217, 323
MroI TCCGGA 1 cut(s) 209
MroXI GAANNNNTTC 1 cut(s) 600
MseI TTAA 2 cut(s) 87, 129
MspA1I CMGCKG 2 cut(s) 526, 563
MspI CCGG 1 cut(s) 210
Mva1269I GAATGC 1 cut(s) 488
MwoI GCNNNNNNNGC 2 cut(s) 272, 627
NcoI CCATGG 1 cut(s) 616
NdeII GATC 1 cut(s) 429
NlaIII CATG 5 cut(s) 40, 255, 497, 518, 620
NmuCI GTSAC 2 cut(s) 320, 344
NsbI TGCGCA 1 cut(s) 192
NspI RCATGY 3 cut(s) 255, 497, 518
PaeI GCATGC 2 cut(s) 497, 518
PctI GAATGC 1 cut(s) 488
PdmI GAANNNNTTC 1 cut(s) 600
PfeI GAWTC 1 cut(s) 488
PflMI CCANNNNNTGG 1 cut(s) 396
PkrI GCNGC 3 cut(s) 261, 528, 562
PleI GAGTC 1 cut(s) 19
PpsI GAGTC 1 cut(s) 19
PspFI CCCAGC 1 cut(s) 497
PspPI GGNCC 2 cut(s) 332, 613
PstI CTGCAG 1 cut(s) 562
PvuII CAGCTG 2 cut(s) 526, 563
RsaI GTAC 1 cut(s) 239
RsaNI GTAC 1 cut(s) 238
SaqAI TTAA 2 cut(s) 87, 129
SatI GCNGC 3 cut(s) 260, 527, 561
Sau3AI GATC 1 cut(s) 429
Sau96I GGNCC 2 cut(s) 332, 613
SchI GAGTC 1 cut(s) 19
SetI ASST 8 cut(s) 170, 378, 523, 528, 565, 577, 601, 632
SfcI CTRYAG 1 cut(s) 558
SinI GGWCC 1 cut(s) 332
SpeI ACTAGT 1 cut(s) 82
SphI GCATGC 2 cut(s) 497, 518
Sse9I AATT 1 cut(s) 435
SsiI CCGC 2 cut(s) 260, 318
SspI AATATT 1 cut(s) 97
SspMI CTAG 1 cut(s) 83
StyI CCWWGG 1 cut(s) 616
TaaI ACNGT 2 cut(s) 336, 368
TaqI TCGA 1 cut(s) 413
TasI AATT 1 cut(s) 435
TauI GCSGC 1 cut(s) 262
TfiI GAWTC 1 cut(s) 488
Tru1I TTAA 2 cut(s) 87, 129
Tru9I TTAA 2 cut(s) 87, 129
TscAI CASTG 2 cut(s) 316, 373
TseFI GTSAC 2 cut(s) 320, 344
TseI GCWGC 2 cut(s) 526, 560
Tsp45I GTSAC 2 cut(s) 320, 344
TspDTI ATGAA 1 cut(s) 609
TspGWI ACGGA 2 cut(s) 34, 363
TspRI CASTG 2 cut(s) 316, 373
Van91I CCANNNNNTGG 1 cut(s) 396
VpaK11BI GGWCC 1 cut(s) 332
XceI RCATGY 3 cut(s) 255, 497, 518
XmnI GAANNNNTTC 1 cut(s) 600
XspI CTAG 1 cut(s) 83
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.