MD11G1140700.v1.1

Kiwellin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0012598
Physical Location & Seq
Forward (+)
13132115 .. 13132768
654 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1140700.v1.1.491

Sequence Viewer

Length: 654 bp
ATGGAAAGTAATTTAGGGTTTTTGGTTCCCCTTACCCTCATCGTCCTTAGTATCGTCTCCCTCCCTTTCCCGTCCACCGCCATCTCCTCCTGCAATGACGCATGCAAAACCCTCGACGACTGCGCTGGCCAGCTCATCTGCATCAACGGAAAGTGTAACGACGACCCCGATATTGGGTCGACAGAATGCAGACACGGCTCTTCACCACCTCCTACGACGGACAACTGTAAGTCGTCAGGAACTCTAGTTTGCAACGGCAAATCATACCCTACATACACATGCTCTCCAGCTGTGACGTCATCCACAAAAGCCAGCCTTACTCTCAACGATTTCAGCCAAGGCGGGGATGGCGGTGCCCCGTCATATTGTGACGGAAAATACCATAACAACAGCGAGCGTGTTGTGGCACTTTCCACTGGGTGGTTTAATAATAAGTCGAGGTGTTTTAATCTGATAAGAATTACGGCAAGCAATGGGAAGAGTGTGACGGCAAAAGTGGTTGACGAATGTGATTCGCGTGCTGGATGCGATGAGGAGCATGGAGGTCAACCGCCGTGTCGGAACAACATTGTTGATGGATCTTCGGCTGTGTGGAACGCTTTGGGACTGAACAAAGATTTGGGCGTTGTGCCTGTTACTTGGTCCATGGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

218

Amino Acids

22.74

Weight (kDa)

5.1

Isoelectric Point (pI)

