RchiOBHm_Chr5g0058241

Kiwellin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0012598
Physical Location & Seq
Reverse (-)
62441897 .. 62444972
3076 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ33494

Sequence Viewer

Length: 594 bp
ATGACGGCTATCTCCTCCTGCAATGGCGCATGCAAAACCTTGAACGACTGTGCTGGCCAACTCATCTGTATCAACCAAAAATGCAACGATGACCCCGATGTTGGGACTCACATATGCGGCGGCGGTGGGGGAGGAGGCTCATCACCTTCTCCTAGCCCCAACAATTGCAGACCCTTTGGAACACTGGTTTGCAAGGGCAAGTCGTACCCTAAATACTCGTGCTCTCCCCCTGTAACATCGTCCACCAAAGCCTTACTTACACTCAATGATTTCAGCGAAGGCGGTGATGGGGGCGGGCCATCAGAATGTGACGAACAATATCATCCAAACAGTGAGCAGGTTGTGGCTCTTTCCACTGGGTGGTTTGATAATAAATCGAGGTGCGGGAAGTTAATAAGAATTCAGGCAAGTAATGGGAAGACTACGACTGCGAAAGTGGTGGATGAATGTGATTCGCGTAATGGATGTGATAAAGAGCATGCAGGGCAGCCGACGTGTGGAAACAATATCGTGGATGGATCTGCATCTGTGTGGAACGCTTTGGGACTCGATCAGAATCAAGGTAAGGTGTCGGTGACTTGGTCCATGGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

197

Amino Acids

20.37

Weight (kDa)

5.55

Isoelectric Point (pI)

