RLG00000035206

Kiwellin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0012598
Physical Location & Seq
Reverse (-)
62848596 .. 62849466
871 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035206

Sequence Viewer

Length: 687 bp
ATGGCAAGTTCTTTAGTATGTTTGGCATCTCTTTCCCTCGTCTTCATTTTCATCTCCATCCCTCTCCCGTACTCCACAGCTATCTCCTCCTGCAATGGCGCATGCAAAACCTTGAACGACTGTGCTGGCCAACTCATCTGTATCAACCAAAAATGCAACGATGACCCCGATGTTGGGACTCACATATGCGGCAGGGGTGGGGGAGGAGGCTCATCACCTGCTCCTAGCCCCAACAATTGCAGACCCTTTGGAACACTGGTTTGCAACGGCAAGTCGTACCCTAAATACTCGTGCTCTCCCCCAGTAACATCATCCACCGAAGCCTTACTTACACTCAACGATTTCAGCGAAGGCGGTGATGGGGGCGGGCCATCAGAATGTGACGAACAATATCATCCAAACAGCGAGCGGGTTGTGGCACTTTCCACTGGGTGGTTTGATAATAAATCGAGGTGCGGGAAGGTAATAAGAATTAAGGCAAGTAATGGGAAGACTACGACTGCGAAAGTGGTGGATGAATGTGATTCGCGTAATGGATGTGATAAAGAGCATGCAGGGCAGCCGACGTGTGGAAACAATATCGTGGATGGATCTGCGTCTGTGTGGAACGCTTTGGGACTCGATCAGAATCTAGTACTGTTGGATTTTGTGTTTGTTATGTGTGGCGAGCTTCTGTTACTTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

229

Amino Acids

23.85

Weight (kDa)

5.15

Isoelectric Point (pI)