47.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KWL1 PF24300 63 - 215 7.6e-57 Kiwellin-1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 299
AccB1I GGYRCC 1 cut(s) 353
AccB7I CCANNNNNTGG 1 cut(s) 420
AccI GTMKAC 1 cut(s) 179
AccII CGCG 1 cut(s) 517
AciI CCGC 4 cut(s) 78, 342, 351, 551
AclWI GGATC 1 cut(s) 586
AcoI YGGCCR 1 cut(s) 127
AcyI GRCGYC 1 cut(s) 296
AdeI CACNNNGTG 1 cut(s) 420
AfiI CCNNNNNNNGG 4 cut(s) 173, 174, 343, 420
AjuI GAANNNNNNNTTGG 2 cut(s) 602, 634
AluBI AGCT 2 cut(s) 133, 290
AluI AGCT 2 cut(s) 133, 290
Alw26I GTCTC 1 cut(s) 61
AlwI GGATC 1 cut(s) 586
AoxI GGCC 2 cut(s) 127, 648
AspLEI GCGC 1 cut(s) 125
AspS9I GGNCC 1 cut(s) 642
AsuHPI GGTGA 1 cut(s) 195
AvaII GGWCC 1 cut(s) 642
BaeGI GKGCMC 1 cut(s) 358
BalI TGGCCA 1 cut(s) 129
BanI GGYRCC 1 cut(s) 353
BccI CCATC 3 cut(s) 89, 341, 569
BceAI ACGGC 5 cut(s) 211, 271, 480, 504, 538
BcgI CGANNNNNNTGC 2 cut(s) 94, 128
BcoDI GTCTC 1 cut(s) 61
BfaI CTAG 2 cut(s) 245, 652
Bme18I GGWCC 1 cut(s) 642
BmgT120I GGNCC 1 cut(s) 642
BmiI GGNNCC 2 cut(s) 27, 355
BmrI ACTGGG 1 cut(s) 426
BmsI GCATC 2 cut(s) 150, 515
BmuI ACTGGG 1 cut(s) 426
BpmI CTGGAG 1 cut(s) 270
BsaHI GRCGYC 1 cut(s) 296
BsaJI CCNNGG 2 cut(s) 337, 645
BsaXI ACNNNNNCTCC 2 cut(s) 268, 298
Bsc4I CCNNNNNNNGG 4 cut(s) 173, 174, 343, 420
Bse1I ACTGG 1 cut(s) 421
Bse3DI GCAATG 2 cut(s) 100, 478
BseDI CCNNGG 2 cut(s) 337, 645
BseGI GGATG 3 cut(s) 299, 352, 530
BseLI CCNNNNNNNGG 4 cut(s) 173, 174, 343, 420
BseMI GCAATG 2 cut(s) 100, 478
BseNI ACTGG 1 cut(s) 421
BseRI GAGGAG 2 cut(s) 76, 548
BseSI GKGCMC 1 cut(s) 358
Bsh1236I CGCG 1 cut(s) 517
BshFI GGCC 2 cut(s) 129, 650
BshNI GGYRCC 1 cut(s) 353
BslFI GGGAC 1 cut(s) 618
BslI CCNNNNNNNGG 4 cut(s) 173, 174, 343, 420
BsmAI GTCTC 1 cut(s) 61
BsmBI CGTCTC 1 cut(s) 61
BsmFI GGGAC 1 cut(s) 618
BsmI GAATGC 1 cut(s) 191
BsnI GGCC 2 cut(s) 129, 650
Bsp1286I GDGCHC 1 cut(s) 358
Bsp143I GATC 1 cut(s) 578
Bsp19I CCATGG 1 cut(s) 645
BspACI CCGC 4 cut(s) 78, 342, 351, 551
BspANI GGCC 2 cut(s) 129, 650
BspFNI CGCG 1 cut(s) 517
BspLI GGNNCC 2 cut(s) 27, 355
BspPI GGATC 1 cut(s) 586
BspQI GCTCTTC 1 cut(s) 205
BspT107I GGYRCC 1 cut(s) 353
BsrDI GCAATG 2 cut(s) 100, 478
BsrI ACTGG 1 cut(s) 421
BssECI CCNNGG 2 cut(s) 337, 645
BssMI GATC 1 cut(s) 578
BssNI GRCGYC 1 cut(s) 296
BssT1I CCWWGG 2 cut(s) 337, 645
Bst4CI ACNGT 1 cut(s) 227
Bst6I CTCTTC 2 cut(s) 205, 473
BstACI GRCGYC 1 cut(s) 296
BstC8I GCNNGC 7 cut(s) 103, 127, 131, 313, 395, 469, 519
BstDEI CTNAG 1 cut(s) 47
BstDSI CCRYGG 1 cut(s) 645
BstF5I GGATG 3 cut(s) 299, 352, 530
BstFNI CGCG 1 cut(s) 517
BstHHI GCGC 1 cut(s) 125
BstKTI GATC 1 cut(s) 581
BstMAI GTCTC 1 cut(s) 61
BstMBI GATC 1 cut(s) 578
BstMWI GCNNNNNNNGC 2 cut(s) 195, 348
BstNSI RCATGY 2 cut(s) 105, 282
BstSLI GKGCMC 1 cut(s) 358
BstUI CGCG 1 cut(s) 517
BstX2I RGATCY 1 cut(s) 578
BstYI RGATCY 1 cut(s) 578
BsuRI GGCC 2 cut(s) 129, 650
BtgI CCRYGG 1 cut(s) 645
BtgZI GCGATG 1 cut(s) 543
BtsCI GGATG 3 cut(s) 299, 352, 530
BtsIMutI CAGTG 1 cut(s) 414
Cac8I GCNNGC 7 cut(s) 103, 127, 131, 313, 395, 469, 519
CfoI GCGC 1 cut(s) 125
Cfr13I GGNCC 1 cut(s) 642
CseI GACGC 1 cut(s) 107
CviAII CATG 4 cut(s) 102, 279, 539, 646
CviJI RGCY 9 cut(s) 129, 133, 198, 290, 311, 315, 336, 587, 650
CviKI_1 RGCY 9 cut(s) 129, 133, 198, 290, 311, 315, 336, 587, 650
DdeI CTNAG 1 cut(s) 47
DpnI GATC 1 cut(s) 580
DpnII GATC 1 cut(s) 578
DraIII CACNNNGTG 1 cut(s) 420
EaeI YGGCCR 1 cut(s) 127
Eam1104I CTCTTC 2 cut(s) 205, 473
EarI CTCTTC 2 cut(s) 205, 473
Eco130I CCWWGG 2 cut(s) 337, 645
Eco47I GGWCC 1 cut(s) 642