50.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KWL1 PF24300 47 - 195 1e-54 Kiwellin-1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 328
AccB7I CCANNNNNTGG 1 cut(s) 360
AccII CGCG 1 cut(s) 457
AciI CCGC 6 cut(s) 117, 120, 123, 282, 294, 384
AclWI GGATC 1 cut(s) 526
AcoI YGGCCR 1 cut(s) 55
AcsI RAATTY 1 cut(s) 399
AdeI CACNNNGTG 1 cut(s) 360
AfaI GTAC 1 cut(s) 206
AfiI CCNNNNNNNGG 4 cut(s) 101, 102, 360, 497
AflIII ACRYGT 1 cut(s) 494
AgsI TTSAA 1 cut(s) 43
AjiI CACGTC 1 cut(s) 495
Alw21I GWGCWC 1 cut(s) 224
AlwI GGATC 1 cut(s) 526
AoxI GGCC 2 cut(s) 55, 296
ApeKI GCWGC 1 cut(s) 487
ApoI RAATTY 1 cut(s) 399
AspLEI GCGC 1 cut(s) 29
AspS9I GGNCC 2 cut(s) 296, 582
AsuHPI GGTGA 3 cut(s) 135, 296, 586
AvaII GGWCC 1 cut(s) 582
BalI TGGCCA 1 cut(s) 57
BauI CACGAG 1 cut(s) 217
BbsI GAAGAC 1 cut(s) 425
Bbv12I GWGCWC 1 cut(s) 224
BbvI GCAGC 1 cut(s) 499
BccI CCATC 3 cut(s) 281, 307, 509
BceAI ACGGC 1 cut(s) 21
BfaI CTAG 1 cut(s) 153
BfuAI ACCTGC 1 cut(s) 328
BisI GCNGC 3 cut(s) 118, 121, 488
BlsI GCNGC 3 cut(s) 119, 122, 489
Bme18I GGWCC 1 cut(s) 582
BmgBI CACGTC 1 cut(s) 495
BmgT120I GGNCC 2 cut(s) 296, 582
BmrI ACTGGG 1 cut(s) 366
BmsI GCATC 1 cut(s) 533
BmuI ACTGGG 1 cut(s) 366
BpiI GAAGAC 1 cut(s) 425
BsaBI GATNNNNATC 2 cut(s) 523, 555
BsaJI CCNNGG 1 cut(s) 585
Bsc4I CCNNNNNNNGG 4 cut(s) 101, 102, 360, 497
Bse1I ACTGG 2 cut(s) 189, 361
Bse3DI GCAATG 1 cut(s) 28
Bse8I GATNNNNATC 2 cut(s) 523, 555
BseDI CCNNGG 1 cut(s) 585
BseGI GGATG 4 cut(s) 322, 448, 470, 520
BseJI GATNNNNATC 2 cut(s) 523, 555
BseLI CCNNNNNNNGG 4 cut(s) 101, 102, 360, 497
BseMI GCAATG 1 cut(s) 28
BseNI ACTGG 2 cut(s) 189, 361
BseRI GAGGAG 2 cut(s) 4, 147
BseXI GCAGC 1 cut(s) 499
Bsh1236I CGCG 1 cut(s) 457
BshFI GGCC 2 cut(s) 57, 298
BsiHKAI GWGCWC 1 cut(s) 224
BslFI GGGAC 2 cut(s) 118, 558
BslI CCNNNNNNNGG 4 cut(s) 101, 102, 360, 497
BsmFI GGGAC 2 cut(s) 118, 558
BsnI GGCC 2 cut(s) 57, 298
Bsp1286I GDGCHC 1 cut(s) 224
Bsp143I GATC 2 cut(s) 518, 550
Bsp19I CCATGG 1 cut(s) 585
BspACI CCGC 6 cut(s) 117, 120, 123, 282, 294, 384
BspANI GGCC 2 cut(s) 57, 298
BspFNI CGCG 1 cut(s) 457
BspMI ACCTGC 1 cut(s) 328
BspPI GGATC 1 cut(s) 526
BsrDI GCAATG 1 cut(s) 28
BsrI ACTGG 2 cut(s) 189, 361
BssECI CCNNGG 1 cut(s) 585
BssMI GATC 2 cut(s) 518, 550
BssSI CACGAG 1 cut(s) 217
BssT1I CCWWGG 1 cut(s) 585
Bst2BI CACGAG 1 cut(s) 217
Bst4CI ACNGT 2 cut(s) 50, 332
BstC8I GCNNGC 4 cut(s) 31, 55, 296, 480
BstDSI CCRYGG 1 cut(s) 585
BstF5I GGATG 4 cut(s) 322, 448, 470, 520
BstFNI CGCG 1 cut(s) 457
BstHHI GCGC 1 cut(s) 29
BstKTI GATC 2 cut(s) 521, 553
BstMBI GATC 2 cut(s) 518, 550
BstMWI GCNNNNNNNGC 1 cut(s) 484
BstNSI RCATGY 2 cut(s) 33, 482
BstUI CGCG 1 cut(s) 457
BstV1I GCAGC 1 cut(s) 499
BstV2I GAAGAC 1 cut(s) 425
BstX2I RGATCY 1 cut(s) 518
BstYI RGATCY 1 cut(s) 518
BsuRI GGCC 2 cut(s) 57, 298
BtgI CCRYGG 1 cut(s) 585
BtrI CACGTC 1 cut(s) 495
BtsCI GGATG 4 cut(s) 322, 448, 470, 520
BtsIMutI CAGTG 3 cut(s) 182, 337, 354
BveI ACCTGC 1 cut(s) 328
Cac8I GCNNGC 4 cut(s) 31, 55, 296, 480
CfoI GCGC 1 cut(s) 29
Cfr13I GGNCC 2 cut(s) 296, 582
Csp6I GTAC 1 cut(s) 205
CviAII CATG 4 cut(s) 30, 479, 586, 591
CviJI RGCY 8 cut(s) 8, 57, 138, 156, 251, 298, 347, 490
CviKI_1 RGCY 8 cut(s) 8, 57, 138, 156, 251, 298, 347, 490
CviQI GTAC 1 cut(s) 205
DpnI GATC 2 cut(s) 520, 552
DpnII GATC 2 cut(s) 518, 550
DraIII CACNNNGTG 1 cut(s) 360
EaeI YGGCCR 1 cut(s) 55
Eco130I CCWWGG 1 cut(s) 585
Eco47I GGWCC 1 cut(s) 582
EcoRI GAATTC 1 cut(s) 399
EcoT14I CCWWGG 1 cut(s) 585