45.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KWL1 PF24300 82 - 209 1.8e-50 Kiwellin-1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 226
Acc36I ACCTGC 1 cut(s) 226
AccB7I CCANNNNNTGG 1 cut(s) 432
AccBSI CCGCTC 1 cut(s) 409
AccII CGCG 1 cut(s) 529
AciI CCGC 5 cut(s) 189, 354, 366, 409, 456
AclWI GGATC 1 cut(s) 598
AcoI YGGCCR 1 cut(s) 127
AdeI CACNNNGTG 1 cut(s) 432
AfaI GTAC 3 cut(s) 71, 278, 636
AfiI CCNNNNNNNGG 4 cut(s) 173, 174, 432, 569
AflIII ACRYGT 1 cut(s) 566
AgsI TTSAA 1 cut(s) 115
AjiI CACGTC 1 cut(s) 567
AluBI AGCT 2 cut(s) 80, 670
AluI AGCT 2 cut(s) 80, 670
Alw21I GWGCWC 1 cut(s) 296
AlwI GGATC 1 cut(s) 598
AoxI GGCC 2 cut(s) 127, 368
ApeKI GCWGC 1 cut(s) 559
AspLEI GCGC 1 cut(s) 101
AspS9I GGNCC 1 cut(s) 368
AsuHPI GGTGA 2 cut(s) 207, 368
BalI TGGCCA 1 cut(s) 129
BauI CACGAG 1 cut(s) 289
BbsI GAAGAC 2 cut(s) 34, 497
Bbv12I GWGCWC 1 cut(s) 296
BbvI GCAGC 1 cut(s) 571
BccI CCATC 4 cut(s) 65, 353, 379, 581
BceAI ACGGC 1 cut(s) 283
BfaI CTAG 2 cut(s) 225, 632
BfuAI ACCTGC 1 cut(s) 226
BisI GCNGC 2 cut(s) 190, 560
BlsI GCNGC 2 cut(s) 191, 561
BmcAI AGTACT 1 cut(s) 636
BmgBI CACGTC 1 cut(s) 567
BmgT120I GGNCC 1 cut(s) 368
BmrI ACTGGG 2 cut(s) 296, 438
BmsI GCATC 1 cut(s) 35
BmuI ACTGGG 2 cut(s) 296, 438
BpiI GAAGAC 2 cut(s) 34, 497
BsaBI GATNNNNATC 1 cut(s) 627
Bsc4I CCNNNNNNNGG 4 cut(s) 173, 174, 432, 569
Bse1I ACTGG 3 cut(s) 261, 302, 433
Bse3DI GCAATG 1 cut(s) 100
Bse8I GATNNNNATC 1 cut(s) 627
BseGI GGATG 6 cut(s) 57, 311, 394, 520, 542, 592
BseJI GATNNNNATC 1 cut(s) 627
BseLI CCNNNNNNNGG 4 cut(s) 173, 174, 432, 569
BseMI GCAATG 1 cut(s) 100
BseNI ACTGG 3 cut(s) 261, 302, 433
BseRI GAGGAG 2 cut(s) 76, 219
BseXI GCAGC 1 cut(s) 571
Bsh1236I CGCG 1 cut(s) 529
BshFI GGCC 2 cut(s) 129, 370
BsiHKAI GWGCWC 1 cut(s) 296
BslFI GGGAC 2 cut(s) 190, 630
BslI CCNNNNNNNGG 4 cut(s) 173, 174, 432, 569
BsmFI GGGAC 2 cut(s) 190, 630
BsnI GGCC 2 cut(s) 129, 370
Bsp1286I GDGCHC 1 cut(s) 296
Bsp143I GATC 2 cut(s) 590, 622
BspACI CCGC 5 cut(s) 189, 354, 366, 409, 456
BspANI GGCC 2 cut(s) 129, 370
BspFNI CGCG 1 cut(s) 529
BspMI ACCTGC 1 cut(s) 226
BspPI GGATC 1 cut(s) 598
BsrBI CCGCTC 1 cut(s) 409
BsrDI GCAATG 1 cut(s) 100
BsrI ACTGG 3 cut(s) 261, 302, 433
BssMI GATC 2 cut(s) 590, 622
BssSI CACGAG 1 cut(s) 289
Bst2BI CACGAG 1 cut(s) 289
Bst4CI ACNGT 2 cut(s) 122, 639
BstC8I GCNNGC 6 cut(s) 103, 127, 368, 407, 552, 668
BstF5I GGATG 6 cut(s) 57, 311, 394, 520, 542, 592
BstFNI CGCG 1 cut(s) 529
BstHHI GCGC 1 cut(s) 101
BstKTI GATC 2 cut(s) 593, 625
BstMBI GATC 2 cut(s) 590, 622
BstMWI GCNNNNNNNGC 1 cut(s) 556
BstNSI RCATGY 2 cut(s) 105, 554
BstUI CGCG 1 cut(s) 529
BstV1I GCAGC 1 cut(s) 571
BstV2I GAAGAC 2 cut(s) 34, 497
BstX2I RGATCY 1 cut(s) 590
BstYI RGATCY 1 cut(s) 590
BsuRI GGCC 2 cut(s) 129, 370
BtrI CACGTC 1 cut(s) 567
BtsCI GGATG 6 cut(s) 57, 311, 394, 520, 542, 592
BtsIMutI CAGTG 2 cut(s) 254, 426
BveI ACCTGC 1 cut(s) 226
Cac8I GCNNGC 6 cut(s) 103, 127, 368, 407, 552, 668
CfoI GCGC 1 cut(s) 101
Cfr13I GGNCC 1 cut(s) 368
CseI GACGC 1 cut(s) 585
Csp6I GTAC 3 cut(s) 70, 277, 635
CviAII CATG 2 cut(s) 102, 551
CviJI RGCY 8 cut(s) 80, 129, 210, 228, 323, 370, 562, 670
CviKI_1 RGCY 8 cut(s) 80, 129, 210, 228, 323, 370, 562, 670
CviQI GTAC 3 cut(s) 70, 277, 635
DpnI GATC 2 cut(s) 592, 624
DpnII GATC 2 cut(s) 590, 622
DraIII CACNNNGTG 1 cut(s) 432
EaeI YGGCCR 1 cut(s) 127