EcoT14I CCWWGG 2 cut(s) 337, 645
ErhI CCWWGG 2 cut(s) 337, 645
Esp3I CGTCTC 1 cut(s) 61
FaeI CATG 4 cut(s) 105, 282, 542, 649
FaiI YATR 8 cut(s) 103, 265, 274, 280, 364, 384, 540, 647
FalI AAGNNNNNCTT 2 cut(s) 300, 332
FaqI GGGAC 1 cut(s) 618
FatI CATG 4 cut(s) 101, 278, 538, 645
FauI CCCGC 1 cut(s) 335
FblI GTMKAC 1 cut(s) 179
FokI GGATG 3 cut(s) 286, 359, 537
FspBI CTAG 2 cut(s) 245, 652
GlaI GCGC 1 cut(s) 124
GsuI CTGGAG 1 cut(s) 270
HaeIII GGCC 2 cut(s) 129, 650
HgaI GACGC 1 cut(s) 107
HhaI GCGC 1 cut(s) 125
Hin1I GRCGYC 1 cut(s) 296
Hin1II CATG 4 cut(s) 105, 282, 542, 649
Hin6I GCGC 1 cut(s) 123
HinP1I GCGC 1 cut(s) 123
HincII GTYRAC 3 cut(s) 180, 502, 548
HindII GTYRAC 3 cut(s) 180, 502, 548
HinfI GANTC 1 cut(s) 512
HphI GGTGA 1 cut(s) 195
Hpy166II GTNNAC 4 cut(s) 75, 180, 502, 548
Hpy188I TCNGA 2 cut(s) 453, 561
Hpy188III TCNNGA 1 cut(s) 237
Hpy8I GTNNAC 4 cut(s) 75, 180, 502, 548
Hpy99I CGWCG 3 cut(s) 119, 164, 220
HpyCH4III ACNGT 1 cut(s) 227
HpyCH4IV ACGT 1 cut(s) 296
HpyCH4V TGCA 5 cut(s) 93, 105, 141, 189, 252
HpyF10VI GCNNNNNNNGC 2 cut(s) 195, 348
HpyF3I CTNAG 1 cut(s) 47
HpySE526I ACGT 1 cut(s) 296
Hsp92I GRCGYC 1 cut(s) 296
Hsp92II CATG 4 cut(s) 105, 282, 542, 649
HspAI GCGC 1 cut(s) 123
Kzo9I GATC 1 cut(s) 578
LguI GCTCTTC 1 cut(s) 205
LmnI GCTCC 1 cut(s) 535
LpnPI CCDG 9 cut(s) 103, 111, 143, 222, 300, 325, 402, 507, 645
LweI GCATC 2 cut(s) 150, 515
MaeI CTAG 2 cut(s) 245, 652
MaeII ACGT 1 cut(s) 296
MaeIII GTNAC 5 cut(s) 155, 292, 368, 484, 634
MalI GATC 1 cut(s) 580
MboI GATC 1 cut(s) 578
MboII GAAGA 3 cut(s) 192, 490, 573
MflI RGATCY 1 cut(s) 578
MhlI GDGCHC 1 cut(s) 358
MlsI TGGCCA 1 cut(s) 129
MluCI AATT 2 cut(s) 10, 459
MluNI TGGCCA 1 cut(s) 129
MmeI TCCRAC 1 cut(s) 539
MnlI CCTC 8 cut(s) 47, 71, 97, 122, 219, 432, 526, 536
Mox20I TGGCCA 1 cut(s) 129
MscI TGGCCA 1 cut(s) 129
MseI TTAA 2 cut(s) 426, 447
MslI CAYNNNNRTG 1 cut(s) 277
Msp20I TGGCCA 1 cut(s) 129
MspA1I CMGCKG 1 cut(s) 290
Mva1269I GAATGC 1 cut(s) 191
MvnI CGCG 1 cut(s) 517
MwoI GCNNNNNNNGC 2 cut(s) 195, 348
NcoI CCATGG 1 cut(s) 645
NdeII GATC 1 cut(s) 578
NlaIII CATG 4 cut(s) 105, 282, 542, 649
NlaIV GGNNCC 2 cut(s) 27, 355
NmuCI GTSAC 3 cut(s) 292, 368, 484
NspI RCATGY 2 cut(s) 105, 282
PaeI GCATGC 1 cut(s) 105
PciSI GCTCTTC 1 cut(s) 205
PcsI WCGNNNNNNNCGW 1 cut(s) 165
PctI GAATGC 1 cut(s) 191
PfeI GAWTC 1 cut(s) 512
PflMI CCANNNNNTGG 1 cut(s) 420
PspN4I GGNNCC 2 cut(s) 27, 355
PspPI GGNCC 1 cut(s) 642
PsuI RGATCY 1 cut(s) 578
PvuII CAGCTG 1 cut(s) 290
RseI CAYNNNNRTG 1 cut(s) 277
SalI GTCGAC 1 cut(s) 178
SapI GCTCTTC 1 cut(s) 205
SaqAI TTAA 2 cut(s) 426, 447
Sau3AI GATC 1 cut(s) 578
Sau96I GGNCC 1 cut(s) 642
SduI GDGCHC 1 cut(s) 358
SetI ASST 6 cut(s) 135, 211, 292, 299, 443, 547
SfaNI GCATC 2 cut(s) 150, 515
SinI GGWCC 1 cut(s) 642
SmiMI CAYNNNNRTG 1 cut(s) 277
SphI GCATGC 1 cut(s) 105
Sse9I AATT 2 cut(s) 10, 459
SsiI CCGC 4 cut(s) 78, 342, 351, 551
SspMI CTAG 2 cut(s) 245, 652
StyI CCWWGG 2 cut(s) 337, 645
TaaI ACNGT 1 cut(s) 227
TaiI ACGT 1 cut(s) 299
TaqI TCGA 3 cut(s) 114, 179, 437
TasI AATT 2 cut(s) 10, 459
TfiI GAWTC 1 cut(s) 512
Tru1I TTAA 2 cut(s) 426, 447
Tru9I TTAA 2 cut(s) 426, 447
TscAI CASTG 1 cut(s) 421
TseFI GTSAC 3 cut(s) 292, 368, 484
Tsp45I GTSAC 3 cut(s) 292, 368, 484
TspGWI ACGGA 3 cut(s) 162, 233, 387
TspRI CASTG 1 cut(s) 421
Van91I CCANNNNNTGG 1 cut(s) 420
VpaK11BI GGWCC 1 cut(s) 642
XceI RCATGY 2 cut(s) 105, 282
XcmI CCANNNNNNNNNTGG 1 cut(s) 344
XmiI GTMKAC 1 cut(s) 179
XspI CTAG 2 cut(s) 245, 652
ZraI GACGTC 1 cut(s) 297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.