ErhI CCWWGG 1 cut(s) 585
FaeI CATG 4 cut(s) 33, 482, 589, 594
FaiI YATR 6 cut(s) 31, 113, 115, 480, 587, 592
FalI AAGNNNNNCTT 2 cut(s) 240, 272
FaqI GGGAC 2 cut(s) 118, 558
FatI CATG 4 cut(s) 29, 478, 585, 590
FauI CCCGC 2 cut(s) 287, 377
FauNDI CATATG 1 cut(s) 113
Fnu4HI GCNGC 3 cut(s) 118, 121, 488
FokI GGATG 4 cut(s) 309, 455, 477, 527
Fsp4HI GCNGC 3 cut(s) 118, 121, 488
FspBI CTAG 1 cut(s) 153
GlaI GCGC 1 cut(s) 28
GluI GCNGC 3 cut(s) 118, 121, 488
HaeIII GGCC 2 cut(s) 57, 298
HhaI GCGC 1 cut(s) 29
Hin1II CATG 4 cut(s) 33, 482, 589, 594
Hin6I GCGC 1 cut(s) 27
HinP1I GCGC 1 cut(s) 27
HinfI GANTC 4 cut(s) 106, 452, 546, 556
HphI GGTGA 3 cut(s) 135, 296, 586
Hpy166II GTNNAC 1 cut(s) 243
Hpy188I TCNGA 2 cut(s) 304, 555
Hpy8I GTNNAC 1 cut(s) 243
Hpy99I CGWCG 1 cut(s) 496
HpyAV CCTTC 2 cut(s) 156, 272
HpyCH4III ACNGT 2 cut(s) 50, 332
HpyCH4IV ACGT 1 cut(s) 494
HpyCH4V TGCA 7 cut(s) 21, 33, 84, 168, 192, 482, 524
HpyF10VI GCNNNNNNNGC 1 cut(s) 484
HpySE526I ACGT 1 cut(s) 494
Hsp92II CATG 4 cut(s) 33, 482, 589, 594
HspAI GCGC 1 cut(s) 27
Kzo9I GATC 2 cut(s) 518, 550
LpnPI CCDG 8 cut(s) 31, 39, 170, 243, 323, 342, 389, 468
Lsp1109I GCAGC 1 cut(s) 499
LweI GCATC 1 cut(s) 533
MaeI CTAG 1 cut(s) 153
MaeII ACGT 1 cut(s) 494
MaeIII GTNAC 3 cut(s) 232, 308, 574
MalI GATC 2 cut(s) 520, 552
MboI GATC 2 cut(s) 518, 550
MboII GAAGA 1 cut(s) 430
MfeI CAATTG 1 cut(s) 163
MflI RGATCY 1 cut(s) 518
MhlI GDGCHC 1 cut(s) 224
MlsI TGGCCA 1 cut(s) 57
MluCI AATT 2 cut(s) 163, 399
MluNI TGGCCA 1 cut(s) 57
MlyI GAGTC 2 cut(s) 100, 540
MnlI CCTC 4 cut(s) 25, 125, 128, 372
Mox20I TGGCCA 1 cut(s) 57
MscI TGGCCA 1 cut(s) 57
MseI TTAA 1 cut(s) 392
MslI CAYNNNNRTG 2 cut(s) 304, 529
Msp20I TGGCCA 1 cut(s) 57
MunI CAATTG 1 cut(s) 163
MvnI CGCG 1 cut(s) 457
MwoI GCNNNNNNNGC 1 cut(s) 484
NcoI CCATGG 1 cut(s) 585
NdeI CATATG 1 cut(s) 113
NdeII GATC 2 cut(s) 518, 550
NlaIII CATG 4 cut(s) 33, 482, 589, 594
NmuCI GTSAC 2 cut(s) 308, 574
NspI RCATGY 2 cut(s) 33, 482
PaeI GCATGC 2 cut(s) 33, 482
PcsI WCGNNNNNNNCGW 1 cut(s) 93
PfeI GAWTC 2 cut(s) 452, 556
PflFI GACNNNGTC 1 cut(s) 580
PflMI CCANNNNNTGG 1 cut(s) 360
PkrI GCNGC 3 cut(s) 119, 122, 489
PleI GAGTC 2 cut(s) 100, 540
PpsI GAGTC 2 cut(s) 100, 540
PspPI GGNCC 2 cut(s) 296, 582
PsuI RGATCY 1 cut(s) 518
PsyI GACNNNGTC 1 cut(s) 580
RsaI GTAC 1 cut(s) 206
RsaNI GTAC 1 cut(s) 205
RseI CAYNNNNRTG 2 cut(s) 304, 529
SaqAI TTAA 1 cut(s) 392
SatI GCNGC 3 cut(s) 118, 121, 488
Sau3AI GATC 2 cut(s) 518, 550
Sau96I GGNCC 2 cut(s) 296, 582
SchI GAGTC 2 cut(s) 100, 540
SduI GDGCHC 1 cut(s) 224
SetI ASST 7 cut(s) 41, 148, 342, 383, 497, 565, 570
SfaNI GCATC 1 cut(s) 533
SinI GGWCC 1 cut(s) 582
SmiMI CAYNNNNRTG 2 cut(s) 304, 529
SphI GCATGC 2 cut(s) 33, 482
Sse9I AATT 2 cut(s) 163, 399
SsiI CCGC 6 cut(s) 117, 120, 123, 282, 294, 384
SspMI CTAG 1 cut(s) 153
StyI CCWWGG 1 cut(s) 585
TaaI ACNGT 2 cut(s) 50, 332
TaiI ACGT 1 cut(s) 497
TaqI TCGA 2 cut(s) 377, 549
TasI AATT 2 cut(s) 163, 399
TauI GCSGC 2 cut(s) 120, 123
TfiI GAWTC 2 cut(s) 452, 556
Tru1I TTAA 1 cut(s) 392
Tru9I TTAA 1 cut(s) 392
TscAI CASTG 3 cut(s) 189, 337, 361
TseFI GTSAC 2 cut(s) 308, 574
TseI GCWGC 1 cut(s) 487
Tsp45I GTSAC 2 cut(s) 308, 574
TspDTI ATGAA 1 cut(s) 459
TspRI CASTG 3 cut(s) 189, 337, 361
Tth111I GACNNNGTC 1 cut(s) 580
Van91I CCANNNNNTGG 1 cut(s) 360
VpaK11BI GGWCC 1 cut(s) 582
XapI RAATTY 1 cut(s) 399
XceI RCATGY 2 cut(s) 33, 482
XspI CTAG 1 cut(s) 153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.