Rorug05G0312800

Kiwellin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0012598
Physical Location & Seq
Forward (+)
36979544 .. 36982680
3137 bp
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UTR
Exon/CDS
Intron
Rorug05G0312800.1

Sequence Viewer

Length: 399 bp
ATGGCAGCTGAGGATTCCCAGAGATTGCAAGTTCGAGAAGCATTTATGATGGGATACCGTGATGGTGTTATAGCCGGAAAGGAAGCTTCTTCTCAGGAGGGATTCAATATTGACTTTAAGCAATCACTCCTTGTTGGTTACAACTGGGGTCTTGTGAGAGGTGTTACAAGTACATTGGCTTCCCTTCCAGATGGTAAAAGGGAGAAGTTGATTGAAACAGAAGAACAAAGAACTCGATTTCAGGAATTAGAACAAGTTGAAGGTTCAGAGGCTAGTTCTTTTGAAGTTGAAACTGGTCTGGAAGAGCAAAGATTAGACCACAGTGGTCTAGGAAATTACTATGCAAAGCTCCGTTCACTTCTTTTGGAGTCGCCTGGAATCAAAGTCAAGTTCCCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

132

Amino Acids

14.81

Weight (kDa)

4.9

Isoelectric Point (pI)

50.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Yae1_N PF09811 18 - 55 1.7e-06 Essential protein Yae1, N terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AfaI GTAC 1 cut(s) 172
AgsI TTSAA 5 cut(s) 106, 215, 260, 284, 290
AjnI CCWGG 1 cut(s) 373
AluBI AGCT 3 cut(s) 8, 86, 349
AluI AGCT 3 cut(s) 8, 86, 349
ApeKI GCWGC 1 cut(s) 5
BarI GAAGNNNNNNTAC 2 cut(s) 163, 195
BbvCI CCTCAGC 1 cut(s) 9
BbvI GCAGC 1 cut(s) 17
BccI CCATC 3 cut(s) 43, 56, 185
BciT130I CCWGG 1 cut(s) 375
BciVI GTATCC 1 cut(s) 47
BfaI CTAG 3 cut(s) 273, 329, 397
BfuI GTATCC 1 cut(s) 47
BisI GCNGC 1 cut(s) 6
BlsI GCNGC 1 cut(s) 7
Bme1390I CCNGG 1 cut(s) 375
BmrFI CCNGG 1 cut(s) 375
BmrI ACTGGG 1 cut(s) 154
BmuI ACTGGG 1 cut(s) 154
Bpu10I CCTNAGC 1 cut(s) 9
Bse1I ACTGG 2 cut(s) 149, 298
BseBI CCWGG 1 cut(s) 375
BseMII CTCAG 1 cut(s) 107
BseNI ACTGG 2 cut(s) 149, 298
BseXI GCAGC 1 cut(s) 17
BsiSI CCGG 1 cut(s) 75
BspCNI CTCAG 1 cut(s) 106
BspQI GCTCTTC 1 cut(s) 297
BsrI ACTGG 2 cut(s) 149, 298
Bst2UI CCWGG 1 cut(s) 375
Bst4CI ACNGT 2 cut(s) 59, 323
Bst6I CTCTTC 1 cut(s) 297
BstDEI CTNAG 2 cut(s) 9, 93
BstNI CCWGG 1 cut(s) 375
BstSCI CCNGG 1 cut(s) 373
BstV1I GCAGC 1 cut(s) 17
BsuI GTATCC 1 cut(s) 47
BtsIMutI CAGTG 1 cut(s) 328
Csp6I GTAC 1 cut(s) 171
CviJI RGCY 6 cut(s) 8, 74, 86, 179, 272, 349
CviKI_1 RGCY 6 cut(s) 8, 74, 86, 179, 272, 349
CviQI GTAC 1 cut(s) 171
DdeI CTNAG 2 cut(s) 9, 93
Eam1104I CTCTTC 1 cut(s) 297
EarI CTCTTC 1 cut(s) 297
EcoRII CCWGG 1 cut(s) 373
FaiI YATR 3 cut(s) 47, 71, 342
Fnu4HI GCNGC 1 cut(s) 6
Fsp4HI GCNGC 1 cut(s) 6
FspBI CTAG 3 cut(s) 273, 329, 397
GluI GCNGC 1 cut(s) 6
HapII CCGG 1 cut(s) 75
HindIII AAGCTT 1 cut(s) 84
HinfI GANTC 4 cut(s) 14, 102, 368, 378
HpaII CCGG 1 cut(s) 75
Hpy166II GTNNAC 1 cut(s) 356
Hpy188I TCNGA 1 cut(s) 268
Hpy188III TCNNGA 5 cut(s) 35, 95, 188, 242, 299
Hpy8I GTNNAC 1 cut(s) 356
HpyAV CCTTC 2 cut(s) 194, 254
HpyCH4III ACNGT 2 cut(s) 59, 323
HpyCH4V TGCA 2 cut(s) 28, 344
HpyF3I CTNAG 2 cut(s) 9, 93
LguI GCTCTTC 1 cut(s) 297
LmnI GCTCC 1 cut(s) 354
Lsp1109I GCAGC 1 cut(s) 17
MaeI CTAG 3 cut(s) 273, 329, 397
MaeIII GTNAC 2 cut(s) 137, 163
MboII GAAGA 3 cut(s) 81, 233, 314
MluCI AATT 2 cut(s) 245, 334
MlyI GAGTC 1 cut(s) 377
MnlI CCTC 4 cut(s) 4, 91, 152, 262
MseI TTAA 1 cut(s) 117
MspA1I CMGCKG 1 cut(s) 8
MspI CCGG 1 cut(s) 75
MspR9I CCNGG 1 cut(s) 375
MvaI CCWGG 1 cut(s) 375
PciSI GCTCTTC 1 cut(s) 297
PfeI GAWTC 3 cut(s) 14, 102, 378
PkrI GCNGC 1 cut(s) 7
PleI GAGTC 1 cut(s) 376
PpsI GAGTC 1 cut(s) 376
Psp6I CCWGG 1 cut(s) 373
PspGI CCWGG 1 cut(s) 373
PvuII CAGCTG 1 cut(s) 8
RsaI GTAC 1 cut(s) 172
RsaNI GTAC 1 cut(s) 171
SapI GCTCTTC 1 cut(s) 297
SaqAI TTAA 1 cut(s) 117
SatI GCNGC 1 cut(s) 6
SchI GAGTC 1 cut(s) 377
ScrFI CCNGG 1 cut(s) 375
SetI ASST 5 cut(s) 10, 88, 163, 265, 351
Sse9I AATT 2 cut(s) 245, 334
SspI AATATT 1 cut(s) 109
SspMI CTAG 3 cut(s) 273, 329, 397
StyD4I CCNGG 1 cut(s) 373
TaaI ACNGT 2 cut(s) 59, 323
TaqI TCGA 2 cut(s) 34, 235
TasI AATT 2 cut(s) 245, 334
TatI WGTACW 1 cut(s) 170
TfiI GAWTC 3 cut(s) 14, 102, 378
Tru1I TTAA 1 cut(s) 117
Tru9I TTAA 1 cut(s) 117
TscAI CASTG 1 cut(s) 328
TseI GCWGC 1 cut(s) 5
TspGWI ACGGA 1 cut(s) 341
TspRI CASTG 1 cut(s) 328
XspI CTAG 3 cut(s) 273, 329, 397
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.