FaeI CATG 2 cut(s) 105, 554
FaiI YATR 6 cut(s) 19, 103, 185, 187, 552, 659
FalI AAGNNNNNCTT 2 cut(s) 312, 344
FaqI GGGAC 2 cut(s) 190, 630
FatI CATG 2 cut(s) 101, 550
FauI CCCGC 3 cut(s) 359, 402, 449
FauNDI CATATG 1 cut(s) 185
Fnu4HI GCNGC 2 cut(s) 190, 560
FokI GGATG 6 cut(s) 44, 298, 381, 527, 549, 599
Fsp4HI GCNGC 2 cut(s) 190, 560
FspBI CTAG 2 cut(s) 225, 632
GlaI GCGC 1 cut(s) 100
GluI GCNGC 2 cut(s) 190, 560
HaeIII GGCC 2 cut(s) 129, 370
HgaI GACGC 1 cut(s) 585
HhaI GCGC 1 cut(s) 101
Hin1II CATG 2 cut(s) 105, 554
Hin6I GCGC 1 cut(s) 99
HinP1I GCGC 1 cut(s) 99
HinfI GANTC 4 cut(s) 178, 524, 618, 628
HphI GGTGA 2 cut(s) 207, 368
Hpy188I TCNGA 2 cut(s) 376, 627
Hpy99I CGWCG 1 cut(s) 568
HpyAV CCTTC 2 cut(s) 344, 454
HpyCH4III ACNGT 2 cut(s) 122, 639
HpyCH4IV ACGT 1 cut(s) 566
HpyCH4V TGCA 6 cut(s) 93, 105, 156, 240, 264, 554
HpyF10VI GCNNNNNNNGC 1 cut(s) 556
HpySE526I ACGT 1 cut(s) 566
Hsp92II CATG 2 cut(s) 105, 554
HspAI GCGC 1 cut(s) 99
Kzo9I GATC 2 cut(s) 590, 622
LmnI GCTCC 1 cut(s) 226
LpnPI CCDG 8 cut(s) 103, 111, 178, 231, 242, 315, 414, 540
Lsp1109I GCAGC 1 cut(s) 571
LweI GCATC 1 cut(s) 35
MaeI CTAG 2 cut(s) 225, 632
MaeII ACGT 1 cut(s) 566
MaeIII GTNAC 3 cut(s) 304, 380, 675
MalI GATC 2 cut(s) 592, 624
MbiI CCGCTC 1 cut(s) 409
MboI GATC 2 cut(s) 590, 622
MboII GAAGA 2 cut(s) 34, 502
MfeI CAATTG 1 cut(s) 235
MflI RGATCY 1 cut(s) 590
MhlI GDGCHC 1 cut(s) 296
MlsI TGGCCA 1 cut(s) 129
MluCI AATT 2 cut(s) 235, 471
MluNI TGGCCA 1 cut(s) 129
MlyI GAGTC 2 cut(s) 172, 612
MmeI TCCRAC 1 cut(s) 621
MnlI CCTC 6 cut(s) 47, 72, 97, 197, 200, 444
Mox20I TGGCCA 1 cut(s) 129
MscI TGGCCA 1 cut(s) 129
MseI TTAA 2 cut(s) 474, 681
MslI CAYNNNNRTG 1 cut(s) 376
Msp20I TGGCCA 1 cut(s) 129
MunI CAATTG 1 cut(s) 235
MvnI CGCG 1 cut(s) 529
MwoI GCNNNNNNNGC 1 cut(s) 556
NdeI CATATG 1 cut(s) 185
NdeII GATC 2 cut(s) 590, 622
NlaIII CATG 2 cut(s) 105, 554
NmuCI GTSAC 1 cut(s) 380
NspI RCATGY 2 cut(s) 105, 554
PaeI GCATGC 2 cut(s) 105, 554
PaqCI CACCTGC 1 cut(s) 226
PcsI WCGNNNNNNNCGW 2 cut(s) 165, 345
PfeI GAWTC 2 cut(s) 524, 628
PflMI CCANNNNNTGG 1 cut(s) 432
PkrI GCNGC 2 cut(s) 191, 561
PleI GAGTC 2 cut(s) 172, 612
PpsI GAGTC 2 cut(s) 172, 612
PspPI GGNCC 1 cut(s) 368
PsuI RGATCY 1 cut(s) 590
RsaI GTAC 3 cut(s) 71, 278, 636
RsaNI GTAC 3 cut(s) 70, 277, 635
RseI CAYNNNNRTG 1 cut(s) 376
SaqAI TTAA 2 cut(s) 474, 681
SatI GCNGC 2 cut(s) 190, 560
Sau3AI GATC 2 cut(s) 590, 622
Sau96I GGNCC 1 cut(s) 368
ScaI AGTACT 1 cut(s) 636
SchI GAGTC 2 cut(s) 172, 612
SduI GDGCHC 1 cut(s) 296
SetI ASST 7 cut(s) 82, 113, 220, 455, 465, 569, 672
SfaNI GCATC 1 cut(s) 35
SmiMI CAYNNNNRTG 1 cut(s) 376
SphI GCATGC 2 cut(s) 105, 554
Sse9I AATT 2 cut(s) 235, 471
SsiI CCGC 5 cut(s) 189, 354, 366, 409, 456
SspMI CTAG 2 cut(s) 225, 632
TaaI ACNGT 2 cut(s) 122, 639
TaiI ACGT 1 cut(s) 569
TaqI TCGA 2 cut(s) 449, 621
TasI AATT 2 cut(s) 235, 471
TatI WGTACW 1 cut(s) 634
TauI GCSGC 1 cut(s) 192
TfiI GAWTC 2 cut(s) 524, 628
Tru1I TTAA 2 cut(s) 474, 681
Tru9I TTAA 2 cut(s) 474, 681
TscAI CASTG 2 cut(s) 261, 433
TseFI GTSAC 1 cut(s) 380
TseI GCWGC 1 cut(s) 559
Tsp45I GTSAC 1 cut(s) 380
TspDTI ATGAA 3 cut(s) 34, 40, 531
TspRI CASTG 2 cut(s) 261, 433
Van91I CCANNNNNTGG 1 cut(s) 432
XceI RCATGY 2 cut(s) 105, 554
XspI CTAG 2 cut(s) 225, 632
ZrmI AGTACT 1 cut(s) 636
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.